Fish oil anthocyanin-loaded self-emulsifying system based on EPA (eicosapentaenoic acid) and preparation method thereof
The preparation method of EPA fish oil-loaded anthocyanin self-emulsification system has solved the problems of low preparation efficiency and low bioavailability of anthocyanin delivery systems, realizing the efficient application of anthocyanins in the fields of cosmetics, food and pharmaceuticals.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-22
- Publication Date
- 2026-03-24
AI Technical Summary
Existing anthocyanin delivery systems have low preparation efficiency and low bioavailability, which limits their application, especially in the food industry.
The preparation method of EPA fish oil-loaded anthocyanin self-emulsifying system involves mixing oil phase, water, surfactant, eutectic solvent and anthocyanin in a preset ratio, heating to a preset temperature, holding at the temperature and then cooling to room temperature to form a self-emulsifying system without obvious stratification.
It significantly improves the bioavailability of anthocyanins, broadens their application scope in cosmetics, food and pharmaceuticals, and is simple and environmentally friendly to operate.
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Figure CN121714036A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of food preparation, in particular to a self-emulsifying system for loading anthocyanins based on EPA fish oil and a preparation method thereof. BACKGROUND
[0002] Anthocyanins, as a class of water-soluble pigments widely existing in fruits and vegetables, belong to the category of flavonoids and have a typical C6-C3-C6 basic skeleton structure. Common dietary anthocyanins include pelargonidin, cyanidin, delphinidin, peony pigment, petunidin and malvidin, among which the most representative component is cyanidin-3-O-glucoside (C3G). The molecular structure of anthocyanins will undergo reversible proton transfer and structural rearrangement with changes in environmental pH, and thus exhibit color changes: relatively stable under acidic conditions, showing red color; while under neutral and alkaline conditions, the stability is poor, and the color gradually changes from purple to blue-green. Anthocyanins have significant antioxidant and anti-inflammatory activities and are believed to have certain value in the prevention of chronic diseases. The Recommended Nutrient Intake for Chinese Residents (2023 Edition) sets the recommended value of anthocyanin intake at 50 mg / day, which confirms its important health significance in daily diet. However, anthocyanins are sensitive to environmental factors such as temperature, light and oxygen, and are prone to degradation. In the absorption process, anthocyanins are easily inactivated by enzymes and microorganisms in the gastrointestinal tract. The hydrophilic nature of anthocyanins also hinders their effective penetration of the lipid bilayer of small intestinal epithelial cells, resulting in low oral bioavailability.
[0003] Embedding technology is an effective strategy to improve the stability and bioavailability of anthocyanins, and constructing liposomes is a commonly used method. Liposomes are microcapsules with a bilayer structure, similar to biological membranes, which can encapsulate water-soluble active ingredients and have the characteristics of sustained release, long-acting and good cell affinity. However, the preparation process of liposomes usually involves multiple steps such as film dispersion, extrusion and ultrasonication, which requires high equipment. In addition, organic reagents such as ethanol are often introduced during preparation, which limits their wide application in the food industry.
[0004] Emulsion systems are an alternative because of their simpler process and better adaptation to food matrices. However, current emulsion systems have limitations in loading anthocyanins, and the high water content inside the emulsion can easily reduce the stability of the emulsion and accelerate the degradation of anthocyanins. SUMMARY
[0005] The main purpose of the present application is to provide a self-emulsifying system for loading anthocyanins based on EPA fish oil and a preparation method thereof, aiming to solve the technical problems of low preparation efficiency and low bioavailability of anthocyanin delivery systems in the prior art.
[0006] To achieve the above object, the technical solutions adopted by the embodiments of the present application are as follows: In a first aspect, the embodiments of the present application provide a preparation method of an EPA fish oil-loaded anthocyanin self-emulsifying system, comprising the following steps: The oil phase, water, surfactant, eutectic solvent and anthocyanin are mixed in a preset ratio, heated to a preset temperature, incubated for a preset time, cooled to room temperature, and an EPA fish oil-loaded anthocyanin self-emulsifying system without obvious stratification is obtained.
[0007] As some optional embodiments of the present application, in the preparation of 1 part by weight of the EPA fish oil-loaded anthocyanin self-emulsifying system, the preset ratio of the oil phase, water, surfactant, eutectic solvent and anthocyanin is 0.01-0.5 parts by weight: 0.01-0.5 parts by weight: 0.01-0.5 parts by weight: 0.01-0.5 parts by weight: 0.01-0.5 parts by weight.
[0008] As some optional embodiments of the present application, in the preparation of 1 part by weight of the EPA fish oil-loaded anthocyanin self-emulsifying system, the preset ratio of the oil phase, water, surfactant, eutectic solvent and anthocyanin is 0.14 parts by weight: 0.15 parts by weight: 0.2-0.25 parts by weight: 0.21-0.26 parts by weight: 0.2-0.25 parts by weight.
[0009] As some optional embodiments of the present application, the oil phase is at least one of medium-chain triglyceride, fish oil with EPA=30%-99%, conjugated linoleic acid and tocopheryl acetate.
[0010] As some optional embodiments of the present application, the surfactant is at least one of monoglyceride, polysorbate-80, sorbitan oleate, gum arabic, soybean lecithin and sucrose fatty acid ester.
[0011] As some optional embodiments of the present application, the eutectic solvent comprises at least one of choline chloride-propylene glycol and betaine-propylene glycol.
[0012] As some optional embodiments of the present application, the preset temperature is 40-100ºC, and the preset time is 5-15 min.
[0013] In a second aspect, the embodiments of the present application also provide an EPA fish oil-loaded anthocyanin self-emulsifying system prepared by the above method.
[0014] In a third aspect, the embodiments of the present application also provide an application of the EPA fish oil-loaded anthocyanin self-emulsifying system as described above, which is used for preparing cosmetics, food and drugs.
[0015] Compared with the existing technical means, the present application provides a method for preparing a low eutectic solvent self-emulsifying system with high anthocyanin bioavailability. Specifically, the operation process of the method is to accurately mix the oil phase, water, surfactant, low eutectic solvent and anthocyanin according to the pre-set ratio, then heat the mixture to a specific pre-set temperature, and naturally cool to room temperature after heating is completed. Through this series of steps, an EPA fish oil-loaded anthocyanin self-emulsifying system without obvious stratification phenomenon can be finally obtained. As can be seen from the above process, the method adopted by the present application uses green solvent as a co-surfactant and a dissolution medium for anthocyanin, which is not only green and environmentally friendly, but also simple and easy to operate, without the need for any special equipment. This method significantly improves the bioavailability of anthocyanin, thereby greatly widening its application range in the fields of cosmetics, food and pharmaceuticals. BRIEF DESCRIPTION OF DRAWINGS
[0016] In order to more clearly illustrate the technical solutions in the specific embodiments or prior art of the present application, the drawings needed in the specific embodiments or prior art description will be briefly introduced below. In all the drawings, similar elements or parts are generally denoted by similar reference numerals.
[0017] Figure 1 A standard curve for anthocyanin quantification related to the embodiments of the present application; Figure 2 A visual graph of the anthocyanin self-emulsifying system related to the embodiments of the present application; Figure 3 A visual graph of the anthocyanin self-emulsifying system diluted 100 times related to the embodiments of the present application; Figure 4 A result graph of the in vitro simulated digestion retention rate of the anthocyanin self-emulsifying system related to the embodiments of the present application; Figure 5 A result graph of the in vitro simulated digestion retention rate of anthocyanin in a double oil phase self-emulsifying system related to the embodiments of the present application.
[0018] The implementation of the present application, functional characteristics and advantages will be further described with reference to the embodiments and the accompanying drawings. DETAILED DESCRIPTION
[0019] With reference to the drawings of the embodiments of the present application, the technical solutions in the embodiments of the present application will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments of the present application, all other embodiments obtained by a person of ordinary skill in the art without creative work fall within the scope of protection of the present application.
[0020] As described above, anthocyanins have significant antioxidant and anti-inflammatory activities and are considered valuable in preventing chronic diseases. However, anthocyanins have generally low oral bioavailability due to their sensitivity to the environment, easy degradation in the gastrointestinal tract, and hydrophilicity hindering transmembrane absorption. Emulsion systems are widely studied as a delivery solution with simple process and good food compatibility, and many prior arts have improved the storage stability and encapsulation efficiency of anthocyanins through different matrices and processes. However, the high water content, limited loading capacity, and easy degradation limit their application. Self-emulsifying systems show significant potential with high loading capacity, storage stability, and easy soft capsule packaging and controlled release.
[0021] That is, the present application provides a method for preparing a high-anthocyanin bioavailability eutectic solvent self-emulsifying system to solve the problems of low preparation efficiency and limited bioavailability improvement of anthocyanin emulsion. The oil phase, water, surfactant, eutectic solvent, and anthocyanin are mixed at a predetermined ratio, heated to a predetermined temperature, incubated for a predetermined time, and cooled to room temperature to obtain an EPA fish oil-based anthocyanin self-emulsifying system without obvious stratification.
[0022] Specifically, the method for preparing an EPA fish oil-based anthocyanin self-emulsifying system provided by the embodiments of the present application comprises the following steps: The oil phase, water, surfactant, eutectic solvent, and anthocyanin are mixed at a predetermined ratio, heated to a predetermined temperature, incubated for a predetermined time, and cooled to room temperature to obtain an EPA fish oil-based anthocyanin self-emulsifying system without obvious stratification.
[0023] More specifically, in the preparation of 1 part by weight of the EPA fish oil-based anthocyanin self-emulsifying system, the predetermined ratio of the oil phase, water, surfactant, eutectic solvent, and anthocyanin is 0.01-0.5 parts by weight: 0.01-0.5 parts by weight: 0.01-0.5 parts by weight: 0.01-0.5 parts by weight: 0.01-0.5 parts by weight.
[0024] More specifically, the preset ratio of the oil phase, the water, the surfactant, the low eutectic solvent and the anthocyanin is 0.14 parts by weight: 0.15 parts by weight: 0.2 parts by weight-0.25 parts by weight: 0.21 parts by weight-0.26 parts by weight: 0.2 parts by weight-0.25 parts by weight, per 1 part by weight of the EPA fish oil-based anthocyanin self-emulsifying system in preparation.
[0025] More specifically, the preset temperature is 40ºC-100ºC, and the preset time is 5 min-15 min.
[0026] More specifically, the oil phase is at least one of medium-chain triglyceride, fish oil with EPA=30%-99%, conjugated linoleic acid and tocopheryl acetate; the surfactant is at least one of monoglyceride, polysorbate-80, sorbitan oleate, gum arabic, soy lecithin and sucrose fatty acid ester; and the low eutectic solvent includes at least one of choline chloride-propylene glycol and betaine-propylene glycol.
[0027] To facilitate those skilled in the art to understand the technical solutions of the present application, the method described in the present application is described in detail below in conjunction with examples: Example 1: The oil phase, water, surfactant, low eutectic solvent and anthocyanin are mixed, heated at 50ºC for 10 min, vortexed and uniformly mixed, and then cooled to room temperature to obtain an anthocyanin-loaded emulsion without obvious stratification.
[0028] In this example, the oil phase is 0.14 parts by weight of fish oil (EPA=70%); the surfactant is 0.1 parts by weight of sorbitan oleate and 0.1 parts by weight of gum arabic; the low eutectic solvent is 0.26 parts by weight of choline chloride-propylene glycol; the water is 0.15 parts by weight; and the anthocyanin is 0.25 parts by weight.
[0029] Example 2: The oil phase, water, surfactant, low eutectic solvent and anthocyanin are mixed, heated at 50ºC for 10 min, vortexed and uniformly mixed, and then cooled to room temperature to obtain an anthocyanin-loaded emulsion without obvious stratification.
[0030] In this example, the oil phase is 0.14 parts by weight of fish oil (EPA=80%); the surfactant is 0.1 parts by weight of soy lecithin and 0.1 parts by weight of sucrose fatty acid ester; the low eutectic solvent is 0.26 parts by weight of choline chloride-propylene glycol; the water is 0.15 parts by weight; and the anthocyanin is 0.25 parts by weight.
[0031] Example 3: The oil phase, water, surfactant, low eutectic solvent and anthocyanin are mixed, heated at 50ºC for 10 min, vortexed and uniformly mixed, and then cooled to room temperature to obtain an anthocyanin-loaded emulsion without obvious stratification.
[0032] In this embodiment, the oil phase is 0.14 parts by weight of fish oil (EPA = 80%); the surfactant is 0.1 parts by weight of gum arabic and 0.1 parts by weight of soybean lecithin; the deep eutectic solvent is 0.26 parts by weight of betaine-propylene glycol; the parts by weight of water is 0.15; and the parts by weight of anthocyanin is 0.25.
[0033] In this embodiment, the oil phase is 0.14 parts by weight of fish oil (EPA = 80%); the surfactant is 0.1 parts by weight of gum arabic and 0.1 parts by weight of soybean lecithin; the deep eutectic solvent is 0.26 parts by weight of betaine-propylene glycol; the parts by weight of water is 0.15; and the parts by weight of anthocyanin is 0.25.
[0034] In this embodiment, the oil phase is 0.14 parts by weight of fish oil (EPA = 80%); the surfactant is 0.1 parts by weight of gum arabic and 0.1 parts by weight of soybean lecithin; the deep eutectic solvent is 0.26 parts by weight of betaine-propylene glycol; the parts by weight of water is 0.15; and the parts by weight of anthocyanin is 0.25.
[0035] In this embodiment, the oil phase is 0.14 parts by weight of fish oil (EPA = 80%); the surfactant is 0.1 parts by weight of gum arabic and 0.1 parts by weight of soybean lecithin; the deep eutectic solvent is 0.26 parts by weight of betaine-propylene glycol; the parts by weight of water is 0.15; and the parts by weight of anthocyanin is 0.25.
[0036] In this embodiment, the oil phase is 0.14 parts by weight of fish oil (EPA = 80%); the surfactant is 0.1 parts by weight of gum arabic and 0.1 parts by weight of soybean lecithin; the deep eutectic solvent is 0.26 parts by weight of betaine-propylene glycol; the parts by weight of water is 0.15; and the parts by weight of anthocyanin is 0.25.
[0037] In this embodiment, the oil phase is 0.14 parts by weight of fish oil (EPA = 80%); the surfactant is 0.1 parts by weight of gum arabic and 0.1 parts by weight of soybean lecithin; the deep eutectic solvent is 0.26 parts by weight of betaine-propylene glycol; the parts by weight of water is 0.15; and the parts by weight of anthocyanin is 0.25.
[0038] In this embodiment, the oil phase is 0.14 parts by weight of fish oil (EPA = 80%); the surfactant is 0.1 parts by weight of gum arabic and 0.1 parts by weight of soybean lecithin; the deep eutectic solvent is 0.26 parts by weight of betaine-propylene glycol; the parts by weight of water is 0.15; and the parts by weight of anthocyanin is 0.25.
[0039] In this embodiment, the oil phase is 0.14 parts by weight of fish oil (EPA = 80%); the surfactant is 0.1 parts by weight of gum arabic and 0.1 parts by weight of soybean lecithin; the deep eutectic solvent is 0.26 parts by weight of betaine-propylene glycol; the parts by weight of water is 0.15; and the parts by weight of anthocyanin is 0.25.
[0040] In this example, the oil phase is 0.14 parts by weight of medium-chain fatty acid ester and 0.1 parts by weight of tocopherol acetate; the surfactant is 0.125 parts by weight of polysorbate-80 and 0.125 parts by weight of sorbitan oleate; the low eutectic solvent is 0.21 parts by weight of choline chloride-propylene glycol; the parts by weight of water is 0.1; and the parts by weight of anthocyanin is 0.2.
[0041] In this comparative example, the oil phase is 0.14 parts by weight of fish oil (EPA = 80%); the surfactant is 0.1 parts by weight of polysorbate-80 and 0.1 parts by weight of soybean lecithin; the parts by weight of water is 0.41; and the parts by weight of anthocyanin is 0.25, without the remaining components.
[0042] The remaining steps are the same as in Example 1.
[0043] In this comparative example, the oil phase is 0.3 parts by weight of flaxseed oil; the surfactant is 0.2 parts by weight of sunflower lecithin; and 0.5 parts by weight of blueberry extract (containing 0.015 parts by weight of anthocyanin), without the remaining components.
[0044] The remaining steps are the same as in Example 1.
[0045] In this comparative example, the oil phase is 0.14 parts by weight of fish oil (EPA = 80%); the parts by weight of water is 0.61; and the parts by weight of anthocyanin is 0.25, without the remaining components.
[0046] The remaining steps are the same as in Example 1.
[0047] In this comparative example, the parts by weight of water is 0.75; and the parts by weight of anthocyanin is 0.25, without the remaining components.
[0048] The remaining steps are the same as in Example 1.
[0049] In this comparative example, the parts by weight of water is 0.8; and the parts by weight of anthocyanin is 0.2, without the remaining components.
[0050] The remaining steps are the same as in Example 1.
[0051] The performance of the EPA fish oil-based anthocyanin self-emulsifying systems obtained in Examples 1-7 and Comparative Examples 1-5 will be tested as follows, as shown in Experimental Examples 1-3: Experimental Example 1: A series of mass of anthocyanin powder was weighed and dissolved in deionized water to form an anthocyanin solution, and the solid-liquid ratio was 0.02:1, 0.03:1, 0.04:1, 0.05:1, 0.06:1, 0.08:1, 0.1:1, 0.12:1, 0.15:1, 0.18:1, respectively. The prepared anthocyanin solution was mixed with KCl-HCl buffer solution with pH 1.0 at a mixing volume ratio of 1:2. At the same time, the prepared anthocyanin solution was mixed with sodium acetate-acetic acid buffer solution with pH 4.5 at a mixing volume ratio of 1:2. The absorbance values of anthocyanin at 520 nm and 700 nm in the buffer solutions with pH 1.0 and pH 4.5 were measured, and the standard curve of anthocyanin content was plotted as pH 1.0 (A 520 -A 700 ) - pH 4.5 (A 520 -A 700 ). The standard curve is shown in Figure 1 .
[0052] Experimental Example 2: As shown in Figure 2 , examples 1-5 can all form stable anthocyanin-loaded systems, while comparative example 1 shows obvious stratification without the addition of a eutectic solvent, which indicates the importance of the eutectic solvent as a co-surfactant for the stability of the self-emulsifying system.
[0053] As shown in Figure 3 , after 100-fold dilution of the anthocyanin-loaded self-emulsifying system, examples 1-5 form a uniformly dispersed emulsion, while comparative example 2 cannot be miscible with water after dilution, which indicates that the self-emulsifying system forms an internal dense oil-water interface structure after dilution.
[0054] Experimental Example 3: According to the INFOGEST static digestion model, simulated gastric and intestinal electrolyte buffer solutions were prepared. 1 part by weight of the anthocyanin-loaded self-emulsifying system was diluted 5 times with deionized water, and the same volume of simulated gastric buffer and trace CaCl2 was added. According to the mass of anthocyanin, 60 wt% of pepsin was added. The system entered the 60 min gastric digestion period. The same volume of simulated intestinal buffer and trace CaCl2 was added. According to the amount of oil in the system, 12 wt% of porcine bile salt, 4 wt% of trypsin, and 10 wt% of pancreatic lipase were added. The system entered the 180 min intestinal digestion period. Samples were taken during the gastric and intestinal digestion periods, and the retention rate of anthocyanin during digestion was determined.
[0055] As shown in Figure 4As shown, the retention rate of anthocyanins in the gastric digestion period is maintained above 90%, indicating that it has good stability in the acidic environment. This result is consistent with the conclusion that anthocyanins are structurally stable under low pH conditions reported in the literature. After entering the intestinal digestion stage, the retention rate of anthocyanins in Comparative Example 3 and Comparative Example 4 decreases from about 95% to 12%-16%. The retention rate of Examples 1-5 decreases to 50%-60%, which is about 4 times higher than that of the comparative examples, indicating that the self-emulsifying system has a significant protective effect on anthocyanins. Notably, the retention rate of Comparative Example 2 decreases to 24.86%, indicating that the protective effect of Examples 1-5 on anthocyanins is much higher than that of the comparative products.
[0056] Based on the above-mentioned best formula for protection effect, the present application additionally adds different oil phases to the protection effect of anthocyanins in gastrointestinal digestion. As shown in Table 2, the retention rate of anthocyanins in the self-emulsifying system of Examples 1-5 is about 50%-60%, which is about 4 times higher than that of Comparative Example 5. This indicates that the self-emulsifying system has a significant protective effect on anthocyanins. Figure 5 As shown, the retention rate of Examples 6-7 increases to about 65% after adding conjugated linoleic acid and tocopherol acetate, respectively, which is 4.3 times higher than that of Comparative Example 5. This indicates that the additional oil phase may promote the formation of finer oil droplets in the self-emulsifying system, thereby improving the retention rate of anthocyanins.
[0057] As can be seen, the technical solution of the present application successfully improves the retention rate of anthocyanins in the gastrointestinal digestion process by designing a low eutectic solvent self-emulsifying system for anthocyanins, and through a simplified preparation process, its application in multiple fields is more promising.
[0058] Compared with the prior art, the technical solution described in the present application presents technical progress: 1. Significant increase in anthocyanin loading: The present application takes advantage of the low eutectic solvent to act as a co-surfactant and a solubilizing medium for anthocyanins, significantly enhancing the carrying capacity of the self-emulsifying system for anthocyanins. For example, in Examples 1-5 using choline chloride-propylene glycol or betaine-propylene glycol low eutectic solvents, each formulation can carry 0.25 parts by weight of anthocyanins, and maintain the uniformity and stability of the system, while the carrying capacity of conventional methods is usually only between 0.01 parts by weight and 0.05 parts by weight. This may be due to the hydrogen bond interaction between the low eutectic solvent and the anthocyanin molecules, which allows for efficient dispersion and stable dissolution under low water content conditions.
[0059] 2. Improvement of anthocyanin bioavailability: The present application improves the retention rate of anthocyanins in the gastrointestinal digestion process by diluting the self-emulsifying emulsion. For example, the retention rate of anthocyanins in Example 7 is 66.20%, which is about 4.3 times higher than that of free anthocyanins in Comparative Example 5 (15.71%). This effect is attributed to the formation of nano-micro emulsion structure and mixed micelles co-assembled with bile salts in the system during digestion, which can achieve efficient encapsulation and protection of anthocyanins, thereby enhancing their stability and promoting intestinal absorption.
[0060] 3. Easy to operate and environmentally friendly: compared with the traditional methods of building liposomes and emulsion systems, the technical solution of the present application is easy to operate and does not depend on complex equipment. The whole process mainly covers heating and vortex dissolution, which makes the preparation process more efficient and low-cost. The eutectic solvent used in this method belongs to a green and environmentally friendly solvent system (such as natural ingredients such as choline chloride and betaine), avoiding the use of harmful chemical solvents, and meeting the requirements of modern pharmaceutical, food and cosmetic industries for green and environmentally friendly, low pollution and low cost.
[0061] In summary, the present application introduces eutectic solvent as a co-surfactant and anthocyanin dissolving medium, which has made significant progress in anthocyanin carrying capacity, gastrointestinal retention rate and process operability. This technical solution effectively overcomes the limitations of existing delivery systems, providing more reliable technical support for the large-scale application of anthocyanins in the fields of food, medicine and cosmetics.
[0062] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present application shall be included in the protection scope of the present application.
Claims
1. A preparation method based on an EPA-loaded anthocyanin self-emulsification system, characterized in that, Includes the following steps: The oil phase, water, surfactant, eutectic solvent and anthocyanin are mixed in a preset ratio, heated to a preset temperature, kept at the temperature for a preset time, and cooled to room temperature to obtain a self-emulsifying system based on EPA fish oil and anthocyanins without obvious stratification.
2. The preparation method based on the EPA fish oil anthocyanin self-emulsifying system according to claim 1, characterized in that, When preparing each part by weight of the EPA fish oil-based anthocyanin self-emulsifying system, the preset ratio of the oil phase, the water, the surfactant, the eutectic solvent, and the anthocyanin is 0.01 parts by weight - 0.5 parts by weight: 0.01 parts by weight - 0.5 parts by weight: 0.01 parts by weight - 0.5 parts by weight: 0.01 parts by weight - 0.5 parts by weight: 0.01 parts by weight - 0.5 parts by weight.
3. The preparation method based on the EPA fish oil anthocyanin self-emulsifying system according to claim 1, characterized in that, When preparing each part by weight of the EPA fish oil-based anthocyanin self-emulsifying system, the preset ratio of the oil phase, the water, the surfactant, the eutectic solvent, and the anthocyanin is 0.14 parts by weight: 0.15 parts by weight: 0.2 parts by weight - 0.25 parts by weight: 0.21 parts by weight - 0.26 parts by weight: 0.2 parts by weight - 0.25 parts by weight.
4. The preparation method based on the EPA fish oil anthocyanin self-emulsifying system according to claim 1, characterized in that, The oil phase is at least one of medium-chain triglycerides, fish oil with EPA of 30%-99%, conjugated linoleic acid, and tocopheryl acetate.
5. The preparation method based on the EPA fish oil anthocyanin self-emulsifying system according to claim 1, characterized in that, The surfactant is at least one of mono-fatty acid glycerides, polysorbate-80, sorbitan oleate, gum arabic, soybean lecithin, and sucrose fatty acid esters.
6. The preparation method based on the EPA fish oil anthocyanin self-emulsifying system according to claim 1, characterized in that, The eutectic solvent includes at least one of choline chloride-propylene glycol and betaine-propylene glycol.
7. The preparation method based on the EPA fish oil anthocyanin self-emulsifying system according to claim 1, characterized in that, The preset temperature is 40℃-100℃, and the preset time is 5min-15min.
8. A self-emulsifying system based on EPA fish oil and anthocyanins, characterized in that, Prepared by the method described in any one of claims 1-7.
9. An application of the EPA-based anthocyanin-loaded self-emulsifying system as described in claim 8, characterized in that, The EPA-based anthocyanin self-emulsifying system is used to prepare cosmetics, food, and pharmaceuticals.