In-vitro fermentation device for simulating ruminant rumen internal environment

Abstract

The invention relates to the technical field of ruminant nutrition and feed science, in particular to an in-vitro fermentation device for simulating ruminant rumen internal environment. The in-vitro fermentation device comprises a box body, and a digestion chamber is arranged in the middle of the box body and used for simulating the internal environment of a rumen; the fermentation bottle is rotatably arranged in the digestion chamber and is used for simulating the wriggling effect of the rumen; the temperature control module is used for adjusting the internal temperature of the digestion chamber; the rotating speed control module is used for driving the fermentation bottle to rotate; the gas management module is used for maintaining an anaerobic environment; wherein the multiple fermentation bottles are arranged in the digestion chamber in an array mode, and the rotating speed control module is provided with a transmission assembly and used for driving the multiple fermentation bottles to rotate synchronously. According to the in-vitro fermentation device disclosed by the invention, the in-vitro fermentation device can truly simulate the rumen degradation process of feed raw materials, and provides a scientific basis for precise preparation of daily ration of ruminants, optimization of a feed formula and improvement of animal production performance.

Description

Technical Field

[0001] This invention relates to the field of ruminant nutrition and feed science and technology, and in particular to an in vitro fermentation device for simulating the rumen environment of ruminants. Background Technology

[0002] The rumen of ruminants (such as cattle and sheep) is a complex anaerobic fermentation ecosystem. Its internal environment (temperature, pH, anaerobic conditions, microbial community, and mechanical peristalsis) directly affects the digestibility of feed, thus determining the animal's production performance (such as milk production and daily weight gain). In the precise formulation of ruminant diets, accurately assessing the rumen degradation rate (such as dry matter degradation rate and crude protein degradation rate) of feed ingredients or total mixed rations (TMR) is a key basis.

[0003] Currently, in vitro rumen-simulated fermentation technology is the main method for studying the rumen degradation characteristics of feed, but existing devices generally have the following drawbacks: Insufficient realism in environmental simulation: Most devices can only control temperature and cannot accurately simulate the anaerobic environment, mechanical peristalsis and gas dynamic balance of the rumen (such as the accumulation of methane, carbon dioxide and other substances may inhibit rumen microbial fermentation, resulting in inaccurate data results, or even the risk of explosion). Temperature control has limitations: traditional heating methods usually use electric heating wires, which can generate open flames or localized high temperatures, posing a safety hazard when in contact with methane produced during fermentation; and they lack effective cooling functions, making it difficult to maintain a stable operating temperature in hot summer regions, thus hindering effective fermentation. Poor fermentation uniformity: The use of independent control of individual bottles or asynchronous rotation leads to large differences in the internal environment of each fermentation bottle, which cannot truly reflect the uniform fermentation state of the rumen population. Lack of gas management: The lack of an automatic venting function allows gases produced during fermentation (such as methane) to accumulate inside the bottle, affecting the fermentation process and posing safety hazards; the one-way valve design is inadequate, allowing external oxygen to seep in and disrupt the anaerobic environment.

[0004] Therefore, developing an in vitro fermentation device that can comprehensively and realistically simulate the rumen environment, and is safe, efficient, and precise, is of great significance for precision nutrition research and production practices in ruminants. Summary of the Invention

[0005] This invention aims to solve the technical problems existing in related technologies. To this end, this invention proposes an in vitro fermentation device for simulating the rumen environment of ruminants, to address the problems of poor safety, low uniformity, and limited functionality of existing devices, thereby providing an efficient and precise tool for ruminant nutrition research.

[0006] This invention provides an in vitro fermentation device for simulating the rumen environment of ruminants, comprising: The box body has a digestive chamber in the middle to simulate the internal environment of the rumen; The fermentation bottle is rotatably mounted in the digestive chamber to simulate the peristaltic effect of the rumen; A temperature control module is used to regulate the internal temperature of the digestive chamber; A speed control module is used to drive the fermentation bottle to rotate; The gas management module is used to maintain the anaerobic environment; The fermentation flask array is arranged in the digestion chamber, and the speed control module is equipped with a transmission component for driving the fermentation flasks to rotate synchronously.

[0007] An in vitro fermentation device for simulating the rumen environment of ruminants according to the present invention includes a transmission assembly comprising: A servo motor is located on the outside of the digestive chamber; A drive shaft passes through the side wall of the digestion chamber, with one end of the drive shaft connected to the servo motor and the other end connected to the fermentation flask.

[0008] According to the present invention, an in vitro fermentation device for simulating the rumen environment of ruminants is provided, wherein the transmission assembly further includes a rubber belt disposed within the digestive cavity and connected to the transmission shaft.

[0009] An in vitro fermentation apparatus for simulating the rumen environment of ruminants, according to the present invention, comprises: The bottle body is used to hold the buffer solution and the feed sample to be tested; The bottle cap is equipped with a one-way valve to restrict the gas from flowing from the inside of the bottle to the outside in one direction.

[0010] An in vitro fermentation device for simulating the rumen environment of ruminants according to the present invention includes a temperature control module comprising: A PTC heater is disposed around the outside of the digestive chamber for heating the digestive chamber; An axial flow fan is installed at the top of the digestive chamber to promote uniform heat distribution.

[0011] An in vitro fermentation apparatus for simulating the rumen environment of ruminants according to the present invention further includes a temperature control module comprising: A refrigeration compressor is located at the bottom of the housing and is used to cool the digestive chamber. A temperature probe is installed inside the digestive chamber to monitor the internal temperature of the digestive chamber.

[0012] An in vitro fermentation device for simulating the rumen environment of ruminants according to the present invention includes a gas management module comprising: An air inlet, installed on the side wall of the digestive chamber, is used to introduce nitrogen gas to maintain an anaerobic environment; An exhaust valve, installed on the side wall of the digestive chamber, is used to discharge the gas produced during the fermentation of the feed sample.

[0013] According to the present invention, an in vitro fermentation device for simulating the rumen environment of ruminants is provided, wherein an intelligent control panel is provided on the top of the chamber, and the temperature control module, the speed control module and the gas management module are all electrically connected to the intelligent control panel.

[0014] According to the present invention, an in vitro fermentation device for simulating the rumen environment of ruminants is provided, wherein the intelligent control panel integrates temperature setting, speed adjustment, exhaust program setting, and real-time data monitoring functions.

[0015] According to the present invention, an in vitro fermentation device for simulating the rumen environment of ruminants is provided, wherein the PTC heater adopts a flameless ceramic heating element.

[0016] The above-described one or more technical solutions of this invention have at least one of the following technical effects: The in vitro fermentation device integrates functions such as precise temperature control, anaerobic environment maintenance, mechanical peristalsis simulation, and gas dynamic management to achieve a realistic simulation of the rumen degradation process of feed ingredients or TMR, providing a scientific basis for precise formulation of ruminant diets, optimization of feed formulation, and improvement of animal production performance.

[0017] In addition to the technical problems solved by the present invention, the technical features of the technical solutions constituted by the present invention, and the advantages brought about by the technical features of these technical solutions as described above, other technical features of the present invention and the advantages brought about by these technical features will be further explained in conjunction with the accompanying drawings, or will be learned through the practice of the present invention. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of the present invention or related technologies, the drawings used in the description of the embodiments or related technologies will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the in vitro fermentation apparatus provided in an embodiment of the present invention.

[0020] Figure 2This is a schematic diagram of the structure of a fermentation bottle provided in an embodiment of the present invention.

[0021] Figure 3 This is a schematic diagram of the structure of a bottle cap provided in an embodiment of the present invention.

[0022] Figure 4 This is a front view of an in vitro fermentation apparatus provided in an embodiment of the present invention.

[0023] Figure 5 This is a rear view of an in vitro fermentation apparatus provided in an embodiment of the present invention.

[0024] Figure 6 Left view of the in vitro fermentation apparatus provided in an embodiment of the present invention.

[0025] Figure label: 1. Main switch; 2. Intelligent control panel; 3. Digestion chamber; 4. Drive shaft; 5. Air inlet; 6. Exhaust valve; 7. Drain outlet; 8. Fermentation flask; 9. PTC heater; 10. Refrigeration compressor; 11. Axial flow fan; 12. Temperature probe; 13. Servo motor; 14. Rubber belt; 15. Check valve. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.

[0027] In an embodiment of the present invention, an in vitro fermentation device is described. This in vitro fermentation device is used to simulate the rumen environment of ruminants and to accurately measure the rumen degradation characteristics of feed or total mixed ration (TMR).

[0028] like Figures 1 to 6 As shown, the in vitro fermentation device mainly includes a box, fermentation bottle 8, temperature control module, speed control module and gas management module.

[0029] A digestive chamber 3 is provided in the middle of the box to simulate the internal environment of the rumen. The fermentation bottle 8 is rotatably disposed in the digestive chamber 3 to simulate the peristaltic effect of the rumen.

[0030] The temperature control module is used to regulate the internal temperature of the digestive chamber 3. The temperature control module can regulate the internal temperature of the digestive chamber 3 within a range of 0℃ to 55℃.

[0031] The speed control module is used to drive the fermentation bottle 8 to rotate. The gas management module is used to maintain the anaerobic environment.

[0032] A plurality of fermentation bottles 8 are arrayed within the digestion chamber 3. The rotation speed control module is equipped with a transmission component for driving the plurality of fermentation bottles 8 to rotate synchronously.

[0033] Furthermore, the transmission assembly includes a servo motor 13 and a transmission shaft 4.

[0034] A servo motor 13 is located on the outside of the digestion chamber 3. A drive shaft 4 passes through the side wall of the digestion chamber 3. One end of the drive shaft 4 is connected to the servo motor 13, and the other end of the drive shaft 4 is connected to the fermentation flask 8.

[0035] Furthermore, the transmission assembly also includes a rubber belt 14. The rubber belt 14 is disposed within the digestive chamber 3 and connected to the transmission shaft 4.

[0036] Furthermore, the fermentation bottle 8 includes a bottle body and a bottle cap.

[0037] The bottle body is used to hold the buffer solution and the feed sample to be tested. The bottle cap is equipped with a one-way valve 15 to restrict the one-way flow of gas from the inside of the bottle to the outside. The bottle cap and bottle body have a good seal, and the one-way valve 15 is equipped with a flexible valve plate, which can both release the gas produced by the fermentation of the feed sample to be tested inside the bottle and prevent external oxygen from seeping into the bottle and disrupting the anaerobic environment.

[0038] Specifically, the digestive chamber 3 contains six fermentation bottles 8. A servo motor 13 drives the six fermentation bottles 8 to rotate synchronously via a transmission shaft 4 and a rubber belt 14. The rotation speed is adjustable and the rotation speed of each bottle is consistent, accurately simulating rumen peristalsis.

[0039] The servo motor 13 of the speed control module can precisely adjust the speed. For example, it can simulate the peristaltic frequency of the rumen at 1-2 times / minute. The rubber belt 14 drive ensures that the six fermentation bottles 8 rotate synchronously, and the speed deviation is less than or equal to ±2%.

[0040] In this embodiment, the in vitro fermentation device integrates functions such as precise temperature control, anaerobic environment maintenance, mechanical peristalsis simulation, and gas dynamic management to achieve a realistic simulation of the rumen degradation process of feed ingredients or TMR, providing a scientific basis for precise formulation of ruminant diets, optimization of feed formulation, and improvement of animal production performance.

[0041] Based on the above embodiments, another embodiment of the present invention introduces an in vitro fermentation device for simulating the rumen environment of ruminants.

[0042] The temperature control module includes a PTC heater 9, an axial flow fan 11, a refrigeration compressor 10, and a temperature probe 12.

[0043] The PTC heater 9 uses a flameless ceramic heating element, which avoids the explosion risk of flammable and explosive gases such as methane. The PTC heater 9 is arranged around the outside of the digestive chamber 3 to heat the digestive chamber 3. An axial flow fan 11 is installed on the top of the digestive chamber 3 to promote uniform heat distribution.

[0044] A refrigeration compressor 10 is located at the bottom of the housing and is used to cool the digestion chamber 3.

[0045] Temperature probe 12 is installed inside the digestive chamber 3 to monitor the internal temperature of the digestive chamber 3. It can trigger an alarm when the internal temperature of the digestive chamber 3 is abnormal, thus ensuring the safety of the experimental process.

[0046] Furthermore, the gas management module includes an air inlet 5 and an exhaust valve 6.

[0047] An air inlet 5 is installed on the side wall of the digestion chamber 3 to introduce nitrogen gas to maintain an anaerobic environment. An exhaust valve 6 is installed on the side wall of the digestion chamber 3 to discharge the gas produced during the fermentation of the feed sample.

[0048] Specifically, inlet 5 is used to introduce nitrogen to maintain an anaerobic environment. Exhaust valve 6 is an automatic solenoid valve that can set the exhaust time and frequency to prevent the accumulation of gases such as methane.

[0049] The PTC heater 9 employs a flameless design to prevent explosions caused by contact with methane produced during fermentation. The refrigeration compressor 10 ensures stable operation of the unit even in high-temperature environments (such as summer in Guangdong and Henan). The axial flow fan 11 achieves efficient heat dissipation through low-power, low-noise operation, while ensuring uniform heating of the fermentation bottle 8.

[0050] Specifically, the digestive chamber 3 is equipped with six fermentation bottles 8. The servo motor 13 drives the six fermentation bottles 8 to rotate synchronously through the transmission shaft 4 and the rubber belt 14. The speed is adjustable and ensures that the speed of each fermentation bottle 8 is consistent, so as to achieve the effect of accurately simulating the mechanical peristalsis of the rumen.

[0051] Meanwhile, the axial flow fan 11 ensures uniform heat distribution and guarantees highly consistent fermentation conditions in each fermentation bottle 8, truly reflecting the rumen degradation characteristics of the feed.

[0052] Furthermore, an intelligent control panel 2 is provided on the top of the enclosure. The temperature control module, the speed control module, and the gas management module are all electrically connected to the intelligent control panel 2.

[0053] The intelligent control panel 2 integrates temperature setting, speed adjustment, exhaust program setting, and real-time data monitoring functions. For example, by setting the exhaust program, the action of the exhaust valve 6 can be set to exhaust for 10 seconds every 10 minutes. The real-time data monitoring function can process the internal temperature of the digestive chamber 3, the speed of the servo motor 13, and alarm information. The intelligent control panel 2 also supports human-computer interaction.

[0054] Temperature probe 12 monitors the internal temperature of digestive chamber 3 in real time and feeds it back to intelligent control panel 2, triggering an alarm when an abnormality occurs.

[0055] Specifically, the in vitro fermentation device has a rectangular enclosure. A smart control panel 2 is located on the top of the enclosure. The smart control panel 2 includes a touchscreen display and operation buttons. A main power switch 1 is located on the back of the enclosure.

[0056] The digestion chamber 3 is located in the middle of the enclosure. Inside the digestion chamber 3 are six fermentation bottles 8 arranged in parallel. The fermentation bottles 8 are made of transparent glass. Each fermentation bottle 8 is connected to a servo motor 13 outside the enclosure via a drive shaft 4. The drive shaft 4 drives all the fermentation bottles 8 to rotate synchronously via a rubber belt 14.

[0057] A PTC heater 9 is installed around the exterior of the digestion chamber 3. A refrigeration compressor 10 is installed at the bottom of the chamber. The refrigeration compressor 10 uses refrigerant circulation to cool the digestion chamber 3. An axial flow fan 11 is installed on the side wall of the chamber.

[0058] Three temperature probes 12 are evenly distributed inside the digestive chamber 3 to monitor the internal temperature of the digestive chamber 3 in real time and transmit the data to the intelligent control panel 2.

[0059] An air inlet 5 is located at the top of the digestion chamber 3. This air inlet 5 is connected to a nitrogen cylinder for supplying high-purity nitrogen. An exhaust valve 6 is installed on the side of the digestion chamber 3. The exhaust valve 6 is an automatic solenoid valve, which allows setting the exhaust interval and exhaust duration.

[0060] Each fermentation bottle 8 is capped with a one-way valve 15. The one-way valve 15 has a flexible valve plate that allows gas inside the bottle to escape outwards, while preventing outside gas from entering the bottle in reverse.

[0061] By controlling the rotational speed of the servo motor 13 through the intelligent control panel 2, the rotational speed range of the fermentation bottle 8 can be made to correspond to the frequency of rumen peristalsis. For example, the rotational speed range of the fermentation bottle 8 can be controlled to be 5-20 rpm.

[0062] Using the in vitro fermentation apparatus of this invention, the parameters of each fermentation bottle 8 in the digestion chamber 3 can be obtained, as shown in the table below: Fermentation bottle number 1 2 3 4 5 6 Temperature (°C) 39.0 39.0 39.1 39.0 39.1 39.0 Rotational speed (rpm) 10 10 10 10 10 10 Actual test results show that the servo motor 13 drives the six fermentation bottles 8 to rotate synchronously through the transmission shaft 4 and the rubber belt 14, ensuring that the speed deviation of each fermentation bottle 8 is less than or equal to ±1.5%. The temperature deviation of the six fermentation bottles 8 is less than or equal to 0.3℃, and no oxygen seeps into the fermentation bottles 8.

[0063] In this embodiment, the in vitro fermentation device, through the synergistic action of the PTC heater 9, the refrigeration compressor 10, and the axial flow fan 11, can achieve stable control of the experimental temperature across all regions and throughout the year, from 0℃ to 55℃. By utilizing the nitrogen inlet 5 and the one-way valve 15 installed in the fermentation bottle 8, the anaerobic environment inside the fermentation bottle 8 can be strictly maintained. The intelligent control panel 2 integrates parameter setting and real-time monitoring functions, supports customized exhaust programs, reduces the need for manual intervention, and improves experimental efficiency. It provides reliable technical support for the precise formulation of diets, optimization of feed formulations, and improvement of animal production performance (such as milk production and daily weight gain) in ruminant production, and has broad application prospects.

[0064] Furthermore, in another embodiment of the present invention, a simulated rumen fermentation experiment using an in vitro fermentation apparatus is described.

[0065] The purpose of this experiment was to determine the rumen dry matter degradation rate of fermented coffee grounds feed.

[0066] The experimental procedure is as follows: Turn on the main switch 1, and set the internal temperature of the digestive chamber 3 to 39℃ (simulating the physiological temperature of the rumen) and the rotation speed of the fermentation bottle 8 to 10rpm (simulating rumen peristalsis) through the intelligent control panel 2.

[0067] The PTC heater 9 and axial flow fan 11 were started. After the temperature probe 12 monitored that the internal temperature of the digestive chamber 3 stabilized at 39±0.5℃, equal amounts of buffer solution (simulated rumen fluid) and the feed sample to be tested were added to each of the six fermentation bottles 8. Fermentation bottle 1 (8) was not filled with buffer solution or the feed sample to be tested and served as a blank control.

[0068] Nitrogen gas is continuously introduced through air inlet 5 for 10 minutes to replace the oxygen in fermentation bottle 8, ensuring a strictly anaerobic environment. Furthermore, the exhaust valve 6 is set to exhaust gas for 10 seconds every 5 minutes to prevent methane accumulation.

[0069] After 48 hours of fermentation, the experiment was stopped, and the gas was released through one-way valve 15. Samples were then removed to determine the dry matter residue and calculate the degradation rate. The degradation rates of each fermentation bottle 8 are shown in the table below: Fermentation bottle number 1 2 3 4 5 6 Dry matter degradation rate (%) 71.72 72.02 72.91 71.89 71.32 72.09 The experimental results showed that the dry matter degradation rate data in the six fermentation bottles had high repeatability (RSD<5%), which truly reflected the rumen degradation characteristics of the feed.

[0070] In summary, the in vitro fermentation device of the present invention, through the coordinated operation of multiple modules such as the temperature control module, the speed control module, and the gas management module, comprehensively simulates the key parameters of the rumen environment of ruminants (temperature, anaerobic, peristalsis, and gas balance), solving the problems of poor safety, low uniformity, and single function of existing technologies, and providing an efficient and precise tool for ruminant nutrition research.

[0071] In the description of the embodiments of the present invention, it should be noted that the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the embodiments of the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of the present invention. In addition, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0072] In the description of the embodiments of the present invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in the embodiments of the present invention based on the specific circumstances.

[0073] In embodiments of the present invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "over," and "on top" of the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0074] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms are not limited to the same embodiments or examples. Moreover, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Furthermore, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0075] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An in vitro fermentation device for simulating the rumen environment of ruminants, characterized in that, include: The box body has a digestive chamber (3) in the middle to simulate the internal environment of the rumen; Fermentation bottle (8) is rotatably disposed within the digestive chamber (3) to simulate the peristaltic effect of the rumen; A temperature control module is used to regulate the internal temperature of the digestive chamber (3); A speed control module is used to drive the fermentation bottle (8) to rotate; The gas management module is used to maintain the anaerobic environment; Among them, a plurality of fermentation bottles (8) are arranged in an array within the digestion chamber (3), and the speed control module is provided with a transmission component for driving the plurality of fermentation bottles (8) to rotate synchronously.

2. The in vitro fermentation device for simulating the rumen environment of ruminants according to claim 1, characterized in that, The transmission assembly includes: A servo motor (13) is located on the outside of the digestive chamber (3); A drive shaft (4) passes through the side wall of the digestion chamber (3), one end of the drive shaft (4) is connected to the servo motor (13), and the other end of the drive shaft (4) is connected to the fermentation bottle (8).

3. The in vitro fermentation device for simulating the rumen environment of ruminants according to claim 2, characterized in that, The transmission assembly also includes a rubber belt (14), which is disposed in the digestive chamber (3) and connected to the transmission shaft (4).

4. The in vitro fermentation apparatus for simulating the rumen environment of ruminants according to any one of claims 1 to 3, characterized in that, The fermentation bottle (8) includes: The bottle body is used to hold the buffer solution and the feed sample to be tested; The bottle cap is equipped with a one-way valve (15) to restrict the gas from passing through the bottle from the inside to the outside in one direction.

5. The in vitro fermentation apparatus for simulating the rumen environment of ruminants according to claim 4, characterized in that, The temperature control module includes: PTC heater (9) is arranged around the outside of the digestive chamber (3) for heating the digestive chamber (3); An axial flow fan (11) is installed on top of the digestive chamber (3) to promote uniform heat distribution.

6. The in vitro fermentation apparatus for simulating the rumen environment of ruminants according to claim 5, characterized in that, The temperature control module also includes: A refrigeration compressor (10) is located at the bottom of the housing and is used to cool the digestion chamber (3); A temperature probe (12) is installed inside the digestive chamber (3) to monitor the internal temperature of the digestive chamber (3).

7. The in vitro fermentation apparatus for simulating the rumen environment of ruminants according to claim 6, characterized in that, The gas management module includes: An air inlet (5) is installed on the side wall of the digestive chamber (3) to introduce nitrogen gas to maintain an anaerobic environment; An exhaust valve (6) is installed on the side wall of the digestive chamber (3) to exhaust the gas produced by the fermentation of the feed sample.

8. The in vitro fermentation apparatus for simulating the rumen environment of ruminants according to claim 7, characterized in that, The top of the enclosure is equipped with an intelligent control panel (2), and the temperature control module, the speed control module and the gas management module are all electrically connected to the intelligent control panel (2).

9. The in vitro fermentation apparatus for simulating the rumen environment of ruminants according to claim 8, characterized in that, The intelligent control panel (2) integrates temperature setting, speed adjustment, exhaust program setting and real-time data monitoring functions.

10. The in vitro fermentation apparatus for simulating the rumen environment of ruminants according to claim 9, characterized in that, The PTC heater (9) uses a flameless ceramic heating element.

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