Rapid breeding method of rosa serrulata for ecological restoration in plateau alpine region

Abstract

The invention relates to a rapid breeding method of rosa serrulata for ecological restoration in plateau alpine regions. Comprising the following steps: collecting current-year healthy branches from a plateau area with the altitude of 2000 m or above, trimming the branches into cuttings of 5-10 cm, and sealing and wrapping cuts; the base parts of the cutting slips are sequentially soaked in a 10-15 mg / L naphthylacetic acid solution and a 80-100 mg / L ABT rooting powder solution; cutting the treated cutting slips into a cultivation container, wherein a matrix is formed by mixing soil, sand, perlite, straw, mushroom residues and ceramsite according to the volume ratio of 8: 3: 1: 1: 1: 1; culturing in a greenhouse environment with the relative humidity of 80-90% and the temperature of 25-30 DEG C, and turning to full illumination after 90 days; after 1-2 growing seasons are cultivated, the container seedlings are placed in a plateau greenhouse to be subjected to seedling hardening for 15-30 days, and then the container seedlings with the matrix are transplanted to a plateau planting field. The method solves the problems of insufficient resource development, difficult seed germination, low cuttage survival rate, slow growth and the like of rosa serrulata.

Description

Technical Field

[0001] This invention relates to the field of plant propagation technology, and in particular to a rapid propagation method for Rosa latifolia used in ecological restoration in high-altitude and cold regions. Background Technology

[0002] Rosehipes pubescens is a shrub belonging to the genus Rosa in the family Rosaceae. It is widely distributed in the plateau regions of Tibet, Qinghai, and Sichuan in my country. It possesses strong drought and cold resistance, and can adapt to altitudes of 2000-4400m, making it an excellent plant resource for ecological restoration in high-altitude and cold regions. Due to its well-developed root system and strong adaptability, it plays an important role in windbreak and sand fixation, soil and water conservation, and ecosystem restoration.

[0003] Currently, ecological restoration in high-altitude and cold regions faces challenges such as limited plant species diversity and poor adaptability. *Rosa pubescens*, as a dominant native shrub species, possesses significant ecological restoration value. However, the propagation of *Rosa pubescens* faces numerous difficulties. Seed propagation is limited by factors such as long dormancy periods and low germination rates, while cutting propagation encounters technical bottlenecks such as low survival rates and slow rooting, severely restricting its large-scale application in high-altitude ecological restoration.

[0004] First, existing propagation methods for cuttings are mostly designed for plants in low-altitude areas or other plant species, without considering the physiological characteristics and ecological adaptability of Rosa pubescens in high-altitude and cold regions. Second, the rooting treatment methods in existing technologies are too simplistic to meet the rapid rooting needs of Rosa pubescens in high-altitude environments. Third, the cutting substrate ratio has not been optimized for the characteristics of high-altitude soils, affecting the survival rate and growth rate of the cuttings. Finally, there is a lack of a complete technical system from cuttings to transplanting.

[0005] Therefore, there is an urgent need to develop a rapid propagation method for Rosa pubescens suitable for ecological restoration in high-altitude and cold regions. By optimizing key technical aspects such as cutting collection and pretreatment, root promotion treatment, substrate ratio, environmental control, and hardening and transplanting, the survival rate and growth rate of Rosa pubescens cuttings can be improved, providing sufficient high-quality seedling resources for ecological restoration in high-altitude and cold regions. Summary of the Invention

[0006] The purpose of this invention is to solve the problems of insufficient development of Rosa latifolia resources, difficulty in seed germination, low survival rate of cuttings and slow growth, so as to meet the local ecological restoration needs of high-altitude and cold regions.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: According to one aspect of the present invention, a method for rapid propagation of Rosa sericea for ecological restoration in high-altitude and cold regions is provided, comprising the following steps: collection and pretreatment: collecting healthy current-year branches of Rosa sericea from high-altitude areas above 2000 meters, pruning them into 5-10cm cuttings, and sealing the cut ends with a waterproof material; root-promoting treatment: immersing the base 3-5cm of the cuttings sequentially in a 10-15mg / L naphthaleneacetic acid solution for 12 hours and then in an 80-100mg / L ABT rooting powder solution for 30-40 minutes. Containerized cultivation: The treated cuttings are inserted into cultivation containers filled with a specific substrate, which is a mixture of soil, sand, perlite, straw, mushroom residue and ceramsite in a volume ratio of 8:3:1:1:1:1; Environmental control: Cultivation is carried out in a greenhouse environment with a relative humidity of 80%-90% and a temperature of 25℃-30℃. After 90 days after cutting, the cultivation is switched to full light; Hardening off and transplanting: After 1-2 growing seasons, the container seedlings are placed in a high-altitude greenhouse for hardening off for 15-30 days, and then transplanted with the substrate to the high-altitude planting site.

[0008] Optionally, the soaking sequence of the naphthaleneacetic acid solution and the ABT rooting powder solution can be sequential.

[0009] Optionally, the cultivation container has a bottom diameter of 10-15cm and a height of 15-20cm.

[0010] Optionally, before transplanting, apply a base fertilizer containing organic fertilizer and slow-release NPK compound fertilizer to the bottom of the planting pit.

[0011] Alternatively, during cultivation, water no more than twice a week and apply organic fertilizer.

[0012] Alternatively, during the hardening-off period, the humidity inside the greenhouse can be gradually reduced, and the frequency of watering can be decreased.

[0013] The advantages of this invention are as follows: By combining container cultivation in low-altitude areas with high-altitude transplantation, along with scientific plant growth regulator treatment, specific substrate ratios, and environmental control, the propagation success rate of *Rosa sericea* is significantly improved. Compared with traditional propagation methods, the cutting survival rate of *Rosa sericea* using this method can be increased to over 80%, and the growth rate is accelerated by 30%-50%, effectively solving the problems of difficult seed germination, low cutting survival rate, and slow growth. Simultaneously, the container cultivation method facilitates transportation and transplantation, meeting the practical needs of ecological restoration in high-altitude and cold regions. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 This is a flowchart of the rapid propagation method for Rosa spinulosa described in this invention. Detailed Implementation

[0016] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0017] Example 1 like Figure 1 As shown, a rapid propagation method for Rosa spinulosa used for ecological restoration in high-altitude and cold regions includes the following steps: Step 1: Data Acquisition and Preprocessing Healthy, current-year shoots of *Rosa pubescens* are collected from high-altitude areas above 2000 meters. Vibrant branches are selected and pruned into 5-10cm cuttings. The cut ends are then completely sealed with waterproof bandage to prevent moisture loss. The wrapped cuttings are then placed in sealed plastic bags for storage, facilitating subsequent processing and transportation.

[0018] Step 2: Root-promoting treatment First, soak the base 3-5 cm of the cuttings in a 10-15 mg / L naphthaleneacetic acid (NAA) solution for 12 hours, then soak them in an 80-100 mg / L ABT rooting powder solution for 30-40 minutes. This sequential soaking order significantly improves the rooting rate of the cuttings. *Rosa sericea*, belonging to the genus *Rosa* in the family Rosaceae, is a difficult-to-root woody plant. In addition to pre-treating the cuttings with NAA, treatment with a diluted ABT solution is also necessary.

[0019] Before treating the branches of Rosa laevis, a pretreatment experiment was conducted, with 10 plants in each experiment. Different concentrations of naphthaleneacetic acid (NAA) and soaking times were used to determine the suitable NAA concentration and soaking time for rooting of Rosa laevis. The experimental results are shown in Table 1 below.

[0020] Table 1: Effects of naphthaleneacetic acid concentration and soaking time on rooting of Rosa rugosa

[0021] It was found that naphthaleneacetic acid (NAA) treatment of *Rosa filamentosa* cuttings increased both rooting rate and root number. The overall trend was an initial increase followed by a decrease in both concentrations. Rooting rate and root number tended to stabilize at NAA concentrations of 10-15 mg / L, but decreased with further increases in concentration. At the same concentration, rooting rate generally increased with soaking time, reaching its highest point at a concentration of 10-15 mg / L and a soaking time of 12 hours.

[0022] ABT rooting agent is a plant growth regulator developed by the Chinese Academy of Forestry. It promotes rooting by regulating endogenous plant hormones. Recommended concentrations are shown in Table 2 below.

[0023] Table 2: Recommended Concentration and Soaking Time for ABT Rooting Agent

[0024] Step 3: Containerized Cultivation Prepare cultivation containers with a base diameter of 10-15cm and a height of 15-20cm. Insert one root-promoting *Rosa filamentosa* cutting into each container. Fill the containers with a specific substrate composed of soil, sand, perlite, straw, mushroom residue, and expanded clay pebbles in a volume ratio of 8:3:1:1:1:1. This substrate combination ensures good aeration and drainage while providing adequate nutrients and moisture retention, which is beneficial for rooting and subsequent growth of the cuttings.

[0025] Through matrix ratio experiments, the mixing ratio with the best looseness and air permeability was selected, and the results are shown in Table 3 below.

[0026] Table 3: Effect of different matrix ratios on looseness and air permeability

[0027] It can be seen from the previous substrate ratio test that the main substrate (soil) and the added substrate (sand, perlite, etc.) affect the looseness and air permeability of the mixed substrate. The best mixing ratio with the best looseness and air permeability is selected as 8:3:1:1:1:1.

[0028] Step 4: Environmental Control After propagation, place the containerized seedlings in a greenhouse, maintaining a relative humidity of 80%-90% and a temperature of 25℃-30℃. In the initial stages of propagation, shading is necessary to reduce water loss. After 90 days, gradually remove the shade netting, transitioning to full sunlight to promote photosynthesis. During the cultivation period, water no more than twice a week to avoid waterlogging and rotting of the cuttings. Regularly apply organic fertilizer to provide essential nutrients for growth.

[0029] Step 5: Hardening off and transplanting After the containerized Rosa pubescens seedlings have successfully completed 1-2 growing seasons, they are transported to the plateau for hardening-off treatment before transplanting. The seedlings are placed in a high-altitude greenhouse for 15-30 days, during which time the humidity is gradually reduced and watering frequency is decreased to allow the seedlings to adapt to the high-altitude environment. After hardening-off, planting pits of appropriate size are dug at the planting site. A base fertilizer containing organic fertilizer and slow-release NPK compound fertilizer is applied to the bottom of the planting pit. The seedlings are then transplanted to the planting site with the substrate. After transplanting, a single watering is applied to help the roots establish. Subsequently, the frequency of maintenance is gradually reduced to allow the Rosa pubescens to adapt to the natural environment, achieving successful establishment and growth in the high-altitude, cold region.

[0030] The *Rosa filamentosa* cultivated using the above method exhibits strong adaptability and a high survival rate, enabling it to grow rapidly in high-altitude and cold regions and play a vital role in ecological restoration. Practice has shown that the survival rate of container seedlings cultivated using this method after transplanting can reach over 85%, approximately 30% higher than that of traditional methods. Furthermore, the seedlings grow vigorously and can form a stable vegetation cover after one year, effectively preventing soil erosion and improving the plateau's ecological environment.

[0031] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A rapid propagation method for Rosa spinulosa used in ecological restoration in high-altitude and cold regions, characterized in that, Includes the following steps: Collection and pretreatment: Collect healthy current-year branches of Rosa pubescens from plateau areas above 2000 meters in altitude, prune them into 5-10cm cuttings, and seal the cuts with waterproof material; Rooting treatment: Soak the base 3-5cm of the cuttings in a 10-15mg / L naphthaleneacetic acid solution for 12 hours and then in an 80-100mg / L ABT rooting powder solution for 30-40 minutes. Containerized cultivation: The treated cuttings are inserted into cultivation containers filled with a specific substrate, which is a mixture of soil, sand, perlite, straw, mushroom residue and ceramsite in a volume ratio of 8:3:1:1:1:1; Environmental control: Cultivate in a greenhouse environment with a relative humidity of 80%-90% and a temperature of 25℃-30℃. After 90 days of cutting, switch to full light cultivation. Hardening off and transplanting: After cultivating for 1-2 growing seasons, place the container seedlings in a high-altitude greenhouse for hardening off for 15-30 days, and then transplant them with substrate to the high-altitude planting site.

2. The method according to claim 1, characterized in that, The soaking sequence of the naphthaleneacetic acid solution and the ABT rooting powder solution is sequential.

3. The method according to claim 1, characterized in that, The specifications of the cultivation container are a bottom diameter of 10-15cm and a height of 15-20cm.

4. The method according to claim 1, characterized in that, Before transplanting, apply a base fertilizer containing organic fertilizer and slow-release NPK compound fertilizer to the bottom of the planting pit.

5. The method according to claim 1, characterized in that, During cultivation, water no more than twice a week and apply organic fertilizer.

6. The method according to claim 1, characterized in that, During the hardening-off period, gradually reduce the humidity inside the greenhouse and reduce the frequency of watering.

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