Cultivation method for promoting rapid growth of beet longistamen seedlings

Abstract

The invention relates to a cultivation method for promoting rapid growth of young seedlings of beet longstamen, belongs to the technical field of cultivation, seedling raising and afforestation, and aims to solve the problems that the young seedlings of woody vegetable beet longstamen grow slowly and the output period is too long in the cultivation process. The method mainly comprises the following steps: 1) seed preparation and sowing; 2) timely planting excellent hosts with vegetable value; and 3) maintaining a parasitic relationship and maintaining the seedlings. On the basis of the root semi-parasitic characteristic of the beet longstamen, other woody vegetables with short output periods are selected as hosts to promote the growth of the seedlings of the beet longstamen, the growth vigor of the seedlings of the beet longstamen is improved, the output window period of the beet longstamen can be filled by the output of the hosts, and the economic benefit of unit land area is improved. The method disclosed by the invention is remarkable in effect and eco-friendly, lays a solid foundation for large-scale efficient cultivation of the seedlings of the beet longstamen, and has a wide application prospect and a relatively high popularization value in the field of planting of the beet longstamen.

Description

Technical Field

[0001] This invention belongs to the field of plant seedling and cultivation technology, specifically relating to a cultivation method that promotes the rapid growth of long-stamen beet seedlings by planting superior host plants. Background Technology

[0002] long-stamen beet tree ( Yunnanopilia longistaminea ), also known as beet tree or stem-flowered mountain beet, belongs to the genus *Saccharum* of the family Opiliaceae. Yunnanopilia *Saccharomyces cerevisiae* is a unique monotypic genus of evergreen shrubs or small trees, reaching up to 7 meters in height, mainly distributed in the dry-hot river valleys of Yunnan, where the subtropics transition to the tropics. *Saccharomyces cerevisiae* is a highly regional and distinctive woody vegetable; its tender branches, leaves, inflorescences, and young fruits can be eaten as vegetables. Rich in protein, minerals, and antioxidants, it is an important part of Yunnan's distinctive culinary culture and has a long history of consumption. However, due to over-harvesting and unreasonable development, the wild population of *Saccharomyces cerevisiae* has sharply declined, facing a serious survival crisis. To reduce dependence on wild resources, artificial cultivation has become an inevitable choice. However, under artificial cultivation conditions, *Saccharomyces cerevisiae* seedlings grow extremely slowly, with a long production gap (more than 4 years), becoming a key bottleneck restricting its industrial development.

[0003] Current research on *Saccharum longicornis* mainly focuses on reproductive biology, genetic diversity, systematics, and chemical composition analysis. Cultivation techniques are primarily studied in seedling propagation. While some literature reports on optimizing cultivation substrates and fertilization methods to promote seedling growth, the results have been less than ideal. Slow seedling growth and a long production cycle remain unresolved technical challenges in *Saccharum longicornis* production. The semi-parasitic nature of *Saccharum longicornis* roots has long been overlooked, leading to a lack of understanding of its unique nutrient absorption mechanisms. Traditional cultivation methods for *Saccharum longicornis* are designed based on autotrophic plant models, failing to meet the unique needs of this root-parasitic plant. This is a significant reason for low seedling survival rates, slow growth, long production cycles, and unstable yield and quality under artificial cultivation conditions, severely hindering the industrial development of *Saccharum longicornis* as a distinctive woody vegetable.

[0004] To address the issues of slow seedling growth and long production cycle in the cultivation of long-stamen beet, it is necessary to conduct systematic research on its semi-parasitic root characteristics, analyze its host selection preferences, screen out safe and efficient host plants, innovate cultivation models, and develop seedling care and cultivation methods suitable for the unique biological characteristics of long-stamen beet. Summary of the Invention

[0005] The purpose of this invention is to provide a cultivation method that can promote the rapid growth of long-stamen beet seedlings and shorten the production cycle.

[0006] This method selects mature long-stamen beetroot seeds, promotes germination at the appropriate time to obtain robust seedlings, and intercrops fast-growing woody vegetables as host plants as early as possible during the seedling stage. Through pruning and reasonable fertilization, the parasitic and competitive relationship between long-stamen beetroot and its host is balanced, promoting the rapid growth of long-stamen beetroot seedlings. At the same time, the production cycle is shortened by utilizing the output of fast-growing host plants, thereby improving the economic benefits per unit area of ​​land and solving the problems of slow seedling growth and long production cycle in long-stamen beetroot cultivation.

[0007] This application provides a cultivation method for promoting the rapid growth of long-stamen beet seedlings, comprising the following steps: (1) After cleaning the pericarp of the long-stamen beet fruit, seeds without pulp residue were obtained. The seeds without pulp residue were pretreated and then sown to obtain long-stamen beet seedlings. (2) At the same time as the sowing of long-stamen beet seeds or in the early stage of seedlings, the host plant is one or more woody vegetables suitable for cultivation in the Yuanjiang River Basin where long-stamen beet is distributed; (3) Shade the seedlings during the seedling stage, fertilize and prune after planting the host plant.

[0008] Furthermore, in step (1), the long-stamen beet fruit is harvested from fruits that mature in May and June.

[0009] Furthermore, the pretreatment in step (1) is as follows: soak the seeds in a 2000-fold dilution of 15wt% triadimefon wettable powder for 30 minutes and then rinse them. Soak them in a 0.05wt% gibberellin solution for 2 hours and then rinse them. Spread them out in a shady place to dry for 6-24 hours before sowing.

[0010] Furthermore, in step (2), the early seedling stage refers to the period within 6 months after seed sowing.

[0011] Furthermore, in step (2), the host planted is a seedling or cutting that is 6-12 months old.

[0012] Furthermore, the cultivation distance between the long-stamen beet seedling and the host is 8-15cm, and the cultivation depth is based on the point where the rootstock meets the soil surface.

[0013] Furthermore, in step (2), when the host plant grows and shades the seedlings of the long-stamen beet tree, the host branches are pruned or fixed with supports.

[0014] Furthermore, the fertilizer applied in step (3) is a ternary compound fertilizer with a nitrogen-phosphorus-potassium mass ratio of 20:10:10.

[0015] Beneficial effects According to the cultivation method of the present invention, the plant height, biomass, and net photosynthetic rate of long-stamen beet seedlings can be increased by more than 2 times, and the seedling growth is significantly better than that of traditional cultivation methods. Simultaneously, the host plant can generate economic benefits in the same year of planting, significantly shortening the production cycle compared to the traditional cultivation model that typically takes four years to generate economic benefits. From a food safety perspective, the cultivation method of the present invention uses woody vegetables as hosts, avoiding the impact of toxic or odorous secondary metabolites from the host on the edible safety or flavor of long-stamen beet. In summary, the cultivation method of the present invention effectively promotes seedling growth, increases yield per unit area of ​​land, and ensures the edible quality of long-stamen beet. Attached Figure Description

[0016] Figure 1 The images show the growth status of the long-stamen beet trees in Example 1 (left: planted with wolfberry) and Comparative Example 1 (right: planted with another long-stamen beet tree).

[0017] Figure 2 The images show the growth status of the long-stamen beet trees in Example 2 (left: planted with Caragana korshinskii) and Comparative Example 2 (right: planted with another long-stamen beet tree). Detailed Implementation

[0018] Our research revealed that the long-stamen beet tree possesses root hemiparasitic characteristics, where its root system establishes vascular bundle connections with the host through parasitic organs (haustoria), obtaining nutrients and water from the host. However, because the parasitic process in root hemiparasitic plants occurs underground, and the above-ground parts are no different from ordinary green plants, the root hemiparasitic characteristics of the long-stamen beet tree have long been overlooked, resulting in its unique nutrient absorption mechanism not being fully understood. This invention, based on the root hemiparasitic characteristics and root development features of the long-stamen beet tree (an evergreen shrub or small tree), proposes a host plant for its early seedling stage. By selecting fast-growing, edible woody vegetable plants as host plants, this approach promotes the growth of long-stamen beet tree seedlings while shortening the production gap period, ensuring food safety, and improving planting efficiency.

[0019] The technical solutions in the embodiments of the present invention will be described in detail below, taking wolfberry and caragana as examples of hosts for long-stamen beet seedlings. It should be noted that the details described herein are only some embodiments of the present invention. All other embodiments obtained by anyone without creative effort based on the embodiments of the present invention are within the protection scope of the present invention.

[0020] Example 1 This invention targets the semi-parasitic root characteristics of the long-stamen beet tree by planting host plants to promote its growth. Simultaneously, considering its safety as a vegetable, fast-growing woody vegetables are selected as hosts to ensure that the establishment of the parasitic relationship does not pose a risk to the safety of the long-stamen beet tree for consumption. While promoting the growth of long-stamen beet seedlings, the output of the host plant can shorten the production gap period and improve the economic benefits per unit area of ​​land. The cultivation method mainly includes the following steps: (1) Treatment of long-stamen beet seeds: Collect and process mature long-stamen beet fruits with good maturity (harvested in June-July), clean the fruit peel, disinfect the seed surface and promote germination, and then sow immediately to obtain robust long-stamen beet seedlings; mature long-stamen beet fruits are harvested in May-June each year, specifically characterized by plump fruits and yellow or orange-yellow peel.

[0021] The treatment method for cleaning, surface disinfection, and hormone-induced germination of long-stamen beetroot seeds is as follows: First, after harvesting mature fruits, peel them as soon as possible, rub off the pulp with window screen, wash with water, and dry in a shady place to obtain seeds. For surface disinfection, soak the seeds in a 2000-fold dilution of 15wt% triadimefon wettable powder for 30 minutes, rinse three times with running water, and then soak them in a 0.05wt% gibberellin (GA3) solution for 2 hours. Rinse three more times with running water, and then spread them out in a shady place to dry for 6-24 hours before sowing in perlite or river sand with a moisture content of 10-15wt%. When sowing in seedling trays, spread a 6-8cm deep layer of perlite in the tray, place the long-stamen beetroot seeds horizontally on the perlite with a spacing of 5cm, and cover the seeds with a 4-5cm layer of perlite. Water until the perlite is completely saturated. At transplanting, the long-stamen beetroot seedlings should be 6 months old, with a hypocotyl elongation of 8-10cm and no lateral roots. Transplanting within the specified timeframe causes minimal disturbance to the root system.

[0022] (2) Selection and planting of host plants: At the same time as the seeds of *Saccharum longicornis* are sown or in the early seedling stage (within 6 months after seed sowing), superior host plants are planted to establish a parasitic relationship between *Saccharum longicornis* and the host plants, so as to promote the growth of *Saccharum longicornis* seedlings. The selection criteria for superior host plants are based on a comprehensive consideration of factors such as food safety, material availability, plant ecological adaptability, product market acceptance and yield, and are one of the woody vegetables suitable for cultivation in the Yuanjiang River Basin, such as wolfberry and *Caragana korshinskii*, which are distributed in the area of ​​*Saccharum longicornis*.

[0023] The host plant should be seedlings or cuttings with a root system that are 6-12 months old and well-developed. Before transplanting, the vigorous above-ground parts and excessively long or damaged roots of the host plant should be pruned appropriately. When planting long-stamen beet seeds or seedlings under 3 months old with host seedlings or cuttings after germination promotion treatment, the distance between the long-stamen beet seeds or seedlings and the host plant should be 8-15 cm. The sowing depth of long-stamen beet seeds should be 3-5 cm from the soil surface, and the transplanting depth of long-stamen beet seedlings should be such that the root-stem junction is level with the substrate surface.

[0024] This embodiment selects the woody vegetable wolfberry ( Lycium chinense As the host plant for the long-stamen beet tree, cuttings with a height of 20cm and 5-10 adventitious roots were used at 3 months old. Before transplanting, the above-ground parts of the wolfberry cuttings were pruned, removing overly dense branches and leaves, as well as diseased and dead branches, to reduce water evaporation and nutrient consumption, and to prevent the plants from wilting due to water supply and demand imbalance after transplanting. At the same time, excessively long and damaged root segments were appropriately pruned to promote the sprouting of new roots and prevent the roots from tangling and knotting in the planting hole during transplanting, ensuring that the host plant can quickly adapt to the new environment after transplanting.

[0025] The timing of host plant selection is based on the root development characteristics of *Saccharum longistylum*, the initial time of parasitic organ (haustorium) formation, and the timing of nutrient consumption from seed storage after seedling emergence. *Saccharum longistylum* seedlings produce few lateral roots for a considerable period after emergence, while haustorium formation primarily occurs on these lateral roots. Therefore, promoting early lateral root development is crucial for establishing a parasitic relationship between *Saccharum longistylum* and its host. Induced by host plant root secretions, *Saccharum longistylum* seedlings gradually develop lateral roots around six months after emergence, followed by haustorium formation. Planting a host plant before this point facilitates early establishment of the parasitic relationship and promotes seedling development. *Saccharum longistylum* itself has weak root absorption capacity and low photosynthetic efficiency; planting a host plant before its seed nutrient stores are depleted promotes seedling development. It is important to note that although *Saccharum longistylum* can obtain nutrients from its host, biomass accumulation still depends on its own photosynthetic products. Because the above-ground parts of the host plant compete with the long-stamen beet tree for light resources, and the long-stamen beet tree grows slowly in its seedling stage, the planting distance needs to be considered when planting the host plant. It is necessary to ensure that the roots of the long-stamen beet tree and the host plant are in full contact, while avoiding excessive competition for light resources between the host plant and the long-stamen beet tree.

[0026] (3) Planting conditions: The planting environment should be selected with an average annual temperature of 13℃-25℃. The winter temperature should not be lower than 5℃. Cultivation should be carried out in greenhouses or polytunnels with sufficient light and good ventilation. The aim is to provide suitable light, temperature and ventilation conditions for the long-stamen beet tree and the host. Shading nets should be used to provide shade at noon in the early stage of transplanting to avoid direct sunlight.

[0027] This cultivation was carried out in the greenhouse of the Kunming Institute of Botany, located at 25°13′55″N, 102°74′53″E, at an altitude of 1990 meters, with an average annual temperature of 14.7℃. In daily management, we ensured good ventilation in the greenhouse at all times, while also taking shading measures to prevent the plants from being exposed to direct sunlight.

[0028] The cultivation substrate used is Seeding peat moss, with a particle size of 0-10 mm and a pH of 5.5-6.0. This substrate has good water retention and aeration, providing a favorable environment for the growth of both the long-stamen beet tree and the host plant's root system. It should be prepared to the appropriate moisture level in advance. After transplanting, water thoroughly and slowly, evenly, until the substrate is fully moistened.

[0029] The cultivation container should be a gallon pot with a mouth diameter of not less than 30cm, a bottom diameter of not less than 20cm, and a depth of not less than 25cm. The size of the gallon pot is designed to meet the growth space requirements of the main root of the long-stamen beet tree, while accommodating the co-growth of the long-stamen beet tree and the host.

[0030] In this embodiment, a rectangular root box with a length of 25cm, a width of 5cm, and a height of 30cm was selected as the cultivation container to facilitate observation of the root development of the long-stamen beet tree.

[0031] The cultivation setup involves planting one long-stamen beetroot and one goji berry plant per pot, with a distance of 8-10 cm between them. The long-stamen beetroot is planted at a depth where the root-stem junction is level with the substrate surface; the goji berry is planted at a depth of 10 cm, ensuring that the roots are completely covered by the substrate and the plant does not fall over. Long-stamen beetroot seeds and the selected host are planted simultaneously, with the planting spacing and depth carefully planned to allow ample space for root growth and ensure a stable root connection. Within this distance, the roots of the long-stamen beetroot and the host can come into contact with each other quickly, thus rapidly establishing a stable parasitic relationship. This provides favorable conditions for their subsequent synergistic growth and effectively promotes the rapid growth of the long-stamen beetroot during the seedling stage.

[0032] The planting depth of the host plant should be flexibly determined according to the specific species of the host plant: for most host plants with a relatively obvious root-stem junction, the planting depth should be controlled so that the root-stem junction is level with the substrate surface; while for host plants with an indistinct root-stem junction, in order to ensure their normal growth, the substrate should completely cover the root system, while ensuring that the plant remains upright and does not fall over during the growth process.

[0033] (3) Nutrient management: After the long-stamen beet tree is planted with a host, it should be fertilized properly and nutrient solution or compound fertilizer should be applied regularly.

[0034] This embodiment uses Long Ashton nutrient solution applied once a week, at a dosage of 100ml per pot. First, prepare separate Long Ashton nutrient solution stock solutions. For each macro-element stock solution, add 43.5g potassium sulfate, 92.4g magnesium sulfate heptahydrate, 111g calcium chloride, 264.3g ammonium sulfate, and 170g sodium nitrate or 47.2g disodium hydrogen phosphate per liter of water. For the micro-element mixed stock solution, add 1.81g manganese chloride tetrahydrate, 0.50g zinc sulfate heptahydrate, 0.08g copper sulfate pentahydrate, 0.025g sodium molybdate dihydrate, and 2.86g boric acid per liter of water. For the iron salt stock solution, add 0.4g sodium ethylenediaminetetraacetate iron per liter of water. Next, the applied nutrient solution was prepared by adding 8 ml of potassium sulfate mother liquor, 4 ml of magnesium sulfate heptahydrate mother liquor, 4 ml of calcium chloride mother liquor, 1 ml of ammonium sulfate mother liquor, 1 ml of iron salt mother liquor, 2 ml of sodium nitrate mother liquor, 4 ml of disodium hydrogen phosphate mother liquor, and 1 ml of trace element mixed mother liquor per liter of water.

[0035] (4) Maintenance and management: From the sowing of long-stamen beet seeds to the early seedling stage and the early stage of host transplanting, shade nets should be used to avoid direct sunlight.

[0036] Prune and shape excessively growing goji berries to prevent them from negatively impacting the seedlings of long-stamen beet trees. Considering the characteristics of goji berries, such as not being able to stand upright and having numerous stems that grow prostrate, when pruning and shaping, select 3-5 evenly distributed and vigorous main branches and cut off the rest of the branches to establish the trunk. After establishing the trunk, shorten the main branches at 25-35cm to promote the growth of lateral branches. Regularly prune overly dense branches, vigorous branches, weak branches, diseased and pest-infested branches, and dead branches. At the same time, use methods such as branch pulling and branch propping to optimize the plant's growth.

[0037] (5) Pest and disease control: Common pests and diseases include aphids, scale insects, spider mites, and mildew. A comprehensive approach combining physical and chemical methods is used for control. Physical methods include using yellow and blue sticky traps and mechanical wiping with 75% alcohol. Chemical control methods involve targeted treatment with pesticides such as imidacloprid, acetamiprid, and chlorpyrifos. Regular monitoring of the growth of the long-stamen beet trees, regular weeding and soil loosening, pest and disease control, and pruning of overly vigorous host plants ensure the healthy growth of the long-stamen beet trees with the assistance of the host plant.

[0038] (6) Data Collection: Twenty months after planting, plant height was measured using a soft measuring tape, stem base was measured using a vernier caliper, and leaf count was recorded. The net photosynthetic rate of the long-stamen beet trees was determined using a Li 6400 photosynthesis system. Measurements were taken from 9:00-11:00 AM and 2:00-5:00 PM, selecting leaves of uniform age and good growth condition. The light intensity was fixed at 1400 μmol / m². -2 s -1 The CO2 concentration in the sample chamber was 400 μmol. -1The gas flow rate in the sample chamber is 300 μmol / s. -1 The air temperature in the leaf chamber was room temperature; the relative chlorophyll content of the leaves was measured using a SPAD-502PLUS portable chlorophyll meter; the long-stamen beet tree was horizontally cut from the base of the stem using pruning shears to divide it into above-ground and underground parts. The underground parts were removed from the substrate, cleaned, and each part was placed in an envelope. After drying at 75°C to constant weight, the dry weight was measured.

[0039] Comparative Example 1 The comparative example was completed strictly in accordance with the example, the only difference being that in the example, one long-stamen beet tree and one host plant were cultivated together, while in the comparative example, two long-stamen beet trees were cultivated together.

[0040] Nonparametric test results show that the experimental data outperformed the comparative data across all statistical dimensions, and the significance of each indicator is [not specified]. P All values ​​were less than 0.05 (Table 1), indicating statistical significance. This demonstrates that after planting wolfberry as a host, the long-stamen beet tree established a good parasitic relationship with it, and the nutrients provided by the host significantly promoted the growth of the long-stamen beet tree. Figure 1 This method effectively improved various growth indicators of *Saccharum longicornis* seedlings. The increased number of leaves in *Saccharum longicornis* seedlings led to a larger photosynthetic area, higher chlorophyll content, and increased net photosynthetic rate, thereby enhancing photosynthetic capacity and accelerating carbohydrate synthesis and accumulation. Consequently, their growth and development were more vigorous, with significant increases in plant height, stem base diameter, and biomass. This demonstrates that the appropriate host plant has a significant effect on increasing the yield of the woody vegetable *Saccharum longicornis*.

[0041] Table 1. Effects of planting wolfberry as a host on long-stamen beet seedlings Note: Bold black text in the table indicates that the experimental factor has a significant impact on the experimental index. P <0.05) or highly significant effect ( P <0.01).

[0042] Example 2 The treatment, cultivation substrate, cultivation setup, maintenance management, and data collection steps for long-stamen beet trees are the same as in Example 1. The difference between Example 2 and Example 1 is: 1) Age of long-stamen beet tree seedlings: In this example, the long-stamen beet trees are the same batch of seedlings as in Example 1, and the seedlings are 1 year old at the time of planting, with lateral roots.

[0043] 2) Host selection: In this embodiment, Caragana is selected as the host of Beetroot. One Beetroot and one Caragana are planted together in each cultivation pot.

[0044] 3) Planting environment selection: The planting area is Lancang Lahu Autonomous County, whose climate is similar to the native environment of the long-stamen beet tree. The specific experimental cultivation site is a greenhouse in Donghui Town. It is located at 22°26′19″N, 99°47′52″E, at an altitude of 1350 meters, with an average annual temperature of 15-28℃.

[0045] 4) Cultivation container: Select a gallon pot with a bottom diameter of 21cm, a mouth diameter of 33cm, and a depth of 28cm as the cultivation container.

[0046] 5) Nutrient Management: In this embodiment, a balanced water-soluble fertilizer containing macronutrients is applied every two weeks, with a nitrogen, phosphorus, and potassium mass ratio of 20:10:10, and micronutrients are also added. Apply the fertilizer according to the dilution ratio and dosage specified in the product instructions to ensure sufficient nutrient supply to the beetroot and goji berry trees.

[0047] Comparative Example 2 The comparative example was completed strictly according to Example 2, the only difference being that in Example 2, one long-stamen beet tree and one host plant were cultivated together, while in this comparative example, two long-stamen beet trees were cultivated together.

[0048] Table 2. Effects of planting Caragana as a host plant on seedlings of Beetroot in Lancang area Note: Bold black text in the table indicates that the experimental factor has a significant impact on the experimental index. P <0.05) or highly significant effect ( P <0.01).

[0049] Experimental data show that, under the cultivation conditions set up in Donghui Town, Lancang County, compared with the control group where the host plant Caragana was planted, all growth indicators of the long-stamen beet were improved to a certain extent in the example of planting Caragana as a host. Figure 2 Independent samples were used. t Statistical analysis revealed significant differences in plant height, total leaf area, total root length, and total root surface area between the *Caragana sinica* and the control group in the study (Table 2). Plant dry weight and stem base diameter also showed varying degrees of improvement. This result confirms that planting *Caragana sinica* can effectively promote the growth of *Caragana sinica* seedlings and has a positive effect on improving many growth indicators of *Caragana sinica* seedlings.

[0050] The various embodiments of this application have been described above. These descriptions are exemplary and not exhaustive, nor are they limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen to best explain the principles, practical application, or improvement of the technology in the market, or to enable others skilled in the art to understand the embodiments disclosed herein.

Claims

1. A cultivation method for promoting rapid growth of long-stamen beet seedlings, characterized in that, Includes the following steps: (1) After cleaning the pericarp of the long-stamen beet fruit, seeds without pulp residue are obtained. The seeds without pulp residue are pretreated and then sown to obtain long-stamen beet seedlings. (2) At the same time as the sowing of long-stamen beet seeds or in the early stage of seedlings, the host plant is one or more woody vegetables suitable for cultivation in the Yuanjiang River Basin where long-stamen beet is distributed; (3) Shade the seedlings during the seedling stage, fertilize and prune after planting the host plant.

2. The cultivation method according to claim 1, characterized in that, In step (1), the long-stamen beet fruit is harvested from fruits that mature in May and June.

3. The cultivation method according to claim 1, characterized in that, The pretreatment in step (1) is as follows: soak the seeds in a 2000-fold dilution of 15wt% triadimefon wettable powder for 30 minutes and then rinse them. Soak them in a 0.05wt% gibberellin solution for 2 hours and then rinse them. Spread them out in a shady place to dry for 6-24 hours before sowing.

4. The cultivation method according to claim 1, characterized in that, In step (2), the early seedling stage refers to the period within 6 months after seed sowing.

5. The cultivation method according to claim 1, characterized in that, In step (2), the host planted is a seedling or cutting that is 6-12 months old.

6. The cultivation method according to claim 1, characterized in that, The cultivation distance between the long-stamen beet seedlings and the host is 8-15cm, and the cultivation depth is based on the point where the rootstock junction is level with the soil surface.

7. The cultivation method according to claim 1, characterized in that, In step (2), when the host plant grows and shades the seedlings of the long-stamen beet tree, the host branches are pruned or fixed with supports.

8. The cultivation method according to claim 1, characterized in that, The fertilizer applied in step (3) is a ternary compound fertilizer with a nitrogen-phosphorus-potassium mass ratio of 20:10:10.

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