Preservation method of radix peucedani seminal roots

Abstract

The invention relates to a peucedanum praeruptorum seminal root preservation method which comprises the following steps: S1, pretreatment: selecting healthy peucedanum praeruptorum seminal roots, performing surface disinfection on the peucedanum praeruptorum seminal roots, and then standing until the surfaces are dry; the dried seminal roots and a filling medium with the humidity of 15%-22% are alternately placed into a breathable container in a layered mode, at most three layers of seminal roots are placed, and every two adjacent layers of seminal roots are physically isolated through the filling medium; s3, storage: the container filled with the seminal roots is placed in a dark and ventilated storage environment for storage, the relative air humidity of the storage environment is controlled to be 30%-50%, the temperature is controlled to be 1-13 DEG C, and the storage time can reach 60 days or longer.

Description

Technical Field

[0001] This application relates to the field of Chinese medicinal herb cultivation technology, and more specifically, to a method for preserving the root of Peucedanum praeruptorum. Background Technology

[0002] As a traditional Chinese medicinal herb, *Peucedanum praeruptorum* primarily relies on root segments for propagation in agricultural production. The roots are typically harvested in winter and need to be stored for an extended period until planting the following spring; this process is crucial for maintaining the physiological activity of the roots. However, current preservation practices generally employ extensive management methods, such as direct sand storage or cellar stacking, which have significant drawbacks. Due to the lack of precise control over environmental parameters, the roots frequently encounter humidity imbalances during storage: excessively high humidity easily leads to the formation of a condensation film on the root surface, creating a breeding ground for mold and causing mildew and rot; excessively low humidity results in excessive water loss from the roots, causing tissue shrinkage and decreased vitality. Simultaneously, temperature fluctuations are difficult to control. When temperatures are too high, the roots are prone to premature budding, consuming limited nutrient reserves and weakening the survival potential for subsequent planting; excessively low temperatures may induce frost damage, destroying cell structure. Furthermore, traditional stacking methods often result in excessively thick stacks with poor internal ventilation, leading to the continuous accumulation of heat and carbon dioxide, which not only accelerates metabolic disorders in the roots but also exacerbates the risk of localized heating and rot. These factors combine to cause a sharp deterioration in the physiological state of the rootstocks during storage, resulting in reduced germination rates, weakened disease resistance, and even complete loss of cultivation value, severely restricting the stable supply and profitability of medicinal herbs. Therefore, there is an urgent need for a preservation solution that can systematically address environmental control challenges while also ensuring ease of operation and economic feasibility.

[0003] To address the aforementioned issues, existing technologies urgently need improvement.

[0004] Invention Content The purpose of this application is to provide a method for preserving the root of Peucedanum praeruptorum, which can effectively maintain the physiological activity of the root, prevent mold, shrinkage and premature germination during storage, and improve the preservation quality and germination rate of subsequent planting.

[0005] This application provides a method for preserving the root of Peucedanum praeruptorum, the technical solution of which is as follows: Includes the following steps: S1. Pretreatment: Select healthy Peucedanum praeruptorum roots, disinfect their surface, and then let them stand until the surface is dry; S2. Packing: The dried seed roots and filling medium with a moisture content of 15%-22% are alternately layered into a breathable container. The seed roots can be placed in a maximum of three layers, and the seed roots in adjacent layers are physically isolated by the filling medium. S3. Storage: Place the container containing the seed roots in a dark, well-ventilated storage environment. Control the relative humidity of the storage environment to 30%-50% and the temperature to 1℃-13℃. The storage time can be more than 60 days.

[0006] Furthermore, this application also proposes that, in step S1, surface disinfection includes spraying a disinfectant using an atomizing device, the disinfectant being selected from at least one of 75% ethanol, 0.1% potassium permanganate solution, 800 times dilution of 50% carbendazim wettable powder, 1500 times dilution of 25% azoxystrobin suspension, 1% allicin extract, or 500 times dilution of 10 billion CFU / g Bacillus subtilis wettable powder; and / or, before or after spraying the disinfectant, it also includes a physical disinfection step of irradiation with ultraviolet light or fumigation with low-concentration ozone.

[0007] Furthermore, this application also proposes that, in step S2, the filling medium includes a base medium and functional additives; The base medium is fine river sand or sieved fine garden soil; The functional additives are selected from at least one of vermiculite, perlite, wood ash, activated carbon powder, and well-rotted and dried organic fertilizer granules.

[0008] Furthermore, this application also proposes that the filling medium, by volume percentage, includes: 70%-80% base medium, 10%-15% vermiculite or perlite, and 5%-10% wood ash or activated carbon powder.

[0009] Furthermore, this application also proposes that in step S2, the breathable container is a plastic turnover basket with breathable holes on the side walls and bottom; when filling the basket, first lay a layer of filling medium with a thickness of 3-5 cm at the bottom of the basket, then lay a layer of seed roots with a spacing of ≥1 cm, then cover it with a layer of filling medium with a thickness of 2-3 cm, repeat the above operation for up to three layers, and finally cover the top layer with a layer of filling medium with a thickness of 4-7 cm.

[0010] Furthermore, this application also proposes that, in step S3, the temperature of the storage environment is controlled at 2℃-10℃ and the relative humidity of the air is controlled at 35%-45%.

[0011] Furthermore, this application also proposes that in step S3, the storage environment is equipped with temperature and humidity control equipment, and environmental parameters are monitored by temperature and humidity sensors, and the temperature and humidity control equipment is controlled in response to maintain the environmental temperature and humidity within the set range.

[0012] Furthermore, this application also proposes that the temperature and humidity control device includes one or more of an air conditioner, a dehumidifier, a humidifier, and a ventilation fan; the method further includes: automatically starting the ventilation fan for ventilation when the CO2 concentration in the environment exceeds a set threshold.

[0013] Furthermore, this application also proposes that during the storage period in step S3, the humidity of the filling medium in the container be checked periodically, and when the humidity of the medium is lower than the lower limit, it be replenished with moisture by atomization.

[0014] Furthermore, this application also proposes that, before the seed roots are removed for planting after the storage period expires in step S3, a root awakening step is also included: placing the seed roots together with the container or after removal at 10℃-15℃ under diffused light for 3-10 days.

[0015] As can be seen from the above, the method for preserving Peucedanum praeruptorum seed roots provided in this application, by controlling the humidity and temperature of the storage environment and employing a layered crate packing and regular inspection mechanism, ensures that the seed roots remain in good condition during storage. This effectively maintains the physiological activity of the Peucedanum praeruptorum seed roots, prevents mold, shrinkage, and premature germination during storage, and improves the preservation quality and germination rate of subsequent planting. (See attached figures for details.) Figure 1 This is a flowchart illustrating the method for preserving the root of Peucedanum praeruptorum in this application embodiment.

[0016] Figure 2 This is a sample image of the root of *Peucedanum praeruptorum* after processing in step S1 of this embodiment.

[0017] Figure 3 This is a diagram illustrating the framing preparation process in step S2 of this application embodiment.

[0018] Figure 4 This is a sample image after framing in step S2 of this application embodiment. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0020] The following disclosure provides numerous different embodiments or examples for implementing various structures of the invention. To simplify the disclosure, specific examples of components and arrangements are described below. These are merely examples and are not intended to limit the scope of the invention. Furthermore, reference numerals and / or letters may be repeated in different examples. Such repetition is for simplification and clarity and does not in itself indicate a relationship between the various embodiments and / or arrangements discussed.

[0021] Traditional methods for preserving Peucedanum praeruptorum seeds often involve sand storage or cellar stacking. However, improper control of humidity and temperature during storage frequently leads to root rot, mold growth, or premature sprouting, resulting in decreased root viability or even rendering the seeds unusable. These methods lack precise control over humidity and ventilation, making them prone to mold growth. Furthermore, excessively thick stacking can cause internal heating and rotting, making it difficult to maximize the preservation of the root's physiological activity.

[0022] In this regard, such as Figure 1-4 As shown, this application proposes a method for preserving the roots of *Peucedanum praeruptorum*. Through pretreatment, basketing, and storage, the roots are surface-sterilized and dried. They are then alternately layered with a filling medium of specific humidity and placed in a breathable container. The container is then placed in a light-proof, well-ventilated storage environment with precisely controlled temperature and humidity. This effectively solves the problems of root rot, mold, and premature germination in existing technologies, achieving long-term preservation and maintaining its physiological activity. Specifically, the method includes the following steps: S1. Pretreatment: Select healthy Peucedanum praeruptorum roots, disinfect their surface, and then let them stand until the surface is dry; S2. Packing: The dried seed roots and filling medium with a moisture content of 15%-22% are alternately layered into a breathable container. The seed roots can be placed in a maximum of three layers, and the seed roots in adjacent layers are physically isolated by the filling medium. S3. Storage: Place the container containing the seed roots in a dark, well-ventilated storage environment. Control the relative humidity of the storage environment to 30%-50% and the temperature to 1℃-13℃. The storage time can be more than 60 days.

[0023] For ease of understanding, the following explains some key terms in this embodiment: Peucedanum rootstock refers to the underground rhizome used for propagating Peucedanum. Its health directly affects the preservation effect and subsequent planting survival rate. Root segments that are free from pests and diseases, have no mechanical damage, are plump, and have intact buds are typically selected.

[0024] Surface disinfection refers to cleaning and sterilizing the surface of the root of Angelica dahurica to remove or inhibit pathogenic microorganisms such as bacteria and fungal spores attached to the root surface, thereby reducing the risk of rotting and mold during storage.

[0025] The filling medium refers to the substance used to alternately layer with the roots of *Angelica dahurica* during the basketing process. Its main function is to provide a stable microenvironment, regulate the humidity around the roots, prevent the roots from losing water or becoming too wet, and provide physical isolation to reduce direct contact between roots and the spread of diseases.

[0026] A breathable container is a container with good air permeability used to hold the roots of Angelica dahurica and the filling medium. Its air permeability helps the air to circulate inside the container, avoiding excessive local humidity and the accumulation of harmful gases, thus providing a relatively stable storage environment for the roots.

[0027] The storage environment refers to the external environment in which the roots of Peucedanum praeruptorum are stored. The temperature, humidity, light, and ventilation conditions of this environment need to be precisely controlled to slow down the physiological metabolic activities of the roots, inhibit the growth and reproduction of pathogenic microorganisms, prevent the roots from sprouting or rotting prematurely, and thus extend the shelf life.

[0028] The specific implementation process of the method for preserving the root of Peucedanum praeruptorum in this embodiment is as follows: In the pretreatment step S1, healthy Peucedanum praeruptorum roots must first be selected. For example, the roots can be visually inspected to select those that are plump, without obvious lesions, mechanical damage, or signs of insect infestation. Subsequently, the selected roots are surface-sterilized. Surface sterilization can be achieved in various ways, such as immersing the roots in a diluted disinfectant solution or applying the disinfectant evenly to the root surface using a spray. After sterilization, the roots are left to stand and allow their surface to dry naturally, avoiding any residual moisture.

[0029] In step S2, the pre-treated and dried rootstocks are alternately layered with the filling medium and placed into a breathable container. The moisture content of the filling medium needs to be controlled within the range of 15%-22%, which can be adjusted by pre-weighing and mixing the moisture. When filling the container, a layer of filling medium can be laid at the bottom of the container first, then a layer of rootstocks can be laid flat, and then another layer of filling medium can be covered, and so on. To avoid excessive stacking of rootstocks, which could lead to internal heating and damage, the number of layers of rootstocks is limited to a maximum of three. In addition, adjacent layers of rootstocks are physically isolated by the filling medium to ensure that the rootstocks do not come into direct contact.

[0030] In step S3, the containers filled with the root seeds are placed in a specific storage environment. This environment needs to be dark and well-ventilated; for example, the containers can be placed in a room or warehouse without direct sunlight and with natural air circulation. Simultaneously, the relative humidity of the storage environment is controlled, maintaining it within the range of 30%-50%. The temperature of the storage environment also needs to be precisely controlled, maintaining it within the range of 1℃ to 13℃. Through the synergistic effect of these environmental conditions, the root seeds of *Peucedanum praeruptorum* can be preserved for more than 60 days.

[0031] This method involves rigorous pretreatment of the roots of *Peucedanum praeruptorum*, including selection of healthy roots, surface disinfection, and drying, to control disease risk at the source. Subsequently, the roots are layered and packed in baskets using a filling medium with specific humidity, limiting the number of stacked layers to effectively maintain a stable microenvironment, promote air circulation, and physically isolate disease transmission. Finally, the roots are stored in a dark, well-ventilated environment with precisely controlled temperature and humidity, significantly slowing down root metabolism, inhibiting rot, mold, and premature germination, thus achieving long-term preservation of *Peucedanum praeruptorum* roots and maximizing their physiological activity.

[0032] In some of the embodiments described above in this application, surface disinfection is proposed to reduce pathogen contamination on the surface of seed roots. However, in its implementation, the disinfection method may not be comprehensive enough, resulting in incomplete disinfection and increasing the risk of rot and mold during storage.

[0033] In this regard, this application further proposes that in step S1, the surface disinfection includes spraying a disinfectant using an atomizing device, wherein the disinfectant is selected from at least one of 75% ethanol, 0.1% potassium permanganate solution, 800 times dilution of 50% carbendazim wettable powder, 1500 times dilution of 25% azoxystrobin suspension, 1% allicin extract, or 500 times dilution of 10 billion CFU / g Bacillus subtilis wettable powder; and / or, before or after spraying the disinfectant, it further includes a physical disinfection step of irradiation with ultraviolet light or fumigation with low concentration ozone.

[0034] Specifically, atomizing devices are used to spray disinfectant. These devices disperse the liquid disinfectant into extremely fine droplets, allowing it to adhere evenly and meticulously to all surfaces of the rootstock, including tiny crevices and depressions, thereby improving the coverage and penetration of the disinfectant. For example, handheld electric atomizers can be used, allowing operators to spray each stacked or laid-out rootstock individually, suitable for small-batch processing. Alternatively, automated spray lines can be used, transporting the rootstock via conveyor belts with fixed or movable atomizing nozzle arrays above and / or to the sides, enabling continuous and efficient large-scale disinfection operations. The disinfectant is selected to provide broad-spectrum bactericidal and bacteriostatic effects to combat various pathogenic microorganisms, including bacteria and fungi, that may be encountered during the storage of *Angelica dahurica* rootstock. By selecting disinfectants with different mechanisms of action, the effectiveness of disinfection can be improved and the drug resistance of pathogens can be reduced. For example, for common fungal diseases, chemical fungicides such as 50% carbendazim wettable powder at 800 times dilution or 25% azoxystrobin suspension at 1500 times dilution can be sprayed to quickly and effectively kill or inhibit fungal growth. If environmental protection and biological control are emphasized, or if bacterial inhibition is required, 10 billion CFU / g Bacillus subtilis wettable powder at 500 times dilution or 1% allicin extract can be used, utilizing their biological antagonistic effects or natural antibacterial components to reduce pathogens. In addition, physical disinfection steps such as ultraviolet lamp irradiation or low-concentration ozone fumigation can be used. Physical disinfection, as a supplement or alternative to chemical disinfection, can kill or inhibit microorganisms through non-chemical means, reducing chemical residues and filling in any blind spots that may exist in chemical disinfection, further improving the thoroughness of disinfection. For example, in a dedicated ultraviolet disinfection box or channel, the roots are exposed to ultraviolet light of a specific wavelength. Ultraviolet light can destroy the DNA structure of microorganisms, rendering them unable to reproduce. The irradiation time can be adjusted according to the UV lamp intensity and the root surface area. For example, under a UV intensity of 200 μW / cm², irradiation can be performed for 15-30 minutes. Alternatively, the roots can be placed in a sealed fumigation chamber and fumigated using a low-concentration ozone gas generated by an ozone generator. Ozone has strong oxidizing properties and can destroy the cell walls and cell membranes of microorganisms, thereby achieving a bactericidal effect. The fumigation concentration is usually controlled at 0.5-2 ppm, and the fumigation time is 30-90 minutes, after which ventilation is required.

[0035] The above-mentioned technical solution employs an atomizing device to spray disinfectant, ensuring uniform coverage of the root surface and avoiding localized disinfection failures caused by uneven spraying in traditional methods, thus significantly improving overall disinfection efficiency. Simultaneously, the diverse selection of disinfectants, including chemical and biological agents, provides flexible and broad-spectrum bactericidal effects against different types of pathogens, enhancing the breadth of disease control. Furthermore, optional physical disinfection steps such as ultraviolet lamp irradiation or low-concentration ozone fumigation provide supplementary treatment on top of chemical disinfection, effectively eliminating potential disinfection blind spots and residual pathogens, further reducing the risk of chemical residues. This ensures comprehensive and thorough disinfection of the rootstock, minimizing the risk of rootstock rot, mold, or premature germination during storage, and guaranteeing the physiological activity and preservation quality of the rootstock.

[0036] In some of the solutions described above in this application, a filling medium is proposed to physically isolate the seed roots and maintain humidity in the basket-packing step S2. However, in practice, the base medium may have uneven moisture retention or insufficient air permeability, and lacks effective anti-mold and structural optimization functions, making the seed roots susceptible to rotting or decreased vitality due to humidity fluctuations, mold growth, or medium compaction. Therefore, this application further optimizes the composition of the filling medium to overcome the above problems.

[0037] Specifically, in the basket filling step S2, the filling medium includes a base medium and functional additives; the base medium is fine river sand or sieved fine garden soil; the functional additives are selected from at least one of vermiculite, perlite, wood ash, activated carbon powder, and decomposed and dried organic fertilizer granules.

[0038] The filling medium is designed as a combination of a base medium and functional additives, aiming to achieve more comprehensive and optimized medium performance through the synergistic effect of different components. The base medium is the main component of the filling medium, providing physical support, isolating the roots, and maintaining a basic humidity environment. The base medium can be fine river sand, with small and uniform particle diameters, good aeration and moderate drainage, and is not prone to compaction, providing a stable physical environment for the roots; or sieved fine garden soil, which, after screening to remove larger particles and impurities, has strong water and fertilizer retention capacity and contains a certain amount of organic matter, providing a microenvironment closer to natural root growth. Functional additives are used to enhance specific properties of the filling medium and address potential limitations of the base medium. For example, vermiculite, a silicate mineral that expands at high temperatures to form a porous structure, can be added, possessing good water retention, fertilizer retention, and aeration properties, improving medium ventilation and stabilizing humidity; or perlite, a lightweight and porous volcanic glassy rock that expands at high temperatures, has excellent aeration and drainage, helping to prevent medium compaction and root hypoxia. In addition, wood ash can be added. Rich in potassium and other minerals and alkaline, it has antibacterial properties and can be used to adjust the pH of the medium and inhibit mold growth. Alternatively, activated carbon powder can be added. Its porous structure has a strong adsorption capacity, capable of adsorbing harmful gases, odors, and some microbial metabolites in the medium, thereby purifying the environment and inhibiting pathogens. Furthermore, well-rotted and dried organic fertilizer granules can also be added. These granules, after thorough decomposition and drying, improve the granular structure of the medium, increase porosity, provide trace nutrients, and help maintain the loose state of the medium, preventing compaction.

[0039] By optimizing the filling medium into a composite structure of base medium and functional additives, the overall performance of the medium can be significantly improved. The base medium, such as fine river sand or sieved garden soil, provides stable physical support and basic moisture regulation, ensuring proper protection of the seed roots during packing. Building upon this, the introduction of functional additives, such as vermiculite and perlite, effectively improves the medium's pore structure and permeability, preventing compaction and ensuring smooth respiration of the seed roots. Wood ash and activated carbon powder exert their antibacterial and adsorption effects, effectively inhibiting the growth of mold and pathogens, purifying the medium environment, and thus preventing seed root rot due to disease. Well-rotted and dried organic fertilizer granules further optimize the structural stability of the medium, helping to maintain its loose state and uniform moisture content. This composite filling medium design allows the seed roots to obtain a more stable and suitable microenvironment during storage, effectively solving the shortcomings of traditional base media in terms of moisture uniformity, air permeability, mold prevention and structural stability. This minimizes the risk of seed root rot, mold growth or decreased vitality, and significantly improves the preservation quality and survival rate of Peucedanum praeruptorum seed roots.

[0040] In some of the solutions mentioned above in this application, a filling medium is proposed to isolate the seed roots and regulate the humidity and air permeability of the storage environment during the preservation process. However, in the implementation process, the composition of the filling medium lacks precise proportional control, which may lead to unstable medium performance. For example, if there is too much base medium, the water retention is too high and the air permeability is insufficient. If there is too little functional additive, the antibacterial or drainage effect is weakened, thereby increasing the risk of seed root mold, rot or abnormal germination and affecting the preservation effect.

[0041] In this regard, this application further proposes that the filling medium, by volume percentage, includes: 70%-80% base medium, 10%-15% vermiculite or perlite, and 5%-10% wood ash or activated carbon powder.

[0042] The base medium, comprising 70%-80% by volume, serves to provide primary physical support and isolation for the roots, and as the main component of the medium, provides adequate water retention. The base medium can be fine river sand or sieved fine garden soil. For example, fine river sand has good aeration and drainage, effectively preventing water accumulation; sieved fine garden soil has a certain water and fertilizer retention capacity and a relatively loose texture, which is beneficial for root respiration. Maintaining the base medium proportion within the range of 70%-80% ensures the overall structural stability and physical property balance of the medium, avoiding either excessive density leading to poor aeration due to too much base medium, or insufficient support due to too little.

[0043] The vermiculite or perlite, comprising 10%-15% by volume, primarily functions to improve the aeration and drainage of the growing medium, while also providing some water retention. For example, vermiculite is a layered silicate mineral that, after high-temperature expansion, forms a porous structure with excellent water absorption and retention, and can slowly release moisture. Its lightweight and porous nature significantly increases the aeration space of the medium. Perlite, on the other hand, is a volcanic glass that, after high-temperature expansion, forms lightweight, porous particles with excellent aeration and drainage, effectively preventing medium compaction and water retention. Maintaining the proportion of vermiculite or perlite at 10%-15% significantly increases the porosity of the medium without significantly increasing its weight, ensuring air circulation around the roots and reducing the risk of anaerobic conditions.

[0044] The wood ash or activated carbon powder, by volume percentage, is 5%-10%, serving to provide antibacterial, adsorption, and environmental regulation functions. For example, wood ash is rich in potassium and has a certain alkalinity, which can inhibit the growth of some fungi and bacteria, while also providing trace elements beneficial to the physiological activity of roots. Activated carbon powder, on the other hand, has a well-developed porous structure and strong adsorption capacity, effectively adsorbing harmful gases, odors, and toxins produced by microbial metabolism in the medium, maintaining a clean environment. Controlling the proportion of wood ash or activated carbon powder at 5%-10% ensures that its antibacterial and adsorption effects are achieved while avoiding altering the overall physicochemical properties of the medium due to excessive addition, thus ensuring the stability of the medium environment.

[0045] The above technical solution precisely defines the volume percentages of the base medium, vermiculite or perlite, and wood ash or activated carbon powder in the filling medium, thereby optimizing the overall performance of the filling medium. 70%-80% base medium provides stable physical support and moderate humidity buffering, preventing the medium from being too dense or too loose; 10%-15% vermiculite or perlite significantly enhances the medium's air permeability and drainage, effectively preventing moisture retention and mold growth; while 5%-10% wood ash or activated carbon powder introduces appropriate antibacterial and adsorption properties, inhibiting microbial growth without affecting the overall structure of the medium. This synergistic setting of these proportions ensures a balance in the filling medium's water retention, air permeability, physical isolation, and functional additions, effectively solving the performance instability problem caused by inaccurate medium composition ratios. It significantly reduces the risk of mold, rot, or premature germination of the roots during storage, thus maximizing the physiological activity and preservation effect of the Peucedanum praeruptorum roots.

[0046] In some of the embodiments described above in this application, a basket-filling step is proposed to physically isolate the seed roots and control humidity. However, in the process of implementation, due to the lack of specific container selection and filling methods, insufficient ventilation, too small spacing between seed roots, and uneven thickness of filling medium may occur, thereby increasing the risk of rot and mold.

[0047] In this regard, this application further proposes that in the basket filling step S2, the breathable container is a plastic turnover basket with breathable holes on the side wall and bottom; when filling the basket, first lay a layer of filling medium with a thickness of 3-5 cm at the bottom of the basket, then lay a layer of seed roots with a spacing of ≥1 cm, then cover it with a layer of filling medium with a thickness of 2-3 cm, repeat the above operation up to three layers, and finally cover the top layer with a layer of filling medium with a thickness of 4-7 cm.

[0048] Specifically, the breathable container is a carrier used to hold the roots of *Angelica dahurica* and the filling medium, and its design is crucial for the preservation of the roots. In this application, the breathable container is specifically a plastic turnover basket with ventilation holes on the side walls and bottom. Plastic turnover baskets are widely used in logistics and warehousing due to their lightweight, durability, ease of cleaning, low cost, and reusability. The ventilation holes on the side walls and bottom ensure that air forms multi-directional circulation paths inside the container, effectively preventing moisture from stagnating in local areas, reducing the risk of local hypoxia, and thus promoting uniform drying and gas exchange around the roots. In addition to plastic, the breathable container can also be made of other materials with good breathability and strength, such as wooden turnover boxes or metal mesh baskets with ventilation holes, as long as ventilation and load-bearing requirements are met.

[0049] When filling the baskets, first lay a 3-5 cm thick layer of packing medium at the bottom. This initial packing medium not only provides a soft cushioning layer for the roots, preventing damage from direct contact between the roots and the bottom of the container, but more importantly, it absorbs and regulates excess moisture that may accumulate at the bottom, preventing waterlogging and establishing a stable humidity base for the roots placed subsequently. This thickness range is optimized to provide sufficient cushioning and moisture absorption without taking up too much space or affecting overall air permeability.

[0050] Next, lay a layer of rootlets flat, ensuring a spacing of at least 1 cm between them. This flat laying ensures even pressure on the rootlets, preventing them from squeezing each other. Maintaining a 1 cm gap between rootlets is crucial to ensuring each rootlet is in full contact with the filling medium and receives good ventilation. This spacing design effectively reduces direct contact between rootlets, thus blocking the rapid spread of pathogens and lowering the risk of rot and mold.

[0051] Next, cover with a 2-3 cm thick layer of filler medium. This filler medium physically isolates the upper and lower layers of roots, further preventing cross-infection of pathogens. Simultaneously, it also participates in the humidity and temperature regulation inside the container, helping to maintain a stable environment around the roots through its hygroscopic and heat-insulating properties. This thickness provides effective isolation and regulation without excessively increasing the packing density, ensuring overall air permeability.

[0052] The process of laying the seed roots and covering them with the filling medium described above can be repeated, but no more than three times. Limiting the number of layers is to avoid excessive stacking inside the container. Excessive stacking can cause the lower layers of seed roots to bear excessive pressure, and obstruct internal airflow, potentially leading to localized heat accumulation and humidity imbalance, thus accelerating seed root rot. Keeping the number of layers to three or less helps ensure that each layer of seed roots within the container receives relatively uniform ventilation and humidity conditions.

[0053] Finally, a 4-7 cm thick layer of filling medium is placed on top. This thicker layer acts as a protective layer, providing additional cushioning and insulation to effectively resist temperature fluctuations and mechanical damage from the external environment. Simultaneously, it also serves as a humidity buffer at the top of the entire container structure, further stabilizing the microenvironment inside the container, ensuring consistent humidity control, and providing stable preservation conditions for the seed roots for a longer period.

[0054] Through the above technical solutions, this application significantly enhances the physical isolation and ventilation management of Angelica dahurica roots during preservation by specifying the type of breathable container and refining the details of the crating operation. The design of the plastic turnover crate with ventilation holes on the side walls and bottom ensures free airflow from multiple directions, effectively preventing moisture retention and localized oxygen deficiency inside the container, thereby promoting uniform drying of the environment around the roots. During crating, laying an initial thickness of filling medium at the bottom of the crate provides a stable humidity base for the roots and prevents water accumulation at the bottom; laying the roots flat with sufficient spacing effectively reduces direct contact between roots, blocking the transmission path of pathogens; layering the filling medium not only achieves physical isolation but also finely regulates the humidity and temperature distribution inside the container while maintaining good air permeability. Limiting the number of root layers effectively avoids internal heat accumulation and humidity imbalance that may result from excessive stacking. Finally, covering the top layer with a thicker layer of filling medium provides additional protection and humidity buffering for the entire preservation system. These measures work together to solve problems such as insufficient ventilation, excessively small spacing between seeds and roots, and uneven thickness of filling medium caused by the lack of specific container selection and filling methods in existing technologies. This significantly reduces the risk of rotting and mold growth of Peucedanum praeruptorum seeds and roots during storage, maximizes the preservation of the physiological activity of the seeds and roots, and extends the storage time.

[0055] In some of the solutions described above in this application, the temperature and humidity of the storage environment are controlled to preserve the seed roots. However, within a wide range of temperature and humidity, the seed roots may rot, mold, or sprout prematurely due to environmental fluctuations, resulting in a decrease in seed root vigor.

[0056] In this regard, this application further proposes that in step S3, the temperature of the storage environment be controlled at 2℃-10℃ and the relative humidity of the air be controlled at 35%-45%.

[0057] Specifically, controlling the storage environment temperature to 2℃-10℃ means precisely limiting the storage temperature of the Peucedanum praeruptorum roots to between 2℃ and 10℃. This temperature range aims to provide a stable and suitable dormancy condition for the roots, avoiding frost damage caused by excessively low temperatures and premature germination or excessive physiological activity caused by excessively high temperatures. This temperature control can be achieved in various ways. For example, a high-precision temperature sensor can be installed in the storage room to monitor the ambient temperature in real time and link it to an intelligent temperature control system to automatically adjust the operation of refrigeration or heating equipment (such as air conditioners or electric heaters) to maintain the temperature within the set range. Alternatively, storage facilities with good insulation properties, such as basements or semi-basement warehouses, can be used, combined with artificial ventilation and insulation measures, to maintain the temperature through regular checks and manual adjustments.

[0058] Simultaneously, controlling the relative humidity of the storage environment to 35%-45% means precisely limiting the relative humidity of the air in the storage environment for the roots of *Peucedanum praeruptorum* to between 35% and 45%. This humidity range aims to balance the roots' water requirements with the requirements for inhibiting pathogen growth, preventing the roots from wilting due to excessive dryness or rotting due to excessive humidity and the growth of mold and bacteria. This humidity control can be achieved in various ways. For example, a humidity sensor can be installed in the storage room to monitor the relative humidity in real time and automatically adjust it in conjunction with a humidifier or dehumidifier. Alternatively, the humidity can be manually adjusted by placing appropriate amounts of moisture-absorbing materials (such as silica gel or quicklime) or evaporative cooling trays in the storage environment, combined with ventilation control.

[0059] By employing the aforementioned technical solutions, the temperature and relative humidity of the storage environment are controlled within a narrower and more optimized range, providing a more stable and ideal preservation condition for the roots of *Angelica dahurica*. Precise temperature control ensures the roots remain in a deep dormant state, effectively preventing physiological disturbances caused by temperature fluctuations and significantly reducing the risk of premature germination. Simultaneously, optimized humidity control prevents the roots from losing vitality due to dehydration and effectively inhibits the growth of mold and pathogens, thereby greatly reducing the probability of rotting and mold during storage. This refined environmental control allows the roots to maintain their physiological activity and health to the maximum extent during a storage period of over 60 days, providing a high-quality seed source for planting the following year.

[0060] In some of the embodiments described above in this application, the temperature and humidity of the storage environment are controlled to preserve the seed roots. However, in the process of implementation, the temperature and humidity control may not be precise and stable enough, and cannot be monitored and adjusted in real time, resulting in fluctuations in environmental conditions and increasing the risk of rotting, mold, or premature germination.

[0061] In this regard, this application further proposes that, in step S3, the storage environment is equipped with a temperature and humidity control device, which monitors environmental parameters through a temperature and humidity sensor and feeds back to control the temperature and humidity control device to maintain the environmental temperature and humidity within a set range.

[0062] Specifically, the temperature and humidity control equipment in the storage environment refers to a device capable of actively regulating the temperature and humidity of the storage environment. For example, this equipment can be an integrated environmental control system, including a refrigeration / heating unit, a humidification / dehumidification unit, and an air circulation fan—a complete air conditioning unit; or it can be a combination of independent devices, such as an industrial air conditioner responsible for temperature regulation, while simultaneously using an independent dehumidifier or humidifier to precisely control humidity. These devices work together to provide stable storage conditions for the roots of *Peucedanum praeruptorum*. The temperature and humidity sensor is a device used to detect the air temperature and relative humidity in the storage environment in real time. It can be a digital temperature and humidity sensor, such as a DHT series sensor or a SHT series sensor, providing accurate digital signal output for easy data acquisition and processing; or it can be an analog temperature and humidity sensor, such as a thermistor or a capacitive humidity sensor, which converts analog signals into digital signals via an analog-to-digital converter. The feedback control refers to a control strategy that compares the real-time environmental parameters monitored by the temperature and humidity sensor with a preset temperature and humidity range, and automatically adjusts the operating state of the temperature and humidity control equipment based on the comparison result, so that the environmental parameters approach or remain within the set range. For example, a proportional-integral-derivative (PID) controller can be used to precisely adjust the output power or operating mode of the temperature and humidity control device based on the proportional, integral, and derivative terms of the error signal; alternatively, a simple threshold-based switching control can be used to automatically start or stop the corresponding control device when the temperature or humidity exceeds the set range. Through the above feedback control, the ambient temperature and humidity can be effectively maintained within the set range, such as the temperature of 1°C to 13°C and relative humidity of 30%-50% as defined in claim 1, or the more precise temperature of 2°C-10°C and relative humidity of 35%-45% in the above embodiments.

[0063] Through the above technical solution, when storing Angelica dahurica roots, precise, dynamic, and automatic adjustment of the storage environment's temperature and humidity can be achieved by equipping the storage environment with temperature and humidity control devices and using temperature and humidity sensors for real-time monitoring, combined with a feedback control mechanism. This closed-loop control system effectively avoids the problems of inaccurate and unstable temperature and humidity control in traditional preservation methods, significantly reducing the adverse effects of environmental fluctuations on the physiological activity of the roots. Specifically, when the ambient temperature and humidity deviate from the set range, the sensors can promptly detect the changes and, through the feedback control system, instruct the control devices to make corresponding adjustments, thereby precisely maintaining the temperature and humidity within the suitable range for root preservation. This not only maximizes the inhibition of root rot, mold, and premature germination, extending the storage time, but also ensures that the roots maintain high physiological activity and a healthy state throughout the storage period, providing a high-quality seed source for subsequent planting.

[0064] In some of the embodiments described above in this application, temperature and humidity control devices are proposed to maintain the temperature and humidity of the storage environment within a set range. However, during the implementation process, the CO2 concentration in the environment may increase due to insufficient ventilation, which may hinder the respiration of the seed roots and increase the risk of rot and mold.

[0065] In this regard, this application further proposes that the above-mentioned temperature and humidity control device includes one or more of an air conditioner, a dehumidifier, a humidifier, and a ventilation fan; the method further includes: when the CO2 concentration in the environment exceeds a set threshold, automatically starting the ventilation fan for ventilation.

[0066] Specifically, the temperature and humidity control equipment can include various components to achieve precise control of the storage environment. Among them, the air conditioner is mainly used to regulate the temperature of the storage environment, providing cooling or heating functions to maintain the ambient temperature within a set range of 1°C to 13°C. This can be achieved through a variable frequency air conditioner, which adjusts the compressor speed to achieve precise temperature control; or a constant temperature and humidity air conditioner, which can simultaneously and finely control both temperature and humidity. The dehumidifier is used to reduce the relative humidity of the air in the storage environment to prevent excessive humidity from causing mold growth in the roots of *Angelica dahurica*. This can be achieved through a refrigerated dehumidifier, which cools the air to condense water vapor to achieve dehumidification; or a rotary dehumidifier, which uses hygroscopic materials to absorb moisture from the air. The humidifier is used to increase the relative humidity of the air in the storage environment to prevent excessively low humidity from causing the roots of *Angelica dahurica* to dry out. This can be achieved through an ultrasonic humidifier, which generates water mist through ultrasonic vibration; or a wet film humidifier, which uses a water pump to deliver water to a wet film, and then uses airflow over the film to remove moisture. Ventilation fans in temperature and humidity control equipment are mainly used to promote air circulation within the storage environment, ensure the uniformity of environmental parameters, and perform ventilation under specific conditions. They can be implemented using axial fans, suitable for applications with high air volume and low pressure; or centrifugal fans, suitable for applications requiring higher pressure and longer air delivery distances.

[0067] To effectively manage the gas composition in the storage environment, this application also includes automatically activating the ventilation fan for ventilation when the CO2 concentration in the environment exceeds a set threshold. To monitor the CO2 concentration in the storage environment in real time, a CO2 sensor is required. This sensor can continuously monitor the CO2 content in the air and transmit the data to the control system. The CO2 sensor can be implemented as a non-dispersive infrared (NDIR) CO2 sensor, which determines the concentration by measuring the absorption of CO2 by infrared light of a specific wavelength; or as an electrochemical CO2 sensor, which detects CO2 through an electrochemical reaction. The set threshold is the critical CO2 concentration value that triggers the ventilation fan to start for ventilation. This threshold is usually set according to the physiological characteristics of the roots and optimal storage conditions of *Peucedanum praeruptorum* seeds, for example, it can be set to 1000 ppm or 1500 ppm. When the CO2 concentration exceeds this threshold, it indicates that the CO2 accumulation in the environment has reached a level that may adversely affect the roots. When the concentration detected by the CO2 sensor exceeds the preset threshold, the control system receives a signal and automatically commands the ventilation fan to start. When the ventilation fan is turned on, it will expel the CO2-rich air from the storage environment and introduce fresh air, thereby effectively reducing the CO2 concentration. This process can be achieved by a programmable logic controller (PLC) or microcontroller, which controls the power supply of the ventilation fan to a relay based on the input signal from the CO2 sensor.

[0068] Through the aforementioned technical solutions, the temperature and humidity control equipment not only precisely controls the temperature and humidity of the storage environment through air conditioning, dehumidifiers, and humidifiers, but also effectively solves the problem of CO2 accumulation caused by respiration in the roots of *Angelica dahurica* in a sealed storage environment by introducing ventilation fans and combining them with CO2 concentration monitoring and automatic ventilation mechanisms. When the CO2 concentration exceeds a set threshold, the automatic activation of the ventilation fans can promptly expel high-concentration CO2 and introduce fresh air, thereby avoiding the risk of respiration obstruction, decreased physiological activity, rot, and mold growth caused by excessively high CO2 concentrations in the roots. This comprehensive environmental control strategy, while maintaining suitable temperature and humidity, further optimizes the gaseous environment, maximizing the protection of the roots' health and physiological vitality during a storage period of more than 60 days, significantly improving preservation effectiveness and root survival rate.

[0069] In some of the above-mentioned solutions in this application, preservation methods are proposed to maintain the viability of seed roots by controlling the ambient temperature and humidity. However, in this process, the humidity of the filling medium may gradually decrease due to environmental evaporation or ventilation factors, resulting in insufficient water supply to the seed roots, causing drying, reduced viability, or increased risk of mold growth, thereby affecting the preservation effect.

[0070] In this regard, this application further proposes that, in order to more accurately maintain the physiological activity of the roots in the above-mentioned method for preserving Angelica dahurica seeds, the humidity of the filling medium inside the container is checked periodically during the preservation period in step S3. This check aims to continuously monitor the humidity of the microenvironment in which the roots are located, ensuring that the filling medium can provide a stable water supply to the roots. Specifically, the check can be implemented in various ways. For example, manual sampling checks can be performed periodically, by visually observing the color and state of the filling medium, or by touching it to feel its moisture level, or even by using a simple soil moisture meter. Another approach is to pre-arrange multiple humidity sensors inside the container or in the filling medium. These sensors can periodically collect humidity data of the filling medium and transmit the data to a central control system for real-time or periodic analysis, thereby achieving automated humidity monitoring.

[0071] When the humidity of the medium falls below a preset lower limit, a humidification operation is triggered. This "lower limit" is a crucial parameter whose setting directly affects the preservation effect of the seed roots. It can be determined based on previous experimental studies, the physiological characteristics of the seed roots, and the water retention capacity of different filling media, for example, by setting it to a specific percentage of the water content of the filling media. Furthermore, this lower limit can also be dynamically adjusted. For instance, it can be optimized in real time using intelligent algorithms based on a combination of factors such as the actual temperature and relative humidity of the storage environment and the physiological metabolic activities of the seed roots, to adapt to different preservation conditions.

[0072] At this point, atomization is used for humidification. The advantage of this method is that it can evenly and slowly disperse moisture into the filling medium, avoiding problems such as localized over-wetting, medium compaction, or uneven water penetration that can occur with traditional watering. Specifically, a handheld sprayer can be used for manual operation, periodically spraying the surface of the filling medium in the container evenly. Furthermore, an automatic atomization humidification system can be deployed. This system includes a water pump, nozzles, and a control unit. When the humidity sensor detects that the humidity is below the lower limit, the control unit automatically starts the water pump, spraying water mist evenly onto the filling medium through the nozzles until the humidity returns to a suitable range.

[0073] The above technical solution involves regularly checking the humidity of the filling medium within the container during storage. Based on the check results, when the medium humidity falls below a preset lower limit, precise humidification is achieved using atomization, effectively solving the problem of insufficient water supply to the seed roots due to decreased filling medium humidity. This refined humidity management ensures that the seed roots remain in a suitable humid environment throughout the entire storage period, preventing water loss and decreased vitality caused by medium dryness. It also avoids problems such as localized over-hydration, anaerobic environments, and mold growth caused by excessive or uneven humidification. The atomized humidification method allows water to penetrate the filling medium evenly and gently, maintaining the stability and balance of the microenvironment around the seed roots. This maximizes the physiological activity and health of the Peucedanum praeruptorum seed roots, significantly improving the success rate of long-term storage and the survival rate of the planted seed roots.

[0074] In some of the embodiments described above in this application, a preservation method is proposed to precisely control the storage environment and maintain the physiological activity of the seed roots. However, when the seed roots are taken out and used directly for planting after the storage period expires, the seed roots are in a dormant state due to long-term low-temperature storage, resulting in low germination rate or poor growth.

[0075] In this regard, this application further proposes that, before the seed roots are removed for planting after the storage period expires as described in step S3, a root awakening step is also included. This root awakening step aims to gradually break the physiological dormancy state of the *Peucedanum praeruptorum* seed roots caused by long-term low-temperature storage by simulating a suitable growth environment, allowing them to restore normal metabolic activity and preparing them physiologically for subsequent planting. This step is crucial to ensuring that the seed roots can germinate rapidly and grow healthily after planting. For example, root awakening can be induced by gradually increasing the ambient temperature and humidity, or by using specific light cycle treatments.

[0076] Specifically, the root awakening step includes placing the rootstock, along with its container or after removal, at 10℃-15℃ under diffused light for 3-10 days. When implementing the root awakening step, depending on operational convenience and the need for rootstock protection, one can choose to perform the awakening treatment along with its storage container, or remove the rootstock from the container first. Treating the rootstock along with its container minimizes physical damage to the rootstock and maintains the humidity stability of the filling medium. Removing the rootstock for awakening may facilitate inspection or treatment in a new medium, such as placing it in a shallow dish lined with moist sand.

[0077] Environmental conditions for the root awakening step are crucial for successfully breaking dormancy in the roots. Temperature control within the range of 10℃-15℃ aims to simulate the warm climate of spring, avoiding excessively high or low temperatures that could stress the roots and promote the recovery of enzyme activity and the initiation of cell metabolism. This temperature range can be achieved, for example, by using a temperature and humidity chamber, greenhouse, or indoor space with temperature regulation. Simultaneously, placement under diffused light provides moderate light stimulation, activating photosynthetic processes in the roots and accumulating energy for subsequent germination and growth. Diffuse light can be achieved by using a shade net, frosted glass windows, or placing the roots in a bright but indirect indoor location. The root awakening step lasts 3-10 days, a time range determined based on the physiological characteristics of the roots and experience in breaking dormancy. During this period, the roots can gradually adapt to environmental changes and complete the physiological awakening process, avoiding incomplete dormancy breaking due to a short time or excessive germination or rotting due to a long time. The specific number of days for placement can be fine-tuned based on the actual condition of the rootstock and environmental conditions. For example, the optimal root awakening effect can be determined by observing the degree of bud swelling or the germination of fine roots.

[0078] The above-described root-awakening steps effectively address the problem of low germination rates or poor growth after planting of *Angelica dahurica* seeds due to dormancy caused by long-term low-temperature storage. Before planting, the seeds are placed at a suitable temperature of 10℃-15℃ under diffused light for 3-10 days, simulating the environment before natural germination and gradually awakening their physiological activity. This gentle transition avoids the physiological shock that direct exposure to the planting environment might cause, promotes the recovery of internal metabolism and energy accumulation, and thus significantly improves the germination rate and post-planting growth vigor, laying a solid foundation for successful *Angelica dahurica* cultivation.

[0079] The following example will provide a more detailed explanation of the above technical solution: User A plans to preserve a batch of healthy Peucedanum praeruptorum (Qianhu) root seeds for planting the following spring. Traditional methods of preserving Peucedanum praeruptorum root seeds, such as direct sand storage or cellar stacking, often result in root rot, mold, or premature germination due to improper control of humidity and temperature, thus reducing root viability. This example aims to demonstrate a preservation method that allows for precise control of the storage environment, is easy to operate, and maximizes the preservation of root physiological activity.

[0080] The first step is pretreatment. User A carefully selects healthy, disease-free, and mechanically undamaged rootlets from the harvested *Peucedanum praeruptorum*. To effectively inhibit potential pathogens on the rootlet surface, User A uses a misting device to evenly spray an 800-fold dilution of 50% carbendazim wettable powder onto the rootlet surface for chemical disinfection. After chemical disinfection, User A also places the rootlets under ultraviolet light for 30 minutes for physical disinfection, further killing potential pathogens. This pretreatment step effectively inhibits the growth of pathogens on the rootlet surface, reduces the risk of rotting during storage, and avoids the rootlet rot problem caused by residual pathogens in traditional methods. After disinfection, the rootlets are left to stand in a ventilated place until their surface is completely dry to avoid introducing excess moisture during subsequent packing, which could lead to mold.

[0081] After the rootstocks were dried, User A packed them into baskets. User A selected plastic crates with ventilation holes on the sides and bottom as breathable containers. This design ensured good ventilation for the rootstocks, avoiding the localized heating and rotting caused by poor ventilation in traditional cellar storage. The preparation of the filling medium was crucial. User A mixed 75% sieved fine garden soil as the base medium, 15% vermiculite to increase water retention and aeration, and 10% wood ash to provide trace elements and inhibit pathogen growth. The filling medium was adjusted to approximately 18% humidity before use, providing a suitable microenvironment for the rootstocks during storage, solving the problem of inaccurate humidity control in traditional sand storage, which easily leads to dehydration or mold growth.

[0082] When packing the seed roots, User A first laid a 4 cm thick layer of filling medium at the bottom of the crate. Next, a layer of pre-treated seed roots was laid flat, ensuring at least 1 cm spacing between roots to prevent the spread of disease through contact. Then, a 2.5 cm thick layer of filling medium was added. User A repeated this process, placing a total of two layers of seed roots, each layer physically isolated by the filling medium, effectively avoiding the localized heating and rotting caused by excessively thick stacking as in traditional methods. Finally, a 5 cm thick layer of filling medium was placed on top, providing additional protection and moisture buffering for the seed roots. This layered packing method, combined with breathable containers and precisely proportioned filling medium, effectively prevents the seed roots from heating and rotting due to excessive stacking, and inhibits mold growth, solving the rotting and mold problems that easily occur in traditional stacking methods.

[0083] The trays containing the seed roots were placed in a specialized storage environment. This environment was designed to be dark and well-ventilated, and equipped with temperature and humidity control devices, including an air conditioner, dehumidifier, humidifier, and ventilation fan. Temperature and humidity sensors were installed inside the environment to monitor relative humidity and temperature in real time. Through a feedback control system, when the relative humidity deviates from the set range of 35%-45% or the temperature deviates from the set range of 2℃-10℃, the corresponding control devices automatically activate to adjust, ensuring a precise and stable storage environment and avoiding the problem of mold growth caused by imprecise humidity and ventilation control in traditional methods.

[0084] In addition, the storage environment is equipped with a CO2 concentration sensor. When the CO2 concentration in the environment exceeds a preset threshold, the ventilation fan will automatically start to exchange air, so as to avoid CO2 accumulation from adversely affecting the physiological activities of the seed roots and further protect the vitality of the seed roots.

[0085] During the 90-day storage period, User A regularly checks the humidity of the medium filling the container. For example, every two weeks, User A randomly selects a container and checks the humidity of the medium. When the medium humidity is found to be below the lower limit of 15%, User A uses a misting method to replenish the medium appropriately, ensuring that the roots are always in a suitable microenvironment. This effectively prevents the roots from losing water or losing vitality due to the drying of the medium, solving the problem of maintaining medium humidity in traditional methods.

[0086] Before removing the rootstocks for planting the following spring, User A will perform a root awakening step after the storage period expires. User A will place the container of rootstocks in an environment with diffused light at 12°C for 7 days. This step helps the rootstocks gradually adapt to the external environment, restore their physiological activity, and prepare them for subsequent planting. This maximizes the preservation of the rootstocks' physiological activity and avoids the situation where rootstocks become unusable due to decreased vitality, as is often the case with traditional methods.

[0087] To scientifically verify the effectiveness of the technical solution in this embodiment, the applicant designed the following three sets of comparative experiments. All experiments were conducted within the same time period and from the same batch of Peucedanum praeruptorum roots to control variables to the greatest extent possible.

[0088] 1. Experimental grouping and treatment methods: 2. Experimental period and observation points Storage period: 62 days (from December 5 of the current year to February 5 of the following year).

[0089] Key observation points: Mid-storage (day 30): 30 root segments were randomly selected from each replicate (90 segments in total per group) for non-destructive inspection (appearance), and early mold growth or germination was recorded.

[0090] At the end of storage (day 62): all seed roots were inspected and counted one by one.

[0091] Planting verification period: After storage, 100 segments (300 segments in total per group) were randomly selected from each replicate of intact seed roots for standardized field planting, and agronomic traits were observed in the later stage.

[0092] 3. Measurement Indicators and Data Statistics Key indicators: Rot rate: The proportion of root tissue that is soft, water-soaked, or ulcerated.

[0093] Mold rate: The proportion of visible mold hyphae or spores on the root surface.

[0094] Early germination rate: The proportion of buds with a length exceeding 2 mm during storage.

[0095] Overall integrity rate: 100% - (rot rate + mold rate + premature germination rate).

[0096] Data presentation: All rates are expressed as mean ± standard deviation, and the data are from three independent replicates. Analysis of variance (ANOVA) was performed using statistical software, and p < 0.05 was used as the criterion for statistical significance.

[0097] Seed root condition after storage (day 62): Conclusion: The integrity rate of the method of the present invention is significantly higher than that of the traditional method (P<0.01).

[0098] Key growth indicators after planting (60 days post-planting): Conclusion: The seed roots preserved by the method of this invention have significant advantages in field production performance.

[0099] In summary, the overall integrity rate of the method group of the present invention (96.5 ± 0.5%) was significantly higher than that of control group 1 (73.8 ± 1.8%) and control group 2 (28.5 ± 2.5%), and the key growth of the planting was also better than that of control group 1 and control group 2.

[0100] The above description describes specific embodiments of the present invention, but the scope of protection of the present invention is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in the present invention, and these modifications or substitutions should all be covered within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A method for preserving the rhizome of Peucedanum graveolens, characterized in that, The method comprises the following steps: S1. Pretreatment: healthy peucedani radix is selected, surface sterilized, and then left to dry; S2. Basketing: the dried peucedani radix is alternately layered with filling medium with a humidity of 15%-22% into a breathable container, wherein the peucedani radix is placed in three layers at most, and the peucedani radix in adjacent layers is physically isolated by the filling medium; S3. Storage: the container with the peucedani radix is placed in a light-proof and ventilated storage environment, the relative humidity of the air in the storage environment is controlled to be 30%-50%, and the temperature is controlled to be 1-13℃, and the storage duration can be up to 60 days or more.

2. The method of claim 1, wherein the root of the radix peucedani is stored at a temperature of -20°C to 0°C. In step S1, the surface sterilization comprises spraying a disinfectant by using a fogging device, the disinfectant is selected from at least one of 75% ethanol, 0.1% potassium permanganate solution, 50% carbendazim wettable powder 800 times liquid, 25% azoxystrobin suspension concentrate 1500 times liquid, 1% garlicin extract, or 100 billion CFU / g bacillus subtilis wettable powder 500 times liquid; and / or, before or after spraying the disinfectant, a physical sterilization step of irradiation by using an ultraviolet lamp or fumigation by using low-concentration ozone is further included.

3. The method of claim 1, wherein the root of the radix peucedani is stored at a temperature of -20°C to 0°C. In step S2, the filling medium comprises a base medium and a functional additive; The base medium is fine river sand or sieved fine garden soil; The functional additive is selected from at least one of vermiculite, perlite, wood ash, activated carbon powder, and granular composted dry organic fertilizer.

4. The method of claim 3, wherein the root of the radix peucedani is stored at a temperature of 0 to 10°C and a relative humidity of 40 to 70% for 1 to 3 months. The filling medium comprises, in terms of volume percentage, 70%-80% base medium, 10%-15% vermiculite or perlite, and 5%-10% wood ash or activated carbon powder.

5. The method of claim 1, wherein the root of the radix peucedani is stored at a temperature of -20°C to 0°C. In step S2, the breathable container is a plastic turnover basket with breathable holes on the side wall and the bottom; when basketing, a layer of filling medium with a thickness of 3-5 cm is first laid on the basket bottom, then a layer of peucedani radix is laid flat, the distance between the peucedani radix is ≥1 cm, then a layer of filling medium with a thickness of 2-3 cm is covered, the above operation is repeated for up to three layers, and finally a layer of filling medium with a thickness of 4-7 cm is covered on the uppermost layer.

6. The method of claim 1, wherein the root of the radix peucedani is stored at a temperature of -20°C to 0°C. In step S3, the temperature of the storage environment is controlled to be 2-10℃, and the relative humidity of the air is controlled to be 35%-45%.

7. The method of claim 1, wherein the root of the radix peucedani is stored at a temperature of 0 to 10°C. In step S3, the storage environment is equipped with a temperature and humidity control device, the environmental parameters are monitored by a temperature and humidity sensor, and the temperature and humidity control device is feedback controlled to maintain the environmental temperature and humidity within the set range.

8. The method of claim 7, wherein the root of the radix peucedani is stored at a temperature of -20°C to 0°C. The temperature and humidity control device comprises one or more of an air conditioner, a dehumidifier, a humidifier, and a ventilation fan; the method further comprises: when the CO2 concentration in the environment exceeds a set threshold, the ventilation fan is automatically started for ventilation.

9. The method of claim 1 to 8, wherein the radix paeoniae rubra is stored at a temperature of -20°C to 0°C. During the storage period in step S3, the humidity of the filling medium in the container is also regularly checked, and when the medium humidity is below the lower limit, the medium is humidified by fogging.

10. The method of claim 1 to 8, wherein the radix paeoniae rubra is stored at a temperature of -20°C to 0°C. After the storage period in step S3, before the peucedani radix is taken out for planting, a root awakening step is further included: the peucedani radix is placed at 10-15℃ under scattered light for 3-10 days, together with the container or after being taken out.

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