Pesticide composition and application thereof

Abstract

The invention belongs to the technical field of pesticides, and discloses a pesticide composition and application thereof.The pesticide composition comprises an active component A and an active component B. The active component A is a compound shown in the formula I, the active component B is any one of pyflubumide, vaniliprole, cyflumetofen or flufenoxystrobin, and the mass ratio of the active component A to the active component B is 1: 40-40: 1. The pesticide composition disclosed by the invention has an obvious synergistic effect on common phytophagous mites of fruit trees, vegetables, ornamental plants, cotton and the like, and has an obvious control effect on pest mites.

Description

[0001] This invention application is a divisional application of application number 202311307669.0, filed on October 11, 2023, entitled "A pesticide composition and its application". Technical Field

[0002] This invention belongs to the field of pesticide technology and discloses a pesticide composition and its application. Background Technology

[0003] Compound I is an acaricide with excellent control effects against common plant mites. Its chemical name is (4'-chloro-6-fluoro-4-methyl-[1,1'-biphenyl-3-yl])(2,2,2-trifluoroethyl) sulfide, and its structural formula is as follows:

[0004] Pyflubumide, CAS Registry Number: 926914-55-8, is a novel formamide acaricide discovered and developed by Nippon Kogyo Co., Ltd. It is the only acaricide with a formamide structure and a novel inhibitor of mitochondrial complex II in the respiratory chain. This acaricide exhibits high control efficacy against phytophagous mites, including spider mites resistant to conventional acaricides. Its structural formula is shown below:

[0005] Vaniliprole, CAS Registry Number: 145767-97-1, chemical name 1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-5-[(4-hydroxy-3-methoxyphenyl)methyleneamino]-4-(trifluoromethylthio)pyrazole-3-carboxynitrile, its structural formula is shown below:

[0007] Cyflumetofen, CAS Registry Number: 400882-07-7, is a novel benzoylacetonitrile acaricide. Its mechanism of action involves in vivo deesterification to form a hydroxyl structure, which interferes with and inhibits mitochondrial protein complex II, hindering electron (hydrogen) transfer, disrupting phosphorylation, and ultimately paralyzing and killing the mites. Its structural formula is shown below:

[0009] Flufenoxystrobin, CAS Registry Number: 918162-02-4, is a methoxyacrylate compound with high fungicidal activity and significant acaricidal activity. It is effective against common mites. Its structural formula is shown below:

[0011] Mites, especially phytophagous mites, are a global pest that causes significant losses to agricultural and forestry production every year. Therefore, the control of agricultural mites is particularly important. However, because mites readily develop resistance, the effectiveness of currently available commercially available acaricides generally decreases quickly due to resistance. Therefore, how to use pesticides rationally and effectively to control phytophagous mites has become a major issue in agricultural production. Summary of the Invention

[0012] In view of the shortcomings of the prior art, the present invention provides a pesticide composition for controlling plant mites, which can effectively delay the development of mites' resistance to pesticides and extend the application time of the pesticide.

[0013] To achieve the above objectives, the present invention adopts the following technical solution: a pesticide composition comprising active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula I, and active ingredient B is any one of pyflubumide, vaniliprole, dicofol or flufenoxuron. Furthermore, the mass ratio of active ingredient A to active ingredient B in the pesticide composition is 1:40 to 40:1; Furthermore, the active ingredient B is pyflubumide, and the mass ratio of active ingredient A to active ingredient B is 1:30~40:1; Furthermore, the active ingredient B is pyflubumide, and the mass ratio of active ingredient A to active ingredient B is 1:20~32:1; Furthermore, the active ingredient B is vaniliprole, and the mass ratio of active ingredient A to active ingredient B is 1:36 to 20:1; Furthermore, the active ingredient B is vaniliprole, and the mass ratio of active ingredient A to active ingredient B is 1:28 to 10:1; Furthermore, the active ingredient B is fluoroacetate, and the mass ratio of active ingredient A to active ingredient B is 1:38~41:1; Furthermore, the active ingredient B is fluoroacetate, and the preferred mass ratio of the active ingredient is 1:24 to 36:1. Furthermore, the active ingredient B is fluopyram, and the mass ratio of active ingredient A to active ingredient B is 1:35~32:1; Furthermore, the active ingredient B is fluopyram, and the mass ratio of active ingredient A to active ingredient B is 1:24 to 28:1.

[0014] Furthermore, based on a total weight of 100 wt%, the total weight of active ingredient A and active ingredient B accounts for 1 wt% to 80 wt% of the total weight of the agricultural composition. Furthermore, the pesticide composition includes, in addition to the active ingredient, pesticide-permitted auxiliary ingredients; Furthermore, the auxiliary ingredients are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, preservatives, stabilizers, synergists, and carriers. Further, the wetting agent is selected from one or more of alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soapberry powder, soapberry powder, SOPA, detergents, emulsifiers 2000 series, and wetting and penetrating agents F; and / or The dispersant is selected from one or more of the following: lignin sulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or The thickener is selected from one or more of xanthan gum, polyvinyl alcohol, organobentonite, magnesium aluminum silicate, and carboxymethyl cellulose; and / or The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or The emulsifier is selected from one or more of the following: fatty alcohol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, phenethylphenol polyoxyethylene polyoxypropylene ether, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, alkylbenzene sulfonate, styrene-phenol polyoxyethylene ether, and fatty acid polyoxyethylene ester; and / or The defoamer is selected from silicone oil, C 10 ~C 20Saturated fatty acid compounds, C8-C 10 One or more of fatty alcohols or silicone compounds; and / or The preservative is selected from one or more of sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, and methyl p-hydroxybenzoate; and / or The stabilizer is selected from one or more of the following: disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, and epoxidized vegetable oil; and / or The synergist is selected from synergistic phosphorus, synergistic ether; and / or The carrier is selected from one or more of kaolin, bentonite, attapulgite, light calcium carbonate, diatomaceous earth, and precipitated silica; and / or The solvent is selected from one or more of benzene, toluene, xylene, methanol, ethanol, isopropanol, n-butanol, diesel oil, N,N-dimethylformamide, cyclohexanone, ethyl acetate, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, solvent oil, vegetable oil, vegetable oil derivatives, and deionized water; and / or All of the above auxiliary ingredients are commercially available.

[0015] Furthermore, the pesticide composition can be prepared as a solid formulation or a liquid formulation; Furthermore, the solid dosage form is selected from powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules; The liquid formulation is selected from soluble agents, colloids, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspension agents, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions. Furthermore, the solid formulation is a water-dispersible granule or a wettable powder, and the liquid formulation is selected from emulsifiable concentrates, water emulsions, microemulsions, dispersible oil suspensions, or suspensions.

[0016] The present invention also discloses the application of the pesticide composition described above for the control of phytophagous mites.

[0017] Furthermore, the phytophagous mites mentioned are any one or more of the following: two-spotted spider mite, carmine spider mite, citrus spider mite, truncated spider mite, hawthorn spider mite, citrus spider mite, or spider mite. Furthermore, the phytophagous mites mentioned are two-spotted spider mites or carmine spider mites.

[0018] The beneficial effects of this invention are: 1. The pesticide composition of the present invention has a significant synergistic effect, which can reduce the number of pesticide applications and the amount of single agent used; 2. The pesticide composition of the present invention can effectively slow down the development and progression of mite resistance, and is safe for crops and the environment. Detailed Implementation

[0019] To make the technical solution, objectives and advantages of the present invention clearer, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0020] Formulation preparation example Preparation Example 1: 18% Formula I compound pyflubumide suspension (2:1) Formula composition: 12% Formula I compound, 6% pyflubumide, 1% Gelbert alcohol polyoxyethylene ether, 2% succinate sulfonate, 2% alkylphenol polyoxyethylene ether phosphate, 2% alkylaryl polyoxyethylene ether polyoxypropylene ether, 1% sodium polycarboxylate, 0.2% xanthan gum, 1% magnesium aluminum silicate, 5% ethylene glycol, 0.2% sodium benzoate, 0.5% silicone oil, deionized water to make up the balance; Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0021] Preparation Example 2: 24% of Formula I compound pyflubumide water-dispersible granules (1:5) Formula composition: 4% Formula I compound, 20% pyflubumide, 8% lignin sulfonate, 3% sodium dodecyl sulfate, 4% naphthalene sulfonate formaldehyde condensate, 5% silica, 23% starch, and kaolin to make up the balance.

[0022] Preparation method: According to the formula ratio, add the active ingredients to the carrier, and add surfactants and other functional additives to it. Mix, and after air jet milling, add 10-25% water. Then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0023] Preparation Example 3: 24% of Formula I compound·vaniliprole suspension (1:3) Formula composition: 6% Formula I compound, 18% vaniliprole, 1% fatty alcohol polyoxyethylene ether, 1% sodium polycarboxylate, 3% fatty alcohol polyoxyethylene ether sulfate, 2% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 0.5% magnesium aluminum silicate, 0.2% xanthan gum, 5% ethylene glycol, 0.2% potassium benzoate, 0.5% silicone oil, deionized water to make up the balance.

[0024] Preparation method: Same as in preparation example 1.

[0025] Preparation Example 4: 30% of Formula I compound vaniliprole water-dispersible granules (5:1) Formula composition: 25% Formula I compound, 5% vaniliprole, 8% sodium polycarboxylate, 2% BX (a type of sizing agent), 5% naphthalene sulfonate formaldehyde condensate, 5% ammonium sulfate, and kaolin to make up the balance.

[0026] Preparation method: Same as in preparation example 2.

[0027] Preparation Example 5: 20% Formula I compound·flufenicol suspension (1:1) Formula composition: 10% Formula I compound, 10% fluoroacetate, 0.5% alkylphenol polyoxyethylene ether, 3% alkylphenol polyoxyethylene ether phosphate, 1% sodium lignosulfonate, 1% sodium polycarboxylate, 0.25% xanthan gum, 5% glycerol, 0.1% sodium benzoate, 0.5% silicone oil, deionized water to make up the balance.

[0028] Preparation method: Same as in preparation example 1.

[0029] Preparation Example 6: 16% Compound I·Fluoropropyl ester emulsifiable concentrate (15:1) Formula composition: 15% Formula I compound, 1% fluoroacetate, 15% N-methylpyrrolidone, 15% cyclohexanone, 10% alkylphenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 10% DMF, and methyl oleate to make up the balance.

[0030] Preparation method: According to the formula ratio, the measured active ingredients, solvents and co-solvents are added to the mixing tank and stirred to dissolve them. Then, the emulsifier is added, and the remaining solvent is used to make up the balance. The mixture is stirred evenly in the mixing tank and filtered to obtain the emulsifiable oil required by the present invention.

[0031] Preparation Example 7: 16% Formula I compound·flufenicol emulsifiable concentrate (3:1) Formulation composition: 12% Formula I compound, 4% fluopyram, 10% EO / PO block copolymer, 15% acetophenone, 8% propylene glycol methyl ether, 2% calcium dodecylbenzenesulfonate, xylene to make up the balance.

[0032] Preparation method: Same as in preparation example 6.

[0033] Preparation Example 8: 24% Formula I compound·flufenicol suspension (1:5) Formula composition: 4% Formula I compound, 20% fluopyram, 1% fatty alcohol polyoxyethylene ether, 2% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 2% succinate sulfonate, 4% alkylphenol polyoxyethylene ether phosphate, 1% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, deionized water to make up the balance; Preparation method: Same as in preparation example 1.

[0034] All the preparations prepared in the above examples have been tested and found to meet the quality and technical indicators required for the corresponding preparations. The preparations obtained are qualified preparations recognized in this field.

[0035] Example 1: Indoor activity test of compound pesticide composition against adult two-spotted spider mites

[0036] Test basis: The test was conducted in accordance with NY / T 1154.12-2008 "Guidelines for the determination of biological activity of pesticides in the laboratory - Insecticides - Part 12: Tetranychus slide immersion method".

[0037] Experimental target: Two-spotted spider mite ( Spider mite Koch) Female adult mite.

[0038] Test reagents: Formula I compound technical grade, pyflubumide technical grade, vaniliprole technical grade, diflubenzuron technical grade, and flufenoxuron technical grade.

[0039] Experimental Method: Select female adult mites that were kept indoors and were in the same physiological state. Cut 2cm lengths of double-sided tape and stick them to one end of a glass slide. Select healthy female adult mites and stick them onto the double-sided tape, 30 mites per slide. After treatment, place the mites in a container lined with a damp sponge, cover the container, and place it at (25±1)℃. After 2 hours, examine the mites under a microscope, remove dead, injured, and inactive individuals, and replenish the slide to 30 mites.

[0040] The technical grade compound of formula I was dissolved in N,N-dimethylformamide. The technical grades of pyflubumide, vaniliprole, diflubenzuron, and flufenoxuron were dissolved in acetone. The dissolved technical grades were then prepared as stock solutions, which were further diluted with 0.1% Tween-80 aqueous solution to create five series of mass concentration gradients. Glass slides were immersed in the solutions and gently shaken for 5 seconds. Excess solution was absorbed with absorbent paper, and the slides were placed in a white porcelain dish lined with a damp sponge. The dish was then covered with a translucent plastic film. Each treatment was repeated four times, and a blank control containing no solutions (containing all organic solvents and emulsifiers) was included.

[0041] Feeding and observation: The treated mites were fed and observed at (25±1)℃ with a photoperiod L:D=(16:8)h. After 48 hours of treatment, the mites were checked for mortality, and the total number of mites and the number of dead mites were recorded.

[0042] Data statistics and analysis: Based on the survey data, the corrected mortality rates for each treatment were calculated using the following formula, and the results were rounded to two decimal places.

[0043]

[0044] In the formula: P — Mortality rate, expressed as a percentage (%); K —This indicates the number of dead insects, expressed in heads; N — This indicates the total number of insects treated, in units of heads.

[0045]

[0046] In the formula: P 1 —Adjusted mortality rate, in percentages (%); P t —The mortality rate is expressed as a percentage (%). P 0 — Mortality rate in blank control group, expressed as a percentage (%).

[0047] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the corrected mortality rate formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0048] The LC was determined using the DPS statistical analysis system. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0049] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0050] In the formula: AT —Measured toxicity index of the mixture; S LC of standard acaricides 50 The unit is milligrams per liter (mg / L); MLC of the mixture 50 The unit is milligrams per liter (mg / L).

[0051]

[0052] In the formula: THIRTY —Theoretical toxicity index of the mixture; YOU A —A. Toxicity index of drug A; P A —The percentage content of drug A in the mixture, expressed as a percentage (%). YOU B —Toxicity index of drug B; P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0053]

[0054] In the formula: CTC —Cotoxicity coefficient; AT —Measured toxicity index of the mixture; THIRTY —Theoretical toxicity index of mixed preparations.

[0055] Co-toxicity coefficient of compound CTC ≥120 exhibits a synergistic effect; CTC≤80 It exhibits antagonistic effects; 80 < CTC <120 exhibits an additive effect.

[0056] The relevant indoor activity tests are shown in the table below:

[0057]

[0058]

[0059]

[0060] As shown in Table 1-4 of the indoor activity test results, compound I, when combined with any one of pyflubumide, vaniliprole, diflubenzuron, or flufenoxuron at an appropriate mass ratio, exhibits excellent control effect against two-spotted spider mites. Compound I exhibits a synergistic effect against Tetranychus simulans when its mass ratio with pyflubumide is 1:30–40:1, with a co-toxicity coefficient greater than 120. Compound I also exhibits a synergistic effect against Tetranychus simulans when its mass ratio with vaniliprole is 1:36–20:1, with a co-toxicity coefficient greater than 120, and the highest co-toxicity coefficient of 175.542 when the mass ratio is 1:3. Compound I also exhibits a synergistic effect when its mass ratio with dicofol is 1:38–41:1, with a co-toxicity coefficient greater than 120, and the highest co-toxicity coefficient of 211.273 when the mass ratio is 1:1. Compound I also exhibits a synergistic effect when its mass ratio with fluopyram is 1:35–32:1, with a co-toxicity coefficient greater than 120.

[0061] Field efficacy test of the compound composition against strawberry spider mites

[0062] Test reference: The test was conducted in accordance with GB / T 17980.17-2000 "Guidelines for Field Efficacy Tests of Pesticides (I) Control of Spider Mites on Legumes and Vegetables with Acaricides".

[0063] Experimental location: Fruit and vegetable greenhouses in Shanghui Village, Linhai City, Zhejiang Province. The cultivation and management of each experimental plot were consistent, and the strawberry growth was relatively uniform.

[0064] The crop tested was strawberry (Red Beauty).

[0065] Experimental targets: The main harmful species are Tetranychus carmineus and Tetranychus two-spotted.

[0066] Experimental Design: The experiment consisted of 10 treatments, each repeated 4 times. The plots were randomly arranged, with each plot measuring 15m². 2 On February 5, 2022, each treatment area was treated with pesticide diluted with water according to the experimental design. The pesticide was applied using a backpack electric sprayer to evenly spray the entire strawberry plant.

[0067] Survey and statistics: 20 plants were randomly selected from each plot, and one leaf of each plant was marked. The number of surviving spider mites on the marked leaf was investigated each time. A total of 4 surveys were conducted. The initial mite population was investigated before application of the pesticide, and 3, 7 and 14 days after application. The number of live mites was recorded, and the mite population reduction rate and control effect of each treatment area were calculated.

[0068] During the experiment, observe whether the agent causes phytotoxicity to crops and whether it has adverse effects on other target organisms.

[0069] The calculation formula is as follows:

[0070] The experimental results are shown below:

[0071] Field efficacy trials showed that the pesticide composition described in this invention has a good control effect on strawberry spider mites, and each agent has a fast-acting effect and a long-lasting effect on strawberry spider mites. No phytotoxicity was observed in the strawberry plants during the entire trial, nor were any adverse effects observed on other target organisms.

[0072] Although the present invention has been described in detail above with general description and specific embodiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A pesticide composition, characterized in that, The pesticide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula I: (Formula I), wherein the active ingredient B is any one of vaniliprole, dicofol, or flufenoxuron.

2. The pesticide composition according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B in the pesticide composition is 1:40 to 40:

1.

3. The pesticide composition according to claim 1, characterized in that, The active ingredient B is vaniliprole, and the mass ratio of active ingredient A to active ingredient B is 1:36 to 20:1; preferably, the mass ratio is 1:28 to 10:

1. The active ingredient B is dicofol, and the mass ratio of active ingredient A to active ingredient B is 1:38~41:1; preferably, the mass ratio is 1:24~36:

1. The active ingredient B is fluopyram, and the mass ratio of active ingredient A to active ingredient B is 1:35~32:1; preferably, the mass ratio is 1:24~28:

1.

4. The pesticide composition according to claim 1, characterized in that, The total weight of the pesticide composition is calculated as 100 wt%, and the total weight of active ingredient A and active ingredient B accounts for 1 wt% to 80 wt% of the total weight of the agricultural composition.

5. The pesticide composition according to claim 1, characterized in that, In addition to the active ingredient, the pesticide composition also includes pesticide-permitted auxiliary ingredients.

6. The pesticide composition according to claim 5, characterized in that, The auxiliary ingredients are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, preservatives, stabilizers, synergists, and carriers.

7. The pesticide composition according to claim 1, characterized in that, The pesticide composition can be prepared as a solid formulation or a liquid formulation.

8. The pesticide composition according to claim 7, characterized in that, The solid dosage form is selected from powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules. The liquid formulation is selected from soluble agents, colloids, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspension agents, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions. Preferably, the solid formulation is a water-dispersible granule or a wettable powder, and the liquid formulation is selected from emulsifiable concentrates, water emulsions, microemulsions, dispersible oil suspensions, or suspensions.

9. The application of the pesticide composition according to any one of claims 1-8 for the control of phytophagous mites.

10. The application according to claim 9, characterized in that, The phytophagous mites mentioned are any one or more of the following: two-spotted spider mite, carmine spider mite, citrus spider mite, truncated spider mite, hawthorn spider mite, citrus spider mite, or spider mite. Preferably, the phytophagous mites are two-spotted spider mites or carmine spider mites.

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