Fermentation method applying'twice irradiation sterilization '
By subjecting the fermentation raw materials and mature products to two irradiation sterilization processes, the problem of contamination by miscellaneous bacteria and bacteriophages during fermentation is solved, ensuring smooth fermentation and extending the product's shelf life. This method is applicable to fermentation fields such as tobacco, tea, grains, and feed.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-27
- Publication Date
- 2026-03-31
AI Technical Summary
During the fermentation process, fermentation raw materials and mature fermentation products are easily contaminated by miscellaneous bacteria and bacteriophages, leading to fermentation failure or reduced yield, which is difficult to control effectively with existing technologies.
The fermentation raw materials and mature products are sterilized by irradiation using an accelerator or isotope radiation source, which is carried out twice, before and after fermentation, to kill miscellaneous bacteria and bacteriophages, and to add beneficial microbial strains to extend the product's shelf life.
It effectively kills unwanted bacteria and bacteriophages, ensuring a smooth fermentation process, increasing yield, and extending the shelf life of the fermented product.
Smart Images
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of fermentation applications and relates to a fermentation method involving two-stage irradiation sterilization: "irradiation sterilization of fermentation raw materials before fermentation" and "irradiation sterilization of fermented mature products after fermentation maturity". Background Technology
[0002] The fermentation process requires controlling the growth of unwanted microorganisms. Conventional methods for controlling these microorganisms include rigorous equipment sterilization, air heating sterilization, and automating the fermentation process as much as possible. However, if the fermentation feedstock is contaminated with unwanted microorganisms or bacteriophages, it will affect the fermentation process, leading to reduced product yield. In severe cases, it can even cause the entire fermentation process to fail, resulting in the complete spoilage of the fermented product.
[0003] Currently, fermentation raw materials can be processed using various pre-fermentation methods, including untreated raw material fermentation, fermentation after baking, and fermentation after cooking. Once fermentation is complete, methods to stop it typically include using preservatives, adding alcohol, low-temperature storage, high-temperature treatment or sun exposure, and distillation.
[0004] The sterilization of fermentation raw materials and the cessation of fermentation of fermentation products after fermentation maturity are both problems that need to be solved in the fermentation process.
[0005] Irradiation sterilization uses electron beams or gamma rays generated by irradiation devices such as accelerators or isotope radiation sources (e.g., cobalt-60 gamma sources) to penetrate the goods to be irradiated and act on microorganisms, directly or indirectly destroying their ribonucleic acid, proteins, and enzymes, thereby killing the microorganisms and achieving the effect of disinfection and sterilization. Summary of the Invention
[0006] This invention utilizes an irradiation device, typically 1-10 MeV, or an isotope radiation source (such as a cobalt-60 gamma source), to intervene in the fermentation process. "Irradiation sterilization of fermentation raw materials before fermentation" kills contaminating bacteria and bacteriophages to achieve the required sterilization standards. "Irradiation sterilization of the mature fermented product after fermentation" stops fermentation and extends the shelf life of the mature product, maintaining its quality. It can be widely applied in the fermentation fields of tobacco, tea, grains, and feed.
[0007] The present invention adopts the following technical solution: (1) Before fermentation, the fermentation raw materials are irradiated to sterilize them, killing miscellaneous bacteria and bacteriophages to achieve the required sterilization standards. The irradiation dose is usually selected as 1-5 KGy, and the specific irradiation dose parameter is selected according to the fermentation product.
[0008] (2) After the fermentation raw materials are sterilized by irradiation, an appropriate amount of beneficial microbial strains for specific processes are added, and fermentation is carried out according to conventional fermentation processes.
[0009] (3) After fermentation is complete, the mature fermented product is sterilized by irradiation to stop fermentation and extend the shelf life of the mature fermented product. The irradiation dose is usually selected as 1-5 KGy, and the specific irradiation dose parameter is selected according to the fermented product. Attached Figure Description
[0010] Figure 1 This is a schematic diagram of the irradiation device of an accelerator, used for irradiation sterilization.
[0011] Figure 2 This is a schematic diagram of an irradiation device using an isotope radioactive source (such as a cobalt-60 gamma source) for irradiation sterilization.
[0012] Figure 3 This is a schematic diagram of the fermentation method that uses "double irradiation sterilization". Detailed Implementation
[0013] The technical solution of the present invention will be further described below with reference to the accompanying drawings and specific embodiments.
[0014] To better illustrate the present invention and facilitate understanding of its technical solutions, the present invention is presented in a typical but non-limiting manner. The following is an example: Example
[0015] This embodiment provides a fermentation method employing "double irradiation sterilization" applied to tobacco fermentation. The method includes the following steps: (1) Before fermentation, the fermentation raw material - tobacco leaves - is irradiated to sterilize and kill miscellaneous bacteria and bacteriophages to achieve the required sterilization standard. The irradiation dose is usually selected as 1-5 KGy, and the specific irradiation dose parameter is selected according to the fermentation product.
[0016] (2) After the raw material for fermentation, tobacco leaves are irradiated and sterilized, an appropriate amount of microbial strains beneficial to specific flavor processes are added, and fermentation is carried out according to conventional fermentation processes.
[0017] (3) After fermentation is complete, the mature fermented product—tobacco leaves—is sterilized by irradiation to stop fermentation and extend the shelf life of the mature product. The irradiation dose is usually selected as 1-5 KGy, and the specific irradiation dose parameter is selected according to the fermented product. Example
[0018] This embodiment provides a fermentation method employing "double irradiation sterilization" applied to tea fermentation. The method includes the following steps: (1) Before fermentation, the fermentation raw material - tea leaves - is irradiated to sterilize and kill miscellaneous bacteria and bacteriophages to achieve the required sterilization standard. The irradiation dose is usually selected as 1-5 KGy, and the specific irradiation dose parameter is selected according to the fermentation product.
[0019] (2) Fermentation raw material - After tea leaves are sterilized by irradiation, an appropriate amount of microbial strains that are beneficial to the specific flavor process are added, and fermentation is carried out according to the conventional fermentation process.
[0020] (3) After fermentation is complete, the mature fermented product - tea leaves - is sterilized by irradiation to stop fermentation and extend the shelf life of the mature fermented product. The irradiation dose is usually selected as 1-5 KGy, and the specific irradiation dose parameter is selected according to the fermented product. Example
[0021] This embodiment provides a fermentation method employing "double irradiation sterilization" for the fermentation of grain-based edible alcohol. The method includes the following steps: (1) Before fermentation, the fermentation raw material - grain - is irradiated to sterilize it, killing miscellaneous bacteria and bacteriophages to achieve the required sterilization standard. The irradiation dose is usually selected as 1-5 KGy, and the specific irradiation dose parameter is selected according to the fermentation product.
[0022] (2) After the raw materials for fermentation are sterilized by irradiation, an appropriate amount of microbial strains that are beneficial to the specific flavor process are added, and fermentation is carried out according to the conventional fermentation process.
[0023] (3) After fermentation is complete, the mature fermented product - grain - is sterilized by irradiation to stop fermentation and extend the shelf life of the mature fermented product. The irradiation dose is usually selected as 1-5 KGy, and the specific irradiation dose parameter is selected according to the fermented product. Example
[0024] This embodiment provides a fermentation method employing "double irradiation sterilization" for feed fermentation. The method includes the following steps: (1) Before fermentation, the fermentation raw materials - feed raw materials - are irradiated to sterilize them, killing miscellaneous bacteria and bacteriophages to achieve the required sterilization standards. The irradiation dose is usually selected as 1-5 KGy, and the specific irradiation dose parameter is selected according to the fermentation product.
[0025] (2) Fermentation raw materials - After the feed raw materials are irradiated and sterilized, appropriate amounts of microbial strains with specific flavoring processes are added, and fermentation is carried out according to conventional fermentation processes.
[0026] (3) After fermentation is complete, the mature fermented product - feed - is sterilized by irradiation to stop fermentation and extend the shelf life of the mature fermented product. The irradiation dose is usually selected as 1-5 KGy, and the specific irradiation dose parameter is selected according to the fermented product.
[0027] The applicant declares that the detailed features of the present invention are illustrated by the above embodiments, but the present invention is not limited to the above detailed features, that is, it does not mean that the present invention must rely on the above detailed features to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent substitutions for selected components, additions of auxiliary components, and selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.
[0028] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.
[0029] It should also be noted that the various specific technical features described in the above embodiments can be combined in any suitable manner without contradiction. To avoid unnecessary repetition, this invention provides for various possible combinations. The combinations of energy will not be explained separately.
[0030] Furthermore, various different embodiments of the present invention can be combined in any way, as long as they do not violate the spirit of the present invention, they should also be regarded as the content disclosed by the present invention.
Claims
1. A fermentation method employing "double irradiation sterilization", characterized in that, The method includes the following steps: Irradiation devices using accelerators (typically 1-10 MeV) or isotope radiation sources (such as cobalt-60 gamma sources) are used to intervene in the fermentation process; (1) Before fermentation, the fermentation raw materials are irradiated to sterilize them, killing miscellaneous bacteria and bacteriophages to achieve the required sterilization standards. The irradiation dose is usually selected as 1-5 KGy, and the specific irradiation dose parameter is selected according to the fermentation product; (2) After the fermentation raw materials are sterilized by irradiation, an appropriate amount of beneficial microbial strains for specific processes are added, and fermentation is carried out according to conventional fermentation processes; (3) After fermentation is complete, the mature fermented product is sterilized by irradiation to stop fermentation and extend the shelf life of the mature fermented product. The irradiation dose is usually selected as 1-5 KGy, and the specific irradiation dose parameter is selected according to the fermented product.
2. The method according to claim 1, characterized in that, The irradiation sterilization in steps (1) and (3) includes any one or a combination of two of the following: an irradiation device consisting of an accelerator or an isotope radiation source (such as a cobalt-60 gamma source).
3. The method according to claim 1, characterized in that, The fermentation raw materials mentioned in steps (1), (2), and (3) include, but are not limited to, fermentation raw materials such as tobacco leaves, tea leaves, grains, and feed.