Anti-aging compound additive for pets

Abstract

The invention relates to the technical field of pet food, in particular to a compound additive for pet aging resistance. The composite additive for pet aging resistance is prepared from the following raw materials in parts by weight: 20 to 25 parts of nicotinamide, 10 to 15 parts of cotinus coggygria extract, 20 to 25 parts of wheat germ extract, 65 to 100 parts of hydrolyzed fish protein powder and 1 to 2 parts of natural xanthophyll. The composite additive disclosed by the invention has the synergistic anti-aging effects of restarting energy, eliminating aging, remodeling autophagy and the like, and also has the effects of regulating immunity, resisting inflammation, protecting eyes, protecting joints and improving fur.

Description

Technical Field

[0001] This invention relates to the field of pet food technology, and in particular to a compound additive for pet anti-aging. Background Technology

[0002] As pets age, they experience a multi-system decline, including cognitive decline, joint degeneration, coat discoloration, and weakened immunity, making it one of the major problems affecting pet ownership. Existing pet anti-aging products suffer from the following drawbacks: 1. Limited Functionality: Most mainstream solutions target only one or two of the following: joint health (chondroitin, glucosamine), coat health (fish oil), or antioxidants (vitamin E). Owners of older pets often need to purchase 3-5 different formulations, leading to complex feeding practices and low compliance. 2. Lack of Synergistic Effects: Simple physical mixing can result in antagonistic interactions between components. For example, high doses of nicotinamide can inhibit sirtuin activity, and excessive antioxidants can block autophagy signals. Furthermore, simple physical mixing doesn't consider palatability, leading to high rates of food rejection in older pets. 3. Incomplete Product Evaluation Metrics: Most studies only measure serum antioxidant levels, lacking systematic validation of core aging markers (SA-β-gal, p16 / p21, lysosomal function). 4. Currently, most academic formulations use ingredients not included in the "List of Feed Additives," making commercialization impossible.

[0003] It is evident that existing technologies still need improvement and enhancement. Summary of the Invention

[0004] In view of the shortcomings of the prior art, the purpose of this invention is to provide a compound additive for pet anti-aging, which aims to improve the anti-aging performance of pet anti-aging products.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: A compound additive for pet anti-aging, by weight, comprises the following raw materials: 20-25 parts nicotinamide, 10-15 parts smoke tree extract, 20-25 parts wheat germ extract, 65-100 parts hydrolyzed fish protein powder, and 1-2 parts natural lutein.

[0006] The compound additive for pet anti-aging includes, by weight, the following raw materials: 5-10 parts sodium chondroitin sulfate and 10-20 parts egg yolk lecithin.

[0007] The compound additives for pet anti-aging play a role in the preparation of products that improve pet coat condition, skin elasticity, and mobility.

[0008] The role of the compound additive for pet anti-aging in the preparation of products that reduce serum β-galactosidase levels.

[0009] The role of the compound additive for pet anti-aging in the preparation of products that reduce serum p21 gene content.

[0010] Beneficial effects: This invention provides a compound additive for pet anti-aging. The compound additive uses nicotinamide, smoke tree extract and wheat germ extract as the main functional components, combined with other components such as natural lutein and chondroitin sulfate, to form a synergistic anti-aging effect in three aspects: "energy restart, aging removal and autophagy remodeling". It also has the effects of regulating immunity, anti-inflammation, eye protection and joint and fur protection. Attached Figure Description

[0011] Figure 1 This is a heatmap of mouse body weight.

[0012] Figure 2 The value represents the β-galactosidase content in mouse serum.

[0013] Figure 3 The p21 gene content in mouse serum. Detailed Implementation

[0014] This invention provides a compound additive for anti-aging in pets. To make the objectives, technical solutions, and effects of this invention clearer and more explicit, the invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only for explaining the invention and are not intended to limit the invention.

[0015] A compound additive for pet anti-aging, comprising, by weight, the following raw materials: 20-25 parts nicotinamide, 10-15 parts smoke tree extract, 20-25 parts wheat germ extract, 65-100 parts hydrolyzed fish protein powder, and 1-2 parts natural lutein. Of these components, nicotinamide is an NAD+ derivative. + The precursor to NAD+ can activate energy metabolism and the Sirtuin pathway; + Nicotinamide is an essential coenzyme for sirtuin (a silencing signaling factor), and supplementation with nicotinamide can increase intracellular NAD. +The levels of these substances activate subtypes such as SIRT1 and SIRT3. SIRT1 can deacetylate aging-related proteins such as p53 and FOXO, inhibiting cellular aging pathways. SIRT3 can repair mitochondrial damage, scavenge reactive oxygen species (ROS), and maintain mitochondrial functional stability, thus delaying cellular and organismal aging at the core level. Smokewort extract is rich in flavonoids such as fisetin, which can directly scavenge ROS and activate the Nrf2 pathway, enhancing the activity of endogenous antioxidant enzymes such as SOD and GSH-Px, thereby inhibiting the vicious cycle of oxidative stress-inflammation-aging and playing an anti-aging role along the "scavenging axis." Wheat germ extract is rich in vitamin E, linoleic acid, phytosterols, and glutathione precursors, which can activate the AMPK / mTOR signaling axis, induce autophagy, clear damaged proteins and mitochondria, maintain cellular homeostasis, and thus delay aging along the "autophagy axis." Natural lutein can effectively scavenge reactive oxygen species, resist oxidative stress damage, and play an anti-aging role. Hydrolyzed fish protein powder is a product of fish protein hydrolyzed by proteases. It contains small molecule peptides, free amino acids, polysaccharides and minerals. It can improve the palatability of food while providing pets with efficient nutritional supplementation. In addition, the small molecule peptides and free amino acids in the hydrolysate can be quickly absorbed by pets.

[0016] Preferably, the compound additive for pet anti-aging further comprises the following raw materials: 5-10 parts sodium chondroitin sulfate and 10-20 parts egg yolk lecithin. Sodium chondroitin sulfate is a natural glycosaminoglycan (GAG) widely found in connective tissues such as joint cartilage, intervertebral discs, and tendons in animals. Administering sodium chondroitin sulfate to pets can protect their joint cartilage, delay cartilage degeneration, and improve osteoarthritis. Egg yolk lecithin contains phosphatidylcholine, a precursor to the neurotransmitter acetylcholine, which can improve age-related cognitive impairment in older dogs and cats and enhance their responsiveness. Furthermore, the unsaturated fatty acids in egg yolk lecithin can improve pet coat health.

[0017] The composite additive of the present invention avoids problems such as high dose of nicotinamide inhibiting Sirtuin activity and excessive antioxidant blocking autophagy signals by rationally matching the dosage of each component. At the same time, it is combined with other components with different functions to improve the overall anti-aging performance.

[0018] The following animal experiments further illustrate the invention.

[0019] I. Laboratory Animals Male ICR mice, 8 weeks old, were housed in an indoor environment with a room temperature of 18-22℃ and a relative humidity of 50%-60%.

[0020] II. Grouping and Treatment of Experimental Mice The mice were divided into three groups: a control group, a model group, and a drug treatment group (comprising nine subgroups), with ten mice in each group. The mice in the model group and the drug treatment group were constructed as D-galactose-aged mice. All mice were housed in separate cages within the same room and provided with free access to food (basic diet) and water.

[0021] Construction of a D-galactose aging mouse model: Mice in the model group and drug treatment group were subcutaneously injected with 5% sterile D-galactose at the back of the neck at a dose of 500 mg / kg•day, while mice in the blank group were injected with an equal volume of physiological saline. The injections were repeated for 90 days to construct a mouse aging model.

[0022] Daily observation of mice revealed that the model group and drug treatment group mice exhibited dull fur, weight loss, and sluggish movement, indicating that mice injected subcutaneously with D-galactose showed a series of aging phenomena. In contrast, the control group mice had shiny fur and were agile, indicating that the modeling was successful.

[0023] After 60 days of injection of D-galactose, the drug treatment groups (9 subgroups in total) began gavage administration. Each subgroup in the drug treatment group received daily doses calculated based on the mouse's body weight, according to the additive dosages in Examples 1-3 and Comparative Examples 1-6, dissolved in an appropriate amount of pure water, and then administered the drug via gavage. The blank control group and the model group received the same volume of physiological saline via gavage for 30 consecutive days.

[0024] The amounts of additives used in Examples 1-3 and Comparative Examples 1-6 are as follows: Example 1 Nicotinamide 20mg / kg, Smoke Tree Extract 10mg / kg, Wheat Germ Extract 20mg / kg, Hydrolyzed Fish Protein Powder 65mg / kg, Natural Lutein 1mg / kg.

[0025] Example 2 Nicotinamide 25mg / kg, Smoke Tree Extract 15mg / kg, Wheat Germ Extract 25mg / kg, Hydrolyzed Fish Protein Powder 100mg / kg, Chondroitin Sulfate Sodium 10mg / kg, Egg Yolk Lecithin 20mg / kg, Natural Lutein 2mg / kg.

[0026] Example 3 Nicotinamide 20mg / kg, Smoke Tree Extract 10mg / kg, Wheat Germ Extract 20mg / kg, Hydrolyzed Fish Protein Powder 65mg / kg, Chondroitin Sulfate Sodium 5mg / kg, Egg Yolk Lecithin 10mg / kg, Natural Lutein 1mg / kg.

[0027] Comparative Example 1 Smoke tree extract 10mg / kg, wheat germ extract 20mg / kg, hydrolyzed fish protein powder 65mg / kg, sodium chondroitin sulfate 5mg / kg, egg yolk lecithin 10mg / kg, natural lutein 1mg / kg.

[0028] Comparative Example 2 Nicotinamide 20mg / kg, wheat germ extract 20mg / kg, hydrolyzed fish protein powder 65mg / kg, sodium chondroitin sulfate 5mg / kg, egg yolk lecithin 10mg / kg, natural lutein 1mg / kg.

[0029] Comparative Example 3 Nicotinamide 20mg / kg, Smoke Tree Extract 10mg / kg, Hydrolyzed Fish Protein Powder 65mg / kg, Chondroitin Sulfate Sodium 5mg / kg, Egg Yolk Lecithin 10mg / kg, Natural Lutein 1mg / kg.

[0030] Comparative Example 4 Nicotinamide 10mg / kg, Smoke Tree Extract 5mg / kg, Wheat Germ Extract 10mg / kg, Hydrolyzed Fish Protein Powder 30mg / kg, Chondroitin Sulfate Sodium 3mg / kg, Egg Yolk Lecithin 5mg / kg, Natural Lutein 0.5mg / kg.

[0031] Comparative Example 5 Glucosamine 10 mg / kg, chondroitin sulfate 5 mg / kg, lecithin 10 mg / kg.

[0032] Comparative Example 6 NMN (β-nicotinamide mononucleotide) 30mg / kg, resveratrol 20mg / kg, astaxanthin 10mg / kg.

[0033] III. Mouse Treatment and Index Measurement Mice were weighed and their weights recorded before modeling, after modeling, and after drug administration.

[0034] Aging is the process by which the body's constituent substances, tissue structure, and physiological functions decline with age. Upregulation of cell cycle inhibitors such as p21 and p16, increased levels of reactive oxygen species (ROS), and the accumulation of aging-related β-galactosidase (SA-β-gal) are all typical characteristics of aging. Therefore, β-galactosidase and p21 were chosen as indicators for aging detection.

[0035] After the final gavage, mice in each group were fasted but allowed free access to water. Twenty-four hours later, blood was collected from the orbital sinus of each mouse, and the mice were euthanized. The blood was centrifuged at 3000 rpm for 10 minutes, and the supernatant was collected. The levels of β-galactosidase and p21 gene in mouse serum were detected by enzyme-linked immunosorbent assay (ELISA). The assay method was performed according to the kit instructions.

[0036] IV. Data Processing SPSS 10.0 statistical software was used to perform analysis of variance on the relevant data. The parameters in the table are expressed as X±s. The significance level of the difference is P<0.05(*), the extremely significant level of the difference is P<0.01(**), and P<0.001(***).

[0037] V. Experimental Results 1. Results of mouse general condition score Table 1

[0038] Throughout the experiment, the general condition of the mice was observed regularly. Mice that were successfully modeled showed decreased appetite, slower weight gain, rough and messy fur, thin body shape, and sluggish movement. Mice that were not modeled showed a strong appetite, continuous weight gain, shiny fur, and agile movement. After drug treatment, all mice showed varying degrees of improvement, with increased appetite and weight gain. Overall, the mice in the drug treatment group showed significantly better vitality.

[0039] 2. Weight Record Table 2

[0040] Figure 1 For weight heatmap, Figure 1 In Table 2, modeling D1 represents day 1 of modeling, modeling D50 represents day 50 of modeling, and drug administration D30 represents day 30 after drug administration.

[0041] From Table 2 and Figure 1 The results showed that the initial weights of the mice were similar. The weights of the mice after D-galactose modeling were less than those of the control group. After 30 days of treatment, the mice in the drug treatment group were all heavier than those in the model group. The weights of the mice in Examples 1-3 and Comparative Example 6 increased significantly.

[0042] 3. Serum β-galactosidase content in mice Figure 2 The results show the levels of β-galactosidase in mouse serum. Figure 2 As can be seen, the model group showed a significantly higher level than the control group, exhibiting a substantial difference. Examples 1-3 and Comparative Examples 1-6 all showed a decrease compared to the model group, with Comparative Example 3 showing a significant difference, Comparative Examples 1 and 2 showing highly significant differences, and Examples 1, 2, 3, and Comparative Example 6 showing very significant differences. Although the formulation of Comparative Example 6 showed better results, its ingredients are not currently recorded in the "Feed Additives / Raw Materials Catalog," thus preventing its commercialization. Compared to Examples 3 and 2, Comparative Example 4 used less of each component. The results showed that the serum β-galactosidase content in the treated mice was significantly higher, with no significant difference from the model group, indicating that appropriate dosage is one of the key factors for efficacy.

[0043] 4. p21 gene content in mouse serum Figure 3 The results show the levels of β-galactosidase in mouse serum. Figure 3 It can be seen that the model group is much higher than the blank group, showing a very significant difference; Examples 1-3 and Comparative Examples 1-6 are all lower than the model group, and Comparative Examples 1, 2, 3 and 6 show significant differences, while Examples 1-3 show extremely significant differences.

[0044] In summary, the experimental results based on the D-galactose-induced mouse aging model demonstrate that a four-axis synergistic intervention centered on nicotinamide, smoke tree extract, wheat germ extract, chondroitin sulfate sodium, and egg yolk lecithin can significantly reverse aging phenotypes. Among these, the formulation used in Example 3 showed the best overall anti-aging effect, with its general condition score returning to the level of the control group, body weight increasing by 11.8% compared to the model group, and serum β-galactosidase and p21 gene levels decreasing by 17.92% (P < 0.001) and 14.65% (P < 0.01), respectively. The effect was comparable to that of Comparative Example 6 (the academic benchmark formulation). Furthermore, all ingredients in Example 3 were selected from the "Feed Additives / Raw Material Catalog," meeting market requirements.

[0045] Comparative Examples 1-3, lacking any one axis, all showed a significant decrease in anti-aging efficacy, suggesting that multi-axis synergy is key to achieving a comprehensive improvement. Comparative Example 5, based on a commercially available product formula, only achieved moderate improvement; compared to this, the comprehensive advantages of this solution are further verified. The composite additive formula of this invention corresponds to Examples 1-3 and possesses the same technical effects.

[0046] It is understood that those skilled in the art can make equivalent substitutions or modifications to the technical solution and inventive concept of the present invention, and all such substitutions or modifications should fall within the protection scope of the appended claims.

Claims

1. A composite additive for anti-aging of pets, characterized in that, The preparation raw materials include, by weight fraction, 20-25 parts of nicotinamide, 10-15 parts of cotinus coggygria extract, 20-25 parts of wheat germ extract, 65-100 parts of hydrolyzed fish protein powder, and 1-2 parts of natural lutein.

2. The additive for anti-aging of pets according to claim 1, characterized in that, The preparation raw materials further include, by weight fraction, 5-10 parts of sodium chondroitin sulfate and 10-20 parts of egg yolk lecithin.

3. The additive for anti-aging of pets according to claim 1, characterized in that, The preparation raw materials include, by weight fraction, 20 parts of nicotinamide, 10 parts of cotinus coggygria extract, 20 parts of wheat germ extract, 65 parts of hydrolyzed fish protein powder, and 1 part of natural lutein.

4. The additive for anti-aging of pets according to claim 1, characterized in that, The preparation raw materials include, by weight fraction, 20 parts of nicotinamide, 10 parts of cotinus coggygria extract, 20 parts of wheat germ extract, 65 parts of hydrolyzed fish protein powder, 1 part of natural lutein, 2 parts of natural lutein, 5 parts of sodium chondroitin sulfate, and 10 parts of egg yolk lecithin.

5. The additive for anti-aging of pets according to claim 1, characterized in that, The preparation raw materials include, by weight fraction, 25 parts of nicotinamide, 15 parts of cotinus coggygria extract, 25 parts of wheat germ extract, 100 parts of hydrolyzed fish protein powder, 2 parts of natural lutein, 10 parts of sodium chondroitin sulfate, and 20 parts of egg yolk lecithin.

6. The use of the compound additive for anti-aging of pets according to any one of claims 1-5 in the preparation of a product for improving the hair condition, skin elasticity, and activity of pets.

7. The use of the compound additive for anti-aging of pets according to any one of claims 1-5 in the preparation of a product for reducing the serum β-galactosidase content.

8. The use of the compound additive for anti-aging of pets according to any one of claims 1-5 in the preparation of a product for reducing the serum p21 gene content.

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