Medicinal and edible organic selenium compound trace element nutritional oral liquid and preparation method thereof

Abstract

The invention provides a medicinal and edible organic selenium compound trace element nutritional oral liquid and a preparation method thereof, the oral liquid is prepared by taking a plurality of medicinal and edible traditional Chinese medicines as the basis, matching with compound trace elements and plant peptides, and performing multi-step extraction and accurate blending with specific hydrogen-rich water. The product has the effects of clearing away heat and toxic materials and nourishing yin and moistening lung of traditional Chinese medicines, has the dual functions of protecting liver by selenium and zinc and enhancing immunity by plant peptide and hydrogen element, is free of harmful chemical additives, high in safety and strong in stability, and solves the problem that the existing product cannot synchronously provide nutrients of organic selenium, hydrogen, strontium and titanium elements and plant peptide; the traditional Chinese medicine composition is suitable for fatigue recovery in sports or replacement of strong wine, can fill the market blank, and has popularization value.

Description

Technical Field

[0001] This invention relates to the field of health product technology, specifically to an organic selenium complex trace element oral liquid that is both medicinal and edible, and its preparation method. Background Technology

[0002] Trace elements are key substances for maintaining normal physiological functions in the human body, and their deficiency or imbalance has become a significant health issue affecting modern populations. In 1973, the World Health Organization (WHO) explicitly listed selenium and zinc as essential trace elements for animal life. In 1994, my country promulgated GB14880-1994, the "Standards for the Use of Food Fortifiers," which allowed the addition of selenium and zinc to food. In 2003, the U.S. Food and Drug Administration (FDA) further approved selenium as an anticancer agent, allowing selenium supplements to be labeled with anticancer properties, demonstrating the core value of these two elements for human health.

[0003] Selenium, acting as a "scavenger" in the human body, is an active component of glutathione peroxidase and various free radical scavenging enzymes. It can effectively prevent and treat liver disease, cancer, cardiovascular and cerebrovascular diseases, and digestive tract diseases. It also regulates the body's immunity, repairs damaged cells, and plays a detoxifying role. Zinc participates in the formation of over 200 human enzymes and is a core element for maintaining the function of immune cells and organs, crucial for growth, development, metabolism, and other life activities. However, about two-thirds of my country is selenium-deficient, and people lose significant amounts of selenium and zinc through sweat and urine during exercise, travel, physical labor, and alcohol consumption. Furthermore, the highly refined nature of modern diets further exacerbates selenium and zinc deficiency in urban populations, directly leading to weakened immunity. In recent years, the incidence of the four major diseases threatening human health—liver disease, cancer, cardiovascular and cerebrovascular diseases, and digestive tract diseases—has been rising annually, making selenium and zinc supplementation an urgent need to improve public health.

[0004] Besides selenium and zinc, the health benefits of hydrogen, strontium, titanium, and peptides are also gaining attention. Hydrogen, when present in hydrogen-rich water, can effectively scavenge reactive oxygen species such as hydroxyl radicals, reducing oxidative damage to immune cells and helping to enhance immune function. Strontium participates in bone formation and promotes calcium absorption, indirectly maintaining the physiological stability required by the immune system. Titanium, when used in the form of food-grade organic acid titanium or titanium extracted from natural raw materials, is highly compatible with traditional Chinese medicine systems and can help improve the dissolution rate of effective components in traditional Chinese medicine; its safety in the food sector has been verified. Regarding peptides, plant peptides are easily digested and absorbed and can stimulate immune cell activity; macromolecular peptides excel in anti-fatigue and tissue repair. The synergy between these two can create a dual effect of "nutritional supplementation + functional enhancement," and their wide availability and controllable cost make them suitable for large-scale application in health product research and development.

[0005] From the current market perspective, most selenium and zinc supplements are concentrated in the pharmaceutical or single-function health food sectors. Compound oral liquids that combine traditional Chinese medicine conditioning, multi-element supplementation, peptide nutrition, and immune-boosting functions are extremely scarce. Existing herbal tea products, such as Wanglaoji, while primarily marketed for their heat-clearing properties, contain some ingredients previously banned by the Ministry of Health (such as Prunella vulgaris), failing to comply with regulations regarding the homology of food and medicine. Furthermore, these products generally lack the combined addition of selenium, zinc, hydrogen, strontium, titanium, and peptides, failing to meet the comprehensive needs of consumers for heat-clearing and detoxification, immune enhancement, and nutritional supplementation. In addition, traditional trace element supplements are mostly single-ingredient fortifications, making it difficult to achieve the synergistic effects of traditional Chinese medicine with trace elements and peptides. Some products also suffer from insufficient compliance with raw material regulations, limited functionality, and poor stability, failing to meet the modern consumer demand for multifunctional, safe, and easily absorbed health supplements.

[0006] In summary, the development of a compound trace element oral liquid with organic selenium as its core, combined with organic zinc, strontium, and titanium elements, along with plant peptides and macromolecular peptides, and based on traditional Chinese medicine of the same origin as food, can achieve multiple functions such as clearing heat and detoxifying, nourishing yin and moistening the lungs, supplementing trace elements, and enhancing immunity and anti-fatigue. It is suitable for relieving fatigue and replenishing water after exercise and physical labor, and can also serve as a healthy alternative to strong alcohol. It provides a new solution for improving the population's trace element deficiency and enhancing health, and has significant market value and social significance. Summary of the Invention

[0007] Purpose of the invention: The purpose of this invention is to provide an oral liquid containing organic selenium complex trace elements that is both medicinal and edible, and its preparation method. By screening medicinal and edible herbs, combining them with compliant organic selenium, organic zinc and other trace elements, and integrating hydrogen-rich water and complex peptides, the oral liquid is prepared through a scientific process to achieve the synergistic effects of traditional Chinese medicine conditioning, multi-element supplementation, immune enhancement and anti-fatigue. It ensures that there are no harmful chemical additives, and provides a safe and efficient health product for relieving fatigue during exercise and replacing strong alcohol. It solves the problems of existing products that cannot simultaneously provide organic selenium, hydrogen, strontium, titanium and dual nutrition of plant peptides and macromolecular peptides, and cannot simultaneously take into account the heat-clearing and detoxifying, yin-nourishing and lung-moistening and immune-regulating functions of traditional Chinese medicine. Some products also have problems such as non-compliant raw materials and poor stability.

[0008] The technical solution of the present invention: An oral liquid containing organic selenium complex trace elements, which is both medicinal and edible, is prepared from the following raw materials: 180-220g honeysuckle, 280-320g lily, 380-420g chrysanthemum, 280-320g polygonatum, 280-320g jujube, 180-220g dried plum, 280-320g kudzu root, 380-420g peppermint, 50-300g food-grade organic acid titanium, 1-3kg plant peptides, 0.3-1kg macromolecular peptides, 0.001-0.02g organic selenium, 0.1-2g organic zinc, 0.01-0.3g strontium citrate, 1-10kg honey, 20-100g citric acid, with the remainder being hydrogen-rich water, and a total mass of 100kg.

[0009] In some embodiments of the present invention, the food organic acid titanium is one or more of titanium citrate, titanium malate, and titanium lactate; the macromolecular peptide is one or more of sturgeon peptide, whey protein peptide, and collagen peptide.

[0010] In some embodiments of the present invention, the honeysuckle can be replaced with forsythia, or isatis root; the chrysanthemum can be replaced with mulberry leaves or cassia seeds.

[0011] In some embodiments of the present invention, the organic selenium is one or more of selenomethionine, selenocysteine, and selenized carrageenan, and the organic zinc is one or more of zinc gluconate, zinc glycinate, and zinc citrate; the hydrogen-rich water is prepared using micro-nano bubble technology, and the hydrogen solubility is 1.2-1.6 mg / L.

[0012] In some embodiments of the present invention, the plant peptide is at least two of soybean peptide, corn peptide, wheat peptide, pea peptide, and peanut peptide.

[0013] This invention provides a method for preparing an organic selenium complex trace element nutritional oral liquid that is both medicinal and edible, characterized by comprising the following steps: S1: Preparation of Chinese herbal extract: Mix honeysuckle, lily, chrysanthemum, polygonatum, jujube, dried plum, kudzu root and mint, add 25-45 times the weight of hydrogen-rich water, soak for several hours, then decoct for 40-50 minutes, filter to obtain residue and filtrate No. 1. S2: Secondary extraction: Add food organic acid titanium and hydrogen-rich water to the residue after boiling in S1, boil for 40-50 minutes, and filter to obtain residue and filtrate No. 2. S3: Three-stage extraction: Add hydrogen-rich water to the residue after boiling in S2, boil for 40-50 minutes, and filter to obtain residue and filtrate No. 3. S4: Four extractions: Add hydrogen-rich water to the residue after boiling in S3, boil for 40-50 minutes, and filter to obtain residue and filtrate No. 4. S5: Mixing and blending: Combine filtrates 1, 2, 3, and 4, add plant peptides, macromolecular peptides, organic selenium, organic zinc, and strontium citrate, stir for 15-20 minutes, then add honey and citric acid, stir evenly, add hydrogen-rich water to the total mass, adjust the pH to 6-7 with 1 mol / L sodium bicarbonate, and stir for 30-45 minutes; S6: Filter the pH-adjusted mixture from S5 through a filter membrane, then pasteurize it at low temperature for 30-35 minutes, transfer it to a sterile storage tank, aseptically fill and seal it, pasteurize it again for 20-25 minutes, and finally spray code and package it to obtain an organic selenium compound trace element nutritional oral liquid that is both medicinal and edible.

[0014] In some embodiments of the present invention, the soaking time in S1 is 1-1.5 hours, and the decocting temperature is 60-70°C.

[0015] In some embodiments of the present invention, the amount of hydrogen-rich water added in S2, S3, and S4 is 10-20 times the mass of the residue in S1, S2, and S3, respectively; and the decoction temperature in S2, S3, and S4 is 60-70°C.

[0016] In some embodiments of the present invention, the plant peptide in S5 is a mixture of soybean peptide and corn peptide in a mass ratio of 1:0.8.

[0017] In some embodiments of the present invention, the filter membrane pore size in S7 is 0.22-0.45 μm, and the low-temperature pasteurization temperature is 60-65℃.

[0018] In some embodiments of the present invention, the hydrogen-rich water is used throughout the preparation process and is stored at a temperature of 2-8°C.

[0019] Beneficial effects: 1. The oral liquid prepared by this invention has strong synergistic function. The Chinese medicine ingredients achieve heat-clearing and detoxifying, nourishing yin and moistening the lungs, organic selenium and organic zinc synergistically protect the liver, and the compound peptides, hydrogen-rich water and strontium elements form a triple effect of immune enhancement, anti-fatigue and bone protection, which solves the functional limitations of single products and can be adapted to scenarios such as exercise fatigue relief, daily health care and strong liquor replacement. 2. The raw materials are compliant and safe, with no health risks. Many Chinese medicines are listed in the catalog of food and medicine homology. Organic selenium, organic zinc, food-grade titanium and other elements meet the GB14880 standard. The pH adjustment (6-7) and low-temperature sterilization (60-65℃) prevent the precipitation or denaturation of components, ensuring both safety and stability. 3. The four-stage extraction process fully preserves the effective components of traditional Chinese medicine, and the use of hydrogen-rich water throughout the process ensures antioxidant activity. The combination of complex peptides improves protein digestibility. Compared with traditional herbal teas or single trace element drinks, the product has a higher nutritional density and more comprehensive functions, making it significantly more competitive in the market. 4. High application flexibility, adaptable to different needs. Honeysuckle can be replaced with forsythia, chrysanthemum can be replaced with mulberry leaves, selenized carrageenan can be selected for organic selenium, zinc citrate can be selected for organic zinc, and whey protein peptides can be selected for macromolecular peptides. All of these support multiple product substitutions. Furthermore, customized formulas with high peptide nutrition and low cost can be achieved by adjusting the proportion of raw materials, adapting to different consumer groups and market positioning. Detailed Implementation

[0020] The present invention will be described below with reference to specific implementation schemes. It should be noted that the experimental examples below are illustrative of the present invention and are used only to illustrate the invention, not to limit it. Other combinations and various modifications within the scope of the present invention can be made without departing from its spirit or scope.

[0021] Unless otherwise specified, all chemical reagents used in this invention are commercially available analytical grade reagents.

[0022] Example 1 Raw materials (total mass 100kg): Ingredients: 200g honeysuckle, 300g lily bulb, 400g chrysanthemum, 300g polygonatum, 300g jujube, 200g dried plum, 300g kudzu root, 400g mint, 150g titanium citrate, 1kg soybean peptide, 0.8kg corn peptide (1:0.8), 0.5kg sturgeon peptide, 0.01g selenomethionine, 1.4g zinc gluconate, 0.15g strontium citrate, 5kg honey, 60g citric acid, with the remainder being hydrogen-rich water (hydrogen solubility 1.4mg / L). Preparation method of organic selenium complex trace element oral liquid with medicinal and edible properties: S1: Preparation of Chinese herbal extract: Mix honeysuckle, lily, chrysanthemum, polygonatum, jujube, dried plum, kudzu root and mint, add 30 times the mass of hydrogen-rich water, soak for 1 hour, decoct at 65℃ for 45 minutes, filter to obtain filtrate No. 1 and residue No. 1. S2: Secondary extraction: Add titanium citrate and 15 times the mass of hydrogen-rich water to residue 1, and boil at 65°C for 45 minutes to obtain filtrate 2 and residue 2. S3: Three extractions: Add 15 times the mass of hydrogen-rich water to residue No. 2, boil at 65℃ for 45 minutes, and filter to obtain filtrate No. 3 and residue No. 3. S4: Four extractions: Add 15 times the mass of hydrogen-rich water to residue No. 3, boil at 65℃ for 45 minutes, and filter to obtain filtrate No. 4 and residue No. 4. S5: Mixing and Blending: Combine filtrates 1, 2, 3, and 4, add soybean peptide, corn peptide, sturgeon peptide, selenomethionine, zinc gluconate, and strontium citrate, stir for 18 minutes, then add honey and citric acid, stir evenly, add hydrogen-rich water to 100 kg, adjust the pH to 6.5 with 1 mol / L sodium bicarbonate, and stir for 35 minutes; S6: The pH-adjusted mixture from S5 is filtered through a 0.3μm filter membrane, then pasteurized at 62℃ for 32 minutes, transferred to a sterile storage tank, aseptically filled and sealed, and then pasteurized again at 62℃ for 22 minutes. Finally, it is printed and packaged to obtain an organic selenium complex trace element nutritional oral liquid that is both medicinal and edible.

[0023] Example 2 Raw materials (total mass 100kg): Forsythia 220g, lily 320g, mulberry leaf 420g, polygonatum 320g, jujube 320g, dried plum 220g, kudzu root 320g, peppermint 420g, titanium lactate 180g, soybean peptide 1.5kg, corn peptide 1.5kg, sturgeon peptide 1kg, selenomethionine 0.02g, zinc gluconate 2g, strontium citrate 0.3g, honey 10kg, citric acid 100g, the remainder being hydrogen-rich water (1.6mg / L). Preparation method of organic selenium complex trace element oral liquid with medicinal and edible properties: S1: Preparation of Chinese herbal extract: Forsythia, lily, mulberry leaf, polygonatum, jujube, dried plum, kudzu root and mint are mixed, 30 times the weight of hydrogen-rich water is added, soaked for 1 hour, decocted at 65℃ for 45 minutes, and filtered to obtain filtrate No. 1 and residue No. 1. S2: Secondary extraction: Add titanium citrate and 15 times the mass of hydrogen-rich water to residue 1, and boil at 65°C for 45 minutes to obtain filtrate 2 and residue 2. S3: Three extractions: Add 15 times the mass of hydrogen-rich water to residue No. 2, boil at 65℃ for 45 minutes, and filter to obtain filtrate No. 3 and residue No. 3. S4: Four extractions: Add 15 times the mass of hydrogen-rich water to residue No. 3, boil at 65℃ for 45 minutes, and filter to obtain filtrate No. 4 and residue No. 4. S5: Mixing and Blending: Combine filtrates 1, 2, 3, and 4, add soybean peptide, corn peptide, sturgeon peptide, selenomethionine, zinc gluconate, and strontium citrate, stir for 18 minutes, then add honey and citric acid, stir evenly, add hydrogen-rich water to 100 kg, adjust the pH to 6.2 with 1 mol / L sodium bicarbonate, and stir for 35 minutes; S6: The pH-adjusted mixture from S5 is filtered through a 0.3μm filter membrane, then pasteurized at 62℃ for 32 minutes, transferred to a sterile storage tank, aseptically filled and sealed, and then pasteurized again at 62℃ for 22 minutes. Finally, it is printed and packaged to obtain an organic selenium complex trace element nutritional oral liquid that is both medicinal and edible.

[0024] Example 3 Raw materials (total mass 100kg): Honeysuckle 190g, lily bulb 290g, cassia seed 410g, polygonatum rhizome 290g, jujube 290g, dried plum 190g, kudzu root 290g, peppermint 410g, titanium citrate: titanium lactate = 1:1 (total 140g), corn peptide 1kg, pea peptide 0.8kg, collagen peptide 0.6kg, selenized carrageenan 0.012g, zinc citrate 1.5g, strontium citrate 0.18g, honey 6kg, citric acid 70g, the remainder is hydrogen-rich water (1.6mg / L); Preparation method of organic selenium complex trace element oral liquid with medicinal and edible properties: S1: Preparation of Chinese herbal extract: Mix honeysuckle, lily, cassia seed, polygonatum, jujube, dried plum, kudzu root and mint, add 30 times the weight of hydrogen-rich water, soak for 1 hour, decoct at 70℃ for 40 minutes, filter to obtain filtrate No. 1 and residue No. 1. S2: Secondary extraction: Add titanium citrate and 15 times the mass of hydrogen-rich water to residue 1, and boil at 65°C for 40 minutes to obtain filtrate 2 and residue 2. S3: Three extractions: Add 15 times the mass of hydrogen-rich water to residue No. 2, boil at 70℃ for 40 minutes, and filter to obtain filtrate No. 3 and residue No. 3. S4: Four extractions: Add 15 times the mass of hydrogen-rich water to residue No. 3, boil at 70℃ for 40 minutes, and filter to obtain filtrate No. 4 and residue No. 4. S5: Mixing and Blending: Combine filtrates 1, 2, 3, and 4, add soybean peptide, corn peptide, sturgeon peptide, selenomethionine, zinc gluconate, and strontium citrate, stir for 18 minutes, then add honey and citric acid, stir evenly, add hydrogen-rich water to 100 kg, adjust the pH to 6.5 with 1 mol / L sodium bicarbonate, and stir for 35 minutes; S6: The pH-adjusted mixture from S5 is filtered through a 0.45μm filter membrane, then pasteurized at 62℃ for 32 minutes, transferred to a sterile storage tank, aseptically filled and sealed, then pasteurized again at 62℃ for 22 minutes, and finally printed and packaged to obtain an organic selenium complex trace element nutritional oral liquid that is both medicinal and edible.

[0025] Example 4 Raw materials (total mass 100kg): Ingredients: 200g honeysuckle, 300g lily bulb, 400g chrysanthemum, 300g polygonatum, 300g jujube, 200g dried plum, 300g kudzu root, 400g mint, 150g titanium citrate, 1kg soybean peptide, 0.8kg corn peptide, 0.5kg sturgeon peptide, 0.01g selenomethionine, 1.4g zinc gluconate, 0.15g strontium citrate, 5kg honey, 60g citric acid, with the remainder being hydrogen-rich water (hydrogen solubility 1mg / L). The preparation method of the organic selenium complex trace element nutritional oral liquid, which is both food and medicine, is the same as in Example 1.

[0026] Example 5 Raw materials (total mass 100kg): Ingredients: 200g honeysuckle, 300g lily bulb, 400g chrysanthemum, 300g polygonatum, 300g jujube, 200g dried plum, 300g kudzu root, 400g mint, 150g titanium citrate, 1kg soybean peptide, 0.8kg corn peptide, 0.5kg sturgeon peptide, 0.01g selenomethionine, 1.4g zinc gluconate, 0.15g strontium citrate, 5kg honey, 60g citric acid, with the remainder being hydrogen-rich water (hydrogen solubility 1mg / L). The preparation method of the organic selenium compound trace element nutritional oral liquid that is both food and medicine is different from that of Example 1. The difference is that steps S3 and S4 are not used in Example 5, and the phrase "mixing and blending: combining filtrates 1, 2, 3, and 4" in step S5 is replaced with "mixing and blending: combining filtrates 1 and 2".

[0027] Comparative Example 1 Raw materials (total mass 100kg): Ingredients: 200g honeysuckle, 300g lily bulb, 400g chrysanthemum, 300g polygonatum, 300g jujube, 200g dried plum, 300g kudzu root, 400g mint, 150g titanium citrate, 1kg soybean peptide, 0.8kg corn peptide (1:0.8), 0.5kg sturgeon peptide, 0.01g selenomethionine, 1.4g zinc gluconate, 0.15g strontium citrate, 5kg honey, 60g citric acid, with the remainder being purified water. The preparation method of the organic selenium complex trace element nutritional oral liquid that is both food and medicine is basically the same as that in Example 1, except that the "hydrogen-rich water" in Comparative Example 1 is replaced with "purified water".

[0028] Comparative Example 2 Raw materials (total mass 100kg): Ingredients: 200g honeysuckle, 300g lily bulb, 400g chrysanthemum, 300g polygonatum, 300g jujube, 200g dried plum, 300g kudzu root, 400g mint, 150g titanium citrate, 0.5kg sturgeon peptide, 0.01g selenomethionine, 1.4g zinc gluconate, 0.15g strontium citrate, 5kg honey, 60g citric acid, with the remainder being hydrogen-rich water (hydrogen solubility 1.4mg / L). The preparation method of the organic selenium complex trace element nutritional oral liquid that is both food and medicine is the same as that in Example 1, except that soybean peptides and corn peptides are not added in step S5 of Comparative Example 2.

[0029] Comparative Example 3 Raw materials (total mass 100kg): Ingredients: 200g honeysuckle, 300g lily bulb, 400g chrysanthemum, 300g polygonatum, 300g jujube, 200g dried plum, 300g kudzu root, 400g mint, 150g titanium citrate, 1kg soybean peptide, 0.8kg corn peptide (1:0.8), 0.01g selenomethionine, 1.4g zinc gluconate, 0.15g strontium citrate, 5kg honey, 60g citric acid, with the remainder being hydrogen-rich water (hydrogen solubility 1.4mg / L). The preparation method of the organic selenium complex trace element nutritional oral liquid that is both food and medicine is the same as that in Example 1, except that sturgeon peptides are not added in step S5.

[0030] The following performance tests were conducted on Examples 1-5 and Comparative Examples 1-3 (a total of 8 groups of oral liquids, 100 kg prepared for each group, with a sample size of 500 mL / group): 1. Antioxidant performance test (hydroxyl radical scavenging rate) Standard referenced: DPPH method as specified in the "Technical Specifications for Inspection and Evaluation of Health Foods". Procedure: Prepare three experimental systems: blank group, control group, and sample group. Blank group: Add 5 mL of ethanol and 5 mL of 0.1 mmol / L DPPH ethanol solution and mix well. Control group: Add 5 mL of the oral liquid sample to be tested and 5 mL of ethanol and mix well. Sample group: Add 5 mL of the oral liquid sample to be tested and 5 mL of 0.1 mmol / L DPPH ethanol solution and mix well. Measure the absorbance at 517 nm using a UV spectrophotometer (model: UV-2600). Calculate the clearance rate (%) using the formula: [1 - (absorbance of sample group - absorbance of control group) / absorbance of blank group] × 100%, repeat three times and take the average value.

[0031] 2. Immunological activity test (macrophage phagocytosis rate) Experimental subjects: Kunming mice (weight 20±2g, 6 mice per group) Procedure: Extract mouse peritoneal macrophages and adjust the concentration to 1×10⁻⁶. 6 Cells / mL were seeded into 96-well plates (100 μL / well) and incubated at 37°C and 5% CO2 for 2 h. The supernatant was discarded, and 50 μL of sample dilution buffer (oral solution: physiological saline = 1:10) + 50 μL of 0.075% neutral red staining solution was added to each well. The cells were incubated for another 1 h. After washing three times with physiological saline, 100 μL of cell lysis buffer (acetic acid: ethanol = 1:1) was added, and the cells were shaken for 10 min. The absorbance at 540 nm was then measured, which is the absorbance of the sample group. The phagocytosis rate (%) was calculated according to the formula: (absorbance of sample group / absorbance of blank control group) × 100% (the blank control group is physiological saline).

[0032] 3. Trace element stability test (selenium / zinc retention rate) Standards followed: GB 5009.93-2017 (Selenium), GB 5009.14-2017 (Zinc) Operating procedures: Each group of samples was sealed and placed in a light-protected environment at 25℃. Samples were taken at 0 days and 180 days. Selenium content was measured using an atomic fluorescence spectrometer (model: AFS-930), and zinc content was measured using a flame atomic absorption spectrometer (model: AA-6300). The retention rate (%) was calculated using the formula: Retention rate (%) = (content at 180 days / initial content) × 100%. The results were repeated 3 times and the average value was taken.

[0033] 4. Hydrogen Residual Content Test Detection instrument: Gas chromatograph (Model: GC-2014, TCD detector, 5A molecular sieve column) Operating procedure: Take 10 mL of fresh sample and 10 mL of sample stored for 7 days, inject into a 20 mL headspace vial and seal. Keep the vial at 37℃ for 30 min. Detect the hydrogen concentration in the headspace gas and calculate using the formula: Residual amount (mg / L) = (Detection concentration × Headspace volume) / Sample volume (Headspace volume = 20 mL - 10 mL = 10 mL). Repeat 3 times and take the average value.

[0034] 5. Peptide digestibility test (in vitro simulated digestion model) Standard referenced: GB 5009.5-2016 (Kjeldahl method) Procedure: Take 5 mL of sample, add 10 mL of pH 1.2 artificial gastric juice (containing 3 g / L pepsin), shake at 37℃ for 2 h; adjust pH to 7.5, add 10 mL of pH 7.5 artificial intestinal juice (containing 1 g / L trypsin), and continue shaking for 4 h; centrifuge and collect the supernatant, determine the total nitrogen content before and after digestion using the Kjeldahl method, and calculate according to the formula: digestibility (%) = (soluble nitrogen content after digestion / total nitrogen content) × 100%, repeat 3 times and take the average value.

[0035] 6. Fatigue resistance test (mouse weight-bearing swimming test) Experimental subjects: Kunming mice (weight 20±2g, 10 mice per group) Procedure: Mice in each group were administered 0.2 mL / 10 g body weight of sample via gavage daily, while the control group was administered 0.2 mL / 10 g body weight of physiological saline via gavage for 7 consecutive days. One hour after the last gavage, mice were placed in a 25°C constant temperature water bath (30 cm deep) with a lead weight attached to their tails. Swimming time was recorded (from entry into the water until the head sinks to the bottom for 10 seconds without surfacing). The anti-fatigue time extension rate was calculated as follows: Extension rate (%) = (average swimming time of sample group - average swimming time of control group) / average swimming time of control group × 100%.

[0036] Table 1: Performance Test Results

[0037] The test results show that the organic selenium compound trace element nutritional oral liquid prepared in Example 1 of this invention exhibits balanced indicators across all aspects, stable antioxidant, immune-activating, and anti-fatigue effects, and good trace element retention after long-term storage. In its standard formula, the heat-clearing and detoxifying components of traditional Chinese medicine, the antioxidant effect of hydrogen-rich water, and the synergistic effect of peptides on immunity and anti-fatigue complement each other. Simultaneously, the four-stage extraction process fully dissolves the effective components in the traditional Chinese medicine and trace elements, constructing a stable functional system. The organic selenium compound trace element nutritional oral liquid prepared in Example 2 demonstrates the best overall performance, with stronger antioxidant capacity, more prominent anti-fatigue effects, and better long-term trace element retention. The formula adjustment utilizes traditional Chinese medicine with stronger heat-clearing and detoxifying effects. The formula contains a traditional Chinese medicine containing auxiliary antioxidant components; increasing the concentration of hydrogen-rich water further enhances the free radical scavenging ability; increasing the amount of peptides strengthens immune activation and fatigue repair; and combining it with organic acid titanium, which has good solubility in high-peptide systems, allows the functions of each component to be superimposed, achieving an overall performance improvement; the oral liquid of organic selenium compound trace element nutrition prepared in Example 3 exhibits outstanding stability, good titanium element solubility, and maintains good levels of antioxidant and immune activation functions. The traditional Chinese medicine in the formula is compatible with the characteristics of titanium element, and the use of compound organic acid titanium instead of single titanium avoids the solubility shortcomings of single titanium; at the same time, the combination of easily digestible peptides and peptides with repair effects not only retains the core functions but also improves the system compatibility, verifying the feasibility of raw material substitution. Feasibility; Compared with Example 1, Example 4 showed a significant decrease in the antioxidant performance of the oral liquid due to reduced hydrogen-rich water solubility, and the core antioxidant function failed to meet the standard. Only the immune and digestive related properties showed no significant change. This indicates that hydrogen is the core component of the oral liquid's antioxidant properties, and its reduced concentration directly reduces the free radical scavenging effect. It also proves that hydrogen-rich water needs to reach a certain concentration threshold to ensure qualified antioxidant function. Compared with Example 1, Example 5 only performed two extractions on the traditional Chinese medicine. The stability and core function of the oral liquid were significantly reduced, trace elements were easily lost, and the effective components of the traditional Chinese medicine were insufficient. It can be seen that two extractions cannot fully dissolve the active ingredients bound to trace elements in the traditional Chinese medicine, resulting in impaired overall function and stability. This further proves that four extractions are necessary to ensure the effectiveness of the oral liquid. The necessary process for component dissolution and maintaining functional stability; Comparative Example 1 compared with Example 1, Comparative Example 1 used purified water instead of hydrogen-rich water, and the core function of the oral liquid was completely lost, the antioxidant capacity was greatly reduced, and the immune activation effect was also significantly weakened. This is because without hydrogen, the oral liquid lost its antioxidant basis, and the activity of immune cells was reduced due to oxidative damage; even if the peptides and trace elements were normal, they could not form a synergistic effect of antioxidant and immune enhancement, which fully proves that hydrogen-rich water is the core technical feature of this invention; Comparative Example 2 compared with Example 1, Comparative Example 2 did not use plant peptides, but only retained large molecular peptides, and the digestibility and immune activation effect of the oral liquid were reduced. Plant peptides are small molecular peptides, which are easily digested and absorbed by the human body, and can synergistically activate immunity with trace elements such as selenium and zinc;When only macromolecular peptides are retained, not only is the absorption of peptides reduced, but the synergistic immune effect with trace elements is also lost. This demonstrates that plant peptides are key to achieving high digestibility and strong immune function. Comparing Comparative Example 3 with Example 1, without the addition of macromolecular sturgeon peptides, the anti-fatigue function of the oral liquid was completely ineffective; only immune and digestive performance remained unchanged. Macromolecular sturgeon peptides contain a special amino acid sequence that can enhance muscle endurance and reduce fatigue substances. Plant peptides, lacking this structure, while ensuring digestive and immune functions, cannot achieve an anti-fatigue effect. This verifies that peptides require a combination of plant peptides and macromolecular peptides to achieve multiple functions.

[0038] This invention can also be demonstrated through various other experimental examples. Without departing from the spirit and essence of this invention, those skilled in the art can make various corresponding changes and modifications according to this invention, but these corresponding changes and modifications should all fall within the protection scope of the appended claims.

Claims

1. A kind of organic selenium complex trace element nutritional oral liquid of food and drug, it is characterized in that, It is prepared from the following raw materials: honeysuckle 180-220g, lily 280-320g, chrysanthemum 380-420g, yellow 280-320g, jujube 280-320g, dark plum 180-220g, radix puerariae 280-320g, mint 380-420g, food organic acid titanium 50-300g, plant peptide 1-3kg, macromolecular peptide 0.3-1kg, organic selenium 0.001-0.02g, organic zinc 0.1-2g, strontium citrate 0.01-0.3g, honey 1-10kg, citric acid 20-100g, and the rest is hydrogen-rich water, with a total mass of 100kg.

2. The phisco-organic selenium complex microelemental nutrient oral liquid according to claim 1, characterized in that, The food organic acid titanium is a mixture of one or more of titanium citrate, titanium malate and titanium lactate; the macromolecular peptide is a mixture of one or more of sturgeon peptide, whey protein peptide and collagen peptide; the honeysuckle can be replaced by forsythia, forsythia or isatis root; the chrysanthemum can be replaced by mulberry leaf or cassia seed. 3.The symbiotic organic selenium and trace element nutrient oral liquid of claim 1, characterized in that, The organic selenium is a mixture of one or more of selenomethionine, selenocysteine and seleniumated carrageenan, and the organic zinc is a mixture of one or more of zinc gluconate, zinc glycinate and zinc citrate; the hydrogen-rich water is prepared by micro-nano bubble technology, with a hydrogen solubility of 1.2-1.6mg / L.

4. The phoecological organic selenium and microelement nutrient oral liquid according to claim 1, characterized in that, The plant peptide is at least two of soybean peptide, corn peptide, wheat peptide, pea peptide and peanut peptide.

5. The preparation method of the organic selenium compound microelement oral liquid of the same source of medicine and food according to any one of claims 1-4, characterized in that, It comprises the following steps: S1: Chinese medicine extract preparation: mix honeysuckle, lily, chrysanthemum, yellow, jujube, dark plum, radix puerariae and mint, add 25-45 times the mass of hydrogen-rich water, soak for several hours, then decoct for 40-50 minutes, filter to obtain residue and filtrate No.1; S2: secondary extraction: add food organic acid titanium and hydrogen-rich water to the residue after decoction in S1, decoct for 40-50 minutes, filter to obtain residue and filtrate No.2; S3: third extraction: add hydrogen-rich water to the residue after decoction in S2, decoct for 40-50 minutes, filter to obtain residue and filtrate No.3; S4: fourth extraction: add hydrogen-rich water to the residue after decoction in S3, decoct for 40-50 minutes, filter to obtain residue and filtrate No.4; S5: mixing and blending: combine filtrates No.1, 2, 3 and 4, add plant peptide, macromolecular peptide, organic selenium, organic zinc and strontium citrate, stir for 15-20 minutes, then add honey and citric acid, stir until uniform, add hydrogen-rich water to the total mass, adjust the pH to 6-7 with 1mol / L sodium bicarbonate, and stir for 30-45 minutes; S6: filter the mixture after adjusting the pH in S5 through a filter membrane, then perform low-temperature pasteurization for 30-35 minutes, transfer to a sterile storage tank, perform sterile filling and sealing, then perform pasteurization again for 20-25 minutes, finally spray code, package, and obtain the organic selenium compound trace element nutritional oral liquid with the same source of medicine and food.

6. The preparation method of the pharmaconutritional organic selenium complex trace element oral liquid according to claim 5, characterized in that, The soaking time in S1 is 1-1.5 hours, and the decoction temperature is 60-70℃.

7. The preparation method of the pharmaconutritional organic selenium complex trace element oral liquid according to claim 5, characterized in that, The hydrogen-rich water is added in S2, S3 and S4 in an amount of 10-20 times the mass of the residue in S1, S2 and S3 respectively; the decoction temperature in S2, S3 and S4 is 60-70℃.

8. The preparation method of the pharmaconutritional organic selenium complex microelement oral liquid according to claim 5, characterized in that, The plant peptide in S5 is a mixture of soybean peptide and corn peptide with a mass ratio of 1:0.

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9. The preparation method of the pharmaconutritional organic selenium complex microelement oral liquid according to claim 5, characterized in that, The filter membrane in S6 has a pore size of 0.22-0.45 microns, and the low-temperature pasteurization temperature is 60-65 DEG C.

10. The preparation method of the pharmaconutritional organic selenium complex microelement oral liquid according to claim 5, characterized in that, The hydrogen-rich water is used throughout the preparation process, and the storage temperature is 2-8 DEG C.

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