Method for evaluating maintenance quality of cigars
By capturing particulate matter and gaseous components of the mainstream cigar smoke and quantitatively detecting aroma components using GC/MS analysis, a sensory evaluation method was established. This solved the problem of difficulty in evaluating the quality of cigar maintenance and enabled scientific and precise control of the cigar maintenance process.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-30
- Publication Date
- 2026-03-31
AI Technical Summary
The lack of effective methods in the current technology to evaluate the changes and quality of aroma components during the maintenance of cigars makes it difficult to accurately control the quality of cigar maintenance.
By capturing particulate matter and gaseous components of the mainstream smoke of cigars, the content and proportion of alkaline, neutral and acidic aroma components are quantitatively detected by GC/MS analysis. A sensory evaluation method is established, and the sensory score is used to determine the maintenance quality of cigars.
This study provides a scientific method for evaluating the quality of cigar maintenance, predicting the sensory quality of cigars through aroma component data, providing a basis for precise cigar maintenance, and improving the scientific nature and precision of cigar quality control.
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Abstract
Description
Technical Field
[0001] This invention relates to the field of chemical testing technology for cigars, specifically to a method for evaluating the quality of cigar preservation. Background Technology
[0002] Cigars are tobacco products with unique aromas and cultural attributes. They are characterized by their rich and mellow aroma, strong strength, intense flavor, and low tar content, making them increasingly popular and well-received by consumers. Curing, as a crucial step in cigar manufacturing, directly determines sensory qualities such as style, aroma richness, and body. The synergistic effect of the chemical components in cigar smoke directly reflects sensory quality and style characteristics, making it a key factor in establishing the core quality of cigar products. Researching the aroma chemicals in cigars is of great significance for improving and monitoring cigar quality.
[0003] Currently, there is limited publicly available information on cigar curing, but the principles of cigar curing and aging are similar to those of cigar tobacco fermentation, which can provide a reference for research on cigar curing and aging. Under suitable temperature and humidity conditions, enzymatic reactions and microbial metabolism continuously occur inside the tobacco leaves in the cigar, promoting the degradation of macromolecules such as nicotine and proteins, and the production of small volatile aroma substances such as esters, aldehydes, and ketones.
[0004] Therefore, developing methods for analyzing the content of characteristic aroma components in cigars, analyzing the impact of long-term maintenance on the aroma components in cigars, and revealing the changes of various aroma components during the maintenance process are of great significance for improving the quality of cigars. Summary of the Invention
[0005] The purpose of this invention is to overcome the problems existing in the prior art and provide a method for evaluating the quality of cigar maintenance. This method establishes a three-dimensional comprehensive evaluation method to evaluate the change pattern and quality of aroma components in cigars during maintenance, providing a scientific basis for precise cigar maintenance.
[0006] To address the aforementioned technical problems, this invention provides a method for evaluating the quality of cigar preservation, the method comprising the following steps: S1. Collect the mainstream particulate matter and gaseous components of the sample to be tested, and extract the extract; S2. Perform GC / MS analysis on the extract to quantitatively analyze the content and proportion of aroma components in the extract, and obtain the content of alkaline aroma components (A content), neutral aroma components (B content), acidic aroma components (C content), total aroma components (D content), proportion of alkaline aroma components to total aroma components (E proportion), proportion of neutral aroma components to total aroma components (F proportion), and proportion of acidic aroma components to total aroma components (G proportion). S3. Based on the data obtained in step S2, the sensory total score is obtained through data processing and analysis. The overall quality of the cigar after maintenance is determined based on the sensory total score. Wherein, the total sensory score = 0.135 × A content + 0.160 × B content + 0.157 × C content + 0.164 × D content - 0.133 × E ratio + 0.134 × G ratio.
[0007] Preferably, in step S1, the process of capturing the particulate matter and gaseous components of the mainstream flue gas of the sample to be tested includes: using a linear smoke extractor to draw the sample to be tested, capturing the particulate matter of the mainstream flue gas through a filter, and capturing the gaseous components of the mainstream flue gas through an adsorption tube.
[0008] Preferably, the filter is a Cambridge filter and the adsorption tube is a CX-572 adsorption tube.
[0009] Preferably, in step S1, the extraction solvent used for extraction is carbon disulfide and / or methanol, and more preferably carbon disulfide and methanol.
[0010] Preferably, the extraction conditions include at least the following: a rotation speed of 100-150 rpm and a time of 20-40 min.
[0011] Preferably, in step S2, the step of quantitatively analyzing the content and proportion of aroma components in the extract includes quantitative analysis of the target analyte using the internal standard method.
[0012] Preferably, in step S2, the extract is derivatized before quantitative analysis of the content (C content) of the acidic aroma components; Preferably, the derivatization process includes mixing the extract, N,O-bis(trimethylsilyl)trifluoroacetamide, and internal standard solution.
[0013] Preferably, the internal standard solution is an octanoic acid solution, and the concentration of octanoic acid in the octanoic acid solution is 0.8-1 mg / mL.
[0014] Preferably, the mixing conditions include at least the following: a temperature of 70-80°C and a time of 1-2 hours.
[0015] Preferably, the chromatographic column used for GC / MS analysis is a DM-5MS capillary column.
[0016] Preferably, the initial temperature of the GC / MS program is not lower than 40°C, held for 0.5-3 min, and then increased to 280-300°C at a rate of 2-5°C / min, held for at least 15 min.
[0017] Preferably, the sample to be tested is selected from at least one of the following cigar types: Crown, Robusto, Bull, Pyramid, and Broad Hill.
[0018] The beneficial effects of the present invention through the above technical solution are as follows: The method for evaluating the maintenance quality of cigars provided by this invention involves GC / MS analysis of an extract containing mainstream particulate matter and gaseous components of the smoke to obtain the contents of alkaline aroma components (A content), neutral aroma components (B content), acidic aroma components (C content), total aroma components (D content), the proportion of alkaline aroma components to the total aroma components (E proportion), the proportion of neutral aroma components to the total aroma components (F proportion), and the proportion of acidic aroma components to the total aroma components (G proportion). After data processing and analysis, a total sensory score is obtained, and a sensory evaluation fitting equation is established. The sensory quality of cigars can be predicted through aroma component data, providing a scientific basis for the precise maintenance of cigars. Detailed Implementation
[0019] The endpoints and any values of the ranges disclosed herein are not limited to the precise ranges or values, and these ranges or values should be understood to include values close to these ranges or values. For numerical ranges, the endpoint values of the various ranges, the endpoint values of the various ranges and individual point values, and individual point values can be combined with each other to obtain one or more new numerical ranges, which should be considered as specifically disclosed herein.
[0020] As mentioned above, the present invention provides a method for evaluating the maintenance quality of cigars, the method comprising the following steps: S1. Collect the mainstream particulate matter and gaseous components of the sample to be tested, and extract the extract; S2. Perform GC / MS analysis on the extract to quantitatively analyze the content and proportion of aroma components in the extract, and obtain the content of alkaline aroma components (A content), neutral aroma components (B content), acidic aroma components (C content), total aroma components (D content), proportion of alkaline aroma components to total aroma components (E proportion), proportion of neutral aroma components to total aroma components (F proportion), and proportion of acidic aroma components to total aroma components (G proportion). S3. Based on the data obtained in step S2, the total sensory score is obtained through data processing and analysis. Wherein, the total sensory score = 0.135 × A content + 0.160 × B content + 0.157 × C content + 0.164 × D content - 0.133 × E ratio + 0.134 × G ratio.
[0021] During their research, the inventors of this invention discovered a method for evaluating the quality of cigar maintenance. This method involves GC / MS analysis of an extract containing mainstream particulate matter and gaseous components of cigar smoke to obtain the content of alkaline aroma components (A content), neutral aroma components (B content), acidic aroma components (C content), total aroma components (D content), the proportion of alkaline aroma components to the total aroma components (E proportion), the proportion of neutral aroma components to the total aroma components (F proportion), and the proportion of acidic aroma components to the total aroma components (G proportion). Furthermore, a sensory evaluation fitting equation is established, which can predict the sensory quality of cigars based on aroma component data, providing a scientific basis for precise cigar maintenance.
[0022] According to the present invention, preferably, in step S1, the process of capturing particulate matter and gaseous components of the mainstream flue gas of the sample to be tested includes: drawing the sample to be tested using a linear smoke extractor, capturing particulate matter of the mainstream flue gas through a filter, and capturing gaseous components of the mainstream flue gas through an adsorption tube. The inventors have found that, under this preferred embodiment, the capture efficiency of particulate matter and gaseous components in the mainstream flue gas can be improved.
[0023] According to the present invention, in order to further improve the collection efficiency of particulate matter and gaseous components in mainstream flue gas, preferably, the filter is a Cambridge filter and the adsorption tube is a CX-572 adsorption tube. There is no particular limitation on the diameter of the Cambridge filter; preferably, the Cambridge filter can be a Cambridge filter with a diameter of 50-60 mm. The CX-572 adsorption tube can be a CX-572 adsorption tube conventionally selected in the art; preferably, the CX-572 tube is manually prepared using a Sigma-Aldrich® SPE column filled with 300 mg Carboxen 572 (20 / 45 mesh size).
[0024] According to the present invention, in order to further improve the extraction efficiency of the effective components in the extract, preferably, in step S1, the extraction solvent used for extraction is carbon disulfide and / or methanol, preferably carbon disulfide and methanol.
[0025] In this invention, when the extraction solvent is carbon disulfide and methanol, preferably, the volume ratio of carbon disulfide to methanol is 1:3-5, which can be 1:3, 1:4, 1:5, or any value between the two aforementioned values.
[0026] According to the present invention, in order to further improve the extraction efficiency of the active ingredients in the extract, preferably, the extraction conditions include at least the following: a rotation speed of 100-150 rpm, which can be 100 rpm, 120 rpm, 140 rpm, 150 rpm, or any value between the two aforementioned values; and a time of 20-40 min, which can be 20 min, 30 min, 40 min, or any value between the two aforementioned values.
[0027] In this invention, the method further includes: in step S2, filtering the extract before performing GC / MS analysis. The inventors have found that, under this preferred embodiment, the influence of impurities in the extract on the results can be reduced, thereby improving the accuracy of the GC / MS analysis.
[0028] According to the present invention, preferably, step S2, the step of quantitatively analyzing the content and proportion of aroma components in the extract includes quantitative analysis of the target analyte using the internal standard method.
[0029] According to the present invention, preferably, in step S2, the extract is subjected to derivatization treatment before quantitative analysis of the content (C content) of the acidic aroma components. The inventors have found that, under this preferred embodiment, the extraction efficiency of the acidic aroma components in the extract can be further improved.
[0030] According to the present invention, in order to further improve the extraction efficiency of acidic aroma components in the extract, preferably, the derivatization process includes: mixing the extract, N,O-bis(trimethylsilyl)trifluoroacetamide and internal standard solution.
[0031] According to the present invention, in order to further improve the extraction efficiency of acidic aroma components in the extract, preferably, the internal standard solution is an octanoic acid solution, wherein the concentration of octanoic acid in the octanoic acid solution is 0.8-1 mg / mL, which can be 0.8 mg / mL, 0.9 mg / mL, or 1 mg / mL.
[0032] According to the present invention, preferably, the mixing conditions include at least: a temperature of 70-80°C, which can be 70°C, 75°C, 80°C, or any value between the two aforementioned values; and a time of 1-2 hours, which can be 1 hour, 1.5 hours, 2 hours, or any value between the two aforementioned values.
[0033] According to the present invention, in order to further improve the accuracy of GC / MS quantitative analysis, preferably, the chromatographic column used for GC / MS analysis is a DM-5MS capillary column.
[0034] According to the present invention, in order to further improve the accuracy of GC / MS quantitative analysis, preferably, the initial temperature of the GC / MS temperature program is not lower than 40°C, held for 0.5-3 min, and then increased to 280-300°C at 2-5°C / min, and held for at least 15 min.
[0035] In this invention, the initial temperature for the programmed temperature rise of the GC / MS is 40-60°C, which can be 40°C, 50°C, 60°C, or any value between the two aforementioned values. For example, when detecting the content of alkaline aroma components, the initial temperature is 60°C; when detecting the content of neutral aroma components, the initial temperature is 60°C; and when detecting acidic aroma components, the initial temperature is 40°C.
[0036] According to the present invention, preferably, the sample to be tested is selected from at least one of the following: Crown, Robusto, Bull, Pyramid, and Broad Hill cigars.
[0037] In this invention, the method can be applied to cigars with different curing times, and the curing time of the cigars used as test samples can be 0-300 days.
[0038] According to a particularly preferred embodiment of the present invention, a method for evaluating the maintenance quality of cigars is provided, the method comprising the following steps: S1. The sample to be tested is drawn using a linear smoking machine. The particulate matter of the mainstream smoke is captured through a filter, and the gaseous components of the mainstream smoke are captured through an adsorption tube. Extraction is performed for 20-40 minutes at a speed of 100-150 rpm to obtain the extract. The filter is a Cambridge filter, and the adsorption tube is a CX-572 adsorption tube. The extraction solvent used is carbon disulfide and methanol in a volume ratio of 1:3-5. The sample to be tested is selected from at least one of the following cigar types: Crown, Robusto, Bull, Pyramid, and Wide Hill. S2. The extract was analyzed by GC / MS. The content and proportion of aroma components in the extract were quantitatively analyzed by internal standard method to obtain the content of alkaline aroma components (A content), neutral aroma components (B content), acidic aroma components (C content), total aroma components (D content), proportion of alkaline aroma components (E content), proportion of neutral aroma components (F content), and proportion of acidic aroma components (G content). Before quantitative analysis of the content of acidic aroma components (C content), the extract was derivatized. The derivatization process included reacting the extract, N,O-bis(trimethylsilyl)trifluoroacetamide and 0.8-1 mg / mL octanoic acid solution at a temperature of 70-80℃ for 1-2 h. The GC / MS analysis used a DM-5MS capillary column; the initial temperature of the GC / MS program should not be lower than 40℃, held for 0.5-3 min, and then increased to 280-300℃ at 2-5℃ / min, held for at least 15 min. S3. Based on the data obtained in step S2, the sensory total score is obtained through data processing and analysis. The overall quality of the cigar after maintenance is determined based on the sensory total score. Wherein, the total sensory score = 0.135 × A content + 0.160 × B content + 0.157 × C content + 0.164 × D content - 0.133 × E ratio + 0.134 × G ratio.
[0039] The present invention will be described in detail below through embodiments.
[0040] The instruments used in the following embodiments include: a linear cigar smoking machine (SML 1000C, Hefei Institutes of Physical Science, Chinese Academy of Sciences); a constant temperature water bath (SHA-C, Ronghua Instrument Manufacturing Co., Ltd., Jintan City); a vortex oscillator (MultiReax, Heidolph GmbH, Germany); a CP224S electronic analytical balance (sensitivity 0.0001g, Sartorius GmbH, Germany); an Agilent 7890-5977C GC-MS system (Agilent Technologies, USA); a 3-30KS high-speed refrigerated centrifuge (Sigma GmbH, Germany); and a Milli-Q ultrapure water system (Millipore, USA).
[0041] In the following examples, phenethyl acetate (99%, Henan Songjian Standard Technology Co., Ltd.); aroma component mixed standard solution (Henan Songjian Standard Technology Co., Ltd.); dichloromethane and acetonitrile (chromatographic grade, TEDIA, USA); N,O-bis(trimethylsilyl)trifluoroacetamide, Sigma, USA; unless otherwise specified, all other raw materials were commercially available.
[0042] Example (1) Smoking of the tested cigar samples Use a linear cigar smoking machine to smoke cigars, one cigar per group. Use a 55mm diameter Cambridge filter to capture particulate matter in the mainstream cigarette smoke and a 300mg adsorption tube to capture gaseous components in the mainstream cigarette smoke. (2) Preparation of the sample solution to be tested Cambridge filters and CX-572 particles were transferred from the column to a glass vial, and 2 mL of carbon disulfide was added to the vial. 8 mL of methanol was then slowly added, the vial was sealed, and the mixture was gently shaken at 120 cycles per minute for 30 minutes on a rotary shaker. The sample was allowed to stand for at least 1 minute. Finally, 1 mL of the eluent was transferred to a 1.5 mL autosampler vial and analyzed by GC / MS. The acidic component silanization derivatization was performed as follows: 1 ml of the particulate matter and gaseous extract filtered through a 0.45 μm microporous membrane was transferred into a chromatographic bottle, 100 μL of N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was added, along with 100 μL of internal standard solution (octanoic acid solution). The bottle was sealed and derivatized in a 75°C water bath for 1 h. After cooling to room temperature, the solution was analyzed by GC-MS. (3) The chromatographic conditions used include: Alkaline aroma components: The capillary column used was a DB-5MS capillary column [60m (length) × 0.25mm (inner diameter) × 1.0μm (film thickness)], the carrier gas was helium, and the carrier gas flow rate was 1.9mL / min; the injection port temperature was 280℃, and the split ratio was 10:1; the temperature program was as follows: initial temperature 60℃, hold for 2 min; increase to 250℃ at 2℃ / min, hold for 0 min; increase to 290℃ at 5℃ / min, hold for 15 min; MS conditions: electron impact ionization (EI) source, full scan and SIM scan mode, electron energy 70eV; Neutral aroma components: The capillary column used was a DB-5MS capillary column [60m (length) × 0.25mm (inner diameter) × 1.0μm (film thickness)], the carrier gas was helium, and the carrier gas flow rate was 1.0mL / min; the injection port temperature was 280℃, and the split ratio was 10:1; the temperature program was: initial temperature 60℃, hold for 0.5min; increase to 300℃ at 5℃ / min, hold for 15min; MS conditions: electron impact ionization (EI) source, full scan and SIM scan mode, electron energy 70eV; Acidic aroma components: A DB-5MS capillary column [60m (length) × 0.25mm (inner diameter) × 1.0μm (film thickness)] was used as the capillary column, with helium as the carrier gas at a flow rate of 1.5mL / min; the injection port temperature was 280℃, and the split ratio was 10:1; the temperature program was as follows: initial temperature 40℃, hold for 3 min; increase to 280℃ at 4℃ / min, hold for 20 min; MS conditions: electron impact ionization (EI) source, full scan and SIM scan, electron energy 70eV. Qualitative analysis was performed using the retention times and selected ions of the chromatographic peaks of compounds in the reference standard and sample, and quantification was performed using the internal standard method for peak area.
[0043] (4) Examples of detection of aroma components in cigar smoke at different curing times The cigar samples processed according to the above steps were analyzed; qualitative analysis was performed using the retention time of the control standard and the target analyte, and the content of the target analyte was quantified using the internal standard method.
[0044] This method was used to test samples at 10 different curing stages with curing times of 0d, 28d, 56d, 84d, 112d, 140d, 168d, 196d, 224d, 252d and 280d. The results after data processing are shown in Table 1.
[0045] Table 1. Content and proportion of aroma components in cigar smoke at different curing times
[0046] (5) Five cigar smoking experts were invited to evaluate cigars that had been maintained for different periods. The average score of the five experts was taken for each indicator. The sensory evaluation results are shown in Table 2.
[0047] Table 2 Sensory evaluation results of cigars at different curing times
[0048] (6) Correlation analysis was performed on the aroma component data and sensory evaluation data. The results are shown in Table 3.
[0049] Table 3. Correlation between aroma components and sensory evaluation results
[0050] The results in Tables 2 and 3 show that the indicators that are correlated with the total sensory evaluation score are: content of alkaline aroma components (A content), content of neutral aroma components (B content), content of acidic aroma components (C content), total aroma components (D content), proportion of alkaline aroma components to total aroma components (E proportion), and proportion of acidic aroma components to total aroma components (G proportion).
[0051] (7) PLS regression analysis was performed on the aroma component data and the total sensory score, and one principal component was extracted to obtain the fitting equation; Sensory total score = 0.135 × A content + 0.160 × B content + 0.157 × C content + 0.164 × D content - 0.133 × E proportion + 0.134 × G proportion; where the fitted equation R 2 =0.695.
[0052] Comparative Example The volatile components in cigar tobacco were analyzed using gas chromatography-mass spectrometry (GC-MS) (7890A / 5975C, Agilent Technologies, Inc., Santa Clara, CA, USA). Sample pretreatment was as follows: 2.0 g of cigar tobacco powder was accurately weighed and placed in a 50 mL centrifuge tube. 10 mL of water was added and the mixture was soaked for 10 min. Then, 10 mL of acetonitrile and 0.1 mL of internal standard solution (phenethyl acetate, 100 µg / mL) were added, vortexed for 1 min, and then frozen for 10 min. Subsequently, a high-performance sample extraction kit was added, and the mixture was vortexed for 2 min to prevent magnesium sulfate from clumping. After centrifugation at 4000 rpm for 10 min, 1 mL of the supernatant was transferred to a 1.5 mL centrifuge tube. A high-performance pigment sample purification kit was added, and the mixture was vortexed at 2000 rpm for 2 min, then centrifuged at 6000 rpm for 2 min. The supernatant was collected for GC-MS analysis.
[0053] The GC-MS analysis conditions were as follows: a DB-5 quartz capillary column (60m × 0.25mm × 0.25µm), an injection port temperature of 270℃, a split ratio of 20:1, and an injection volume of 1µL; helium was used as the carrier gas, the flow rate was constant at 1mL / min, and the solvent delay time was 6min. The temperature program was as follows: an initial temperature of 60℃ held for 0.5min, then increased to 280℃ at a rate of 2℃ / min and held for 30min. The ion source was an electron impact (EI) source with an ionization voltage of 70eV, an ion source temperature of 230℃, a transfer line temperature of 280℃, and a mass scan range of 30-350 amu.
[0054] This method was used to test samples at 10 different curing stages with curing times of 0d, 28d, 56d, 84d, 112d, 140d, 168d, 196d, 224d, 252d, and 280d. The results after data processing are shown in Table 4. Correlation analysis was performed on the aroma component data and sensory evaluation data, and the results are shown in Table 5.
[0055] Table 4. Content and proportion of aroma components in cigar smoke at different curing times
[0056] Table 5. Correlation between aroma components and sensory evaluation results
[0057] As shown in Tables 4 and 5, compared with the examples, the correlation analysis results of the comparative examples show a decrease in the number of indicators associated with the total sensory score. After PLS analysis, the fitting equation R... 2 =0.642, which is less illustrative than the example.
[0058] The preferred embodiments of the present invention have been described in detail above; however, the present invention is not limited thereto. Within the scope of the inventive concept, various simple modifications can be made to the technical solutions of the present invention, including combinations of various technical features in any other suitable manner. These simple modifications and combinations should also be considered as the content disclosed in the present invention and are all within the protection scope of the present invention.
Claims
1. A method of evaluating the quality of cigar maintenance, characterized in that, The method comprises the following steps: S1, trapping the particulate matter and gas phase components of the mainstream smoke of the sample to be tested, and extracting to obtain an extract; S2, performing GC / MS analysis on the extract, quantitatively analyzing the content and proportion of the flavor components in the extract, and obtaining the content of basic flavor components (A content), the content of neutral flavor components (B content), the content of acidic flavor components (C content), the total amount of flavor components (D content), the proportion of basic flavor components in the total amount of flavor components (E proportion), the proportion of neutral flavor components in the total amount of flavor components (F proportion), and the proportion of acidic flavor components in the total amount of flavor components (G proportion); S3, obtaining a total sensory score by data processing and analysis based on the data obtained in step S2, and determining the comprehensive quality of the maintained cigar according to the total sensory score; The total sensory score = 0.135 × A content + 0.160 × B content + 0.157 × C content + 0.164 × D content - 0.133 × E proportion + 0.134 × G proportion.
2. The method of claim 1, wherein, In step S1, the process of trapping the particulate matter and gas phase components of the mainstream smoke of the sample to be tested comprises: using a linear smoking machine to smoke the sample to be tested, trapping the particulate matter of the mainstream smoke by a filter, and trapping the gas phase components of the mainstream smoke by an adsorption tube.
3. The method of claim 2, wherein, The filter is a Cambridge filter, and the adsorption tube is a CX-572 adsorption tube.
4. The method of claim 1, wherein, In step S1, the extraction solvent used for extraction is carbon disulfide and / or methanol, preferably carbon disulfide and methanol. Preferably, the extraction conditions at least include: rotation speed of 100-150 rpm, and time of 20-40 min.
5. The method according to any one of claims 1 to 4, characterized in that, In step S2, the step of quantitatively analyzing the content and proportion of the flavor components in the extract comprises quantitative analysis of the target by an internal standard method.
6. The method of claim 5, wherein, In step S2, before quantitatively analyzing the content of acidic flavor components (C content), the extract is subjected to a derivatization treatment.
7. The method of claim 6, wherein, The process of the derivatization treatment comprises: mixing the extract, N, O-bis (trimethylsilyl) trifluoroacetamide, and an internal standard solution.
8. The method of claim 7, wherein, The internal standard solution is a n-octanoic acid solution, and the concentration of n-octanoic acid in the n-octanoic acid solution is 0.8-1 mg / mL; Preferably, the mixing conditions at least include: temperature of 70-80℃, and time of 1-2 h.
9. The method according to any one of claims 1 to 4, characterized in that, The chromatographic column used for GC / MS analysis is a DM-5MS capillary chromatographic column; Preferably, the initial temperature of the programmed temperature of the GC / MS is not less than 40℃, and is maintained for 0.5-3 min, then increased to 280-300℃ at a rate of 2-5℃ / min, and maintained for at least 15 min.
10. The method according to any one of claims 1 to 4, characterized in that, The sample to be tested is selected from at least one of the following types of cigar: crown type, Robusto type, Toro type, Pyramid type, and Belicoso type.