Seed dressing agent and preparation method thereof

Abstract

The invention discloses a seed dressing agent and a preparation method thereof. Comprising 30-50 parts of a chaetomium globosum QM bacterial agent, 30-50 parts of an enterobacter chinense 314 A1 bacterial agent, 20-40 parts of chitosan, 5-10 parts of a film-forming agent and 3-8 parts of a wetting dispersant. According to the seed dressing agent disclosed by the invention, the chaetomium globosum QM bacterial agent and the enterobacter chinense 314 A1 bacterial agent are added, so that the seedling rate and the growth condition of tomato seedlings are improved, and a synergistic interaction effect is achieved by combined use of the bacterial agent and the enterobacter chinense 314 A1 bacterial agent. By using the polylactic acid-polyethylene glycol copolymer and locust bean gum composite film-forming agent and the nekal and polyethylene glycol monohexadecyl ether composite wetting dispersant, the germination rate of the tomato seeds is increased, and synergistic interaction is achieved.

Description

Technical Field

[0001] This invention belongs to the field of agricultural biotechnology, specifically relating to a seed dressing agent suitable for tomato seeds and its preparation method. Background Technology

[0002] Seed dressing agents are pesticide formulations (including chemical and biological pesticides) used to treat crop seeds. Through a specific process, the agent is evenly coated on the seed surface, forming a protective film, which then exerts its effect after sowing. The main functions of seed dressing agents are: 1. Control of soil-borne and seed-borne diseases. Soil-borne diseases include wheat sheath blight, take-all, and root rot; corn smut and stem rot. These pathogens exist in the soil and first infect young roots and shoots after seed germination. Seed-borne diseases include rice bakanae disease and rice blast; and certain soybean diseases. Seed dressing agents form a localized "protective ring" around the seed, effectively killing or inhibiting pathogens on the soil and seed. 2. Control of underground and seedling pests. Underground pests include grubs, wireworms, cutworms, and mole crickets, which directly bite and feed on seeds, young roots, and underground parts of seedlings, causing gaps in the rows. Systemic insecticides are absorbed by the seeds and translocated to the seedlings; pests that feed on them die from poisoning. 3. Enhances stress resistance and promotes growth: Some seed treatment agents contain plant growth regulators or trace elements, which can stimulate seed germination, promote root development, make seedlings stronger, and improve their resistance to drought, cold, and salinity. 4. Increases yield and improves quality: By ensuring full, uniform, and robust seedlings, a solid foundation is laid for high crop yields. Healthy roots and plants can absorb nutrients and water more efficiently, ultimately leading to increased yield and improved quality.

[0003] Tomato seeds have a low germination rate and poor activity due to uneven maturity and difficulty in visual inspection and selection. Germination agents are often used in agricultural seedling cultivation, but tomato seedlings grown with these agents tend to be leggy, further reducing the seedling survival rate. Seed coating is one solution, but the active ingredients in current tomato seed coatings are generally chemical pesticide components, which have a long residual effect in the soil and pose environmental risks with long-term use. Summary of the Invention

[0004] The purpose of this invention is to provide a seed dressing agent suitable for tomato seeds and its preparation method.

[0005] A seed dressing agent, comprising Chaetomium globulus ( Chaetomium globosum 30-50 portions of QM bacterial agent, and West China Enterobacter ( Enterobacter huaxiensis 314 A1 bacterial agent 30-50 parts, chitosan 20-40 parts, film-forming agent 5-10 parts, wetting and dispersing agent 3-8 parts.

[0006] The Chaetomium globulus ( Chaetomium globosum The QM bacteria, with accession number CGMCC NO: 41324, was purchased from the China General Microbiological Culture Collection Center; the *Enterobacter xanthipes* (QM) mentioned herein... Enterobacter huaxiensis )314 A1, with accession number CCTCC NO: M20232090, was purchased from the China Center for Type Culture Collection.

[0007] The Chaetomium globulus ( Chaetomium globosum The preparation method of QM inoculant is as follows: Take 130-250 parts by weight of solid fermentation raw material, add 100-300 parts of water and soak for 18-24 hours, put it into edible fungus cultivation bags, cover with a breathable cover, and sterilize at 121℃ for 30-50 minutes; after cooling to 20-30℃, inoculate with QM spore suspension, and incubate at 20-30℃ for 4-10 days. After the bag is full of mycelium, open the cover to ventilate, incubate for another 4-10 days, and then dry. Use a high-speed pulverizer to pulverize the culture material for later use.

[0008] The preparation method of the solid fermentation raw material is as follows: take 30-50 parts of corn, 50-100 parts of corn stalks, 20-30 parts of wheat bran and 10-20 parts of soybean meal according to the weight ratio, crush them and pass them through a 20-40 mesh sieve, add 3-8 parts of superphosphate, and mix them evenly to make the raw material.

[0009] The West China Enterobacter ( Enterobacter huaxiensis The preparation method of 314 A1 bacterial agent is as follows: *Enterobacter xanthipes* (West China Enterobacter) is prepared by... Enterobacter huaxiensis After being expanded and cultured, 314A1 was inoculated into a fermentation medium. After culturing, the cells were obtained by centrifugation. The wet cells were mixed with an equal weight of corn starch, dried, and then pulverized through a 40-100 mesh sieve to obtain the final product.

[0010] The fermentation medium consisted of: 10 g / L tryptone, 9.5 g / L soluble starch, 5 g / L yeast extract, 8.5 g / L ammonium chloride, 10 g / L sodium chloride, and 5 g / L (NH4)2 SO4, with a pH of 7.0.

[0011] The film-forming agent is a mixture of polylactic acid-polyethylene glycol copolymer and locust bean gum in a mass ratio of 2:1.

[0012] The wetting and dispersing agent is a mixture of splitting powder and polyethylene glycol monohexadecyl ether at a mass ratio of 1:1.

[0013] The preparation method of the seed dressing agent is carried out according to the following steps: chitosan, film-forming agent, and wetting and dispersing agent are put into a mixer and mixed for 2-4 minutes, and then *Chaetomium globosum* ( Chaetomium globosum QM bacterial agent and West China Enterobacter ( Enterobacter huaxiensis )314 A1 bacterial agent is placed in a grinder and ground for 1-3 minutes. Finally, the substances are mixed together and mixed evenly.

[0014] The method of using the seed dressing agent is as follows: during the tomato sowing period, the seed dressing agent is diluted with water and applied to the tomato seeds, which are then dried in the shade before sowing; the mass ratio of the seed dressing agent, water and tomato seeds is 1:(5-15):(100-150).

[0015] The beneficial effects of this invention are as follows: The seed dressing agent of this invention, with the addition of Chaetomium globosum QM inoculum and Enterobacter schlegelii 314 A1 inoculum, improves the seedling establishment rate and growth status of tomato seedlings, and the combined use has a synergistic effect. The use of polylactic acid-polyethylene glycol copolymer and locust bean gum composite film-forming agent, and the use of dispersant powder and polyethylene glycol monohexadecyl ether composite wetting and dispersing agent, improve the germination rate of tomato seeds, and have a synergistic effect. Detailed Implementation

[0016] To facilitate understanding of the present invention, a more comprehensive description will be given below. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a thorough and complete understanding of the disclosure of the present invention.

[0017] The following examples describe Chaetomium globosum ( Chaetomium globosum The QM bacteria, with accession number CGMCC NO: 41324, was purchased from the China General Microbiological Culture Collection Center; the *Enterobacter xanthipes* (QM) mentioned herein... Enterobacter huaxiensis )314 A1, with accession number CCTCC NO: M20232090, was purchased from the China Center for Type Culture Collection.

[0018] The following examples are of Chaetomium globulus ( Chaetomium globosum The preparation method of QM inoculant is as follows: Take 170 parts by weight of solid fermentation raw material, add 200 parts by weight of water and soak for 20 hours, put it into edible fungus cultivation bags, cover with a breathable cover, and sterilize at 121℃ for 40 minutes; after cooling to 25℃, inoculate with QM spore suspension (adjust the bacterial content in the suspension to 5×10⁻⁶). 8 (CFU / mL, inoculum 1%), incubated at 25℃ for 8 days. After the bag is fully covered with mycelium, the lid is opened for ventilation, and the bag is incubated for another 8 days before being dried. The culture medium is then pulverized using a high-speed pulverizer for later use. The preparation method of the solid fermentation raw material is as follows: according to the weight proportions, take 40 parts of corn, 85 parts of corn stalks, 25 parts of wheat bran, and 15 parts of soybean meal, pulverize them, pass them through a 20-40 mesh sieve, add 5 parts of superphosphate, and mix evenly.

[0019] The West China Enterobacter ( Enterobacter huaxiensis The preparation method of 314 A1 bacterial agent is as follows: *Enterobacter xanthipes* (West China Enterobacter) is prepared by... Enterobacter huaxiensis After expansion culture (the bacterial content in the suspension was adjusted to 5 × 10⁻⁶ after expansion culture), 314A1 was cultured in a large-scale culture. 8 The inoculum (cfu / mL, 1% inoculum) was inoculated into the fermentation medium, and after cultivation, the cells were obtained by centrifugation. The wet cells were mixed with an equal weight of corn starch, dried, and then pulverized through a 60-mesh sieve to obtain the final product. The fermentation medium consisted of: 10 g / L tryptone, 9.5 g / L soluble starch, 5 g / L yeast extract, 8.5 g / L ammonium chloride, 10 g / L sodium chloride, and 5 g / L (NH4)2 SO4, pH 7.0.

[0020] Example 1 A seed dressing agent, comprising Chaetomium globulus ( Chaetomium globosum 40 portions of QM bacterial agent, West China Enterobacter ( Enterobacter huaxiensis )314 A1 bacterial agent 40 parts, chitosan 30 parts, film-forming agent 9 parts, wetting and dispersing agent 6 parts; the film-forming agent is a mixture of polylactic acid-polyethylene glycol copolymer and locust bean gum in a mass ratio of 2:1; the wetting and dispersing agent is a mixture of splitting powder and polyethylene glycol monohexadecyl ether in a mass ratio of 1:1.

[0021] The preparation method of the seed dressing agent is carried out according to the following steps: chitosan, film-forming agent, and wetting and dispersing agent are put into a mixer and mixed for 3 minutes, and then *Chaetomium globosum* ( Chaetomium globosum QM bacterial agent and West China Enterobacter ( Enterobacter huaxiensis )314 A1 bacterial agent is placed in a grinder and ground for 2 minutes. Finally, the substances are mixed together and mixed evenly.

[0022] Example 2 A seed dressing agent, comprising Chaetomium globulus ( Chaetomium globosum 30 portions of QM bacterial agent, West China Enterobacter ( Enterobacter huaxiensis )314 A1 bacterial agent 30 parts, chitosan 22 parts, film-forming agent 6 parts, wetting and dispersing agent 4 parts; the film-forming agent is a mixture of polylactic acid-polyethylene glycol copolymer and locust bean gum in a mass ratio of 2:1; the wetting and dispersing agent is a mixture of splitting powder and polyethylene glycol monohexadecyl ether in a mass ratio of 1:1.

[0023] The preparation method of the seed dressing agent is carried out according to the following steps: chitosan, film-forming agent, and wetting and dispersing agent are put into a mixer and mixed for 2 minutes, and then *Chaetomium globosum* ( Chaetomium globosum QM bacterial agent and West China Enterobacter ( Enterobacter huaxiensis )314 A1 bacterial agent is placed in a grinder and ground for 1 minute. Finally, the substances are mixed together and mixed evenly.

[0024] Example 3 A seed dressing agent, comprising Chaetomium globulus ( Chaetomium globosum 50 portions of QM bacterial agent, West China Enterobacter ( Enterobacter huaxiensis )314 A1 bacterial agent 50 parts, chitosan 40 parts, film-forming agent 9 parts, wetting and dispersing agent 8 parts; the film-forming agent is a mixture of polylactic acid-polyethylene glycol copolymer and locust bean gum in a mass ratio of 2:1; the wetting and dispersing agent is a mixture of splitting powder and polyethylene glycol monohexadecyl ether in a mass ratio of 1:1.

[0025] The preparation method of the seed dressing agent is carried out according to the following steps: chitosan, film-forming agent, and wetting and dispersing agent are put into a mixer and mixed for 4 minutes, and then *Chaetomium globosum* ( Chaetomium globosum QM bacterial agent and West China Enterobacter ( Enterobacter huaxiensis )314 A1 bacterial agent is placed in a grinder and ground for 3 minutes. Finally, the substances are mixed together and mixed evenly.

[0026] Comparative Example 1 A seed dressing agent, comprising Chaetomium globulus ( Chaetomium globosum The mixture contains 80 parts of QM bacterial agent, 30 parts of chitosan, 9 parts of film-forming agent, and 6 parts of wetting and dispersing agent. The film-forming agent is a mixture of polylactic acid-polyethylene glycol copolymer and locust bean gum in a mass ratio of 2:1. The wetting and dispersing agent is a mixture of splitting powder and polyethylene glycol monohexadecyl ether in a mass ratio of 1:1.

[0027] The preparation method of the seed dressing agent is carried out according to the following steps: chitosan, film-forming agent, and wetting and dispersing agent are put into a mixer and mixed for 3 minutes, and then *Chaetomium globosum* ( Chaetomium globosum QM bacterial agent is placed in a grinder and ground for 2 minutes. Finally, the substances are mixed together and thoroughly mixed.

[0028] Comparative Example 2 A seed dressing agent, comprising West China Enterobacter ( Enterobacter huaxiensis )314 A1 bacterial agent 80 parts, chitosan 30 parts, film-forming agent 9 parts, wetting and dispersing agent 6 parts; the film-forming agent is a mixture of polylactic acid-polyethylene glycol copolymer and locust bean gum in a mass ratio of 2:1; the wetting and dispersing agent is a mixture of splitting powder and polyethylene glycol monohexadecyl ether in a mass ratio of 1:1.

[0029] The preparation method of the seed dressing agent is carried out according to the following steps: chitosan, film-forming agent, and wetting and dispersing agent are put into a mixer and mixed for 3 minutes, and then *Enterobacter xanthipes* (West China Enterobacter) is added. Enterobacter huaxiensis )314 A1 bacterial agent is placed in a grinder and ground for 2 minutes. Finally, the substances are mixed together and mixed evenly.

[0030] Comparative Example 3 A seed dressing agent, comprising Chaetomium globulus ( Chaetomium globosum 40 portions of QM bacterial agent, West China Enterobacter ( Enterobacter huaxiensis )314 A1 bacterial agent 40 parts, chitosan 30 parts, polylactic acid-polyethylene glycol copolymer 9 parts, wetting and dispersing agent 6 parts; the wetting and dispersing agent is a mixture of splitting powder and polyethylene glycol monohexadecyl ether in a mass ratio of 1:1.

[0031] The preparation method of the seed dressing agent is carried out according to the following steps: chitosan, polylactic acid-polyethylene glycol copolymer, and wetting and dispersing agent are put into a mixer and mixed for 3 minutes, and then *Chaetomium globulus* ( Chaetomium globosum QM bacterial agent and West China Enterobacter ( Enterobacter huaxiensis )314 A1 bacterial agent is placed in a grinder and ground for 2 minutes. Finally, the substances are mixed together and mixed evenly.

[0032] Comparative Example 4 A seed dressing agent, comprising Chaetomium globulus ( Chaetomium globosum 40 portions of QM bacterial agent, West China Enterobacter ( Enterobacter huaxiensis )314 A1 bacterial agent 40 parts, chitosan 30 parts, locust bean gum 9 parts, wetting and dispersing agent 6 parts; the wetting and dispersing agent is a mixture of splitting powder and polyethylene glycol monohexadecyl ether in a mass ratio of 1:1.

[0033] The preparation method of the seed dressing agent is carried out according to the following steps: chitosan, locust bean gum, and wetting and dispersing agent are put into a mixer and mixed for 3 minutes, and then *Chaetomium globosum* ( Chaetomium globosum QM bacterial agent and West China Enterobacter ( Enterobacter huaxiensis )314 A1 bacterial agent is placed in a grinder and ground for 2 minutes. Finally, the substances are mixed together and mixed evenly.

[0034] Comparative Example 5 A seed dressing agent, comprising Chaetomium globulus ( Chaetomium globosum 40 portions of QM bacterial agent, West China Enterobacter ( Enterobacter huaxiensis )314 A1 bacterial agent 40 parts, chitosan 30 parts, film-forming agent 9 parts, and stretching powder 6 parts; the film-forming agent is a mixture of polylactic acid-polyethylene glycol copolymer and locust bean gum in a mass ratio of 2:1.

[0035] The preparation method of the seed dressing agent is carried out according to the following steps: chitosan, film-forming agent, and splitting powder are put into a mixer and mixed for 3 minutes, and then *Chaetomium globosum* ( Chaetomium globosum QM bacterial agent and West China Enterobacter ( Enterobacter huaxiensis )314 A1 bacterial agent is placed in a grinder and ground for 2 minutes. Finally, the substances are mixed together and mixed evenly.

[0036] Comparative Example 6 A seed dressing agent, comprising Chaetomium globulus ( Chaetomium globosum 40 portions of QM bacterial agent, West China Enterobacter ( Enterobacter huaxiensis )314 A1 bacterial agent 40 parts, chitosan 30 parts, film-forming agent 9 parts, polyethylene glycol monohexadecyl ether 6 parts; the film-forming agent is a mixture of polylactic acid-polyethylene glycol copolymer and locust bean gum in a mass ratio of 2:1.

[0037] The preparation method of the seed dressing agent is carried out according to the following steps: chitosan, film-forming agent, and polyethylene glycol monohexadecyl ether are put into a mixer and mixed for 3 minutes, and then *Chaetoceros globulus* ( Chaetomium globosum QM bacterial agent and West China Enterobacter ( Enterobacter huaxiensis )314 A1 bacterial agent is placed in a grinder and ground for 2 minutes. Finally, the substances are mixed together and mixed evenly.

[0038] Experimental example: Tomato seeds were treated with the seed dressing agents prepared in Examples 1-3 and Comparative Examples 1-6, respectively, with a mass ratio of seed dressing agent, water, and tomato seeds of 1:8:120. Each treatment contained 100 seeds, replicated three times. Germination rate was assessed after 7 days. Seven days after emergence, tomato seedlings were placed in a light-controlled incubator with a low-temperature environment (22°C during the day and 12°C at night) for 48 hours. After 48 hours, they were removed and allowed to continue growing indoors at 25°C during the day and 16°C at night. Seedling establishment rate was assessed when the seedlings had two true leaves. The seedlings were cultured for another 30 days, and plant height, fresh weight, and root length were measured.

[0039] The experimental results were statistically analyzed using SPSS 24.0 software. Quantitative data were expressed as mean ± standard deviation (x ± σ). The Kolmogorov-Smirnov test was used to test the normality of the data. For normally distributed data, the t-test was used to compare the differences in means between two groups, with P < 0.05 considered statistically significant. The measurement results are shown in Tables 1-3. Table 1

[0040] Note: * indicates that compared with Example 1 group, P<0.05.

[0041] Table 2

[0042] Note: * indicates that compared with Example 1 group, P<0.05.

[0043] Table 3

[0044] Note: * indicates that compared with Example 1 group, P<0.05.

[0045] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A seed dressing agent, characterized in that, Including Chaetomium coccidioides ( Chaetomium globosum 30-50 portions of QM bacterial agent, and West China Enterobacter ( Enterobacter huaxiensis 314 A1 bacterial agent 30-50 parts, chitosan 20-40 parts, film-forming agent 5-10 parts, wetting and dispersing agent 3-8 parts.

2. The seed dressing agent according to claim 1, characterized in that, The Chaetomium globulus ( Chaetomium globosum The QM bacteria preservation number is CGMCC NO: 41324, and the *Enterobacter xanthipes* described is... Enterobacter huaxiensis )314 A1 has the accession number CCTCC NO: M20232090.

3. The seed dressing agent according to claim 1, characterized in that, The Chaetomium globulus ( Chaetomium globosum The preparation method of QM inoculant is as follows: Take 130-250 parts by weight of solid fermentation raw material, add 100-300 parts of water and soak for 18-24 hours, put it into edible fungus cultivation bags, cover with a breathable cover, and sterilize at 121℃ for 30-50 minutes; after cooling to 20-30℃, inoculate with QM spore suspension, and incubate at 20-30℃ for 4-10 days. After the bag is full of mycelium, open the cover to ventilate, incubate for another 4-10 days, and then dry. Use a high-speed pulverizer to pulverize the culture material for later use.

4. The seed dressing agent according to claim 3, characterized in that, The preparation method of the solid fermentation raw material is as follows: take 30-50 parts of corn, 50-100 parts of corn stalks, 20-30 parts of wheat bran and 10-20 parts of soybean meal according to the weight ratio, crush them and pass them through a 20-40 mesh sieve, add 3-8 parts of superphosphate, and mix them evenly to make the raw material.

5. The seed dressing agent according to claim 1, characterized in that, The West China Enterobacter ( Enterobacter huaxiensis The preparation method of 314 A1 bacterial agent is as follows: *Enterobacter xanthipes* (West China Enterobacter) is prepared by... Enterobacter huaxiensis After being expanded and cultured, 314A1 was inoculated into a fermentation medium. After culturing, the cells were obtained by centrifugation. The wet cells were mixed with an equal weight of corn starch, dried, and then pulverized through a 40-100 mesh sieve to obtain the final product.

6. The seed dressing agent according to claim 5, characterized in that, The fermentation medium consisted of: 10 g / L tryptone, 9.5 g / L soluble starch, 5 g / L yeast extract, 8.5 g / L ammonium chloride, 10 g / L sodium chloride, and 5 g / L (NH4)2 SO4, with a pH of 7.

0.

7. The seed dressing agent according to claim 1, characterized in that, The film-forming agent is a mixture of polylactic acid-polyethylene glycol copolymer and locust bean gum in a mass ratio of 2:

1.

8. The seed dressing agent according to claim 1, characterized in that, The wetting and dispersing agent is a mixture of splitting powder and polyethylene glycol monohexadecyl ether at a mass ratio of 1:

1.

9. The method for preparing the seed dressing agent according to claim 1, characterized in that, Follow these steps: Place chitosan, film-forming agent, and wetting and dispersing agent into a mixer and mix for 2-4 minutes. Then add *Chaetomium globosum* (…). Chaetomium globosum QM bacterial agent and West China Enterobacter ( Enterobacter huaxiensis )314 A1 bacterial agent is placed in a grinder and ground for 1-3 minutes. Finally, the substances are mixed together and mixed evenly.

10. The method of using the seed dressing agent according to claim 1, characterized in that, During the tomato sowing period, the seed dressing agent is diluted with water and applied to the tomato seeds, then dried in the shade before sowing; the mass ratio of the seed dressing agent, water and tomato seeds is 1:(5-15):(100-150).

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