Scald repair cream taking sorghum wax as effective component and preparation method of scald repair cream

Abstract

The invention relates to a scald repair cream taking sorghum wax as an effective component and a preparation method thereof, the scald repair cream is composed of sorghum wax, goosegrass extract, glycerin, ethanol and vitamin C. The effective component sorghum wax has a good treatment effect on skin scald, and the prepared scald repair cream can promote wound healing, reduce swelling and pain, and improve the healing effect of the skin. The preparation can promote epidermis recovery and reduce scars, and is a green, safe and non-irritant scald repair preparation.

Description

Technical Field

[0001] This invention belongs to the field of burn prevention and treatment technology, specifically relating to a burn repair ointment with sorghum wax as the active ingredient and its preparation method. Background Technology

[0002] Burns are injuries to the skin or mucous membranes caused by boiling water (oil), fire, electricity, light, or chemicals. They can cause local redness, swelling, pain, blisters, and rupture of the skin. In severe cases, they can damage muscles, bones, joints, internal organs, and subcutaneous tissues. Burns are one of the most common injuries in daily work and life. As a common traumatic disease in clinical surgery, most burns are small to medium-sized burns of second degree or lower, causing significant physical and psychological pain and harm to patients.

[0003] Currently, there are no specific drugs or treatments for burns in clinical practice. Western medicine mainly focuses on controlling wound infection, using drugs such as antibiotics and silver salts to reduce inflammation, kill bacteria, and relieve pain. However, Western medicine treatments are time-consuming and struggle to promote wound healing or inhibit the formation of pathological scars. Traditional Chinese medicine (TCM) treatment for burns primarily involves oral and topical application of compound herbal formulas. While TCM formulas are relatively mature and widely used, their therapeutic effects vary, and the combinations and proportions of herbs differ, leading to issues such as large dosages and unclear understanding of the underlying mechanisms, dosage-effect relationships, and mechanisms of action. Therefore, developing a good burn repair ointment could not only alleviate patient suffering and effectively reduce further damage but also reduce scarring, accelerate wound recovery, shorten the treatment period, save treatment costs, and even save patients' lives.

[0004] Sorghum wax is a natural wax secreted by sorghum plants, mainly found on the outer surface of stems, leaves, and grains. Its main components are a mixture of esters composed of higher fatty alcohols and fatty acids, and its physicochemical properties are very similar to carnauba wax. With in-depth research, the development and application of sorghum wax have gradually become more widespread; however, research on its application in treating burns has not yet been reported. Therefore, the development and publication of a green, safe, and non-irritating burn repair preparation using sorghum wax as the active ingredient is of significant value and importance for promoting wound healing after burns, reducing redness and pain, promoting epidermal recovery, and reducing scar formation. Summary of the Invention

[0005] This invention discloses a burn repair ointment with sorghum wax as the active ingredient and its preparation method, aiming to solve the problems existing in the current burn treatment methods and provide a green, safe, non-irritating repair preparation that reduces scarring for burn treatment.

[0006] The first objective of this invention is to provide a burn repair cream with sorghum wax as the active ingredient, the composition and mass percentage of which are: sorghum wax 30%~50%, goosegrass extract 20%~40%, glycerin 20%~30%, anhydrous ethanol 5%~10%, and vitamin C 2%~5%.

[0007] The second objective of this invention is to provide a method for preparing the above-mentioned burn repair ointment, the specific steps of which are as follows: (1) Sorghum wax was melted in a 100°C water bath, and glycerin was added and stirred evenly to obtain mixture 1; (2) Mixture 1 was cooled to 80°C in a water bath, and then the extract of Eleusine indica was added and stirred until homogeneous to obtain mixture 2; (3) Mixture 2 is cooled to 65°C in a water bath, and anhydrous ethanol and vitamin C are added in sequence and stirred evenly. After cooling to room temperature, a burn repair ointment is obtained.

[0008] Furthermore, the preparation method of the sorghum wax is as follows: (1) After the dried sorghum stalks and leaves are crushed and put into a filter bag, the filter bag is pressed into the bottom of a beaker containing 80% ethanol solution with an iron block. The beaker is placed in an ultrasonic cleaner and ultrasonically treated in a water bath at 90°C for 1-2 hours. The filtrate obtained by passing through a 120-mesh sieve is a mixture of sorghum wax extract. (2) The sorghum wax extract mixture was centrifuged at 5000 rpm for 20 min to obtain the supernatant as the sorghum wax extract; (3) The solid precipitated from the sorghum wax extract after standing at 4℃ for 12 hours is dried to obtain sorghum wax.

[0009] Furthermore, the preparation method of the *Eleusine indica* extract is as follows: (1) The dried goosegrass was crushed and passed through a 20-mesh sieve to obtain goosegrass powder; (2) Weigh out the Goosegrass powder, anhydrous ethanol and sterile water and add them in a weight ratio of 1:7:3. Mix well, sonicate at 30℃~50℃ for 30~60 min, and filter through a 0.22μm filter membrane to obtain the Goosegrass extract. (3) The extract of *Eleusine indica* is concentrated into a paste by evaporation in a rotary evaporator at -0.1 MPa and 55°C. This is the extract of *Eleusine indica*.

[0010] The advantages of this invention are as follows: the active ingredient in the burn repair ointment disclosed in this invention is mainly sorghum wax, followed by *Eleusine indica* extract. Sorghum wax has a good therapeutic effect on skin burns, and when mixed with *Eleusine indica*, it produces a positive synergistic effect. The prepared burn repair ointment can promote wound healing, reduce redness and pain, promote epidermal recovery, and reduce scarring. It is a green, safe, and non-irritating burn repair preparation. Implementation

[0011] The principles and features of the present invention are described below with reference to examples. The examples are only used to explain the present invention and are not intended to limit the scope of the present invention. The parts mentioned in the specific embodiments are parts by weight.

[0012] Example 1: A burn repair ointment with sorghum wax as the active ingredient, the preparation steps of which are as follows: (1) Weigh out 30% sorghum wax, 30% goosegrass extract, 30% glycerin, 5% anhydrous ethanol, and 5% vitamin C by weight percentage; (2) The sorghum wax was melted in a 100°C water bath, and after adding glycerin, it was stirred evenly to obtain mixture 1; (3) Mixture 1 was cooled to 80°C in a water bath, and then the extract of Eleusine indica was added and stirred until homogeneous to obtain mixture 2; (4) Mixture 2 is cooled to 65°C in a water bath, and anhydrous ethanol and vitamin C are added in sequence and stirred evenly. After cooling to room temperature, a burn repair cream is obtained. The preparation method of sorghum wax is as follows: (1) After the dried sorghum stalks and leaves are crushed and put into a filter bag, the filter bag is pressed into the bottom of a beaker containing 80% ethanol solution with an iron block. The beaker is placed in an ultrasonic cleaner and ultrasonically treated in a water bath at 90°C for 1 hour. The filtrate obtained by passing through a 120-mesh sieve is a mixture of sorghum wax extract. (2) The sorghum wax extract mixture was centrifuged at 5000 rpm for 20 min to obtain the supernatant as the sorghum wax extract; (3) The sorghum wax extract was allowed to stand at 4℃ for 12 h to precipitate solids, which were then dried to obtain sorghum wax. The preparation method of *Eleusine indica* extract is as follows: (1) The dried goosegrass was crushed and passed through a 20-mesh sieve to obtain goosegrass powder; (2) Weigh out the Goosegrass powder, anhydrous ethanol and sterile water and add them in a weight ratio of 1:7:3. Mix them well, sonicate at 30℃ for 60 min, and filter through a 0.22μm filter membrane to obtain the Goosegrass extract. (3) The extract of *Eleusine indica* is concentrated into a paste by evaporation in a rotary evaporator at -0.1 MPa and 55°C. This is the extract of *Eleusine indica*.

[0013] Example 2: A burn repair ointment with sorghum wax as the active ingredient, the preparation steps of which are as follows: (1) Weigh out 50% sorghum wax, 20% goosegrass extract, 20% glycerin, 8% anhydrous ethanol, and 2% vitamin C by weight percentage; (2) The sorghum wax was melted in a 100°C water bath, and after adding glycerin, it was stirred evenly to obtain mixture 1; (3) Mixture 1 was cooled to 80°C in a water bath, and then the extract of Eleusine indica was added and stirred until homogeneous to obtain mixture 2; (4) Mixture 2 is cooled to 65°C in a water bath, and anhydrous ethanol and vitamin C are added in sequence and stirred evenly. After cooling to room temperature, a burn repair cream is obtained. The preparation method of sorghum wax is as follows: (1) After the dried sorghum stalks and leaves are crushed and put into a filter bag, the filter bag is pressed into the bottom of a beaker containing 80% ethanol solution with an iron block. The beaker is placed in an ultrasonic cleaner and ultrasonically treated in a water bath at 90°C for 2 hours. The filtrate obtained by passing through a 120-mesh sieve is a mixture of sorghum wax extract. (2) The sorghum wax extract mixture was centrifuged at 5000 rpm for 20 min to obtain the supernatant as the sorghum wax extract; (3) The sorghum wax extract was allowed to stand at 4℃ for 12 h to precipitate solids, which were then dried to obtain sorghum wax. The preparation method of *Eleusine indica* extract is as follows: (1) The dried goosegrass was crushed and passed through a 20-mesh sieve to obtain goosegrass powder; (2) Weigh out the Goosegrass powder, anhydrous ethanol and sterile water and add them in a weight ratio of 1:7:3. Mix well, sonicate at 50℃ for 30 min, and filter through a 0.22μm filter membrane to obtain the Goosegrass extract. (3) The extract of *Eleusine indica* is concentrated into a paste by evaporation in a rotary evaporator at -0.1 MPa and 55°C. This is the extract of *Eleusine indica*.

[0014] Example 3: A burn repair ointment with sorghum wax as the active ingredient, the preparation steps of which are as follows: (1) Weigh out 30% sorghum wax, 40% goosegrass extract, 20% glycerin, 7% anhydrous ethanol, and 3% vitamin C by weight percentage; (2) The sorghum wax was melted in a 100°C water bath, and after adding glycerin, it was stirred evenly to obtain mixture 1; (3) Mixture 1 was cooled to 80°C in a water bath, and then the extract of Eleusine indica was added and stirred until homogeneous to obtain mixture 2; (4) Mixture 2 is cooled to 65°C in a water bath, and anhydrous ethanol and vitamin C are added in sequence and stirred evenly. After cooling to room temperature, a burn repair cream is obtained. The preparation method of sorghum wax is as follows: (1) After the dried sorghum stalks and leaves are crushed and put into a filter bag, the filter bag is pressed into the bottom of a beaker containing 80% ethanol solution with an iron block. The beaker is placed in an ultrasonic cleaner and ultrasonically treated in a water bath at 90°C for 1.5 h. The filtrate obtained by passing through a 120-mesh sieve is a mixture of sorghum wax extract. (2) The sorghum wax extract mixture was centrifuged at 5000 rpm for 20 min to obtain the supernatant as the sorghum wax extract; (3) The sorghum wax extract was allowed to stand at 4℃ for 12 h to precipitate solids, which were then dried to obtain sorghum wax. The preparation method of *Eleusine indica* extract is as follows: (1) The dried goosegrass was crushed and passed through a 20-mesh sieve to obtain goosegrass powder; (2) Weigh out the Goosegrass powder, anhydrous ethanol and sterile water and add them in a weight ratio of 1:7:3. Mix well, sonicate at 40℃ for 45 min, and filter through a 0.22μm filter membrane to obtain the Goosegrass extract. (3) The extract of *Eleusine indica* is concentrated into a paste by evaporation in a rotary evaporator at -0.1 MPa and 55°C. This is the extract of *Eleusine indica*.

[0015] Example 4: A burn repair ointment with sorghum wax as the active ingredient, the preparation steps of which are as follows: (1) Weigh out 35% sorghum wax, 25% goosegrass extract, 25% glycerin, 10% anhydrous ethanol, and 5% vitamin C by weight percentage; (2) The sorghum wax was melted in a 100°C water bath, and after adding glycerin, it was stirred evenly to obtain mixture 1; (3) Mixture 1 was cooled to 80°C in a water bath, and then the extract of Eleusine indica was added and stirred until homogeneous to obtain mixture 2; (4) Mixture 2 is cooled to 65°C in a water bath, and anhydrous ethanol and vitamin C are added in sequence and stirred evenly. After cooling to room temperature, a burn repair cream is obtained. The preparation method of sorghum wax is as follows: (1) After the dried sorghum stalks and leaves are crushed and put into a filter bag, the filter bag is pressed into the bottom of a beaker containing 80% ethanol solution with an iron block. The beaker is placed in an ultrasonic cleaner and ultrasonically treated in a water bath at 90°C for 2 hours. The filtrate obtained by passing through a 120-mesh sieve is a mixture of sorghum wax extract. (2) The sorghum wax extract mixture was centrifuged at 5000 rpm for 20 min to obtain the supernatant as the sorghum wax extract; (3) The sorghum wax extract was allowed to stand at 4℃ for 12 h to precipitate solids, which were then dried to obtain sorghum wax. The preparation method of *Eleusine indica* extract is as follows: (1) The dried goosegrass was crushed and passed through a 20-mesh sieve to obtain goosegrass powder; (2) Weigh out the Goosegrass powder, anhydrous ethanol and sterile water and add them in a weight ratio of 1:7:3. Mix well, sonicate at 40℃ for 45 min, and filter through a 0.22μm filter membrane to obtain the Goosegrass extract. (3) The extract of *Eleusine indica* is concentrated into a paste by evaporation in a rotary evaporator at -0.1 MPa and 55°C. This is the extract of *Eleusine indica*.

[0016] Referring to Example 1, an ointment without sorghum wax was prepared by replacing the sorghum wax with an equal amount of sterile water; The remaining technical features are the same as in Example 1.

[0017] Referring to Example 1, an ointment without Goosegrass extract was prepared by replacing the Goosegrass extract with an equal amount of sterile water. The remaining technical features are the same as in Example 1.

[0018] Referring to Example 1, sorghum wax and goosegrass extract were replaced with an equal amount of sterile water to prepare an ointment that did not contain sorghum wax and goosegrass extract. The remaining technical features are the same as in Example 1.

[0019] Fifteen male SD rats, each weighing 200-250 g, were purchased and, after 7 days of acclimatization, their hair was removed from both sides of the spine on their backs, creating an area of ​​approximately 6 cm × 8 cm to serve as the modeling area for burns. A 50 g weight, heated in a boiling water bath for 10 minutes, was placed against the skin of the anesthetized rat modeling area for 10 seconds using tweezers, creating two burn surfaces on each rat, for a total of 30 burn surfaces. All burn surfaces were pathologically confirmed as deep second-degree burns. One hour after the burns, the 15 rats were randomly divided into 5 groups of 3 rats each: control group, Example 1 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group. Each group of rats was treated with the burn repair ointment prepared in the corresponding example, while the control group received no treatment. The ointment was administered twice daily, at 8:00 AM and 8:00 PM, for 21 consecutive days.

[0020] The wound area of ​​each rat was recorded on days 0, 3, 7, 14, and 21 after drug administration. The wound healing rate was calculated as follows: Wound healing rate (%) = (Initial wound area - Wound area at each time point) / Initial wound area × 100%. The results are shown in the table below.

[0021] The results in the table above show that the burn repair ointment prepared in this invention can effectively promote wound healing, with a healing rate of 88.32% after 21 days. The healing rates at all four time points are significant. P＜0.05 The level was higher in the control group and comparative groups 1 and 3, while comparative group 2 was significantly higher. P＜0.05 The results were higher than those of the control group, comparative example 1, and comparative example 3. Comparative example 1 and comparative example 3 showed no significant changes compared to the control group, proving that the main effective ingredient in the burn repair ointment of this invention is sorghum wax, while the extract of goosegrass and the other ingredients in the burn repair ointment have a synergistic effect. The burn prevention and treatment ointment prepared by mixing has an unexpected effect that cannot be achieved by a single substance. It is not a simple superposition of several substances and their functions, but a new type of burn repair ointment with significant effects.

[0022] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A burn repair ointment with sorghum wax as the active ingredient, characterized in that, It is a mixture of the following components by weight: 30%~50% sorghum wax, 20%~40% goosegrass extract, 20%~30% glycerin, 5%~10% anhydrous ethanol, and 2%~5% vitamin C.

2. The burn repair ointment with sorghum wax as the active ingredient according to claim 1, characterized in that, The preparation method of the sorghum wax is as follows: (1) After the dried sorghum stalks and leaves are crushed and put into a filter bag, the filter bag is pressed into the bottom of a beaker containing 80% ethanol solution with an iron block. The beaker is placed in an ultrasonic cleaner and ultrasonically treated in a water bath at 90°C for 1-2 hours. The filtrate obtained by passing through a 120-mesh sieve is a mixture of sorghum wax extract. (2) The sorghum wax extract mixture was centrifuged at 5000 rpm for 20 min to obtain the supernatant as the sorghum wax extract; (3) The solid precipitated from the sorghum wax extract after standing at 4℃ for 12 hours is dried to obtain sorghum wax.

3. The burn repair ointment with sorghum wax as the active ingredient according to claim 1, characterized in that, The preparation method of the *Eleusine indica* extract is as follows: (1) The dried goosegrass was crushed and passed through a 20-mesh sieve to obtain goosegrass powder; (2) Weigh out the Goosegrass powder, anhydrous ethanol and sterile water and add them in a weight ratio of 1:7:

3. Mix well, sonicate at 30℃~50℃ for 30~60 min, and filter through a 0.22μm filter membrane to obtain the Goosegrass extract. (3) The extract of *Eleusine indica* is concentrated into a paste by evaporation in a rotary evaporator at -0.1 MPa and 55°C. This is the extract of *Eleusine indica*.

4. The burn repair ointment with sorghum wax as the active ingredient according to claim 1, characterized in that, The preparation steps of the burn repair cream are as follows: (1) Sorghum wax was melted in a 100°C water bath, and glycerin was added and stirred evenly to obtain mixture 1; (2) Mixture 1 was cooled to 80°C in a water bath, and then the extract of Eleusine indica was added and stirred until homogeneous to obtain mixture 2; (3) Mixture 2 is cooled to 65°C in a water bath, and anhydrous ethanol and vitamin C are added in sequence and stirred evenly. After cooling to room temperature, a burn repair ointment is obtained.

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