Bactericide for preventing and treating diseases of food crop field

Abstract

The invention belongs to the technical field of bactericides, and particularly relates to a bactericide for preventing and treating diseases of a food crop field. The invention relates to a bactericide, wherein the active component of the bactericide is prepared by compounding vanillyl thiodithioether and tebuconazole or benzovindiflupyr according to the mass ratio of (1-150): (150-1). The active ingredients of the bactericide are formed by compounding vanillyl thiodithiolate and tebuconazole or benzovindiflupyr, and the compounded combinations have a synergistic effect on pathogenic bacteria causing diseases of the food crop field, so that the prevention and treatment effect on the diseases of the food crop field can be improved, the dosage can be reduced, the prevention and treatment cost can be reduced, and the bactericide has a good application prospect in prevention and treatment of the diseases of the food crop field. The development speed of drug resistance of pathogenic bacteria is delayed, and the composition is of great significance to development of bactericides with synergistic effects on diseases of food crop fields.

Description

Technical Field

[0001] This invention belongs to the field of fungicide technology, specifically relating to a fungicide for preventing and controlling diseases in grain crops. Background Technology

[0002] The safe production of grain crops is directly related to agricultural economic development and food supply security. Diseases caused by fungal pathogens are one of the important factors restricting the yield and quality of grain crops, among which Fusarium graminearum (Fusarium graminearum) is a significant contributor. Fusarium gramineae Diseases caused by fungal infections such as wheat and corn are widespread in grain-producing areas, and these diseases have a serious impact on various grain crops.

[0003] Currently, chemical agents remain the primary means of controlling these fungal diseases. Chemical agents offer advantages such as rapid effectiveness, broad coverage, and ease of application, and various fungicidal agents have been developed in related fields. However, long-term continuous use of a single fungicide can easily lead to drug resistance in pathogens, causing the control effect to gradually decline or even become ineffective. This necessitates continuously increasing the dosage or changing the agent, which not only increases the control components but also exacerbates environmental pressure and further intensifies the development of drug resistance.

[0004] Combining different fungicidal active ingredients is one of the effective and convenient ways to develop new pesticides and control resistant pathogens in grain crops. Among these, synergistic formulations can significantly improve efficacy, reduce pesticide dosage, and slow down the development of pathogen resistance. Therefore, developing fungicides with synergistic effects against diseases in grain crops is of great significance.

[0005] Vanillin thiocyanate (CAS: 2088490-79-1, molecular formula C) 19 H 23 O4ClS2 is a small-molecule pesticide independently developed by the State Key Laboratory of Green Pesticides and Agricultural Biotechnology at Guizhou University. It is a plant-derived pesticide with vanillin as the lead compound, and it poses no threat to the ecological environment or food safety. The study "Inhibitory Activity of Vanillin on Rice Blast and its Application in Rice" discloses that vanillin has a significant impact on the mycelial growth, molecular spore production, spore germination, and appressorium formation of rice blast fungus. Existing technologies also disclose that vanillin, when combined with citric acid, sodium alginate oligosaccharides, or allicin, has a synergistic effect in controlling plant viral diseases; that vanillin, when combined with copper rosinate, has a synergistic effect on bacterial and viral diseases of crops; and that vanillin, when combined with Bacillus subtilis, Trichoderma harzianum, or Bacillus subtilis, has a synergistic effect on the anthracnose pathogen of dragon fruit. However, there are currently no reports on the combination of vanillin with tebuconazole or benzo[a]fluconazole. Summary of the Invention

[0006] The purpose of this invention is to provide a fungicide for controlling diseases in grain crops, thereby solving the problems associated with using a single fungicide such as vanillin to control diseases in grain crops.

[0007] To achieve the above objectives, the present invention provides the following technical solution: A fungicide, wherein the active ingredient of the fungicide is a compound of vanillin and tebuconazole or benzoyl permethrin in a mass ratio of 1-150:150-1.

[0008] Preferably, the mass ratio of vanillin to tebuconazole is 1-7:40-1.

[0009] Preferably, the mass ratio of vanillin to tebuconazole is 1:15.

[0010] Preferably, the mass ratio of the compound of vanillin to benzoyl permethrin is 1:25-1.

[0011] Preferably, the active ingredient accounts for 0.75-65% of the total mass of the fungicide by weight, with the remainder being auxiliary ingredients permitted for pesticide addition.

[0012] The present invention also provides the application of the fungicide in the prevention and control of field diseases in grain crops.

[0013] As a priority, the field diseases of the grain crops include those caused by Fusarium graminearum (…). Fusarium gramineae Diseases caused by infection.

[0014] Compared with the prior art, the present invention has the following beneficial effects: The active ingredient of this fungicide is a compound of vanillin and either tebuconazole or benzimidazole. The inhibitory effects of each single agent and the compound combination on the mycelial growth of pathogens were determined using the mycelial rate method. The Sun Yunpei co-toxicity coefficient was used to evaluate the synergistic effects of the compound combination on the pathogens. The results showed that when the mass ratio of vanillin to tebuconazole was 1:7:40:1, and when the mass ratio of vanillin to benzimidazole was 1:25:1, each compound combination exhibited a synergistic effect against pathogens causing diseases in grain crops. This improved the control effect on grain crop diseases, helped reduce pesticide dosage and control costs, and slowed the development of pathogen resistance. This is of great significance for developing fungicides with synergistic effects against grain crop diseases. Detailed Implementation

[0015] The technical solution of this invention patent will be clearly and completely described below. Obviously, the described embodiments are some embodiments of this invention, but not all embodiments.

[0016] 1. Materials and Methods 1.1 Test strains Fusarium graminearum ( Fusarium gramineae The spores were isolated from wheat plants in the suburbs of Zhengzhou, Henan Province, which had shown typical symptoms of stem rot, and were preserved on PDA medium.

[0017] PDA culture medium formula: 200g peeled potato, 20g glucose, 15g agar, 1000mL distilled water, natural pH value.

[0018] 1.2 Test reagents 98% vanillin-thiophanate-methyl technical, 95% tebuconazole technical, 96% benzo[a]flufenicol technical The test reagent was dissolved and diluted with 0.1% Tween-80 aqueous solution to form a single-agent stock solution. Based on the results of previous tests, multiple formulations were set up. Each single-agent stock solution and each formulation mixture were then diluted with 0.1% Tween-80 aqueous solution, and five mass concentration gradients were set up.

[0019] 1.3 Test Methods The toxicity of vanillin, tebuconazole, and benzo[a]fluconazole, as well as their mixtures, to the tested strain Fusarium graminearum was determined using the mycelial growth rate method.

[0020] The pre-melted and cooled PDA medium to approximately 50°C was mixed with the drug solution in a conical flask at a volume ratio of 9:1. The mixture was then poured into 9cm diameter petri dishes, 10mL per dish, and allowed to cool to form drug-containing plates. A 0.1% Tween-80 aqueous solution was used as a blank control. Each treatment was replicated four times, with three plates per replicate.

[0021] The tested strain *Fusarium graminearum* was inoculated into PDA medium and cultured at a constant temperature of 25°C. After propagation, 6 mm diameter mycelial discs were cut along the edge of the colonies using a punch. One mycelial disc was inoculated into the center of each of the drug-containing and blank control plates. The plates were then capped and transferred to a 25°C incubator. When the diameter of the blank control colonies reached approximately 7 cm, the colony diameter was measured using the cross-sectional method, and the inhibition rate of mycelial growth by different treatments was calculated.

[0022]

[0023] 1.4 Data Analysis Linear regression was performed with the logarithm of the fungicide concentration as x and the corresponding probability of mycelial growth inhibition as y to obtain the virulence regression equation and the EC50 of the fungicide on the tested strains. 50 The cotoxicity coefficient (CTC) was calculated using the Sun Yunpei method.

[0024]

[0025] In the above formula: AT --The measured toxicity index of the mixture; S --EC of standard reagents 50 The unit is mg / L; M --EC mixture 50 The unit is mg / L.

[0026]

[0027] In the above formula: THIRTY --Theoretical toxicological index of the mixture; YOU A --Toxicity index of drug A; P A --Percentage content of drug A in the mixture, expressed as percentage (%). YOU B --Toxicity index of drug B; P B --The percentage content of drug B in the mixture, expressed as a percentage (%).

[0028]

[0029] In the above formula: CTC --Cotoxicity coefficient; AT --Measured toxicity index of the mixture; THIRTY --Theoretical toxicity index of mixed formulations.

[0030] 1.5 Pharmaceutical Evaluation The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC). CTC ≤ 80 indicates antagonistic effect, 80 < CTC < 120 indicates additive effect, and CTC ≥ 120 indicates synergistic effect. The results are shown in Table 1-3.

[0031]

[0032] Table 1 shows that vanillin, tebuconazole, and benzovindiflubenzuron all have inhibitory effects on the tested strain *Fusarium graminearum*, and their EC50 values ​​are [not specified]. 50 The values ​​were 26.5439 mg / L, 1.0326 mg / L and 4.1047 mg / L, respectively.

[0033]

[0034] Table 2 shows that when the mass ratio of vanillin to tebuconazole is 1-7:40-1, the co-toxicity coefficient of the compound against the tested strain *Fusarium graminearum* is 121.1893-195.1663, which is greater than 120, indicating a synergistic effect. The synergistic effect is most significant when the mass ratio is 1:15. Therefore, the compound combination of vanillin and tebuconazole in this invention can improve the control effect against diseases of grain crops caused by *Fusarium graminearum*, helping to reduce pesticide dosage, lower control costs, and slow down the development of drug resistance in the pathogen.

[0035]

[0036] Table 3 shows that when the mass ratio of vanillin to benzalkonium chloride is 1:25-1, the co-toxicity coefficient of the compound against the tested strain *Fusarium graminearum* is 121.7018-168.2254, which is greater than 120, indicating a synergistic effect. Therefore, the compound combination of vanillin and benzalkonium chloride of this invention can improve the control effect against diseases of grain crops caused by *Fusarium graminearum*, helping to reduce pesticide dosage, lower control costs, and slow down the development of drug resistance in the pathogen.

Claims

1. A bactericide, characterized in that, The active ingredient of the fungicide is a compound of vanillin thiocyanate and tebuconazole or benzoxopyram at a mass ratio of 1-150:150-1.

2. The bactericide according to claim 1, characterized in that, The mass ratio of vanillin thiocyanate to tebuconazole is 1-7:40-1.

3. The bactericide according to claim 2, characterized in that, The mass ratio of vanillin thiocyanate to tebuconazole is 1:

15.

4. The bactericide according to claim 1, characterized in that, The mass ratio of vanillin thiocyanate to benzoyl permethrin is 1:25-1.

5. The bactericide according to claim 1, characterized in that, The active ingredient accounts for 0.75-65% of the total mass of the fungicide by weight, with the remainder being auxiliary ingredients permitted for addition in pesticide science.

6. The application of the fungicide according to any one of claims 1-5 in the prevention and control of field diseases in grain crops.

7. The application according to claim 6, characterized in that, The diseases affecting grain crops include those caused by Fusarium graminearum (… Fusarium graminearum Diseases caused by infection.

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