Heart perfusion device for small and medium-sized animals

Abstract

The invention relates to the field of perfusion devices, and discloses a small and medium-sized animal heart perfusion device which comprises an injection device and an operation device, the injection device comprises a shell, a needle head telescopically arranged in the shell and a suction cup arranged on the shell, and the suction cup is used for being adsorbed to the heart of an animal; the perfusion operation of the animal heart is completed on the operation device. The sucking disc is adsorbed on the animal heart, so that the needle head can be prevented from falling off along with beating of the animal heart, and the problems of perfusate leakage, low perfusion efficiency and the like caused by multiple holes and loopholes of the heart due to repeated insertion of the needle head are solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of perfusion devices, in particular to a small and medium-sized animal heart perfusion device. BACKGROUND

[0002] In order to carry out effective tissue section analysis, the animal is fixed by heart perfusion and the tissue sample is taken, and the steps are as follows: the chest wall of the small and medium-sized animal is opened along the xiphoid and the lower edge of the rib, the front chest wall is clamped with a hemostat, the heart is exposed by lifting up, a needle is punctured into the left ventricle, the needle and the chest wall are fixed together with glue to prevent the needle from moving due to the beating of the heart, then the right auricle is cut, a syringe is connected to the punctured needle, and physiological saline solution and paraformaldehyde solution are slowly injected to replace the blood until clear liquid flows out of the right auricle; after the perfusion is completed, the perfusion instrument is removed, and the target organs are separated in turn.

[0003] However, this method has the following problems: (1) high complexity of operation: after the front chest wall is lifted, there is still inertia to cover the chest cavity, and additional manpower is needed to keep the chest wall in an open state all the time, which increases the complexity of operation and the required manpower; (2) difficulty in fixing the needle: fixing the needle with glue is easy to make the needle come out with the beating of the heart, and repeatedly inserting the needle will cause multiple holes in the heart, which will cause problems such as leakage of perfusion fluid and low perfusion efficiency; (3) difficulty in controlling the depth of needle insertion: if the needle is inserted too deep, the perfusion fluid will enter the pulmonary circulation; if it is inserted too shallow, it cannot pierce the heart wall; (4) contamination of the operation table: after the blood and perfusion fluid flow out of the right auricle, the operation table will be contaminated, affecting the vision and experimental environment, interfering with the subsequent operation, and increasing the risk of specimen contamination; (5) complicated perfusion procedure: when the syringe is used to inject the perfusion fluid, it needs to be replaced several times. This process requires frequent replacement of the syringe, consumes a long time, and in the later stage of perfusion, the blood of small animals coagulates slightly, which increases the pressure required for perfusion, further increasing the difficulty of operation for the experimental personnel. SUMMARY

[0004] The purpose of the present application is to provide a small and medium-sized animal heart perfusion device to solve at least one of the problems existing in the prior art, and the present application adopts the following technical scheme:

[0005] The small and medium-sized animal heart perfusion device of the present application comprises an injection device, which comprises:

[0006] a shell;

[0007] a needle, which is telescopically arranged in the shell;

[0008] a suction cup, which is arranged on the shell for adsorbing on the heart of the animal.

[0009] Further, the suction cup is located at the front end of the shell, and the suction cup is connected with the shell through a flexible arm.

[0010] Still further, the flexible arm is a hollow structure, one end of the hollow cavity of the flexible arm is communicated with the suction cup, and the other end is communicated with the air inlet of a negative pressure pump, and the negative pressure pump is arranged in the shell.

[0011] Still further, a negative pressure button is arranged on the outer wall of the shell, and the negative pressure button is electrically connected with the negative pressure pump.

[0012] Still further, the tail of the needle is communicated with a liquid inlet pipe, and the tail end of the liquid inlet pipe is communicated with a liquid supply assembly.

[0013] The liquid supply assembly comprises:

[0014] A bottle body one is used for containing physiological saline solution;

[0015] A bottle body two is used for containing polyformaldehyde solution;

[0016] A three-way valve, the bottle body one and the bottle body two are respectively communicated with the three-way valve;

[0017] A liquid supply pipe one, one end of which is communicated with the three-way valve, and the other end of which is communicated with the tail end of the liquid inlet pipe;

[0018] A liquid supply pump is arranged on the liquid supply pipe one.

[0019] Still further, a plurality of liquid supply pipes two are communicated with the liquid supply pipe one, and the plurality of liquid supply pipes two are located between the liquid supply pump and the liquid inlet pipe.

[0020] Still further, a groove is arranged on the shell, a through hole is arranged on the front end of the shell and communicated with the groove, the needle is arranged in the groove, and the needle is arranged corresponding to the through hole; a linear driving component is arranged in the shell, and the action end of the linear driving component is connected with the tail end of the needle.

[0021] Still further, a needle pushing button is arranged on the outer wall of the shell, and the needle pushing button is electrically connected with the linear driving component.

[0022] Still further, an operating device is further included, the operating device comprises an operating platform, a fixed connection baffle one is arranged on the top surface of the operating platform, three U-shaped baffles two are slidably connected to one side of the baffle one, and adjacent baffles two and the baffle one and adjacent two baffles two are connected through telescopic connecting pieces; elastic ropes for fixing animal limbs are arranged on the three baffles two, and a fixing assembly for fixing animal chest walls is arranged on the baffle one.

[0023] Further, a sewage collecting tank is arranged below the operation platform, the top surface of the sewage collecting tank is in sealing connection with the bottom surface of the operation platform, a vacuum pump is arranged outside the sewage collecting tank and is in communication with the inside of the sewage collecting tank, and a plurality of sewage discharge holes are arranged on the operation platform and are in communication with the inside of the sewage collecting tank.

[0024] Compared with the prior art, the present application has the beneficial technical effects that:

[0025] By adsorbing the suction cup on the animal heart, the needle can be prevented from being pulled out with the beating of the animal heart, and the problems of perfusion liquid leakage and low perfusion efficiency caused by repeatedly inserting the needle to cause multiple holes in the heart can be avoided. BRIEF DESCRIPTION OF DRAWINGS

[0026] The present application will be further described below in conjunction with the drawings.

[0027] Figure 1 It is a schematic diagram of the three-dimensional structure of the injection device of the present application;

[0028] Figure 2 It is a schematic diagram of the three-dimensional structure of the liquid supply assembly of the present application;

[0029] Figure 3 It is a schematic diagram of the three-dimensional structure of the operation device of the present application;

[0030] Figure 4 It is a schematic diagram of the three-dimensional structure of the baffle two of the present application;

[0031] Figure 5 It is a schematic diagram of the three-dimensional structure of the fixing assembly of the present application.

[0032] The drawings are described as follows: 1, injection device; 11, shell; 111, groove; 12, needle; 13, suction cup; 14, flexible arm; 15, negative pressure button; 16, needle pushing button; 17, liquid inlet pipe; 18, liquid supply assembly; 181, bottle one; 182, bottle two; 183, three-way valve; 184, liquid supply pipe one; 185, liquid supply pump; 186, liquid supply pipe two; 187, flow sensor; 188, control valve; 2, operation device; 21, operation platform; 211, mounting groove; 212, guide slide rail; 213, sewage discharge hole; 22, baffle one; 23, baffle two; 231, guide pulley; 24, telescopic connecting piece; 25, elastic rope; 26, fixing assembly; 261, mounting column; 262, spring; 263, chest wall clamp; 27, sewage collecting tank; 28, vacuum pump; 29, sewage discharge pipe; 30, sewage discharge valve. DETAILED DESCRIPTION

[0033] To make the technical problems, technical solutions, and beneficial effects of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments.

[0034] This embodiment discloses a small to medium-sized animal heart perfusion device, including an injection device 1 and an operating device 2.

[0035] like Figures 1-2 As shown, the injection device 1 includes a housing 11, a needle 12, and a suction cup 13. The needle 12 is retractably disposed in the housing 11. The suction cup 13 is disposed on the housing 11. The suction cup 13 is used to adsorb and fix the animal heart. Multiple suction cups 13 can be provided. In this embodiment, four suction cups 13 are provided.

[0036] By using this method, the suction cup 13 is attached to the animal's heart, preventing the needle 12 from falling out with the heartbeat and avoiding damage to the animal's heart caused by reinserting the needle 12.

[0037] In a further optimized design, the suction cup 13 is located at the front end of the housing 11. The suction cup 13 is connected to the housing 11 via a flexible arm 14, with the flexible arm 14 and the suction cup 13 arranged in a one-to-one correspondence. The flexible arm 14 has a hollow structure, with one end of the hollow inner cavity of the flexible arm 14 connected to the suction cup 13 and the other end connected to the air inlet of the negative pressure pump. The negative pressure pump is fixedly connected in the housing 11. A negative pressure button 15 is provided on the outer wall of the housing 11, and the negative pressure button 15 is electrically connected to the negative pressure pump.

[0038] Using this method, after the needle 12 is inserted into the heart, the negative pressure pump can be controlled by the negative pressure button 15 to extract the air from the middle of the suction cup 13, so that the suction cup 13 can be stably attached to the heart.

[0039] In a further optimized design, the housing 11 has a groove 111, and the front end of the housing 11 has a through hole that communicates with the groove 111. The needle 12 is disposed in the groove 111 and is disposed corresponding to the through hole. A linear drive component is disposed inside the housing 11, and the actuating end of the linear drive component is connected to the tail end of the needle 12. The linear drive component is configured as an electric push rod. A push button 16 is disposed on the outer wall of the housing 11, and the push button 16 is electrically connected to the linear drive component.

[0040] Using this solution, the linear drive component can be controlled by the push button 16 to extend its moving end, which can drive the needle 12 to move through the through hole on the housing 11 to the outside of the housing 11, thus achieving the effect of inserting it into the animal's heart.

[0041] Further optimization scheme, the tail end of the needle 12 is provided with a pressure sensor at the connection of the linear driving part, and the pressure sensor is used for detecting the pressure received by the needle 12. When the needle 12 pierces the heart wall, the pressure gradually increases, and when the needle 12 penetrates the heart wall, the pressure drops suddenly, and the needle 12 can stop the needle insertion.

[0042] With this scheme, the effect of controlling the needle insertion depth of the needle 12 can be realized.

[0043] Further optimization scheme, the needle 12 is provided with a scale line, the scale line is arranged along the length direction of the needle 12; the tail end of the needle 12 is further provided with a distance sensor, the distance sensor is used for detecting the needle insertion depth of the needle 12; the distance sensor is arranged as a laser ranging sensor, when the needle 12 moves, the laser ranging sensor will move synchronously with the needle 12, the laser of the laser ranging sensor hits the inner wall of the groove 111 along the moving direction of the needle 12, that is, the needle insertion depth of the needle 12 can be calculated.

[0044] With this scheme, the experimental personnel can determine the depth of the needle 12 into the heart of the animal again.

[0045] It should be noted that the pressure sensor and the distance sensor are prior art, and the working principle and the use method are known, which will not be repeated here.

[0046] Further optimization scheme, the tail of the needle 12 is communicated with a liquid inlet pipe 17, the tail end of the liquid inlet pipe 17 is communicated with a liquid supply assembly 18, the liquid inlet pipe 17 is arranged as a flexible hose which can be elongated or shortened when the needle 12 moves; the liquid supply assembly 18 includes a bottle body one 181 for containing physiological saline solution, a bottle body two 182 for containing polyformaldehyde solution, a three-way valve 183, a liquid supply pipe one 184 and a liquid supply pump 185, the bottle body one 181 and the bottle body two 182 are communicated with the three-way valve 183 respectively, one end of the liquid supply pipe one 184 is communicated with the three-way valve 183, and the other end is communicated with the tail end of the liquid inlet pipe 17; the liquid supply pump 185 is installed on the liquid supply pipe one 184.

[0047] With this scheme, the physiological saline solution and the polyformaldehyde solution can be delivered into the heart of the animal respectively, and the complex procedure of multiple liquid and syringe replacement when the syringe is used to inject the perfusion solution is solved, and the time is saved.

[0048] Further optimization scheme, a plurality of liquid supply pipes two 186 are communicated on the liquid supply pipe one 184, and the plurality of liquid supply pipes two 186 are located between the liquid supply pump 185 and the liquid inlet pipe 17.

[0049] With this scheme, the effect of automatic perfusion of multiple animals at the same time can be realized, the operation efficiency is improved, and the time is saved.

[0050] The scheme is further optimized. Flow sensors 187 and control valves 188 are installed on both the second liquid supply pipe 186 and the first liquid supply pipe 184. The flow sensor 187 and control valve 188 on the first liquid supply pipe 184 are located between the second liquid supply pipe 186 and the inlet pipe 17. The three-way valve 183, the liquid supply pump 185, the flow sensor 187, and the control valve 188 are electrically connected to the controller.

[0051] The working process of the above technical solution is as follows: When perfusing an animal's heart, the controller first controls the opening direction of the three-way valve 183 to keep the saline solution flowing smoothly into the animal's heart. Then, the controller starts the supply pump 185 to infuse the saline solution into the animal's heart. The flow sensor 187 measures the flow rate. When the delivered saline solution reaches a preset value, the flow sensor 187 sends a signal to the controller. After receiving the signal from the flow sensor 187, the controller controls the opening direction of the three-way valve 183 to keep the paraformaldehyde solution flowing smoothly into the heart. Paraformaldehyde solution is infused into the animal's heart, and flow sensor 187 continues to measure the flow rate. When the infused paraformaldehyde solution reaches a preset value, flow sensor 187 sends a signal to the controller. After receiving the signal from flow sensor 187, the controller controls the opening direction of three-way valve 183 and infuses physiological saline solution into the animal's heart. Flow sensor 187 continues to measure the flow rate. When the preset value for completion of infusion is reached, flow sensor 187 sends a signal to the controller. After receiving the signal from flow sensor 187, the controller stops the infusion pump 185.

[0052] It should be noted that the three-way valve 183, the liquid supply pump 185, the flow sensor 187, the control valve 188, and the controller are all existing technologies, and their working principles and usage methods are known, so they will not be described in detail here.

[0053] like Figures 3-5 As shown, the animal heart perfusion procedure is performed on the operating device 2. The operating device 2 includes an operating platform 21, a fixedly connected baffle 22 on the top surface of the operating platform 21, and three U-shaped baffles 23 slidably connected to one side of the baffle 22. Adjacent baffles 23 are connected to the baffle 22, and adjacent baffles 23 are connected to each other via telescopic connectors 24. Each of the three baffles 23 is equipped with elastic ropes 25 for fixing the animal's limbs, and the baffle 22 is equipped with a fixing component 26 for fixing the animal's chest wall.

[0054] Specifically, the inner side of the second baffle 23 is set as an inclined surface, and the two ends of the elastic rope 25 are respectively fixedly connected to the inclined surface of the second baffle 23, so that the elastic rope 25 is in a taut state. The telescopic connector 24 is made of plastic, which can be stretched or shortened under the action of external force.

[0055] This design, through the combination of baffle 1 22, baffle 23, and telescopic connector 24, forms a closed structure that can block the liquid overflowing during injection; at the same time, the size of the closed area can be adjusted to enable injection operations for different sizes of movements, thus improving applicability.

[0056] In a further optimized design, the fixing component 26 includes a mounting post 261 fixedly connected to the first baffle 22. A spring 262 is connected to the mounting post 261, and a chest wall clamp 263 is connected to the end of the spring 262. During operation, the animal is placed in the closed area formed by the first baffle 22, the second baffle 23, and the telescopic connector 24, with the animal's head facing away from the first baffle 22. The animal's limbs are then fixed using the elastic ropes 25 on the second baffle 23. After opening the animal's cavity, the chest wall of the animal is lifted upwards towards the first baffle 22 using the chest wall clamp 263.

[0057] It should be noted that the chest wall clip 263 is existing technology, and its working principle and usage are known, so it will not be described in detail here.

[0058] To further optimize the design, each baffle 23 has a mounting groove 211 located below it, on the top surface of the operating platform 21. A guide rail 212 is fixedly installed in the mounting groove 211, and a guide wheel 231 is installed on the bottom surface of the baffle 23. The baffle 23 is slidably connected to the top surface of the operating platform 21 via the guide wheel 231 and the guide rail 212. Furthermore, to ensure that the baffle 23 and the telescopic connector 24 continue to provide a shielding effect against the injected liquid, the bottom surfaces of the baffle 23 and the telescopic connector 24 are flush with the top surface of the operating platform 21.

[0059] To further optimize the design, a sewage collection tank 27 is provided below the operating platform 21, and the top surface of the sewage collection tank 27 is sealed to the bottom surface of the operating platform 21; a vacuum pump 28 is provided on the outside of the sewage collection tank 27 and communicates with its interior, and the operating platform 21 is provided with multiple sewage discharge holes 213 that communicate with the interior of the sewage collection tank 27.

[0060] This solution uses a vacuum pump 28 to extract air from the sewage collection tank 27, creating a negative pressure environment inside the sewage collection tank 27. This allows dirt on the operating platform 21 to quickly flow into the sewage collection tank 27 through the drain hole 213, keeping the top surface of the operating platform 21 clean.

[0061] To further optimize the design, in order to facilitate the discharge of sewage from the sewage collection tank 27, a drain pipe 29 is fixedly connected to the bottom of one side of the sewage collection tank 27, and a drain valve 30 is installed on the drain pipe 29.

[0062] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A small to medium-sized animal heart perfusion device, characterized in that: Includes an injection device (1), said injection device (1) comprising: Shell (11); The needle (12) is retractably disposed in the housing (11); The suction cup (13) is provided on the housing (11) for adsorbing onto the animal heart.

2. The small and medium-sized animal heart perfusion device according to claim 1, characterized in that: The suction cup (13) is located at the front end of the housing (11), and the suction cup (13) is connected to the housing (11) via a flexible arm (14).

3. The small and medium-sized animal heart perfusion device according to claim 2, characterized in that: The flexible arm (14) has a hollow structure. One end of the hollow cavity of the flexible arm (14) is connected to the suction cup (13), and the other end is connected to the air inlet of the negative pressure pump. The negative pressure pump is installed in the housing (11).

4. The small and medium-sized animal heart perfusion device according to claim 3, characterized in that: A negative pressure button (15) is provided on the outer wall of the housing (11), and the negative pressure button (15) is electrically connected to the negative pressure pump.

5. The small and medium-sized animal heart perfusion device according to claim 1, characterized in that: The tail of the needle (12) is connected to the liquid inlet tube (17), and the tail end of the liquid inlet tube (17) is connected to the liquid supply assembly (18). The liquid supply assembly (18) includes: Bottle 1 (181) is used to hold physiological saline solution; Bottle 2 (182) is used to hold paraformaldehyde solution; A three-way valve (183) is connected to the first bottle body (181) and the second bottle body (182), respectively. One end of the liquid supply pipe (184) is connected to the three-way valve (183), and the other end is connected to the tail end of the liquid inlet pipe (17). A liquid supply pump (185) is installed on the liquid supply pipe (184).

6. The small and medium-sized animal heart perfusion device according to claim 5, characterized in that: The first liquid supply pipe (184) is connected to multiple second liquid supply pipes (186), and all of the second liquid supply pipes (186) are located between the liquid supply pump (185) and the inlet pipe (17).

7. The small and medium-sized animal heart perfusion device according to claim 1, characterized in that: The housing (11) is provided with a groove (111), and the front end of the housing (11) is provided with a through hole that communicates with the groove (111). The needle (12) is disposed in the groove (111) and is disposed corresponding to the through hole. A linear drive component is disposed inside the housing (11), and the actuating end of the linear drive component is connected to the tail end of the needle (12).

8. The small and medium-sized animal heart perfusion device according to claim 7, characterized in that: A push button (16) is provided on the outer wall of the housing (11), and the push button (16) is electrically connected to the linear drive component.

9. The small and medium-sized animal heart perfusion device according to claim 1, characterized in that: It also includes an operating device (2), which includes an operating platform (21). The top surface of the operating platform (21) is fixedly connected to a baffle (22). Three baffles (23) arranged in a U-shape are slidably connected to one side of the baffle (22). Adjacent baffles (23) are connected to the baffle (22) and adjacent baffles (23) are connected by telescopic connectors (24). Each of the three baffles (23) is provided with an elastic rope (25) for fixing the animal's limbs. The baffle (22) is provided with a fixing component (26) for fixing the animal's chest wall.

10. The small-to-medium-sized animal heart perfusion device according to claim 9, characterized in that: Below the operating platform (21) is a sewage collection tank (27), the top surface of the sewage collection tank (27) is sealed to the bottom surface of the operating platform (21); a vacuum pump (28) is provided on the outside of the sewage collection tank (27) and communicates with its interior; and the operating platform (21) is provided with a plurality of sewage discharge holes (213) communicating with the interior of the sewage collection tank (27).

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