Molecular-nerve dual-channel synergistic targeting barrier repair composition and application thereof

Abstract

The invention relates to a molecule-nerve dual-channel synergistic targeting barrier repair composition and application thereof, the molecule-nerve dual-channel synergistic targeting barrier repair composition comprises a functional component composition of a saffron crocus extract, a ginseng extract and a polianthes tuberose extract in a mass ratio of (0.01-1): (0.01-1.5): (0.01-0.5), and the saffron crocus extract and the ginseng extract can also be wrapped in lipidosome. The composition is used in skin care products. Compared with the prior art, the invention constructs a skin and emotion regulation combination system taking the saffron crocus extract, the ginseng extract and the tuberose extract as main components, improves the skin state from multiple dimensions, and has remarkable advantages.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics and personal care, in particular to a molecular-neural dual pathway synergistic targeted barrier repair composition and its application. In the field of cosmetics and personal care, the composition can achieve the effects of improving skin dullness, reducing stress and relieving tension. BACKGROUND

[0002] Chronic stress is becoming an "invisible killer" affecting people's physical and mental health, mainly manifested in changes in skin state, such as stress wrinkles, depression facial redness, and anxiety skin dullness. In response to this emotional skin state problem, consumers have also begun to realize the nature of the problem and seek skin care products that can both regulate emotion-induced neuroendocrine disorders and improve the external appearance of the skin.

[0003] In recent years, the field of skin neurology has developed continuously, revealing the internal connection between emotions and skin problems, breaking the traditional understanding of the causes of skin problems, and providing a new direction and solution path for skin care and improving skin state. So far, a considerable number of skin and emotion regulation products have been put into use and have achieved relatively good results. For example, TRPV1 inhibitors are used as effective ingredients to inhibit the binding of allergic stimuli or nerve signals to receptors and block the deep damage of stress hormones to the skin.

[0004] However, the existing technical solutions still face three major problems: (1) It is difficult to balance stress regulation and structure repair. Some products have obvious short-term soothing and improvement effects, but lack of skin structure repair, which is limited: existing skin care products that improve skin state from the perspective of regulating emotions mainly use specific functional ingredients to act on skin local nerve receptors or related signal molecules, thereby reducing local neurogenic inflammatory reactions caused by external stress stimuli or reducing skin sensitivity to stimuli. This action helps to relieve skin discomfort caused by emotional stress (such as redness, stinging, and itching), achieving soothing effects. For example, the invention patent CN118304233A mainly focuses on inhibiting the damage of excessive secretion of stress factor cortisol to the skin, achieving the purpose of short-term soothing of skin sensitivity. Due to the lack of skin structure repair ingredients, the skin state cannot be fundamentally improved, and once the product is stopped, it is easy to have a regression phenomenon, and the long-term repair effect is relatively limited.

[0005] (2) The skin regulation effect dimension is relatively single, and lacks multi-aspect regulation of the neuroendocrine system, resulting in that the negative influence caused by emotion cannot be effectively inhibited: At present, most of the functional skin care products of the emotion type focus on the regulation of the nervous system pathway, that is, the connection between the nervous system and the downstream target cells or effectors is blocked or inhibited to achieve the effect of improving stress and relieving tension. However, this solution lacks multi-pathway regulation, and the effect dimension is relatively single, so that the multi-aspect improvement effect of this type of skin care product is limited.

[0006] (3) The efficacy components are unstable, and the penetration and utilization efficiency is not high. Some skin care products rely on natural active components, such as phenolic substances in plant natural extracts, to achieve the regulation and improvement effect. If these components are not treated by special process, they are easy to be inactivated when exposed to air, resulting in a shorter action time. In addition, some components have relatively large molecular mass, and are difficult to penetrate the skin barrier, so that the absorption and utilization efficiency of cells is not high, and the comprehensive improvement effect is not obvious. SUMMARY

[0007] The purpose of the present application is to overcome the shortcomings of the prior art and provide a molecular-neuro dual-pathway synergistic targeted barrier repair composition and its application, forming a dual-pathway improvement approach of molecular regulation and sensory experience, and having the effects of blocking stress signal transmission, accelerating skin physiological repair, and maintaining positive emotions.

[0008] The purpose of the present application can be achieved by the following technical solution: a dual-pathway skin emotion regulation composition, comprising an efficacy component composition of saffron extract, ginseng extract, and tuberose extract at a mass ratio of (0.01-1) : (0.01-1.5) : (0.01-0.5).

[0009] Further, the mass ratio of the saffron extract, ginseng extract, and tuberose extract is (0.1-0.75) : (0.1-1) : (0.1-0.3).

[0010] The saffron extract and ginseng extract are wrapped in liposomes, and the mass ratio of the saffron extract liposome, ginseng extract liposome, and tuberose extract in the efficacy component composition is (0.01-3) : (0.01-3) : (0.01-0.5). Further, the mass ratio of the saffron extract liposome, ginseng extract liposome, and tuberose extract is (0.2-3) : (0.2-3) : (0.1-0.3). Further, the mass ratio of the saffron extract liposome, ginseng extract liposome, and tuberose extract is (0.2-1.5) : (0.2-1.5) : (0.1-0.2). Further, the mass ratio of the saffron extract liposome, ginseng extract liposome, and tuberose extract is (0.2-1.5) : (0.2-1.5) : (0.1-0.2).

[0011] Further, the saffron extract liposome is obtained by the following steps: (1) Oil phase preparation: soybean lecithin, cholesterol are weighed and added into anhydrous ethanol, 45-55℃ water bath, magnetic stirring until completely dissolved; (2) Thin film preparation: the mixture obtained in step (1) is evaporated under reduced pressure at 30-40℃, 0.05-0.1MPa to completely remove ethanol until a uniform lipid film is formed on the inner wall of the reactor; (3) Water phase preparation: saffron extract is weighed and added into phosphate buffer, 35-40℃ water bath, magnetic stirring until completely dissolved; (4) Liposome hydration and encapsulation: the water phase obtained in step (3) is slowly added to the lipid film obtained in step (2), 35-40℃ water bath, stirring for 0.5-1.5h to form a preliminary emulsion; (5) Particle size optimization: the preliminary emulsion is transferred to an ultrasonic cell disruptor, and the probe ultrasonic method is used to reduce the particle size of the liposome under ice water bath conditions until the suspension is translucent; (6) Purification of liposome: the mixed solution after ultrasonic treatment is filtered through a 0.45μm microporous filter membrane to remove large particles.

[0012] Further, the mass ratio of soybean lecithin, cholesterol and saffron extract is (8-12):(1-3):(0.5-1.5); In step (5), the power of the ultrasonic cell disruptor is 250-350W, the pulse mode is working for 2-4s and pausing for 2-4s, and the total time is 10-20min.

[0013] Further, the ginseng extract liposome is obtained by the following steps: (1) Oil phase preparation: soybean lecithin, cholesterol are weighed and added into anhydrous ethanol, 45-55℃ water bath, magnetic stirring until completely dissolved; (2) Water phase preparation: ginseng extract is weighed and added into phosphate buffer, 35-40℃ water bath, magnetic stirring until completely dissolved; (3) Liposome hydration and encapsulation: the water phase obtained in step (2) is slowly added to the oil phase obtained in step (1), and ice bath ultrasonic emulsification is carried out at a power of 200-300W for 5-15min to form a preliminary emulsion; (4) Solvent removal: the preliminary emulsion is placed in a rotary evaporator and evaporated under reduced pressure at 40-50℃, vacuum degree 0.05-0.1MPa, time 40-60min to completely remove the ether, and the evaporation process is stirred every 8-12min for 1-2min; (5) high pressure homogenization: adding phosphate buffer to the mixture obtained in step (4), stirring for 25-35 min to make the mixture uniformly distributed in the solvent, and then treating with a high pressure homogenizer, the pressure being 60-120 MPa, and the cycle being 2-5 times, to obtain the safflower extract liposome with uniform particle size.

[0014] Further, the mass ratio of the soybean lecithin, cholesterol, vitamin E and ginseng extract is (10-20):(2-3):(0.05-0.15):(1-2). The pH of the phosphate buffer is 6-7.

[0015] The application further provides a use of the crocus extract or the liposome thereof, the ginseng extract or the liposome thereof and the tuberose extract as an efficacy component composition in the preparation of a skin care product. The skin care product can be any form of skin care product or cosmetic product, including skin care water, essence, emulsion, cream, etc. The required base components are different according to the different types of skin care products. The skin care product has the effects of fresh fragrance, soothing and relaxing, stress resistance repair and improvement of skin condition.

[0016] Further, the skin care product is preferably an essence, which comprises the following components by weight: A phase: deionized water to 100 glycerol 3.0-7.0; 1,3-butanediol 1.0-3.0; sodium hyaluronate 0.3-0.8; carbomer 0.1-0.5; xanthan gum 0.01-0.5; EDTA-disodium 0.01-0.5; B phase: tetrahydro piperonyl 0.1-1; hydrogenated lecithin 0.1-1; caprylic / capric acid triglyceride 0.1-3; vitamin E 0.01-1; C phase: citric acid buffer 0.01-1; D phase: efficacy component composition 0.03-6.5; E phase: phenoxyethanol 0.1-2; 1,2-hexanediol 0.1-3; fragrance 0.1-1.

[0017] The essence is prepared by the following method: Water phase preparation: take a certain amount of deionized water in the reactor, then weigh the glycerol, 1,3 butanediol, sodium hyaluronate, carbomer, xanthan gum, EDTA-2 sodium according to the formula proportion, slowly add into the reactor, 70~80℃ water bath heating, constant stirring, make each component fully dissolved; Oil phase preparation: weigh the tetrahydropeperidine, hydrogenated lecithin, caprylic acid / capric acid triglyceride according to the formula proportion in the reactor, 60~70℃ water bath heating, stirring and mixing. When the temperature of the mixture drops to 30~40℃, add the corresponding mass of vitamin E and the efficacy ingredient composition (i.e. saffron extract or its liposome, ginseng extract or its liposome, tuberose extract), constantly stirring, until a transparent and uniform system is formed; Water phase-oil phase mixing: slowly pour the prepared oil phase into the cooled water phase reactor, stirring while pouring, speed 800-1000rpm, for 30 minutes, so that the system is emulsified uniformly (no stratification, no oil droplets); (4) Add phenoxyethanol, 1,2-hexanediol and essence to the mixture, and adjust the pH to 5.5~6.5 with citric acid buffer, and finally homogenize to obtain the essence.

[0018] Compared with the prior art, the present application has the following beneficial effects: (1) The saffron extract contains rich crocin and saffronic acid, which can inhibit the activity of the key enzyme (11β-hydroxysteroid dehydrogenase type 1, 11β-HSD1) in the synthesis of cortisol, directly reduce the level of stress hormone in the skin, fundamentally reduce the irritation and damage of excessive secretion of stress factor cortisol to the skin, has the effect of reducing stress and relaxing, improves the skin microcirculation and relieves the dark and dull skin color. The present application takes saffron extract and ginseng extract as the core components, and preferably encapsulates them in the form of liposomes. This method can not only ensure the persistent activity of the components, but also improve the penetration rate and absorption utilization rate of the components, so that the skin care product has the effects of stress repair and improvement of skin condition.

[0019] (2) The ginseng extract contains a large amount of ginsenosides, which has the effects of repairing barrier and metabolic regulation. The ginseng extract can activate the proliferation of fibroblasts and keratinocytes, promote the synthesis of collagen and barrier proteins, on the one hand, it can improve the skin condition and enhance the skin elasticity, on the other hand, it can repair the skin barrier, reduce the transdermal water loss, and improve the skin tolerance. In addition, ginsenosides can also promote the secretion of β-endorphin by peripheral tissues (such as skin) and pituitary, which can not only inhibit the release of inflammatory signals and has the effect of analgesia and relaxation, but also can make the brain produce a pleasant feeling, relieve anxiety and stress.

[0020] (3) The tuberose extract contains rich volatile aromatic components, and the volatile aromatic substances in the tuberose extract can transmit signals to the brain through olfactory receptors in the nasal cavity, so that the brain produces a comfortable and relaxed feeling, reduces subjective anxiety, awakens positive emotions, and further inhibits the neural transmission of stress signals.

[0021] (4) The present application breaks through the single pathway regulation mode and constructs a skin and emotion regulation system mainly composed of saffron extract, ginseng extract and tuberose extract. The saffron extract can inhibit the activity of the key enzyme (11β-hydroxysteroid dehydrogenase type 1, 11β-HSD1) in cortisol synthesis, thereby fundamentally reducing the effect of endogenous stress hormones on skin cells. The ginseng extract can activate the expression of skin barrier protein genes and repair the skin state; at the same time, the ginsenosides in the ginseng extract can promote the secretion of β-endorphin, so that the skin is relaxed and a pleasant feeling is produced. The tuberose extract contains various volatile aromatic substances, which can transmit signals to the brain through olfactory receptors, produce a comfortable and relaxed feeling, and reduce subjective anxiety. In addition, the saffron extract and the ginseng extract are wrapped in the form of liposomes, which can greatly improve the penetration efficiency and the degree of cell absorption and utilization. These components are used in synergy to form a double-pathway improvement approach of molecular regulation and sensory experience, which has the effects of blocking the transmission of stress signals, accelerating the physiological repair of the skin, and maintaining positive emotions. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 The changes in the skin redness degree of the facial skin of the subject before and after 15 minutes, 14 days and 28 days of using the essence of Example 1 are shown in Table 1. DETAILED DESCRIPTION

[0023] The present application will be described in detail below in combination with the drawings and specific examples. The present embodiment is implemented on the premise of the technical solution of the present application, and detailed implementation modes and specific operation processes are given, but the protection scope of the present application is not limited to the following examples.

[0024] The various raw materials used in the present application are commercially available in the art, and the equipment used is conventional in the art.

[0025] The present application constructs a skin and emotion regulation system mainly composed of saffron extract, ginseng extract and tuberose extract, wherein: Cortisol is a glucocorticoid secreted by the adrenal cortex, often referred to as the "stress hormone." In the short term, it has positive effects such as raising blood sugar and reducing inflammation during stress. However, long-term excessive intake can cause systemic damage, such as inhibiting collagen synthesis, accelerating elastin degradation, and inducing skin inflammation. Saffron extract is rich in crocin and crocinic acid, which can inhibit the activity of a key enzyme in cortisol synthesis (11β-hydroxysteroid dehydrogenase type 1, 11β-HSD1), directly reducing the level of stress hormones in the skin. This fundamentally alleviates the irritation and damage to the skin caused by excessive cortisol secretion, thus reducing stress and promoting relaxation, improving skin microcirculation, and alleviating dull skin tone. The saffron extract used is a conventional commercially available product in this field.

[0026] Saffron extract can also be encapsulated in liposomes, which are obtained through the following steps: (1) Oil phase preparation: Weigh soybean lecithin and cholesterol, add them to anhydrous ethanol, and stir magnetically in a water bath at 45~55℃ until completely dissolved. The mass-volume ratio of soybean lecithin to anhydrous ethanol is 1:(15~25) g / mL, and the amount of cholesterol added is calculated according to the mass ratio of soybean lecithin to cholesterol as (8~12):(1~3); (2) Thin film preparation: Transfer the mixture obtained in step (1) to a rotary evaporator and evaporate under reduced pressure at 30~40℃ (vacuum degree 0.05-0.1MPa) to completely remove ethanol until a uniform lipid film is formed on the inner wall of the flask.

[0027] (3) Aqueous phase preparation: Weigh saffron extract and add it to phosphate buffer (PBS, pH 7.0). Heat in a water bath at 35-40°C and stir magnetically until completely dissolved. The mass-volume ratio of saffron extract to phosphate buffer is 1:(80-120) g / mL. The amount of saffron extract added is calculated according to the mass ratio of soybean lecithin to saffron extract as (8-12):(0.5-1.5). (4) Liposome hydration and encapsulation: The aqueous phase obtained in step (3) is slowly added to the lipid film obtained in step (2), and the mixture is heated in a water bath at 35~40℃ and magnetically stirred for 0.5~1.5h to form a primary emulsion.

[0028] (5) Particle size optimization: Transfer the colostrum to an ultrasonic cell disruptor and reduce the size of liposomes by ultrasonic probe under ice-water bath conditions. Power 250~350W, pulse mode: work for 2~4s, pause for 2~4s, total time 10~20min until the suspension is semi-transparent.

[0029] (6) Purification of liposomes: The ultrasonically treated mixed solution was filtered through a 0.45μm microporous membrane to remove large particles.

[0030] Ginseng extract is a commercially available product. It contains a large amount of ginsenosides, which have barrier-repairing and metabolic-regulating effects. Ginseng extract can activate the proliferation of fibroblasts and keratinocytes, and promote the synthesis of collagen and barrier proteins. On the one hand, it can improve skin condition and enhance skin elasticity; on the other hand, it can repair the skin barrier, reduce transepidermal water loss, and improve skin tolerance. Furthermore, ginsenosides can promote the secretion of β-endorphins from peripheral tissues and the pituitary gland. This can inhibit the release of inflammatory signals, have analgesic and relaxing effects, and also induce feelings of pleasure in the brain, relieving anxiety and stress.

[0031] Ginseng extract can also be encapsulated in liposomes, which are obtained using the following steps: (1) Preparation of oil phase: Weigh soybean lecithin and cholesterol, add them to ether, then weigh vitamin E into ether, and stir magnetically in a water bath at 35~45℃ until completely dissolved; the mass-volume ratio of soybean lecithin to ether is (1~2):(15~25) g / mL, and the amount of each component added is calculated according to the mass ratio of soybean lecithin:cholesterol:vitamin E as (10~20):(2~3):(0.05~0.15); (2) Aqueous phase preparation: Weigh the ginseng extract and add it to the phosphate buffer solution. Heat it in a water bath at 35~40℃ and stir magnetically until it is completely dissolved. The mass-volume ratio of ginseng extract to phosphate buffer solution is (1~2):(30~60) g / mL. The amount of ginseng extract added is calculated according to the mass ratio of ginseng extract to soybean lecithin (1~2):(10~20). (3) Liposome hydration and encapsulation: The aqueous phase obtained in step (2) is slowly added to the oil phase obtained in step (1), and at the same time, the mixture is ultrasonically emulsified in an ice bath at a power of 200~300W for 5~15 minutes to form a primary emulsion. (4) Solvent removal: Place the primary emulsion in a rotary evaporator and evaporate under reduced pressure at 40~50℃ with a vacuum of 0.05~0.1MPa for 40-60 minutes to completely remove the ether. Stir once every 8~12 minutes during the evaporation process, and stir for 1~2 minutes each time. (5) High pressure homogenization: Add phosphate buffer to the mixture obtained in step (4), stir magnetically for 25-35 minutes to make the mixture evenly distributed in the solvent, and then process it with a high pressure homogenizer at a pressure of 60-120 MPa for 2-5 cycles to obtain ginseng extract liposomes with uniform particle size.

[0032] Tuberose extract is a commercially available product. It contains abundant volatile aromatic compounds, which transmit signals to the brain via olfactory receptors in the nasal cavity, inducing feelings of comfort and relaxation, reducing subjective anxiety, evoking positive emotions, and thus inhibiting the neurotransmission of stress signals. These three components work synergistically: saffron extract inhibits the activity of a key enzyme in cortisol synthesis (11β-hydroxysteroid dehydrogenase type 1, 11β-HSD1), fundamentally reducing the damage to the skin caused by excessive cortisol secretion, thereby improving dull skin tone, reducing stress, and promoting relaxation. Ginseng extract activates the expression of skin barrier protein genes, repairs skin condition, and enhances skin tolerance; simultaneously, ginsenosides promote β-endorphin secretion, relaxing the skin and producing a pleasant feeling. The various volatile aromatic substances in tuberose extract transmit signals to the brain via olfactory receptors in the nasal cavity, inhibiting the neurotransmission of stress signals, producing feelings of comfort and relaxation, and reducing subjective anxiety. Furthermore, encapsulating saffron extract and ginseng extract in liposome form can ensure the long-lasting activity of the ingredients, as well as improve their permeability and absorption rate, thus achieving both molecular regulation and sensory experience, with significant advantages in both aspects.

[0033] The composition of this invention can be used in skincare products, including but not limited to toners, serums, lotions, and creams. In preparation, in addition to the above composition, the skincare products also include base components necessary for the production of various skincare products. These base components can be selected from conventional base components used in existing products, and the skincare products can be prepared using conventional methods from the prior art.

[0034] I. Experiment 1 To verify the core efficacy of the combination of saffron extract, ginseng extract, and tuberose extract, and to explore the effect of liposome encapsulation technology on enhancing efficacy, the following experiments were conducted: 1. Experimental Samples Prepare the experimental samples according to Table 1: Experimental Group 1 and Experimental Group 2, with saffron extract, ginseng extract and tuberose extract as active ingredients, mix the corresponding mass of the active ingredients: commercially available saffron extract, ginseng extract and tuberose extract powder directly with the base matrix to obtain experimental samples 1-2. Experimental Groups 3 and 4: Using saffron extract liposomes, ginseng extract liposomes, and tuberose extract as active ingredients, the active ingredients saffron extract and ginseng extract were first made into liposomes, and then mixed directly with tuberose extract and the base matrix to obtain experimental samples 3-4.

[0035] Table 1. Amount and Form of Experimental Active Ingredients in: 1) Saffron extract liposomes were prepared by the following method: (1) Oil phase preparation: Weigh soybean lecithin and cholesterol, add them to anhydrous ethanol (the mass-to-volume ratio of soybean lecithin to anhydrous ethanol is 1:20 g / mL), and stir magnetically in a 50℃ water bath until completely dissolved. The mass ratio of soybean lecithin, cholesterol, and saffron extract is 10:2:1.

[0036] (2) Thin film preparation: The mixture was transferred to a rotary evaporator and evaporated under reduced pressure at 35°C (vacuum degree 0.08MPa) to completely remove ethanol until a uniform lipid film was formed on the inner wall of the flask.

[0037] (3) Aqueous phase preparation: Weigh the saffron extract and add it to phosphate buffer (PBS, pH 7.0) (the mass-volume ratio of saffron extract to PBS is 1:100 g / mL). Heat in a 37°C water bath and stir magnetically until completely dissolved.

[0038] (4) Liposome hydration and encapsulation: The aqueous phase is slowly added to the lipid film, and the mixture is heated in a 37°C water bath and magnetically stirred for 1 hour to form a primary emulsion.

[0039] (5) Particle size optimization: The colostrum was transferred to an ultrasonic cell disruptor and the liposome particle size was reduced by probe ultrasound under ice-water bath conditions. The power was 300W, the pulse mode was 3s working and 3s pausing, and the total time was 15min until the suspension was semi-transparent.

[0040] (6) Purification of liposomes: The ultrasonically treated mixed solution was filtered through a 0.45μm microporous membrane to remove large particles, thus obtaining a saffron extract liposome suspension.

[0041] The suspension can be used directly by calculating the appropriate mass (based on the saffron extract it contains) or after concentration / dilution, depending on different needs.

[0042] 2) Ginseng extract liposomes were prepared by the following method: (1) Oil phase preparation: Weigh soybean lecithin and cholesterol, add them to diethyl ether (the mass-to-volume ratio of soybean lecithin to diethyl ether is 1.5:20 g / mL), then weigh vitamin E and add it to the mixture. Heat in a 40℃ water bath and stir magnetically until completely dissolved. The mass ratio of soybean lecithin, cholesterol, vitamin E, and ginseng extract is 15:2.5:0.08:1.5.

[0043] (2) Preparation of aqueous phase: Weigh the ginseng extract and add it to phosphate buffer (PBS, pH 6.5) (the mass-to-volume ratio of ginseng extract to PBS is 1.5:50 g / mL). Heat in a 37°C water bath and stir magnetically until completely dissolved. (3) Liposome hydration and encapsulation: Slowly add the aqueous phase to the oil phase and simultaneously sonicate in an ice bath (power 250W, 10min) to form a primary emulsion.

[0044] (4) Solvent removal: Place the primary emulsion in a rotary evaporator and evaporate under reduced pressure at 45°C (vacuum degree 0.09MPa) for 40-60 minutes to completely remove the ether. Stir once every 10 minutes during the evaporation process, and stir for 1 minute each time.

[0045] (5) High-pressure homogenization: Add phosphate buffer (volume ratio of 1:1 to the primary emulsion) to the above solvent-removed mixture, and stir magnetically for 30 min to ensure uniform distribution of the mixture in the solvent. Then process with a high-pressure homogenizer (pressure 100 MPa, cycle 3 times) to obtain ginseng extract liposome suspension.

[0046] The suspension can be used directly by calculating the appropriate mass (based on the ginseng extract it contains) or after concentration / dilution, depending on different needs.

[0047] 2. Experimental Methods and Results (1) In vitro 11β-HSD1 enzyme inhibition rate test Experimental subjects: In vitro molecular experiments were conducted using experimental groups 1-4 and the blank group, with the blank group serving as the control group. Reagents and instruments: ① Recombinant human 11β-HSD1 enzyme, NADPH (reduced nicotinamide adenine dinucleotide phosphate), cortisone (substrate), Tris-HCl buffer; ② Constant temperature shaking incubator (37℃), HPLC system (equipped with UV detector, detection wavelength: 245nm), ultra-high performance liquid chromatography column (C18 reversed phase column); Experimental Principle: 11β-HSD1 is an NADPH-dependent enzyme that, in the presence of NADPH, catalyzes the conversion of inactive cortisol into the active glucocorticoid cortisol. HPLC detection of cortisol levels reflects the inhibitory effect of the experimental composition on this conversion process.

[0048] Experimental steps: The enzyme-catalyzed reaction system (100 μL) is shown in the table below: Table 2. Amounts of enzyme-catalyzed reaction system ② Prepare a 100 μL reaction system according to the components and concentrations shown in Table 2. Add recombinant human 11β-HSD1 enzyme, NADPH, the experimental composition, and Tris-HCl buffer to the reaction tube sequentially. Finally, add the substrate cortisone to initiate the reaction. After vortexing, place the reaction system in a 37°C constant temperature shaking incubator and incubate for 10 minutes to allow the system temperature to equalize and the inhibitor to fully bind with the enzyme. Then continue the reaction for another 30 minutes.

[0049] ③ After the reaction is complete, immediately add 200 μL of ice-cold acetonitrile to each reaction tube to terminate the reaction. Vortex mix well, let stand at 4℃ for 5 minutes, and then centrifuge at 12000 rpm for 10 minutes at 4℃.

[0050] ④HPLC detection: Take the supernatant and use an HPLC system to detect the cortisol content. HPLC detection parameters and conditions are shown in Table 3. Table 3 HPLC detection parameters and conditions ⑤ Using the blank group as the control group, the inhibition rate of recombinant human 11β-HSD1 enzyme in experimental groups 1-4 was calculated. Inhibition rate (%) = (1 - cortisol level in experimental group / cortisol level in control group) * 100%. Experimental results are shown in Table 4 below. Table 4. Inhibition rate of 11β-HSD1 enzyme by experimental samples Table 4 shows that even a simple mixture of the three active ingredients exhibited inhibitory activity against the 11β-HSD1 enzyme, with inhibition rates of 10.86% and 12.12%, respectively, in a concentration-dependent manner. This indicates that saffron extract, ginseng extract, and tuberose extract can synergistically inhibit this key enzyme in cortisol synthesis even in their unencapsulated state. When the ingredients were encapsulated in liposomes, the inhibition rates increased to 14.47% and 16.05%, respectively, demonstrating that liposome technology significantly enhances the ability of the active ingredients to target and inhibit enzyme activity by improving their stability or bioavailability.

[0051] Preliminary evaluation of human sensory organs Test samples: Test samples 1-4 and blank group samples; Test subjects: Fifteen volunteers were recruited, all of whom had some emotional skin problems and all had sensitive skin. Before the test, they signed a written informed consent form and were then randomly divided into five groups, each using the same product.

[0052] Test Method: Subjects completed facial cleansing at the testing center and sat quietly for 30 minutes in a temperature and humidity controlled environment. They then underwent a blind test using the corresponding experimental sample to assess their immediate soothing sensation. The results are shown in Table 5 below. Table 5. Average scores of human sensory evaluation (0-5 points) As shown in Table 5, all samples containing active ingredients demonstrated superior sensory experience compared to the pre-control group. The liposome-encapsulated group achieved significantly higher scores on the two key indicators of "immediate cooling / soothing sensation" and "visual improvement in skin redness." Volunteers reported that the liposome-encapsulated product felt more moisturizing, had better penetration, and exhibited a faster and more significant soothing effect. This indicates that liposome technology not only enhances efficacy at the molecular level but also optimizes the product's sensory experience and immediate repair effects.

[0053] Preliminary experimental results show that even when saffron extract, ginseng extract, and tuberose extract are used in a simple mixture, they can achieve certain enzyme inhibition and sensory improvement effects through the synergistic effect of multiple components. However, liposome encapsulation technology can significantly improve various efficacy indicators, including enzyme inhibition rate, immediate soothing sensation, and skin appearance improvement.

[0054] II. Experiment II To verify the superior efficacy of the active ingredient combination containing saffron extract liposomes, ginseng extract liposomes, and tuberose extract, a serum was prepared using the active ingredient combination as an example. The content of each component in the serum is shown in Table 6 below: Table 6. Serum Formula Ratio Table To further illustrate the role and effect of each component in this invention, experiments were conducted through the following examples and comparative examples: Example 1 The composition consists of the following components: 0.2 parts of saffron extract liposomes, 0.2 parts of ginseng extract liposomes, and 0.1 parts of tuberose extract.

[0055] Example 2 The composition consists of the following components: 1.5 parts of saffron extract liposomes, 1.5 parts of ginseng extract liposomes, and 0.2 parts of tuberose extract.

[0056] Example 3 The composition consists of the following components: 3 parts saffron extract liposomes, 3 parts ginseng extract liposomes, and 0.3 parts tuberose extract.

[0057] Comparative Example 1 The composition consists of the following components: 0 parts of saffron extract liposomes, 0.3 parts of ginseng extract liposomes, and 0.2 parts of tuberose extract.

[0058] Comparative Example 2 The composition consists of the following components: 0.3 parts of saffron extract liposomes, 0 parts of ginseng extract liposomes, and 0.2 parts of tuberose extract.

[0059] Comparative Example 3 The composition consists of the following components: 0.25 parts of saffron extract liposomes, 0.25 parts of ginseng extract liposomes, and 0 parts of tuberose extract.

[0060] Comparative Example 4 The composition consists of the following components: 0 parts of saffron extract liposomes, 0 parts of ginseng extract liposomes, and 0.5 parts of tuberose extract.

[0061] Comparative Example 5 The composition consists of the following components: 0 parts of saffron extract liposomes, 0.5 parts of ginseng extract liposomes, and 0 parts of tuberose extract.

[0062] Comparative Example 6 The composition consists of the following components: 0.5 parts of saffron extract liposomes, 0 parts of ginseng extract liposomes, and 0 parts of tuberose extract.

[0063] Blank comparison example 7 The composition consists of the following components: 0 parts of saffron extract liposomes, 0 parts of ginseng extract liposomes, and 0 parts of tuberose extract.

[0064] The formulations of the compositions obtained in the above embodiments and comparative examples in the serum are shown in Table 7 below: Table 7. Formulation ratios of the serums in each embodiment and comparative example.

[0065] The preparation method of the serum is as follows: (1) Aqueous phase preparation: Take an appropriate amount of deionized water into the reactor, and then weigh glycerol, 1,3-butanediol, sodium hyaluronate, carbomer, xanthan gum, and disodium EDTA according to the formula ratio. Slowly add them into the reactor, heat in a water bath at 70-80℃, and stir continuously to ensure that each component is fully dissolved.

[0066] (2) Oil phase preparation: Tetrahydropiperine, hydrogenated lecithin, and caprylic / capric triglycerides were weighed into a reactor according to the formula ratio and heated in a water bath at 60-70℃, stirring until homogeneous. When the mixture temperature dropped to approximately 35℃, the corresponding masses of vitamin E, saffron extract liposomes, ginseng extract liposomes, and tuberose extract were added, and the mixture was stirred continuously until a transparent and homogeneous system was formed. The saffron extract liposomes and ginseng extract liposomes were prepared using the same method as in Experiment 1. (3) Aqueous-oil phase mixing: Slowly pour the prepared oil phase into the cooled aqueous reactor while stirring at 800-1000 rpm for 30 minutes to ensure uniform emulsification of the system (no layering, no oil droplets).

[0067] (4) Add phenoxyethanol, 1,2-hexanediol and fragrance to the mixture, adjust the pH to 5.5-6.5 with citrate buffer, and finally homogenize to obtain the essence.

[0068] Performance testing: I. In vitro molecular experiments Experimental subjects: In vitro molecular experiments were conducted using the efficacy component compositions of each example and comparative example. Specifically, the efficacy components of each example and comparative example—saffron extract liposomes, ginseng extract liposomes, and tuberose extract—were directly mixed, and the resulting mixtures were used as experimental subjects. Examples 1-3 and comparative examples 1-6 served as the experimental group, while blank comparative example 7 served as the control group. 11β-HSD1 enzyme inhibition rate experiment: The same method as the in vitro 11β-HSD1 enzyme inhibition rate test in Experiment 1 was used, wherein the experimental composition in the enzyme-catalyzed reaction system was the active ingredient composition of each example and comparative example in Table 5; Using blank control example 7 as the reference group, the inhibition rate of recombinant human 11β-HSD1 enzyme in Examples 1-3 and Comparative Examples 1-6 was calculated. Inhibition rate (%) = (1 - Cortisol level in the control group / Cortisol level in the experimental group) * 100% (4) Experimental results Based on the above experimental procedures, the inhibition rates of recombinant human 11β-HSD1 enzyme in each group were measured as shown in the table below: Table 8. Inhibition rate of recombinant human 11β-HSD1 enzyme As shown in Table 8, saffron extract liposomes, as the core active ingredient for inhibiting the enzyme activity, exhibited an inhibition rate of 15.23% when used alone in Comparative Example 6. In contrast, the inhibition rates of ginseng extract liposomes and tuberose extract, when used alone, were both below 1.5%, indicating weak direct inhibitory effects on the enzyme. However, when the three were combined in a specific ratio, a significant synergistic effect was observed. Although the amount of saffron extract liposomes used in Example 1 was only 0.2 parts, lower than the 0.5 parts used in Comparative Example 6, its inhibition rate reached 18.53%, indicating that the addition of ginseng extract liposomes and tuberose extract effectively enhanced the bioactivity of saffron extract. Furthermore, the inhibitory effect of Example 1 was significantly better than that of Comparative Example 2 (lacking ginseng extract liposomes) and Comparative Example 3 (lacking tuberose extract), with inhibition rates of 12.45% and 11.88%, respectively, demonstrating that the combined effect of the three constituted the optimal efficacy system. As the content of each component in the composition gradually increased from Example 1 to Example 3, the inhibition rate increased in a concentration-dependent manner, from 18.53% to 31.26% to 47.01%, further verifying the reliability and controllability of the composition in inhibiting cortisol production at the molecular level through a synergistic pathway.

[0069] II. Human Efficacy Evaluation Experiment (1) Test samples: the serums prepared from the components of Examples 1-3, Comparative Examples 1-6 and Blank Comparative Example 7 as described in Table 7; (2) Test subjects: 100 healthy individuals aged 25 to 60 years, all of whom had certain emotional skin problems and were all sensitive skin. Before the test, they signed a written informed consent form and were then randomly divided into 10 groups, with each group using the same product.

[0070] (3) Skin repair test method: ① Subjects completed facial cleansing at the testing center, sat quietly for 30 minutes in a constant temperature and humidity environment, and then used VISIA image acquisition and skin color meter to test skin redness, and used a non-invasive skin tester to test the transepidermal water loss (TEWL) value of the face.

[0071] ② Subsequently, each group member used the corresponding test serum. After 15 minutes, the skin redness was tested using the VISIA image sensor and skin tone analyzer, and the transepidermal water loss (TEWL) value of the face was tested using a non-invasive skin analyzer.

[0072] ③ In addition, after using the serum for 14 and 28 days, the skin redness was tested again using the VISIA image sensor and skin tone analyzer, and the transepidermal water loss (TEWL) value of the face was tested using a non-invasive skin analyzer.

[0073] (4) Emotional Relief Test Method: Subjects completed facial cleansing at the testing center, sat quietly in a constant temperature and humidity environment for 30 minutes, then applied 1.5 mL of serum to their entire face and massaged it thoroughly until absorbed. The sensory experience test was completed within 15 minutes. The test content is as follows: ① Smell - Aroma Experience: Aroma Intensity (1-5 points, 1 point is no aroma, 5 points is strong aroma), Aroma Comfort (1-5 points, 5 points is "relaxing" aroma).

[0074] ② Visual assessment of skin redness: Three professional assessors scored the skin redness using high-definition images (1-5 points, 5 points for significant improvement).

[0075] ③Sensory-emotional experience assessment: level of pleasure (1-5 points, 5 points is very pleasant), level of anxiety (1-5 points, 5 points is very anxious), level of tension (1-5 points, 5 points is very tense).

[0076] (5) Test instructions ①Test environment: Temperature 20~22℃, humidity 40~60%RH.

[0077] ② Skin tone meter tests can convert skin redness into a specific value, namely EI value, through optical principles and color models. The range is 0~100. The higher the EI value, the redder the facial skin.

[0078] ③ The transdermal water loss rate (TWEL) can be calculated by detecting the water vapor partial pressure difference at different heights on the skin surface using a sensor, combined with the diffusion law.

[0079] ④ Test results can be calculated using the following formula: Change rate = (Analysis value after product use - Analysis value before product use) ÷ Analysis value before product use × 100%. The average change rate for each group is taken. Sensory experience evaluation is based on the average score of each group's items; the change rate is not calculated for this evaluation.

[0080] (6) Test results ①Redness reduction effect Based on the above experimental steps, the skin redness of the subjects was tested at different times using VISIA image acquisition and skin color meter, and the specific numerical values ​​reflected the degree of facial redness. The data of each group were statistically calculated, and the following results were obtained: Table 9. Changes in facial redness in different groups of subjects at different time points. Using the blank control example 7 as a reference, when only one of the following ingredients was added (comparisons 4-6): saffron extract liposomes, ginseng extract liposomes, and tuberose extract, the differences in facial redness between the subjects and those without the ingredient on day 28 were 4.64%, 10.29%, and 12.40%, respectively. This indicates that saffron extract has the best effect in relieving stress-related skin redness, followed by ginseng extract, while tuberose extract has the weakest effect. This may be because saffron extract can fundamentally inhibit the synthesis of the stress hormone cortisol, reducing skin redness caused by tension, anxiety, and other emotions, while ginseng extract can inhibit inflammatory responses to some extent and reduce skin redness, but its effect in relieving stress-related skin redness is slightly weaker. Tuberose extract showed a slight improvement when used alone, possibly because its volatile aromatic substances have a cooling and calming effect, relieving emotional stress and slightly reducing stress-related skin redness. Compared with Example 1, when the composition lacked one of the following components (Saffron Extract Liposomes, Ginseng Extract Liposomes, and Tuberose Extract) (Comparative Examples 1-3), the differences in facial redness after 28 days of use compared to the unused state were 11.35%, 13.07%, and 15.28%, respectively, all lower than the 16.65% in Example 1. This again demonstrates that Saffron Extract has the best effect in relieving skin redness, while Tuberose Extract has the weakest. Although Comparative Examples 2-3 also contained Saffron Extract, their effects were still lower than those in Example 1, which contained all three active ingredients, proving that there is a synergistic effect among the three active ingredients of the present invention, and simultaneous use can better improve facial redness. Observation of Examples 1-3 shows that the effect of improving facial redness increases with the increase of composition concentration.

[0081] Figure 1 The graph shows the changes in facial redness in subjects before, 15 minutes after, 14 days after, and 28 days after using the serum of Example 1. As can be seen from the graph, the degree of facial redness showed a continuous improvement trend over time after using the serum of Example 1. Specifically, facial redness was reduced after 15 minutes of product use, corresponding to a 7.66% reduction in redness in Table 6; after 14 days of continuous use, the improvement effect was further enhanced, with a 12.05% reduction in redness; and by day 28, the skin redness was significantly improved, with a reduction of 16.65%. The graph clearly illustrates this gradual improvement process: before use, a noticeable red area was visible on the face; after 15 minutes of use, the red area shrank and the color lightened; after 14 days, the red area further faded; and after 28 days, the facial skin tone had become more even, with only slight redness remaining. Figure 1 The data in Table 6 corroborate each other, indicating that the composition of the present invention not only has an immediate effect of quickly soothing redness, but also achieves stable barrier repair and skin tone improvement through continuous use.

[0082] Barrier repair effect Based on the above experimental steps, the transepidermal water loss (TEWL) value of the face was measured on the subjects at different times using a non-invasive skin testing device. The data from each group were statistically calculated, and the following results were obtained: Table 10. Changes in transdermal water loss rate of subjects in different time groups. As shown in Table 10, the composition of this invention exhibits a significant and continuously improving effect in reducing transdermal water loss. Data shows that all example groups demonstrated an increasingly enhanced barrier repair capacity over time, with Example 3 showing the most superior barrier repair effect, achieving a 25.08% reduction in transdermal water loss after 28 days of use. Comparative analysis reveals that ginseng extract plays a crucial role in the barrier repair process. Specifically, Comparative Example 5, containing only ginseng extract, reduced transdermal water loss by 11.87% after 28 days of use, while Comparative Example 6, containing only saffron extract, and Comparative Example 4, containing only tuberose extract, only reduced it by 8.52% and 3.92%, respectively. This significant difference in effect fully demonstrates the key role of ginseng extract in repairing skin barrier function. Furthermore, experimental data indicate a synergistic effect among the three active ingredients. Although Example 1 contained only 0.2 parts ginseng extract, its barrier repair effect reached 17.73% after 28 days of use, significantly better than the 11.87% improvement achieved by Comparative Example 5, which contained 0.5 parts ginseng extract. This phenomenon indicates that saffron extract, by reducing skin damage caused by stress hormones, combined with tuberose extract's effect of relieving anxiety, creates a more favorable environment for the barrier repair function of ginseng extract, thus producing a significant synergistic effect. Furthermore, the performance of the examples at all test time points was significantly better than any single-ingredient or two-ingredient combination comparative example, fully demonstrating the completeness and reliability of the technical solution of this invention in skin barrier repair. With prolonged use, the barrier repair effect of each example showed a continuously increasing trend, exhibiting good time-dependent improvement characteristics.

[0083] ③ Emotional relief effect Based on the above experimental steps, the sensory experience of the subjects was evaluated, with scores ranging from 0 to 5. The data from each group were statistically calculated, and the following results were obtained: Table 11 Sensory experience of subjects in different groups at different times As shown in Table 11, the composition of the present invention exhibits significant advantages in the three dimensions of smell, vision and sensation.

[0084] In terms of olfactory experience, the aroma intensity (3.2-3.6) and comfort (4.1-4.2) of Examples 1-3 remained at ideal levels. In contrast, Comparative Example 3, lacking tuberose extract, showed a significant decrease in both scores (2.1, 2.4), demonstrating that tuberose extract is the main source of the product's pleasant aroma. Notably, the Example group containing all three ingredients outperformed Comparative Example 4 (3.9), which contained only tuberose extract, in terms of aroma comfort, demonstrating the synergistic effect of saffron and ginseng extracts on the aroma experience.

[0085] In terms of visual improvement, the skin redness scores of Examples 1-3 (2.0-1.7) were significantly better than those of the other groups. In particular, although Comparative Example 2 (lacking ginseng extract) and Control Example 6 (containing only saffron extract) both contained saffron extract, their improvement effects (2.7, 3.7) were far less than those of Example 1, indicating that ginseng extract played a key role in enhancing the redness-reducing effect of saffron. Meanwhile, the effect of Example 1 was also significantly better than that of Comparative Example 3 (2.5), which only lacked tuberose extract, demonstrating that the optimal visual improvement effect can only be achieved through the combined action of all three components.

[0086] In terms of emotional experience, the Example 1 group performed best in all three dimensions: pleasure (3.9-4.2), anxiety (1.2-1.1), and tension (1.2-1.0). Specifically, Example 1's mood-soothing effect comprehensively surpassed any single-component group or two-component combination. For example, Comparative Example 1, which lacked saffron extract, had a significantly higher anxiety score, indicating that despite containing ginseng and tuberose, the lack of saffron extract's inhibitory effect on stress hormones prevented the achievement of optimal mood-soothing.

[0087] The three components used in this invention exhibit a significant synergistic effect. Tuberose extract directly soothes emotions through the olfactory pathway, saffron extract inhibits the production of stress hormones at the molecular level, and ginseng extract simultaneously participates in barrier repair and promotes endorphin secretion. These three components, through multi-pathway and multi-target synergistic effects, together constitute a unique molecular-neural dual-pathway regulatory mechanism, thus demonstrating outstanding comprehensive effects across all dimensions of sensory experience.

[0088] As can be seen from Table 11 above, Example 1, which uses all three active ingredients of the present invention, has better effects on smell, sight, and sensation than Comparative Examples 1-3, which contain only two of the components, and Comparative Examples 4-6, which contain only one of the components, proving that the three active ingredients of the present invention have a synergistic effect.

Claims

1. A molecular-neural dual-pathway synergistic targeted barrier repair composition, characterized in that: The active ingredient composition includes saffron extract, ginseng extract, and tuberose extract in a mass ratio of (0.01-1):(0.01-1.5):(0.01-0.5).

2. The molecular-neural dual-pathway synergistic targeted barrier repair composition according to claim 1, characterized in that: The mass ratio of the saffron extract, ginseng extract, and tuberose extract is (0.1-0.75): (0.1-1): (0.1-0.3).

3. The molecular-neural dual-pathway synergistic targeted barrier repair composition according to claim 1, characterized in that: The saffron extract and ginseng extract are encapsulated in liposomes, and the mass ratio of the saffron extract liposomes, ginseng extract liposomes, and tuberose extract in the active ingredient composition is (0.01-3):(0.01-3):(0.01-0.5).

4. The molecular-neural dual-pathway synergistic targeted barrier repair composition according to claim 3, characterized in that: The mass ratio of saffron extract liposomes, ginseng extract liposomes, and tuberose extract is (0.2-3):(0.2-3):(0.1-0.3).

5. The molecular-neural dual-pathway synergistic targeted barrier repair composition according to claim 3, characterized in that: The saffron extract liposomes were obtained using the following steps: Oil phase preparation: Weigh soybean lecithin and cholesterol, add them to anhydrous ethanol, and stir magnetically in a water bath at 45~55℃ until completely dissolved; Thin film preparation: The mixture obtained in step (1) is evaporated under reduced pressure at 30~40℃ and 0.05-0.1MPa to completely remove ethanol until a uniform lipid film is formed on the inner wall of the reactor; Aqueous phase preparation: Weigh the saffron extract, add it to the phosphate buffer solution, and stir magnetically in a 35-40°C water bath until completely dissolved; Liposome hydration and encapsulation: The aqueous phase obtained in step (3) is slowly added to the lipid film obtained in step (2), and the mixture is stirred in a water bath at 35~40℃ for 0.5~1.5h to form a primary emulsion; Particle size optimization: The colostrum was transferred to an ultrasonic cell disruptor and the liposome particle size was reduced by probe sonication under ice-water bath conditions until the suspension was translucent; Purification of liposomes: The ultrasonically treated mixture was filtered through a 0.45μm microporous membrane to remove large particles.

6. The molecular-neural dual-pathway synergistic targeted barrier repair composition according to claim 3, characterized in that: The ginseng extract liposomes were obtained using the following steps: (1) Preparation of oil phase: Weigh soybean lecithin and cholesterol, add them to ether, then weigh vitamin E into ether, and stir magnetically in a water bath at 35~45℃ until completely dissolved; (2) Aqueous phase preparation: Weigh the ginseng extract, add it to the phosphate buffer solution, and stir magnetically in a water bath at 35~40℃ until completely dissolved; (3) Liposome hydration and encapsulation: The aqueous phase obtained in step (2) is slowly added to the oil phase obtained in step (1), and at the same time, the mixture is ultrasonically emulsified in an ice bath at a power of 200~300W for 5~15 minutes to form a primary emulsion. (4) Solvent removal: Place the primary emulsion in a rotary evaporator and evaporate under reduced pressure at 40~50℃ with a vacuum of 0.05~0.1MPa for 40-60 minutes to completely remove the ether. Stir once every 8~12 minutes during the evaporation process, and stir for 1~2 minutes each time. (5) High pressure homogenization: Add phosphate buffer to the mixture obtained in step (4), stir for 25-35 minutes to make the mixture evenly distributed in the solvent, and then process it with a high pressure homogenizer at a pressure of 60-120 MPa for 2-5 cycles to obtain ginseng extract liposomes with uniform particle size.

7. The application of a molecular-neural dual-pathway synergistic targeted barrier repair composition as described in any one of claims 1 to 6, characterized in that: The aforementioned active ingredient composition can be used in skincare or cosmetic products.

8. The application of the molecular-neural dual-pathway synergistic targeted barrier repair composition according to claim 7, characterized in that: The skincare products mentioned include toners, serums, lotions, or creams.

9. The application of the molecular-neural dual-pathway synergistic targeted barrier repair composition according to claim 8, characterized in that: The skincare product is a serum, which comprises the following components by weight: Phase A: Deionized water to 100 Glycerin 3.0~7.0; 1,3-Butanediol 1.0~3.0; Sodium hyaluronate 0.3~0.8; Carbomer 0.1~0.5; Xanthan gum 0.01~0.5; EDTA-disodium 0.01~0.5; Phase B: Tetrahydropiperine 0.1~1; Hydrogenated lecithin 0.1~1; Caprylic / capric triglycerides 0.1~3; Vitamin E 0.01~1; Phase C: Citrate buffer solution 0.01~1; Phase D: 0.03-6.5g of the active ingredient composition; Phase E: Phenoxyethanol 0.1~2; 1,2-Hexanediol 0.1~3; Fragrance 0.1~1.

10. The application of the molecular-neural dual-pathway synergistic targeted barrier repair composition according to claim 6, characterized in that: The essence is prepared as follows: Aqueous phase preparation: Take an appropriate amount of deionized water into the reactor, and then weigh glycerol, 1,3-butanediol, sodium hyaluronate, carbomer, xanthan gum, and disodium EDTA according to the formula ratio. Slowly add them into the reactor, heat in a water bath at 70~80℃, and stir constantly to ensure that all components are fully dissolved. Oil phase preparation: Weigh tetrahydropiperine, hydrogenated lecithin, and caprylic / capric triglycerides into a reactor according to the formula ratio, heat in a water bath at 60-70°C, and stir until homogeneous. When the temperature of the mixture drops to 30-40°C, add the appropriate mass of vitamin E and the active ingredient combination, and stir continuously until a transparent and homogeneous system is formed; Aqueous-oil phase mixing: Slowly pour the prepared oil phase into the cooled aqueous reactor while stirring at 800-1000 rpm for 30 minutes to ensure uniform emulsification of the system. (4) Add phenoxyethanol, 1,2-hexanediol and fragrance to the mixture, and adjust the pH to 5.5-6.5 with citrate buffer. Finally, homogenize to obtain the essence.

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