Cultivation method for improving fruit setting of sesbania cannabina in northern area

Abstract

The invention discloses a cultivation method for improving fruit setting of stachys sieboldii in northern areas, and belongs to the technical field of plant cultivation. In order to solve the technical problem of difficult fruiting of sesbania cannabina due to low temperature in northern areas, the invention provides a set of complete cultivation technology system which comprises the key steps of mechanical seed skin breaking and constant-temperature germination accelerating, standardized seedling raising, greenhouse soil preparation, directional transplanting, reproductive period pruning, temperature and light management and the like. According to the method, natural short-day conditions in autumn and winter in the north are fully utilized, and through accurate temperature control, illumination control and nutrition regulation and control, the adverse effect of low temperature on sturdy sturdy sturdy sturdy sturdy sturdy sturdy sturdy sturdy sturdy sturdy sturdy sturdy sturdy sturdy sturdy sturdy Plant hormones are not used in the whole process, operation is easy and convenient, cost is low, the method is especially suitable for scientific research breeding and small-scale production of the sesbania cannabina, and effective technical support is provided for expanding the planting range of the sesbania cannabina and accelerating the breeding process.

Description

Technical Field

[0001] This invention belongs to the field of plant cultivation technology, and more specifically, relates to a cultivation method for improving the fruit setting of sesquiterpene in northern regions. Background Technology

[0002] Sesbania, belonging to the genus Sesbania in the subfamily Papilionoideae of the legume family, is mainly distributed in tropical and subtropical regions. As a plant with significant economic value, used for both fertilizer and forage, sesbania has soft leaves with a large biomass. It also exhibits tolerance to poor soil conditions and the ability to fix nitrogen through nodulation, making it a high-quality green manure that improves soil structure and enhances soil fertility. Its leaves are high in protein, rich in nutrients, and easily digestible, making them an excellent source of livestock feed. Sesbania seeds contain sesbania gum, a polysaccharide that can be used in oil drilling to improve recovery rates and as an additive in the paper, textile, and food industries.

[0003] In recent years, with increasing attention and development of marginal lands such as saline-alkali land, the strategy of "planting suitable crops for suitable land" for saline-alkali land improvement has gained increasing importance. Sesbania, due to its outstanding salt and alkali tolerance, large biomass, and efficient nodulation and nitrogen fixation capabilities, has become an ideal pioneer plant species for saline-alkali land improvement. Among plants in the genus Sesbania, Sesbania spinosa (… Sesbania bispinosa As a diploid species, its genome structure is simpler than that of the allotetraploid common sesquiterpene, making it suitable as a foundational genetic material for sesquiterpene research. Furthermore, the spiny sesquiterpene variety exhibits stronger salt and alkali tolerance; the relevant molecular mechanisms have been reported in the literature and possess significant scientific research value.

[0004] However, the growth and development of sesquiterpene are strictly limited by environmental conditions. Studies have shown that the flowering of sesquiterpene is regulated by photoperiod, exhibiting flowering under short-day conditions and inhibited by long-day conditions. Simultaneously, sesquiterpene has strict temperature requirements; it needs a warm environment for growth, and is susceptible to cold stress when temperatures drop below 5℃, leading to stunted growth, fruit set failure, and even death. Although the autumn and winter seasons in northern my country meet the short-day conditions, the generally low temperatures cannot simultaneously satisfy the photoperiod and temperature requirements for sesquiterpene fruit set, making it difficult for sesquiterpene to naturally set fruit in northern regions. Currently, patents such as CN117678490A only cover field cultivation techniques for common sesquiterpene and do not propose effective seed production solutions for northern regions specifically addressing the unique physiological characteristics of sesquiterpene. Existing technologies, such as conventional methods relying on plant hormone treatment or artificial lighting systems to regulate flowering, are not only costly but also interfere with the natural physiological processes of sesquiterpene, affecting seed genetic purity and making them unsuitable for research and breeding needs.

[0005] Therefore, developing a northern seed production technology for sesquiterpene that does not require exogenous hormones, does not rely on artificial lighting, and can effectively overcome low-temperature barriers is of great significance for expanding the planting range of sesquiterpene and accelerating the breeding process. Summary of the Invention

[0006] The purpose of this invention is to provide a cultivation method that improves the fruit setting of sesquiterpene in northern regions. By utilizing the natural short-day conditions in northern winters, the effective light duration is precisely controlled by the timed opening and closing of the insulating cotton quilt, and the low-temperature problem is solved by the double-layer greenhouse structure. This achieves a precise match between the photoperiod and temperature requirements of sesquiterpene, without the need for exogenous hormones or artificial lighting, thus solving the fruit setting obstacle caused by low temperature.

[0007] To achieve the above-mentioned objectives, the present invention adopts the following technical solution: This invention provides a cultivation method for improving the fruit setting rate of sesquiterpenes in northern regions, wherein the sesquiterpenes is... Sesbania bispinosa The method includes the following steps: S1. Seed breaking and germination: The seeds of Sesbania sesame are mechanically broken and then placed in a humid environment at a constant temperature of 37°C to germinate. S2. Seedling Management: Plant the pre-germinated seeds in a seedling substrate made of flower nutrient soil and vermiculite in a 1:1 volume ratio, and use 50-cell seedling trays for seedling cultivation; spray with Liminong powder once during the seedling period and manage ventilation. S3. Greenhouse land preparation: In northern regions, ridges are made inside greenhouses, and the ridges are covered with black plastic film. S4. Transplanting and planting: Transplant the seedlings with their original soil to the sunny side of the ridge, maintaining the plant spacing. S5. Reproductive regulation: When sesame enters the budding stage, prune the flower buds that have formed flower buds; spray Lime Nong powder during the flowering period, and maintain the minimum nighttime temperature no lower than 5℃ through temperature control measures, and control the effective light duration through light regulation measures. S6. Harvesting: Manage the seeds until they mature and then harvest. The main component of the Limefenone powder is superphosphate.

[0008] Furthermore, in step S1, the mechanical peeling process is performed using a rice milling machine for a short period of 15 seconds.

[0009] Furthermore, in step S2, the 50-cell seedling tray is made of PVC material, with a single-cell capacity of 50-60 ml.

[0010] Furthermore, in step S3, the width of the ridge is 50-60 cm, the height of the ridge is 15-25 cm, and the width of the furrow between the ridges is 20-30 cm.

[0011] Furthermore, in step S4, the plant spacing is 50 centimeters.

[0012] Furthermore, in step S5, the pruning treatment involves removing newly emerging lateral branches and compound leaves around the flower buds.

[0013] Furthermore, in step S5, the temperature control measures include using a double-layered structure for insulation, with the outer layer being a greenhouse and the inner layer having a detachable small arched shed. At night, the double-layered structure is used to maintain a minimum temperature of no less than 5°C.

[0014] Furthermore, in step S5, the light control measures include turning on the thermal insulation blanket from 8:30 to 16:00 every day to control the duration of light exposure.

[0015] Furthermore, in steps S2 and S5, Liminong powder is used for fertilization of seedlings and plants and for pest and disease control. Beneficial effects

[0016] This invention optimizes nutrient distribution through targeted pruning during the budding stage, significantly improving the seed setting rate of sesbania. It utilizes a double-layer greenhouse structure (outer greenhouse + inner small arched greenhouse) for effective insulation, while precisely controlling the effective light duration by opening and closing the insulating blankets from 8:30 to 16:00, creating a short-day environment suitable for sesbania. This invention accurately distinguishes between temperature and photoperiod control techniques, forming a synergistic system of double-layer insulation and light control. This satisfies the flowering requirements of sesbania while effectively overcoming the low-temperature barrier in northern regions. The entire process is conducted without the use of plant hormones or artificial lighting, reducing production costs and ensuring the purity of the seed's genetic background. All technical aspects form an organic whole, are easy to operate, and are suitable for scientific research, breeding, and large-scale production. Attached Figure Description

[0017] Figure 1 This is a picture of a sessile plant that has undergone pruning.

[0018] Figure 2 This is a picture of a sessilized plant that has not been pruned.

[0019] Figure 3 This is a comparison image of sesbania plants that have undergone pruning treatment versus those that have not.

[0020] Figure 4 This is a picture showing the fruit set of sesbania after it has been grown in a greenhouse in Beijing. Detailed Implementation

[0021] To enable those skilled in the art to better understand the technical solutions of this invention, the present application will be further described in detail below with reference to embodiments. Example 1

[0022] A cultivation method to improve the fruit setting of sesquiterpene in northern regions, the cultivation method mainly includes sesquiterpene seed germination, sesquiterpene seedling cultivation, selection of greenhouse planting soil, land preparation, transplanting and field management, pruning to improve sesquiterpene fruit setting, and post-cultivation harvesting, specifically including the following steps: S1. Germination of Sesbania seeds: Take 4 grams of Sesbania seeds, about 400 seeds, and use a rice milling machine to break the seed coat for 15 seconds. Place the broken Sesbania seeds in a petri dish lined with absorbent filter paper, add 10-15 ml of water, and place in a 37℃ incubator for germination for 24 hours; S2. Sesbania seedling cultivation: Plant the sesbania seeds that have been germinated in step S1 into the seedling substrate at a depth of 1.5 cm. Place them on the greenhouse seedbed for 30 days of seedling cultivation. Two weeks after the start of seedling cultivation, spray with Limeinong powder (main component is superphosphate) to fertilize the seedlings and prevent diseases and pests. Water every 3 days during the seedling cultivation period. During the seedling cultivation period, the seedling greenhouse should be semi-closed and ventilated. The seedling substrate includes the following raw materials by volume: 1 part flower nutrient soil, 1 part vermiculite; the seedling trays used for seedling cultivation are 50-cell seedling trays with a size of 540×280 mm, an upper diameter of 48×48 mm, a lower diameter of 23×23 mm, a height of 50 mm, a single cell capacity of 55 ml, and are made of PVC. S3. Selecting greenhouse planting soil: Select a moist, fertile, and humus-rich plot as the planting greenhouse. The soil in the planting greenhouse should be loose and have good water retention. After clearing away weeds, tree stumps and stones, and letting it dry for 5-10 days, use a deep tillage machine to plow, break up, rake and level the soil. S4. Land preparation: First, make ridges inside the greenhouse, with a ridge width of 50-60 cm, a ridge height of 15-25 cm, and a furrow width of 20-30 cm between ridges; cover the ridges with black plastic mulch. S5. Transplanting and Field Management: Transplant the sesbania seedlings that have completed the seedling cultivation in S2: Transplant the sesbania seedlings with their original soil to the planting strip in the greenhouse. Irrigate after transplanting, and water once a week during the subsequent plant cultivation period; plant the sesbania seedlings on the sunny side of the ridge with a spacing of 50 cm between seedlings. Spray with Lime Nong powder once two weeks after transplanting, and apply plant amino acid liquid fertilizer once one month after transplanting; S6. Pruning to Improve Sesbania Fruit Set: After transplanting, observe the flowering of Sesbania. When the planted Sesbania begins to bud, treat it by removing the new lateral branches and leaves near the flower buds that have already formed flower buds. After pruning, the Sesbania will... Figure 1 As shown, unpruned sesame seeds... Figure 2 As shown, the effects of pruning are comparable. Figure 3As shown, after pruning, the sesame plants are taller and have fewer small branches, which is beneficial for fruit setting. Two weeks after flowering, apply Limeng powder to fertilize the sesame plants and prevent pests and diseases. Implement a double-layer covering structure for the greenhouse: the outer layer is the main greenhouse structure, and the inner layer is a small arched shed with a height of 1.2m as the main heat preservation measure. The temperature is maintained at night by relying on the double-layer structure. Taking advantage of the natural short-day environment in northern winters, the heat preservation quilt is opened after 8:30 am and covered again after 4:00 pm to ensure that the minimum nighttime temperature does not fall below 5 degrees Celsius, which is conducive to the survival of sesame plants and the ripening of pods. Finally, plant and harvest sesame seeds according to conventional methods.

[0023] According to the above embodiments, the planting method described in this invention allows for the selection of strong sesbania seedlings for planting through early seedling cultivation and transplanting, thereby improving the survival rate of sesbania. Applying plant amino acid fertilizer in the early stages provides the necessary nutrients for sesbania growth. Using mulch effectively reduces weed growth, easing the workload of weeding and facilitating field management. Using Limeinong powder can prevent outbreaks of pests such as greenhouse spider mites and promote flowering and fruiting of sesbania. Northern regions have lower temperatures and fewer native pests and diseases, resulting in fewer pest and disease occurrences compared to propagation in southern regions. In greenhouses in Beijing, planting using existing methods yields sesbania with good fruit set. Figure 4 As shown.

[0024] Therefore, the planting method described in this invention can ensure the survival and fruit setting of sesquiterpene in northern regions, achieving the goal of propagating sesquiterpene in these areas. It reduces the difficulties in propagating sesquiterpene, promotes fruit setting, and meets the seed needs of sesquiterpene research.

Claims

1. A cultivation method for improving the fruit setting rate of sesquiterpene in northern regions, characterized in that, The sesquiterpene is Sesbania bispinosa The method includes the following steps: S1. Seed breaking and germination: The seeds of Sesbania sesame are mechanically broken and then placed in a humid environment at a constant temperature of 37°C to germinate. S2. Seedling Management: Plant the pre-germinated seeds in a seedling substrate made of flower nutrient soil and vermiculite in a 1:1 volume ratio, and use 50-cell seedling trays for seedling cultivation; spray with Liminong powder once during the seedling period and manage ventilation. S3. Greenhouse land preparation: In northern regions, ridges are made inside greenhouses, and the ridges are covered with black plastic film. S4. Transplanting and planting: Transplant the seedlings with their original soil to the sunny side of the ridge, maintaining the plant spacing. S5. Reproductive regulation: When sesame enters the budding stage, prune the flower buds that have formed flower buds; spray Limeinong powder during the flowering period, and maintain the minimum nighttime temperature no lower than 5℃ through temperature control measures, and control the effective light duration through light regulation measures. S6. Harvest: Manage until the seeds mature and harvest.

2. The cultivation method according to claim 1, characterized in that, In step S1, the mechanical peeling process is performed using a rice milling machine for a short period of 15 seconds.

3. The cultivation method according to claim 1, characterized in that, In step S2, the 50-cell seedling tray is made of PVC material, with a single cell capacity of 50-60 ml.

4. The cultivation method according to claim 1, characterized in that, In step S3, the width of the ridge is 50-60 cm, the height of the ridge is 15-25 cm, and the width of the furrow between the ridges is 20-30 cm.

5. The cultivation method according to claim 1, characterized in that, In step S4, the plant spacing is 50 cm.

6. The cultivation method according to claim 1, characterized in that, In step S5, the pruning process involves removing newly emerging lateral branches and compound leaves around the flower buds.

7. The cultivation method according to claim 1, characterized in that, In step S5, the temperature control measures include using a double-layered structure for insulation. The outer layer is a greenhouse, and the inner layer is equipped with a detachable small arched shed. At night, the double-layered structure is used to maintain the minimum temperature at no less than 5°C.

8. The cultivation method according to claim 1, characterized in that, In step S5, the light control measures include turning on the thermal blankets from 8:30 to 16:00 every day to control the duration of light exposure.

9. The cultivation method according to claim 1, characterized in that, In steps S2 and S5, Liminong powder is used for fertilization of seedlings and plants and for the prevention and control of diseases and pests.

Images