Regulator for improving germination rate of crop seeds and survival rate of seedlings and preparation method thereof

Abstract

The invention relates to a regulator for improving the germination rate of crop seeds and the survival rate of seedlings and a preparation method thereof, and belongs to the technical field of crop regulators. The regulator comprises the following components: 10 to 15 g / L of a film-forming humectant, 1 to 3 g / L of prickly pear polysaccharide, 6 to 10 g / L of glycerin, 0.9 to 1.2 g / L of an amino acid compound, 10 to 15 mg / L of gibberellin, 20 to 30 mg / L of salicylic acid, 0.5 to 0.8 g / L of CaCl2. 2H2O, 0.8 to 1.2 g / L of protocatechuic acid, 30 to 40 g / L of potassium fulvate, 0.3 to 0.5 g / L of ascorbic acid, 0.08 to 0.12 g / L of glutathione, 16 to 20 mg / L of a trace element compound and 5 to 6 g / L of multivitamins. The regulator can promote seed germination, improve the seed germination rate and remarkably improve the survival rate of transplanted seedlings.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of crop regulators, and relates to a regulator for improving seed germination rate and seedling survival rate of crops and a preparation method thereof. BACKGROUND

[0002] Drought is one of the most severe abiotic stresses in global agricultural production. Under the background of climate change, the frequency, intensity and duration of drought events have significantly increased, posing a serious threat to global food security. In the life cycle of crops, the seed germination and seedling stages are the key window period for determining the success of population establishment and are the most vulnerable stages to drought resistance. Seed germination is essentially a water-driven process of imbibition, activation and growth. When the soil water potential is lower than the seed germination threshold, physical imbibition is blocked, and embryo cells cannot obtain the basic swelling pressure, and the germination process is completely stalled.

[0003] Under drought conditions, not only the final germination rate is reduced, but also the germination kinetics is profoundly changed: the germination initiation time is delayed, the germination duration is prolonged, and the population germination synchrony is decreased. This "uneven germination" phenomenon leads to uneven size of seedlings in the field, asymmetric competition, and seriously affects the uniformity of the subsequent population and yield potential. How to improve the germination rate of crop seeds and the survival rate of seedlings under drought conditions has become a management problem in agricultural production. SUMMARY

[0004] In view of the above problems, the application provides a regulator and a preparation method thereof, which can significantly improve the germination rate of crop seeds and the survival rate of seedlings, and the effect is remarkable.

[0005] The application achieves the above-mentioned purposes by adopting the following technical solutions: A regulator mainly contains the following ingredients: Film-forming moisturizing agent 10-15 g / L, cactus fruit polysaccharide 1-3 g / L, glycerol 6-10 g / L, amino acid compound 0.9-1.2 g / L, gibberellin 10-15 mg / L, salicylic acid 20-30 mg / L, CaCl2·2H2O 0.5-0.8 g / L, protocatechuic acid 0.8-1.2 g / L, potassium fulvic acid 30-40 g / L, ascorbic acid 0.3-0.5 g / L, glutathione 0.08-0.12 g / L, trace element compound 16-20 mg / L, and compound vitamin 5-6 g / L.

[0006] Specifically, The film-forming moisturizing agent includes but is not limited to potassium polyacrylate, polyvinylpyrrolidone and sodium carboxymethyl cellulose.

[0007] As a preferred mode, the film-forming moisturizing agent is a mixture of potassium polyacrylate and sodium carboxymethyl cellulose.

[0008] Further preferably, the mass ratio of the potassium polyacrylate and the sodium carboxymethyl cellulose is (5-7):(5-8).

[0009] The amino acid complex contains L-proline, L-glutamic acid, glycine; As a preferred mode, the mass ratio of the L-proline, L-glutamic acid and glycine is 3:2:1.

[0010] The trace element complex contains EDTA chelated zinc and EDTA chelated iron; As a preferred mode, the mass ratio of the EDTA chelated zinc and the EDTA chelated iron is (10-12):(6-8).

[0011] The complex vitamin contains vitamin B1, vitamin B6, nicotinamide and inositol; As a preferred mode, the mass ratio of the vitamin B1, vitamin B6, nicotinamide and inositol in the complex vitamin is 2:1:1:1.

[0012] The preparation method of the cactus fruit polysaccharide is as follows: First, the cactus fruit is taken and prepared into a homogenate with an appropriate amount of water, the pH is adjusted to 4.8-5.0, cellulase is added, and enzymolysis is carried out at 50℃ for 3-4h; then mannanase is added, and enzymolysis is carried out at 50-55℃ for 2-3h, the pH is adjusted to 4.0-4.5, and then heated to 90℃ for 20-30min, cooled to room temperature, filtered, and the enzymolysis extraction liquid is obtained; Then, ethanol is added to the enzymolysis extraction liquid, the alcohol content is adjusted to 70%, and then centrifuged, and the precipitate is taken; the precipitate is washed with a 95% ethanol solution, and then dried, and the cactus fruit polysaccharide is obtained.

[0013] As a preferred mode, the addition amount of the cellulase is 0.4-0.5% of the mass of the cactus fruit; and the addition amount of the mannanase is 0.3-0.35% of the mass of the cactus fruit.

[0014] The application provides a preparation method of the regulator, and specifically comprises the following steps: Step 1, the film-forming moisturizing agent is added to an appropriate amount of 40-45℃ water, stirred at a speed of 300-500rpm for 30min until completely swollen, the cactus fruit polysaccharide, the amino acid complex, the protocatechuic acid and the potassium fulvic acid are sequentially added, and stirring is continued for 15min until completely dissolved, and solution A is obtained; Step 2, the gibberellin is dissolved in an appropriate amount of pH6.2 phosphate buffer, glycerol is added, and mixed uniformly to obtain solution B; Step 3, dissolve ascorbic acid, glutathione, trace element compound, and compound vitamin in an appropriate amount of water to obtain solution C; Step 4, slowly add solution B to solution A while maintaining the stirring speed at 500-600 rpm; then add solution C, continue stirring for 20 min, add salicylic acid and CaCl2·2H2O, dilute to volume, and homogenize to obtain the adjusting agent.

[0015] The application provides the use of the adjusting agent described above, i.e., it can be used for seed germination and seedling transplanting, and can significantly improve the seed germination rate and seedling survival rate in a drought environment.

[0016] The adjusting agent described in the application can be used in the form of seed soaking or spraying when used for seed germination, and can also be used as a coating agent.

[0017] The adjusting agent described in the application can be used in the form of seed soaking when used for seedling transplanting.

[0018] The application has the following beneficial effects: In the adjusting agent provided in the application, gibberellin can break seed dormancy and promote radicle and plumule elongation, amino acids, vitamins, etc. provide nutrients required for growth, make up for the decrease in metabolic efficiency in a drought environment, cactus fruit polysaccharide is a biological protective agent that can stabilize cell structure and prevent dehydration damage in a drought environment, glycerol, as a small-molecule polyol, can enter seed or seedling cells to regulate cells, and at the same time, as a water-absorbing and water-retaining agent, can maintain moisture; salicylic acid and protocatechuic acid can synergize with gibberellin to stimulate systemic resistance and enhance the stress resistance of crops to a drought environment.

[0019] Experiments have proved that the adjusting agent of the application can significantly improve the seed germination rate of crops in a drought environment, and can also improve the survival rate of transplanted seedlings in a drought environment. BRIEF DESCRIPTION OF DRAWINGS

[0020] Figure 1 Comparison of wheat germination test results, wherein A is wheat group 1, B is wheat group 2, C is wheat group 3, and D is wheat group 4. DETAILED DESCRIPTION

[0021] The application will be further clarified by the following examples which should not be construed as limiting the scope of the application. After reading the application, those skilled in the art will be able to modify the application in various ways and will recognize the equivalents thereof. The raw materials used in the following examples are commercially available. The zinc content of the EDTA chelated zinc is 14.7-15.3%, the iron content of the EDTA chelated iron is 12.7-13.3%; the cellulase is active enzyme with CAS number 9012-54-8 and enzyme activity of 50u / mg; the mannanase is active enzyme with CAS number 37288-54-3 and enzyme activity of 10000u / g; the cactus fruit is fresh fruit.

[0022] Example 1 The following ingredients are contained in 1L of the regulator: Film-forming moisturizing agent 10g (containing potassium polyacrylate 5g, sodium carboxymethylcellulose 5g), cactus fruit polysaccharide 3g, glycerol 6g, amino acid complex 0.9g (containing L-proline 0.45g, L-glutamic acid 0.3g, glycine 0.15g), gibberellin 15mg, salicylic acid 20mg, CaCl2·2H2O 0.8g, protocatechuic acid 0.8g, potassium fulvic acid 40g, ascorbic acid 0.5g, glutathione 0.08g, trace element complex 20mg (containing EDTA chelated zinc 12mg, EDTA chelated iron 8mg), and compound vitamin 5g (vitamin B12g, vitamin B61g, nicotinamide 1g).

[0023] The preparation method of the cactus fruit polysaccharide is as follows: First, commercially available cactus fruit is taken and prepared into a homogenate with an appropriate amount of water, the pH is adjusted to 4.8, cellulase is added (the amount of addition is 0.5% of the mass of the cactus fruit), and enzyme hydrolysis is carried out at 50°C for 3h; then mannanase is added (the amount of addition is 0.3% of the mass of the cactus fruit), and enzyme hydrolysis is carried out at 50°C for 3h, the pH is adjusted to 4.0, and then heated to 90°C for 30min, cooled to room temperature, filtered, and the enzyme hydrolysis extract is obtained; Then, ethanol is added to the enzyme hydrolysis extract, the alcohol content is adjusted to 70%, and centrifuged for 10h, and the precipitate is taken; the precipitate is washed with 95% ethanol solution, and then dried to obtain the cactus fruit polysaccharide.

[0024] Preparation of the regulator: Step 1, the film-forming moisturizing agent is added to an appropriate amount of 45°C water, stirred at a speed of 500rpm until completely swollen, and then the cactus fruit polysaccharide, amino acid complex, protocatechuic acid, and potassium fulvic acid are added in sequence, and continue to stir until completely dissolved to obtain solution A; Step 2, gibberellin is dissolved in an appropriate amount of pH 6.2 phosphate buffer, glycerol is added, mixed evenly, solution B is obtained; Step 3, ascorbic acid, glutathione, trace element compound, and complex vitamin are dissolved in an appropriate amount of water to obtain solution C; Step 4, solution B is slowly added to solution A, the stirring speed is kept at 600 rpm; solution C is then added, stirring is continued for 20 min, salicylic acid and CaCl2·2H2O are added, the volume is made up to 1 L, and homogenization treatment (3000 rpm, 10 min) is performed to form a stable solution, i.e. the conditioner is obtained.

[0025] Example 2 The conditioner contains the following ingredients per 1 L of conditioner: Film-forming moisturizing agent 15 g (containing potassium polyacrylate 7 g, sodium carboxymethyl cellulose 8 g), cactus fruit polysaccharide 1 g, glycerol 10 g, amino acid compound 1.2 g (containing L-proline 0.06 g, L-glutamic acid 0.04 g, glycine 0.02 g), gibberellin 10 mg, salicylic acid 30 mg, CaCl2·2H2O 0.5 g, protocatechuic acid 1.2 g, potassium fulvic acid 30 g, ascorbic acid 0.3 g, glutathione 0.12 g, trace element compound 16 mg (containing EDTA chelated zinc 10 mg, EDTA chelated iron 6 mg), and complex vitamin 6 g (containing vitamin B12 2.4 g, vitamin B6 1.2 g, nicotinamide 1.2 g, inositol 1.2 g).

[0026] The preparation method of the cactus fruit polysaccharide is as follows: First, commercially available cactus fruits are taken and an appropriate amount of water is added to prepare a homogenate, the pH is adjusted to 5.0, cellulase is added (the amount of cellulase added is 0.4% of the mass of the cactus fruits), and enzymatic hydrolysis is performed at 50°C for 4 h; then, mannanase is added (the amount of mannanase added is 0.35% of the mass of the cactus fruits), and enzymatic hydrolysis is performed at 55°C for 2 h, the pH is adjusted to 4.0-4.5, and then heating is performed to 90°C for 20 min, the temperature is cooled to room temperature, and filtration is performed to obtain an enzymatic hydrolysate; Then, ethanol is added to the enzymatic hydrolysate, the ethanol content is adjusted to 70%, and the mixture is allowed to stand for 8 h, centrifugation is performed, and the precipitate is taken; the precipitate is washed with a 95% ethanol solution, and then dried to obtain the cactus fruit polysaccharide.

[0027] The conditioner is prepared as follows: Step 1, the film-forming moisturizing agent is added to an appropriate amount of 40°C water, stirring is performed at a speed of 300 rpm until complete swelling, the cactus fruit polysaccharide, amino acid compound, protocatechuic acid, and potassium fulvic acid are sequentially added, and stirring is continued until complete dissolution to obtain solution A; Step 2, gibberellin is dissolved in an appropriate amount of pH 6.2 phosphate buffer, glycerol is added, and the mixture is mixed evenly to obtain solution B; Step 3, dissolve ascorbic acid, glutathione, trace element compound, and compound vitamin in an appropriate amount of water to obtain solution C; Step 4, slowly add solution B to solution A while maintaining the stirring speed at 500 rpm; then add solution C, continue stirring for 20 min, add salicylic acid and CaCl2·2H2O, dilute to 1 L, and homogenize (3000 rpm, 10 min) to form a stable solution, i.e. the conditioning agent.

[0028] Example 3 The conditioning agent contains the following ingredients per 1 L: Film-forming moisturizing agent 12 g / L (polyvinylpyrrolidone), cactus fruit polysaccharide 2 g / L, glycerol 8 g / L, amino acid compound 1.2 g / L (L-proline 0.6 g, L-glutamic acid 0.4 g, glycine 0.2 g), gibberellin 13 mg / L, salicylic acid 25 mg / L, CaCl2·2H2O 0.6 g / L, protocatechuic acid 1.0 g / L, potassium fulvic acid 35 g / L, ascorbic acid 0.4 g / L, glutathione 0.10 g / L, trace element compound 18 mg / L (EDTA chelated zinc 11, EDTA chelated iron 7), and compound vitamin 5 g / L (vitamin B1, vitamin B6, nicotinamide, and inositol in a mass ratio of 2:1:1:1).

[0029] The preparation method of the cactus fruit polysaccharide is as follows: First, take commercially available cactus fruits, add an appropriate amount of water to prepare a homogenate, adjust the pH to 5.0, add cellulase (the amount of cellulase added is 0.4% of the mass of the cactus fruits), and enzymatically hydrolyze at 50°C for 4 h; then add mannanase (the amount of mannanase added is 0.3% of the mass of the cactus fruits), and enzymatically hydrolyze at 50°C for 3 h, adjust the pH to 4.5, then heat to 90°C and maintain for 25 min, cool to room temperature, filter, and obtain an enzymatic extraction solution; Then, add ethanol to the enzymatic extraction solution, adjust the ethanol content to 70%, stand for 9 h, centrifuge, and take the precipitate; wash the precipitate with a 95% ethanol solution, and then dry to obtain the cactus fruit polysaccharide.

[0030] Preparation of the conditioning agent: Step 1, add the film-forming moisturizing agent to an appropriate amount of 45°C water, stir at a speed of 400 rpm until it is completely swollen, and then sequentially add the cactus fruit polysaccharide, amino acid compound, protocatechuic acid, and potassium fulvic acid, continue to stir until completely dissolved, and obtain solution A; Step 2, dissolve the gibberellin in an appropriate amount of pH 6.2 phosphate buffer, add glycerol, and mix well to obtain solution B; Step 3, dissolve ascorbic acid, glutathione, trace element compound, and compound vitamin in an appropriate amount of water to obtain solution C; Step 4, slowly add solution B to solution A, keep stirring speed 550 rpm; then add solution C, continue stirring for 20 min, add salicylic acid, CaCl2·2H2O, dilute to 1 L, homogenize (2500 rpm, 10 min) to form a stable solution, the conditioner is obtained.

[0031] Comparative Example 1 The following ingredients are contained in 1 L of the conditioner: Film-forming moisturizing agent 10 g (containing potassium polyacrylate 5 g, sodium carboxymethyl cellulose 5 g), glycerol 6 g, amino acid compound 0.9 g (containing L-proline 0.45 g, L-glutamic acid 0.3 g, glycine 0.15 g), gibberellin 15 mg, CaCl2·2H2O 0.8 g, protocatechuic acid 0.8 g, potassium fulvic acid 40 g, ascorbic acid 0.5 g, glutathione 0.08 g, trace element compound 20 mg (containing EDTA chelated zinc 12 mg, EDTA chelated iron 8 mg), and compound vitamin 5 g (vitamin B12 g, vitamin B6 1 g, nicotinamide 1 g).

[0032] Preparation of the conditioner: Step 1, add the film-forming moisturizing agent to an appropriate amount of 45℃ water, stir at a speed of 500 rpm until completely swollen, then add the amino acid compound, protocatechuic acid, and potassium fulvic acid in sequence, continue stirring until completely dissolved, and solution A is obtained; Step 2, dissolve the gibberellin in an appropriate amount of pH 6.2 phosphate buffer, add glycerol, and mix uniformly to obtain solution B; Step 3, dissolve the ascorbic acid, glutathione, trace element compound, and compound vitamin in an appropriate amount of water to obtain solution C; Step 4, slowly add solution B to solution A, keep stirring speed 600 rpm; then add solution C, continue stirring for 20 min, add CaCl2·2H2O, dilute to 1 L, homogenize (3000 rpm, 10 min) to form a stable solution, the conditioner is obtained.

[0033] Comparative Example 2 The following ingredients are contained in 1 L of the conditioner: Film-forming moisturizing agent 10 g (containing potassium polyacrylate 5 g, sodium carboxymethyl cellulose 5 g), cactus fruit polysaccharide 3 g, glycerol 6 g, L-proline 0.9 g, gibberellin 15 mg, salicylic acid 20 mg, CaCl2·2H2O 0.8 g, potassium fulvic acid 40 g, ascorbic acid 0.5 g, glutathione 0.08 g, trace element compound 20 mg (containing EDTA chelated zinc 12 mg, EDTA chelated iron 8 mg), and compound vitamin 5 g (vitamin B12 g, vitamin B6 1 g, nicotinamide 1 g, inositol 1 g).

[0034] The preparation method of the cactus fruit polysaccharide is as follows: Firstly, commercially available cactus fruits are taken and prepared into a homogenate with an appropriate amount of water, the pH is adjusted to 4.8, cellulase is added (the amount of cellulase added is 0.5% of the mass of the cactus fruits), and enzymatic hydrolysis is performed at 50°C for 3h; then the pH is adjusted to 4.0, and heating is performed to 90°C for 30min, and then the solution is cooled to room temperature, filtered, and an enzymatic hydrolysis extract is obtained; Then, ethanol is added to the enzymatic hydrolysis extract, the alcohol content is adjusted to 70%, and the solution is allowed to stand for 10h, and then centrifuged, and the precipitate is obtained; the precipitate is washed with a 95% ethanol solution, and then dried, and the cactus fruit polysaccharide is obtained.

[0035] Preparation of the regulator: Step 1: The film-forming humectant is added to an appropriate amount of 45°C water, and stirred at a speed of 500rpm until completely swollen, and then the cactus fruit polysaccharide, L-proline, and potassium fulvate are sequentially added, and the stirring is continued until completely dissolved, and a solution A is obtained; Step 2: The gibberellin is dissolved in an appropriate amount of pH 6.2 phosphate buffer, and glycerol is added, and the mixture is uniformly mixed, and a solution B is obtained; Step 3: The ascorbic acid, glutathione, trace element compound, and compound vitamin are dissolved in an appropriate amount of water, and a solution C is obtained; Step 4: The solution B is slowly added to the solution A, and the stirring speed is maintained at 600rpm; then the solution C is added, and the stirring is continued for 20min, and then the salicylic acid and CaCl2·2H2O are added, and the volume is adjusted to 1L, and a stable solution is obtained by homogenization treatment (3000rpm, 10min), and the regulator is obtained.

[0036] Performance test 1: Effect of the regulator on the germination of wheat and corn seeds under drought conditions 1. Test process and method The test is carried out in the laboratory of the company.

[0037] The regulator obtained in Examples 1-2 and Comparative Examples 1-2, as well as distilled water, are used as reagents to set up test groups, and the effect of the regulator is investigated by seed soaking or spraying.

[0038] 1.1 Select full and uniform wheat seeds, and after disinfection, the seeds are soaked in the following ways.

[0039] Wheat group 1 (200 seeds): the regulator of Example 1 is diluted 50 times, and the seeds are soaked for 8h; Wheat group 2 (200 seeds): the regulator of Example 2 is diluted 100 times, and the seeds are soaked for 10h; Wheat group 3 (200 seeds): the regulator of Comparative Example 1 is diluted 50 times, and the seeds are soaked for 10h; Wheat group 4 (200 seeds): distilled water is used for seed soaking for 10h.

[0040] After the seed soaking is completed, the wheat seeds are laid on filter paper culture dishes, which are then placed in an artificial climate incubator with a temperature setting of 28°C. Distilled water is supplied every 24 hours to keep the filter paper moist. After 3 days of cultivation, the seed germination is counted.

[0041] 1.2 Full and uniform corn seeds are selected and sterilized, and then soaked according to the following methods.

[0042] Corn Group 1 (150 seeds): distilled water soaking for 10 hours + spraying of the adjusting agent of Example 1 diluted 50 times; Corn Group 2 (150 seeds): distilled water soaking for 10 hours + spraying of the adjusting agent of Example 2 diluted 100 times; Corn Group 3 (150 seeds): distilled water soaking for 10 hours + soaking for 10 hours after the adjusting agent of Comparative Example 2 is diluted 50 times; Corn Group 4 (150 seeds): distilled water soaking for 10 hours + spraying of distilled water.

[0043] After the seed soaking is completed, the corn seeds are laid on filter paper culture dishes, which are then placed in an artificial climate incubator with a temperature setting of 28°C. The adjusting agent or distilled water is sprayed according to different treatment methods every 24 hours to keep the filter paper moist. The seed germination is counted.

[0044] 2. Results and analysis As can be seen from Table 1, the germination rate of wheat is significantly improved after the wheat seeds are soaked with the adjusting agents of Example 1 and Example 2, and the germination time is shortened. The effect is better than that of the adjusting agent of Comparative Example 1 and distilled water soaking.

[0045] Table 1 Effect of different treatments on wheat seed germination

[0046] Note: P < 0.05 compared with Corn Group 4.

[0047] As can be seen from Table 2, the germination rate of corn is significantly increased after the corn seeds are sprayed with the adjusting agents of Example 1 and Example 2, and the effect is better than that of Comparative Example 2 and distilled water spraying.

[0048] Table 2 Effect of different treatments on corn seed germination

[0049] Note: P < 0.05 compared with Corn Group 4.

[0050] From the results in Table 1, Table 2, it can be seen that the spraying method can promote the seed vigor of crops more than the seed soaking method, can obviously shorten the germination time, and can improve the germination rate of crop seeds; and the adjusting agent provided by the present application has a promoting effect equivalent to that of the adjusting agent obtained by diluting 50 times when used for spraying, but is better than the comparative examples and distilled water treatment. The components in Comparative Example 1 and Comparative Example 2 are different from those in the present application, and they also have the effect of promoting seed germination, but are poorer than the present application, indicating that the adjusting agent of the present application can significantly improve the germination rate of crop seeds.

[0051] Performance test two: effect of adjusting agent on survival rate of strawberry transplanted seedlings under drought conditions 1. Test process and method Take strawberry plug seedlings with basically consistent growth vigor, set up 5 treatments, 30 plug seedlings for each treatment. The plug seedlings are treated in the following manner and then transplanted into culture pots, 1 plant per pot.

[0052] Treatment method: Strawberry group 1: the adjusting agent of Example 1 is diluted 50 times and soaked for 8 hours; Strawberry group 2: the adjusting agent of Example 2 is diluted 50 times and soaked for 10 hours; Strawberry group 3: the adjusting agent of Comparative Example 1 is diluted 50 times and soaked for 10 hours; Strawberry group 4: distilled water is soaked for 10 hours; Strawberry group 5: distilled water is soaked for 10 hours.

[0053] After transplanting, strawberry group 1, strawberry group 3, and strawberry group 4 are subjected to moderate drought treatment with 30% relative water content, and strawberry group 2 and strawberry group 5 are subjected to mild drought treatment with 50% relative water content. The survival rate after transplanting is counted.

[0054] 2. Results and analysis As can be seen from Table 3, the adjusting agents obtained by using Example 1 and Example 2 to soak the strawberry plug seedlings can significantly improve the survival rate of the seedlings in a drought environment. In a mild drought environment, the survival rate of the plug seedlings treated with the adjusting agent of Example 2 is above 95%, and the survival rate is better than that in moderate drought treatment; but in moderate drought treatment, the survival rate of the plug seedlings treated with the adjusting agent of Example 1 is above 85%, which is significantly higher than that of the adjusting agent obtained by using Comparative Example 2. Overall, the survival rate of transplanting in a mild drought environment is significantly higher than that in a moderate drought environment, and the use of the adjusting agent of the present application can significantly improve the survival rate of the seedlings in a drought environment, and the effect is significant.

[0055] Table 3 Effect of different treatments on survival rate of strawberry transplanted seedlings

[0056] Note: P<0.05 compared to strawberry 4 group.

Claims

1. A regulator, characterized in that, It contains the following ingredients: Film-forming moisturizer 10-15g / L, cactus fruit polysaccharide 1-3g / L, glycerin 6-10g / L, amino acid complex 0.9-1.2g / L, gibberellin 10-15mg / L, salicylic acid 20-30mg / L, CaCl2·2H2O 0.5-0.8g / L, protocatechuic acid 0.8-1.2g / L, potassium humate 30-40g / L, ascorbic acid 0.3-0.5g / L, glutathione 0.08-0.12g / L, trace element complex 16-20mg / L, complex vitamins 5-6g / L.

2. The regulator as described in claim 1, characterized in that, The film-forming moisturizing agent is one or a mixture of potassium polyacrylate, polyvinylpyrrolidone, and sodium carboxymethyl cellulose.

3. The regulator as described in claim 2, characterized in that, The film-forming moisturizer is a mixture of potassium polyacrylate and sodium carboxymethyl cellulose in a mass ratio of (5-7):(5-8).

4. The regulator as described in claim 1, characterized in that, The amino acid complex contains L-proline, L-glutamic acid, and glycine in a mass ratio of 3:2:

1.

5. The regulator as described in claim 1, characterized in that, The trace element complex contains EDTA-chelated zinc and EDTA-chelated iron in a mass ratio of (10-12):(6-8).

6. The regulator as described in claim 1, characterized in that, The compound vitamin contains vitamin B1, vitamin B6, nicotinamide, and inositol in a mass ratio of 2:1:1:

1.

7. The regulator as described in claim 1, characterized in that, The preparation method of the cactus fruit polysaccharide is as follows: First, take cactus fruit, add water to prepare a homogenate, adjust the pH to 4.8-5.0, add cellulase, and enzymatically hydrolyze at 50℃ for 3-4 hours; then add mannanase, and enzymatically hydrolyze at 50-55℃ for 2-3 hours, adjust the pH to 4.0-4.5, then heat to 90℃ and keep warm for 20-30 minutes, cool to room temperature, filter, and obtain the enzymatic extract; Then, ethanol was added to the enzymatic extract to adjust the alcohol content to 70%, and the mixture was allowed to stand for 8-10 hours. After centrifugation, the precipitate was collected. The precipitate was washed with a 95% ethanol solution and then dried to obtain cactus fruit polysaccharide.

8. The regulator as described in claim 7, characterized in that, In the method for preparing prickly pear fruit polysaccharide, the amount of cellulase added is 0.4-0.5% of the weight of the prickly pear fruit; the amount of mannanase added is 0.3-0.35% of the weight of the prickly pear fruit.

9. The regulator according to any one of claims 1-8, characterized in that, The preparation method of the regulator includes the following steps: Step 1: Add the film-forming moisturizing agent to an appropriate amount of water at 40-45℃, stir at 300-500 rpm for 30 minutes until completely swollen, add prickly pear fruit polysaccharide, amino acid complex, protocatechuic acid, and potassium humate in sequence, continue stirring for 15 minutes, add the film-forming agent, and stir until completely dissolved to obtain solution A. Step 2: Dissolve gibberellin in an appropriate amount of phosphate buffer solution with pH 6.2, add glycerol, mix well, and obtain solution B; Step 3: Dissolve ascorbic acid, glutathione, trace element complex, and compound vitamins in an appropriate amount of water to obtain solution C; Step 4: Slowly add solution B to solution A while maintaining a stirring speed of 500-600 rpm; then add solution C and continue stirring for 20 minutes. Add salicylic acid and CaCl2·2H2O, and bring the volume to 1 L. Homogenize to obtain the regulator.

10. Use of the regulator as described in any one of claims 1-9 in improving seed germination rate and seedling survival rate.

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