Repairing and relieving composition suitable for sensitive skin and application thereof

Abstract

The invention relates to a composition for repairing and relieving sensitive skin and application of the composition, and belongs to the technical field of cosmetics. The invention provides a composition for repairing and relieving sensitive skin, which is prepared by compounding sericin with a tilia europaea extract and a kadsura longepedunculata extract, the sericin is obtained by extraction and purification after supercritical CO2 treatment, and plays a leading role by repairing a physical barrier and moisturizing; the European tilia flower extract can quickly relieve nerve discomfort; the kadsura longepedunculata extract can scavenge free radicals and resist environmental pressure; the three components synergistically play a role from different paths, so that all-around and multi-target rapid repair and lasting soothing of sensitive skin are realized, and the effect is obviously superior to that of a single component or a traditional composition.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology and relates to a repairing and soothing composition suitable for sensitive skin and its application. Background Technology

[0002] With increasing environmental pressure and a faster pace of life, the number of people with sensitive skin is rising year by year. The main characteristics of sensitive skin are impaired skin barrier function and reduced tolerance to external stimuli (such as cosmetics, ultraviolet rays, and temperature changes), easily leading to burning, stinging, itching, and tightness. Therefore, the market urgently needs cosmetics that can effectively soothe skin discomfort and repair damaged skin barriers.

[0003] Currently, most soothing and repairing products on the market rely on classic ingredients such as ceramides, centella asiatica, and purslane. However, the efficacy of these ingredients tends to be homogenized, and their repair speed and soothing effects remain limited for some severely sensitive individuals or those with specific constitutions. Sericin, as a natural protein, possesses excellent moisturizing, antioxidant, and cell compatibility properties, making it an ideal skincare ingredient. However, traditional sericin extraction methods (such as hot water extraction and acid-base extraction) have significant drawbacks: high temperatures or extreme pH conditions severely damage the natural three-dimensional structure of sericin, leading to a substantial reduction in its bioactivity and even the risk of sensitization. This greatly limits its application in high-end sensitive skin care products.

[0004] Furthermore, European linden flower extract is renowned for its excellent soothing and anti-inflammatory properties, which can alleviate skin sensitivity and discomfort; while Schisandra chinensis extract is rich in antioxidants such as lignans, which can effectively combat oxidative stress and reduce skin damage. How to scientifically combine functional ingredients with different bioactivities to form synergistic compositions is a technical problem that those skilled in the art have long been dedicated to solving. Summary of the Invention

[0005] The purpose of this invention is to provide a repairing and soothing composition suitable for sensitive skin and its application. This invention scientifically combines sericin, linden flower extract, and schisandra chinensis extract. The sericin is extracted and purified through supercritical CO2 treatment and plays a leading role in repairing the physical barrier and moisturizing. Linden flower extract can quickly soothe nerve discomfort. Schisandra chinensis extract can scavenge free radicals and combat environmental stress. The three work synergistically through different pathways to achieve comprehensive, multi-target rapid repair and long-lasting soothing of sensitive skin, with significantly better effects than single ingredients or traditional combinations.

[0006] The objective of this invention can be achieved through the following technical solutions: In a first aspect, the present invention provides a repairing and soothing composition suitable for sensitive skin, the composition comprising the following components: sericin, linden flower extract, and schisandra chinensis extract, wherein the weight ratio of sericin, linden flower extract, and schisandra chinensis extract is (0.01-1):(0.1-3):(0.01-1).

[0007] Preferably, the weight ratio of the sericin, European linden flower extract, and Schisandra chinensis extract is (0.05-0.5):(0.5-2):(0.1-0.6).

[0008] More preferably, the weight ratio of the sericin, European linden flower extract, and Schisandra chinensis extract is (0.1-0.3):(0.8-1.2):(0.2-0.3).

[0009] More preferably, the sericin is obtained by extraction and purification after supercritical CO2 treatment.

[0010] In a second aspect, the present invention provides a method for preparing sericin in the composition described in the first aspect using supercritical CO2 treatment combined with extraction and purification technology, specifically comprising the following steps: S1. After washing the silkworm cocoons with pure water, use an ethanol solution for preliminary degreasing, then dry the degreased cocoon material, pulverize it through a 40-80 mesh sieve to obtain silkworm cocoon powder. S2. Load the silkworm cocoon powder into the extraction vessel with a filling coefficient of 60%-75%, close the extraction vessel, introduce supercritical CO2 fluid, start the high-pressure pump to pressurize, and heat the system to the set temperature. After the system stabilizes, pump in the entrainer for dynamic circulation treatment to obtain the treated fluid mixture. S3. The treated fluid mixture is sequentially introduced into the primary separation vessel and the secondary separation vessel. The entrained agent is separated and recovered by depressurization and heating. The CO2 is condensed and recycled to obtain silkworm cocoon solid material that has been deeply degreased and activated. S4. The silkworm cocoon solid material that has been deeply degreased and activated is stirred and extracted with the extraction solvent under mild conditions to dissolve the sericin. Then, the solid and liquid are separated to obtain a crude sericin extract. The crude extract is transferred to a vacuum rotary evaporator for concentration to obtain a dilute sericin aqueous solution. S5. Pump the dilute sericin aqueous solution into the nanofiltration membrane system for concentration and dialysis at room temperature to obtain nanofiltration concentrated sericin protein solution. S6. The nanofiltration concentrated sericin solution is filtered through a 0.22μm microporous membrane and then freeze-dried to obtain sericin.

[0011] Preferably, in step S1, the ratio of silkworm cocoons to ethanol solution is 1g:10-30mL, the concentration of the ethanol solution is 60-70% (v / v), the preliminary degreasing is carried out by stirring at 25℃-35℃ for 0.5-1.5h, and the drying is carried out at 35℃-45℃ and a vacuum degree below -0.09MPa for 12-36h until the moisture content of the raw material is below 5%.

[0012] More preferably, the ratio of silkworm cocoons to ethanol solution is 1g:15mL, and the concentration of ethanol solution is 65% (v / v).

[0013] This step primarily removes wax and grease from the surface of the silk, and the gentle ethanol degreasing also avoids damaging the sericin.

[0014] Preferably, the supercritical CO2 treatment in step S2 is carried out under the conditions of pressure 25MPa-45MPa, temperature 35℃-45℃, and CO2 flow rate of 15-35 L / h. The entrainer is a 20%-50% (v / v) ethanol solution, the flow rate-to-mass ratio of the entrainer to supercritical CO2 is 1:(15-40), and the dynamic circulation extraction time is 1.5-4h.

[0015] More preferably, the supercritical CO2 treatment in step S2 is carried out at 30℃-40℃, pressure 35MPa-40MPa, and CO2 flow rate of 20-25L / h, the entrainer is a 30%-40% (v / v) ethanol solution, and the dynamic circulation extraction time is 3h.

[0016] Preferably, the separation temperature and pressure of the primary separation vessel in step S3 are 40℃-50℃ and 6MPa-15MPa, respectively, and the separation temperature and pressure of the secondary separation vessel are 35℃-45℃ and 4MPa-7MPa, respectively.

[0017] Through the above steps, the high permeability and selective dissolution capacity of supercritical CO2 in conjunction with the entrainer are utilized to achieve deep degreasing of silkworm cocoon raw materials at a mild temperature and effectively remove small molecule impurities such as pigments. At the same time, the permeation and swelling effect of the high-pressure fluid "activates" the silkworm cocoon matrix and weakens the binding between sericin and fibroin, thus creating the best conditions for the subsequent efficient and mild dissolution and extraction of highly active sericin protein.

[0018] Preferably, the extraction method of sericin in step S4 is as follows: the silkworm cocoon solid material that has undergone deep defatting and activation is transferred to an extraction tank, the silkworm cocoon solid material that has undergone deep defatting and activation is mixed with the extraction solvent at a material-liquid ratio of 1g:15-25mL, deionized water is used as the extraction solvent, and the mixture is stirred and extracted for 2-4 hours at 40-50℃ and 50-150 rpm. After extraction, the mixture is centrifuged at 3000-5000 rpm for 10-20 minutes at 30-45℃, the clear liquid is collected to obtain crude sericin extract, and then concentrated at 30℃-40℃, -0.08MPa to -0.1MPa vacuum, and 60-100 rpm for 40-90 minutes to obtain a dilute sericin aqueous solution.

[0019] More preferably, in step S4, the ratio of the deep-degreased and activated silkworm cocoon solid material to the extraction solvent is 1 g: 20 mL, the stirring extraction is carried out at a temperature of 45-48℃ and a rotation speed of 80-120 rpm for 2.5-3.5 h, and the concentration is carried out at a temperature of 35℃-40℃ for 70-80 min.

[0020] This extraction process is conducted under mild conditions, which effectively prevents the sericin from denaturing due to localized overheating.

[0021] Preferably, the room temperature concentration and dialysis in step S5 is performed using a nanofiltration membrane made of polyamide, polypiperazine amide, or sulfonated polyethersulfone, with a molecular weight cutoff of 200 Da-800 Da, under the conditions of a temperature of 15℃-35℃, a pressure of 0.8MPa-2.0MPa, and a transmembrane flow rate of 15 L / h-30 L / h, to circulate and concentrate the membrane until the solid content is 10%-20%.

[0022] More preferably, the nanofiltration membrane in step S5 is a polyamide nanofiltration membrane, with a temperature of 20℃-30℃, a pressure of 1.0MPa-1.5MPa, and is cyclically concentrated to a solid content of 12%-15%.

[0023] The above steps can completely retain sericin and its polypeptides with a molecular weight greater than 500 Da, while allowing inorganic salts (such as trace amounts of sodium salt) and impurities such as pigments and odor molecules with a molecular weight less than 200 Da to pass through, thus achieving desalting, decolorizing and deodorizing of the sericin solution.

[0024] Preferably, the microporous filter membrane in step S6 is a polyethersulfone or polyvinylidene fluoride microporous filter membrane, and the freeze drying is carried out under conditions where the condenser temperature is below -50°C and the vacuum degree is below 10 Pa, until the moisture content of the material is below 5%.

[0025] The sericin prepared by the above-mentioned specific method has the characteristics of intact molecular structure, high biological activity, no solvent residue, and concentrated molecular weight distribution, which can ensure that the composition plays a gentle and effective role in repairing and soothing sensitive skin.

[0026] In a third aspect, the present invention provides the application of a repairing and soothing composition suitable for sensitive skin in skincare products, the skincare products including toners, lotions, creams, masks, serums, and sprays, and the amount of the composition added is 0.5%-3% of the total weight of the skincare products.

[0027] In a fourth aspect, the present invention provides a serum comprising the following ingredients by weight percentage: 0.5%-3% of the repairing and soothing composition suitable for sensitive skin as described in the first aspect, 0.05%-0.3% of a thickener, 0.5%-10% of a moisturizer, 0.5%-5% of a preservative, 0.01%-0.3% of a pH adjuster, and the balance being deionized water.

[0028] Preferably, the thickener includes at least one of xanthan gum, carbomer, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, ammonium acryloyldimethyl taurate / VP copolymer, and sclerotium gum; Preferably, the moisturizer includes at least one of allantoin, betaine, β-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,3-butanediol, glycerin, D-panthenol, and ceramide. Preferably, the pH adjuster includes at least one of arginine and tromethamine; Preferably, the preservative includes at least one of 1,3-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone.

[0029] The beneficial effects of this invention are: (1) This invention scientifically combines sericin, European linden flower extract and Schisandra chinensis extract. The sericin is extracted and purified after supercritical CO2 treatment and plays a leading role in repairing the physical barrier and moisturizing. European linden flower extract can quickly soothe nerve discomfort. Schisandra chinensis extract can eliminate free radicals and fight environmental stress. The three work synergistically through different pathways to achieve comprehensive and multi-target rapid repair and long-lasting soothing of sensitive skin. The effect is significantly better than single ingredients or traditional combinations.

[0030] (2) This invention uses supercritical CO2 fluid to further degrease and activate the silkworm cocoon raw material after preliminary degreasing. This process is carried out under mild conditions, which can deeply remove lipid impurities and activate the sericin matrix, avoiding protein structure damage. Subsequently, highly active sericin protein is obtained through mild liquid-phase extraction and advanced membrane separation and freeze-drying technology. The entire process of this invention is carried out in a low-temperature environment, which perfectly avoids the problems of protein denaturation and loss of activity caused by traditional methods. Moreover, the sericin protein obtained by this method has an intact molecular structure, extremely high biological activity, pure white color, no odor, and no chemical solvent residue. Its gentleness is particularly suitable for use on sensitive skin.

[0031] (3) All components of the composition provided by the present invention are derived from natural sources, have low irritation and high safety, and are suitable for all types of sensitive skin people, including those with severe sensitive skin. Attached Figure Description

[0032] Figure 1 The cell healing status of Example 1 and Comparative Example 2 at 0h and 48h; Figure 2 This is a schematic diagram illustrating the irritation of Example 1 and Comparative Example 2. Detailed Implementation

[0033] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with embodiments, is provided below.

[0034] The silkworm cocoons were purchased from Guangzhou Baicaoshangpin Trading Co., Ltd. The European linden flower extract is from Henan Tianfu Chemical Co., Ltd., CAS number 84929-52-2; The Schisandra chinensis extract is from Draco, and its trade name is Schisandra chinensis extract.

[0035] Unless otherwise specified, the conditions in the examples shall be performed under conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, the components of the formulations in the examples are all commercially available products.

[0036] Unless otherwise specified, all other materials, reagents, etc. used in all embodiments and comparative examples of this invention are commercially available.

[0037] The components and formulations of the repair and soothing compositions of Examples 1-13 and Comparative Examples 1-14 are shown in Table 1, and the total mass of each composition is equal. First, sericin was extracted and purified by supercritical CO2 treatment according to each specific implementation plan. Then, the components in the compositions of each example and each comparative example were mixed according to the formulated amounts to obtain the repair and soothing compositions of Examples 1-13 and Comparative Examples 1-14, respectively.

[0038] The sericin in Examples 1, 10-13, and Comparative Examples 11-14 was extracted and purified after supercritical CO2 treatment, specifically including the following steps: S1. After washing the silkworm cocoons three times with pure water, mix them with a 65% (v / v) ethanol solution at a material-to-liquid ratio of 1g:15mL. Stir at 30℃ for 1h for preliminary degreasing. Then place them in a vacuum drying oven and dry at 40℃ and a vacuum degree below -0.09 MPa for 30h until the moisture content of the raw material is below 5%. Grind the raw material through a 60-mesh sieve to obtain silkworm cocoon powder. S2. The silkworm cocoon powder is loaded into the extraction vessel with a filling coefficient of 65%. The extraction vessel is closed, and supercritical CO2 fluid is introduced at a flow rate of 22 L / h. The high-pressure pump is started to raise the system pressure to 38 MPa, and then the system temperature is raised to 40℃. After the system stabilizes, 35% (v / v) ethanol aqueous solution is pumped in at a flow-to-mass ratio of 1:25 as an entrainer. Under these conditions, the mixture is dynamically circulated for 3 hours to obtain the treated fluid mixture. S3. The treated fluid mixture is introduced into the primary separation vessel, where the entrainer is separated from most of the CO2 at 47°C and 9 MPa. The entrainer rich in impurities is discharged from the bottom of the vessel for recovery. The remaining fluid enters the secondary separation vessel, where the residual entrainer is recovered at 40°C and 5 MPa. The CO2 is condensed and recycled. Deeply degreased and activated silkworm cocoon solids are collected at the bottom of the separation vessel. S4. Transfer the deep-degreased and activated silkworm cocoon solid material to the extraction tank. Mix the deep-degreased and activated silkworm cocoon solid material with the extraction solvent at a material-to-liquid ratio of 1 g: 20 mL. Use deionized water as the extraction solvent. Stir and extract for 3 h at 100 rpm at 47℃. After extraction, centrifuge at 4000 rpm for 15 min at 40℃. Collect the clear liquid to obtain crude sericin extract. Then concentrate it at 35℃, -0.09 MPa vacuum, and 80 rpm for 80 min to obtain a dilute sericin aqueous solution. S5. Pump the diluted sericin aqueous solution into the nanofiltration membrane system, use a polyamide nanofiltration membrane, select a molecular weight cutoff of 500 Da, and circulate and concentrate it to a solid content of 15% under the conditions of temperature of 25℃, pressure of 1.2MPa, and transmembrane flow rate of 22L / h to obtain the nanofiltration concentrated sericin protein solution. S6. After the nanofiltration concentrated sericin solution is filtered through a 0.22μm microporous membrane, it is placed in a freeze dryer and freeze-dried at a condenser temperature of -55℃ and a vacuum degree of 8 Pa until the moisture content of the material is less than 5%, and finally white to off-white sericin is obtained and sealed for storage.

[0039] Example 2 Compared with Example 1, the only difference is that in Example 2, step S1 in the preparation of sericin is as follows: after washing the silkworm cocoons three times with pure water, they are mixed with a 70% (v / v) ethanol solution at a material-to-liquid ratio of 1g:15mL, stirred at 35°C for 1.5h for preliminary degreasing, and then placed in a vacuum drying oven and dried at 45°C and a vacuum degree below -0.09 MPa for 24h until the moisture content of the raw material is below 5%, and then pulverized through a 60-mesh sieve to obtain silkworm cocoon powder.

[0040] Example 3 Compared with Example 1, the only difference is that in Example 3, step S2 in the preparation of sericin is as follows: silkworm cocoon powder is loaded into the extraction vessel with a filling coefficient of 60%, the extraction vessel is closed, supercritical CO2 fluid is introduced at a flow rate of 22 L / h, and the high-pressure pump is started to raise the system pressure to 45 MPa. Then the system temperature is raised to 35°C. After the system stabilizes, 35% (v / v) ethanol aqueous solution is pumped in at a flow-to-mass ratio of 1:25 as an entrainer. Under these conditions, the mixture is dynamically circulated for 3 h to obtain the treated fluid mixture.

[0041] Example 4 Compared with Example 1, the only difference is that in Example 4, step S2 in the preparation of sericin is as follows: silkworm cocoon powder is loaded into the extraction vessel with a filling coefficient of 65%, the extraction vessel is closed, supercritical CO2 fluid is introduced at a flow rate of 22 L / h, and the high-pressure pump is started to raise the system pressure to 25 MPa. Then the system temperature is raised to 45°C. After the system stabilizes, 35% (v / v) ethanol aqueous solution is pumped in as an entrainer at a flow-to-mass ratio of 1:25. Under these conditions, the mixture is dynamically circulated for 3 h to obtain the treated fluid mixture.

[0042] Example 5 Compared with Example 1, the only difference is that in Example 5, step S2 in the preparation of sericin is as follows: silkworm cocoon powder is loaded into the extraction vessel with a filling coefficient of 65%, the extraction vessel is closed, supercritical CO2 fluid is introduced at a flow rate of 22 L / h, and the high-pressure pump is started to raise the system pressure to 38 MPa. Then the system temperature is raised to 40°C. After the system stabilizes, 30% (v / v) ethanol aqueous solution is pumped in as an entrainer at a flow-to-mass ratio of 1:25. Under these conditions, the mixture is dynamically circulated for 3 h to obtain the treated fluid mixture.

[0043] Example 6 Compared with Example 1, the only difference is that in Example 6, step S2 in the preparation of sericin is as follows: silkworm cocoon powder is loaded into the extraction vessel with a filling coefficient of 70%, the extraction vessel is closed, supercritical CO2 fluid is introduced at a flow rate of 22 L / h, and the high-pressure pump is started to raise the system pressure to 38 MPa. Then the system temperature is raised to 40°C. After the system stabilizes, 40% (v / v) ethanol aqueous solution is pumped in at a flow-to-mass ratio of 1:25 as an entrainer. Under these conditions, the mixture is dynamically circulated for 3 h to obtain the treated fluid mixture.

[0044] Example 7 Compared with Example 1, the only difference is that in Example 7, step S3 in the preparation of sericin is specifically as follows: the treated fluid mixture is introduced into a primary separation vessel, and the entrainer is separated from most of the CO2 at 50°C and 6MPa. The entrainer rich in impurities is discharged from the bottom of the vessel for recovery. The remaining fluid enters a secondary separation vessel, and the residual entrainer is recovered at 35°C and 7MPa. The CO2 is condensed and recycled. The silkworm cocoon solid material that has undergone deep degreasing and activation is collected at the bottom of the separation vessel.

[0045] Example 8 Compared with Example 1, the only difference is that in Example 8, step S4 in the preparation of sericin is as follows: the silkworm cocoon solid material that has undergone deep defatting and activation is transferred to the extraction tank, the silkworm cocoon solid material that has undergone deep defatting and activation is mixed with the extraction solvent at a material-liquid ratio of 1 g: 20 mL, deionized water is used as the extraction solvent, and the mixture is stirred and extracted at 40°C and 150 rpm for 3.5 h. After extraction, the mixture is centrifuged at 40°C and 4000 rpm for 15 min, the clear liquid is collected, and the crude sericin extract is obtained. Then, the extract is concentrated at 40°C, -0.1 MPa vacuum, and 100 rpm for 70 min to obtain a dilute sericin aqueous solution.

[0046] Example 9 Compared with Example 1, the only difference is that in Example 9, step S4 in the preparation of sericin is as follows: the silkworm cocoon solid material that has undergone deep defatting and activation is transferred to the extraction tank, the silkworm cocoon solid material that has undergone deep defatting and activation is mixed with the extraction solvent at a material-liquid ratio of 1 g: 20 mL, deionized water is used as the extraction solvent, and the mixture is stirred and extracted at 50°C and 50 rpm for 2.5 h. After extraction, the mixture is centrifuged at 40°C and 4000 rpm for 15 min, the clear liquid is collected, and the crude sericin extract is obtained. Then, the extract is concentrated at 40°C, -0.08 MPa vacuum, and 100 rpm for 80 min to obtain a dilute sericin aqueous solution.

[0047] Comparative Example 1 Compared with Example 1, the only difference is that in the preparation of sericin in Comparative Example 1, step S1 is as follows: after washing the silkworm cocoons three times with pure water, they are mixed with 80% (v / v) ethanol solution at a material-to-liquid ratio of 1g:15mL, stirred at 30°C for 1h for preliminary degreasing, and then placed in a vacuum drying oven and dried at 40°C and a vacuum degree below -0.09 MPa for 30h until the moisture content of the raw material is below 5%, and then pulverized through a 60-mesh sieve to obtain silkworm cocoon powder.

[0048] Comparative Example 2 Compared with Example 1, the only difference is that in Comparative Example 2, step S1 in the preparation of sericin is as follows: the silkworm cocoons are washed three times with pure water and then placed in a vacuum drying oven and dried for 30 hours at 40°C and a vacuum degree of less than -0.09 MPa until the moisture content of the raw material is less than 5%. The raw material is then pulverized through a 60-mesh sieve to obtain silkworm cocoon powder.

[0049] Comparative Example 3 Compared with Example 1, the only difference is that in Comparative Example 3, step S2 in the preparation of sericin is as follows: silkworm cocoon powder is loaded into the extraction vessel with a filling coefficient of 65%, the extraction vessel is closed, supercritical CO2 fluid is introduced at a flow rate of 22 L / h, and the high-pressure pump is started to raise the system pressure to 20 MPa. Then the system temperature is raised to 40°C. After the system stabilizes, 35% (v / v) ethanol aqueous solution is pumped in at a flow-to-mass ratio of 1:25 as an entrainer. Under these conditions, the mixture is dynamically circulated for 3 hours to obtain the treated fluid mixture.

[0050] Comparative Example 4 Compared with Example 1, the only difference is that in Comparative Example 4, step S2 in the preparation of sericin is as follows: silkworm cocoon powder is loaded into the extraction vessel with a filling coefficient of 65%, the extraction vessel is closed, supercritical CO2 fluid is introduced at a flow rate of 22 L / h, and the high-pressure pump is started to raise the system pressure to 50 MPa. Then the system temperature is raised to 40°C. After the system stabilizes, 35% (v / v) ethanol aqueous solution is pumped in at a flow-to-mass ratio of 1:25 as an entrainer. Under these conditions, the mixture is dynamically circulated for 3 hours to obtain the treated fluid mixture.

[0051] Comparative Example 5 Compared with Example 1, the only difference is that in Comparative Example 5, step S2 in the preparation of sericin is as follows: silkworm cocoon powder is loaded into the extraction vessel with a filling coefficient of 65%, the extraction vessel is closed, supercritical CO2 fluid is introduced at a flow rate of 22 L / h, and the high-pressure pump is started to raise the system pressure to 38 MPa. Then the system temperature is raised to 55°C. After the system stabilizes, 15% (v / v) ethanol aqueous solution is pumped in as an entrainer at a flow-to-mass ratio of 1:25. Under these conditions, the mixture is dynamically circulated for 3 hours to obtain the treated fluid mixture.

[0052] Comparative Example 6 Compared with Example 1, the only difference is that in Comparative Example 6, step S2 in the preparation of sericin is as follows: silkworm cocoon powder is loaded into the extraction vessel with a filling coefficient of 65%, the extraction vessel is closed, supercritical CO2 fluid is introduced at a flow rate of 22 L / h, and the high-pressure pump is started to raise the system pressure to 38 MPa. Then the system temperature is raised to 40°C. After the system stabilizes, 55% (v / v) ethanol aqueous solution is pumped in at a flow-to-mass ratio of 1:25 as an entrainer. Under these conditions, the mixture is dynamically circulated for 3 hours to obtain the treated fluid mixture.

[0053] Comparative Example 7 Compared with Example 1, the only difference is that step S4 in the preparation of sericin in Comparative Example 7 is as follows: the silkworm cocoon solid material that has undergone deep defatting and activation is transferred to the extraction tank, the silkworm cocoon solid material that has undergone deep defatting and activation is mixed with the extraction solvent at a material-liquid ratio of 1 g: 20 mL, deionized water is used as the extraction solvent, and the mixture is stirred and extracted at 60°C and 100 rpm for 3 h. After extraction, the mixture is centrifuged at 40°C and 4000 rpm for 15 min, the clear liquid is collected, and the crude sericin extract is obtained. Then, the extract is concentrated at 35°C, -0.09 MPa vacuum, and 80 rpm for 80 min to obtain a dilute sericin aqueous solution.

[0054] Comparative Example 8 Compared with Example 1, the only difference is that steps S2 and S3 were omitted in the preparation of sericin in Comparative Example 8. Specifically, it includes the following steps: S1. After washing the silkworm cocoons three times with pure water, mix them with a 65% (v / v) ethanol solution at a material-to-liquid ratio of 1g:15mL. Stir at 30℃ for 1h for preliminary degreasing. Then place them in a vacuum drying oven and dry at 40℃ and a vacuum degree below -0.09 MPa for 30h until the moisture content of the raw material is below 5%. Grind the raw material through a 60-mesh sieve to obtain silkworm cocoon powder. S2. Mix silkworm cocoon powder with deionized water at a ratio of 1 g: 20 mL. Stir and extract at 47℃ and 100 rpm for 3 h. After extraction, centrifuge at 4000 rpm for 15 min at 40℃, collect the clear liquid to obtain crude sericin extract, and then concentrate at 35℃, -0.09 MPa vacuum and 80 rpm for 80 min to obtain dilute sericin aqueous solution. S3. Pump the diluted sericin aqueous solution into the nanofiltration membrane system, use a polyamide nanofiltration membrane, select a molecular weight cutoff of 500 Da, and circulate and concentrate it to a solid content of 15% under the conditions of temperature of 25℃, pressure of 1.2MPa, and transmembrane flow rate of 22L / h to obtain the nanofiltration concentrated sericin protein solution. S4. After the nanofiltration concentrated sericin solution is filtered through a 0.22μm microporous membrane, it is placed in a freeze dryer and freeze-dried at a condenser temperature of -55℃ and a vacuum degree of 8 Pa until the moisture content of the material is less than 5%, and finally white to off-white sericin is obtained and sealed for storage.

[0055] Comparative Example 9 Compared with Example 1, the only difference is that in Comparative Example 9, hot water alkaline extraction was used instead of steps S2, S3, and S4 in the preparation of sericin. Specifically, it includes the following steps: S1. After washing the silkworm cocoons three times with pure water, mix them with a 65% (v / v) ethanol solution at a material-to-liquid ratio of 1g:15mL. Stir at 30℃ for 1h for preliminary degreasing. Then place them in a vacuum drying oven and dry at 40℃ and a vacuum degree below -0.09 MPa for 30h until the moisture content of the raw material is below 5%. Grind the raw material through a 60-mesh sieve to obtain silkworm cocoon powder. S2. Mix silkworm cocoon powder and deionized water at a ratio of 1g:20ml, place in a reaction vessel equipped with stirring and heating, and add 0.5% anhydrous sodium carbonate (by dry weight of silkworm cocoon powder) to the system. Stir at 100 rpm and heat the mixture to 100℃, then reflux and extract at this temperature for 3 hours. After extraction, centrifuge at 4000 rpm for 15 minutes at 40℃, collect the clear liquid to obtain crude sericin extract, and then concentrate at 35℃, -0.09MPa vacuum, and 80 rpm for 80 minutes to obtain dilute sericin aqueous solution. S3. Pump the diluted sericin aqueous solution into the nanofiltration membrane system, use a polyamide nanofiltration membrane, select a molecular weight cutoff of 500 Da, and circulate and concentrate it to a solid content of 15% under the conditions of temperature of 25℃, pressure of 1.2MPa, and transmembrane flow rate of 22L / h to obtain the nanofiltration concentrated sericin protein solution. S4. After the nanofiltration concentrated sericin solution is filtered through a 0.22μm microporous membrane, it is placed in a freeze dryer and freeze-dried at a condenser temperature of -55℃ and a vacuum degree of 8 Pa until the moisture content of the material is less than 5%, and finally white to off-white sericin is obtained and sealed for storage.

[0056] Table 1. Components and weight ratios of the composition Components Sericin European linden flower extract Schisandra chinensis extract Example 1 0.2 0.8 0.2 Example 2 0.2 0.8 0.2 Example 3 0.2 0.8 0.2 Example 4 0.2 0.8 0.2 Example 5 0.2 0.8 0.2 Example 6 0.2 0.8 0.2 Example 7 0.2 0.8 0.2 Example 8 0.2 0.8 0.2 Example 9 0.2 0.8 0.2 Example 10 0.05 0.5 0.1 Example 11 0.5 2 0.6 Example 12 0.01 0.1 0.01 Example 13 1 3 1 Comparative Example 1 0.2 0.8 0.2 Comparative Example 2 0.2 0.8 0.2 Comparative Example 3 0.2 0.8 0.2 Comparative Example 4 0.2 0.8 0.2 Comparative Example 5 0.2 0.8 0.2 Comparative Example 6 0.2 0.8 0.2 Comparative Example 7 0.2 0.8 0.2 Comparative Example 8 0.2 0.8 0.2 Comparative Example 9 0.2 0.8 0.2 Comparative Example 10 / 0.8 0.2 Comparative Example 11 0.2 / 0.2 Comparative Example 12 0.2 0.8 / Comparative Example 13 2 0.1 2 Comparative Example 14 0.01 4 0.01 Note: The total weight of the compositions in the examples and comparative examples in Table 1 is the same.

[0057] Test Example 1: Protein Purity Test The protein content of the sericin samples used in Examples 1-9 and Comparative Examples 1-9 was determined using the Kjeldahl method (GB 5009.5-2025 "National Food Safety Standard - Determination of Protein in Food"). The specific detection method is as follows: Digestion: Accurately weigh approximately 0.2 g of sericin sample (accurate to 0.0001 g) and place it in a 250 mL quantitative flask. Add 0.4 g of copper sulfate, 6 g of potassium sulfate, and 20 mL of concentrated sulfuric acid and heat together for 1 hour. Remove the nitrogen determination flask and cool it to room temperature. Add 20 mL of water and transfer all the contents to a 100 mL volumetric flask. Wash the inner wall of the nitrogen determination flask with a small amount of water and add the washing solution to the volumetric flask. Add water to the mark, mix well, and set aside. At the same time, perform a blank test. Distillation: Assemble the nitrogen distillation apparatus according to GB 5009.5-2025, transfer the digestion liquid to the Kjeldahl nitrogen analyzer, wash the digestion tube several times with 15 mL of water, and transfer the washing liquid into the reaction chamber. Add 40 mL of sodium hydroxide solution (400 g / L) to the reaction chamber, immediately seal the distillation apparatus, and introduce steam to start distillation. After 15 min, move the distillate receiving flask until the liquid level is above the lower end of the condenser, and continue distilling for about 1 min until the distillate is neutral when tested with pH paper. Absorption and titration: Titrate the absorption solution in the receiving flask with a standardized 0.1 mol / L hydrochloric acid solution; Titration endpoint: The solution color changes from bright green to dark purple (or grayish purple) and does not fade within 30 seconds. Record the volume of hydrochloric acid standard solution consumed as V2. The protein content X of each group of samples is calculated using the following formula: ; Wherein, V2 is the volume of hydrochloric acid standard solution consumed by the sample, mL; V1 is the volume of hydrochloric acid standard solution consumed by the blank test, mL; C is the concentration of hydrochloric acid standard solution, mol / L; 0.014 is the millimolecular mass of nitrogen, g / mmol; F is the protein conversion factor, which is 6.25; m is the mass of the sample, g; V3 is the total volume of the sample digestion solution, mL; V4 is the volume of the digestion solution used for distillation, mL; the calculation results are recorded in Table 4.

[0058] Test Example 2: Stimulation Test (1) Test samples: Samples prepared in Examples 1-13 and Comparative Examples 1-14; (2) Test method: According to the "SN / T 2329-2009 Test for Eye Irritation / Corrosivity of Chicken Embryo Chollial Membrane in Cosmetics", 9-day-old chicken embryos were purchased, and defective embryos were inspected and discarded. The air cell location was marked on the surface of the normal chicken embryo eggshell, and the eggshell was peeled off to expose the white egg membrane. An appropriate amount of physiological saline (0.9% sodium chloride solution) was added with a disposable dropper to moisten the egg membrane. The physiological saline was poured out, and the egg membrane was gently peeled off with medical curved forceps, taking care to ensure that the vascular membrane was not damaged. Confirm the integrity of the vascular membrane, select an intact vascular site, gently place a Teflon ring, and add 40ul of test sample; Observe the vascular bleeding for 3 minutes, and repeat 6 chicken embryos for each sample group; use 0.9% sodium chloride saline as negative control and 0.1M sodium hydroxide as positive control; For experiments using the endpoint evaluation method, the endpoint score (ES) should be calculated and the results should be retained to two decimal places: the score for each chicken embryo is the sum of the observed bleeding, coagulation and vascularization in each chicken embryo; the ES is the mathematical sum of the scores of 6 chicken embryos. The eye irritation of the test substance was compared according to Table 2, and the endpoint results were scored according to Table 3. The results are recorded as shown in Table 4.

[0059] Table 2 Irritation Rating Comparison Table

[0060] Table 3 Evaluation of Endpoint Scoring Method Results Stimulus rating Irritant Classification ES≤12 Non-irritating / mildly irritating 12 < ES < 16 moderate irritation ES≥16 Strong irritant Test Example 3: In Vitro Repair Test The scratch assay is an effective way to evaluate the skin barrier repair capacity. By observing cell migration and regeneration after scratching, it can directly reflect the ability of the composition to repair the damaged cellular barrier. In the experiment, by comparing the cell healing speed, the impact of different treatments on skin repair can be assessed.

[0061] The test samples of the compositions from Examples 1-13 and Comparative Examples 1-14 were tested according to the following steps. The cells used were human keratinocytes (HaCaT, Guangzhou Genio Biotechnology Co., Ltd.). The test conditions were: incubator temperature 37±1℃, humidity 90±5%, and carbon dioxide 5±1%. Cells were cultured and treated according to the groups, followed by testing. Specifically, the test used two-well cell healing slides, and the test method is as follows: (1) Sample solution preparation: The compositions in Examples 1-13 and Comparative Examples 1-14 were dissolved in quantitative DMSO and then diluted with DMEM cell culture medium to a final concentration of 0.05%, which was recorded as the sample solution and refrigerated. (2) Resuscitating HaCaT cells: Take out the 6-well plate, use sterile forceps to fix the 2-well wound healing inserts in the middle of the corresponding wells, and seed the cell suspension into the 6-well plate with the 2-well wound healing inserts at a density of 6 × 10⁶ cells per well. 5 Add 1 mL of DMEM high glucose medium (Gibco) containing 10% fetal bovine serum to each well and revive and culture for 24 h; (3) Drug administration: Add 1 mL of DMEM culture medium containing 0.05% of the corresponding sample solution to the sample group, remove the insert, and continue to culture for 48 h; (4) Photographs: The cell scratches of each group were photographed under a microscope at 0h and 48h, and the area of ​​the scratches was measured.

[0062] The skin barrier repair ability of the samples was represented by the cell scratch healing rate, which was calculated as follows, and the results are recorded in Table 4: Cell healing rate (%) = (cell scratch area 0h - cell scratch area 48h) / cell scratch area 0h × 100%.

[0063] Table 4 Test Results of Test Examples 1-3 Group Protein content (g / 100g) Stimulus rating Cell healing rate (%) Example 1 95.212 1 85.6 Example 2 93.503 1 81.2 Example 3 94.861 2 83.4 Example 4 94.554 1 82.8 Example 5 93.943 1 81.9 Example 6 94.731 2 82.3 Example 7 91.110 3 79.5 Example 8 93.845 1 82.0 Example 9 92.576 1 80.4 Example 10 / 1 78.4 Example 11 / 1 75.1 Example 12 / 3 70.9 Example 13 / 2 72.4 Comparative Example 1 86.501 5 47.5 Comparative Example 2 80.853 9 40.5 Comparative Example 3 84.169 7 42.0 Comparative Example 4 85.204 7 45.7 Comparative Example 5 84.935 8 43.5 Comparative Example 6 78.013 9 38.0 Comparative Example 7 76.155 9 35.0 Comparative Example 8 70.820 11 30.4 Comparative Example 9 72.479 12 31.5 Comparative Example 10 / 3 22.3 Comparative Example 11 / 4 20.2 Comparative Example 12 / 4 25.1 Comparative Example 13 / 5 53.7 Comparative Example 14 / 5 49.6 As shown in Table 3, compared with Comparative Examples 1-14, the repairing and soothing compositions provided in Examples 1-13 can effectively improve cell healing rate, thereby enhancing the skin's barrier repair ability. Furthermore, these compositions are gentle and non-irritating, making them suitable for people with sensitive skin. Figure 1 The cell healing status of Example 1 and Comparative Example 2 at 0h and 48h is shown. Figure 2 The diagram illustrates the irritation levels of Example 1 and Comparative Example 2. As can be seen, the composition of Example 1 is less irritating and has a better repair-promoting effect compared to Comparative Example 2.

[0064] Comparative Examples 1-9 and 1-7 show that the selection of key parameters in the extraction and purification of sericin using supercritical CO2 treatment significantly affects the protein content of the obtained sericin, thereby influencing its activity and the composition's repair efficacy. Comparative Example 8 and Example 1 show that supercritical CO2 pretreatment significantly improves the extraction purity of sericin, possibly because this treatment activates the silkworm cocoon matrix, facilitating more complete dissolution of the sericin. Furthermore, the supercritical CO2 extraction and purification method is significantly superior to the hot water extraction in Comparative Example 9. In addition, the experimental results of Comparative Examples 1 and 1-13 indicate that the sericin obtained by the specific process in this invention exhibits a synergistic relationship with European linden flower extract and Schisandra chinensis extract. Moreover, when the weight ratio of sericin, European linden flower extract, and Schisandra chinensis extract is (0.01-1):(0.1-3):(0.01-1), the composition demonstrates better barrier repair ability.

[0065] Application Example 1-13, Comparison Application Example 1-14 and Blank Application Example The repairing and soothing compositions of Examples 1-13 and Comparative Examples 1-14 were added to the serum at a concentration of 3 wt% to obtain the serums of Application Examples 1-13 and Comparative Application Examples 1-14, respectively. In addition, blank application examples without the addition of the repairing and soothing compositions were prepared. The serum formulations are shown in Table 5.

[0066] The preparation method of the serum specifically includes the following steps: S1. Mix the humectant, thickener and deionized water, heat to 85°C, and homogenize at 1300 rpm for 4 minutes. After homogenization, keep warm for later use to obtain the pre-prepared phase A. S2. Mix the preservatives and heat to 60°C to melt them, to obtain the pre-prepared phase B. S3. After cooling the pre-prepared phase A to 60°C, add the pre-prepared phase B at 300 rpm and stir until well mixed. Cool to 45°C, add the repair and soothing composition, and continue stirring at 300 rpm for 8 minutes. Add the pH adjuster, stir until uniform, and discharge to obtain the essence.

[0067] Table 5 Serum Formula

[0068] Effectiveness test: Soothing redness and improving skin barrier function in sensitive skin. Volunteers were enrolled according to the general principles of safety testing in the *Cosmetic Safety Technical Specifications*. The trial selected 84 Asian adults aged 18-60 with self-reported skin sensitivity and a lactic acid stinging score ≥3. They were randomly divided into 28 groups of 3 participants each. On the day of their visit, volunteers washed their faces with water in the test area without applying any products and sat quietly for 20 minutes in an air-conditioned room with a temperature of 21±1℃ and humidity of 50±10%. The transepidermal water loss (TEWL) of the cheekbone and the a* value of facial redness were measured using the Tewameter™ Hex probe and the Colorimeter CL440 skin color probe. Testing timeline: Participants used the serum twice daily, morning and evening, approximately 1g per pump each time. A follow-up visit was conducted after 28 days to test the TEWL of the cheekbone and the degree of facial redness.

[0069] Improvement rate of each skin parameter before and after use = (mean before use - mean after use) / mean before use × 100%; The improvement effect of the application examples on the barrier is represented by the TEWL improvement rate, and the improvement effect of the application examples on redness is represented by the a* value improvement rate. The calculation results are shown in Table 6.

[0070] Table 6 Results of the Effect Test Group TEWL improvement rate / % Erythema improvement rate / % Blank application example 2.7 4.1 Application Example 1 27.1 32.5 Application Example 2 24.8 29.4 Application Example 3 26.2 31.2 Application Example 4 26.0 30.8 Application Example 5 25.1 29.7 Application Example 6 25.8 30.5 Application Example 7 23.8 28.2 Application Example 8 25.6 30.2 Application Example 9 24.5 28.6 Application Example 10 23.4 27.8 Application Example 11 22.0 26.5 Application Example 12 19.7 24.0 Application Example 13 20.5 25.1 Comparative Application Example 1 9.8 13.4 Comparative Application Example 2 8.7 12.3 Comparative Application Example 3 9.0 12.5 Comparative Application Example 4 9.2 12.7 Comparative Application Example 5 9.4 13.1 Comparative Application Example 6 8.3 11.8 Comparative Application Example 7 8.2 11.5 Comparative Application Example 8 7.9 11.2 Comparative Application Example 9 7.3 10.7 Comparative Application Example 10 5.2 7.9 Comparative Application Example 11 4.6 6.5 Comparative Application Example 12 5.7 8.3 Comparative Application Example 13 10.9 14.5 Comparative Application Example 14 10.3 13.8 As shown in Table 6, compared with Comparative Application Examples 1-14, the repairing and soothing compositions provided in Application Examples 1-13 have higher TEWL improvement rates and erythema improvement rates, and can effectively soothe and improve facial redness.

[0071] Among them, the improvement rates of TEWL and erythema in Application Example 1 and Comparative Application Examples 10-14 show that the sericin, European linden flower extract, and Schisandra chinensis extract of the present invention have a synergistic effect. Moreover, when the weight ratio of sericin, European linden flower extract, and Schisandra chinensis extract is (0.01-1):(0.1-3):(0.01-1), the composition has a better effect on improving TEWL and erythema. The preparation of sericin in Application Examples 1-9 and Comparative Application Examples 1-9 shows that the preparation of sericin in the present invention has its own characteristics. In summary, the selection of components and the preparation of sericin in the present invention are irreplaceable. Any substitution of the components or change of the preparation method of sericin may result in the final repair and soothing effect failing to meet the expectations of the present invention.

[0072] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any indirect modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A repairing and soothing composition suitable for sensitive skin, characterized in that, The composition comprises the following components: sericin, European linden flower extract and Schisandra chinensis extract, wherein the weight ratio of sericin, European linden flower extract and Schisandra chinensis extract is (0.01-1):(0.1-3):(0.01-1).

2. The repairing and soothing composition suitable for sensitive skin according to claim 1, characterized in that, The weight ratio of the sericin, European linden flower extract, and Schisandra chinensis extract is (0.05-0.5):(0.5-2):(0.1-0.6).

3. The repairing and soothing composition suitable for sensitive skin according to claim 1, characterized in that, The sericin was obtained by extraction and purification after supercritical CO2 treatment, specifically including the following steps: S1. After washing the silkworm cocoons with pure water, use an ethanol solution for preliminary degreasing, then dry the degreased cocoon material, pulverize it through a 40-80 mesh sieve to obtain silkworm cocoon powder. S2. Load the silkworm cocoon powder into the extraction vessel with a filling coefficient of 60%-75%, close the extraction vessel, introduce supercritical CO2 fluid, start the high-pressure pump to pressurize, and heat the system to the set temperature. After the system stabilizes, pump in the entrainer for dynamic circulation treatment to obtain the treated fluid mixture. S3. The treated fluid mixture is sequentially introduced into the primary separation vessel and the secondary separation vessel. The entrained agent is separated and recovered by depressurization and heating. The CO2 is condensed and recycled to obtain silkworm cocoon solid material that has been deeply degreased and activated. S4. The silkworm cocoon solid material that has been deeply degreased and activated is stirred and extracted with the extraction solvent under mild conditions to dissolve the sericin. Then, the solid and liquid are separated to obtain a crude sericin extract. The crude extract is transferred to a vacuum rotary evaporator for concentration to obtain a dilute sericin aqueous solution. S5. Pump the dilute sericin aqueous solution into the nanofiltration membrane system for concentration and dialysis at room temperature to obtain nanofiltration concentrated sericin protein solution. S6. The nanofiltration concentrated sericin solution is filtered through a 0.22μm microporous membrane and then freeze-dried to obtain sericin.

4. The repairing and soothing composition suitable for sensitive skin according to claim 3, characterized in that, In step S1, the ratio of silkworm cocoons to ethanol solution is 1g:10-30mL, the concentration of ethanol solution is 60-70% (v / v), the preliminary degreasing is carried out by stirring at 25℃-35℃ for 0.5-1.5h, and the drying is carried out at 35℃-45℃ and vacuum degree below -0.09MPa for 12-36h until the moisture content of the raw material is below 5%.

5. The repairing and soothing composition suitable for sensitive skin according to claim 3, characterized in that, The supercritical CO2 treatment in step S2 is carried out under the conditions of pressure 25MPa-45MPa, 35℃-45℃, and CO2 flow rate of 15-35 L / h. The entrainer is a 20%-50% (v / v) ethanol solution, and the flow rate-to-mass ratio of the entrainer to supercritical CO2 is 1:(15-40). The dynamic circulation extraction time is 1.5-4h.

6. The repairing and soothing composition suitable for sensitive skin according to claim 3, characterized in that, In step S3, the separation temperature and pressure of the primary separation vessel are 40℃-50℃ and 6MPa-15MPa, respectively, and the separation temperature and pressure of the secondary separation vessel are 35℃-45℃ and 4MPa-7MPa, respectively.

7. The repairing and soothing composition suitable for sensitive skin according to claim 3, characterized in that, In step S4, the ratio of the deep-degreased and activated silkworm cocoon solid material to the extraction solvent is 1 g: 15-25 mL. The extraction solvent is deionized water. The extraction is carried out at 40-50℃ and 50-150 rpm for 2-4 h with stirring. The concentration is carried out at 30℃-40℃, -0.08MPa to -0.1MPa vacuum, and 60-100 rpm for 40-90 min.

8. The repairing and soothing composition suitable for sensitive skin according to claim 3, characterized in that, The room temperature concentration and dialysis described in step S5 involves using a nanofiltration membrane made of polyamide, polypiperazine amide, or sulfonated polyethersulfone, with a molecular weight cutoff of 200 Da-500 Da, and cyclically concentrating the membrane to a solid content of 10%-20% under the conditions of a temperature of 15℃-35℃, a pressure of 0.8MPa-2.0MPa, and a transmembrane flow rate of 15 L / h-30 L / h.

9. The use of the repairing and soothing composition suitable for sensitive skin according to any one of claims 1-8 in a skin care product, characterized in that, The skincare products include toners, lotions, creams, masks, serums, and sprays, and the amount of the composition added is 0.5%-3% of the total weight of the skincare products.

10. An essence, characterized in that, The serum comprises the following ingredients by weight percentage: 0.5%-3% of the repairing and soothing composition suitable for sensitive skin according to any one of claims 1-8, 0.05%-0.3% of thickener, 0.5%-10% of moisturizer, 0.5%-5% of preservative, 0.01%-0.3% of pH adjuster, and the balance being deionized water.

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