Stem cell gel preparation system and process

Abstract

The stem cell gel preparation system comprises a preparation tank body, a supporting seat and a sealing cover body, the bottom of the preparation tank body is fixedly connected with the supporting seat, the bottom of the supporting seat is provided with supporting legs, and the top of the preparation tank body is provided with the sealing cover body; the driving motor is arranged at the top of the sealing cover body, and a liquid inlet pipe is arranged on the left side of the driving motor. According to the stem cell gel preparation system and process, a plurality of rotating gears on the outer side and the mixing shafts are driven to rotate in the preparation tank through rotation of a transmission gear, stirring blades are arranged on the mixing shafts, and liquid in the preparation tank is uniformly mixed and stirred through simultaneous rotation of the mixing shafts and the stirring blades, so that the liquid is rapidly and uniformly mixed; the ultrasonic generator is arranged in the mixing shaft, and ultrasonic waves generated by the ultrasonic generator are used for dispersing and mixing liquid, so that the liquid is mixed more uniformly.

Description

Technical Field

[0001] This invention relates to the field of stem cell gel preparation technology, specifically to a stem cell gel preparation system and process. Background Technology

[0002] Wound healing is a complex process involving reepithelialization of the skin defect, proliferation of granulation tissue, inflammatory response, angiogenesis, and wound remodeling. Problems in any of these processes can lead to impaired wound healing, inhibiting the differentiation and paracrine function of mesenchymal stem cells, resulting in slow epidermal regeneration, reduced inflammatory response, and decreased wound resistance to infection. Even for small wounds, gels containing recombinant human PDGF and other cytokines can accelerate wound healing, whether used alone or in combination, for superficial second- to third-degree burns, varicose ulcers of the lower extremities, diabetic foot, radiation ulcers, and pressure ulcers.

[0003] Stem cell supernatant gel is a type of stem cell gel. In the preparation of stem cell supernatant gel, a stirring device is used to mix the liquid evenly. Nowadays, stirring and mixing mostly adopts regular stirring in the same direction. Without proper random stirring, it takes a certain amount of time to make the liquid evenly mixed, which reduces the preparation efficiency of stem cell supernatant gel to some extent.

[0004] In view of the existing problems, there is an urgent need in the field to innovate on the basis of the original stem cell gel preparation. Therefore, the technical solution of the present invention is proposed. Summary of the Invention

[0005] The purpose of this invention is to provide a stem cell gel preparation system and process to address the issue mentioned in the background art that stem cell supernatant gel is a type of stem cell gel. In the preparation process of stem cell supernatant gel, a stirring device is used to uniformly mix the liquid. Nowadays, stirring and mixing mostly adopts regular stirring in the same direction, which does not effectively perform random mixing and requires a certain amount of time to achieve uniform mixing of the liquid, thus reducing the preparation efficiency of stem cell supernatant gel to some extent.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a stem cell gel preparation system, comprising a preparation tank, a support base, and a sealing cap, wherein the bottom of the preparation tank is fixedly connected to the support base, and the bottom of the support base is provided with support feet, and the top of the preparation tank is provided with a sealing cap;

[0007] Also includes:

[0008] A drive motor is located on the top of the sealing cover, and an inlet pipe is provided on the left side of the drive motor, and an outlet pipe is provided on the right side of the drive motor, with a feed pipe provided on the side of the outlet pipe.

[0009] A rotating shaft is located at the output end of the drive motor, and a transmission gear is provided at the end of the rotating shaft, and the transmission gear is meshed with a rotating gear on its side.

[0010] A mixing mechanism is installed at the bottom of the rotating gear, and the mixing mechanism includes a mixing shaft and a liquid storage cavity. A stirring blade is fixedly connected to the outer wall of the mixing shaft, and a liquid storage cavity is opened inside the mixing shaft.

[0011] The flow diversion mechanism is located outside the rotating shaft, and the flow diversion mechanism includes a flow diversion housing and a flow groove, and the flow diversion housing has a flow groove inside.

[0012] As an optional embodiment of the stem cell gel preparation system of the present invention, the inlet pipe is connected to the outlet pipe through a diversion shell and a flow channel, and the diversion shell is arranged in a circular ring shape. A fixing frame is provided on the outer wall of the diversion shell, and the fixing frame and the sealing cover are welded together.

[0013] As an optional embodiment of the stem cell gel preparation system of the present invention, the transmission gear and the sealing cover are movably connected, the diameter of the transmission gear is larger than the diameter of the rotating gear, and the rotating gear is set at an equal angle with respect to the center of the transmission gear in a top view.

[0014] As an optional embodiment of the stem cell gel preparation system of the present invention, the rotating gear and the mixing mechanism are arranged in a one-to-one correspondence. The mixing mechanism further includes a heat exchange plate and an ultrasonic generator. The rotating gear and the mixing shaft are fixedly connected, and the inner wall of the mixing shaft is provided with a heat exchange plate.

[0015] As an optional embodiment of the stem cell gel preparation system of the present invention, an ultrasonic generator is provided on the side of the heat exchange plate, and the heat exchange plate is arranged at an equal angle with respect to the ultrasonic generator, and a connecting line is provided at the end of the ultrasonic generator.

[0016] As an optional embodiment of the stem cell gel preparation system of the present invention, a connecting tube is provided on the outside of the connecting line, and the connecting tube is fixedly connected to the mixing shaft, and the connecting tube is interconnected with the liquid storage cavity.

[0017] As an optional embodiment of the stem cell gel preparation system of the present invention, wherein: the end of the connecting line is electrically connected to a pressure plate, and a current collector ring is attached to the outer side of the pressure plate, and a support frame is provided on the outer wall of the current collector ring, and the support frame is fixedly connected to the sealing cover.

[0018] As an optional embodiment of the stem cell gel preparation system of the present invention, the outer wall of the connecting tube is provided with a connecting tube, and a limiting plate is fixedly connected to the outer wall of the connecting tube. A guide tube is provided on the outer side of the limiting plate, and a sealing packing is provided on the inner side of the connecting tube.

[0019] As an optional embodiment of the stem cell gel preparation system of the present invention, the diversion mechanism further includes a first blocking plate and a second blocking plate, both of which are fixedly disposed on the inner wall of the diversion shell, and both are arranged at equal angles, while the first blocking plate and the second blocking plate are staggered.

[0020] This invention also discloses a process for preparing stem cell gel, comprising the following steps:

[0021] S1: First, mesenchymal stem cells are cultured in serum-free stem cell culture medium, and the supernatant is collected;

[0022] S2: Add ammonium sulfate to the supernatant and feed it into the preparation tank through the feed pipe. Control the rotation of the mixing shaft and the stirring blade to mix and stir the liquid in the preparation tank.

[0023] S3: Then the liquid in the preparation tank is taken out and left to stand, centrifuged, the precipitate is taken, dissolved in water, filtered, and the protein filtrate is obtained. The purified supernatant is then freeze-dried to obtain the freeze-dried supernatant.

[0024] S4: Dissolve the freeze-dried supernatant in sterile water, and mix the aqueous solution with an equal volume of methylcellulose aqueous solution to obtain stem cell supernatant gel.

[0025] Compared with the prior art, the beneficial effects of the present invention are:

[0026] 1. The stem cell gel preparation system and process are equipped with a mixing shaft. The rotation of the transmission gear drives several rotating gears and the mixing shaft on the outside to rotate inside the preparation tank. The mixing shaft is equipped with a stirring blade. The simultaneous rotation of multiple mixing shafts and stirring blades is used to uniformly mix the liquid in the preparation tank, allowing it to be mixed quickly and evenly. In addition, an ultrasonic generator is installed inside the mixing shaft. The ultrasonic waves generated by the ultrasonic generator are used to disperse and mix the liquid, making the mixing more uniform and effectively improving its processing efficiency.

[0027] 2. The stem cell gel preparation system and process are equipped with a current collector ring. When the mixing shaft rotates, the pressure plate will rotate within the current collector ring. The two are electrically connected, so that when the mixing shaft drives the ultrasonic generator to rotate, the ultrasonic generator is always in working condition to uniformly mix the materials inside the preparation tank.

[0028] 3. The stem cell gel preparation system and process are equipped with a liquid storage cavity. Utilizing the liquid storage cavity inside the mixing shaft, the liquid in the distribution shell can enter the interior of the liquid storage cavity through the guide pipe and connecting pipe. The mixing shaft is also equipped with multiple heat exchange plates to increase the contact surface with the liquid, keeping the mixing shaft at a low temperature. This prevents the mixing shaft temperature from being too high during stirring, which could affect the activity of the substances and cells. Furthermore, the liquid flowing inside the liquid storage cavity carries away the heat generated by the ultrasonic generator, keeping it in a low-temperature operating state. The ultrasonic generator also facilitates convective exchange between the liquid in the liquid storage cavity and the liquid in the distribution shell, ensuring that the liquid in the liquid storage cavity remains at a low temperature. Attached Figure Description

[0029] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0030] Figure 2 This is a schematic diagram of the connection structure between the sealing cover and the mixing mechanism of the present invention;

[0031] Figure 3 This is a schematic cross-sectional view of the sealing cover structure of the present invention;

[0032] Figure 4 This is a schematic diagram of the connection structure between the transmission gear and the rotating gear of the present invention;

[0033] Figure 5 This is a schematic diagram of the connection structure between the flow divider housing and the first baffle plate of the present invention;

[0034] Figure 6 This is a schematic cross-sectional view of the mixing mechanism of the present invention;

[0035] Figure 7 This is a schematic diagram of the connection structure between the rotating gear and the mixing shaft of the present invention;

[0036] Figure 8 For the present invention Figure 7 Enlarged structural diagram at point A in the middle;

[0037] Figure 9 This is a schematic diagram of the connection structure between the mixing shaft and the heat exchange plate of the present invention.

[0038] In the diagram: 1. Preparation tank; 2. Support base; 3. Support foot; 4. Sealing cover; 5. Feed pipe; 6. Drive motor; 7. Liquid inlet pipe; 8. Rotating shaft; 9. Transmission gear; 10. Rotating gear; 11. Mixing mechanism; 1101. Mixing shaft; 1102. Liquid storage cavity; 1103. Heat exchange plate; 1104. Ultrasonic generator; 12. Stirring plate; 13. Connecting line; 14. Connecting pipe; 15. Pressure plate; 16. Slip ring; 17. Support frame; 18. Connecting pipe; 19. Limiting plate; 20. Sealing packing; 21. Guide pipe; 22. Diverting mechanism; 2201. Diverting shell; 2202. Flow channel; 2203. First blocking plate; 2204. Second blocking plate; 23. Fixing frame; 24. Drain pipe. Detailed Implementation

[0039] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0040] Example 1

[0041] This embodiment aims to facilitate the solution to the problem of how to achieve rapid and uniform mixing of liquids. Please refer to [link / reference]. Figures 1 to 6 The present invention provides a technical solution: a stem cell gel preparation system, including a preparation tank 1, a support base 2 and a sealing cover 4. The bottom of the preparation tank 1 is fixedly connected to the support base 2, and the bottom of the support base 2 is provided with support feet 3. The top of the preparation tank 1 is provided with a sealing cover 4.

[0042] Also includes:

[0043] A drive motor 6 is located on the top of the sealing cover 4, and an inlet pipe 7 is provided on the left side of the drive motor 6, and a drain pipe 24 is provided on the right side of the drive motor 6, and a feed pipe 5 is provided on the side of the drain pipe 24.

[0044] A rotating shaft 8 is located at the output end of the drive motor 6, and a transmission gear 9 is provided at the end of the rotating shaft 8, and a rotating gear 10 is meshed with the side of the transmission gear 9.

[0045] The mixing mechanism 11 is installed at the bottom of the rotating gear 10, and the mixing mechanism 11 includes a mixing shaft 1101 and a liquid storage cavity 1102. The outer wall of the mixing shaft 1101 is fixedly connected with a stirring blade 12, and the liquid storage cavity 1102 is opened inside the mixing shaft 1101.

[0046] The diversion mechanism 22 is located outside the rotating shaft 8, and the diversion mechanism 22 includes a diversion housing 2201 and a flow groove 2202, and the flow groove 2202 is provided inside the diversion housing 2201.

[0047] The transmission gear 9 is movably connected to the sealing cover 4, and the diameter of the transmission gear 9 is larger than the diameter of the rotating gear 10. The rotating gear 10 is set at an equal angle with respect to the center of the transmission gear 9 in a top view. The rotating gear 10 is set in a one-to-one correspondence with the mixing mechanism 11. The mixing mechanism 11 also includes heat exchange plates 1103 and an ultrasonic generator 1104. The rotating gear 10 is fixedly connected to the mixing shaft 1101, and the inner wall of the mixing shaft 1101 is provided with heat exchange plates 1103.

[0048] First, the liquid to be mixed is added into the preparation tank 1 through the feed pipe 5 on the sealing cover 4. The drive motor 6 on the sealing cover 4 is then rotated, causing the rotating shaft 8 and transmission gear 9 to rotate within the sealing cover 4. A rotating gear 10 is positioned at equal angles on the outer side of the transmission gear 9, and the transmission gear 9 and rotating gear 10 are meshed together. The rotation of the transmission gear 9 drives the outer rotating gear 10 to rotate simultaneously. A mixing shaft 1101 is positioned at the bottom of the rotating gear 10, and stirring blades are positioned at equal angles on the outer wall of the mixing shaft 1101. 12. Multiple mixing shafts 1101 and stirring blades 12 are used to randomly mix and stir the liquid, improving the liquid mixing processing efficiency. An ultrasonic generator 1104 is installed inside the mixing shaft 1101. The sound waves of the ultrasonic generator 1104 are used to disperse and mix the liquid, making the liquid mixture more uniform and further improving its processing efficiency. When it is necessary to clean the inside of the preparation tank 1 later, clean water is injected, and the mixing shaft 1101 and ultrasonic generator 1104 are used to quickly clean the inside, improving its internal cleaning effect.

[0049] Example 2

[0050] This embodiment aims to facilitate a solution to the problem of how to keep the mixing shaft 1101 and the ultrasonic generator 1104 at a low temperature. This embodiment is an improvement on Embodiment 1. For details, please refer to [link to Embodiment 1]. Figures 2 to 9The inlet pipe 7 is connected to the outlet pipe 24 through the diversion shell 2201 and the flow channel 2202. The diversion shell 2201 is arranged in a circular structure. The outer wall of the diversion shell 2201 is provided with a fixing frame 23. The fixing frame 23 is welded to the sealing cover 4. An ultrasonic generator 1104 is provided on the side of the heat exchange plate 1103. The heat exchange plate 1103 is arranged at an equal angle with respect to the ultrasonic generator 1104. A connecting line 13 is provided at the end of the ultrasonic generator 1104. A connecting pipe 14 is provided on the outside of the connecting line 13. The connecting pipe 14 is fixedly connected to the mixing shaft 1101. The connecting pipe 14 is connected to the liquid storage cavity 1102.

[0051] The outer wall of the connecting pipe 14 is provided with a connecting pipe 18, and a limiting piece 19 is fixedly connected to the outer wall of the connecting pipe 18. A guide pipe 21 is provided on the outer side of the limiting piece 19, and a sealing packing 20 is provided on the inner side of the connecting pipe 18. The diversion mechanism 22 also includes a first blocking piece 2203 and a second blocking piece 2204. The first blocking piece 2203 and the second blocking piece 2204 are both fixedly provided on the inner wall of the diversion housing 2201. The first blocking piece 2203 and the second blocking piece 2204 are both arranged at equal angles and are staggered.

[0052] During the mixing process, cooling water is injected into the inlet pipe 7, allowing it to flow into the interior of the distribution housing 2201. The bottom of the distribution housing 2201 is connected to a guide pipe 21, allowing the liquid to pass through the guide pipe 21, the connecting pipe 18, and the connecting pipe 14, and finally enter the liquid storage cavity 1102 inside the mixing shaft 1101. The mixing shaft 1101 is equipped with several heat exchange plates 1103 to increase the contact surface with the coolant, keeping the mixing shaft 1101 at a low temperature. This prevents the temperature generated during the mixing process from affecting the substances and cell activity in the liquid. The coolant inside the liquid storage cavity 1102 also comes into contact with the ultrasonic generator 1104 to cool down the temperature generated by the ultrasonic generator 1104 during operation, keeping it at a low temperature and preventing overheating from affecting its service life.

[0053] Furthermore, the interior of the diversion housing 2201 is provided with a number of first blocking plates 2203 and second blocking plates 2204. The first blocking plates 2203 and second blocking plates 2204 are arranged alternately. The multiple first blocking plates 2203 and second blocking plates 2204 are used to block the cooling water. At this time, the ultrasonic generator 1104 installed inside the mixing shaft 1101 generates ultrasonic waves during normal operation, which causes the coolant in the liquid storage cavity 1102 and the liquid inside the diversion housing 2201 to flow relative to each other. This facilitates heat exchange between the hotter coolant in the liquid storage cavity 1102 and the colder coolant in the diversion housing 2201, and helps to keep the mixing shaft 1101 and the ultrasonic generator 1104 at a low temperature during operation.

[0054] Furthermore, a limiting piece 19 is provided on the outer wall of the connecting pipe 18. The limiting piece 19 is arranged in a circular shape and embedded inside the guide pipe 21 to limit the docking of the connecting pipe 18 and the guide pipe 21. At the same time, it allows the connecting pipe 18 to rotate normally inside the guide pipe 21, so as not to affect the rotation of the subsequent mixing shaft 1101. In addition, a sealing packing 20 is provided between the connecting pipe 18 and the guide pipe 21 to reduce the connection gap between the two and prevent the coolant from flowing out from the gap between them.

[0055] Example 3

[0056] This embodiment aims to facilitate the solution to the problem of how to ensure normal operation of the ultrasonic generator 1104 when it is rotating. This embodiment is an improvement based on Embodiment 1. For details, please refer to [link / reference]. Figures 6 to 8 The end of the connecting wire 13 is electrically connected to a pressure plate 15, and a current collector ring 16 is attached to the outside of the pressure plate 15. A support frame 17 is provided on the outer wall of the current collector ring 16, and the support frame 17 is fixedly connected to the sealing cover 4.

[0057] The ultrasonic generator 1104 is provided with a connecting line 13 at its end, and a pressure plate 15 is connected to the end of the connecting line 13. When the mixing shaft 1101 rotates, it will simultaneously drive the pressure plate 15 to rotate within the collector ring 16. The contact point between the pressure plate 15 and the collector ring 16 is electrically connected, while other parts are insulated. This ensures that the ultrasonic generator 1104 is always energized when the mixing shaft 1101 drives the ultrasonic generator 1104 to rotate, allowing the ultrasonic generator 1104 to work normally during rotation.

[0058] Example 4

[0059] A process for preparing stem cell gel includes the following steps:

[0060] S1: First, mesenchymal stem cells are cultured in serum-free stem cell culture medium, and the supernatant is collected;

[0061] S2: Add ammonium sulfate to the supernatant and feed it into the preparation tank 1 through the feed pipe 5. Control the mixing shaft 1101 and the stirring plate 12 to rotate to mix and stir the liquid in the preparation tank 1.

[0062] S3: Then, the liquid in preparation tank 1 is taken out and left to stand, centrifuged, the precipitate is taken out, dissolved in water, filtered, and the protein filtrate is obtained. The purified supernatant is then freeze-dried to obtain the freeze-dried supernatant.

[0063] S4: Dissolve the freeze-dried supernatant in sterile water, and mix the aqueous solution with an equal volume of methylcellulose aqueous solution to obtain stem cell supernatant gel.

[0064] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0065] The contents not described in detail in this specification are existing technologies known to those skilled in the art.

[0066] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A stem cell gel preparation system, characterized in that: The preparation tank includes a preparation tank (1), a support base (2) and a sealing cover (4). The bottom of the preparation tank (1) is fixedly connected to the support base (2), and the bottom of the support base (2) is provided with a support foot (3). The top of the preparation tank (1) is provided with a sealing cover (4). A drive motor (6) is located on the top of the sealing cover (4), and an inlet pipe (7) is provided on the left side of the drive motor (6), and a drain pipe (24) is provided on the right side of the drive motor (6), and a feed pipe (5) is provided on the side of the drain pipe (24). A rotating shaft (8) is provided at the output end of the drive motor (6), and a transmission gear (9) is provided at the end of the rotating shaft (8), and a rotating gear (10) is meshed with the side of the transmission gear (9). A mixing mechanism (11) is installed at the bottom of the rotating gear (10), and the mixing mechanism (11) includes a mixing shaft (1101) and a liquid storage cavity (1102). A stirring blade (12) is fixedly connected to the outer wall of the mixing shaft (1101), and a liquid storage cavity (1102) is opened inside the mixing shaft (1101). The diversion mechanism (22) is located outside the rotating shaft (8), and the diversion mechanism (22) includes a diversion housing (2201) and a flow groove (2202), and the flow groove (2202) is provided inside the diversion housing (2201).

2. The stem cell gel preparation system according to claim 1, characterized in that: The inlet pipe (7) is connected to the outlet pipe (24) through the diversion shell (2201) and the flow channel (2202). The diversion shell (2201) is arranged in a circular structure. The outer wall of the diversion shell (2201) is provided with a fixing frame (23), and the fixing frame (23) is welded to the sealing cover (4).

3. A stem cell gel preparation system according to claim 1 or 2, characterized in that: The transmission gear (9) and the sealing cover (4) are movably connected, and the diameter of the transmission gear (9) is larger than the diameter of the rotating gear (10), and the rotating gear (10) is set at an equal angle with respect to the center of the transmission gear (9) in plan view.

4. A stem cell gel preparation system according to any one of claims 1-3, characterized in that: The rotating gear (10) and the mixing mechanism (11) are arranged in a one-to-one correspondence. The mixing mechanism (11) also includes a heat exchange plate (1103) and an ultrasonic generator (1104). The rotating gear (10) and the mixing shaft (1101) are fixedly connected, and the inner wall of the mixing shaft (1101) is provided with heat exchange plates (1103).

5. A stem cell gel preparation system according to any one of claims 1-4, characterized in that: An ultrasonic generator (1104) is provided on the side of the heat exchange plate (1103), and the heat exchange plate (1103) is arranged at an equal angle with respect to the ultrasonic generator (1104), and a connecting line (13) is provided at the end of the ultrasonic generator (1104).

6. The stem cell gel preparation system according to claim 5, characterized in that: A connecting pipe (14) is provided on the outside of the connecting line (13), and the connecting pipe (14) is fixedly connected to the mixing shaft (1101), and the connecting pipe (14) is interconnected with the liquid storage cavity (1102).

7. The stem cell gel preparation system according to claim 6, characterized in that: The end of the connecting line (13) is electrically connected to a pressure plate (15), and a collector ring (16) is attached to the outside of the pressure plate (15). A support frame (17) is provided on the outer wall of the collector ring (16), and the support frame (17) is fixedly connected to the sealing cover (4).

8. The stem cell gel preparation system according to claim 7, characterized in that: The outer wall of the connecting pipe (14) is provided with a connecting pipe (18), and a limiting piece (19) is fixedly connected to the outer wall of the connecting pipe (18). A guide pipe (21) is provided on the outer side of the limiting piece (19), and a sealing packing (20) is provided on the inner side of the connecting pipe (18).

9. The stem cell gel preparation system according to claim 8, characterized in that: The diversion mechanism (22) further includes a first blocking plate (2203) and a second blocking plate (2204), and the first blocking plate (2203) and the second blocking plate (2204) are both fixedly disposed on the inner wall of the diversion housing (2201), and the first blocking plate (2203) and the second blocking plate (2204) are both arranged at equal angles, while the first blocking plate (2203) and the second blocking plate (2204) are arranged in an alternating manner.

10. A process for preparing stem cell gel, characterized in that, The stem cell gel preparation system as described in claims 1-9 includes the following steps: S1: First, mesenchymal stem cells are cultured in serum-free stem cell culture medium, and the supernatant is collected; S2: Add ammonium sulfate to the supernatant and add it into the preparation tank (1) through the feed pipe (5). Control the mixing shaft (1101) and the stirring plate (12) to rotate to mix and stir the liquid in the preparation tank (1). S3: Then the liquid in the preparation tank (1) was taken out and allowed to stand, centrifuged, the precipitate was taken, dissolved in water, filtered, and the protein filtrate was obtained. The purified supernatant was then freeze-dried to obtain the freeze-dried supernatant. S4: Dissolve the freeze-dried supernatant in sterile water, and mix the aqueous solution with an equal volume of methylcellulose aqueous solution to obtain stem cell supernatant gel.

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