Rotary isolation device for cell culture

Abstract

The invention relates to the technical field of cell culture, in particular to a cell culture rotary isolation device which comprises a device shell, a water bath box is detachably mounted in the device shell, a mounting assembly is arranged in the water bath box, and a rotary assembly is arranged between the device shell and the water bath box; in the clamping mechanism, a rotating seat is rotationally connected with a movable block, a clamping seat is rotationally connected with the rotating seat, and the rotating axes of the rotating seat and the clamping seat are coplanar and perpendicularly crossed, so that the inclination angle of the culture dish is adjusted, cells in the culture dish are uniformly stressed in a rotating manner, and the culture quality is improved; the supporting mechanism enables a supporting seat to be tightly attached to the bottom of the culture dish all the time through elastic restoring force of a spring, elastic supporting is achieved, a spherical rotating block and a connecting sleeve are rotationally matched, the supporting seat can synchronously rotate along with angle adjustment of the culture dish, dynamic adaptive supporting is achieved, and damage to the culture dish caused by rigid supporting is avoided; meanwhile, the culture dish is effectively prevented from inclining and turning over in the rotating process, and culture safety is guaranteed.

Description

Technical Field

[0001] This invention relates to the field of cell culture technology, specifically to a cell culture rotating isolation device. Background Technology

[0002] Cell culture devices are core professional equipment in the field of cell culture. Their oscillation culture function can drive the cell culture medium in the culture container to achieve continuous mixing and circulation, so that the cells can fully contact nutrients and oxygen, while efficiently removing metabolic waste. This simulates the physiological microenvironment in cells and provides suitable conditions for cell proliferation and growth.

[0003] In cell culture, dynamic suspension culture is a key method to ensure uniform cell growth and avoid local environmental imbalance. Rotary isolation devices are widely used in cell proliferation and differentiation scenarios because they can simulate dynamic physiological environments. However, most existing cell culture rotating devices use a single rotating structure, which cannot adjust the angle and can only achieve rotational shaking in one direction. This fails to meet the diverse needs of different cell types for culture posture, leading to uneven cell stress, affecting culture quality, and potentially causing localized cell precipitation, resulting in hypoxia and uneven nutrient distribution, severely impacting cell culture outcomes. Summary of the Invention

[0004] The purpose of this invention is to provide a cell culture rotation isolation device to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a cell culture rotating isolation device, comprising a device housing, a water bath tank detachably installed inside the device housing, an installation assembly disposed inside the water bath tank, a rotating assembly disposed between the device housing and the water bath tank, the installation assembly and the rotating assembly being connected by a connecting assembly, the installation assembly including a mounting base mounted on the top of the connecting assembly, a plurality of clamping mechanisms symmetrically disposed inside the mounting base, the clamping mechanisms being slidably mounted inside the mounting base via a positioning mechanism, a support mechanism disposed inside the water bath tank, the clamping mechanism including two movable blocks slidably mounted inside the mounting base, a rotating seat rotatably connected between the two movable blocks, two mounting shafts rotatably connected inside the rotating seat, a clamping seat fixedly connected between the two mounting shafts, the rotation axis of the rotating seat and the rotation axis of the clamping seat being coplanarly and perpendicularly intersecting.

[0006] Preferably, the clamping seat has symmetrical through slots on its outer side, and a clamping block is slidably installed inside each through slot. The top of the clamping seat has an annular groove, and a connecting ring is rotatably connected inside the annular groove. A spiral strip is fixedly connected to the bottom of the connecting ring. A fixed shaft is fixedly connected to the top of the clamping block, and the fixed shaft is slidably installed inside the spiral strip.

[0007] Preferably, a rotating block is fixedly connected to the top of the connecting ring, and a gasket is fixedly connected to one of the adjacent ends of the two clamping blocks.

[0008] Preferably, the positioning mechanism includes two movable frames slidably connected to the upper and lower sides of the mounting base, four connecting blocks are fixedly connected between the two movable frames, the upper and lower ends of the movable blocks are respectively fixedly connected to the two movable frames, and a positioning bolt is threadedly installed on the top movable frame.

[0009] Preferably, the support mechanism includes a support bar fixedly connected inside the water bath tank, a guide sleeve fixedly connected to the top of the support bar, the number of guide sleeves being equal to the number of clamping mechanisms, a connecting rod slidably connected inside the guide sleeve, a spherical rotating block fixedly connected to the top of the connecting rod, a connecting sleeve rotatably connected to the outer side of the spherical rotating block, a support base fixedly connected to the top of the connecting sleeve, and a spring fixedly connected between the bottom of the connecting rod and the support bar.

[0010] Preferably, the connecting assembly includes two synchronization frames and four connecting frames. The two synchronization frames are symmetrically slidably installed on the bottom of the device housing. Each connecting frame has a bolt penetrating its top, and the bottom of the bolt is threaded to the top of the synchronization frame. The bottom of the four connecting frames is fixedly connected to the mounting base.

[0011] Preferably, a motor is fixedly installed at the bottom of the water bath, a drive gear is fixedly installed on the output end of the motor, and support blocks are fixedly connected at the four corners of the bottom of the water bath.

[0012] Preferably, the rotating assembly includes a fixed base fixedly connected to the inner wall of the bottom of the device housing. Two auxiliary gears and an end face gear are rotatably connected to the top of the fixed base. The driving gear meshes with the top of the end face gear to drive the end face gear to rotate. A main gear is fixedly connected to the outer side of the end face gear. The two ends of the main gear mesh with the two auxiliary gears respectively. A connecting shaft is fixedly connected to the top of the auxiliary gear. The connecting shaft passes through the synchronization frame and is rotatably connected to it.

[0013] Preferably, the tops of the two connecting shafts are rotatably connected to the same synchronization bar, and the upper surface of the synchronization frame and the lower surface of the synchronization bar are in contact with each other.

[0014] Preferably, four limiting sleeves are fixedly connected to the top of the fixed base, and both ends of the synchronization frame are slidably connected between the limiting sleeves and the fixed base.

[0015] Compared with the prior art, the beneficial effects of the present invention are: the present invention has a reasonable structural design and strong functionality, and has the following advantages: 1. The rotating connection between the rotating seat and the movable block in the clamping mechanism, along with the rotating connection between the clamping seat and the rotating seat, and the coplanar and perpendicular intersection of their rotation axes, allows for adjustment of the tilt angle of the culture dish. This ensures uniform force on the rotating cells within the culture dish, improving culture quality. The support mechanism uses the elastic restoring force of the spring to keep the support seat in close contact with the bottom of the culture dish, achieving elastic support. The rotational cooperation between the spherical rotating block and the connecting sleeve allows the support seat to rotate synchronously with the angle adjustment of the culture dish, achieving dynamic adaptive support and avoiding damage to the culture dish caused by rigid support. At the same time, it effectively prevents the culture dish from tilting or flipping during rotation, ensuring culture safety.

[0016] 2. The rotating assembly adopts a meshing transmission structure of end face gears, main gears, and double auxiliary gears, with a synchronizing bar to limit the synchronization of the two connecting shafts. This effectively reduces the transmission error caused by gear meshing clearance and avoids jamming and shaking during rotation. The limiting sleeve limits the sliding of the synchronizing frame, further ensuring the smooth rotation of the synchronizing frame and the mounting assembly. This allows the cells in the culture dish to be evenly suspended, avoiding hypoxia and uneven nutrient distribution caused by local precipitation, and significantly improving the cell culture effect. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is a schematic diagram of the rotating component structure of the present invention; Figure 3 This is a schematic diagram of the water bath structure of the present invention; Figure 4 This is a schematic diagram of the bottom structure of the water bath tank of the present invention; Figure 5 This is a schematic diagram of the mounting component, connecting component, and rotating component of the present invention; Figure 6 This is a schematic diagram of the mounting base and connecting frame structure of the present invention; Figure 7 This is a schematic diagram of the positioning mechanism structure of the present invention; Figure 8 This is a schematic diagram of the support mechanism structure of the present invention; Figure 9 This is a schematic diagram of the clamping mechanism of the present invention; Figure 10 For the present invention Figure 9 Schematic diagram of the structure at point A in the middle; Figure 11 This is a schematic diagram of the cross-sectional structure of the limiting sleeve of the present invention; Figure 12 This is a schematic diagram of the fixing base structure of the present invention; Figure 13 This is a schematic cross-sectional view of the fixing seat of the present invention.

[0018] In the diagram: 1. Device housing; 2. Water bath tank; 3. Mounting assembly; 31. Mounting base; 32. Clamping mechanism; 321. Rotating seat; 322. Movable block; 323. Rotating block; 324. Clamping seat; 325. Mounting shaft; 326. Connecting ring; 327. Spiral strip; 328. Clamping block; 329. Fixed shaft; 320. Gasket; 3201. Through groove; 3202. Annular groove; 33. Positioning mechanism; 331. Movable frame; 332. Connecting block; 333. Positioning bolt; 34. Support mechanism; 341. Support bar; 342. Support seat; 343. Guide sleeve; 344. Connecting sleeve; 345. Spherical rotating block; 346. Connecting rod; 347. Spring; 4. Connecting assembly; 41. Synchronizing frame; 42. Connecting frame; 43. Bolt; 5. Rotating assembly; 51. Fixed seat; 52. Secondary gear; 53. Main gear; 54. Synchronizing bar; 55. Limiting sleeve; 56. End face gear; 57. Connecting shaft; 6. Support block; 7. Motor; 8. Drive gear. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] Please see Figures 1 to 13This invention provides a technical solution: a cell culture rotation isolation device, including a device housing 1, a water bath 2 detachably installed inside the device housing 1, an installation component 3 inside the water bath 2, and a rotating component 5 between the device housing 1 and the water bath 2 to facilitate the rotation and shaking of the installation component 3 within the water bath 2. The installation component 3 and the rotating component 5 are connected by a connecting component 4 to transmit the rotational and shaking force, and the connecting component 4 also facilitates the installation of the water bath 2 within the device housing 1. The installation component 3 includes a mounting base 31 mounted on the top of the connecting component 4. Multiple clamping mechanisms 32 are symmetrically arranged inside the mounting base 31. The clamping mechanisms 32 are slidably mounted inside the mounting base 31 via a positioning mechanism 33. The positioning mechanism 33 limits the movement of the mounting base 31, ensuring stability while allowing for... The clamping mechanism 32 is fixed in position after movement. The water bath 2 is equipped with a support mechanism 34 to support the bottom of the culture dish and ensure that the culture dish remains stable during rotation and shaking. The clamping mechanism 32 includes two movable blocks 322 that are slidably installed inside the mounting base 31. A rotating seat 321 is rotatably connected between the two movable blocks 322. A cylindrical groove is provided on the movable block 322. Two cylindrical protrusions are symmetrically provided on the outer side of the rotating seat 321. The rotating seat 321 is rotatably connected to the cylindrical groove on the movable block 322 through the cylindrical protrusions. Two mounting shafts 325 are rotatably connected inside the rotating seat 321. A clamping seat 324 is fixedly connected between the two mounting shafts 325. The rotation axis of the rotating seat 321 and the rotation axis of the clamping seat 324 are coplanar and perpendicularly intersecting, so that the two axes are in the same plane and the included angle is ninety degrees, which can realize multi-angle rotation.

[0021] The clamping base 324 has symmetrical through grooves 3201 on its outer side. A clamping block 328 is slidably installed inside each through groove 3201 to ensure that the clamping block 328 moves stably within the clamping base 324. The top of the clamping base 324 has an annular groove 3202. A connecting ring 326 is rotatably connected inside the annular groove 3202 to ensure that the connecting ring 326 rotates stably within the clamping base 324. A spiral strip 327 is fixedly connected to the bottom of the connecting ring 326. The spiral strip 327 is arranged horizontally, and the axial center lines of the connecting ring 326 and the spiral strip 327 are collinear. A fixed shaft 329 is fixedly connected to the top of the clamping block 328. The fixed shaft 329 is slidably installed inside the spiral strip 327. By sliding the fixed shaft 329 in the spiral groove inside the spiral strip 327, the spiral strip 327 can push the two symmetrically arranged fixed shafts 329 to move synchronously in opposite directions during rotation, thereby completing the clamping and releasing of the cell culture dish.

[0022] A rotating block 323 is fixedly connected to the top of the connecting ring 326. By rotating the rotating block 323, the connecting ring 326 fixedly connected to its bottom can be easily rotated. When the rotation is manually stopped, the connecting ring 326 will stop rotating through the rotation cooperation in the clamping seat 324. At this time, a stable support and limiting structure is formed between the fixed shaft 329 and the spiral strip 327, ensuring that the fixed shaft 329 drives the clamping block 328 to be fixed in position in the clamping seat 324. A gasket 320 is fixedly connected to one end of the two clamping blocks 328 that is close to each other. The clamping stability is improved by the clamping friction between the gasket 320 and the culture dish.

[0023] The positioning mechanism 33 includes two movable frames 331 slidably connected to the upper and lower sides of the mounting base 31. Four connecting blocks 332 are fixedly connected between the two movable frames 331. The connecting blocks 332 are synchronously inserted through and slidably installed inside the mounting base 31 with the clamping mechanism 32. This allows the clamping mechanism 32 to limit its movement position by contacting the inner wall of the mounting base 31 through the connecting blocks 332. The connecting blocks 332 also connect the two movable frames 331, ensuring that the two movable frames 331 can stably fit against the upper and lower surfaces of the mounting base 31 and move together. The upper and lower ends of the movable blocks 322 are fixedly connected to the two movable frames 331 respectively. A positioning bolt 333 is threaded onto the top movable frame 331. By rotating the positioning bolt 333, the bottom of the positioning bolt 333 abuts against the upper surface of the mounting base 31, locking the position of the movable frame 331. This ensures that after the position is adjusted, the petri dish can rotate and sway stably with the overall movement of the mounting base 31.

[0024] The support mechanism 34 includes a support bar 341 fixedly connected inside the water bath 2. A guide sleeve 343 is fixedly connected to the top of the support bar 341. The number of guide sleeves 343 is equal to the number of clamping mechanisms 32. The guide sleeves 343, in cooperation with the clamping mechanisms 32, support the culture dish, ensuring that the culture dish can stably rotate and sway with the mounting base 31. A connecting rod 346 is slidably connected inside the guide sleeve 343, ensuring the stability of the connecting rod 346's vertical movement. A spherical rotating block 345 is fixedly connected to the top of the connecting rod 346, and a connecting sleeve 344 is rotatably connected to the outside of the spherical rotating block 345. Through the rotatable connection between the connecting sleeve 344 and the spherical rotating block 345, the support base 342 can synchronously adjust its angle when the culture dish rotates with the clamping mechanism 32, and the culture dish rotates and sways with the mounting base 31. During operation, the connecting sleeve 344 rotates on top of the spherical rotating block 345 to ensure stable support and limit the bottom of the culture dish, enabling rotation while the culture dish is tilted. This reduces sedimentation during static settling, maintains cell suspension, and prevents hypoxia or nutrient deficiency. A support base 342 is fixedly connected to the top of the connecting sleeve 344, and a spring 347 is fixedly connected between the bottom of the connecting rod 346 and the support bar 341. During the installation of the culture dish, the bottom of the culture dish is inserted into the support base 342 and then pressed down, causing the support base 342 to compress the spring 347 through the connecting sleeve 344, the spherical rotating block 345, and the connecting rod 346. When the culture dish is fixedly clamped in the clamping mechanism 32 and the top moves, the spring 347 will push the support base 342 to always be in contact with the bottom of the culture dish under the elastic force. After the clamping mechanism 32 completes the position adjustment, the spring 347 returns to its normal state.

[0025] The connecting assembly 4 includes two synchronization frames 41 and four connecting frames 42. The two synchronization frames 41 are symmetrically slidably installed on the bottom of the device housing 1. The bottom of the synchronization frame 41 is slidably installed through the bottom of the inner cavity of the device housing 1, and its two ends extend to the outside of the device housing 1 so that the synchronization frame 41 can rotate circumferentially under the drive of the rotating assembly 5. The top of each connecting frame 42 is penetrated by a bolt 43, and the bottom of the bolt 43 is threaded to the top of the synchronization frame 41. The bottom of the four connecting frames 42 extends into the top opening of the water bath tank 2 and is fixedly connected to the mounting base 31. The synchronization frame 41 and the mounting base 31 can be disassembled by the bolt 43 installed on the top of the part of the synchronization frame 41 that extends through the device housing 1. The disassembled synchronization frame 41 facilitates the removal of the water bath tank 2 from the device housing 1.

[0026] A motor 7 is fixedly installed at the bottom of the water bath tank 2. A drive gear 8 is fixedly installed on the output end of the motor 7. Support blocks 6 are also fixedly connected at the four corners of the bottom of the water bath tank 2 to support the water bath tank 2 inside the device housing 1.

[0027] The rotating assembly 5 includes a fixed base 51 fixedly connected to the inner wall of the bottom of the device housing 1. Two auxiliary gears 52 and an end face gear 56 are rotatably connected to the top of the fixed base 51. The auxiliary gears 52 and the end face gear 56 are rotatably mounted by opening grooves on the upper surface of the fixed base 51 that are adapted to the auxiliary gears 52 and the end face gear 56. The drive gear 8 meshes with the top of the end face gear 56 to drive the end face gear 56 to rotate. A main gear 53 is fixedly connected to the outside of the end face gear 56. The two ends of the main gear 53 mesh with the two auxiliary gears 52 respectively. A connecting shaft 57 is fixedly connected to the top of the auxiliary gears 52. The connecting shaft 57 passes through the synchronization frame 41 and is rotatably connected to it. Through the rotatable connection between the connecting shaft 57 and the synchronization frame 41, the connecting shaft 57 can drive the synchronization frame 41 to rotate synchronously when it rotates in a circle with the auxiliary gears 52.

[0028] The top of the two connecting shafts 57 are rotatably connected to the same synchronizing bar 54. The upper surface of the synchronizing frame 41 and the lower surface of the synchronizing bar 54 are in contact with each other. The synchronizing bar 54 can realize the rotation synchronization between the two auxiliary gears 52, ensuring that the two synchronizing frames 41 can rotate synchronously and reducing the influence of meshing tolerance on the synchronous rotation of the two synchronizing frames 41.

[0029] Four limiting sleeves 55 are fixedly connected to the top of the fixed base 51. Both ends of the synchronization frame 41 are slidably connected between the limiting sleeves 55 and the fixed base 51. By sliding the bottom of the synchronization frame 41 between the synchronization frame 41 and the limiting sleeves 55, the synchronization frame 41 can stably rotate as a whole under the drive of the secondary gear 52.

[0030] In practical use, its working principle is as follows: Installation and positioning of the culture dish: First, place the cell culture dish to be cultured between the clamping mechanism 32 and the support mechanism 34 of the installation component 3. In specific operation, first embed the bottom of the culture dish into the support seat 342 of the support mechanism 34, press the culture dish down to cause the support seat 342 to drive the spherical rotating block 345 and the connecting rod 346 to slide along the axial direction of the guide sleeve 343 through the connecting sleeve 344, thereby compressing the spring 347 between the connecting rod 346 and the support bar 341; Then, the rotating block 323 at the top of the clamping mechanism 32 is rotated, which drives the connecting ring 326 fixedly connected to its bottom to rotate along the annular groove 3202 at the top of the clamping seat 324. During the rotation of the connecting ring 326, the spiral strip 327 at the bottom is rotated synchronously. Since the fixed shaft 329 at the top of the clamping block 328 is slidably embedded in the spiral groove of the spiral strip 327, the rotational motion of the spiral strip 327 is converted into the linear motion of the fixed shaft 329, which drives the two symmetrically arranged clamping blocks 328 to move synchronously towards each other along the through groove 3201 until the pad 320 on the inner side of the clamping block 328 is tightly attached to the outer wall of the culture dish, thus completing the clamping and fixing of the culture dish. During this process, the spring 347 is always in a compressed state, and its elastic restoring force is transmitted to the support 342 through the connecting rod 346, the spherical rotating block 345 and the connecting sleeve 344, so that the support 342 is always in close contact with the bottom of the culture dish, achieving initial support and limiting.

[0031] Position adjustment and locking of clamping mechanism 32: According to the specifications of the petri dish and the cultivation requirements, the position of clamping mechanism 32 in the mounting base 31 can be adjusted by positioning mechanism 33. Specifically, the movable frame 331 is pushed. Since the movable frame 331 is fixedly connected to the movable block 322 of clamping mechanism 32 through connecting block 332, and the connecting block 332 slides through the mounting base 31, the movement of movable frame 331 can synchronously drive clamping mechanism 32 to slide along the length direction of mounting base 31. After the clamping mechanism 32 is adjusted to the target position, rotate the positioning bolt 333 on the top movable frame 331 so that the bottom of the positioning bolt 333 is in close contact with the upper surface of the mounting base 31. The position of the movable frame 331 and the clamping mechanism 32 is locked by friction to prevent displacement during subsequent rotation.

[0032] Water bath 2 assembly: When it is necessary to remove the water bath 2, the connection between the connecting frame 42 and the synchronization frame 41 can be disassembled by unscrewing the bolt 43, and the water bath 2 can be slid out along the inside of the device shell 1 for convenient maintenance.

[0033] Rotational power transmission and swaying realization: Start the motor 7 at the bottom of the water bath tank 2. The output end of the motor 7 drives the drive gear 8 to rotate. Since the drive gear 8 meshes with the top of the end face gear 56 of the rotating component 5, the rotational power of the drive gear 8 is transmitted to the end face gear 56, which drives the end face gear 56 to rotate along the fixed seat 51 on the bottom inner wall of the device housing 1. The main gear 53 on the outer side of the end face gear 56 meshes with two auxiliary gears 52. The rotation of the end face gear 56 drives the main gear 53 and the two auxiliary gears 52 to rotate synchronously. The connecting shaft 57 at the top of the auxiliary gear 52 rotates synchronously with the auxiliary gear 52. The connecting shaft 57 passes through the synchronization frame 41 and is rotatably connected to it. The two ends of the synchronization frame 41 are slidably embedded between the limiting sleeve 55 on the top of the fixed seat 51 and the fixed seat 51. The rotation of the connecting shaft 57 pushes the synchronization frame 41 to make a circular motion along the trajectory defined by the limiting sleeve 55. The tops of the two connecting shafts 57 are rotatably connected by a synchronizing bar 54. The synchronizing bar 54 can ensure the rotational synchronization of the two auxiliary gears 52 and the connecting shafts 57, avoid the meshing tolerance from causing the two synchronizing frames 41 to move asynchronously, and thus ensure the smooth rotation of the mounting base 31 and the culture dish.

[0034] Multi-angle rotation and stable support in synergy: During the rotation and swaying of the petri dish with the mounting base 31, the clamping mechanism 32 can realize the multi-angle posture adjustment of the petri dish. Since the rotating base 321 is rotatably connected to the movable block 322, and the clamping base 324 is rotatably connected to the rotating base 321 through the mounting shaft 325, and the rotation axis of the rotating base 321 and the rotation axis of the clamping base 324 are coplanar and perpendicularly intersecting at an angle of 90 degrees, the rotation of the petri dish at the tilt angle can be realized by adjusting the rotation angle of the rotating base 321 and the clamping base 324. Meanwhile, the connecting sleeve 344 of the support mechanism 34 rotates in conjunction with the spherical rotating block 345. When the culture dish rotates and shakes, the support seat 342 can rotate flexibly around the spherical rotating block 345 through the connecting sleeve 344. With the elastic pre-tightening force of the spring 347, the support seat 342 is always in close contact with the bottom of the culture dish, achieving dynamic and stable support. The constant temperature environment in the water bath 2 can maintain the temperature required for cell culture. Combined with the rotation and shaking, the cells in the culture dish are always in a suspended state, avoiding local hypoxia and uneven nutrient distribution caused by cell precipitation, thus ensuring the cell culture effect.

[0035] In summary, after the culture dish is clamped, the positioning mechanism 33 moves the clamping mechanism 32 to adjust the tilt of the culture dish. During this process, the support mechanism 34 always supports the bottom of the culture dish, so that when the rotating component 5 rotates under the drive of the motor 7, the rotating component 5 drives the mounting base 31 and the positioning mechanism 33 and clamping mechanism 32 on the mounting base 31 to rotate synchronously through the connecting component 4. Since the bottom of the culture dish is inside the support mechanism 34, and the connecting sleeve 344 and the spherical rotating block 345 rotate and cooperate, the top of the culture dish moves with the mounting base 31 and rotates around the connection point of the connecting sleeve 344 and the spherical rotating block 345 as the center of rotation, thereby realizing the rotation and shaking of the culture dish.

[0036] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A cell culture rotating isolation device, comprising a device housing (1), wherein a water bath (2) is detachably installed inside the device housing (1), characterized in that: An installation assembly (3) is provided inside the water bath tank (2). A rotating assembly (5) is provided between the device housing (1) and the water bath tank (2). The installation assembly (3) and the rotating assembly (5) are connected by a connecting assembly (4). The installation assembly (3) includes a mounting base (31) mounted on the top of the connecting assembly (4). Multiple clamping mechanisms (32) are symmetrically arranged inside the mounting base (31). The clamping mechanisms (32) are slidably mounted inside the mounting base (31) by a positioning mechanism (33). The bath box (2) is provided with a support mechanism (34). The clamping mechanism (32) includes two movable blocks (322) that are slidably installed inside the mounting base (31). A rotating seat (321) is rotatably connected between the two movable blocks (322). Two mounting shafts (325) are rotatably connected inside the rotating seat (321). A clamping seat (324) is fixedly connected between the two mounting shafts (325). The rotation axis of the rotating seat (321) and the rotation axis of the clamping seat (324) are coplanar and perpendicularly intersecting.

2. The cell culture rotating isolation device according to claim 1, characterized in that: The clamping seat (324) has symmetrical through grooves (3201) on its outer side. A clamping block (328) is slidably installed inside each through groove (3201). The clamping seat (324) has an annular groove (3202) on its top. A connecting ring (326) is rotatably connected inside the annular groove (3202). A spiral strip (327) is fixedly connected to the bottom of the connecting ring (326). A fixed shaft (329) is fixedly connected to the top of the clamping block (328). The fixed shaft (329) is slidably installed inside the spiral strip (327).

3. The cell culture rotating isolation device according to claim 2, characterized in that: A rotating block (323) is fixedly connected to the top of the connecting ring (326), and a gasket (320) is fixedly connected to one of the two clamping blocks (328) at their adjacent ends.

4. The cell culture rotating isolation device according to claim 1, characterized in that: The positioning mechanism (33) includes two movable frames (331) slidably connected to the upper and lower sides of the mounting base (31). Four connecting blocks (332) are fixedly connected between the two movable frames (331). The upper and lower ends of the movable blocks (322) are fixedly connected to the two movable frames (331) respectively. A positioning bolt (333) is threaded on the movable frame (331) at the top.

5. The cell culture rotating isolation device according to claim 1, characterized in that: The support mechanism (34) includes a support bar (341) fixedly connected inside the water bath tank (2). A guide sleeve (343) is fixedly connected to the top of the support bar (341). The number of guide sleeves (343) is equal to the number of clamping mechanisms (32). A connecting rod (346) is slidably connected inside the guide sleeve (343). A spherical rotating block (345) is fixedly connected to the top of the connecting rod (346). A connecting sleeve (344) is rotatably connected to the outside of the spherical rotating block (345). A support seat (342) is fixedly connected to the top of the connecting sleeve (344). A spring (347) is fixedly connected between the bottom of the connecting rod (346) and the support bar (341).

6. The cell culture rotating isolation device according to claim 1, characterized in that: The connecting assembly (4) includes two synchronization frames (41) and four connecting frames (42). The two synchronization frames (41) are symmetrically slidably installed on the bottom of the device housing (1). The top of each connecting frame (42) is penetrated by a bolt (43). The bottom of the bolt (43) is threaded to the top of the synchronization frame (41). The bottom of the four connecting frames (42) is fixedly connected to the mounting base (31).

7. A cell culture rotating isolation device according to claim 6, characterized in that: A motor (7) is fixedly installed at the bottom of the water bath (2), and a drive gear (8) is fixedly installed on the output end of the motor (7). Support blocks (6) are also fixedly connected at the four corners of the bottom of the water bath (2).

8. A cell culture rotating isolation device according to claim 7, characterized in that: The rotating assembly (5) includes a fixed base (51) fixedly connected to the inner wall of the bottom of the device housing (1). The top of the fixed base (51) is rotatably connected to two auxiliary gears (52) and an end face gear (56). The driving gear (8) meshes with the top of the end face gear (56) to drive the end face gear (56) to rotate. The outer side of the end face gear (56) is fixedly connected to a main gear (53). The two ends of the main gear (53) mesh with the two auxiliary gears (52) respectively. The top of the auxiliary gears (52) is fixedly connected to a connecting shaft (57). The connecting shaft (57) passes through the synchronization frame (41) and is rotatably connected to it.

9. A cell culture rotating isolation device according to claim 8, characterized in that: The tops of the two connecting shafts (57) are rotatably connected to the same synchronization bar (54), and the upper surface of the synchronization frame (41) and the lower surface of the synchronization bar (54) are in contact with each other.

10. A cell culture rotating isolation device according to claim 8, characterized in that: The top of the fixed base (51) is fixedly connected with four limiting sleeves (55), and both ends of the synchronization frame (41) are slidably connected between the limiting sleeves (55) and the fixed base (51).

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