Cell cloning loop culture dish

Abstract

The utility model discloses a cell cloning loop culture dish, which relates to the technical field of cell culture and comprises cell culture plates with uniform specifications. The cell culture plate is characterized by comprising a bottom plate which is arranged on the bottom surface of the cell culture plate; a cell cloning ring is arranged in the cell culture plate; a top cover is arranged at the upper end of the cell culture plate; the bottom plate and the cell cloning ring are fixed through magnetic attraction and have color difference; the cell cloning ring is arranged in the culture groove of the cell culture plate; the cell cloning ring fixing device has the beneficial effects that the original cell cloning ring is more accurately and stably fixed on the cell culture plate, and meanwhile, pancreatin is not leaked to a non-target area to digest cells. It is guaranteed that all cells obtained after digestion and elution are from the same cell clone cluster, and the purpose of accurately selecting monoclonal cell colonies is achieved.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a cell cloning ring culture dish. Background Technology

[0002] Existing cell cloning loops for selecting single-cell colonies are made of ordinary glass rings. They are placed on pre-selected single-cell colonies grown on a cell culture plate to limit the area of ​​cell digestion and elution during extraction. However, this tool is too simple in structure and cannot effectively fix itself to the cell culture plate and seal the addition of trypsin to digest extracellular conjugates. This can lead to loop displacement, trypsin extravasation, and the collection of cells from non-target colonies during the procedure, resulting in unpredictable problems in subsequent experimental operations and cell culture. Utility Model Content

[0003] In this section, as well as in the abstract and title of this application, some simplifications or omissions may be made to avoid obscuring the purpose of this section, the abstract, and the title of this application. Such simplifications or omissions shall not be used to limit the scope of this utility model.

[0004] In view of the problems existing in the above or prior art, the present invention is proposed.

[0005] Therefore, the purpose of this invention is to provide a cell cloning loop for a culture dish, which solves the technical problems of unstable positioning, easy leakage, and unclear display of cell colonies in existing cell cloning loops.

[0006] To solve the aforementioned technical problem, this utility model provides the following technical solution: a cell cloning loop culture dish, which includes a cell culture plate and adopts a cell culture plate of uniform specifications;

[0007] A base plate is placed on the bottom surface of the cell culture plate; a cell cloning ring is provided inside the cell culture plate; a top cover is provided at the upper end of the cell culture plate; the base plate and the cell cloning ring are fixed together by magnetic attraction, and there is a color difference between the two; the cell cloning ring is placed in the culture tank of the cell culture plate.

[0008] As a preferred embodiment of the cell cloning ring culture dish of this utility model, the base plate is provided with a plurality of embedded placement stages, and the edge of the base plate is limited to the cell culture plate by a snap fastener.

[0009] As a preferred embodiment of the cell cloning loop culture dish of the present invention, wherein the cell cloning loop is provided with a glass ring, a magnetic ring and a silicone ring from top to bottom;

[0010] The glass ring is made of a colorless and transparent material;

[0011] The magnetic ring is made of magnetic material;

[0012] The silicone ring is made of a relatively soft material.

[0013] In a preferred embodiment of the cell cloning ring culture dish of this invention, the bottom plate and the upper surface of the placement stage are both coated with color.

[0014] The beneficial effects of this invention are as follows: This invention provides a cell cloning ring kit, which can not only better fix the cell cloning ring in the target position, but also better seal the trypsin that digests and elutes cells, while clearly displaying the selected cell colonies; this device can better help researchers accurately and efficiently select and extract monoclonal cell colonies. Attached Figure Description

[0015] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Wherein:

[0016] Figure 1 A schematic diagram of a cell cloning loop culture dish;

[0017] Figure 2 A schematic diagram showing the disassembly of a cell clone loop culture dish;

[0018] Figure 3 A schematic diagram of a cell cloning loop fitted onto a culture dish;

[0019] Figure 4 A schematic diagram of the parameters of a cell culture plate with a cell clone loop culture dish.

[0020] Marked in the image:

[0021] 100. Cell culture plate; 101. Culture tank; 200. Base plate; 201. Placement stage; 202. Snap fastener; 300. Cell cloning loop; 301. Glass loop; 302. Magnetic loop; 303. Silicone loop; 400. Top cover. Detailed Implementation

[0022] To make the objectives, features and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings.

[0023] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0024] Secondly, the term "an embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single embodiment or an embodiment selectively excluded from other embodiments. Example

[0025] Reference Figures 1-4 This is the first embodiment of the present invention. This embodiment provides a cell cloning ring culture dish, which includes a cell culture plate 100 and adopts a cell culture plate of uniform specifications.

[0026] Better, such as Figure 4 As shown, the 6-well culture plate conforms to the standard geometry and numbering (AB, 1-3) and meets all the important specifications of the ANSI / SLAS (SBS) standards (1-2004, 2-2004, 3-2004 and 4-2004).

[0027] A base plate 200 is placed on the bottom surface of a cell culture plate 100; a cell cloning ring 300 is provided inside the cell culture plate 100; a top cover 400 is provided at the upper end of the cell culture plate 100; the base plate 200 and the cell cloning ring 300 are fixed together by magnetic attraction, and there is a color difference between the two; the cell cloning ring 300 is placed in the culture tank 101 of the cell culture plate 100.

[0028] The base plate 200 is provided with several embedded placement stages 201, and the edge of the base plate 200 is limited to the cell culture plate 100 by the buckle 202.

[0029] Preferably, the placement stage 201 is equipped with an LED light that emits 350nm ultraviolet light. When used with the live cell dye Hoechst 33342, the location of monoclonal cell colonies can be clearly displayed by the fluorescence of the dye excited by ultraviolet light. When the light is off, the placement stage 201 has a white background, and when the light is on, it emits ultraviolet light.

[0030] The cell cloning loop 300 consists of a glass ring 301, a magnetic ring 302, and a silicone ring 303 from top to bottom;

[0031] The 301 glass ring is made of colorless and transparent material;

[0032] Preferably, the glass ring 301 is made of annular high borosilicate glass, which can withstand high temperatures.

[0033] Magnetic ring 302 is made of magnetic material;

[0034] The silicone ring 303 is made of a relatively soft material; preferably, the silicone ring 303 is made of silicone.

[0035] Preferably, the height of the glass ring 301 is 6mm, and the heights of the magnetic ring 302 and the silicone ring 303 are both 1mm.

[0036] Preferably, epoxy resin adhesive is used to bond the glass ring 301 and the magnetic ring 302, as well as the magnetic ring 302 and the silicone ring 303.

[0037] Preferably, the base plate 200 and the placement platform 201 are white; the base plate 200 uses a rectangular neodymium iron boron magnet as the base plate.

[0038] In use, first select and mark the target monoclonal cluster. Then, embed the magnetic base plate 200 under the cell culture plate 100. Use tweezers to align the center of the sterilized cell cloning loop 300 with the cell cloning colony, place it vertically on it, and attract it to adhere firmly. Then, add trypsin to the center of the cell cloning loop 300 to digest the cells, and aspirate the digested cell suspension to obtain a pure monoclonal colony.

[0039] In summary, a ring-shaped neodymium iron boron magnetic material and a ring-shaped silicone sealing ring are sequentially added to the bottom of the glass cloning loop. Additionally, a permanent magnet is fixed to the bottom of the cell culture plate; this white magnetic base increases contrast and better displays the location of single-cell clone markers. When selecting single-cell colonies, the colony locations are pre-marked. The magnetic base is then fixed to the bottom of the cell culture plate. The cloning loop is then placed with its center aligned with the single-cell colony. The magnetic attraction secures the loop to the target area, and the silicone sealing gasket provides anti-slip and sealing properties, preventing the loop from shifting and preventing trypsin from leaking into non-target areas to digest cells. This ensures that the cells obtained after digestion and elution all originate from the same cell clone cluster, achieving accurate selection of single-cell colonies.

[0040] It should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solution of this utility model without departing from the spirit and scope of the technical solution of this utility model, and all such modifications or substitutions should be covered within the scope of the claims of this utility model.

Claims

1. A cell cloning loop culture dish, comprising a cell culture plate (100), using a standardized cell culture plate; characterized in that: The system includes a base plate (200) placed on the bottom surface of the cell culture plate (100); a cell cloning ring (300) is provided inside the cell culture plate (100); a top cover (400) is provided at the upper end of the cell culture plate (100); the base plate (200) and the cell cloning ring (300) are fixed together by magnetic attraction, and there is a color difference between the two; the cell cloning ring (300) is placed in the culture tank (101) of the cell culture plate (100).

2. The cell cloning loop culture dish as described in claim 1, characterized in that: The base plate (200) is provided with several embedded placement platforms (201), and the edge of the base plate (200) limits the cell culture plate (100) by means of buckles (202).

3. The cell cloning loop culture dish as described in claim 1, characterized in that: The cell cloning ring (300) is provided with a glass ring (301), a magnetic ring (302) and a silicone ring (303) from top to bottom. The glass ring (301) is made of a colorless and transparent material; The magnetic ring (302) is made of magnetic material; The silicone ring (303) is made of a relatively soft material.

4. The cell cloning loop culture dish as described in claim 2, characterized in that: The placement stage (201) is equipped with an LED light that emits 350nm ultraviolet light.

Images