Constant temperature and humidity microbial agent culture equipment

Abstract

The present disclosure provides a constant temperature and humidity microbial agent culture device, comprising: a culture box, a box cover is slidingly fitted on the culture box, a rotating shaft is rotatably fitted in the culture box, a stirring structure is arranged on the circumferential side of the rotating shaft, the stirring structure comprises a plurality of stirring rods, a placing structure is arranged in the culture box, the placing structure comprises a plurality of placing plates, and the placing plates are fixedly connected with the stirring rods; a culture dish is fixedly connected on the placing plate, and a connecting structure is arranged between the stirring rod and the culture dish, the connecting structure comprises a communication pipe. In the constant temperature and humidity microbial agent culture device, not only a large amount of culture can be carried out through the culture box, but also culture can be carried out through the culture dish, so that the device is more flexible to use, and the culture medium and microorganisms can be stirred through the stirring rod, so that the nutrition liquid can be mixed with the microorganisms more uniformly when the nutrition liquid is added.

Description

Technical Field

[0001] This disclosure relates to the field of microbial inoculant cultivation technology, and in particular to a constant temperature and humidity microbial inoculant cultivation device. Background Technology

[0002] Microorganisms play a significant role in daily life, such as in water remediation and fertilizer fermentation. Most existing microbial cultivation methods involve petri dishes or incubators. To ensure microbial survival, a constant temperature and humidity environment is typically required. However, when sampling and feeding microorganisms in these petri dishes or incubators, the microorganisms are often directly exposed to external air, leading to the invasion of external microorganisms into their living environment. Furthermore, the sealed cultivation environment may result in insufficient oxygen supply or inadequate contact between the nutrient solution and the microorganisms, thus affecting the cultivation effect of the microbial agent and the survival rate of the microorganisms. Summary of the Invention

[0003] This disclosure aims to at least partially address one of the technical problems in the related art.

[0004] Therefore, the purpose of this disclosure is to provide a constant temperature and humidity microbial inoculant culture device.

[0005] To achieve the above objectives, this disclosure provides a constant temperature and humidity microbial inoculant cultivation device, comprising: an incubator with a slidingly fitted lid, a rotating shaft rotatably fitted inside the incubator, a stirring structure including multiple stirring rods mounted on the periphery of the rotating shaft, a placement structure including multiple placement plates inside the incubator, the placement plates being snapped and fixed to the stirring rods; a culture dish snapped and fixed to the placement plates, a connecting structure including a connecting pipe between the stirring rods and the culture dish, the connecting pipe communicating with the stirring rods and the culture dish, a plug elastically fitted inside the stirring rod, the stirring rod communicating with the rotating shaft; a liquid delivery structure including a nutrient solution tank and a spray head, the spray head communicating with the rotating shaft, a heating structure including an air heater mounted at the bottom of the incubator, a discharge pipe communicating with the rotating shaft at the bottom of the incubator, a sampling structure including a plug, multiple sampling ports opened on the periphery of the plug.

[0006] Optionally, an electric slide rail is fixed to the side of the incubator, and a sliding frame is fixed to the output end of the electric slide rail. The sliding frame is fixedly connected to the cover, and a motor is fixed to the cover. The output end of the motor is fixedly connected to the rotating shaft. The top of the incubator is provided with a sealing groove, and a sealing block is fixed to the lower side of the cover. The sealing block is located in the sealing groove.

[0007] Optionally, the liquid delivery structure further includes: a liquid pump, the nutrient solution tank is fixed to the tank cover, the liquid pump is fixed to the upper side of the nutrient solution tank, the spray head is fixed to the lower side of the tank cover, the liquid pump has an inlet pipe fixed to its inlet end and an outlet pipe fixed to its outlet end, the inlet pipe is connected to the nutrient solution tank, and the outlet pipe is connected to the spray head; wherein, the spray head has an annular structure, a connecting cavity is opened on the spray head, the connecting cavity is connected to the spray head, an electric valve is installed between the connecting cavity and the spray head, and the connecting cavity is connected to a rotating shaft.

[0008] Optionally, the rotating shaft has a cavity inside, and multiple first connecting ports are formed on the side of the cavity, which are connected to the cavity. Multiple second connecting ports are formed on the periphery of the cavity, which are connected to the incubator. A pressure valve is installed in the second connecting port. The pressure valve includes a plug plate. A rotating shaft is rotatably fitted inside the second connecting port. A torsion spring is fixed between the rotating shaft and the second connecting port. The rotating shaft is fixedly connected to the plug plate.

[0009] Optionally, a bottom plate is fixed inside the incubator, and the bottom plate is fixedly connected to the discharge pipe. The discharge pipe has a plug located inside it, and a groove is provided inside the plug. The groove is connected to the sampling port and the discharge pipe. The groove has a groove structure that is large at both ends and small in the middle. A piston is slidably fitted inside the groove, and multiple first springs are fixed between the piston and the groove wall. The groove is connected to the cavity.

[0010] Optionally, the heating structure further includes: a heat preservation chamber, which is located around the incubator. An air pump is fixed on one side of the incubator. The air outlet of the air pump is connected to the air inlet of an air heater. The air outlet of the air heater is connected to the heat preservation chamber. A three-way valve is installed at the bottom of the discharge pipe. The heat preservation chamber is connected to the three-way valve. An air outlet is opened on one side of the heat preservation chamber. An air inlet pipe is installed on the top of the nutrient solution tank.

[0011] Optionally, multiple connectors are fixed on the rotating shaft, and the connectors are fixedly connected to the stirring rod. A flow guide cavity is opened inside the stirring rod, and the flow guide cavity is connected to the rotating shaft. A block is slidably fitted inside the flow guide cavity, and multiple second springs are fixed between the block and the cavity wall of the flow guide cavity. The upper side of the stirring rod has multiple third connecting ports, and the lower side of the stirring rod has multiple fourth connecting ports. Both the third and fourth connecting ports are connected to the flow guide cavity. A slot is opened on the lower side of the placement plate, and the stirring rod is snapped and fixed in the slot.

[0012] Optionally, a pressing block is fixed to the lower side of the placement plate, and a through groove is opened on the stirring rod. The pressing block passes through the through groove and contacts the blocking block. A snap-fit ​​bracket is fixed to the placement plate, and the culture dish is snap-fitted and fixed in the snap-fit ​​bracket. A sealing cap is installed on the culture dish.

[0013] Optionally, the connecting tube is fixedly connected to the sealing cap, the connecting tube is connected to the petri dish, and one end of the connecting tube is located inside the stirring rod.

[0014] Optionally, a heat exchange chamber is provided inside the placement plate, and multiple fifth connecting ports are provided on the lower side of the heat exchange chamber. The fifth connecting ports are connected to the stirring rod, and multiple air outlets are provided on the side of the heat exchange chamber. An air pipe is fixed on one side of the incubator.

[0015] The technical solution provided in this disclosure may include the following beneficial effects: 1. A rotating shaft is installed inside the incubator, and a stirring rod is installed around the circumference of the rotating shaft. The stirring rod can be used to stir the microorganisms and liquid culture medium directly cultured in the incubator. The stirring rod can also be used to install the placement plate, so that microorganisms can be cultured in petri dishes. This allows the device to not only carry out large-scale culture in the incubator, but also to carry out culture in petri dishes, making the device more flexible in use. The stirring rod can also be used to stir the culture medium and microorganisms, so that when adding nutrient solution, it can be mixed more evenly with microorganisms, thereby ensuring the cultivation effect of microorganisms.

[0016] 2. The rotating shaft can assist in air intake and exhaust, and can also assist in adding nutrients into the culture dish. This ensures that microorganisms can have sufficient contact with oxygen and nutrient solution. It can also be used for exhaust to prevent excessive gas pressure caused by the microorganisms producing a large amount of gas during their survival. Furthermore, temperature and humidity can be controlled by introducing gases of different temperatures and humidity levels, thus ensuring a constant temperature and humidity state of the device and guaranteeing the survival rate of microorganisms.

[0017] 3. A sampling structure is installed at the bottom of the rotating shaft. Sampling and auxiliary venting can be carried out through the sampling structure, which can ensure that the internal environment of the device can be sampled without contact with the outside. This can prevent external bacteria from occupying the living space of internal microorganisms, ensure the survival rate of microorganisms, and facilitate the monitoring of the survival status of microorganisms, thus ensuring the cultivation effect of microorganisms.

[0018] Additional aspects and advantages of this disclosure will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of this disclosure. Attached Figure Description

[0019] The above and / or additional aspects and advantages of this disclosure will become apparent and readily understood from the following description of the embodiments taken in conjunction with the accompanying drawings, in which: Figure 1 This is a schematic diagram of the overall assembly three-dimensional structure of a constant temperature and humidity microbial agent cultivation device according to an embodiment of this disclosure; Figure 2This is a schematic diagram of the overall assembly cross-sectional structure of a constant temperature and humidity microbial agent cultivation device according to an embodiment of this disclosure; Figure 3 yes Figure 2 A schematic diagram at point A in the middle; Figure 4 yes Figure 2 A schematic diagram at point B in the middle; Figure 5 yes Figure 2 A schematic diagram at point C in the middle; Figure 6 This is a schematic diagram of the three-dimensional structure of the placement plate and culture dish in a constant temperature and humidity microbial agent culture device according to an embodiment of the present disclosure. Figure 7 This is an exploded view of the placement plate and petri dish in a constant temperature and humidity microbial agent cultivation device according to an embodiment of this disclosure; Figure 8 This is a schematic diagram of the assembly structure of the placement plate and stirring rod in a constant temperature and humidity microbial agent cultivation device according to an embodiment of this disclosure; Figure 9 This is a schematic cross-sectional view of the assembly structure of the placement plate and culture dish in a constant temperature and humidity microbial agent culture device according to an embodiment of this disclosure; Figure 10 This is a schematic cross-sectional view of the incubator and stirring rod in a constant temperature and humidity microbial agent cultivation device according to an embodiment of this disclosure; Figure 11 This is a schematic diagram of the assembly structure of the trachea and incubator in a constant temperature and humidity microbial agent cultivation device according to an embodiment of this disclosure; Figure 12 This is a schematic diagram of the assembly structure of the incubator and spray head in a constant temperature and humidity microbial agent cultivation device according to an embodiment of this disclosure; Figure 13 This is a schematic diagram of the assembly structure of the incubator and the bottom plate in a constant temperature and humidity microbial agent cultivation device according to an embodiment of this disclosure; As shown in the figure: 101, incubator; 102, electric slide rail; 103, sliding frame; 104, lid; 105, motor; 106, nutrient solution tank; 107, liquid pump; 108, inlet pipe; 109, outlet pipe; 110, spray head; 111, connecting cavity; 112, electric valve; 113, sealing groove; 114, sealing block; 201. Rotating shaft; 202. First connecting port; 203. Second connecting port; 204. Air pressure valve; 205. Blocking plate; 206. Rotating shaft; 207. Cavity; 208. Discharge pipe; 209. Plug; 210. Groove; 211. Piston; 212. Sampling port; 213. First spring; 214. Base plate; 301. Air heater; 302. Insulation cavity; 401. Connector; 402. Stirring rod; 403. Flow guide cavity; 404. Second spring; 405. Block; 406. Third connecting port; 407. Fourth connecting port; 408. Lowering block; 409. Through groove; 501. Placement plate; 502. Snap-fit ​​bracket; 503. Snap-fit ​​slot; 504. Fifth connecting port; 505. Heat exchange chamber; 506. Air outlet; 507. Air pipe; 601. Petri dish; 602. Sealing cap; 603. Connecting pipe. Detailed Implementation

[0020] Embodiments of this disclosure are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are used only to explain this disclosure, and should not be construed as limiting this disclosure. Rather, embodiments of this disclosure include all variations, modifications, and equivalents falling within the spirit and scope of the appended claims.

[0021] like Figures 1 to 13 As shown in the embodiment of this disclosure, a constant temperature and humidity microbial agent cultivation device is proposed, comprising: an incubator 101, a cover 104 slidably fitted onto the incubator 101, a rotating shaft 201 rotatably fitted inside the incubator 101, a stirring structure mounted on the periphery of the rotating shaft 201, the stirring structure including multiple stirring rods 402, a placement structure inside the incubator 101 including multiple placement plates 501, the placement plates 501 being snapped and fixed to the stirring rods 402; and a culture dish 601, the culture dish 601 being snapped and fixed to the placement plate 501, a connecting structure being installed between the stirring rods 402 and the culture dish 601, the connecting structure including a connecting pipe 603. The connecting pipe 603 is connected to the stirring rod 402 and the culture dish 601. The stirring rod 402 has a plug 405 that fits elastically inside. The stirring rod 402 is connected to the rotating shaft 201. The liquid delivery structure includes a nutrient solution tank 106 and a spray head 110. The spray head 110 is connected to the rotating shaft 201. The bottom of the culture box 101 is equipped with a heating structure, which includes an air heater 301. The bottom of the culture box 101 is equipped with a discharge pipe 208, which is connected to the rotating shaft 201. The bottom of the rotating shaft 201 is equipped with a sampling structure, which includes a plug 209. Multiple sampling ports 212 are opened on the periphery of the plug 209.

[0022] Specifically, microbial agents can be directly cultured in incubator 101. Biosensors, biochemical sensors, cell sensors, temperature sensors, and humidity sensors are installed inside incubator 101. A controller is installed on one side of incubator 101, allowing for real-time monitoring of the microbial survival status within the incubator. Sampling can also be performed using a sampling structure, followed by analysis using microbial detection and analysis instruments. This ensures the quality of microbial culture. Microorganisms can be cultured in petri dishes 601. By setting multiple placement plates 501, the number of petri dishes 601 can be increased, thereby increasing the bacterial culture volume. During culture in incubator 101, nutrient solution can be added through spray head 110, and stirring can be performed using stirring rod 402 to ensure a more uniform mixture of microbial agents and culture medium, thus improving the survival rate of microorganisms.

[0023] In this embodiment, an electric slide rail 102 is fixed to the side of the incubator 101, and a sliding frame 103 is fixed to the output end of the electric slide rail 102. The sliding frame 103 is fixedly connected to the cover 104, and a motor 105 is fixed on the cover 104. The output end of the motor 105 is fixedly connected to the rotating shaft 201. A sealing groove 113 is provided on the top of the incubator 101, and a sealing block 114 is fixed to the lower side of the cover 104. The sealing block 114 is located in the sealing groove 113.

[0024] Specifically, the electric slide rail 102 can drive the lid 104 to slide up and down, firstly adjusting the position of the rotating shaft 201 and the stirring rod 402 within the incubator 101, thus ensuring more uniform stirring. Furthermore, when using a petri dish 601 for microbial culture, the height of the petri dish 601 can be adjusted by moving the rotating shaft 201 up and down, facilitating the installation and removal of the petri dish 601. During culture, the sealing block 114 is located within the sealing groove 113, ensuring the device's airtightness and preventing contact with the external environment during culture, which could lead to external bacteria encroaching on the microorganisms' living space, thereby ensuring the survival rate of the microorganisms. The relatively long structure of the sealing block 114 also ensures the airtightness of the rotating shaft 201 during its up-and-down movement within a certain range, further improving the microbial culture effect and increasing the success rate of the culture.

[0025] The liquid delivery structure also includes: a liquid pump 107, the nutrient solution tank 106 is fixed on the tank cover 104, the liquid pump 107 is fixed on the upper side of the nutrient solution tank 106, the spray head 110 is fixed on the lower side of the tank cover 104, the inlet end of the liquid pump 107 is fixed with an inlet pipe 108, the outlet end of the liquid pump 107 is fixed with an outlet pipe 109, the inlet pipe 108 is connected to the nutrient solution tank 106, and the outlet pipe 109 is connected to the spray head 110; wherein, the spray head 110 has an annular structure, a connecting cavity 111 is opened on the spray head 110, the connecting cavity 111 is connected to the spray head 110, an electric valve 112 is installed between the connecting cavity 111 and the spray head 110, and the connecting cavity 111 is connected to the rotating shaft 201.

[0026] Specifically, when nutrient solution needs to be added, the liquid pump 107 is started, which draws out the nutrient solution from the nutrient solution tank 106 and sends it into the spray head 110. The nutrient solution is then sprayed out through the spray head 110, allowing it to enter the incubator 101 for the cultivation of microorganisms. When the stirring rod 402 is used for auxiliary addition, the electric valve 112 is started, connecting the connecting chamber 111 to the spray head 110. The nutrient solution inside the spray head 110 can then enter the rotating shaft 201 through the connecting chamber 111, and then enter the stirring rod 402, and be sprayed out directly. At this time, the nutrient solution can be directly delivered into the culture medium or petri dish 601, thus ensuring that the nutrient solution is added more evenly and ensuring the quality of microbial cultivation.

[0027] A cavity 207 is formed inside the rotating shaft 201. Multiple first connecting ports 202 are formed on the side of the cavity 207, which are connected to the connecting cavity 111. Multiple second connecting ports 203 are formed on the periphery of the cavity 207, which are connected to the incubator 101. A pressure valve 204 is installed in the second connecting port 203. The pressure valve 204 includes a blocking plate 205. A rotating shaft 206 is rotatably fitted inside the second connecting port 203. A torsion spring is fixed between the rotating shaft 206 and the second connecting port 203. The rotating shaft 206 is fixedly connected to the blocking plate 205.

[0028] Specifically, the nutrient solution can enter the first connecting port 202 from the connecting cavity 111, and then enter the cavity 207, thus enabling the rotating shaft 201 to assist in adding the nutrient solution. When the air pressure inside the incubator 101 is high, this high air pressure will relatively push up the blocking plate 205, causing the blocking plate 205 to rotate via the rotating shaft 206, opening the second connecting port 203. This allows the second connecting port 203 to connect with the incubator 101, and the gas inside the incubator 101 can be blown out through the rotating shaft 201, thereby achieving decompression operation. This prevents excessive air pressure caused by microbial activity from affecting the safety of the device and prevents excessive air pressure from affecting the device's performance. The survival of microorganisms is affected; when relatively dry or relatively humid gas is introduced through the rotating shaft 201, air can be introduced into the rotating shaft 201, making the air pressure in the cavity 207 higher, thereby relatively squeezing the blocking plate 205 outward, thus realizing the introduction of humid or dry air into the incubator 101. By cooperating with the humidity sensor, the incubator 101 can achieve a better supply and humidity control effect, ensuring that the incubator 101 is always in a constant humidity state. Furthermore, by introducing air at different temperatures, a better temperature control effect can also be achieved, thereby ensuring that the device is in a constant temperature state, thus improving the cultivation effect of microbial agents and ensuring the survival rate of microbial agents.

[0029] A base plate 214 is fixed inside the incubator 101. The base plate 214 is fixedly connected to the discharge pipe 208. The plug 209 is located inside the discharge pipe 208. A groove 210 is formed inside the plug 209. The groove 210 is connected to the sampling port 212 and the discharge pipe 208. The groove 210 has a groove structure that is large at both ends and small in the middle. A piston 211 is slidably fitted inside the groove 210. Multiple first springs 213 are fixed between the piston 211 and the groove wall of the groove 210. The groove 210 is connected to the cavity 207.

[0030] Specifically, when microorganisms are cultured directly in incubator 101, the microorganisms are cultured through the space above the bottom plate 214. After cultivation, for liquid culture medium, the lid 104 can be moved upward a certain distance, causing the plug 209 to slide upward away from the discharge pipe 208, allowing the liquid culture medium to flow out from the discharge pipe 208. This achieves the collection of microorganisms after cultivation, thereby improving work efficiency, reducing the residue of microorganisms and culture medium in incubator 101, reducing waste, and facilitating subsequent sterilization and disinfection of incubator 101. This facilitates the success rate of subsequent microbial cultivation. During the cultivation process, when sampling is required, the lid 104 can be moved upwards a certain distance, allowing the plug 209 to slide upwards until the sampling port 212 connects with the incubator 101. At this time, by manually pushing the piston 211 upwards through the guide rod, a portion of the liquid culture medium can flow directly from the sampling port 212 and the discharge pipe 208, thus achieving the sampling effect. This allows for the detection of microbial cultivation, facilitating timely adjustments, ensuring the cultivation effect of microorganisms, and improving their survival rate.

[0031] The heating structure also includes: a heat preservation cavity 302, which is located around the incubator 101. An air pump is fixed on one side of the incubator 101. The air outlet of the air pump is connected to the air inlet of the air heater 301. The air outlet of the air heater 301 is connected to the heat preservation cavity 302. A three-way valve is installed at the bottom of the discharge pipe 208. The heat preservation cavity 302 is connected to the three-way valve. An air outlet is opened on one side of the heat preservation cavity 302. An air inlet pipe is installed on the top of the nutrient solution tank 106.

[0032] Specifically, sterile air is pumped into the air heater 301 and heated to a suitable temperature as needed. The air is then blown into the insulation chamber 302, thus achieving better temperature control. Alternatively, the air in the insulation chamber 302 can be directly pumped into the rotating shaft 201 through a three-way valve, and then blown directly into the incubator 101 from the second connecting port 203, or directly blown out from the third connecting port 406 and the fourth connecting port 407 on the stirring rod 402. This ensures temperature uniformity and prevents the temperature from being high on the sides and low in the middle, thereby ensuring the survival rate of microorganisms.

[0033] Multiple connectors 401 are fixed on the rotating shaft 201, and the connectors 401 are fixedly connected to the stirring rod 402. A flow guide cavity 403 is formed inside the stirring rod 402, and the flow guide cavity 403 is connected to the rotating shaft 201. A block 405 is slidably fitted inside the flow guide cavity 403, and multiple second springs 404 are fixed between the block 405 and the cavity wall of the flow guide cavity 403. Multiple third connecting ports 406 are formed on the upper side of the stirring rod 402, and multiple fourth connecting ports 407 are formed on the lower side of the stirring rod 402. Both the third connecting ports 406 and the fourth connecting ports 407 are connected to the flow guide cavity 403. A slot 503 is formed on the lower side of the placement plate 501, and the stirring rod 402 is snapped and fixed inside the slot 503. A pressing block 408 is fixed on the lower side of the placement plate 501. A through groove 409 is provided on the upper part, and a lower pressing block 408 passes through the through groove 409 and contacts the blocking block 405. A snap-fit ​​bracket 502 is fixed on the placement plate 501, and a culture dish 601 is snap-fitted and fixed inside the snap-fit ​​bracket 502. A sealing cap 602 is installed on the culture dish 601, and a connecting pipe 603 is fixedly connected to the sealing cap 602 and communicates with the culture dish 601. One end of the connecting pipe 603 is located inside the stirring rod 402. A heat exchange chamber 505 is provided inside the placement plate 501, and multiple fifth connecting ports 504 are provided on the lower side of the heat exchange chamber 505. The fifth connecting ports 504 are communicated with the stirring rod 402. Multiple air outlets 506 are provided on the side of the heat exchange chamber 505, and multiple electric valves are installed inside the air outlets 506. An air pipe 507 is fixed on one side of the incubator 101.

[0034] Specifically, when culture dish 601 is needed for cultivation, it is placed in the clip holder 502 and secured by the clip holder 502. After inoculation with bacteria, the sealing cap 602 is placed on top, and then the placement plate 501 is clipped onto the stirring rod 402. At this time, under the action of the pressing block 408, the pressing block 408 is pressed downward, causing the blocking block 405 to block the fourth connecting port 407. At this time, the connecting tube 603 is inserted into the guide cavity 403. When nutrient solution is introduced into the guide cavity 403, the nutrient solution enters the culture dish 601 through the connecting tube 603, thus achieving the addition of nutrient solution. When air is introduced, the air enters the guide cavity 403, which can then enter the heat exchange chamber 505 for heat exchange and then be blown out into the incubator 101 for drying or humidification, thereby achieving a better temperature and humidity control effect and ensuring the survival rate of microorganisms.

[0035] Workflow: When using incubator 101 for direct cultivation, the culture medium and inoculum are directly fed into incubator 101. Then, sterile air is pumped into air heater 301 and heated to a suitable temperature as needed before being blown into insulation chamber 302, thus achieving better temperature control. Alternatively, air from insulation chamber 302 can be directly fed into rotating shaft 201 through a three-way valve, and then blown into incubator 101 directly from second connecting port 203, or directly from third connecting port 406 on stirring rod 402. The nutrient solution is blown out through the fourth connecting port 407, allowing the microorganisms to directly contact the air and preventing anaerobic respiration. Then, the liquid pump 107 is activated to extract the nutrient solution from the nutrient solution tank 106 and send it into the spray head 110, where it is sprayed out, allowing the nutrient solution to enter the incubator 101 for microbial cultivation. When the stirring rod 402 is needed for auxiliary addition, the electric valve 112 is activated to connect the connecting chamber 111 to the spray head 110, thus allowing the spray head to... The nutrient solution inside in incubator 110 can enter the rotating shaft 201 through the connecting cavity 111, and then enter the stirring rod 402, before being sprayed out directly. This allows the nutrient solution to be directly fed into the culture medium, thus feeding the microorganisms. Then, starting the motor 105 will stir the solution, ensuring the uniformity of the bacteria. When sampling is needed, the lid 104 can be moved upwards a certain distance, causing the plug 209 to slide upwards until the sampling port 212 connects with the incubator 101. Then, by manually pushing the piston 211 upwards via the guide rod, a sample can be taken. Some liquid culture medium can be directly discharged from the sampling port 212 and the discharge pipe 208 to achieve the sampling effect. After the culture is completed, for the liquid culture medium, the lid 104 can be moved upward a certain distance, so that the plug 209 slides upward away from the discharge pipe 208, and the liquid culture medium can be discharged from the discharge pipe 208. This can achieve the collection of microorganisms after the culture is completed, thereby improving work efficiency, reducing the residue of microorganisms and culture medium in the incubator 101, thereby reducing waste. After cleaning, high-temperature steam can be introduced for sterilization.When using petri dish 601 for culturing, place petri dish 601 in the clip holder 502, and secure it with the clip holder 502. After inoculating bacteria, cover with the sealing cap 602, and then clip the placement plate 501 onto the stirring rod 402. At this time, under the action of the pressing block 408, the pressing block 408 presses downward, causing the blocking block 405 to block the fourth connecting port 407. At this time, the connecting tube 603 is inserted into the guide cavity 403. When nutrient solution is introduced into the guide cavity 403, the nutrient solution enters the petri dish 601 through the connecting tube 603, thus achieving the addition of nutrient solution. When air is introduced, it enters the guide cavity 403, allowing it to enter the heat exchange chamber 505 for heat exchange, and then is blown out into the incubator 101 for drying or humidification, thereby achieving better temperature and humidity control and ensuring the survival rate of microorganisms.

[0036] In the description of this disclosure, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance. Furthermore, in the description of this disclosure, unless otherwise stated, "a plurality of" means two or more.

[0037] Any process or method description in the flowchart or otherwise herein can be understood as representing a module, segment, or portion of code comprising one or more executable instructions for implementing a particular logical function or process, and the scope of preferred embodiments of this disclosure includes additional implementations in which functions may be performed not in the order shown or discussed, including substantially simultaneously or in reverse order depending on the function involved, as will be understood by those skilled in the art to which embodiments of this disclosure pertain.

[0038] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of this disclosure. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0039] Although embodiments of the present disclosure have been shown and described above, it is to be understood that the above embodiments are exemplary and should not be construed as limiting the present disclosure. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present disclosure.

Claims

1. A constant temperature and humidity microbial inoculant cultivation device, characterized in that, include: An incubator (101) is provided with a cover (104) that slides on the incubator (101). A rotating shaft (201) is rotatably fitted inside the incubator (101). A stirring structure is installed around the rotating shaft (201). The stirring structure includes multiple stirring rods (402). A placement structure is installed inside the incubator (101). The placement structure includes multiple placement plates (501). The placement plates (501) are snapped and fixed to the stirring rods (402). A petri dish (601) is snapped and fixed on a placement plate (501). A connecting structure is installed between a stirring rod (402) and the petri dish (601). The connecting structure includes a connecting pipe (603), which is connected to the stirring rod (402) and the petri dish (601). A plug (405) is elastically fitted inside the stirring rod (402), and the stirring rod (402) is connected to a rotating shaft (201). The liquid delivery structure includes a nutrient solution tank (106) and a spray head (110). The spray head (110) is connected to a rotating shaft (201). A heating structure is installed at the bottom of the incubator (101). The heating structure includes an air heater (301). A discharge pipe (208) is installed at the bottom of the incubator (101). The discharge pipe (208) is connected to the rotating shaft (201). A sampling structure is installed at the bottom of the rotating shaft (201). The sampling structure includes a plug (209). Multiple sampling ports (212) are opened on the periphery of the plug (209).

2. The constant temperature and humidity microbial inoculant cultivation equipment according to claim 1, characterized in that, include: An electric slide rail (102) is fixed to the side of the incubator (101). A sliding frame (103) is fixed to the output end of the electric slide rail (102). The sliding frame (103) is fixedly connected to the cover (104). A motor (105) is fixed on the cover (104). The output end of the motor (105) is fixedly connected to the rotating shaft (201). The incubator (101) has a sealing groove (113) on its top, and a sealing block (114) is fixed on the underside of the cover (104). The sealing block (114) is located in the sealing groove (113).

3. The constant temperature and humidity microbial inoculant cultivation equipment according to claim 1, characterized in that, The liquid delivery structure also includes: A liquid pump (107) is fixed on the nutrient solution tank (106) and the nutrient solution tank (106) is fixed on the tank cover (104). The liquid pump (107) is fixed on the upper side of the nutrient solution tank (106), and the spray head (110) is fixed on the lower side of the tank cover (104). The liquid inlet end of the liquid pump (107) is fixed with an inlet pipe (108), and the liquid outlet end of the liquid pump (107) is fixed with an outlet pipe (109). The inlet pipe (108) is connected to the nutrient solution tank (106), and the outlet pipe (109) is connected to the spray head (110). The spray head (110) has an annular structure and a connecting cavity (111) is provided on the spray head (110). The connecting cavity (111) is connected to the spray head (110). An electric valve (112) is installed between the connecting cavity (111) and the spray head (110). The connecting cavity (111) is connected to the rotating shaft (201).

4. The constant temperature and humidity microbial inoculant cultivation equipment according to claim 3, characterized in that, The term includes: The rotating shaft (201) has a cavity (207) inside, and a plurality of first connecting ports (202) are provided on the side of the cavity (207). The first connecting ports (202) are connected to the connecting cavity (111). A plurality of second connecting ports (203) are provided on the periphery of the cavity (207). The second connecting ports (203) are connected to the incubator (101). A pressure valve (204) is installed in the second connecting port (203). The air pressure valve (204) includes a plug plate (205), a rotating shaft (206) is rotatably fitted inside the second connecting port (203), a torsion spring is fixed between the rotating shaft (206) and the second connecting port (203), and the rotating shaft (206) is fixedly connected to the plug plate (205).

5. The constant temperature and humidity microbial inoculant cultivation equipment according to claim 4, characterized in that, include: The incubator (101) is fixed with a base plate (214), which is fixedly connected to the discharge pipe (208). The discharge pipe (208) has a plug (209) located inside it. The plug (209) has a groove (210) inside it. The groove (210) is connected to the sampling port (212) and the discharge pipe (208). The groove (210) is a groove structure that is large at both ends and small in the middle. A piston (211) is slidably fitted inside the groove (210), and multiple first springs (213) are fixed between the piston (211) and the groove wall of the groove (210). The groove (210) is connected to the cavity (207).

6. The constant temperature and humidity microbial inoculant cultivation equipment according to claim 1, characterized in that, The heating structure also includes: A heat preservation chamber (302) is located around the incubator (101). An air pump is fixed on one side of the incubator (101). The air outlet of the air pump is connected to the air inlet of the air heater (301). The air outlet of the air heater (301) is connected to the heat preservation chamber (302). A three-way valve is installed at the bottom of the discharge pipe (208). The heat preservation chamber (302) is connected to the three-way valve. An air outlet is opened on one side of the heat preservation chamber (302). An air inlet pipe is installed on the top of the nutrient solution tank (106).

7. The constant temperature and humidity microbial inoculant cultivation equipment according to claim 1, characterized in that, include: Multiple connectors (401) are fixed on the rotating shaft (201). The connectors (401) are fixedly connected to the stirring rod (402). A flow guide cavity (403) is opened in the stirring rod (402). The flow guide cavity (403) is connected to the rotating shaft (201). A block (405) is slidably fitted in the flow guide cavity (403). Multiple second springs (404) are fixed between the block (405) and the cavity wall of the flow guide cavity (403). The stirring rod (402) has multiple third connecting ports (406) on its upper side and multiple fourth connecting ports (407) on its lower side. Both the third connecting ports (406) and the fourth connecting ports (407) are connected to the guide cavity (403). The placement plate (501) has a slot (503) on its lower side, and the stirring rod (402) is fixed in the slot (503).

8. The constant temperature and humidity microbial inoculant cultivation equipment according to claim 1, characterized in that, include: A pressing block (408) is fixed on the lower side of the placement plate (501), and a through groove (409) is provided on the stirring rod (402). The pressing block (408) passes through the through groove (409) and contacts the blocking block (405). The placement plate (501) is fixed with a clip bracket (502), the culture dish (601) is clipped and fixed inside the clip bracket (502), and the culture dish (601) is equipped with a sealing cap (602).

9. The constant temperature and humidity microbial inoculant cultivation equipment according to claim 1, characterized in that, include: The connecting tube (603) is fixedly connected to the sealing cap (602), and the connecting tube (603) is connected to the petri dish (601). One end of the connecting tube (603) is located inside the stirring rod (402).

10. The constant temperature and humidity microbial inoculant cultivation equipment according to claim 1, characterized in that, include: The placement plate (501) has a heat exchange chamber (505) inside. The heat exchange chamber (505) has multiple fifth communication ports (504) on its lower side. The fifth communication ports (504) are connected to the stirring rod (402). The heat exchange chamber (505) has multiple air outlets (506) on its side. An air pipe (507) is fixed on one side of the incubator (101).

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