A stand-alone embryo culture dish

Abstract

This invention provides an independent embryo culture dish, comprising a dish body, several culture chambers, and several waste liquid tanks. The culture chambers are arranged in a matrix on the dish body and connected to the dish body. A waste liquid tank is provided on one side of each row of culture chambers. The interior of each culture chamber is divided into an embryo culture layer, a covering oil layer, and an overflow control layer from bottom to top. A through-channel is provided between the overflow control layer and the waste liquid tank, and the overflow control layer and the waste liquid tank are connected by the through-channel. By setting an embryo culture layer, a covering oil layer, and an overflow control layer arranged from bottom to top in each independent culture chamber, this invention not only effectively ensures a stable environment for the embryo culture medium, but also achieves automatic discharge of excess culture oil through the through-channel structure between the overflow control layer and the waste liquid tank, avoiding culture environment disorder and cross-contamination caused by excessive oil layer thickness.

Description

Technical Field

[0001] This utility model relates to an embryo culture dish, specifically an independent embryo culture dish, and belongs to the field of embryo culture technology. Background Technology

[0002] In in vitro embryo culture, traditional culture dishes typically employ multiple wells arranged side-by-side. Each well contains a specific amount of embryo culture medium and covering oil to maintain evaporation balance and provide a sterile environment. However, in practice, inaccurate oil volume control or improper operation can easily lead to excessive oil overflowing onto the outside of the culture dish or adjacent wells. This not only affects the stability of the culture environment but may also cause cross-contamination between samples. Furthermore, some culture dish designs lack effective wastewater guidance structures, making it difficult to promptly drain excess oil. This necessitates frequent operator intervention, increasing the possibility of human error and experimental complexity. Utility Model Content

[0003] Based on the above background, the purpose of this utility model is to provide an independent embryo culture dish with automatic overflow control and waste liquid guidance, thereby solving the problems described in the background art.

[0004] To achieve the above-mentioned objectives, this utility model provides the following technical solution:

[0005] An independent embryo culture dish includes a dish body, several culture chambers, and several waste liquid tanks. The culture chambers are arranged in a matrix on the dish body and connected to the dish body. A waste liquid tank is provided on one side of each row of culture chambers.

[0006] The interior of the culture chamber is divided into an embryo culture layer, a covering oil layer, and an overflow control layer from bottom to top. A through channel is provided between the overflow control layer and the waste liquid tank, and the overflow control layer and the waste liquid tank are connected by the through channel. The height of the waste liquid tank is lower than the height of the overflow control layer, and the bottom of the through channel has a sloping structure from the overflow control layer toward the waste liquid tank.

[0007] Preferably, the freestanding embryo culture dish also includes a lid, which is located on the top outer side of the dish body. The lid is detachably connected to the dish body, and the outer edge of the lid is provided with several protrusions, which are integral with the lid.

[0008] Preferably, baffles are provided on both sides of the through groove, and the baffles are connected to the through groove and the dish body.

[0009] Preferably, several rinsing chambers are provided at both ends of the upper part of the dish.

[0010] Preferably, the protrusions are distributed at equal intervals on the outer edge of the lid.

[0011] Preferably, one end of the dish body extends outward with a first marking area, which is connected to the dish body.

[0012] Preferably, a second marking area is provided on the top of the lid.

[0013] Preferably, the first marking area is provided with a frosted layer.

[0014] Compared with the prior art, the present invention has the following advantages:

[0015] This invention relates to an independent embryo culture dish, which, by setting up an embryo culture layer, a covering oil layer, and an overflow control layer arranged sequentially from bottom to top in each independent culture chamber, not only effectively ensures a stable environment for the embryo culture medium, but also achieves automatic discharge of excess culture oil through the through-channel structure between the overflow control layer and the waste liquid tank, thus avoiding culture environment disorder and cross-contamination caused by excessive oil layer. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the structure of the culture chamber of this utility model on the dish body;

[0018] Figure 2 This is a cross-sectional view of the vessel body of this utility model;

[0019] Figure 3 yes Figure 1 Enlarged schematic diagram of section A in the middle;

[0020] Figure 4 This is a schematic diagram of the lid structure of this utility model.

[0021] In the diagram: 1. Dish body; 2. Culture chamber; 3. Waste liquid tank; 4. Embryo culture layer; 5. Covering oil layer; 6. Overflow control layer; 7. Through channel; 8. Sloping structure; 9. Dish lid; 10. Protrusion; 11. Baffle; 12. Rinse chamber; 13. First identification area; 14. Second identification area. Detailed Implementation

[0022] The technical solution of this utility model will be further described in detail below through specific embodiments and in conjunction with the accompanying drawings. It should be understood that the implementation of this utility model is not limited to the following embodiments, and any modifications and / or alterations made to this utility model will fall within the protection scope of this utility model.

[0023] In this invention, unless otherwise specified, all parts and percentages are by weight, and the equipment and raw materials used are commercially available or commonly used in the field. Unless otherwise specified, the methods in the following embodiments are conventional methods in the field. Unless otherwise specified, the components or equipment in the following embodiments are general standard parts or components known to those skilled in the art, and their structures and principles can be learned by those skilled in the art through technical manuals or conventional experimental methods.

[0024] The embodiments of the present invention will be described in detail below with reference to the accompanying drawings. In the following detailed description, many specific details are set forth to facilitate explanation and provide a comprehensive understanding of the embodiments of the present invention. However, one or more embodiments may be practiced by those skilled in the art without these specific details.

[0025] like Figure 1 As shown, an independent embryo culture dish includes a dish body 1, several culture chambers 2, and several waste liquid tanks 3. The culture chambers 2 are arranged in a matrix on the upper surface of the dish body 1, and are all integrally formed and connected to the dish body 1 to form an independent and closed culture unit.

[0026] Each row of culture chambers 2 has a corresponding waste liquid tank 3 on one side. The waste liquid tank 3 extends in a long and narrow trough shape and is located near the outer edge of the corresponding row of culture chambers 2. The height of the waste liquid tank 3 is lower than the overflow control layer 6 inside the corresponding culture chamber 2, thereby creating a height difference that automatically guides the overflow of oil.

[0027] like Figure 2 As shown, the interior of each culture chamber 2 is divided into three layers from bottom to top: the bottom layer is the embryo culture layer 4, in which embryo culture medium and embryos to be cultured are added; the middle layer is the oil covering layer 5, on which a certain thickness of sterile culture oil is added above the culture medium to isolate external air and maintain the stability of the liquid level; the top layer is the overflow control layer 6, which is used to guide excess culture oil into the external waste liquid tank 3.

[0028] like Figure 3As shown, a through channel 7 is provided between the overflow control layer 6 and the waste liquid tank 3. The through channel 7 is a narrow, horizontally opened slot that connects the overflow control layer 6 of the culture chamber 2 to the external waste liquid tank 3. To prevent direct spillage and leakage of liquid, the bottom of the through channel 7 has a slope structure 8 that gradually decreases from the overflow control layer 6 towards the waste liquid tank 3, forming a liquid drainage channel. When the volume of the intermediate culture oil is too large, the oil will rise in the overflow control layer 6 to the through channel 7 and flow into the waste liquid tank 3 along the slope structure 8 of the through channel 7, effectively avoiding cross-over overflow between the culture chambers 2. Baffles 11 are also provided on both sides of the through channel 7 and connected to it to prevent unnecessary overflow caused by external airflow or oil fluctuations during operation, and to prevent external liquids or particles from entering the culture chamber 2 in reverse.

[0029] Several rinsing chambers 12 are also provided at both ends of the upper part of the dish body 1 to hold rinsing fluid for subsequent embryo rinsing treatment.

[0030] like Figure 4 As shown, for ease of use, this freestanding embryo culture dish also includes a lid 9 located on the outer side of the top of the dish body 1. The lid 9 is detachably mounted from the dish body 1, and its outer edge is evenly provided with several protrusions 10 integrally formed with the lid 9, which facilitates the user's grip or quick positioning and installation. The protrusions 10 are preferably distributed at equal intervals on the outer edge of the lid 9.

[0031] The dish 1 has a first marking area 13 extending outward from one end. The first marking area 13 can be used to mark information such as the dish number, date, and experimental batch of the dish 1. In addition, the surface of the first marking area 13 has a frosted layer to facilitate writing and prevent the writing from being erased.

[0032] In addition, a second marking area 14 is provided on the top of the dish lid 9 to facilitate marking of each culture chamber 2.

[0033] The implementation principle of this utility model of an independent embryo culture dish is as follows:

[0034] By setting multiple independent culture chambers 2 on the dish body 1, each culture chamber 2 has an embryo culture layer 4, a covering oil layer 5, and an overflow control layer 6 arranged from bottom to top, thus achieving independent closed control of the culture environment for a single embryo. During operation, a predetermined volume of culture medium and embryos are added to each culture chamber 2, and then the culture oil is used to cover it, which inhibits evaporation and maintains a sterile state.

[0035] When an excessive amount of culture oil is added, the excess oil will rise to the overflow control layer 6 and automatically flow into the lower waste liquid tank 3 through the channel 7 set between it and the waste liquid tank 3, thereby preventing the oil from overflowing into the adjacent culture chamber 2 and effectively preventing cross-contamination between samples.

[0036] The bottom of the channel 7 is equipped with a ramp structure 8, which can guide the oil to flow quickly to the waste liquid tank 3 when there is excess liquid. At the same time, in conjunction with the baffles 11 on both sides, the oil flow channel is further stabilized to avoid external disturbances that could cause liquid backflow or side leakage.

[0037] This article uses specific examples to illustrate the principles and implementation methods of this utility model. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of this utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made to this utility model without departing from the principles of this utility model, and these improvements and modifications also fall within the protection scope of the claims of this utility model.

Claims

1. A stand-alone embryo culture dish, characterized by: The freestanding embryo culture dish includes a dish body (1), several culture chambers (2) and several waste liquid tanks (3). Several culture chambers (2) are arranged in a matrix on the dish body (1) and the culture chambers (2) are connected to the dish body (1). A waste liquid tank (3) is opened on one side of each row of culture chambers (2). The interior of the culture chamber (2) is divided into an embryo culture layer (4), an oil covering layer (5), and an overflow control layer (6) from bottom to top. A through channel (7) is provided between the overflow control layer (6) and the waste liquid tank (3). The overflow control layer (6) and the waste liquid tank (3) are connected through the through channel (7). The height of the waste liquid tank (3) is lower than the height of the overflow control layer (6). The bottom of the through channel (7) is provided with a slope structure (8) from the overflow control layer (6) toward the waste liquid tank (3).

2. The freestanding embryo culture dish according to claim 1, characterized in that: The independent embryo culture dish also includes a lid (9), which is located on the top outer side of the dish body (1). The lid (9) and the dish body (1) are detachably connected. The outer edge of the lid (9) is provided with several protrusions (10), and the protrusions (10) and the lid (9) are an integral structure.

3. The freestanding embryo culture dish according to claim 1, characterized in that: Both sides of the through groove (7) are provided with baffles (11), and the baffles (11) are connected to the through groove (7) and the dish body (1).

4. The freestanding embryo culture dish according to claim 1, characterized in that: Several rinsing chambers (12) are provided at both ends of the upper part of the dish body (1).

5. The freestanding embryo culture dish according to claim 2, characterized in that: Several of the protrusions (10) are distributed at equal intervals on the outer edge of the lid (9).

6. The freestanding embryo culture dish according to claim 4, characterized in that: One end of the dish (1) extends outward to have a first marking area (13), which is connected to the dish (1).

7. The freestanding embryo culture dish according to claim 5, characterized in that: A second marking area (14) is provided on the top of the lid (9).

8. The freestanding embryo culture dish according to claim 6, characterized in that: The first marking area (13) is provided with a frosted layer.

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