Application of plant growth regulator in regulation of cotton growth

Abstract

The invention relates to the technical field of application of plant growth regulators to regulation of cotton growth, in particular to application of a plant growth regulator containing chlormequat chloride and uniconazole to regulation of cotton growth, 1000 parts by weight of the plant growth regulator comprises the following components in parts by weight: 70-490 parts of chlormequat chloride, 10-70 parts of uniconazole, 50-250 parts of auxiliaries and the balance of water, wherein the weight ratio of chlormequat chloride to uniconazole is 7: 1. The plant growth regulator disclosed by the invention can effectively control vigorous growth, increase thickness, inhibit height and shrink sections, so that early peach setting, more flowers, more peaches and less shedding are realized, and effective substances for vegetative growth are transferred to reproductive growth, so that the purposes of high yield and quality improvement of cotton are achieved, and the plant growth regulator is safe to cotton, safe to the environment and small in residue.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, specifically relating to the application of a plant growth regulator containing chlormequat chloride and uniconazole in regulating cotton growth. Background Technology

[0002] Chlormequat chloride is an excellent plant growth regulator that can be used on crops such as wheat, rice, cotton, tobacco, corn, and tomatoes. It inhibits crop cell elongation but does not inhibit cell division, making plants shorter, stems thicker, and leaves greener. It can make crops more drought-resistant and flood-resistant, prevent excessive growth and lodging, resist salinity and alkalinity, prevent cotton boll drop, and increase potato tuber size.

[0003] Uniconazole is a broad-spectrum, highly effective plant growth regulator with both fungicidal and herbicidal effects. It is an inhibitor of gibberellin synthesis. It controls vegetative growth, inhibits cell elongation, shortens internodes, dwarfs plants, promotes lateral bud growth and flower bud formation, and enhances stress resistance. Its activity is 6-10 times higher than paclobutrazol, but its residue in the soil is only 1 / 10 that of paclobutrazol, thus having minimal impact on subsequent crops. It can be absorbed through seeds, roots, buds, and leaves, and translocated between organs, but leaf absorption and outward translocation are relatively limited. It exhibits significant acrotropism. It is suitable for rice and wheat, increasing tillering, controlling plant height, and improving lodging resistance. It is used to control vegetative growth in fruit trees and to shape ornamental plants, promoting flower bud differentiation and increased flowering.

[0004] Besides controlling pests and diseases, increasing cotton yield through the use of fertilizers or plant growth regulators is a crucial issue for cotton farmers. However, the proper use of plant growth regulators to increase yield is a skill; improper application can lead to reduced yields or even worse, wilting and boll shedding. Through experimentation, the applicant discovered that mixing chlormequat chloride and tebuconazole (with different mechanisms of action) in a 7:1 weight ratio to create a suspension can effectively control excessive growth, increase thickening, suppress dwarfing, and shorten internodes, promoting earlier boll setting, more flowers and bolls, and less boll shedding. This transfers the effective substances for vegetative growth to reproductive growth, thereby achieving high cotton yields. Summary of the Invention

[0005] The purpose of this invention is to provide an application of a plant growth regulator in regulating cotton growth. The plant growth regulator has a good effect on increasing cotton yield and improving quality, and its effect is significantly better than that of the control single agent. It also has a synergistic effect and is safe for the environment and cotton.

[0006] The technical solution of this invention is:

[0007] The application of a plant growth regulator in regulating cotton growth is characterized in that: the effective active ingredients of the plant growth regulator are chlormequat chloride and uniconazole, which are added with adjuvants and water to form a suspension, wherein the weight ratio of chlormequat chloride to uniconazole is 7:1.

[0008] The plant growth regulator, based on 1000 parts by weight, comprises the following components in parts by weight: 70-490 parts of chlormequat chloride, 10-70 parts of uniconazole, 50-250 parts of adjuvants, and the remainder being water.

[0009] Further, based on 1000 parts by weight, it includes the following components in parts by weight: 210-420 parts of chlormequat chloride, 30-60 parts of uniconazole, 80-180 parts of adjuvants, and water as the balance.

[0010] Furthermore, the total weight of chlormequat chloride and uniconazole in the regulator is 80–560 g / L, with an optional total weight of 240–480 g / L, and an optional total weight of 400 g / L.

[0011] Furthermore, the additives are selected from one or more of the following: dispersants, wetting agents, antifreeze agents, preservatives, defoamers, pH adjusters, and thickeners.

[0012] Further, the dispersant is selected from one or more of alkyl naphthalene sulfonate, alkyl naphthalene sulfonate formaldehyde polymer, lignin sulfonate, fatty alcohol polyoxyethylene ether phosphate, polycarboxylate, comb-type polycarboxylate, EO-PO block copolymer, and phenethylphenol polyoxyethylene ether phosphate; the wetting agent is selected from one or more of alkyl sulfonate, alkyl naphthalene sulfonate, fatty alcohol polyoxyethylene ether, alkyl glycoside, trisiloxane polyoxyethylene ether, polyarylphenol polyoxyethylene ether, and castor oil polyoxyethylene ether; the antifreeze agent is selected from ethyl phenol polyoxyethylene ether. The ingredients are selected from one or more of the following: glycol, propylene glycol, glycerol, polyethylene glycol, allantoin, and sorbitol; the preservative is benzoic acid and its sodium salt, isothiazolinone, sorbic acid and its sodium or potassium salt; the defoamer is a silicone defoamer, a C10-20 saturated fatty acid compound, or a C8-10 fatty alcohol; the thickener is selected from one or more of xanthan gum, polyvinyl alcohol, magnesium aluminum silicate, sodium carboxymethyl cellulose, sodium alginate, guar gum, and silica; and the pH adjuster is selected from one or more of citric acid and glacial acetic acid.

[0013] Further, based on 1000 parts by weight, it comprises: 10-80 parts of dispersant, optionally 20-60 parts; 5-40 parts of wetting agent, optionally 8-30 parts; 10-70 parts of antifreeze, optionally 20-60 parts; 0-10 parts of preservative, optionally 1-8 parts; 0.1-10 parts of defoamer, optionally 0.1-5 parts; 0-20 parts of pH adjuster, optionally 1-12 parts; and 0.5-20 parts of thickener, optionally 1-15 parts.

[0014] Furthermore, the application of regulators in regulating cotton growth and improving cotton quality may optionally include the application of regulators in inhibiting plant height, increasing fruiting branches, increasing the number of bolls, increasing yield, and improving cotton quality.

[0015] Furthermore, the regulator should be applied once at the initial flowering stage of cotton and once after topping, using 600-750 liters of water per hectare and 50-120 mg / kg of effective active ingredient. The optional application rate is 57.1-66.7 mg / kg, and the optional application rate is 66.7 mg / kg.

[0016] The advantages of this invention are: (1) Compared with single agents, it has a good synergistic effect on cotton plant height inhibition, fruit branch increase, boll number increase and yield increase; (2) It significantly improves cotton quality, and can improve cotton uniformity, fiber length and micronaire value; (3) It is safe for cotton, will not cause phytotoxicity and is safe for subsequent crops. Detailed Implementation

[0017] The present invention will be further described below with reference to the embodiments. The components are composed of the following amounts based on 1000 parts by weight, but the present invention is not limited thereto.

[0018] Application Example 1

[0019] Example 1: 400 g / L chlormequat chloride·uniconazole suspension

[0020] 350 parts of chlormequat chloride, 50 parts of uniconazole, 25 parts of polycarboxylate, 12 parts of fatty alcohol polyoxyethylene ether, 40 parts of ethylene glycol, 3 parts of benzoic acid and its sodium salt, 2 parts of silicone defoamer, 8 parts of magnesium aluminum silicate, 2 parts of citric acid, and water to balance.

[0021] Example 2: 400 g / L chlormequat chloride·uniconazole suspension

[0022] 350 parts of chlormequat chloride, 50 parts of uniconazole, 10 parts of alkylnaphthalene sulfonate formaldehyde polymer, 15 parts of lignin sulfonate, 20 parts of alkyl glycoside, 40 parts of propylene glycol, 2 parts of sorbic acid and its sodium salt, 3 parts of C8-10 fatty alcohols, 2 parts of xanthic acid gum, 3 parts of citric acid, and water to balance.

[0023] Example 3: 400 g / L chlormequat chloride·uniconazole suspension

[0024] 350 parts of chlormequat chloride, 50 parts of uniconazole, 40 parts of fatty alcohol polyoxyethylene ether phosphate, 10 parts of trisiloxane polyoxyethylene ether, 50 parts of glycerol, 4 parts of sodium benzoate, 2 parts of silicone defoamer, 8 parts of silica, 7 parts of glacial acetic acid, and water to balance.

[0025] Example 4: 240 g / L chlormequat chloride·uniconazole suspension

[0026] 210 parts of chlormequat chloride, 30 parts of uniconazole, 30 parts of phenylethylphenol polyoxyethylene ether phosphate, 10 parts of alkyl sulfonate, 40 parts of ethylene glycol, 1 part of isothiazolinone, 2 parts of C8-10 fatty alcohols, 15 parts of silica, 1 part of glacial acetic acid, and water to balance.

[0027] Example 5: 320 g / L chlormequat chloride·uniconazole suspension

[0028] 280 parts of chlormequat chloride, 40 parts of uniconazole, 40 parts of lignin sulfonate, 5 parts of comb-type polycarboxylate, 18 parts of polyarylphenol polyoxyethylene ether, 50 parts of sorbitol, 2 parts of benzoic acid and its sodium salt, 5 parts of C10-20 saturated fatty acid compounds, 3 parts of polyvinyl alcohol, 4 parts of citric acid, and water to balance.

[0029] Example 6: 480 g / L chlormequat chloride·uniconazole suspension

[0030] 420 parts of chlormequat chloride, 60 parts of uniconazole, 15 parts of comb-type polycarboxylate, 25 parts of EO-PO block copolymer, 10 parts of fatty alcohol polyoxyethylene ether, 10 parts of alkyl naphthalene sulfonate, 40 parts of ethylene glycol, 4 parts of isothiazolinone, 2 parts of silicone defoamer, 5 parts of citric acid, and water to balance.

[0031] Control agent 1: 80% chlormequat chloride soluble powder, commercially available.

[0032] Control agent 2.5% uniconazole wettable powder, commercially available.

[0033] Implementation Example 2:

[0034] Report on the Indoor Combined Toxicity Assay Test of the Mixture of Chlormequat Chloride and Uniconazole for Regulating Cotton Growth

[0035] Test target: cotton, grown to the initial flowering stage.

[0036] Experimental method: Pot planting method

[0037] Test basis: Refer to NY / T 2061.2-2011 and NY / T 2061.5-2016

[0038] 1. Experimental Objective

[0039] Using chlormequat chloride and uniconazole as test agents, the optimal ratio was screened by indoor combined treatment after spraying to determine the formula. This provides a basis for formula screening for the rational mixing of the two agents and the development of effective agents for regulating cotton growth. The test results are reported as follows.

[0040] 2. Cultivation conditions

[0041] The target is cotton, and the cultivation temperature (25-30℃), light (light-to-dark ratio 14:10), and humidity (65-75%) can be automatically controlled.

[0042] 3 Experimental Design

[0043] 3.1 Reagents

[0044] 98% chlormequat chloride technical grade, provided by the applicant;

[0045] 90% of the uniconazole technical grade was provided by the applicant;

[0046] Solvent: N,N-dimethylformamide;

[0047] Emulsifier: Tween 80 (0.1% aqueous solution).

[0048] 3.2 Test Treatment

[0049] 3.2.1 Dosage setting

[0050] Chlormequat chloride and uniconazole were respectively prepared with N,N-dimethylformamide to a concentration of 1×10⁻⁶. 4 mg / L stock solution.

[0051] Based on the individual activity of the two agents and the characteristics of indoor assays, and on the basis of the preliminary test, the dosages of chlormequat chloride and uniconazole were set as different combinations, as shown in Table 1, for a total of 15 groups.

[0052] An aqueous solution containing the same solvent and emulsifier but without any reagents was used as a blank control for in-house bioassays.

[0053] Table 1 Experimental reagent dosing design

[0054]

[0055] 3.2.2 Experimental Repetition

[0056] Each concentration of the experimental agent was divided into 4 replicates, with 20 pots per replicate and 1 cotton plant per pot.

[0057] 3.3 Processing Method

[0058] The experiment involved applying the pesticide once at the initial flowering stage and once after topping, for a total of two applications. One cotton plant was placed in each pot, and the pesticide treatment began when the cotton reached the initial flowering stage.

[0059] 4. Data Survey and Statistical Analysis

[0060] 4.1 Results, Survey Time and Methods

[0061] Twenty days after the first treatment, the absolute value (numerical measurement) survey method was used, and 10 pots were randomly selected from each replicate to investigate the plant height of cotton.

[0062] Forty days after the second application of the drug, ten cotton bolls were randomly selected from each replicate and dried until the seed cotton moisture content was below 12% before the weight of a single cotton boll was investigated.

[0063] 4.2 Data Statistical Analysis

[0064] Based on the measurement results, the inhibition rate of cotton plant height and the growth rate of single boll weight were calculated for each treatment.

[0065] The theoretical inhibition rate (growth rate) E0 (E0=X+YX*Y / 100) of each treatment combination was calculated using the Gowing method, and then compared with the measured inhibition rate (growth rate) (E) to evaluate the combined effect of the two treatments on cotton.

[0066] When the E-E0 value is greater than 10%, it indicates a synergistic effect; when the E-E0 value is less than -10%, it indicates an antagonistic effect; and when the E-E0 value is between -10% and 10%, it indicates an additive effect. The optimal ratio is determined based on factors such as actual efficacy, the characteristics of the two drugs, and the balance of the formulation.

[0067] In the formula, X is the cotton plant height inhibition rate (single boll weight growth rate) when the dosage of chlormequat chloride is P; Y is the cotton plant height inhibition rate (single boll weight growth rate) when the dosage of clopidogrel is Q.

[0068] 5 Results and Analysis

[0069] Table 2. Indoor synergistic effect determination of chlormequat chloride and paclobutrazol in cotton (Gowing method)

[0070]

[0071]

[0072] Table 2 shows that the combined use of chlormequat chloride and uniconazole has a certain inhibitory effect on cotton plant height and a good effect on increasing single boll weight. The E-E0 values ​​of plant height inhibition rate for each treatment were positive, and the efficacy showed different increasing trends. Among them, the treatment with a chlormequat chloride to uniconazole ratio of 7:1 (42 mg / kg + 6 mg / kg) had the highest E-E0 value for cotton plant height inhibition rate at 10.09%, indicating a synergistic effect.

[0073] The combined use of chlormequat chloride and uniconazole resulted in positive E-E0 values ​​for the increase in cotton boll weight, with different trends in efficacy. Among them, the treatment with a 7:1 ratio of chlormequat chloride to uniconazole (42 mg / kg + 6 mg / kg) showed the largest E-E0 value for the increase in cotton boll weight at 6.70%, indicating an additive effect.

[0074] Observations during the experiment showed that the two agents and their respective dosage ratios were safe for the growth of the experimental cotton and caused no phytotoxicity.

[0075] Application Example 3: Field efficacy experiment of 400 g / L chlormequat chloride·uniconazole suspension (Examples 1-3) in regulating cotton growth.

[0076] 1. Experimental Objective

[0077] To verify the regulatory effect, appropriate timing of application, recommended dosage, and safety of the 400 g / L chlormequat chloride-uniconazole suspension developed by the applicant on cotton growth, and to provide a basis for the registration of the product.

[0078] 2. Experiments and Methods

[0079] 2.1 Experimental crop: cotton, with a planting density of 2000 plants / mu to 3500 plants / mu.

[0080] 2.2 Usage capacity: Spraying method, water consumption 600-750 liters / hectare.

[0081] 2.3 Experimental reagent treatment

[0082] Table 3 Treatment of test reagents

[0083]

[0084] 2.4 Application Method

[0085] The experiment involved two applications of the pesticide, one at the initial flowering stage of the cotton and the other after topping.

[0086] 2.5 Survey Methods

[0087] Before application of the pesticide, 10 representative cotton plants were selected from each treatment area and tagged as survey points. Twenty days after the last application, cotton plant height, number of fruiting branches, and number of bolls were surveyed. Sample bolls were collected before frost, dried for 7 days, and then the weight of each boll was measured at the different treatment points. During the main quality tests, the seed cotton yield was surveyed at harvest.

[0088] Safety surveys were conducted three times, three days after each administration.

[0089] 2.6 Calculation and Analysis Methods

[0090]

[0091]

[0092]

[0093]

[0094] 3 Results and Analysis

[0095] Table 4. Effects of 400 g / L chlormequat chloride·uniconazole suspension concentrate and control agent on cotton yield

[0096]

[0097] The effects of the tested agent 400 g / L chlormequat chloride·uniconazole suspension on cotton growth and yield are shown in Table 4. The number of cotton fruiting branches, the number of bolls, and the yield were significantly increased in the treatments of chlormequat chloride·uniconazole suspension at 57.1 mg / kg and 66.7 mg / kg, respectively. The compound effect was significant and showed extremely significant differences compared with the control single agent.

[0098] Table 5. Results of the experiment on the effects of 400 g / L chlormequat chloride·uniconazole suspension and control on cotton quality.

[0099]

[0100]

[0101] The main quality of cotton was tested, and the main quality of the cotton treated with 400 g / L chlormequat chloride·uniconazole suspension was significantly better than that of the blank control. The quality of the cotton treated with the test agent was better than that of the control single agent, indicating that the dosage of the test agent can improve the quality of cotton.

[0102] Application Example 4: Field efficacy experiment of chlormequat chloride·uniconazole suspension (Examples 4-6) in regulating cotton growth

[0103] 1. Experimental Objective

[0104] To verify the regulatory effect, appropriate timing of application, recommended dosage, and safety of the chlormequat chloride-uniconazole suspension (Examples 4-6) developed by the applicant on cotton growth, and to provide a basis for the registration of the product.

[0105] 2. Experiments and Methods

[0106] 2.1 Experimental crop: cotton.

[0107] 2.2 Usage capacity: Spraying method, water consumption 600-750 liters / hectare.

[0108] 2.3 Experimental reagent treatment

[0109] Table 6 Treatment of Test Reagents

[0110]

[0111]

[0112] 2.4 Application Method

[0113] The experiment involved two applications of the pesticide, one at the initial flowering stage of the cotton and the other after topping.

[0114] 2.5 Survey Methods

[0115] Before application of the pesticide, 10 representative cotton plants were selected from each treatment area and tagged as survey points. Twenty days after the last application, cotton plant height, number of fruiting branches, and number of bolls were surveyed. Sample bolls were collected before frost, dried for 7 days, and then the weight per boll was measured based on the difference between the sample points. At harvest, the seed cotton yield was surveyed.

[0116] Safety surveys were conducted once before application and once three days after each application, for a total of three safety surveys.

[0117] 2.6 Calculation and Analysis Methods

[0118]

[0119]

[0120]

[0121]

[0122] 3 Results and Analysis

[0123] Table 7. Effects of chlormequat chloride·uniconazole suspension and control single agent on cotton yield.

[0124]

[0125] The effects of the tested agents, chlormequat chloride·uniconazole suspension, on cotton growth and yield are shown in Table 7. The treatments of chlormequat chloride·uniconazole suspension at 57.1 mg / kg and 66.7 mg / kg significantly increased the number of fruiting branches, the number of bolls, and the yield of cotton. The compound treatment showed significant synergistic effects and was highly significantly different from the control agent. Within the experimental range, it controlled the excessive growth of cotton plants to varying degrees, increased the weight of single bolls, and improved the quality of cotton.

[0126] Application Example 5: Safety Experiment of Different Concentrations of Chlormequat Chloride·Uniconazole Suspension on Cotton Growth (Examples 1-6)

[0127] 1. Experimental Objective

[0128] To verify the safety of the chlormequat chloride-uniconazole suspension (Examples 1-6) developed by the applicant on cotton growth, and to provide a basis for the registration of the product.

[0129] 2. Experiments and Methods

[0130] 2.1 Experimental crop: cotton.

[0131] 2.2 Usage capacity: Spraying method, water consumption 600-750 liters / hectare.

[0132] 2.3 Experimental reagent treatment

[0133] Table 8 Treatment of Test Reagents

[0134]

[0135] 2.4 Application Method

[0136] The experiment involved two applications of the pesticide, one at the initial flowering stage of the cotton and the other after topping.

[0137] 2.5 Survey Methods

[0138] A survey was conducted once before application of the pesticide, and then again one day and three days after each application, for a total of four surveys. The main focus of the survey was to investigate whether any pesticide damage occurred, and to record the type and severity of the damage, such as wilting, chlorosis, and deformity.

[0139] 3 Results and Analysis

[0140] Table 9. Effects of different concentrations of test and control pesticides on cotton growth safety.

[0141]

[0142] It can be seen that the tested agent, chlormequat chloride·uniconazole suspension, is safe for cotton at all dosages and does not cause phytotoxicity. In contrast, the single agent, 80% chlormequat chloride soluble powder and 5% uniconazole wettable powder, both caused phytotoxicity to varying degrees at high dosages.

[0143] The above description, in conjunction with specific preferred embodiments, provides a further detailed explanation of the present invention. It should not be construed that the specific implementation of the present invention is limited to these descriptions. For those skilled in the art, various simple deductions or substitutions can be made without departing from the concept of the present invention, and all such modifications and substitutions should be considered within the scope of protection of the present invention.

Claims

1. The application of a plant growth regulator in regulating cotton growth, characterized in that: The effective active ingredients of the plant growth regulator are chlormequat chloride and uniconazole. They are added with adjuvants and water to form a suspension, wherein the weight ratio of chlormequat chloride to uniconazole is 7:

1. The plant growth regulator, based on 1000 parts by weight, comprises the following components in parts by weight: 70-490 parts of chlormequat chloride, 10-70 parts of uniconazole, 50-250 parts of adjuvants, and the remainder being water.

2. The application according to claim 1, characterized in that: The plant growth regulator, based on 1000 parts by weight, comprises the following components in parts by weight: 210-420 parts of chlormequat chloride, 30-60 parts of uniconazole, 80-180 parts of adjuvants, and water as the remainder.

3. The application according to claim 1 or 2, characterized in that: The total weight of chlormequat chloride and uniconazole in the regulator is 80~560 g / L, with an optional total weight of 240~480 g / L and an optional total weight of 400 g / L.

4. The application according to claim 1, characterized in that: The additives are selected from one or more of the following: dispersants, wetting agents, antifreeze agents, preservatives, defoamers, pH adjusters, and thickeners.

5. The application according to claim 4, characterized in that: The regulator contains 10 to 80 parts of dispersant, optionally 20 to 60 parts, per 1000 parts by weight; The wetting agent accounts for 5 to 40 parts by weight of the regulator, and optionally 8 to 30 parts. The antifreeze content as a percentage of the regulator is 10-70 parts by weight, and optionally 20-60 parts by weight; The preservative accounts for 0 to 10 parts by weight of the regulator, and optionally 1 to 8 parts; The defoamer accounts for 0.1 to 10 parts by weight of the regulator, and optionally 0.1 to 5 parts. The pH adjuster accounts for 0 to 20 parts by weight of the adjuster, and optionally 1 to 12 parts; The thickener accounts for 0.5 to 20 parts by weight of the regulator, and optionally 1 to 15 parts.

6. The application according to any one of claims 1 to 5, characterized in that: Application of growth regulators in regulating cotton growth and improving cotton quality.

7. The application according to claim 6, characterized in that: The application of growth regulators in cotton to suppress plant height, increase fruiting branches, increase boll number, increase yield, and improve cotton quality.

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