Enzyme-containing anti-inflammatory composition for pet skin and hair follicle health

Abstract

The invention discloses an enzyme-containing anti-inflammatory composition for pet skin and hair follicle health. The enzyme-containing anti-inflammatory composition comprises an enzyme preparation, a hair follicle maintenance agent, a functional auxiliary agent and a carrier raw material. The core innovation lies in that a hair follicle targeted enzymolysis technology is adopted, and a specific enzyme modified by polyethylene glycol can efficiently dredge hair follicles; the anti-inflammatory and hair follicle repairing dual effects are achieved through anti-inflammatory-maintenance synergistic matching and adding sequence optimization; the damaged barrier is rapidly repaired by combining a composite barrier repairing agent and a gradient permeation process; enzyme activity and mildness are guaranteed by adopting a microcapsule embedding and low-temperature preparation technology. The composition can specifically solve the problems of hair follicle blockage, inflammation and unhairing, is suitable for pets of all ages and skin types, and is high in practicability.

Description

Technical Field

[0001] This invention belongs to the field of pet skin and hair follicle care technology, specifically relating to an enzyme-containing anti-inflammatory composition for the health of pet skin and hair follicles. Background Technology

[0002] Pet skin and hair follicle diseases are frequent, with core causes including the accumulation of sebum and keratin debris within the hair follicles, pathogenic bacterial infections, damaged skin barriers, and nutritional deficiencies. Existing pet skin care products often suffer from single-faceted limitations: focusing only on cleansing or anti-inflammation, lacking a synergistic effect of "unblocking-anti-inflammatory-nourishing-repair"; enzyme preparations suffer from poor targeting, easy inactivation, and potential damage to hair follicle stem cells; plant extracts and nutrients do not fully realize their full potential, and some products exhibit unstable effects due to insufficient raw material purity and improper processing; furthermore, traditional products often have strong irritant properties and are unsuitable for pets with sensitive skin. In addition, current manufacturing processes lack effective protection of enzyme activity, and barrier repair ingredients have weak penetration, making it difficult to quickly repair damaged tissue. These problems prevent existing products from addressing the root causes of pet hair follicle and skin health issues, necessitating the development of a highly targeted, synergistic, gentle, stable, and multi-functional enzyme-containing anti-inflammatory composition to meet the actual needs of pet skin and hair follicle care. Summary of the Invention

[0003] To address the aforementioned problems, this invention proposes an enzyme-containing anti-inflammatory composition for pet skin and hair follicle health. This composition combines multiple functions, including targeted follicle unblocking, anti-inflammatory and antibacterial effects, barrier repair, and hair care. It also exhibits excellent performance in terms of enzyme activity stability, gentleness and non-irritation, and synergistic effects. It can fundamentally solve problems such as clogged hair follicles, skin inflammation, hair loss, and dandruff in pets, meeting the skin and hair follicle care needs of pets of all ages and skin types.

[0004] To achieve the above objectives, the present invention adopts the following technical solution: An enzyme-containing anti-inflammatory composition for the health of pet skin and hair follicles, said composition being prepared from the following raw materials in parts by weight: The total weight of the enzyme preparation is 1.5-6 parts, the hair follicle nourishing ingredients are 3-8 parts, the functional auxiliary ingredients are 2-5 parts, and the remaining carrier raw materials are 100 parts. The enzyme preparation comprises 0.5-2 parts serine protease, 0.5-2 parts lipase, and 0.5-2 parts superoxide dismutase (SOD); the hair follicle nourishing ingredients comprise 0.1-0.5 parts biotin, 0.5-2 parts panthenol, 1-3 parts sophora flavescens extract, 0.5-1.5 parts ginger extract, 0.5-1.5 parts fish oil, 0.5-1 part egg yolk powder, and 0.3-0.8 parts turmeric extract; the functional auxiliary ingredients comprise 0.5-1.5 parts ceramide, 1-2.5 parts lanolin, and 0.2-0.5 parts phenoxyethanol; the carrier raw materials comprise 60-85 parts deionized water, 5-12 parts glycerin, 3-8 parts propylene glycol, and 0.1-0.4 parts xanthan gum.

[0005] Optionally, the lipase is a specific lipase derived from Candida albicans, and the serine protease is a mild protease derived from Bacillus subtilis. Both are modified with polyethylene glycol to modify the surface active groups of the enzyme molecules, and the mass ratio of the two after modification is 1:(0.9-1.1).

[0006] Optionally, the mass ratio of serine protease, lipase, and superoxide dismutase in the enzyme preparation is 1:(0.9-1.1):(0.9-1.1); the mass ratio of Sophora flavescens extract to turmeric extract in the hair follicle care ingredients is 3:(0.9-1.1), the mass ratio of biotin to Sophora flavescens extract is 1:(4.5-5.5), and the mass ratio of fish oil to ginger extract is 1:(0.9-1.1).

[0007] Optionally, the mass ratio of ceramide to panthenol in the functional auxiliary ingredients is 1:(1.4-1.6), and the mass ratio of lanolin to ceramide is 2:(0.9-1.1).

[0008] Optionally, the matrine content of the Sophora flavescens extract is 5%-12%, the curcumin content of the turmeric extract is 8%-15%, and the total content of eicosapentaenoic acid and docosahexaenoic acid in the fish oil is 30%-50%.

[0009] Optionally, the composition is in the form of a topical ointment, spray, or shower gel, used to prepare pet skin care products that improve pet hair follicle blockage, skin inflammation, hair loss, and dandruff problems.

[0010] Optionally, the method for preparing the enzyme-containing anti-inflammatory composition for pet skin and hair follicle health is characterized by the following specific preparation steps: S1. Modified serine protease, lipase, and superoxide dismutase are mixed in a certain proportion to form an enzyme mixture. The enzyme mixture is then mixed with chitosan solution at a volume ratio of 1:(2.8-3.2). The mixture is stirred at 22-28℃ and 380-420 rpm for 25-35 min. Sodium alginate solution is then slowly added dropwise, and stirring is continued for 55-65 min. The mixture is then freeze-dried at -55℃ to -45℃ for 7-9 h to obtain enzyme microcapsules. S2. Ceramide and lanolin are mixed in a certain proportion and ultrasonically emulsified at (280-320) W for 15-25 min to form a composite barrier repair agent with nano-sized liposomes. S3. Mix deionized water, glycerol, propylene glycol and xanthan gum in proportion, and stir at 50-55℃ and 300-400 rpm for 25-35 min to obtain the carrier matrix. S4. When the carrier matrix is ​​cooled to 28-32℃, add enzyme microcapsules, sophora flavescens extract, ginger extract, fish oil, egg yolk powder, and turmeric extract, and gently stir at 300-400 rpm for 55-65 min. S5. Mix the composite barrier repair agent and panthenol in proportion and add them to the system. Continue stirring for 20-30 min. Finally, add phenoxyethanol and stir at low speed of 100-200 rpm for 15-25 min to obtain the composition.

[0011] Optionally, the sodium alginate solution in step S1 has a mass concentration of 1%-3% and a dropping rate of 1-3 mL / min.

[0012] The beneficial effects of this invention are: The enzyme-containing anti-inflammatory composition prepared by this invention has excellent hair follicle unblocking effect, degrading more than 85% of sebum and keratin debris in hair follicles within 30 minutes without damaging hair follicle stem cells; its inhibition rate against pathogenic bacteria such as Staphylococcus aureus and Malassezia is over 80%, with significant anti-inflammatory and antibacterial effects; the synergistic effect of the compound components in the composition increases the hair follicle regeneration rate of pets by more than 45%, while shortening the repair time of damaged skin and hair follicle barrier by more than 60%, effectively improving hair loss and dandruff problems; through microencapsulation and low-temperature preparation process, the enzyme activity retention rate is over 95%, and the activity loss during the shelf life is no more than 8%; moreover, the composition is mild and non-irritating, suitable for pets of all ages and skin types, and can protect the health of pet skin and hair follicles from multiple dimensions such as unblocking, anti-inflammation, repair, and maintenance, with comprehensive performance far exceeding traditional care products. Attached Figure Description

[0013] The accompanying drawings are provided to further illustrate the invention and form part of the specification. They are used together with the embodiments of the invention to explain the invention and do not constitute a limitation thereof.

[0014] Figure 1 This is a bar chart comparing the oil decomposition rates of different samples in this invention. Figure 2 This is a bar chart comparing the enzyme activity retention rates of different samples under normal temperature and accelerated aging conditions according to the present invention. Detailed Implementation

[0015] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0016] Example 1: This Example 1 describes an enzyme-containing anti-inflammatory composition for pet skin and hair follicle health, prepared from the following raw materials in parts by weight: The product contains 3 parts of enzyme preparation (1 part of serine protease, 1 part of lipase, and 1 part of SOD), 5.9 parts of hair follicle nourishing ingredients (0.2 parts of biotin, 1 part of panthenol, 1.5 parts of sophora flavescens extract, 1 part of ginger extract, 1 part of fish oil, 0.7 parts of egg yolk powder, and 0.5 parts of turmeric extract), 3.5 parts of functional auxiliary ingredients (1 part of ceramide, 2 parts of lanolin, and 0.5 parts of phenoxyethanol), and 87.6 parts of carrier raw materials (75.3 parts of deionized water, 8 parts of glycerin, 4 parts of propylene glycol, and 0.3 parts of xanthan gum). This embodiment describes a method for preparing an enzyme-containing anti-inflammatory composition for pet skin and hair follicle health. The specific preparation steps are as follows: S1. Take serine protease from Bacillus subtilis and lipase from Candida albicans, and add them to a 5% PEG solution at a material-to-liquid ratio of 1:10 (enzyme powder: PEG solution, g / mL). Stir the reaction at 25℃ and 150 rpm for 2 hours to complete the site-specific modification of the active groups on the enzyme surface. After the reaction, freeze-dry the enzyme at -50℃ for 4 hours to obtain PEG-modified enzyme powder for later use. S2. The modified serine protease, lipase and SOD were mixed in a ratio of 1:1:1 to form an enzyme mixture. The enzyme mixture was then mixed with chitosan solution in a volume ratio of 1:3. The mixture was stirred at 25℃ and 400 rpm for 30 min. A 2% sodium alginate solution was added dropwise at a rate of 2 mL / min. The mixture was stirred for another 60 min and then freeze-dried at -50℃ for 8 h to obtain enzyme microcapsules. S3. Mix ceramide and lanolin at a ratio of 1:2, and emulsify by ultrasonication at 300 W for 20 min to form a composite barrier repair agent with nano-sized liposomes. S4. Mix deionized water, glycerol, propylene glycol and xanthan gum in a ratio of 75.3:8:4:0.3 and stir at 52℃ and 350 rpm for 30 min to obtain the carrier matrix. S5. When the carrier matrix is ​​cooled to 30°C, add enzyme microcapsules, sophora flavescens extract, ginger extract, fish oil, egg yolk powder, and turmeric extract, and gently stir at 350 rpm for 60 min. S6. After mixing the composite barrier repair agent with panthenol, add it to the system and continue stirring for 25 min. Finally, add phenoxyethanol and stir at low speed of 150 rpm for 20 min to obtain the composition.

[0017] Example 2: This Example 2 presents an enzyme-containing anti-inflammatory composition for pet skin and hair follicle health, prepared from the following raw materials in parts by weight: The product contains 3 parts of enzyme preparation (1 part of serine protease, 0.9 parts of lipase, and 1.1 parts of SOD), 5.9 parts of hair follicle nourishing ingredients (0.2 parts of biotin, 1 part of panthenol, 1.5 parts of sophora flavescens extract, 1 part of ginger extract, 1 part of fish oil, 0.7 parts of egg yolk powder, and 0.5 parts of turmeric extract), 3.5 parts of functional auxiliary ingredients (1 part of ceramide, 2 parts of lanolin, and 0.5 parts of phenoxyethanol), and 87.6 parts of carrier raw materials (75.3 parts of deionized water, 8 parts of glycerin, 4 parts of propylene glycol, and 0.3 parts of xanthan gum). In this embodiment, the preparation method of an enzyme-containing anti-inflammatory composition for pet skin and hair follicle health is the same as in Example 1, except that the ratio of enzyme components is changed to 1:0.9:1.1.

[0018] Example 3: This Example 3 presents an enzyme-containing anti-inflammatory composition for pet skin and hair follicle health, prepared from the following raw materials in parts by weight: The product contains 3 parts of enzyme preparation (1 part of serine protease, 1 part of lipase, and 1 part of SOD), 6.5 parts of hair follicle nourishing ingredients (0.2 parts of biotin, 1.6 parts of panthenol, 1.5 parts of sophora flavescens extract, 1 part of ginger extract, 1 part of fish oil, 0.7 parts of egg yolk powder, and 0.5 parts of turmeric extract), 3.5 parts of functional auxiliary ingredients (1 part of ceramide, 2 parts of lanolin, and 0.5 parts of phenoxyethanol), and 87 parts of carrier raw materials (74.7 parts of deionized water, 8 parts of glycerin, 4 parts of propylene glycol, and 0.3 parts of xanthan gum). In this embodiment, the preparation method of an enzyme-containing anti-inflammatory composition for pet skin and hair follicle health is the same as in Example 1, except that the panthenol in the hair follicle care ingredient is changed to 1.6 parts.

[0019] Comparative Example 1: The composition of Comparative Example 1 was prepared from the following raw materials in parts by weight: The product contains 3 parts of enzyme preparation (1 part of serine protease, 1 part of lipase, and 1 part of SOD), 5.9 parts of hair follicle nourishing ingredients (0.2 parts of biotin, 1 part of panthenol, 1.5 parts of sophora flavescens extract, 1 part of ginger extract, 1 part of fish oil, 0.7 parts of egg yolk powder, and 0.5 parts of turmeric extract), 3.5 parts of functional auxiliary ingredients (1 part of ceramide, 2 parts of lanolin, and 0.5 parts of phenoxyethanol), and 87.6 parts of carrier raw materials (75.3 parts of deionized water, 8 parts of glycerin, 4 parts of propylene glycol, and 0.3 parts of xanthan gum). The preparation method of the composition in this comparative example is the same as that in Example 1, except that the PEG modification step is omitted.

[0020] Comparative Example 2: The composition of Comparative Example 2 was prepared from the following parts by weight of raw materials: The product contains 3 parts of enzyme preparation (1 part of serine protease, 1 part of lipase, and 1 part of SOD), 5.9 parts of hair follicle nourishing ingredients (0.2 parts of biotin, 1 part of panthenol, 1.5 parts of sophora flavescens extract, 1 part of ginger extract, 1 part of fish oil, 0.7 parts of egg yolk powder, and 0.5 parts of turmeric extract), 3.5 parts of functional auxiliary ingredients (1 part of ceramide, 2 parts of lanolin, and 0.5 parts of phenoxyethanol), and 87.6 parts of carrier raw materials (75.3 parts of deionized water, 8 parts of glycerin, 4 parts of propylene glycol, and 0.3 parts of xanthan gum). The preparation method of the composition in this comparative example is the same as that in Example 1, except that the enzyme microcapsule encapsulation step is omitted. The carrier matrix is ​​cooled to 30°C, and PEG-modified serine protease, lipase, and SOD (unencapsulated enzyme mixture) are added directly. Then, hair follicle nourishing ingredients are added, and the mixture is stirred at 350 rpm for 60 min.

[0021] Comparative Example 3: The composition of Comparative Example 3 was prepared from the following raw materials in parts by weight: The product contains 3 parts of enzyme preparation (1 part of serine protease, 1 part of lipase, and 1 part of SOD), 5.9 parts of hair follicle nourishing ingredients (0.2 parts of biotin, 1 part of panthenol, 1.5 parts of sophora flavescens extract, 1 part of ginger extract, 1 part of fish oil, 0.7 parts of egg yolk powder, and 0.5 parts of turmeric extract), 3.5 parts of functional auxiliary ingredients (1 part of ceramide, 2 parts of lanolin, and 0.5 parts of phenoxyethanol), and 87.6 parts of carrier raw materials (75.3 parts of deionized water, 8 parts of glycerin, 4 parts of propylene glycol, and 0.3 parts of xanthan gum). The preparation method of the composition in this comparative example is the same as that in Example 1, except that the ultrasonic emulsification process is omitted in step S3 and replaced with stirring at 300 rpm for 20 min.

[0022] Performance testing 1. Test on the effectiveness of unclogging hair follicles First, artificial simulated hair follicle blockage samples were prepared. Lanolin and keratin were mixed evenly at a mass ratio of 3:1 and filled into a simulated hair follicle carrier (a porous fiber membrane with a pore size of 50-100 μm) to make samples containing 0.5 g of blockage material per piece. Then, 2 g of each of the compositions of Examples 1-3 and Comparative Examples 1-3 were taken and evenly applied to the surface of the corresponding samples. The samples were incubated in a constant temperature environment of 32℃ (simulating the skin temperature of pets) for 2 h, and gently shaken every 30 min to simulate the skin metabolism state. After incubation, the samples were rinsed 3 times with physiological saline, the residual blockage material was collected and freeze-dried to constant weight, and the oil decomposition rate was calculated (oil decomposition rate = (initial blockage material mass - residual blockage material mass) / initial blockage material mass × 100%).

[0023] Table 1. Test data on the effect of different samples on unblocking hair follicles. The oil decomposition rates of the compositions in Examples 1-3 of this invention are all higher than 87%, demonstrating excellent pore-clearing effects. Comparative Example 1, lacking PEG modification, and Comparative Example 3, lacking ultrasonic emulsification, had decomposition rates reduced to 56.8% and 69.0%, respectively. Comparative Example 2, lacking microencapsulation, had a slightly lower decomposition rate than the Example groups. These results demonstrate that PEG modification, microencapsulation, and ultrasonic emulsification are key technical features for improving hair follicle pore clearing effects.

[0024] 2. Enzyme activity retention rate test Take 10 g of each of the compositions from Examples 1-3 and Comparative Examples 1-3, and divide them into two groups. One group is stored at room temperature (25°C, 60% relative humidity), and the other group is stored in an accelerated aging environment (40°C, 75% relative humidity). Each group has three parallel samples. Samples are taken on storage days 0, 30, 60, and 90. The activity of serine protease is determined by the Folin-Ciocalteu method (using tyrosine as a standard, the absorbance value is measured at a wavelength of 660 nm to calculate the activity), and the activity of lipase is determined by titration (using olive oil as a substrate, the amount of fatty acids released is titrated to calculate the activity). Based on the initial enzyme activity (day 0) and the enzyme activity at each time point, the enzyme activity retention rate is calculated (enzyme activity retention rate = enzyme activity after storage / initial enzyme activity × 100%). The differences in activity decay between the groups under different storage conditions are compared.

[0025] Table 2. Data on enzyme activity retention rate of different samples under room temperature storage Table 3 Enzyme activity retention rate under accelerated aging storage conditions for different samples After 90 days of storage at room temperature, the enzyme activity retention rates of Examples 1-3 were all above 80%, and remained above 70% even under accelerated aging conditions. In contrast, Comparative Example 2, lacking microencapsulation, experienced a sharp decline in enzyme activity, with a retention rate of only 35.2% after 90 days at room temperature and only 15.7% after accelerated aging. This demonstrates that microencapsulation is the core process for ensuring long-term stable enzyme activity, PEG modification can slightly improve stability, and ultrasonic emulsification has no significant impact.

[0026] 3. Anti-inflammatory activity test An in vitro pet skin fibroblast inflammation model was used (healthy canine skin fibroblasts were selected), and the cells were seeded in 96-well plates (5×10⁶ cells / wells). 4 Cells were cultured for 24 h until adherence was achieved. The original culture medium was discarded, and culture medium containing 1 μg / mL lipopolysaccharide (LPS) was added to induce an inflammatory response. After incubation for 6 h, the experimental groups were added with culture medium containing the compositions of Examples 1-3 and Comparative Examples 1-3 (final concentration 5%), the blank control group was added with basal culture medium without the compositions, and the positive control group was added with culture medium containing commercially available pet anti-inflammatory care products (final concentration 5%). Three parallel wells were set up for each group. After culturing for another 24 h, the supernatant of each well was collected, and the concentrations of inflammatory factors IL-6 and histamine were measured by enzyme-linked immunosorbent assay (ELISA). The inflammation inhibition rate was calculated (inflammation inhibition rate = (concentration of inflammatory factors in the model group - concentration of inflammatory factors in the experimental group) / concentration of inflammatory factors in the model group × 100%).

[0027] Table 4. Data on anti-inflammatory activity of different samples The compositions of Examples 1-3 showed inhibition rates of over 80% against IL-6 and histamine, significantly superior to the commercially available positive control group. The inhibition rates of Comparative Examples 1-3 were lower than those of the Example groups, with Comparative Example 3 exhibiting the worst anti-inflammatory effect due to the absence of ultrasonic emulsification. This indicates that the compositions of the present invention possess excellent anti-inflammatory activity, and that ultrasonic emulsification and the ratio of Sophora flavescens to Curcuma longa can enhance the anti-inflammatory effect.

[0028] 4. Skin irritation test Eight healthy adult Beagles (weighing 8-10 kg, half male and half female) were selected. After 7 days of acclimatization, seven intact skin test areas (2 cm × 2 cm) were marked on both sides of the spine on the back of each dog (avoiding broken or inflamed areas). These areas were labeled as Example 1-3, Comparative Examples 1-3, and the blank control group. Before the experiment, the skin of the test areas was cleaned with warm water and allowed to air dry. 0.5 g of the corresponding composition was applied evenly to each Example group, while an equal amount of physiological saline was applied to the blank control group. The areas were covered and fixed with sterile gauze and kept for 4 hours. After 4 hours, the residual composition was removed, and the areas were gently washed with warm water. The irritation reactions such as erythema, edema, and desquamation were observed and recorded at 24 h, 48 h, and 72 h after application. The skin irritation reaction scoring criteria (erythema 0-4 points, edema 0-4 points) of each area were used to score the reactions. The average irritation score for each group was calculated. An average score ≤ 0.5 points was considered non-irritating, and 0.5-2.0 points was considered mildly irritating. A score of 2.0 indicates moderate to severe irritation; the skin safety of the composition will be assessed comprehensively.

[0029] Table 5. Data on skin irritation of different samples The average total irritation score of the compositions in Examples 1-3 and Comparative Examples 1-3 was ≤0.17, meeting the criteria for non-irritation. Only Comparative Example 2 showed a slight edema score due to the lack of enzyme encapsulation, but no substantial irritation was observed. These results demonstrate that the compositions of the present invention are gentle and non-irritating to pet skin, and the microencapsulation process can further optimize skin compatibility.

[0030] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An enzyme-containing anti-inflammatory composition for pet skin and hair follicle health, characterized in that, The composition is prepared from the following raw materials in parts by weight: The total weight of the enzyme preparation is 1.5-6 parts, the hair follicle nourishing ingredients are 3-8 parts, the functional auxiliary ingredients are 2-5 parts, and the remaining carrier raw materials are 100 parts. The enzyme preparation comprises 0.5-2 parts serine protease, 0.5-2 parts lipase, and 0.5-2 parts superoxide dismutase; the hair follicle nourishing ingredients comprise 0.1-0.5 parts biotin, 0.5-2 parts panthenol, 1-3 parts sophora flavescens extract, 0.5-1.5 parts ginger extract, 0.5-1.5 parts fish oil, 0.5-1 part egg yolk powder, and 0.3-0.8 parts turmeric extract; the functional auxiliary ingredients comprise 0.5-1.5 parts ceramide, 1-2.5 parts lanolin, and 0.2-0.5 parts phenoxyethanol; the carrier raw materials comprise 60-85 parts deionized water, 5-12 parts glycerin, 3-8 parts propylene glycol, and 0.1-0.4 parts xanthan gum.

2. The enzyme-containing anti-inflammatory composition for pet skin and hair follicle health according to claim 1, characterized in that, The lipase is a specific lipase derived from Candida albicans, and the serine protease is a mild protease derived from Bacillus subtilis. Both are modified with polyethylene glycol to modify the surface active groups of the enzyme molecules, and the mass ratio of the two after modification is 1:(0.9-1.1).

3. The enzyme-containing anti-inflammatory composition for pet skin and hair follicle health according to claim 1, characterized in that, The mass ratio of serine protease, lipase, and superoxide dismutase in the enzyme preparation is 1:(0.9-1.1):(0.9-1.1); the mass ratio of Sophora flavescens extract to turmeric extract in the hair follicle care ingredients is 3:(0.9-1.1), the mass ratio of biotin to Sophora flavescens extract is 1:(4.5-5.5), and the mass ratio of fish oil to ginger extract is 1:(0.9-1.1).

4. The enzyme-containing anti-inflammatory composition for pet skin and hair follicle health according to claim 1, characterized in that, The functional auxiliary ingredients have a ceramide to panthenol mass ratio of 1:(1.4-1.6) and a lanolin to ceramide mass ratio of 2:(0.9-1.1).

5. The enzyme-containing anti-inflammatory composition for pet skin and hair follicle health according to claim 1, characterized in that, The matrine content of the Sophora flavescens extract is 5%-12%, the curcumin content of the turmeric extract is 8%-15%, and the total content of eicosapentaenoic acid and docosahexaenoic acid in the fish oil is 30%-50%.

6. The enzyme-containing anti-inflammatory composition for pet skin and hair follicle health according to claim 1, characterized in that, The composition is in the form of a topical ointment, spray, or shower gel, and is used to prepare pet skin care products that improve pet hair follicle blockage, skin inflammation, hair loss, and dandruff problems.

7. A method for preparing an enzyme-containing anti-inflammatory composition for pet skin and hair follicle health according to any one of claims 1-6, characterized in that, The specific preparation steps are as follows: S1. Modified serine protease, lipase, and superoxide dismutase are mixed in a certain proportion to form an enzyme mixture. The enzyme mixture is then mixed with chitosan solution at a volume ratio of 1:(2.8-3.2). The mixture is stirred at 22-28℃ and 380-420 rpm for 25-35 min. Sodium alginate solution is then slowly added dropwise, and stirring is continued for 55-65 min. The mixture is then freeze-dried at -55℃ to -45℃ for 7-9 h to obtain enzyme microcapsules. S2. Ceramide and lanolin are mixed in a certain proportion and ultrasonically emulsified at (280-320) W for 15-25 min to form a composite barrier repair agent with nano-sized liposomes. S3. Mix deionized water, glycerol, propylene glycol and xanthan gum in proportion, and stir at 50-55℃ and 300-400 rpm for 25-35 min to obtain the carrier matrix. S4. When the carrier matrix is ​​cooled to 28-32℃, add enzyme microcapsules, sophora flavescens extract, ginger extract, fish oil, egg yolk powder, and turmeric extract, and gently stir at 300-400 rpm for 55-65 min. S5. Mix the composite barrier repair agent and panthenol in proportion and add them to the system. Continue stirring for 20-30 min. Finally, add phenoxyethanol and stir at low speed of 100-200 rpm for 15-25 min to obtain the composition.

8. A method for preparing an enzyme-containing anti-inflammatory composition for pet skin and hair follicle health according to claim 5, characterized in that, In step S1, the sodium alginate solution has a mass concentration of 1%-3% and a dropping rate of 1-3 mL / min.

Images