A short-hair grain fungus and application

Abstract

The application discloses a short woolly grain hair fungus (B. subglobosus) Lachnum brevipilosum ) and application, and belongs to the field of fungal biotechnology; the strain is separated from tender branches of Vaccinium dunalianum, can be used as a microbial source of natural woody flavoring, and especially, (-)-isoralbene is found in microbial metabolites for the first time, so that a blank of synthesis of woody fragrance components of microbial sources is filled; the application method comprises the following steps of strain activation, solid fermentation culture and dynamic collection of headspace volatiles, and the fermentation product is a complex fragrance containing components such as alcohols and olefins, and has rich hierarchical woody-soil-scent-spicy complex fragrance notes; the application uses a microbial fermentation mode to produce natural woody flavoring, can overcome problems of long growth cycle and unstable supply of plant raw materials, and has the advantages of controllable process, no limitation by seasons and regions, and strong sustainability.

Description

Technical Field

[0001] This invention belongs to the field of fungal biotechnology, specifically a short-haired granular disc fungus and its application. Background Technology

[0002] Aroma compounds are a class of volatile molecules with characteristic odors, mainly including terpenes, esters, aldehydes, alcohols, etc., which are widely used in high-end perfumes, cosmetics, food flavorings and aromatherapy. Among them, woody aromas, due to their unique warmth, calmness, smokiness and earthy base, occupy an irreplaceable core position in creating luxurious, long-lasting and natural fragrance systems.

[0003] At present, commercial woody fragrances are mainly derived from plant extraction. However, this source faces the following key bottlenecks: (1) Resource scarcity and ecological pressure: Traditional woody fragrance components (such as essential oils derived from precious woods such as sandalwood and agarwood) rely on plant resources with growth cycles of decades or even hundreds of years; long-term over-logging has led to the depletion of these species resources and caused serious ecological problems; (2) Unstable supply and high cost: The yield and quality of plant sources are constrained by multiple factors such as planting area, growing season, climate conditions and pests and diseases, resulting in poor supply stability and market prices that remain high; (3) Complex extraction process and component fluctuation: The process of extracting essential oils from plant tissues is complex and the yield is low. Moreover, the composition and proportion of aroma components of the final product are prone to significant differences due to differences in place of origin, year and extraction process, making it difficult to meet the standardization requirements of industrial production.

[0004] To address these challenges, some studies have proposed chemical synthesis methods that can partially mimic woody aromas. However, chemically synthesized products are simple, lacking in aroma complexity and vibrancy, and are not as complex as natural aromas. Furthermore, chemically synthesized fragrances do not possess the "natural" attribute and cannot meet the current market's urgent demand for "natural" and "green and sustainable" products.

[0005] Microbial fermentation and biotransformation technology, as a new generation of "cell factories," offers a promising solution for the sustainable production of natural fragrances. Compared with traditional plant and animal extraction methods, this method has significant advantages such as shorter cycle, no geographical or seasonal limitations, and controllable process. However, current research on aroma-producing microorganisms is mostly focused on traditional fermentation strains such as Saccharomyces cerevisiae and Lactic acid bacteria, whose aromas are mainly fruity and ester-based. There is little exploration and development of non-traditional microbial resources such as filamentous fungi that can synthesize complex lignin-terpene compounds, which have enormous potential for exploration.

[0006] Therefore, the targeted discovery and screening of new strains capable of efficiently synthesizing woody volatile organic compounds (VOCs) from the vast microbial resources has become a key technological breakthrough for overcoming the raw material bottleneck in this field and achieving a sustainable supply of natural woody fragrances. This will not only provide the fragrance industry with stable and natural new raw materials, but also play an important role in protecting endangered plant resources and promoting green biomanufacturing. Summary of the Invention

[0007] To solve or partially solve the problems existing in the prior art, one of the objectives of this invention is to provide a *Shorthair granular discus* (… Lachnum brevipilosum The short-haired granular discus ( Lachnum brevipilosum It was deposited on December 26, 2025, at the China General Microbiological Culture Collection Center, located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences. The proposed classification name is *Cladosporium breviculatum*. Lachnum brevipilosum The accession number is CGMCC No. 42485.

[0008] The second objective of this invention is to provide a *Short-haired Cladosporium* (Short-haired Cladosporium) Lachnum brevipilosum Application of woody fragrance in production.

[0009] The third objective of this invention is to provide a *Shorthair-haired Cladosporium* (Shorthair-haired Cladosporium) Lachnum brevipilosum The method for producing woody fragrance is described in the following steps: [The text then abruptly shifts to a description of a fungus called *Pterocarya shorthair* (likely a type of fungus), which is not directly related to the preceding sentence about woody fragrance.] Lachnum brevipilosum The culture was carried out on an inoculation medium, and volatile metabolites were collected, including woody aromatic compounds.

[0010] Preferably, the volatile metabolites of the present invention include one or more of ethanol, isoamyl alcohol, styrene, γ-ylang-olene, (-)-iso-tertillerene, or dehydro-acorene, wherein the woody aroma includes one or more of γ-ylang-olene, (-)-iso-tertillerene, or dehydro-acorene.

[0011] Preferably, the culture medium described in this invention is PSA medium (potato sucrose agar medium).

[0012] Preferably, the culture temperature described in this invention is 26-28℃.

[0013] Beneficial effects of this invention:

[0014] (1) Innovatively obtained a new natural source of woody fragrance: This invention is the first to discover a strain of *Cladosporium brevicornum* capable of producing natural woody fragrance compounds. Lachnum brevipilosumThe volatile metabolites produced mainly include one or more of ethanol, isoamyl alcohol, styrene, γ-ylang-olene, (-)-iso-tertene, and dehydro-acorene, among which the woody aroma includes one or more of γ-ylang-olene, (-)-iso-tertene, and dehydro-acorene; in particular, the production of (-)-iso-tertene was reported for the first time in microbial volatiles, filling the gap in the research on the synthesis pathway of this key aroma component in microbial woody fragrances.

[0015] (2) The product has “natural” attributes and rich aroma layers: the volatile organic compounds obtained directly through microbial fermentation meet the market demand for “natural” fragrances; at the same time, the method produces not a single compound, but a complex mixture containing multiple components such as alcohols and alkenes, which together form a rich, harmonious and natural woody-soily-spicy complex aroma, which is superior to the single aroma of chemically synthesized fragrances.

[0016] (3) It provides a sustainable and stable supply solution: Through microbial fermentation, the present invention can achieve production that is not limited by season or region and the process is controllable; it overcomes the problems of unstable supply and high price caused by the long growth cycle, scarcity of resources, and great influence of environmental factors of traditional plant-derived woody flavorings, and provides a stable and sustainable new way for the production of natural woody flavorings.

[0017] (4) The analytical methods are accurate and reliable, providing a guarantee for product identification and quality control: a highly sensitive and accurate detection and identification method for such microbial volatiles has been established; it can clearly distinguish background interference, accurately identify trace amounts of specific volatile components, and effectively identify compounds (such as (-)-iso-tertene). Attached Figure Description

[0018] Figure 1 The *Shorthair-haired Cladosporium* in Example 1 of this invention (… Lachnum brevipilosum The change in diameter over time during growth on PDA medium.

[0019] Figure 2 The *Cladosporium brevis* strain on PDA medium in Example 1 of this invention (… Lachnum brevipilosum The growth status of ); among which Figure 2 a is *Cladosporium breviculatum* ( Lachnum brevipilosum Growth status on PDA medium; Figure 2 b is *Cladosporium breviculatum* ( Lachnum brevipilosum Morphological features of hyphae and other parts under a 40x microscope; Figure 2 c represents *Cladosporium breviculatum* (a type of bacteria). Lachnum brevipilosum Morphological characteristics of spores under a 40x microscope.

[0020] Figure 3The present invention is based on the short-haired granular discus ( Lachnum brevipilosum Phylogenetic tree diagram of ).

[0021] Figure 4 The present invention is based on the short-haired granular discus ( Lachnum brevipilosum Schematic diagram of the chemical structures of the six VOCs produced.

[0022] Figure 5 The present invention is based on the short-haired granular discus ( Lachnum brevipilosum Total ion chromatograms of the six VOCs generated by GC-MS analysis.

[0023] Figure 6 This is the total ion chromatogram of the blank PSA culture medium in Comparative Example 1 of this invention, obtained by GC-MS analysis.

[0024] Figure 7 The *Shorthair-haired Cladosporium* in Example 3 of this invention (… Lachnum brevipilosum The graph shows the relative abundance changes of the six VOCs produced over time. Figure 7 In this context, 'a' represents the volatile compound ethanol. Figure 7 In this context, b represents the volatile compound isoamyl alcohol. Figure 7 In this context, c represents styrene, a volatile compound. Figure 7 In the middle, d represents the volatile compound γ-ylangolene. Figure 7 In this context, e represents the volatile compound (-)-iso-tertene. Figure 7 f represents the volatile compound dehydroacorene.

[0025] Figure 8 The *Shorthair-haired Cladosporium* in Example 6 of this invention (… Lachnum brevipilosum The correlation between the six VOCs produced and the growth diameter of fungi. Detailed Implementation

[0026] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. However, the scope of protection of the present invention is not limited thereto. Unless otherwise specified, all reagents used in the present invention are commercially available analytical grade reagents.

[0027] Example 1

[0028] The short-haired granular disc fungus described in this invention ( Lachnum brevipilosum The separation, identification, and preservation of ( ) specifically include the following steps:

[0029] (1) Prepare and sterilize PDA medium and Martin's medium. The specific preparation method is as follows:

[0030] PDA medium: Take 200.0g of commercially available fresh potatoes, 20.0g of glucose, and 18.0g of microbial grade agar powder, add distilled water to a final volume of 1.0L, set the pH to natural, and autoclave at 121℃ for 25min.

[0031] Martin's medium: 10.0g glucose, 5.0g peptone, 1.0g potassium dihydrogen phosphate, 0.5g magnesium sulfate heptahydrate, 15.0g agar, add distilled water to a final volume of 1.0L, set pH to natural, autoclave at 121℃ for 25min.

[0032] The sterilized culture medium is poured into sterile petri dishes in the laminar flow hood and allowed to solidify before use. The laminar flow hood is sterilized by wiping with 75% alcohol and irradiating with ultraviolet light for 30 minutes before use.

[0033] (2) Surface disinfection and endophytic bacteria isolation of plant materials:

[0034] Tender branches of healthy camphor-leaved blueberries from Wuding County, Yunnan Province, were used as the isolation material. They were soaked in a dish soap solution for 2 minutes, rinsed under running water for 30 minutes, and then placed on sterile filter paper to absorb surface moisture. They were then transferred to a clean bench and disinfected by immersion in 75% ethanol for 30 seconds, followed by immersion in 0.1% HgCl2 solution for 3 minutes. After disinfection, they were immediately rinsed with sterile water until all residual disinfectant was removed. After absorbing moisture with sterile filter paper, the tender branches were cut into small segments of about 0.5 cm in length using sterile scissors. The segments were then placed flat on the surface of PDA culture medium, with 3-4 segments inoculated per dish. The culture dishes were sealed with sealing film and incubated upside down in a 28°C constant temperature incubator.

[0035] To verify the surface disinfection effect, a control experiment was set up: the sterile water of the last rinse material was applied to the blank PDA (Martin's medium can also be used as needed), and the blank PDA was exposed in the clean bench for 10 minutes, and then cultured together with the experimental group; the disinfection was considered to be thorough only when no colonies grew in the control group, and the colonies that grew from the experimental group were considered to be endophytic fungi.

[0036] (3) Strain purification:

[0037] After mycelia grow from the edges of the tender branch cuttings, under aseptic conditions, based on differences in colony morphology (color, texture, edge characteristics, etc.), mycelia from the edge of a single colony are picked with an inoculation needle and transferred to the center of a fresh PDA plate. The plate is then incubated upside down at 28°C. The process of purifying the strain is repeated until a pure culture with uniform colony morphology and no contaminants is obtained under a microscope. The purified target strain is numbered VDBF-38 and used for subsequent research.

[0038] (4) Macroscopic and microscopic morphological observations were performed on the purified strain VDBF-38.

[0039] Colony morphology observation: The purified strain VDBF-38 was inoculated into the center of a PDA plate using the spot inoculation method and incubated upside down at 28°C. Colony characteristics were observed and recorded periodically, including: colony diameter, color, raised shape, surface texture (fluffy, cottony, etc.), and edge features. The observation results are as follows: Figure 1 As shown, it can be seen that *Cladosporium breviculatum* (a type of bacteria) was cultured on days 4, 11, and 19. Lachnum brevipilosum The colony diameters were 1.1 cm, 1.5 cm, and 1.9 cm, respectively; Figure 2 As shown in Figure a, the colony is white, with an irregularly raised center, a velvety surface, and wavy edges.

[0040] Microscopic morphological observation: Specimens were prepared using the slide culture method. A sterile coverslip was inserted obliquely at approximately 45° into PDA medium near the inoculation site and incubated at 28°C until hyphae grew along the coverslip. The coverslip was then removed and placed, with the mycelial side down, on a slide containing lactophenol cotton blue staining solution, and gently pressed down. The morphology of hyphae and conidia was observed and recorded under an optical microscope (40x magnification). The results are as follows: Figure 2 As shown in Figure b, its reproductive structure is an ascocarp; and it consists of... Figure 2 As can be seen from c, the spores produced by this fungus are ascospores, which are fusiform in shape and have a smooth surface.

[0041] (5) Molecular biological identification of the strain:

[0042] Fresh mycelium was scraped off, and total DNA of strain VDBF-38 was extracted using a commercially available fungal genomic DNA extraction kit. The quality and concentration of total DNA of VDBF-38 were detected by 1.0% agarose gel electrophoresis. Using the extracted DNA as a template, PCR amplification was performed using universal primers ITS1 and ITS4 for the intratranscribed spacer region (ITS) of fungal ribosomal DNA.

[0043] ITS universal primers:

[0044] ITS1: 5'-AGAAGTCGTAACAAGGTTTCCGTAGG-3' (SEQ ID NO: 1).

[0045] ITS4: 5'-TCCTCCGCTTATTGATATGC-3' (SEQ ID NO: 2).

[0046] PCR reaction system (25µL): 12.5µL of 2×Taq PCR Master Mix, 1.0µL each of primers ITS1 and ITS4, 50ng of DNA template, and ddH2O to make up to 25µL.

[0047] PCR reaction program: 94℃ pre-denaturation for 5 min; 94℃ denaturation for 30 s, 55℃ annealing for 30 s, 72℃ extension for 45 s, for a total of 35 cycles; 72℃ final extension for 10 min.

[0048] Electrophoresis: Electrophoresis was performed using DL2000 DNA Marker, GoldView nucleic acid dye, and 1.0% agarose gel.

[0049] After testing, the sample was sent to a biotechnology company for purification and bidirectional sequencing, and the ITS sequence was obtained as shown in SEQ ID NO:3.

[0050] The ITS sequences obtained from sequencing were subjected to BLASTn homology alignment in the GenBank database of NCBI, and a developmental tree was constructed, such as... Figure 3 As shown, based on the colony morphology and molecular systematics analysis, strain VDBF-38 was identified as *Cladosporium breviculatum* (…). Lachnum brevipilosum ).

[0051] (6) Preservation of strains

[0052] The above was identified as *Trichoderma shorthair* ( Lachnum brevipilosum The purified culture of strain VDBF-38 was deposited at the China General Microbiological Culture Collection Center (CGMCC) on December 26, 2025, with accession number CGMCC No. 42485.

[0053] Example 2

[0054] Short-haired granular discus ( Lachnum brevipilosum Identification and analysis of volatile organic compounds:

[0055] (1) Culture medium preparation:

[0056] PSA medium: 200g commercially available fresh potatoes, 20g sucrose, 18g microbial grade agar, distilled water to a final volume of 1L, pH at rest, sterilize at 121℃ for 25min.

[0057] (2) Activation of strains: The short-haired granular disc bacteria ( Lachnum brevipilosum Inoculate the seeds in the center of a PDA plate and incubate in the dark at 28°C for 14 days.

[0058] (3) Solid fermentation culture: Take the edge of the activated strain with a 5mm diameter punch and put it into a 500mL culture flask containing PSA solid medium as the experimental group. Incubate in the dark at 27.5℃.

[0059] To determine that the volatile metabolites were all produced by *Cladosporium breviculatum* (…), Lachnum brevipilosum This embodiment is designed to avoid inoculation with *Cladosporium brevis* (*Cladosporium brevis*). Lachnum brevipilosumThe PSA solid culture was used as a blank group and was incubated in the dark at 27.5℃.

[0060] (4) Headspace volatiles collection: from *Cladosporium breviculatum* (… Lachnum brevipilosum From day 2 to day 41 post-inoculation, every 48 hours, headspace solid-phase microextraction (HS-SPME) was used with a 50 / 30 μm DVB / CAR / PDMS extraction head to extract *Cladosporium brevis* (*Cladosporium brevis*). Lachnum brevipilosum The headspace air of the culture flask was sampled by adsorption at a temperature of 27.5℃ for 40 minutes to obtain *Cladosporium brevis* (a type of bacteria). Lachnum brevipilosum The volatile organic compounds produced.

[0061] The blank control group also used the same headspace solid-phase microextraction method to collect headspace air from the culture flasks.

[0062] (5) GC-MS analysis conditions

[0063] Immediately after sampling, the adsorbed volatile organic compounds were analyzed using gas chromatography-mass spectrometry (GC-MS).

[0064] GC conditions: HP-5MS capillary column (30m×250μm×0.25μm); carrier gas: helium, flow rate: 0.8mL / min; injection port temperature: 250℃, splitless injection; temperature program: 40℃ for 5 min, increase to 130℃ at 5℃ / min and hold for 5 min, then increase to 230℃ at 10℃ / min and hold for 2 min.

[0065] MS conditions: Electron impact (EI) ion source; 230℃; quadrupole temperature 150℃; scan range m / z 35-550.

[0066] Analysis yielded *Cladosporium brevicornum* ( ) Lachnum brevipilosum GC-MS chromatogram of volatile organic compounds.

[0067] (6) Identification of volatile substances

[0068] The obtained GC-MS chromatogram data were compared with the NIST14 standard mass spectrometry library. A match score greater than 80 (out of 100) was used as the qualitative criterion. The relative content was calculated using the peak area normalization method. The gas chromatograms of the headspace of the culture flasks collected by the experimental group are shown below. Figure 5 As shown, the gas chromatogram of the headspace gas collected from the culture flasks in the blank group is as follows. Figure 6 As shown, by comparing the chromatograms of the experimental group and the blank group, Figure 5 A chromatographic peak, unique to the medium and significantly higher in content than the background, was identified as being caused by *Cladosporium breviculatum* (…). Lachnum brevipilosumSpecific volatile organic compounds (VOCs) produced by metabolism were analyzed, and six specific VOCs were identified. Figure 7 ), and detailed information is listed in Table 1; the specific volatile organic compounds identified were searched in the microbial volatiles database (mVOC 4.0), and it was found that (-)-iso-tertene was not recorded in the microbial volatiles database.

[0069] Table 1. *Cladosporium brevicornum* ( Lachnum brevipilosum Composition of VOCs

[0070]

[0071] This embodiment, through cultivation, discovered that the *Short-haired Trichoderma* (… Lachnum brevipilosum They possess the genetic potential and metabolic pathways to synthesize these unique sesquiterpenes, which are not found in other common aroma-producing microorganisms such as yeast and lactic acid bacteria.

[0072] This embodiment utilizes dynamic headspace acquisition to monitor the dynamic generation of volatile products, helping to determine the optimal sampling time and further ensuring the stability and controllability of the target aroma compound yield, laying the foundation for large-scale and stable production. The headspace acquisition method (HS-SPME) is a non-destructive acquisition method. In addition, the HS-SPME-GC-MS coupling technology, precise operating parameters, and the use of a 50 / 30μm DVB / CAR / PDMS extraction head ensure the efficient enrichment of a broad spectrum of volatile components. Furthermore, by utilizing optimized GC-MS temperature programs and MS scanning parameters, complex volatile components were effectively separated and accurately identified using the NIST14 spectral library.

[0073] Example 3

[0074] Short-haired granular discus ( Lachnum brevipilosum Aroma characteristics analysis:

[0075] (1) Dynamic release patterns of aroma compounds

[0076] Based on the dynamic sampling and identification results of Example 2, the release timing and relative abundance changes of each key aroma compound were analyzed throughout the 41-day fermentation cycle (semi-quantitative using peak area normalization). The characteristic chromatograms of their release are shown below. Figure 5 As shown; the release timing and relative abundance changes of individual key aroma compounds are as follows:

[0077] Ethanol: exhibits discontinuous release characteristics, with the first detection occurring on day 19 of culture, and relative abundance reaching its peak on day 33. Figure 7 (a)

[0078] Isoamyl alcohol: Release window is narrow, detected only in the later stages of culture (days 35 to 39), with relative abundance reaching its peak on day 39. Figure 7 (b)

[0079] Styrene: exhibits discontinuous release characteristics, with the first detection occurring on day 11 of culture, and the relative abundance reaching its peak on day 39. Figure 7 (c)

[0080] γ-Eylanolene: Release is extremely transient, detected only on day 39 of culture. Figure 7 (d).

[0081] (-)-Iso-tertene: exhibits discontinuous release characteristics, with the first detection occurring on day 25 of culture, and the relative abundance reaching its peak on day 27. Figure 7 (e).

[0082] Dehydroaurone: Release is extremely brief ( Figure 7 (f) was detected only on day 35 of culture.

[0083] (2) Analysis of aroma composition and sensory characteristics

[0084] Based on the sensory characteristics of each compound and its relative content in the system, the analysis of *Cladosporium breviculatum* (…) was conducted. Lachnum brevipilosum The overall profile of the aroma produced:

[0085] Ethanol and isoamyl alcohol: As representatives of alcohols, low concentrations of ethanol contribute a slightly sweet fruity or ethereal aroma; while trace amounts of isoamyl alcohol blend into the overall aroma, potentially providing a complex "green" or "herbal" undertone.

[0086] Styrene: At low concentrations, its odor is characterized by a faint sweetness, a slight plasticity, and subtle floral or herbal notes, adding to the complexity of the aroma.

[0087] Terpenes (γ-ylangolene, (-)-iso-terpenesene, and dehydro-acorene): together constitute the core framework of the aroma; γ-ylangolene contributes woody, spicy, and earthy notes, with herbal and citrus undertones; (-)-iso-terpenesene (first reported as being of microbial origin) presents a complex character of woody, amber, earthy, and slightly green notes; dehydro-acorene further enriches the texture of woody and earthy notes. These terpenes together form a rich, long-lasting, and natural woody-earthy base.

[0088] (3) Correlation between aroma production and mycelial growth

[0089] Further calculations were made of the correlation coefficients between each volatile component and colony diameter, and the correlation between their relative abundance and colony growth diameter was analyzed. The results were obtained from... Figure 8It can be seen that the release of all identified VOCs is positively correlated with the colony growth diameter; among them, the production of (-)-iso-tertene has the highest correlation with the colony diameter, which indicates that its biosynthesis is closely related to the biomass accumulation of mycelium or a specific growth and development stage, and can be used as a key indicator to characterize the aroma-producing metabolic activity of this strain.

[0090] The above analysis shows that the *Short-haired Cladosporium* proposed in this invention (…) Lachnum brevipilosum (CGMCC No. 42485) During solid-state fermentation, it can systematically produce a complex aroma system composed of alcohols, aromatic hydrocarbons and various terpenoids. The aroma exhibits a dynamic release pattern and ultimately integrates multiple sensory characteristics such as fruity sweetness, greenness, floral fragrance, woody aroma, spicy aroma, amber and earthy aroma. It is rich in layers and has a natural feel. In particular, the microbial source of (-)-iso-tertene is reported for the first time, and its production is highly correlated with its growth. This proves that this strain is an excellent microbial resource for preparing novel, natural compound microbial fragrances.

[0091] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A type of short-haired granular discus ( Lachnum brevipilosum The application of *Pteris vittata* (a type of fungus) in the production of woody incense. Lachnum brevipilosum The accession number of the substance is CGMCC No. 42485; the woody fragrance is one or more of γ-ylangolene, (-)-iso-terpinene or dehydroacorene.

2. The *Short-haired Cladosporium* strain according to claim 1 (… Lachnum brevipilosum Its application in the production of woody incense is characterized by: The specific method is as follows: [The text abruptly ends here, likely due to an incomplete sentence or a missing section.] Lachnum brevipilosum The culture was carried out on an inoculation medium, and volatile metabolites were collected, including woody aromatic compounds.

3. The *Short-haired Cladosporium* strain according to claim 2 (… Lachnum brevipilosum Its application in the production of woody incense is characterized by: In addition to one or more of the woody aroma compounds selected from γ-ylangolene, (-)-iso-terpinene, or dehydroacorene, the volatile metabolites also include one or more of ethanol, isoamyl alcohol, or styrene.

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