Bacillus velezensis and application thereof

Abstract

The invention relates to bacillus velezensis and application thereof. The bacillus velezensis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36579. According to the present invention, the compound can be used for preventing and controlling at least one pathogenic bacterium selected from the group consisting of Ilyonectria, Sclerotinia sclerotiorum, Botrytis cinerea, Alternaria alternata, Rhizoctonia, Fusarium sp. And Colletotrichum gloeosporioides, and further can be used for promoting the growth and / or improving the soil fertility, and can be used for preventing and controlling at least one pathogenic bacterium selected from the group consisting of Sclerotinia sclerotiorum, Botrytis cinerea, Alternaria alternata, Rhizoctonia, Fusarium sp. And Colletotrichum gloeosporioides.

Description

Technical Field

[0001] This invention relates to the field of microorganisms, and particularly to microbial bactericides. Background Technology

[0002] Ginseng, American ginseng, and Panax notoginseng are three precious medicinal herbs belonging to the ginseng family, with high market demand and economic value. However, due to their perennial growth characteristics, diseases and soil obstacles are common during cultivation. Control of above-ground diseases mainly relies on chemical fungicides. Although frequent application of pesticides can effectively control the occurrence and spread of leaf diseases, Botrytis cinerea and Alternaria alternata, which cause leaf spot, have developed significant resistance to many fungicides, leading to increased potential risks of pesticide residue exceeding standards and greater safety hazards associated with pesticide use. Soil-borne diseases affecting the rhizomes are severe, with a short window for soil-based pesticide application and high control difficulty, significantly impacting the yield and quality of these three ginseng-related medicinal herbs.

[0003] Currently, microbial agents for controlling diseases of ginseng medicinal herbs are usually applied to the above-ground parts through foliar spraying for diseases, or through irrigation for soil-borne diseases. However, due to the dense foliage of ginseng plants during their growing season, it is difficult to control soil-borne diseases by irrigating with microbial agents. Furthermore, irrigation facilities are generally lacking in the main producing areas of Northeast China ginseng, American ginseng, and Yunnan-Guangxi Panax notoginseng. This results in a severe shortage of beneficial microbial agents that can effectively control soil-borne diseases of ginseng medicinal herbs in production.

[0004] Therefore, developing safe, efficient, and easy-to-use disease control agents is an urgent industrial challenge to be solved in the prevention and control of diseases in ginseng, a precious Chinese medicinal herb. Summary of the Invention

[0005] One of the inventions provides Bacillus belesiensis ( Bacillus velezensis The strain N8 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36579.

[0006] The second invention provides a composition comprising Bacillus belye strain N8 as described in the first invention, and an acceptable vector.

[0007] The third invention provides the Bacillus berberis strain N8 according to the first invention or the composition according to the second invention for the prevention and control of red scab (… Ilyonectria ), Sclerotinia sclerotiorum ( Sclerotinia ), Botrytis cinerea ( Botrytis ), Alternaria ( Alternaria ), Rhizoctonia solani ( Rhizoctonia Fusarium ( ) Fusarium Anthrax bacteria ( Colletotrichum Application in at least one of the pathogens in ).

[0008] In one specific embodiment, the red crust is a common red crust ( Common Iyonectria ) and / or strong red crust ( Ilyonectria robusta ).

[0009] In one specific embodiment, the *Sclerotinia sclerotiorum* is *Sclerotinia sclerotiorum* (… Sclerotinia sclerotiorum ) and / or neatly arranged sclerotia ( Sclerotinia rolfsii ).

[0010] In one specific embodiment, the botrytis cinerea is Botrytis cinerea (Glaucus spp.). Botrytis cinerea ).

[0011] In one specific embodiment, the Rhizoctonia solani is Rhizoctonia solani (… Rhizoctonia solani ).

[0012] In one specific embodiment, the Fusarium is Fusarium oxysporum (Fusarium oxysporum). Fusarium oxysporum ) and / or Fusarium solani ( Fusarium solani ).

[0013] In one specific embodiment, the anthrax bacterium is *Anthrax spp.* (Fructus *Anthrax*). Colletotrichum fruit-growing ).

[0014] In one specific embodiment, the Bacillus berberis strain N8 as described in one of the present inventions or the composition described in another of the present inventions is used to prevent and control diseases of at least one of ginseng, American ginseng and Panax notoginseng.

[0015] In one specific embodiment, the *Bacillus belyssioides* strain N8 as described in one version of the present invention or the composition described in another version of the present invention is sprayed onto the above-ground parts of the target organism. The target organism may be, for example, at least one medicinal herb selected from ginseng, American ginseng, and Panax notoginseng.

[0016] The fourth invention provides the application of the Bacillus berleis strain N8 according to the first invention or the composition according to the second invention in promoting growth and / or improving soil fertility.

[0017] In one specific embodiment, the soil fertility includes at least one of organic matter, available nitrogen, and available potassium.

[0018] Beneficial effects of the present invention: The present invention discovers the Bacillus belyssus strain screened in this invention (… Bacillus from Velez Strain N8 can be used to control red scab (a type of plant). Ilyonectria ), Sclerotinia sclerotiorum ( Sclerotinia ), Botrytis cinerea ( Botrytis ), Alternaria ( Alternaria ), Rhizoctonia solani ( Rhizoctonia Fusarium ( ) Fusarium Anthrax bacteria ( Colletotrichum It contains at least one pathogenic bacterium, and it can also promote and / or enhance soil fertility.

[0019] Bacterial strain preservation: Bacillus belye screened in this invention Bacillus velezensis Designated as N8, this strain is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36579, on November 11, 2025. The deposit address is: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. Its systematic classification is *Bacillus belyesense*. Bacillus velezensis . Attached Figure Description

[0020] Figure 1 The colony diagram of strain N8 is shown.

[0021] Figure 2 The phylogenetic tree of N8 strain 16S is shown. Figure 3 A multi-gene synergistic phylogenetic tree of strain N8 is shown.

[0022] Figure 4 The study demonstrated the in vitro control effect of strain N8 against root disease of ginseng caused by common and robust *Gnaphalium affine*.

[0023] Figure 5 The results show the effectiveness of the N8 strain in controlling ginseng leaf spot and red skin disease in the field.

[0024] Figure 6 The study demonstrated the effect of strain N8 on increasing ginseng yield.

[0025] Figure 7 The study demonstrated that strain N8 improved soil fertility. Detailed Implementation

[0026] The following preferred embodiments further illustrate the above-mentioned content of the present invention in detail, but they do not constitute a limitation on the present invention.

[0027] Unless otherwise specified, the strains and reagents used in the embodiments of this invention can be purchased commercially.

[0028] NA solid medium: 10g glucose, 10g peptone, 3g beef extract, 1g yeast extract, 18g agar, 1000mL distilled water, adjust pH to 7.0, sterilize at 121 degrees Celsius for 20 minutes.

[0029] TSA solid medium: 15g tryptone, 5g soybean peptone, 5g sodium chloride, 15g agar, 1000mL distilled water, adjust pH to 7.0, sterilize at 121 degrees Celsius for 20 minutes.

[0030] LB liquid medium: 10.0 g tryptone, 5.0 g yeast extract, 10.0 g NaCl, 1000 mL distilled water, sterilized at 121 degrees Celsius for 20 minutes.

[0031] LB solid medium: 10.0 g tryptone, 5.0 g yeast extract, 10.0 g NaCl, 15 g agar, 1000 mL distilled water, sterilized at 121 degrees Celsius for 20 minutes.

[0032] PDA medium: 200g potato, 20g glucose, 15g agar, 1000ml distilled water, natural pH, sterilized at 121°C for 30 minutes.

[0033] The common red crust used in this invention Ilyonectria common Strong red earth crust Ilyonectria robust Fusarium solani Fusarium solani For details, please see the article: Taxonomy of fungal complex causing red-skin root of Panax ginseng in China[J]; Journal of Ginseng Research; Authors: Lu XH, Zhang XM, Jiao, XLet al.; 2020, 44: 506-518.

[0034] Sclerotium Sclerotinia sclerotiorum See article: Study on pathogenicity and biological characteristics of sclerotium secretions of Ginseng Sclerotium [J]; Journal of Shenyang Agricultural University; Authors: Wang Dan, Fu Junfan, Yin Haibo, et al.; 2020, 51 (04):439-455.

[0035] Botrytis cinerea Botrytis cinereaSee article: Characterization of resistance to multiple fungicides in Botrytis cinerea populations from Asian ginseng in northern China [J]; European Journal of Plant Pathology; Authors: Lu XH, Jiao XL, Hao JJ et al.; 2016, 144(3): 467-476.

[0036] Alternaria Alternaria For details, see the article: Isolation, Identification and Control Efficacy of Biocontrol Bacteria for Ginseng Black Spot Disease [J]; Chinese Journal of Biological Control; Authors: Zhang Ning, Zhang Jingjing, Li Yashu, et al.; 2022, 38 (05): 1308-1315.

[0037] Neat small sclerotium Sclerotinia rolfsii See article: Comprehensive evaluation of the efficacy of various fungicides against white mold disease of pepper [J]; Chinese Vegetables; Authors: Wang Junying, Zhu Chunhui, Yi hope, et al.; 2023, (07):75-83.

[0038] Fusarium oxysporum Fusarium oxysporum Rhizoctonia solani Rhizoctonia solani See article: Isolation and identification of biocontrol bacteria for ginseng diseases and determination of their antibacterial activity [J]; Modern Chinese Medicine; Authors: Zhang Xiaoyun; Ding Wanlong; Wang Rong; et al.; 2018. 20(11):1387-1391.

[0039] Fruit anthracnose Colletotrichum fructicola See article: Identification of pathogen and screening of antibacterial agents for leaf spot disease of Salvia miltiorrhiza in Zhongjiang County, Sichuan Province [J]; Southwest China Journal of Agriculture; Authors: Zou Huan, Han Shuai, Fan Zhonghan, et al.; 2023, 36(12):2735-2740.

[0040] Bacillus subtilis S12 and Bacillus licheniformis ST4 were both purchased from the China Industrial Microbial Culture Collection Center.

[0041] Mycelial growth inhibition rate (%) = [(control colony radius - treated colony radius) / control colony radius] × 100%. Example 1: Isolation of strains

[0042] Rhizosphere soil from healthy ginseng plants was collected from ginseng cultivation sites in Jingyu County, Jilin Province, preserved with dry ice, and brought back to the laboratory.

[0043] Weigh 2 g of ginseng rhizosphere soil sample and add it to 18 mL of 1× phosphate buffer (pH 7.4). Place the sample on a constant temperature shaker at 200 rpm and 28°C for 30 min to ensure complete suspension. After standing for 10 min, aseptically extract the supernatant as the stock solution. Heat the stock solution in a 70°C water bath for 10 min. Then, extract 1 mL of the heated stock solution and serially dilute it with sterile water to prepare a 10-fold dilution. -3 10 -4 10 -5 Three concentration gradients of dilution were prepared. Three dilutions were spread onto NA agar plates, 100 μL per plate, with four plates for each gradient. The plates were incubated at 28°C for 3 days. Bacteria of different morphologies were picked and transferred to TSA agar for streaking purification to obtain purified isolates. Each isolate was then numbered. Example 2: Indoor antibacterial activity of the strain

[0044] This embodiment uses the isolated strain N8 as an example to describe the antibacterial activity of the strain.

[0045] Common red crust Ilyonectria common Strong red earth crust Ilyonectria robusta Sclerotium sclerotiorum Sclerotinia sclerotiorum Botrytis cinerea Botrytis cinerea Alternaria Alternaria sp., neatly arranged small sclerotium Sclerotinia rolfsii Rhizoctonia solani Rhizoctonia solani Fusarium oxysporum Fusarium oxysporum, Fruit anthracnose Colletotrichum fructicola Fusarium solani Fusarium solanum Activated on PDA medium, mycelial discs were prepared using a 5mm punch and inoculated into the center of fresh PDA plates. Simultaneously, at three of the four symmetrical cross positions (2cm from the plate edge), a straight line was drawn parallel to the tangent of the pathogenic fungal disc using N8 bacteria. The remaining position was left untouched as a blank control. *Bacillus subtilis* S12 and *Bacillus licheniformis* ST4 were used as control Bacillus species. Three replicates were set up, with one petri dish constituting one replicate. After incubation at 28°C for 3 to 10 days, the colony radius of the blank control group reached over 3.5cm. The colony diameter was measured, and the mycelial growth inhibition rate was calculated (see Table 1). The results showed that N8 had an inhibitory effect on all the above-mentioned pathogenic fungi, especially *Alternaria alternata*, *Botrytis cinerea*, *Botrytis cinerea*, and *Fusarium solani*, with significantly higher inhibition rates than *Bacillus subtilis* S12 and *Bacillus licheniformis* ST4.

[0046] Table 1

[0047] Note: Different letters after the same row data indicate that in the same row...P Significant differences were observed at the <0.05 level; the same letter indicates significance at the same level. P The difference was not significant at the <0.05 level. Example 3: Taxonomic identification of strain N8

[0048] Strains of strain N8 were cultured on LB medium at 30°C for 15 h. Colonies of strain N8 were observed to be irregularly round with irregular edges, pale yellow, opaque, and wrinkled. Figure 1 By comparing these characteristics with the morphology of strains described in the "Handbook of Systematic Identification of Common Bacteria", it is preliminarily suspected that the strain belongs to the genus Bacillus.

[0049] DNA was extracted from strain N8 using a TSINGKE extraction kit. Using strain N8 DNA as a template, the 16S rDNA sequence of strain N8 was amplified using universal primers 27F (as shown in SEQ ID No. 1) and 1492R (as shown in SEQ ID No. 2). The resulting 1401 bp fragment was sequenced by Shanghai Meiji Biotechnology Co., Ltd., and the sequence is shown in SEQ ID No. 3. The sequence was submitted to the NCBI website for homology comparison, and a phylogenetic tree was constructed using MEGA11. Figure 2 .according to Figure 2 The phylogenetic tree shows that it is closely related to strains of the genus Bacillus.

[0050] To further refine the identification of strain N8, the entire genome of strain N8 was sequenced, based on 81 core housekeeping genes from different species of Bacillus genus. alaS、cgtA、dnaG、dnaX、engA、era、ffh、fmt、frr、ftsY、 gmk、hisS、ileS、infB、infC、ksgA、lepA、leuS、nusA、nusG、pheS、pheT、prfA、recA、rplA、 rplB、rplC、rplD、rplE、rplF、rplI、rplJ、rplK、rplL、rplM、rplN、rplO、rplP、rplQ、rplR、 rplS, rplT, rplU, rplV, rplW, rplX, rpmA, rpmC, rpmI, rpoA, rpoB, rpsB, rpsC, rpsD, rpsE, rpsF, rpsG, rpsH, rpsI, rpsJ, rpsL, rpsM, rpsO, rpsP, rpsQ, rpsR, rpsS, rpsT, rsmH, ruvB, secA, secY, serS, smpB, tilS, trmD, truB, tsaD, tsf, ybeY, ychF A maximum likelihood phylogenetic tree was constructed using RAxML software, see [link / reference]. Figure 3 As shown. According to Figure 3 The results showed that strain N8 was most closely related to Bacillus belyes, thus confirming that it belonged to the Bacillus belyes species. Bacillus velezensis .

[0051] The N8 strain was deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36579 on November 11, 2025. The deposit address is: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. Its systematic classification is *Bacillus belyesense*. Bacillus velezensis . Example 4: In vitro control effect of strain N8 on ginseng red skin disease

[0052] Healthy three-year-old ginseng roots of similar growth were collected, disinfected with 75% alcohol for 2 minutes, rinsed with sterile water, and air-dried. Six treatments were set up: inoculation with only common *Scutellaria barbata* (CK + common *Scutellaria barbata*) suspension or strong *Scutellaria barbata* (CK + strong *Scutellaria barbata*) suspension, inoculation with only sterile water (blank control, CK), inoculation with only N8 inoculum (N8), inoculation with N8 inoculum followed by common *Scutellaria barbata* suspension (N8 + common *Scutellaria barbata*), and inoculation with N8 inoculum followed by strong *Scutellaria barbata* suspension (N8 + strong *Scutellaria barbata*). Each treatment was replicated three times, with six ginseng roots per replicate. The inoculation method was as follows: a sterile toothpick was used to puncture the ginseng roots at the same location to a depth of 2 mm. 100 μL of N8 aqueous suspension or sterile water (blank control) with a viable count of 0.1 billion / mL was dripped into each wound. The ginseng roots were incubated in the dark at 25 degrees Celsius. After 1 day, 100 μL of 1×10⁻⁶ bacteria were dripped into the wound. 5 Common or robust *S. licheniformis* spore suspensions (spores / mL) were used, and each treatment without pathogen inoculation was inoculated with an equal volume of sterile water. *Bacillus subtilis* S12 and *Bacillus licheniformis* ST4 suspensions with a viable count of 0.1 billion / mL were used to replace the N8 suspension. The treated ginseng was incubated at 25°C under humidified conditions. After 14 days, the incidence of ginseng red skin disease was investigated, and the disease index and relative control efficacy were calculated.

[0053] Grading standards for ginseng red skin disease: Grade 0: Healthy ginseng roots without disease; Grade 1: 0% < relative area of ​​lesions on ginseng roots ≤ 5%; Grade 2: 5% < relative area of ​​lesions on ginseng roots ≤ 10%; Grade 3: 10% < relative area of ​​lesions on ginseng roots ≤ 30%; Grade 4: 30% < relative area of ​​lesions on ginseng roots ≤ 50%; Grade 5: Relative area of ​​lesions on ginseng roots > 50%. The relative area of ​​lesions on ginseng roots is the percentage of lesion area to the total root area.

[0054] Disease index = 100 × ∑ (number of disease roots at each level × value at each level) / (total number of disease roots investigated × highest level value).

[0055] Prevention and control effect = (disease index of treatment - disease index of blank control) × 100% / disease index of control. Results are shown below. Figure 4 . Figure 4 The results showed that the application of N8 under in vitro conditions could significantly reduce the severity of ginseng red skin disease caused by common or robust red skin, with control effects of 71.4% and 71.3%, respectively, which were significantly better than Bacillus subtilis S12 and Bacillus licheniformis ST4. Example 5: Field control effect of strain N8 on ginseng leaf spot and red skin disease.

[0056] An experiment was conducted in normal ginseng planting fields in Hongshi Township, Dunhua City, Jilin Province. Four experimental treatments were set up: Treatment 1 was based on conventional management; Treatment 2, in addition to conventional management, was sprayed with N8 inoculant at a rate of 2 liters / mu in late May, early July, and early August, respectively, using an N8 aqueous suspension with a viable count of 0.1 billion / mL. Treatments 3 and 4, using Bacillus subtilis S12 and Bacillus licheniformis ST4 aqueous suspensions with viable counts of 0.1 billion / mL, were used as controls to compare with N8. Each treatment was replicated in three plots, each plot measuring 1.8m × 5m. The occurrence of ginseng leaf spot disease was investigated in mid-August, and the occurrence of ginseng red skin disease was investigated in mid-September at harvest time. Disease index and relative control efficacy were calculated.

[0057] The survey was conducted as follows: All leaves of 10 ginseng plants in each plot were surveyed, and the total number of leaves and the number of diseased leaves at each level were recorded. The disease severity grading standards for ginseng leaf spot disease were as follows: Grade 0: leaves intact, no lesions; Grade 1: 0% < relative area of ​​leaf spots ≤ 5%; Grade 2: 5% < relative area of ​​leaf spots ≤ 10%; Grade 3: 10% < relative area of ​​ginseng root lesions ≤ 20%; Grade 4: 20% < relative area of ​​ginseng root lesions ≤ 50%; Grade 5: relative area of ​​ginseng root lesions > 50%. The relative area of ​​leaf spots is the percentage of lesion area to the total leaf area.

[0058] The investigation method for ginseng red skin disease was the same as in Example 4. The calculation methods for the disease index and prevention and control effect were the same as in Example 4.

[0059] See results Figure 5 . Figure 5 The results showed that applying N8 significantly reduced the incidence of ginseng leaf spot and red skin disease, with control effects of 81.0% and 70.6%, respectively, which were significantly better than Bacillus subtilis S12 and Bacillus licheniformis ST4. This indicates that N8 not only has a high control effect on ginseng leaf diseases, but also has the ability to induce disease resistance by applying it to the leaves to control root diseases. Example 6: Growth-promoting effect of strain N8

[0060] An experiment was conducted in normal ginseng planting fields in Hongshi Township, Dunhua City, Jilin Province. Four experimental treatments were set up: Treatment 1 was conventional management; Treatment 2, in addition to conventional management, was sprayed with N8 inoculant in late May, early July, and early August at a rate of 2 liters / mu (approximately 0.067 hectares), using an N8 aqueous suspension with a viable count of 0.1 billion CFU / mL as the foliage. Treatments 3 and 4, using Bacillus subtilis S12 and Bacillus licheniformis ST4 aqueous suspensions with viable counts of 0.1 billion CFU / mL as the foliage, were used for comparison with N8. Each treatment was replicated in three plots, each plot measuring 1.8 m × 5 m. At harvest time in mid-September, the aboveground fresh weight and root weight of the ginseng were investigated, with 10 ginseng plants investigated per plot. The root-to-shoot ratio and yield increase were calculated. Root-to-shoot ratio = root fresh weight / aboveground fresh weight. Root-to-shoot ratio increase = (Treatment area root-to-shoot ratio × 100%) / control area root-to-shoot ratio. Increase in production = (quality of processed single ginseng - quality of ginseng under normal management) × 100% / quality of ginseng under normal management.

[0061] See results Figure 6 . Figure 6 The results showed that applying N8 inoculant increased the number of fibrous roots and root hairs, and significantly improved the fresh weight of individual ginseng roots, resulting in a yield increase of up to 15.1%, which was significantly better than Bacillus subtilis S12 and Bacillus licheniformis ST4. Furthermore, the root-to-shoot ratio after applying N8 inoculant was 2.54±0.24, while the ratio of the conventional control was only 2.02±0.14, representing an increase of 25.6%. Example 7: Soil fertility enhancement function of strain N8

[0062] An experiment was conducted in normal ginseng planting fields in Hongshi Township, Dunhua City, Jilin Province. Four experimental treatments were set up: Treatment 1 was conventional management; Treatment 2, in addition to conventional management, was sprayed with N8 inoculant in late May, early July, and early August at a rate of 2 liters / mu (approximately 0.067 hectares), using an N8 aqueous suspension with a viable count of 0.1 billion CFU / mL as a foliar spray. Treatments 3 and 4, using Bacillus subtilis S12 and Bacillus licheniformis ST4 aqueous suspensions with viable counts of 0.1 billion CFU / mL, were used as comparisons with N8. Each treatment was replicated in three plots, each plot measuring 1.8 m × 5 m. Soil samples were collected from the rhizosphere of the ginseng in mid-September at harvest time, with five samples collected from each plot. These samples were sent to Nanjing Hanguang Testing Technology Co., Ltd. for analysis of soil organic matter, available nitrogen, and available potassium, and the nutrient enhancement was calculated. Nutrient improvement rate = (Nutrient content in the treatment area - Nutrient content in the conventional management area) × 100% / Nutrient content in the conventional management area.

[0063] See results Figure 7 . Figure 7The results showed that N8 inoculant treatment significantly increased soil organic matter, available nitrogen, and available potassium content, with significantly better results than Bacillus subtilis S12 and Bacillus licheniformis ST4. Compared with conventional management, N8 inoculant treatment increased soil organic matter content by 30.1%, available nitrogen content by 32.8%, and available potassium content by 5.8%.

Claims

1. Bacillus belye ( Bacillus velezensis The strain N8 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 36579.

2. A composition comprising the Bacillus belye strain N8 as described in claim 1, and an acceptable vector.

3. The Bacillus berberis strain N8 according to claim 1 or the composition according to claim 2 in the treatment of controlling red scab (… Ilyonectria ), Sclerotinia sclerotiorum ( Sclerotinia ), Botrytis cinerea ( Botrytis ), Alternaria ( Alternaria ), Rhizoctonia solani ( Rhizoctonia Fusarium ( ) Fusarium Anthrax bacteria ( Colletotrichum Application in at least one of the pathogens in ).

4. The application according to claim 3, characterized in that, The red crust mentioned is a common red crust ( Iyonectria communis ) and / or strong red crust ( Ilyonectria robusta ); and / or The *Sclerotinia sclerotiorum* is *Sclerotinia sclerotiorum* (… Sclerotinia sclerotiorum ) and / or neatly arranged sclerotia ( Sclerotinia rolfsii ); and / or The botrytis cinerea is Botrytis cinerea (Glaucus spp.) Botrytis cinerea ); and / or The *Rhizoctonia solani* is *Rhizoctonia solani* (… Rhizoctonia solani ); and / or The Fusarium is Fusarium oxysporum (Fusarium oxysporum) Fusarium oxysporum ) and / or Fusarium solani ( Fusarium solani ); and / or The anthrax bacterium is *Anthrax fructicus* ( ). Colletotrichum fructicola ).

5. The application according to claim 3 or 4, characterized in that, The Bacillus berberis strain N8 as described in claim 1 or the composition as described in claim 2 is used to prevent and control diseases of at least one of ginseng, American ginseng and Panax notoginseng.

6. The application according to any one of claims 3 to 5, characterized in that, Spray the above-ground parts of the target organism with the Bacillus berberis strain N8 as described in claim 1 or the composition as described in claim 2.

7. The use of the Bacillus berberis strain N8 according to claim 1 or the composition according to claim 2 in promoting growth and / or improving soil fertility.

8. The application according to claim 7, characterized in that, The soil fertility includes at least one of organic matter, available nitrogen, and available potassium.

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