Quantitative detection and analysis device for glycosylated hemoglobin

Abstract

The glycosylated hemoglobin quantitative detection and analysis device comprises a machine body, a detection area and a display control area are preset on the left side and the right side of the machine body, a stand column is installed in the detection area, the stand column is sleeved with a lower ring plate, and the stand column over the lower ring plate is sleeved with an upper ring plate; a plurality of mutually aligned placing holes are formed in the upper ring plate and the lower ring plate, a concave cavity in the radial direction is formed in the part, between the upper ring plate and the lower ring plate, of the stand column, a second movable plate and a first movable plate are installed on the concave cavity and the side face opposite to the concave cavity respectively, and bayonets aligned with the placing holes are formed in the second movable plate and the first movable plate; and an excitation light source and a photoelectric detector which are opposite to each other are respectively arranged in the movable plate II and the movable plate I. A sample is placed in the placing holes of the upper ring plate and the lower ring plate, during detection, the first movable plate and the second movable plate actively approach and surround the sample, a good internal light shielding environment is obtained, and external light is difficult to enter the interior to affect a detection result.

Description

Technical Field

[0001] This invention relates to the field of glycated hemoglobin detection, and more particularly to a device for quantitative detection and analysis of glycated hemoglobin. Background Technology

[0002] Enzymatic glycated hemoglobin (HbA1c) detection is a method that utilizes specific enzymes to specifically react with HbA1c, and quantifies HbA1c by detecting the reaction products. The core principle of enzymatic HbA1c detection is the specific recognition and reaction of specific enzymes with HbA1c. For example, enzymes such as glycated hemoglobin oxidase can specifically act on HbA1c, oxidizing and decomposing it to produce specific reaction products. These products can be quantitatively analyzed using colorimetry, fluorescence, or other detection methods to estimate the HbA1c content in the sample.

[0003] Currently, colorimetric detection of glycated hemoglobin requires an excitation light source emitting light of a specific wavelength, such as 700nm. After passing through the sample reaction system, the light is absorbed by the colorimetric H₂O₂, causing a change in the colorimetric intensity of the light received on the back side. Therefore, light is a crucial factor affecting the detection results. In current automated batch detection equipment, due to sample repositioning, gaps inevitably exist between components. Other light rays may pass through these gaps and be received by the photodetector, leading to errors in the actual results. Summary of the Invention

[0004] The technical problem to be solved by this invention is to overcome the defects of the existing technology. To solve the above technical problem, the technical solution adopted by this invention is: A glycated hemoglobin quantitative detection and analysis device includes a body with a detection area and a display and control area pre-set on the left and right sides of the body. A column is installed in the detection area, and a lower ring plate is sleeved on the column. An upper ring plate is sleeved on the column directly above the lower ring plate. Multiple aligned placement holes are opened on the upper and lower ring plates. A radial cavity is opened on the column between the upper and lower ring plates. A second movable plate and a first movable plate are respectively installed on the cavity and the side facing the cavity. The second movable plate and the first movable plate have a locking slot aligned with the placement hole. An excitation light source and a photodetector facing each other are respectively installed in the second movable plate and the first movable plate.

[0005] In this design, threaded rods are installed on the middle of the two sides of the movable plate inside the cavity and on the middle of the side of the movable plate away from the cavity. Threaded tubes are threaded onto the threaded rods. A limit sleeve is installed at the end of the threaded tube away from the threaded rod. The end of the threaded tube is rotated and limited within the limit sleeve. A fixed seat is installed inside the machine body at the same height as the first movable plate. The limit sleeves on the first and second movable plates are respectively installed on the column and the fixed seat. A gear ring two is fixed on the threaded tube. A gear two is installed on the outside of the gear ring two, and the gear two meshes with the gear ring two. A motor two is installed on the column and the fixed seat opposite to the gear two. The output end of the motor two is plugged into and connected to the gear two.

[0006] The cross-sections of both the second movable plate and the first movable plate are C-shaped, and side plates are installed on both the left and right sides of the first movable plate.

[0007] The lower ring plate has a toothed ring fixed at its bottom, and a gear is installed on the outside of the toothed ring. The gear meshes with the toothed ring. A motor is installed in the body directly below the gear, and the output end of the motor is plugged into the gear.

[0008] The side plate is hinged to the movable plate 1 via a hinge. A bracket is installed in the middle of the back of the movable plate 1. An electric cylinder is installed between the bracket and the side plate. Hinges are hinged to both ends of the electric cylinder. The hinges at both ends are installed with the bracket and the side plate.

[0009] An adhesive strip is installed on the inner side of the side plate corresponding to the middle position of the reagent tube.

[0010] A baffle is installed in the detection area outside the upper ring plate, and an aluminum-plated plate is installed on the outer side of the toothed ring below the baffle.

[0011] A cover plate is hinged to the body above the detection area via a hinge.

[0012] Among them, the four corners of the movable plate one and movable plate two on the outer side of the threaded rod are all fixed with sleeve rods, and the column and the fixed seat in the cavity are all installed with sleeves at the same height as the sleeve rod. One end of the sleeve rod extends into the sleeve to form a sliding sleeve connection.

[0013] Compared with the prior art, the beneficial effects of the present invention include: 1. In this invention, the sample is placed in the placement holes of the upper and lower ring plates. During the test, the movable plate one and the movable plate two actively approach and surround the sample to obtain a good internal light-proof environment, making it difficult for external light to enter the interior and affect the test results.

[0014] 2. In this invention, the sample is placed on the upper and lower ring plates and rotated by a gear, achieving the effect of controllable switching of the test sample. It can automatically test batches of samples without requiring manual operation.

[0015] 3. In this invention, a side plate is provided and an adhesive strip is provided on the side plate to block the gap between the side plate and the sample, so that the light from the excitation light source can only pass through the sample and be received by the photodetector, thereby improving the detection accuracy.

[0016] 4. In this invention, the side plate is rotated controllably by an electric cylinder. It can open to a certain angle during movement to avoid the adhesive strip directly contacting the sample. After it is in place, it is closed to ensure that the adhesive strip adheres to the sample.

[0017] 5. In this invention, the movement of the movable plate relies on the cooperation of the threaded rod and the threaded tube, as well as the drive of the motor and gears to achieve controllable rotation. It can surround the sample when testing each sample, and reset and leave after the test is completed, without affecting the entry of subsequent samples into the test position. Attached Figure Description

[0018] The disclosure of this invention is illustrated with reference to the accompanying drawings. It should be understood that the drawings are for illustrative purposes only and are not intended to limit the scope of protection of this invention. In the drawings, the same reference numerals are used to refer to the same parts. Wherein: Figure 1 This is a perspective view of the present invention; Figure 2 This is a cross-sectional view of the present invention; Figure 3 This is a schematic diagram of the reagent tube arrangement in this invention; Figure 4 This is a schematic diagram of the movable plate arrangement in this invention; Figure 5 This is a schematic diagram of the threaded rod configuration in this invention; Figure 6 This is a cross-sectional view of the column in this invention.

[0019] Reference numerals: 1. Body; 2. Detection area; 3. Display and control area; 4. Column; 5. Upper ring plate; 6. Lower ring plate; 7. Placement hole; 8. Gear ring one; 9. Support; 10. Gear one; 11. Motor one; 12. Cavity; 13. Movable plate one; 14. Movable plate two; 15. Side plate; 16. Bayonet; 17. Threaded tube; 18. Threaded rod; 19. Limiting sleeve; 20. Gear ring two; 21. Motor two; 22. Gear two; 23. Sleeve; 24. Sleeve rod; 25. Photodetector; 26. Excitation light source; 27. Adhesive strip; 28. Bracket; 29. ​​Electric cylinder; 30. Hinge seat; 31. Cover plate; 32. Fixed seat; 33. Support column; 34. Baffle; 35. Aluminum plate; 36. Reagent tube. Detailed Implementation

[0020] It is readily understood that, based on the technical solution of this invention, those skilled in the art can propose various interchangeable structural methods and implementations without altering the essential spirit of the invention. Therefore, the following detailed embodiments and accompanying drawings are merely illustrative examples of the technical solution of this invention and should not be considered as the entirety of the invention or as limitations or restrictions on the technical solution of this invention.

[0021] According to one embodiment of the present invention, Figures 1-6 As shown.

[0022] A glycated hemoglobin quantitative detection and analysis device includes a body 1. Detection areas 2 and display and control areas 3 are preset on the left and right sides of the body 1. A cover plate 31 is hinged to the body 1 above the detection areas 2 via a hinge. A column 4 is installed in the detection areas 2. A support 9 is welded and fixed to the bottom of the column 4. The support 9 is fixed to the body 1 at the bottom of the detection areas 2. A lower ring plate 6 is sleeved on the column 4 above the support 9. An upper ring plate 5 is sleeved on the column 4 directly above the lower ring plate 6. Multiple aligned placement holes 7 are opened on the upper ring plate 5 and the lower ring plate 6. A reagent tube 36 is placed in the placement holes 7. Multiple support columns 33 with equal spacing are welded and fixed to the bottom of the upper ring plate 5 and the top of the lower ring plate 6. A toothed ring 8 is welded and fixed to the bottom of the lower ring plate 6. A gear 10 is installed on the outside of the toothed ring 8. The gear 10 meshes with the toothed ring 8. A motor 11 is installed in the body 1 directly below the gear 10 by bolts. The output end of the motor 11 is plugged into the gear 10. A radial cavity 12 is provided on the column 4 between the upper ring plate 5 and the lower ring plate 6. Movable plate 2 14 and movable plate 13 are respectively installed on the cavity 12 and the side facing the cavity 12. The cross-section of movable plate 2 14 and movable plate 13 is C-shaped. A bayonet 16 aligned with the placement hole 7 is provided on movable plate 2 14 and movable plate 13. An excitation light source 26 and a photodetector 25 facing each other are respectively installed in movable plate 2 14 and movable plate 13. Side plates 15 are installed on both the left and right sides of movable plate 13. Threaded rods 18 are installed on the middle of the side of the movable plate 14 inside the cavity 12 and on the middle of the side of the movable plate 13 away from the cavity 12. Threaded tubes 17 are threadedly connected to the threaded rods 18. A limiting sleeve 19 is installed at the end of the threaded tube 17 away from the threaded rod 18. The end of the threaded tube 17 is rotated and limited within the limiting sleeve 19. A fixed seat 32 is installed inside the body 1 at the height aligned with the movable plate 13. The limiting sleeves 19 on the movable plate 13 and the movable plate 14 are respectively installed on the column 4 and the fixed seat 32. A gear ring 20 is welded and fixed to the threaded tube 17. A gear 22 is installed on the outside of the gear ring 20. The gear 22 meshes with the gear ring 20. A motor 21 is installed on the column 4 and the fixed seat 32 opposite to the gear 22. The output end of the motor 21 is plugged into the gear 22.

[0023] When using, open the cover 31 and add the reaction system formed by mixing peroxidase, fructovaline oxidase (FVO), chromogenic agent, other necessary buffers, cofactors, and treated whole blood or lysed blood into the reagent tube 36. Then place it in the placement hole 7, close the cover 31, and set the detection time from the display control area 3. The reaction system is first allowed to stand in the detection area 2, where it undergoes a natural reaction. Fructose valine oxidase (FVO) first catalyzes the oxidation of glycated valine to generate hydrogen peroxide (H2O2). Almost simultaneously, the peroxidase begins to catalyze the coupling reaction between hydrogen peroxide and the chromogenic agent to generate a colored compound. When the set detection time is reached, i.e., the reaction is complete, the detection is started. First, control motor 21 to work, so that its output end drives gear 22 to rotate. Since gear 22 and gear ring 20 are meshed, and gear ring 20 and threaded tube 17 are fixed, the operation of motor 21 eventually drives threaded tube 17 to rotate. Threaded tube 17 is threadedly connected to threaded rod 18. When threaded tube 17 is rotated and limited by limiting sleeve 19, threaded rod 18 moves in a linear motion with reference to threaded tube 17 as threaded tube 17 rotates, so that threaded rod 18 extends out of threaded tube 17. At the same time, it drives movable plate 13 and movable plate 2 to move closer to each other. When movable plate 13 and movable plate 2 can no longer move after they collide, they stop. At this time, the clamp 16 of movable plate 13 and movable plate 2 abuts against reagent tube 36 to form an enclosure. Then, the excitation light source 26 and the photodetector 25 are activated. When the excitation light source 26 is working, it emits a predetermined light beam. The photodetector 25 receives and detects the light information after passing through the reagent tube 36. According to the colorimetric determination, the absorbance value is proportional to the concentration of glycated hemoglobin. Therefore, the content of glycated hemoglobin in the sample can be calculated based on the detected absorbance value.

[0024] When a reagent tube 36 is tested, the test data is recorded. Then, motor 21 is reversed, driving movable plate 13 and movable plate 24 to reset, separating them from the tested reagent tube 36. After resetting, motor 11 is operated, causing its output to drive gear 10 to rotate. Gear 10 meshes with gear ring 8, while gear ring 8, lower ring plate 6, and upper ring plate 5 are fixed. Therefore, all reagent tubes 36 placed in the placement hole 7 are rotated around column 4 as the center, rotating by the interval of an adjacent placement hole 7, so that the next reagent tube 36 rotates to the testing position. At this time, motor 11 stops, and motor 21 is operated again. This cycle continues until all reagent tubes 36 are tested.

[0025] In this embodiment, see Figures 1-6 The side plate 15 is hinged to the movable plate 13 via a hinge. A bracket 28 is installed in the middle of the back of the movable plate 13. An electric cylinder 29 is installed between the bracket 28 and the side plate 15. Hinges 30 are hinged to both ends of the electric cylinder 29. The hinges 30 at both ends are installed with the bracket 28 and the side plate 15.

[0026] The C-shaped structure of movable plate 13 and movable plate 214 surrounds the reagent tube 36 from top to bottom. After setting the side plate 15, the reagent tube 36 can be surrounded circumferentially. With the upper ring plate 5 and lower ring plate 6 in the vertical direction, the reagent tube 36 to be tested can be surrounded in all directions, avoiding external light from entering and affecting the detection accuracy of the photodetector 25.

[0027] An adhesive strip 27 is installed on the inner side of the side plate 15, corresponding to the middle position of the reagent tube 36.

[0028] Because the plane of the side plate 15 is different from the curved structure of the reagent tube 36, and the side plate 15 cannot completely abut against the reagent tube 36, there is a large gap between the side plate 15 and the reagent tube 36. The light from the excitation light source 26 can pass through this gap and be directly received by the photodetector 25, thus affecting the detection accuracy of the photodetector 25. Therefore, the adhesive strip 27 is set to be attached to the reagent tube 36 to minimize the light leakage gap. When the movable plate 13 approaches the reagent tube 36, the electric cylinder 29 is first controlled to work, so that its output end pulls the side plate 15 to rotate around the hinge as the axis and open it outward at a certain angle to offset the position of the adhesive strip 27 and avoid pushing the reagent tube 36 when it enters in a straight line. When the movable plate 13 is in place, the electric cylinder 29 is controlled to extend, so that the side plate 15 is closed, and the adhesive strip 27 abuts against the reagent tube 36.

[0029] A baffle 34 is installed in the detection area 2 outside the upper ring plate 5. An aluminum plate 35 is installed on the outside of the toothed ring 8 below the baffle 34, with the aluminum plate surface of the aluminum plate 35 facing the reagent tube 36.

[0030] The baffle 34 and the aluminized plate 35 further divide the detection area 2. Together with the movable plate 13 and the movable plate 2 14 surrounding the reagent tube 36, and the cover plate 31 on the top of the detection area 2, the influence of external light sources on the detection results is minimized.

[0031] The four corners of the movable plate 13 and movable plate 14 on the outer side of the threaded rod 18 are welded and fixed with sleeve rods 24. The column 4 and the fixed seat 32 in the cavity 12 are both equipped with sleeves 23 at the same height as the sleeve rod 24. One end of the sleeve rod 24 extends into the sleeve 23 to form a sliding sleeve connection.

[0032] Movable plate 13 and movable plate 24 rely solely on the cooperation of threaded rod 18 and threaded tube 17 for support, resulting in poor support stability. Therefore, sleeve rod 24 and sleeve 23 are provided for auxiliary support. When movable plate 13 and movable plate 24 move, they drive sleeve rod 24 to slide within sleeve 23, providing support while allowing movement.

[0033] The technical scope of this invention is not limited to the content described above. Those skilled in the art can make various modifications and variations to the above embodiments without departing from the technical concept of this invention, and all such modifications and variations should fall within the protection scope of this invention.

Claims

1. A glycated hemoglobin quantitative detection and analysis device, comprising a body (1), wherein a detection area (2) and a display and control area (3) are preset on the left and right sides of the body (1), characterized in that, A column (4) is installed in the detection area (2). A lower ring plate (6) is fitted on the column (4). An upper ring plate (5) is fitted on the column (4) directly above the lower ring plate (6). Multiple placement holes (7) aligned with each other are opened on the upper ring plate (5) and the lower ring plate (6). A radial cavity (12) is opened on the column (4) between the upper ring plate (5) and the lower ring plate (6). Movable plate two (14) and movable plate one (13) are respectively installed on the cavity (12) and the side facing the cavity (12). A bayonet (16) aligned with the placement hole (7) is opened on the movable plate two (14) and movable plate one (13). An excitation light source (26) and a photodetector (25) facing each other are respectively installed in the movable plate two (14) and movable plate one (13).

2. The glycated hemoglobin quantitative detection and analysis device according to claim 1, characterized in that, Threaded rods (18) are installed on the middle of the side of the movable plate two (14) inside the cavity (12) and on the middle of the side of the movable plate one (13) away from the cavity (12). Threaded pipes (17) are threaded onto the threaded rods (18). A limiting sleeve (19) is installed at the end of the threaded pipe (17) away from the threaded rod (18). The end of the threaded pipe (17) is rotated and limited within the limiting sleeve (19). A fixed seat (32) is installed in the body (1) at the height aligned with the movable plate one (13). The limiting sleeves (19) on the movable plate 1 (13) and the movable plate 2 (14) are respectively installed on the column (4) and the fixed seat (32). The threaded pipe (17) is fixed with the toothed ring 2 (20). The toothed ring 2 (22) is installed on the outside of the toothed ring 2 (20). The toothed ring 2 (22) meshes with the toothed ring 2 (20). The column (4) and the fixed seat (32) opposite to the toothed ring 2 (22) are equipped with the motor 2 (21). The output end of the motor 2 (21) is plugged into the gear 2 (22).

3. The glycated hemoglobin quantitative detection and analysis device according to claim 2, characterized in that, The cross-sections of the second movable plate (14) and the first movable plate (13) are both C-shaped, and side plates (15) are installed on both the left and right sides of the first movable plate (13).

4. The glycated hemoglobin quantitative detection and analysis device according to claim 1, characterized in that, The bottom of the lower ring plate (6) is fixed with a toothed ring (8), and a gear (10) is installed on the outside of the toothed ring (8). The gear (10) meshes with the toothed ring (8). A motor (11) is installed in the body (1) directly below the gear (10). The output end of the motor (11) is plugged into the gear (10).

5. The glycated hemoglobin quantitative detection and analysis device according to claim 3, characterized in that, The side plate (15) is hinged to the movable plate (13) by a hinge. A bracket (28) is installed in the middle of the back of the movable plate (13). An electric cylinder (29) is installed between the bracket (28) and the side plate (15). Hinges (30) are hinged to both ends of the electric cylinder (29). The hinges (30) at both ends are installed with the bracket (28) and the side plate (15).

6. The glycated hemoglobin quantitative detection and analysis device according to claim 5, characterized in that, An adhesive strip (27) is installed on the inner side of the side plate (15) at the middle position of the reagent tube (36).

7. A glycated hemoglobin quantitative detection and analysis device according to claim 1 or 6, characterized in that, A baffle (34) is installed in the detection area (2) outside the upper ring plate (5), and an aluminum plate (35) is installed on the outside of the toothed ring (8) below the baffle (34).

8. The glycated hemoglobin quantitative detection and analysis device according to claim 7, characterized in that, A cover plate (31) is hinged to the body (1) above the detection area (2) via a hinge.

9. The glycated hemoglobin quantitative detection and analysis device according to claim 2, characterized in that, The four corners of the movable plate 1 (13) and movable plate 2 (14) on the outside of the threaded rod (18) are fixed with sleeve rods (24). The column (4) in the cavity (12) and the fixed seat (32) are both equipped with sleeves (23) at the same height as the sleeve rod (24). One end of the sleeve rod (24) extends into the sleeve (23) to form a sliding sleeve connection.

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