Compound termite killing composition containing thiotraniliprole

Abstract

The invention discloses a compound termite killing composition containing thiotraniliprole and a preparation method of the compound termite killing composition. The composition comprises the thiotraniliprole and the chlorpyrifos, wherein the weight ratio of the thiotraniliprole to the chlorpyrifos is (15: 1)-(1: 15). The co-toxicity coefficients of the thiotraniliprole and the chlorpyrifos in different compounding proportions are measured through a system, and the co-toxicity coefficients are gt when the weight ratio of the thiotraniliprole to the chlorpyrifos is 15: 1-1: 15; 120, the obvious synergism is shown; two indexes of termite survival rate and virus transmission efficiency are combined to further prove that the composition in the compound proportion range is good in termite control effect and high in virus transmission efficiency; the compound composition screened by the invention can efficiently prevent and control termites, overcomes the defects of high resistance and low prevention effect of a single agent, and can be popularized and used in production.

Description

Technical Field

[0001] This invention belongs to the field of termite control, specifically relating to a compound termite-killing composition containing thiocyanate. Background Technology

[0002] Thiorantraniliprole is a novel o-aminobenzamide insecticide independently developed by Zhejiang Yulong Biotechnology Co., Ltd. It acts on the ryanodine receptor in the insect body, causing excessive release of calcium ions in the cells, affecting the insect's feeding and ultimately leading to the paralysis and death of the target insect.

[0003] Chlorpyrifos is a broad-spectrum organophosphate insecticide that works primarily by inhibiting the activity of acetylcholinesterase (AChE) in insects, preventing them from breaking down acetylcholine. This leads to a large accumulation of neurotransmitters in the synaptic cleft, causing sustained excitation and spasms in the insect's nervous system, ultimately resulting in paralysis and death.

[0004] Termites, one of the world's five major pests, have become a global ecological problem due to their invasion and spread. Among the many termite species, the Formosan subterranean termite (C. formosanus) is one of the most damaging termite species due to its extremely strong dispersal ability and "soil-and-wood amphibious" survival characteristics. Currently, the control of Formosan subterranean termites mainly relies on chemical control. However, long-term use of chemical agents alone can lead to a decrease in efficacy, thus necessitating the selection of suitable compound combinations to improve control efficacy and reduce the dosage. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a compound termite-killing composition containing thiocyanate. On the one hand, it reduces the amount of pesticide used; on the other hand, from the perspective of the mechanism of action, the compositions have different mechanisms of action, eliminating cross-resistance and improving control efficacy.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] The first object of the present invention is to provide a termite-killing composition comprising thiocyanate and chlorpyrifos, wherein the weight ratio of thiocyanate to chlorpyrifos is 15:1 to 1:15.

[0008] Preferably, the weight ratio of thiophanate-methyl to chlorpyrifos is 15:1 to 1:3.

[0009] More preferably, the weight ratio of thiophanate-methyl to chlorpyrifos is 15:1.

[0010] A second object of the present invention is to provide a termite-killing formulation comprising the above-described termite-killing composition and a pesticide-acceptable carrier and adjuvant.

[0011] Preferably, the dosage form of the preparation is a powder, emulsifiable concentrate, water-dispersible granule, suspension concentrate, wettable powder, microemulsion, or water emulsion.

[0012] A third object of the present invention is to provide the use of the above-described termite-killing composition or termite-killing preparation in the prevention and control of termites.

[0013] A fourth object of the present invention is to provide a method for controlling termites, comprising the step of applying the above-described termite-killing composition or termite-killing agent.

[0014] Preferably, the application method includes powdering, spraying, pouring, impregnation, injection, or filling.

[0015] Beneficial effects:

[0016] This invention systematically measured the co-toxicity coefficients of thiophanate-methyl and chlorpyrifos at different compound ratios, and found that when the weight ratio of the two was 15:1-1:15, the co-toxicity coefficients were all >120, indicating a significant synergistic effect. Combined with the termite survival rate and the virus transmission efficiency, it was further confirmed that the composition within this compound ratio range has a good termite control effect and high virus transmission efficiency. The compound composition screened by this invention can effectively control termites, overcome the defects of high resistance and low efficacy of single agents, and can be promoted for use in production. Attached Figure Description

[0017] Figure 1 The survival rate of termites under different compounding ratios. Detailed Implementation

[0018] Those skilled in the art will understand that the techniques disclosed in the following embodiments represent those discovered by the inventors that work well in the practice of this invention. However, many changes can be made to the specific embodiments disclosed, still obtaining the same or similar results without departing from the spirit and scope of the invention.

[0019] Example 1

[0020] I. Experimental Methods

[0021] 1. Termite toxicity determination: Thionamide (CAS: 1442448-92-1) and chlorpyrifos (CAS: 2921-88-2) were purchased from Anpuyun Laboratory Supplies (Shanghai) Co., Ltd. Thionamide and chlorpyrifos were diluted to 5-6 different concentration gradients using acetone as a solvent. Specifically, thiofentanyl was diluted to 0.2, 1.0, 5.0, and 25.0 mg / L, and chlorpyrifos to 0.2266, 0.25, 0.625, 2.5, and 5.0 mg / L. Filter paper was then evenly moistened with 1 mL of the pesticide solution to ensure full absorption, and then air-dried. After drying, the filter paper was transferred to a clean petri dish, and 1 mL of sterile water and 50 healthy, uniform-sized Formosan subterranean termites were added. Mortality was observed after 24 hours. Each treatment was repeated in 5 replicates, with acetone as a control. Statistical results were analyzed using SPSS software to calculate the toxicity of the pesticides to the termites.

[0022] 2. Co-toxicity coefficient determination: Following the termite toxicity determination method, a compound insecticide was prepared by mixing thiophanate-methyl and chlorpyrifos at a mass ratio of 15:1-1:15 to treat termites. For each specific mass ratio, it was diluted to four final concentration gradients: 4.5, 0.7, 0.35, and 0 mg / L. The co-toxicity coefficient evaluation method was used to evaluate the effect of the compound insecticide on Formosan subterranean termites. The statistical results were analyzed using SPSS software to calculate the LC50 of the compound insecticide on termites. 50 Values. Based on Sun Yunpei's calculations of the drug's toxicity index and co-toxicity coefficient. A co-toxicity coefficient greater than 120 indicates a synergistic effect, a co-toxicity coefficient between 80 and 120 indicates an additive effect, and a co-toxicity coefficient less than 80 indicates an antagonistic effect.

[0023] 3. Survival rate determination: Insecticides were compounded according to a mass ratio of thiophanate-methyl to chlorpyrifos of 15:1-1:15. The LC ratios for each proportion are shown in Table 1. 50 Prepare the corresponding working solution using the baseline concentration. Evenly moisten filter paper with 1 ml of the compound insecticide, ensuring it fully absorbs the solution, and then allow it to air dry. Once dry, transfer the filter paper to a clean petri dish, add 1 ml of sterile water and healthy, uniformly sized Formosan subterranean termites. After 24 hours, select 50 healthy, vigorous termites and place them in a new, clean petri dish. Observe termite mortality starting from the second day, repeating every two days, with five replicates per treatment.

[0024] 4. Virus transmission efficiency determination: Healthy Formosan subterranean termites of uniform size were selected and fed 1% Brilliant Blue edible pigment for 6-7 days to stain and mark the termites. Then, they were treated with a compound insecticide (the compounding ratio and concentration were the same as in the survival rate determination experiment above). Four hours after staining, 10 healthy termites were selected and placed in a new, clean petri dish. Then, 10 untreated healthy termites were added to the petri dish at a 1:1 ratio. Termite mortality was observed starting from the second day, with observations every 2 days and 5 replicates per treatment.

[0025] II. Experimental Results

[0026] The results of the co-toxicity coefficient determination of thiofenoxam and chlorpyrifos at different compound ratios showed that the co-toxicity coefficient of thiofenoxam and chlorpyrifos was greater than 120 when the compound ratio was in the range of 15:1-1:15, indicating a synergistic effect (Table 1).

[0027] Table 1. Determination of co-toxicity coefficient between thiophanate-methyl and chlorpyrifos (for Formosan subterranean termites)

[0028] Statistical analysis of termite mortality data from different compound ratios of thiofenoxam and chlorpyrifos revealed that the survival time of termites treated with all compound ratios was longer than that of the chlorpyrifos-only treatment group, but shorter than that of the thiofenoxam-only treatment group. Figure 1 ).

[0029] Statistical analysis of termite transmission efficiency data from different ratios of thiofenoxam and chlorpyrifos revealed that, compared to the control group, all treatments except for those using chlorpyrifos alone, thiofenoxam, and chlorpyrifos at ratios of 1:9 and 1:15 showed significant differences compared to the control group. Furthermore, compared to treatments using thiofenoxam and chlorpyrifos alone, only a 15:1 ratio of thiofenoxam to chlorpyrifos showed significant transmission efficiency, indicating that this ratio was more effective at transmitting termites than either chlorpyrifos or thiofenoxam alone (Table 2).

[0030] Table 2. Termite transmission ability to compound pesticides under different compound ratios (specifically for Formosan subterranean termites).

[0031] Note: Cumulative mortality data (mean ± SEM), different lowercase letters indicate significant differences at the P=0.05 level.

[0032] in conclusion:

[0033] The above results indicate that when the ratio of thiophanate-methyl to chlorpyrifos is 15:1, the compound has a synergistic effect and can significantly improve termite mortality and virus transmission capacity. Therefore, termite control can be achieved by mixing thiophanate-methyl and chlorpyrifos in a 15:1 ratio.

Claims

1. A termite-killing composition, characterized in that, It contains thiocyanate and chlorpyrifos, wherein the weight ratio of thiocyanate to chlorpyrifos is 15:1 to 1:

15.

2. The termite-killing composition according to claim 1, characterized in that, The weight ratio of thiophanate-methyl to chlorpyrifos is 15:1 to 1:

3.

3. The termite-killing composition according to claim 2, characterized in that, The weight ratio of thiofentanil to chlorpyrifos is 15:

1.

4. A termite-killing agent, characterized in that, The formulation comprises the termite-killing composition according to any one of claims 1-3 and a pesticide-acceptable carrier and adjuvant.

5. The termite-killing agent according to claim 4, characterized in that, The dosage form of the preparation is powder, emulsifiable concentrate, water-dispersible granules, suspension concentrate, wettable powder, microemulsion, or water emulsion.

6. Use of the termite-killing composition according to any one of claims 1-3 or the termite-killing agent according to any one of claims 4-5 in the prevention and control of termites.

7. A method for controlling termites, characterized in that, The step includes applying the termite-killing composition according to any one of claims 1-3 or the termite-killing agent according to any one of claims 4-5.

8. The method according to claim 7, characterized in that, The application methods include powdering, spraying, pouring, soaking, injection, or filling.

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