Gene influencing precocious puberty of pigs in deep county and screening method thereof
By analyzing differentially expressed genes in the testicular tissue of Shenxian pigs through transcriptome sequencing, SRD5A1 and CYP11B2 were screened out, which solved the problem that traditional breeding methods could not accurately utilize the precocious puberty trait of Shenxian pigs, and improved breeding efficiency and reproductive efficiency.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-02-03
- Publication Date
- 2026-04-21
AI Technical Summary
Traditional breeding methods are difficult to accurately identify and efficiently utilize the precocious puberty trait in Shenxian pigs, and are also affected by environmental factors, impacting breeding efficiency and cost.
By analyzing differentially expressed genes in the testicular tissue of Shenxian pigs through transcriptome sequencing, candidate genes SRD5A1 and CYP11B2 related to sexual maturation were screened out. Combined with gene function analysis, key genes affecting precocious puberty in Shenxian pigs were screened out.
This study provides a gene screening method for precocious puberty in Shenxian pigs, laying the foundation for subsequent research, improving breeding efficiency and accuracy, reducing the maintenance cost of boars, and enhancing reproductive efficiency.
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Abstract
Description
Technical Field
[0001] This invention belongs to the field of pig sexual maturation research, and in particular relates to genes that affect precocious puberty in Shenxian pigs and their screening methods. Background Technology
[0002] Currently, livestock breeding is at a critical stage of technological revolution and breeding paradigm iteration. A technological wave centered on genomics, biotechnology, and information technology is driving the transition from traditional "experience-based selection" to intelligent "precision design." Genome-wide selection has become a core tool in commercial breeding, significantly accelerating genetic progress; cutting-edge technologies such as gene editing provide effective pathways for rapidly introducing superior traits; and the deep integration of artificial intelligence and big data has built a "smart breeding" platform, enabling precise prediction and optimization of complex traits, injecting core momentum into improving breeding efficiency.
[0003] The high-quality development of the industry presents diversified and comprehensive challenges to breeding technologies. Breeding must balance production efficiency with the regulation of complex traits. It requires not only breaking through traditional breeding bottlenecks through technological innovation, but also carrying out precise improvements on traits such as meat flavor, disease resistance, and environmental adaptability to meet market demands for high-quality and distinctive livestock and poultry products, driving the transformation of breeding towards refinement and efficiency. Against the backdrop of increasingly fierce international competition in breeding technologies, building an independent and controllable breeding technology system and overcoming the technical barriers to the regulation of complex traits has become crucial to enhancing core competitiveness in breeding.
[0004] Shenxian pigs, as an important local pig breed resource, possess unique advantages such as strong adaptability, high disease resistance, early sexual maturity, and excellent meat quality (Yang Shan, Li Sai, Cao Hongzhan. Development of germplasm resources of Shenxian pigs [J]. Pig Science, 2025, 42(04):36-38.). Boars can ejaculate at 3 months of age, and sows can become pregnant and give birth at 4 months of age, making them a typical precocious breed. The precocious sexual maturity characteristics generally exhibited by its boars (i.e., early puberty, strong libido, and strong mating ability) are excellent traits with significant economic value. This trait can effectively shorten the generation interval, accelerate the breeding process, reduce the maintenance cost of breeding boars, and improve the reproductive efficiency of sows, which is of great significance for increasing efficiency and reducing costs in the commercial pig production system. Due to the scarcity of Shenxian pigs, this characteristic can be used to breed more Shenxian pigs, and Shenxian pigs can also be used to carry out some crossbreeding to develop new breeds. As a distinctive local breed, the Shenxian pig can be better raised by studying its precocious sexual maturity patterns and molecular mechanisms. It can also be used as a hybrid parent to inherit its excellent qualities.
[0005] However, precocious puberty is a complex quantitative trait regulated by multiple genes. Traditional breeding methods, based on phenotype selection, are time-consuming, inefficient, and susceptible to environmental interference, making it difficult to accurately identify and efficiently utilize this superior trait. Therefore, in-depth research based on cutting-edge breeding technologies is urgently needed. Currently, no research has been reported on the genes responsible for precocious puberty in Shenxian pigs. Summary of the Invention
[0006] In view of this, the purpose of this invention is to provide genes affecting precocious puberty in Shenxian pigs and their screening methods. This invention performs transcriptome sequencing on testicular tissues of Shenxian pigs before and after sexual maturity, analyzes the function of differentially expressed genes, and screens candidate genes related to sexual maturity in Shenxian pigs. Then, transcriptome sequencing is performed on testicular tissues of sexually mature Shenxian pigs and sexually mature Shenxian pig × Large White pig crossbred pigs to analyze the function of differentially expressed genes and screen for genes specifically expressed during sexual maturity in Shenxian pigs. The results of the two transcriptome sequencing studies are combined to analyze the function of overlapping genes, and candidate genes SRD5A1 and CYP11B2 affecting precocious puberty in Shenxian pigs are screened based on gene function; this lays the foundation for subsequent research on precocious puberty in Shenxian pigs.
[0007] This invention provides genes that influence precocious puberty in Shenxian pigs, including the SRD5A1 gene and the CYP11B2 gene.
[0008] This invention provides the application of the aforementioned gene in the study of precocious puberty in Shenxian pigs.
[0009] This invention provides a method for screening genes that affect precocious puberty in Shenxian pigs, comprising the following steps: 1) Transcriptome sequencing was performed on testicular tissues of Shenxian pigs before and after sexual maturity. Differentially expressed genes before and after sexual maturity were analyzed, and candidate genes related to sexual maturity of Shenxian pigs were screened out. 2) Transcriptome sequencing was performed on testicular tissues from sexually mature Shenxian pigs and sexually mature Shenxian pig × Large White pig hybrids to analyze differentially expressed genes and screen out candidate genes. 3) After taking the intersection of the candidate genes selected in step 1) and the candidate genes selected in step 2), analyze the gene function to obtain the genes that affect the precocious puberty of Shenxian pigs.
[0010] Preferably, step 3) involves analyzing gene function, including GO functional enrichment and KEGG path analysis.
[0011] Preferably, after obtaining the gene affecting precocious puberty in Shenxian pigs in step 3), the method further includes using q-PCR to verify the relative expression levels of the gene affecting precocious puberty in Shenxian pigs before and after sexual maturity.
[0012] This invention also provides the application of the genes that affect precocious puberty in Shenxian pigs in early assisted breeding of Shenxian pigs, screening individuals with significantly high expression levels of the SRD5A1 gene and / or CYP11B2 gene.
[0013] Compared with existing technologies, this invention has the following advantages: This invention employs transcriptome sequencing. First, transcriptome sequencing is performed on testicular tissues of Shenxian pigs before and after sexual maturity to analyze differentially expressed genes and screen for candidate genes related to sexual maturity in Shenxian pigs. Then, transcriptome sequencing is performed on testicular tissues of sexually mature Shenxian pigs and crossbred pigs of sexually mature Shenxian pigs × Large White pigs to analyze the function of differentially expressed genes and screen for specific expression genes of sexually mature Shenxian pigs. This invention combines the results of the two transcriptome sequencing studies to analyze the function of overlapping genes and screen for genes SRD5A1 and CYP11B2 that influence precocious puberty in Shenxian pigs based on gene function, laying the foundation for subsequent research on precocious puberty in Shenxian pigs. Attached Figure Description
[0014] Figure 1 Correlation analysis was performed among samples of sexually mature Shenxian pigs S2 and pre-sexually mature Shenxian pigs S1. Figure 2 Principal component analysis was performed among samples of sexually mature Shenxian pig S2 and pre-sexually mature Shenxian pig S1. Figure 3 A statistical diagram showing the differentially expressed genes between the sexually mature Shenxian pig S2 group and the pre-sexually mature Shenxian pig S1 group; Figure 4 Volcano diagram of differentially expressed genes in sexually mature Shenxian pig S2 and pre-sexually mature Shenxian pig S1; Figure 5 Cluster analysis heatmap of sexually mature Shenxian pigs S2 and pre-sexually mature Shenxian pigs S1; Figure 6 GO functional enrichment maps of sexually mature Shenxian pig S2 and pre-sexually mature Shenxian pig S1; Figure 7 KEGG pathway analysis diagrams for sexually mature Shenxian pigs S2 and pre-sexually mature Shenxian pigs S1; Figure 8 q-PCR validation of differentially expressed genes in sexually mature Shenxian pig S2 and pre-sexually mature Shenxian pig S1; Figure 9 Correlation analysis was performed among samples of sexually mature hybrid pigs YS and sexually mature Shenxian pigs S. Figure 10 PCA analysis was performed among samples of sexually mature hybrid pig YS and sexually mature Shenxian pig S. Figure 11 Statistical analysis of differentially expressed genes in sexually mature hybrid pigs YS and S pigs S; Figure 12Volcano plot of differentially expressed genes in sexually mature hybrid pig YS and sexually mature Shenxian pig S; Figure 13 Clustering heatmap of differentially expressed genes in sexually mature hybrid pigs YS and S; Figure 14 GO functional enrichment diagram for sexually mature hybrid pig YS and sexually mature Shenxian pig S; Figure 15 KEGG pathway analysis diagrams for sexually mature hybrid pigs YS and S Shenxian pigs; Figure 16 q-PCR validation of differentially expressed genes in sexually mature hybrid pigs YS and S pigs S. Detailed Implementation
[0015] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0016] Example 1
[0017] (I) Transcriptome sequencing of testicular tissue from boars in Shenxian County before and after sexual maturity
[0018] This experiment selected boars from different litters of Shenxian pigs from Hebei Zhengnong Animal Husbandry Co., Ltd. Three 6-month-old boars of the same weight from different litters were randomly selected from those not selected for breeding. Similarly, three 1-month-old boars of the same weight from different litters were selected from newly weaned boars. The non-sexually mature boars were designated as the control group (S2), and the sexually mature boars as the experimental group (S1). Testicular tissue was removed by physical castration, cut along the midline, and a soybean-sized sample was taken from the middle portion. After washing with PBS buffer, the sample was placed in cryovials and immediately immersed in liquid nitrogen for preservation. RNA was extracted and transcribed into DNA for sequencing in the laboratory.
[0019] First, RNA was extracted, then the RNA concentration was detected, and the RNA integrity was checked by gel electrophoresis. After passing the test, library construction and sequencing analysis were performed.
[0020] Tissue samples were extracted using the TRNzol method. After extraction, RNA concentration, RIN value, 28S / 18S ratio, and fragment size were measured using an Agilent 2100 Bioanalyzer (USA, Agilent Technologies, Inc.) to determine RNA integrity. RNA purity (OD260 / 280) was measured using a NanoDrop UV spectrophotometer (USA, Thermo Scientific).
[0021] mRNA was enriched from total RNA using Oligo dT magnetic beads. After fragmentation, first-strand cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis. After end repair, A-tailing, adapter ligation, fragment selection, amplification, and purification, the library was ready. Samples that passed the initial screening were sequenced using a DNBSEQ-T7 (China, MGI, Inc) sequencer. The raw data were then quality-controlled and compared with a reference genome. The FPKM of each gene was calculated based on its length, and the readings mapped to that gene were calculated. Finally, differential expression analysis was performed, and GO functional enrichment and KEGG pathway analysis were conducted on differentially expressed genes.
[0022] The results are shown in Table 1. A total of 43.4 Gb of clean bases were obtained from transcriptome sequencing, with Q30 ranging from 92.86% to 94.43% and GC content from 46.18% to 48.43%. As shown in Table 2, comparing the clean bases with the reference gene, the sequencing accuracy of each sample was above 95%, and the clean read accuracy ranged from 91.48% to 92.37%. The clean bases obtained after filtering the raw bases were no less than 6.64 Gb, with Q20 > 97.49%, Q30 > 92.86%, and GC content > 46.18%, indicating high-quality transcriptome sequencing data suitable for subsequent research.
[0023] Table 1. Summary of sequencing data for samples S1 and S2
[0024] Table 2. Comparison of reference genes between samples S1 and S2.
[0025] like Figure 1 As shown, the correlations among the samples in each group are significant. Figure 2 As shown, the samples in both groups clustered in the same location and were clearly distributed, meeting the criteria for further analysis.
[0026] like Figure 3 As shown, a total of 11,033 differentially expressed genes were screened, of which 6,016 were upregulated and 5,017 were downregulated. The distribution of differentially expressed genes in the volcano plot is shown below. Figure 4 As shown in the figure. Union and intersection differentially expressed genes were extracted from multiple groups of differential results, and cluster heatmap analysis was performed using the FPKM values of each sample. The results are shown in the figure. Figure 5 As shown, there are significant differences in gene distribution between groups, while genes within groups are basically similar.
[0027] GO functional enrichment of differentially expressed genes mainly focuses on three parts: biological process (BP), cellular component (CC), and molecular function (MF). A bar chart is created by selecting the top 10 significant functional items in each category, as shown below. Figure 6 As shown, this gene set exhibits systematic functional enrichment during testicular maturation and spermatogenesis. It not only directly participates in core biological processes such as reproduction and sexual reproduction but is also enriched in ciliary tissue and motility, cell population proliferation, and other aspects, collectively supporting spermatogenesis and cell renewal. At the structural level, the genes are highly concentrated in cilia, sperm flagella, and microtubule-related cellular components, indicating their crucial role in the assembly of sperm tail structure and motility apparatus. Simultaneously, the enrichment of molecular functions such as protein kinase activity, ATP binding, and microtubule binding further reveals the kinase signal transduction, energy metabolism, and cytoskeleton dynamic regulation mechanisms upon which this process depends. These findings collectively demonstrate that this gene set comprehensively supports the cellular structure, proliferation regulation, and signaling network basis required for spermatogenesis, flagellar formation, and functional realization during testicular maturation at the transcriptional level.
[0028] In the KEGG signaling pathway analysis of differentially expressed genes, the top 20 significant pathways were selected and plotted in a bubble chart, as shown below. Figure 7 As shown in the figure, enrichment analysis of this group of genes at the pathway level revealed that key signaling networks related to testicular maturation and spermatogenesis mainly include the MAPK signaling pathway, the PI3K-Akt signaling pathway, the Rap1 signaling pathway, and the regulation of the actin cytoskeleton. These pathways collectively regulate the proliferation, differentiation, survival, and structural remodeling of spermatogenic cells. Furthermore, the enrichment of apoptosis pathways reflects the regulatory mechanisms of selective cell clearance during spermatogenesis, while cellular senescence pathways may be involved in maintaining germ cell homeostasis. Although some pathways in the list are associated with viral infection or disease, some, such as attachment plaques, phospholipase D signaling pathways, and axonal guidance, also play roles in cell adhesion, membrane dynamics, and morphogenesis, indirectly supporting cell migration and structural assembly during spermatogenesis. In summary, these pathways systematically constitute the molecular regulatory basis for spermatogenesis and testicular functional maturation at multiple levels, including signal transduction, cytoskeleton dynamics, cell cycle, and cell death.
[0029] RNA was extracted again from the same samples, and suitable differentially expressed genes were screened for quantitative fluorescence analysis. TPB was used as an internal control gene, and 2... -ΔΔCt The algorithm calculates the relative expression level of the target gene and compares it with the sequencing data. Primer amplification information is shown in Table 3.
[0030] Table 3 Primer sequence information for S1 and S2 quantitative PCR amplification
[0031] Using S2 (before sexual maturity) as the control group and S1 (after sexual maturity) as the experimental group, quantitative real-time PCR was performed for verification. TPB As an internal reference gene, 2 -ΔΔCt The algorithm calculates the relative expression levels of the target gene and compares the significance with the transcriptome sequencing results, then performs a bar chart analysis. For example... Figure 8 As shown, the results revealed TSSK6 SAPA16, CFAPA43, and TX101 were all significantly increased. FOLR2 , ITGA6 , ERBB3 and SYT10 All were significantly downregulated, consistent with transcriptome sequencing results.
[0032] (II) Transcriptome sequencing of testicular tissue from sexually mature Shenxian pigs and Shenxian Large White pig crossbred boars
[0033] The differentially expressed genes screened in the above experiments were mainly enriched in reproduction and sperm, and no genes were found that significantly regulate hormone levels and affect precocious puberty. Therefore, testicular tissue was collected from three 6-month-old Shenxian pig × Large White pig hybrids selected at the same time as the above experimental pig herd. The hybrid pigs were used as the control group and designated as group YS, while the Shenxian pigs were used as the experimental group and designated as group S. RNA was extracted from both groups and subjected to transcriptome sequencing.
[0034] As shown in Table 4, a total of 39.21 Gb of clean bases were obtained from transcriptome sequencing, with Q30 ranging from 97.57% to 97.75% and GC content from 49.8% to 50.47%. As shown in Table 4, comparing the clean bases with the reference gene, the sequencing alignment of each sample was above 97%, and the accuracy of clean reads ranged from 94.83% to 95.04%. The clean bases obtained after filtering the raw bases were no less than 6.14 Gb, with Q20 > 99.36%, Q30 > 97.57%, and GC content > 49.80%, indicating high-quality transcriptome sequencing data suitable for subsequent research.
[0035] Table 4 Summary of sequencing data for samples YS and S
[0036] Table 5. Comparison of reference gene results between samples YS and S.
[0037] like Figure 9 As shown, the correlations among samples within each group are all significant. Figure 10The samples within each group are clustered, while those between groups are dispersed, allowing for further analysis.
[0038] like Figure 11 As shown, a total of 702 differentially expressed genes were screened, of which 582 were upregulated and 120 were downregulated. The differentially expressed gene volcano is shown in the figure. Figure 12 As shown. Figure 13 As shown, there are differences in gene distribution between groups, while the genes within groups are basically similar.
[0039] GO functional enrichment of differentially expressed genes mainly focused on three areas: biological process (BP), cellular component (CC), and molecular function (MF). A bar chart was created by selecting the top 10 significant functional entries in each category. For example... Figure 14 As shown, the functional enrichment characteristics of this gene set are highly consistent with the regulation of sexual maturation, with its core pointing to the synthesis, secretion, and signal transduction network of sex hormones. In biological processes, these genes are significantly enriched in steroid biosynthesis, hormone metabolism, and hormone level regulation, directly related to the production and homeostasis of key steroid hormones such as testosterone and estrogen, which drive sexual maturation. Molecularly, the enrichment of oxidoreductase activity and heme binding provides the catalytic basis for the cytochrome P450 enzyme system upon which steroid hormone synthesis depends, while G protein-coupled peptide receptor activity suggests its responsiveness to peptide signaling molecules such as gonadotropins, together constituting a key link in hormone synthesis and reception. Furthermore, the enrichment of cellular components on the cell surface, apical plasma membrane, and lysosomes further demonstrates the cellular basis of this gene set's involvement in the transmembrane transport, polar secretion, and metabolic reabsorption of hormone precursors. These functions, from hormone biosynthesis, oxidative modification, signal recognition to subcellular localization, systematically depict an endocrine regulatory program supporting gonadal activation, secondary sexual characteristic development, and the initiation of reproductive function.
[0040] In the KEGG signaling pathway analysis of differentially expressed genes, the top 20 significant pathways were selected and plotted as a bubble chart. For example... Figure 15As shown, the pathway enrichment analysis results of this gene group are highly consistent with the core regulatory mechanisms of sexual maturation, mainly focusing on the synthesis and metabolism network of gonadal steroid hormones. The core pathways "ovarian steroid production" and "steroid biosynthesis" directly point to the key event driving sexual maturation—the biosynthesis of sex hormones. Meanwhile, the enrichment of the "cortisol synthesis and secretion" pathway suggests that the hypothalamic-pituitary-gonadal axis and stress axis may interact and regulate each other, jointly influencing the timing of puberty initiation. To achieve efficient hormone synthesis, the genes are also enriched in multiple supporting metabolic pathways, including alanine, aspartate, and glutamate metabolism, valine, leucine, and isoleucine degradation, and fatty acid degradation. These pathways are provided with energy and precursors by catabolism; while cofactor biosynthesis and retinol metabolism provide essential coenzymes and vitamin A derivatives for related synthetic enzymes (such as cytochrome P450). In summary, these pathways, from core hormone synthesis, substrate and energy supply, cofactor support to cellular environmental adaptation, systematically constitute the complete metabolic and signaling basis supporting gonadal activation and secondary sexual characteristics development at multiple levels.
[0041] Using YS hybrid pigs as the control group and S Shenxian pigs as the experimental group, quantitative real-time PCR was performed for verification. TPB As an internal reference gene, 2 -ΔΔCt The algorithm calculates the relative expression level of the target gene. For example... Figure 16 As shown, the results revealed INSL3 , CYP11B2 , SRD5A1 , ARSE , AIG1 , GPX1 , ATG4A and GUSB All were upregulated, consistent with the transcriptome sequencing results, thus verifying the accuracy of the transcriptome sequencing results.
[0042] Gene analysis that may affect precocious puberty in Shenxian pigs
[0043] Table 6 Primer sequence information for S and YS real-time PCR amplification
[0044] In the first transcriptome sequencing, most of the screened genes were related to sperm function in Shenxian pigs, while genes associated with precocious puberty showed little expression. In the second sequencing, it became clear that most of the screened genes were related to hormone regulation and might affect precocious puberty in Shenxian pigs. Therefore, the results of the two sequencing analyses were combined, and all upregulated genes were analyzed. A total of 39 differentially expressed genes were found to be significantly upregulated in Shenxian pigs. The names and functions of all genes are shown in Table 7.
[0045] By searching the NCBI database for the functions of these 39 genes, 32 of them had clearly studied functions. Combining the results of GO functional enrichment and KEGG path analysis with relevant literature reports, the functions of these 32 genes were analyzed. Two genes most likely to influence precocious puberty were identified. SRD5A1 And LOC110260194 (i.e. CYP11B2 ). SRD5A1 Its main function is to catalyze the formation of dihydrotestosterone from testosterone, and the early accumulation of dihydrotestosterone can promote the early appearance of secondary sexual characteristics, i.e., precocious puberty. CYP11B2 belong CYP This family is involved in the synthesis of sex hormones. CYP11B2 It plays a role in the synthesis of adrenocortical hormones, and by affecting the body's hormone balance, it may indirectly regulate the onset time of puberty.
[0046] Table 7. Genes with overlapping sequencing results
[0047] In this experiment, 39 differentially expressed genes specific to Shenxian pigs were screened through two transcriptome sequencing analyses. Of these, 7 lacked functional annotation, leaving 32. After functional analysis, these genes were further screened. SRD5A1 and CYP11B2 This is a gene that may influence precocious puberty in boars from Shenxian County. In existing studies, SRD5A1 It primarily catalyzes the conversion of testosterone into the more potent dihydrotestosterone, playing a crucial role in regulating androgen metabolism. SRD5A1 Genetic abnormalities or defects can cause androgen imbalances, and even lead to disease. It's not difficult to observe this process. SRD5A1 By activating testosterone and affecting androgens, the occurrence of precocious puberty is influenced, and this effect was observed in Shenxian pigs in this experiment. SRD5A1 Gene expression levels were all at significant levels, therefore... SRD5A1 The gene was identified as influencing precocious puberty in pigs from Shenxian County. CYP11B2 It belongs to the cytochrome P450 family, which is widely involved in the synthesis and metabolism of androgens. CYP11B2 Due to CYP11B1 Highly homologous, unequal allele exchange during meiosis can lead to the formation of CYP11B2 / CYP11B1 chimera, CYP11B2 In chimeras, this is a promoter and is not regulated by corticotropin-releasing hormone, leading to cortisol deficiency and 11β-hydroxylase deficiency (11β-OHD), clinically manifested as precocious puberty. Therefore... CYP11B2It is also a candidate gene that affects precocious puberty in Shenxian pigs.
[0048] It was known while reading the literature that... KISS Related to the initiation of puberty (Li Huazhen, Chu Mingxing. Research progress on the regulation of mammalian reproductive performance by KISS1R gene [J]. Chinese Journal of Herbivorous Animal Science, 2021, 41(03):48-53.; Xing Feng, Liao Qiuping. Research progress on genes related to the initiation of puberty in mammals [J]. Journal of Animal Ecology, 2017, 38(07):1-4.), but in this experiment, KISS The gene expression level was not high, and it was not selected as a candidate gene. Besides... KISS Besides the relevant genes, different breeds also have different genes for precocious puberty. In Cao Haoming's (Cao Minghao. Molecular germplasm analysis of Hanjiang Black Pig and mining of key genes for precocious puberty [D]. Northwest A&F University, 2024.) study on Hanjiang Black Pig, [the following is mentioned:] PAPPA2 The gene is considered to influence precocious puberty in Hanjiang black pigs, but in this experiment... PAPPA2 There was no significant difference in gene expression levels, so it was not selected as a candidate gene. This demonstrates that there are significant varietal differences in the screening of genes for precocious puberty.
[0049] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.
Claims
1. A gene influencing precocious puberty in Shenxian pigs, characterized by, This includes the SRD5A1 gene and / or the CYP11B2 gene.
2. The application of the gene described in claim 1 in the study of precocious puberty in Shenxian pigs.
3. A method for screening genes affecting precocious puberty in Shenxian pigs, characterized in that, Includes the following steps: 1) Transcriptome sequencing was performed on testicular tissues of Shenxian pigs before and after sexual maturity. Differentially expressed genes before and after sexual maturity were analyzed, and candidate genes related to sexual maturity of Shenxian pigs were screened out. 2) Transcriptome sequencing was performed on testicular tissues from sexually mature Shenxian pigs and sexually mature Shenxian pig × Large White pig hybrids to analyze differentially expressed genes and screen out candidate genes. 3) After taking the intersection of the candidate genes selected in step 1) and the candidate genes selected in step 2), analyze the gene function to obtain the genes that affect the precocious puberty of Shenxian pigs.
4. The screening method according to claim 3, characterized in that, Step 3) The gene function analysis includes GO functional enrichment and KEGG path analysis.
5. The screening method according to claim 3, characterized in that, Step 3) After obtaining the gene that affects the precocious puberty of Shenxian pigs, the method of q-PCR is used to detect the relative expression level of the gene that affects the precocious puberty of Shenxian pigs before and after sexual maturity for verification.
6. The application of the gene affecting precocious puberty in Shenxian pigs as described in claim 1 in early assisted selection of Shenxian pigs, characterized in that, Individuals with significantly high expression levels of the SRD5A1 gene and / or CYP11B2 gene were screened.