Fattening sheep compound premix for regulating intestinal flora and preparation method thereof

Abstract

The invention discloses a fattening sheep compound premix for regulating intestinal flora and a preparation method thereof, and each kilogram of the compound premix consists of the following components in parts by weight: 80-120g of a micro-ecological regulation component, 20-30g of an amino acid composition, 5-10g of compound vitamins, 30-50g of compound minerals, 15-25g of a Chinese herbal medicine extract and 745-850g of a carrier, according to the fattening sheep compound premix capable of regulating the intestinal flora and the preparation method of the fattening sheep compound premix, regulation and control are performed through probiotics, prebiotics and Chinese herbal medicine extracts, lactobacillus plantarum and bacillus subtilis can directionally proliferate intestinal beneficial bacteria and inhibit growth of harmful bacteria such as escherichia coli and staphylococcus aureus, and saccharomyces cerevisiae culture assists in regulating intestinal microecological balance, so that the intestinal flora can be regulated and controlled, and the intestinal flora can be regulated and controlled. The galactomannan oligosaccharide is used as prebiotics and can promote colonization and proliferation of probiotics and prolong the action time of the probiotics in intestinal tracts, and the Chinese herbal medicine extract and the micro-ecological components cooperate to assist in regulating the intestinal function and enhance the immunity of the organism.

Description

Technical Field

[0001] This invention relates to the field of animal feed technology, specifically to a compound premix for fattening sheep that regulates intestinal flora and its preparation method. Background Technology

[0002] The fattening period is a crucial stage for sheep, characterized by the fastest growth rate and the most concentrated nutritional needs. The stability of their intestinal micro-ecosystem directly determines the efficiency of nutrient digestion and absorption, the body's immunity, and the economic benefits of sheep farming. Currently, sheep fattening operations commonly suffer from problems such as unreasonable premix nutrient ratios and intestinal flora imbalance. Specifically, this manifests as an overgrowth of harmful bacteria (such as Escherichia coli and Staphylococcus aureus) and an insufficient number of beneficial bacteria (such as Lactobacillus and Bifidobacterium) in the sheep's intestines, leading to abnormal intestinal motility, decreased digestive and absorptive functions, diarrhea, low feed utilization, and poor daily weight gain. Furthermore, while some premixes contain antibiotics to inhibit harmful bacteria, long-term use can lead to drug resistance and drug residues in meat, violating the principles of green farming and disrupting the natural balance of the intestinal flora, which is detrimental to the healthy fattening of sheep.

[0003] In existing technologies, although some compound premixes for fattening sheep have attempted to add traditional Chinese medicine or single probiotics to regulate the gut, there are obvious shortcomings: First, the traditional Chinese medicine formulas are not very targeted, focusing more on immune enhancement, with weak targeted regulation of the gut microbiota, and they do not form a synergistic effect with nutrients; Second, the probiotic strains are not reasonably selected, have poor tolerance, and are easily inactivated in feed preparation, storage, and the gastrointestinal environment of meat sheep, thus failing to play an effective role; Third, the ratio of nutrients and microecological regulators is unbalanced, either ignoring the role of vitamins and trace elements in intestinal mucosal repair, or the amount of probiotics added is too high or too low, making it difficult to achieve a long-term balance of the gut microbiota.

[0004] Therefore, developing a scientifically formulated and highly targeted compound premix that can regulate the intestinal flora structure of fattening sheep, inhibit the reproduction of harmful bacteria, promote the proliferation of beneficial bacteria, supplement the nutrients required during the fattening period, improve growth performance, and is free of drug residues and green and safe, as well as its preparation method, has become a technical problem that the current fattening sheep breeding industry urgently needs to solve. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a compound premix for fattening sheep that regulates intestinal flora and its preparation method. This invention solves the problems of poor intestinal flora regulation, easy inactivation of probiotics, unreasonable nutrient ratio, and risk of drug residues in existing premixes, thereby achieving a balance of intestinal microecology, improved growth performance, and green farming in fattening sheep.

[0006] To achieve the above objectives, the present invention provides the following technical solution: A compound premix for fattening sheep that regulates intestinal flora, wherein each kilogram of compound premix consists of the following components in parts by weight: Microecological regulating components 80-120g, amino acid combination 20-30g, compound vitamins 5-10g, compound minerals 30-50g, Chinese herbal extracts 15-25g, carrier 745-850g; The microecological regulating component is composed of *Lactobacillus plantarum*, *Bacillus subtilis*, *Saccharomyces cerevisiae* culture, and galactomannan oligosaccharide in a weight ratio of 3:2:4:1, wherein the viable count of *Lactobacillus plantarum* is ≥1×10⁻⁶. 10 CFU / g, Bacillus subtilis viable count ≥1×10⁻⁶ 10 CFU / g, viable count of Saccharomyces cerevisiae culture ≥1×10⁻⁶ 9 CFU / g; The amino acid combination is composed of methionine, lysine, and threonine in a weight ratio of 5:3:2. The compound vitamin contains per 100g: Vitamin A 400,000-500,000 IU, Vitamin D3 50,000-70,000 IU, Vitamin E 1400-1600 IU, Vitamin B1 20-30mg, Vitamin B2 30-40mg, Vitamin B... 12 0.1-0.2mg, nicotinic acid 150-200mg, pantothenic acid 80-100mg; The compound mineral contains per 100g: 40-50g ferrous methionine, 1-2g copper methionine, 20-22g zinc methionine, 20-25g manganese glycine, 1-2g iodine glycine, 0.6-1.0g selenium glycine, and 1.0-1.4g cobalt glycine. The herbal extract is prepared by mixing hawthorn, tangerine peel, astragalus, and honeysuckle in a weight ratio of 4:2:2:1, followed by water extraction and alcohol precipitation, with a solid content ≥60%. The carrier is made of stone powder and corn cob powder mixed in a weight ratio of 1:1, with a moisture content of ≤12% and a particle size of 80-100 mesh.

[0007] Preferably, each kilogram of the compound premix consists of the following components in parts by weight: 100g of microecological regulating components, 25g of amino acid combination, 8g of compound vitamins, 40g of compound minerals, 20g of traditional Chinese medicine extracts, and 797g of carrier; The weight ratio of *Lactobacillus plantarum*, *Bacillus subtilis*, *Saccharomyces cerevisiae* culture, and galactomannan oligosaccharide in the microecological regulating component is 3:2:4:1, and the viable count of *Lactobacillus plantarum* is ≥1×10⁻⁶. 10 CFU / g, Bacillus subtilis viable count ≥1×10⁻⁶ 10CFU / g, viable count of Saccharomyces cerevisiae culture ≥1×10⁻⁶ 9 CFU / g; The compound vitamin contains per 100g: Vitamin A 450,000 IU, Vitamin D 3 60,000 IU, Vitamin E 1,500 IU, Vitamin B1 25mg, Vitamin B2 35mg, Vitamin B... 12 0.15mg, nicotinic acid 180mg, pantothenic acid 90mg; The compound mineral contains the following per 100g: 45.6g ferrous methionine, 1.4g copper methionine, 21.4g zinc methionine, 22.6g manganese glycine, 1.5g iodine glycine, 0.8g selenium glycine, and 1.2g cobalt glycine. The herbal extract has a solid content of ≥65%, the carrier has a moisture content of ≤10%, and a particle size of 100 mesh.

[0008] Furthermore, the *Lactobacillus plantarum* is *Lactobacillus plantarum* CGMCC No. 1.557, and the *Bacillus subtilis* is *Bacillus subtilis* CGMCC No. 1.1086. Both strains are highly resistant and can survive in the acidic environment of the sheep's gastrointestinal tract, and have a significant inhibitory effect on harmful bacteria. The *Saccharomyces cerevisiae* culture is prepared by special *Saccharomyces cerevisiae* strains through liquid aerobic fermentation and solid anaerobic fermentation, followed by concentration and drying, which can further enhance the effect of intestinal microbial balance regulation.

[0009] Further, the preparation method of the herbal extract is as follows: Hawthorn, dried tangerine peel, astragalus, and honeysuckle are washed, pulverized, and passed through a 60-mesh sieve. Distilled water is added at a material-to-liquid ratio of 1:15 (g / mL), and the mixture is extracted in an 80℃ water bath for 2 hours. After filtration, the residue is added to distilled water at a material-to-liquid ratio of 1:10 (g / mL), and extracted again in an 80℃ water bath for 1 hour. The two filtrates are combined, concentrated under reduced pressure to 1 / 5 of the original volume, and 95% ethanol is added until the ethanol volume fraction reaches 70%. The mixture is allowed to stand for 24 hours, centrifuged, and the precipitate is collected, vacuum dried, pulverized, and passed through an 80-mesh sieve to obtain the herbal extract. This method can fully extract the active ingredients from the herbal extracts. Hawthorn and dried tangerine peel can improve feed palatability and enhance gastrointestinal motility; astragalus can improve the body's immunity; and honeysuckle has heat-clearing and detoxifying effects. The four ingredients work synergistically to help regulate intestinal function and inhibit the growth of harmful bacteria.

[0010] A method for preparing a compound premix for fattening sheep that regulates intestinal flora includes the following steps: S1. Raw material pretreatment: S1.1: Place the carrier (stone powder, corn cob powder) into a dryer and dry it at 60-70℃ until the moisture content is ≤12%. After pulverizing, pass it through an 80-100 mesh sieve for later use. S1.2: The cultures of Lactobacillus plantarum, Bacillus subtilis, and Saccharomyces cerevisiae in the microecological regulation component are pulverized and passed through an 80-mesh sieve, and then mixed evenly with galactomannan oligosaccharide for later use; S1.3: Grind the amino acid combination, compound vitamins, and compound minerals separately through an 80-mesh sieve and set aside; S1.4: Check the solid content of the herbal extract to ensure it is ≥60%, then pulverize it through an 80-mesh sieve and set it aside. S2, Premixed: Take 10%-20% of the total amount of carrier after step S1, put it into a small mixer, add the microecological regulation components, amino acid combination, compound vitamins and compound minerals after step S1, mix for 10-15 minutes at a speed of 200-300 r / min to obtain premix; this step adopts the "small amount carrier premixing" mode, which can avoid the agglomeration of trace components and ensure the uniformity of mixing.

[0011] S3, Total Mixing: The premix obtained in step S2 is placed in a large mixer, and the remaining carrier and the Chinese herbal extracts processed in step S1 are added. The mixture is mixed for 20-30 minutes at a speed of 150-200 r / min to ensure that the coefficient of variation of the mixing uniformity is <5% to obtain a mixed powder. S4. Conditioning and Granulation (Optional): If preparing granular premix, steam is introduced into the mixed powder obtained in step S3, the temperature is adjusted to 80-90℃ and the pressure to 0.3-0.5MPa, the moisture content of the powder is controlled to be 14%-16%, and then fed into a ring die pellet mill with a die diameter of 3-5mm and a pelletizing temperature of 75-85℃ to obtain granules; if preparing powdered premix, it can directly proceed to step S5. S5. Cooling and screening: The powdered premix obtained in step S3 or the granules obtained in step S4 are fed into a countercurrent cooler and cooled to room temperature +5℃ or below. The moisture content is controlled to be ≤12%. The granules need to be screened by a vibrating screen to remove broken particles (screen mesh size 2.5-4mm) to obtain the finished composite premix. S6. Packaging and Storage: The finished compound premix obtained in step S5 is vacuum-packed in 25kg / bag or 50kg / bag format and stored in a cool, dry, and ventilated warehouse at a temperature ≤25℃ and relative humidity ≤60% for a shelf life of 6 months. Vacuum packaging can effectively prevent the inactivation of microecological components and extend the shelf life of the premix.

[0012] Compared with the prior art, the present invention provides a compound premix for fattening sheep that regulates intestinal flora and its preparation method, which has the following beneficial effects: 1. This compound premix for fattening sheep that regulates intestinal flora and its preparation method involves regulation through probiotics, prebiotics, and traditional Chinese medicine extracts. Lactobacillus plantarum and Bacillus subtilis can directionally proliferate beneficial intestinal bacteria and inhibit the growth of harmful bacteria such as Escherichia coli and Staphylococcus aureus. Saccharomyces cerevisiae culture helps regulate the balance of intestinal microecology. Galactomannan oligosaccharide, as a prebiotic, can promote the colonization and proliferation of probiotics and prolong their action time in the intestine. The synergistic effect of traditional Chinese medicine extracts and microecological components can not only help regulate intestinal function but also enhance the body's immunity. No antibiotics are needed, avoiding drug residues and drug resistance, which meets the requirements of green farming.

[0013] 2. This compound premix for fattening sheep that regulates intestinal flora and its preparation method, through the combination of amino acids, compound vitamins, and compound minerals, provides methionine, lysine, and threonine to supplement the essential amino acids required for the growth of fattening sheep and improve the utilization rate of feed protein; the compound vitamins A, D3, and E can protect the intestinal mucosa and enhance immunity, while B vitamins can promote nutrient metabolism; the compound minerals are organically chelated, with high absorption rate, which can meet the mineral requirements of fattening sheep for rapid growth, while avoiding the adverse effects of inorganic minerals on intestinal flora. Detailed Implementation

[0014] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0015] Example 1

[0016] A compound premix for fattening sheep that regulates gut microbiota, comprising the following components in parts by weight per kilogram: 80g of microecological regulating components, 20g of amino acid combination, 5g of compound vitamins, 30g of compound minerals, 15g of traditional Chinese medicine extract, and 750g of carrier; Among them, the microecological regulation component is: Lactobacillus plantarum (CGMCC No. 1.557, viable count 1×10⁻⁶). 10 24g of CFU / g, Bacillus subtilis (CGMCC No. 1.1086, viable count 1×10⁻⁶) 10 16g (CFU / g), 32g of brewer's yeast culture, and 8g of galactomannan oligosaccharides; Amino acid combination: 10g methionine, 6g lysine, 4g threonine; Multivitamins (per 100g): Vitamin A 400,000 IU, Vitamin D3 50,000 IU, Vitamin E 1,400 IU, Vitamin B1 20 mg, Vitamin B2 30 mg, Vitamin B... 12 0.1mg, nicotinic acid 150mg, pantothenic acid 80mg; Complex minerals (per 100g): 40g ferrous methionine, 1g copper methionine, 20g zinc methionine, 20g manganese glycine, 1g iodine glycine, 0.6g selenium glycine, 1.0g cobalt glycine; Traditional Chinese medicine extract: hawthorn, tangerine peel, astragalus, and honeysuckle were mixed in a ratio of 4:2:2:1 and prepared by water extraction and alcohol precipitation, with a solid content of 60%. Carrier: 375g stone powder, 375g corn cob powder, moisture content 12%, particle size 80 mesh.

[0017] Preparation method: S1. Raw material pretreatment: Place stone powder and corn cob powder into a dryer and dry at 60℃ until the moisture content is 12%, then pulverize them through an 80-mesh sieve; pulverize Lactobacillus plantarum, Bacillus subtilis, and Saccharomyces cerevisiae cultures through an 80-mesh sieve respectively, and mix them evenly with galactomannan oligosaccharides; pulverize the amino acid combination, compound vitamins, and compound minerals through an 80-mesh sieve respectively; pulverize the traditional Chinese medicine extracts through an 80-mesh sieve, and set aside. S2. Premix: Take 10% (75g) of the total carrier and put it into a small mixer. Add the microecological regulation components, amino acid combination, compound vitamins and compound minerals. Mix at 200r / min for 10min to obtain the premix. S3. Overall mixing: Place the premix into a large mixer, add the remaining carrier (675g) and the herbal extract, mix at 150r / min for 20min, the coefficient of variation of the mixing uniformity is <5%, and obtain the powdered premix. S4. Cooling and sieving: Cool the powdered premix to room temperature, control the moisture content to ≤12%, and sieve to remove impurities; S5. Packaging and Storage: Vacuum packaged, 25kg / bag, stored in a warehouse at a temperature below 25℃ and a relative humidity of ≤60%.

[0018] Example 2

[0019] A compound premix for fattening sheep that regulates gut microbiota, comprising the following components in parts by weight per kilogram: 100g of microecological regulating components, 25g of amino acid combination, 8g of compound vitamins, 40g of compound minerals, 20g of traditional Chinese medicine extracts, and 797g of carrier; Among them, the microecological regulation component is: Lactobacillus plantarum (CGMCC No. 1.557, viable count 1×10⁻⁶). 1030g of CFU / g, Bacillus subtilis (CGMCC No. 1.1086, viable count 1×10⁻⁶) 10 20g (CFU / g), 40g brewer's yeast culture, 10g galactomannan oligosaccharide; Amino acid composition: methionine 12.5g, lysine 7.5g, threonine 5g; Multivitamins (per 100g): Vitamin A 450,000 IU, Vitamin D3 60,000 IU, Vitamin E 1,500 IU, Vitamin B1 25 mg, Vitamin B2 35 mg, Vitamin B... 12 0.15mg, nicotinic acid 180mg, pantothenic acid 90mg; Complex minerals (per 100g): Ferrous methionine 45.6g, Copper methionine 1.4g, Zinc methionine 21.4g, Manganese glycine 22.6g, Iodine glycine 1.5g, Selenium glycine 0.8g, Cobalt glycine 1.2g; Traditional Chinese medicine extract: hawthorn, tangerine peel, astragalus, and honeysuckle were mixed in a ratio of 4:2:2:1 and prepared by water extraction and alcohol precipitation, with a solid content of 65%. Carrier: 398.5g stone powder, 398.5g corn cob powder, moisture content 10%, particle size 100 mesh.

[0020] Preparation method: S1. Raw material pretreatment: Place stone powder and corn cob powder into a dryer and dry at 65℃ until the moisture content is 10%, then pulverize them through a 100-mesh sieve; pulverize Lactobacillus plantarum, Bacillus subtilis, and Saccharomyces cerevisiae cultures through an 80-mesh sieve, and mix them evenly with galactomannan oligosaccharides; pulverize the amino acid combination, compound vitamins, and compound minerals through an 80-mesh sieve; pulverize the traditional Chinese medicine extracts through an 80-mesh sieve, and set aside. S2. Premix: Take 15% (119.55g) of the total carrier and put it into a small mixer. Add the microecological regulation components, amino acid combination, compound vitamins and compound minerals. Mix at 250r / min for 12min to obtain the premix. S3. Overall mixing: Place the premix into a large mixer, add the remaining carrier (677.45g) and the herbal extract, mix at 180r / min for 25min, and the coefficient of variation of the mixing uniformity is <5% to obtain the mixed powder. S4. Conditioning and Granulation: Steam is introduced into the mixed powder at a temperature of 85℃ and a pressure of 0.4MPa to adjust the moisture content to 15%. The mixture is then fed into a ring die pellet mill with a die diameter of 4mm and a pelletizing temperature of 80℃ to obtain granules. S5. Cooling and screening: The granular material is fed into a counter-current cooler and cooled to room temperature +3℃. The moisture content is controlled at 11%. The material is then screened by a vibrating screen to remove broken particles. The screen mesh size is 3mm. S6. Packaging and storage: Vacuum packaged, 50kg / bag, stored in a warehouse at a temperature below 20℃ and a relative humidity of ≤55%.

[0021] Example 3

[0022] A compound premix for fattening sheep that regulates gut microbiota, comprising the following components in parts by weight per kilogram: Microecological regulation components 120g, amino acid combination 30g, compound vitamins 10g, compound minerals 50g, Chinese herbal extracts 25g, carrier 765g; Among them, the microecological regulation component is: Lactobacillus plantarum (CGMCC No. 1.557, viable count 1×10⁻⁶). 10 36g of CFU / g, Bacillus subtilis (CGMCC No. 1.1086, viable count 1×10⁻⁶) 10 24g (CFU / g), 48g of brewer's yeast culture, and 12g of galactomannan oligosaccharides; Amino acid combination: methionine 15g, lysine 9g, threonine 6g; Multivitamins (per 100g): Vitamin A 500,000 IU, Vitamin D3 70,000 IU, Vitamin E 1,600 IU, Vitamin B1 30 mg, Vitamin B2 40 mg, Vitamin B... 12 0.2mg, nicotinic acid 200mg, pantothenic acid 100mg; Complex minerals (per 100g): 50g ferrous methionine, 2g copper methionine, 22g zinc methionine, 25g manganese glycine, 2g iodine glycine, 1.0g selenium glycine, 1.4g cobalt glycine; Traditional Chinese medicine extract: hawthorn, tangerine peel, astragalus, and honeysuckle were mixed in a ratio of 4:2:2:1 and prepared by water extraction and alcohol precipitation, with a solid content of 70%. Carrier: 382.5g stone powder, 382.5g corn cob powder, moisture content 8%, particle size 100 mesh.

[0023] Preparation method: S1. Raw material pretreatment: Place stone powder and corn cob powder into a dryer and dry at 70℃ to 8% moisture content, then pulverize through a 100-mesh sieve; pulverize Lactobacillus plantarum, Bacillus subtilis, and Saccharomyces cerevisiae cultures through an 80-mesh sieve, and mix them evenly with galactomannan oligosaccharides; pulverize amino acid combinations, compound vitamins, and compound minerals through an 80-mesh sieve; pulverize the herbal extracts through an 80-mesh sieve, and set aside. S2. Premix: Take 20% (153g) of the total carrier and put it into a small mixer. Add the microecological regulation components, amino acid combination, compound vitamins and compound minerals. Mix at 300r / min for 15min to obtain the premix. S3. Overall mixing: Place the premix into a large mixer, add the remaining carrier (612g) and the herbal extract, mix at 200r / min for 30min, and the coefficient of variation of the mixing uniformity is <5% to obtain the mixed powder. S4. Conditioning and Granulation: Steam is introduced into the mixed powder at a temperature of 90℃ and a pressure of 0.5MPa to adjust the moisture content to 16%. The mixture is then fed into a ring die pellet mill with a die diameter of 5mm and a pelletizing temperature of 85℃ to obtain granules. S5. Cooling and screening: The granular material is fed into a counter-current cooler and cooled to room temperature +4℃. The moisture content is controlled at 10%. The material is then screened by a vibrating screen to remove broken particles. The screen mesh size is 4mm. S6. Packaging and storage: Vacuum packaged, 25kg / bag, stored in a warehouse at a temperature below 18℃ and a relative humidity of ≤50%.

[0024] Comparative test

[0025] One hundred and twenty fattening sheep of similar weight (25±2kg) and health status were randomly divided into four groups of 30 sheep each: control group, Example 1 group, Example 2 group, and Example 3 group. All groups were kept under the same feeding conditions (basic feed formula, feeding environment, feed amount, and management method). The experiment lasted for 60 days. Daily weight gain, feed conversion ratio, and diarrhea incidence were recorded for each group. Intestinal flora were measured after the experiment, and the results are shown in the table below.

[0026] The experimental results showed that, compared with the control group, the daily weight gain of fattening sheep in the 1-3 groups of the present invention was significantly increased (15%-23%), the feed conversion ratio was significantly decreased (10%-17%), and the incidence of diarrhea was significantly reduced (80%-90%). The number of lactic acid bacteria in the intestines was significantly increased (3.8-5.2 times), and the number of Escherichia coli was significantly reduced (70%-84%). This indicates that the compound premix of the present invention can effectively regulate the intestinal flora structure of fattening sheep, promote the proliferation of beneficial bacteria, inhibit the growth of harmful bacteria, and significantly improve the growth performance of fattening sheep. Among them, the 2 group of the present invention showed the best effect.

[0027] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A compound premix for fattening sheep that regulates intestinal flora, characterized in that: Each kilogram of compound premix consists of the following components by weight: 80-120g of microecological regulation components, 20-30g of amino acid combination, 5-10g of compound vitamins, 30-50g of compound minerals, 15-25g of traditional Chinese medicine extracts, and 745-850g of carrier. The microecological regulating component is composed of Lactobacillus plantarum, Bacillus subtilis, Saccharomyces cerevisiae culture, and galactomannan oligosaccharide in a weight ratio of 3:2:4:1, wherein the viable count of Lactobacillus plantarum is ≥1×10⁻⁶. 10 CFU / g, Bacillus subtilis viable count ≥1×10⁻⁶ 10 CFU / g, viable count of Saccharomyces cerevisiae culture ≥1×10⁻⁶ 9 CFU / g; The amino acid combination is composed of methionine, lysine, and threonine in a weight ratio of 5:3:

2. The compound vitamin contains per 100g: Vitamin A 400,000-500,000 IU, Vitamin D3 50,000-70,000 IU, Vitamin E 1,400-1,600 IU, Vitamin B1 20-30mg, Vitamin B2 30-40mg, Vitamin B... 12 0.1-0.2mg, nicotinic acid 150-200mg, pantothenic acid 80-100mg; The compound mineral contains per 100g: 40-50g ferrous methionine, 1-2g copper methionine, 20-22g zinc methionine, 20-25g manganese glycine, 1-2g iodine glycine, 0.6-1.0g selenium glycine, and 1.0-1.4g cobalt glycine. The herbal extract is prepared by mixing hawthorn, tangerine peel, astragalus, and honeysuckle in a weight ratio of 4:2:2:1, followed by water extraction and alcohol precipitation, with a solid content ≥60%. The carrier is made of stone powder and corn cob powder mixed in a weight ratio of 1:1, with a moisture content of ≤12% and a particle size of 80-100 mesh.

2. The compound premix for fattening sheep that regulates intestinal flora according to claim 1, characterized in that: Each kilogram of compound premix consists of the following components by weight: 100g of microecological regulation components, 25g of amino acid combination, 8g of compound vitamins, 40g of compound minerals, 20g of traditional Chinese medicine extracts, and 797g of carrier. The compound vitamin contains per 100g: Vitamin A 450,000 IU, Vitamin D 3 60,000 IU, Vitamin E 1,500 IU, Vitamin B1 25mg, Vitamin B2 35mg, Vitamin B... 12 0.15mg, nicotinic acid 180mg, pantothenic acid 90mg; The compound mineral contains the following per 100g: 45.6g ferrous methionine, 1.4g copper methionine, 21.4g zinc methionine, 22.6g manganese glycine, 1.5g iodine glycine, 0.8g selenium glycine, and 1.2g cobalt glycine. The herbal extract has a solid content of ≥65%, the carrier has a moisture content of ≤10%, and a particle size of 100 mesh.

3. The compound premix for fattening sheep that regulates intestinal flora according to claim 1, characterized in that: The *Lactobacillus plantarum* is *Lactobacillus plantarum* CGMCC No. 1.557, and the *Bacillus subtilis* is *Bacillus subtilis* CGMCC No. 1.1086; the *Saccharomyces cerevisiae* culture is prepared by special *Saccharomyces cerevisiae* through liquid aerobic fermentation and solid anaerobic fermentation, followed by concentration and drying.

4. The compound premix for fattening sheep that regulates intestinal flora according to claim 1, characterized in that: The preparation method of the herbal extract is as follows: Hawthorn, tangerine peel, astragalus, and honeysuckle are washed, crushed, and passed through a 60-mesh sieve. Distilled water is added at a material-to-liquid ratio of 1:15 (g / mL), and the mixture is extracted in an 80℃ water bath for 2 hours. After filtration, the residue is added to distilled water at a material-to-liquid ratio of 1:10 (g / mL), and the mixture is extracted again in an 80℃ water bath for 1 hour. The two filtrates are combined, concentrated under reduced pressure to 1 / 5 of the original volume, and 95% ethanol is added until the ethanol volume fraction is 70%. The mixture is allowed to stand for 24 hours, centrifuged, and the precipitate is vacuum dried, crushed, and passed through an 80-mesh sieve to obtain the extract.

5. A method for preparing a compound premix for fattening sheep that regulates intestinal flora according to any one of claims 1-4, characterized in that, Includes the following steps: S1. Raw material pretreatment: Dry the carrier to a moisture content of ≤12% and pulverize it through an 80-100 mesh sieve; pulverize each strain in the microecological regulation component through an 80 mesh sieve and mix it evenly with galactomannan oligosaccharide; pulverize the amino acid combination, compound vitamins, and compound minerals through an 80 mesh sieve; pulverize the Chinese herbal medicine extract through an 80 mesh sieve and set aside. S2. Premixing: Take 10%-20% of the total amount of carrier after step S1, add microecological regulation components, amino acid combination, compound vitamins and compound minerals, mix for 10-15 minutes at a speed of 200-300 r / min to obtain premix; S3. Overall mixing: Mix the premix with the remaining carrier and herbal extracts for 20-30 minutes at a speed of 150-200 r / min, with a mixing uniformity variation coefficient of <5%, to obtain a mixed powder. S4. Cooling and sieving: Cool the mixed powder to room temperature +5℃, control the moisture content to ≤12%, and sieve to remove impurities to obtain the finished product; if preparing granular premix, add a conditioning and granulation step after step S3, and then perform cooling and sieving. S5. Packaging and Storage: Vacuum pack the finished product and store it in a cool, dry, and well-ventilated warehouse.

6. The compound premix for fattening sheep that regulates intestinal flora according to claim 5, and its preparation method, characterized in that: In step S1, the carrier drying temperature is 60-70℃; in step S4, the specific parameters for conditioning and granulation are: introducing steam to adjust the temperature to 80-90℃ and the pressure to 0.3-0.5MPa, controlling the moisture content of the powder to 14%-16%, feeding it into a ring die granulator with a die diameter of 3-5mm and a granulation temperature of 75-85℃.

7. The compound premix for fattening sheep that regulates intestinal flora according to claim 1, and its preparation method, characterized in that: In step S4, a counter-current cooler is used for cooling, and a vibrating screen is used for screening the granular material with a screen aperture of 2.5-4mm.

8. The compound premix for fattening sheep that regulates intestinal flora according to claim 1, and its preparation method, characterized in that: In step S5, the packaging specifications are 25kg / bag or 50kg / bag, the storage conditions are temperature ≤25℃, relative humidity ≤60%, and the shelf life is 6 months.

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