Steam explosion cotton stalk microorganism composite ecological restoration material and production method thereof
By combining steam explosion treatment and nano-TiO2 modification with microbial fermentation, a stable three-dimensional network structure is formed, which solves the problem of poor performance of traditional ecological restoration materials and achieves efficient ecological restoration and waste resource utilization.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- XINJIANG HONGYIRUN BIOTECHNOLOGY CO LTD
- Filing Date
- 2026-01-22
- Publication Date
- 2026-04-28
AI Technical Summary
Traditional ecological restoration materials do not promote plant growth and have poor restoration effects, making it difficult to effectively improve soil degradation and water loss problems.
Cotton stalks are treated using a continuous steam explosion device, combined with acid or alkali impregnation and nano-TiO2 modification, and fermentation with fermenting bacteria to form a microbial composite ecological restoration material. Through chemical bonding and microbial fermentation, a stable three-dimensional network structure is formed, which enhances the mechanical properties and restoration efficiency of the material.
It significantly improves the mechanical strength and structural stability of the material, promotes plant root growth, enhances the remediation efficiency of complex polluted environments, extends the service life of the material, and realizes the resource utilization of waste.
Smart Images
Figure CN121930848A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of resource utilization technology, specifically to a steam-exploded cotton stalk microbial composite ecological restoration material and its production method. Background Technology
[0002] In recent years, with the acceleration of industrialization and urbanization, surface damage has become increasingly serious, leading to a series of environmental problems such as soil degradation, biodiversity loss, and soil erosion, while also threatening human sustainable development. To address this issue, the research and application of ecological restoration technologies have become particularly important. Traditional restoration methods typically involve covering the surface to be restored with a single remediation material and then planting vegetation; however, this method results in poor plant growth and ineffective restoration. Therefore, how to improve remediation materials to further enhance the ecological restoration effect has become a challenging problem in this field. Summary of the Invention
[0003] The purpose of this invention is to propose a steam-exploded cotton stalk microbial composite ecological restoration material and its production method. The continuous steam explosion equipment is used to achieve continuous and large-scale pretreatment of cotton stalks, which has high process efficiency and low energy consumption. At the same time, using agricultural waste cotton stalks as the base material, the raw materials are widely available and inexpensive, realizing the resource utilization of waste and conforming to the concept of circular economy.
[0004] The technical solution of this invention is implemented as follows: This invention provides a method for producing a steam-exploded cotton stalk microbial composite ecological restoration material. The method involves impregnating cotton stalks with acid or alkali solution and then steam-exploding them. The stalks are then added to a culture medium, inoculated with fermenting bacteria, and then NHS and EDC are added. The mixture is stirred and activated, and then nano-TiO2 modified with an aminosilane coupling agent is added. The mixture is stirred and reacted, filtered, washed, and dried to obtain the steam-exploded cotton stalk microbial composite ecological restoration material.
[0005] As a further improvement to the present invention, the following steps are included: S1. Steam explosion pretreatment: After the cotton stalks are soaked in acid or alkali solution and subjected to steam explosion treatment, they are washed and dried to obtain pretreated cotton stalks; S2. Preparation of modified nano-TiO2: Nano-TiO2 was added to ethanol, an amino-containing silane coupling agent was added, the mixture was heated and stirred to react, centrifuged, washed, and dried to obtain modified nano-TiO2; S3. Compound fermentation: Pretreated cotton stalks are added to a culture medium, inoculated with activated fermentation bacteria, fermented, then NHS and EDC are added, stirred and activated, modified nano TiO2 is added, stirred and reacted, filtered, washed, and dried to obtain steam-exploded cotton stalk microbial composite ecological restoration material.
[0006] As a further improvement of the present invention, the conditions for the steam explosion treatment in step S1 are 2-3 MPa, 200-250°C, and pressure maintained for 2-4 minutes followed by instantaneous pressure release.
[0007] As a further improvement of the present invention, the acid solution in step S1 is a hydrochloric acid solution with a concentration of 2-6 wt%, and the alkali solution is a NaOH followed by a KOH solution with a concentration of 5-10 wt%. The cotton stalks are first soaked in the acid or alkali solution, and then subjected to steam explosion treatment.
[0008] As a further improvement of the present invention, the mass ratio of nano-TiO2 and amino-containing silane coupling agent in step S2 is 100:3-5.
[0009] As a further improvement of the present invention, the heating and stirring reaction in step S2 is carried out at a temperature of 40-50°C for 2-4 hours.
[0010] As a further improvement of the present invention, the amino-containing silane coupling agent in step S2 is selected from at least one of KH550, KH602 or KH792.
[0011] As a further improvement of the present invention, the culture medium in step S3 is MRS culture medium, wherein the amount of pretreated cotton stalks added is 10-20 wt%, and the activated fermentation bacteria are a seed culture of Lactobacillus plantarum and Lactobacillus reuteri, with a bacterial count of 10. 8 -10 9 The inoculum concentration of the activated fermentation bacteria was 1-3 v / v% and 2-4 v / v%, respectively, and the fermentation conditions were 30-35℃, 100-200 r / min, and fermentation time was 18-24 h.
[0012] As a further improvement of the present invention, the mass ratio of NHS, EDC and modified nano-TiO2 in step S3 is 1-2:1-2:3-5, the stirring activation time is 15-30 min, and the stirring reaction time is 12-15 h.
[0013] This invention further protects a steam-exploded cotton stalk microbial composite ecological restoration material prepared by the above-mentioned production method.
[0014] The present invention has the following beneficial effects: Cotton stalks are agricultural waste, and traditional incineration causes serious environmental pollution. However, as an ecological restoration material, they possess advantages such as good compatibility, excellent mechanical properties, and biodegradability, making them a highly promising resource utilization pathway. Steam explosion technology primarily utilizes high-temperature, high-pressure steam to treat plant fiber raw materials, achieving component separation and structural changes through an instantaneous depressurization process.
[0015] Acid or alkali impregnation and steam explosion pretreatment effectively disrupt the dense woody fiber structure of cotton stalks, significantly increasing specific surface area and porosity, exposing more active sites such as hydroxyl groups, providing a foundation for subsequent grafting reactions; simultaneously, acid catalysis can partially hydrolyze hemicellulose, improving cellulose accessibility. The fiber structure after acid or alkali impregnation and steam explosion pretreatment has greater resistance to degradation, extending the material's service life.
[0016] The NHS / EDC coupling system achieves chemical bonding (amide bonds) between modified nano-TiO2 and proteases in the fermentation system, forming a stable three-dimensional network structure. This significantly improves the mechanical strength and structural stability of the material, preventing nanoparticle detachment. Simultaneously, the formation of an enzyme / TiO2 complex enhances synergistic catalysis, endowing the material with the ability to photocatalytically degrade organic pollutants. Microbial fermentation introduces functional bacterial communities, achieving a synergistic effect of photocatalysis and biodegradation, thus improving the remediation efficiency for complex polluted environments. The microbial fermentation process produces extracellular polymeric substances (EPS), plant hormones, and organic acids, which can improve the soil microecological environment and promote plant root growth. The chemically bonded nanoparticles and encapsulated microorganisms form a slow-release system, prolonging the reaction time and reducing the loss of active ingredients. Furthermore, aminosilane modification also endows TiO2 with good dispersibility and reactivity, enhancing its interfacial compatibility with the biomatrix.
[0017] The continuous steam explosion equipment enables continuous and large-scale pretreatment of cotton stalks, which is highly efficient and energy-saving. At the same time, using agricultural waste cotton stalks as the base material, the raw materials are widely available and inexpensive, realizing the resource utilization of waste and conforming to the concept of circular economy. Attached Figure Description
[0018] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0019] Figure 1 A photograph of the steam-exploded cotton stalk microbial composite ecological restoration material prepared in Example 1; Figure 2 This is a SEM image of the steam-exploded cotton stalk microbial composite ecological restoration material prepared in Example 1. Detailed Implementation
[0020] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0021] NHS, N-hydroxysuccinimide; EDC, 1-ethyl-(3-dimethylaminopropyl)carbodiimide. Example 1
[0022] This embodiment provides a method for producing a steam-exploded cotton stalk microbial composite ecological restoration material, including the following steps: S1. Steam explosion pretreatment: The cotton stalks are soaked in acid solution for 1 hour, steam explosion treatment is performed, and then washed and dried to obtain pretreated cotton stalks. The steam explosion treatment conditions are 2 MPa, 200°C, pressure maintained for 2 minutes and then instantaneously depressurized; the acid solution is a 2 wt% hydrochloric acid solution.
[0023] S2. Preparation of modified nano-TiO2: 10g of nano-TiO2 with an average particle size of 100-200nm was added to 200mL of ethanol, 0.3g of silane coupling agent KH550 was added, the mixture was heated to 40℃, stirred for 2h, centrifuged, washed and dried to obtain modified nano-TiO2. S3. Compound fermentation: Pretreated cotton stalks were added to MRS medium at a concentration of 10 wt%, and the inoculum concentration was 10. 8 -10 9 CFU / mL activated Lactobacillus plantarum and Lactobacillus reuteri seed cultures were inoculated at 1 v / v% and 2 v / v%, respectively, at 30℃ and 100 r / min for 18 h. Then, 1 g NHS and 1 g EDC were added, and the mixture was stirred and activated for 15 min. 3 g modified nano TiO2 was added, and the mixture was stirred and reacted for 12 h. The mixture was then filtered, washed, and dried to obtain the steam-exploded cotton stalk microbial composite ecological restoration material. Figure 1 The image shows a photograph of the steam-exploded cotton stalk microbial composite ecological restoration material prepared in Example 1. As can be seen from the image, it has a loose structure. Figure 2 This is a SEM image of the steam-exploded cotton stalk microbial composite ecological restoration material prepared in Example 1. As shown in the image, the nanospheres are fixedly loaded onto the fiber surface. Example 2
[0024] This embodiment provides a method for producing a steam-exploded cotton stalk microbial composite ecological restoration material, including the following steps: S1. Steam explosion pretreatment: The cotton stalks are soaked in alkaline solution for 1 hour, steam explosion treatment is performed, and then washed and dried to obtain pretreated cotton stalks. The steam explosion treatment conditions are 3 MPa, 250°C, pressure maintained for 4 minutes followed by instantaneous pressure release; the alkaline solution is a 10 wt% NaOH solution.
[0025] S2. Preparation of modified nano-TiO2: 10g of nano-TiO2 with an average particle size of 100-200nm was added to 200mL of ethanol, 0.5g of silane coupling agent KH602 was added, the mixture was heated to 50℃, stirred for 4h, centrifuged, washed, and dried to obtain modified nano-TiO2. S3. Compound fermentation: Pretreated cotton stalks were added to MRS medium at a concentration of 20 wt%, and the inoculum concentration was 10. 8 -10 9 CFU / mL activated Lactobacillus plantarum and Lactobacillus reuteri seed cultures were inoculated at 3v / v% and 4v / v%, respectively, at 35℃ and 200r / min for 24h. Then, 2g NHS and 2g EDC were added, and the mixture was stirred and activated for 30min. 5g modified nano-TiO2 was added, and the mixture was stirred and reacted for 15h. The mixture was then filtered, washed, and dried to obtain the steam-exploded cotton stalk microbial composite ecological restoration material. Example 3
[0026] This embodiment provides a method for producing a steam-exploded cotton stalk microbial composite ecological restoration material, including the following steps: S1. Steam explosion pretreatment: The cotton stalks are soaked in acid solution for 1 hour, steam explosion treatment is performed, and then washed and dried to obtain pretreated cotton stalks. The steam explosion treatment conditions are 2.5 MPa, 220°C, pressure maintained for 3 minutes and then instantaneously depressurized; the acid solution is a 4 wt% hydrochloric acid solution.
[0027] S2. Preparation of modified nano-TiO2: 10g of nano-TiO2 with an average particle size of 100-200nm was added to 200mL of ethanol, 0.4g of silane coupling agent KH792 was added, the mixture was heated to 45℃, stirred for 3h, centrifuged, washed, and dried to obtain modified nano-TiO2. S3. Compound fermentation: Add pretreated cotton stalks to MRS medium at a concentration of 15 wt%, and inoculate with a bacterial count of 10. 8 -10 9 CFU / mL activated Lactobacillus plantarum and Lactobacillus reuteri seed cultures were inoculated at 2 v / v% and 3 v / v%, respectively, at 32℃ and 150 r / min for 21 h. Then, 1.5 g NHS and 1.5 g EDC were added, and the mixture was stirred and activated for 22 min. 4 g modified nano TiO2 was added, and the mixture was stirred and reacted for 13 h. The mixture was then filtered, washed, and dried to obtain the steam-exploded cotton stalk microbial composite ecological restoration material.
[0028] Comparative Example 1 The difference from Example 3 is that the steam explosion treatment in step S1 was not performed.
[0029] Includes the following steps: S1. Pretreatment: After crushing the cotton stalks, soak them in a 4wt% hydrochloric acid solution for 1 hour, filter, wash, and dry to obtain pretreated cotton stalks; S2. Preparation of modified nano-TiO2: 10g of nano-TiO2 with an average particle size of 100-200nm was added to 200mL of ethanol, 0.4g of silane coupling agent KH792 was added, the mixture was heated to 45℃, stirred for 3h, centrifuged, washed, and dried to obtain modified nano-TiO2. S3. Compound fermentation: Add pretreated cotton stalks to MRS medium at a concentration of 15 wt%, and inoculate with a bacterial count of 10. 8 -10 9 CFU / mL activated Lactobacillus plantarum and Lactobacillus reuteri seed cultures were inoculated at 2 v / v% and 3 v / v%, respectively, at 32℃ and 150 r / min for 21 h. Then, 1.5 g NHS and 1.5 g EDC were added, and the mixture was stirred and activated for 22 min. 4 g modified nano TiO2 was added, and the mixture was stirred and reacted for 13 h. The mixture was then filtered, washed, and dried to obtain the steam-exploded cotton stalk microbial composite ecological restoration material.
[0030] Comparative Example 2 The difference from Example 3 is that the acid solution in step S1 is replaced by water.
[0031] Includes the following steps: S1. Steam explosion pretreatment: The cotton stalks are soaked in water for 1 hour, steam explosion treatment is performed, and then washed and dried to obtain pretreated cotton stalks. The conditions for the steam explosion treatment are 2.5 MPa, 220°C, and pressure maintained for 3 minutes followed by instantaneous pressure release.
[0032] S2. Preparation of modified nano-TiO2: 10g of nano-TiO2 with an average particle size of 100-200nm was added to 200mL of ethanol, 0.4g of silane coupling agent KH792 was added, the mixture was heated to 45℃, stirred for 3h, centrifuged, washed, and dried to obtain modified nano-TiO2. S3. Compound fermentation: Add pretreated cotton stalks to MRS medium at a concentration of 15 wt%, and inoculate with a bacterial count of 10. 8 -10 9CFU / mL activated Lactobacillus plantarum and Lactobacillus reuteri seed cultures were inoculated at 2 v / v% and 3 v / v%, respectively, at 32℃ and 150 r / min for 21 h. Then, 1.5 g NHS and 1.5 g EDC were added, and the mixture was stirred and activated for 22 min. 4 g modified nano TiO2 was added, and the mixture was stirred and reacted for 13 h. The mixture was then filtered, washed, and dried to obtain the steam-exploded cotton stalk microbial composite ecological restoration material.
[0033] Comparative Example 3 The difference from Example 3 is that step S2 was not performed.
[0034] Includes the following steps: S1. Steam explosion pretreatment: The cotton stalks are soaked in acid solution for 1 hour, steam explosion treatment is performed, and then washed and dried to obtain pretreated cotton stalks. The steam explosion treatment conditions are 2.5 MPa, 220°C, pressure maintained for 3 minutes and then instantaneously depressurized; the acid solution is a 4 wt% hydrochloric acid solution.
[0035] S2. Compound fermentation: Pretreated cotton stalks were added to MRS medium at a concentration of 15 wt%, and the inoculum concentration was 10. 8 -10 9 CFU / mL activated Lactobacillus plantarum and Lactobacillus reuteri seed cultures were inoculated at 2 v / v% and 3 v / v%, respectively, at 32℃ and 150 r / min for 21 h. Then, 1.5 g NHS and 1.5 g EDC were added, and the mixture was stirred and activated for 22 min. 4 g of nano-TiO2 with an average particle size of 100-200 nm was added, and the mixture was stirred and reacted for 13 h. The mixture was then filtered, washed, and dried to obtain the steam-exploded cotton stalk microbial composite ecological restoration material.
[0036] Comparative Example 4 The difference from Example 3 is that Lactobacillus plantarum seed solution was not inoculated in step S3.
[0037] Includes the following steps: S1. Steam explosion pretreatment: The cotton stalks are soaked in acid solution for 1 hour, steam explosion treatment is performed, and then washed and dried to obtain pretreated cotton stalks. The steam explosion treatment conditions are 2.5 MPa, 220°C, pressure maintained for 3 minutes and then instantaneously depressurized; the acid solution is a 4 wt% hydrochloric acid solution.
[0038] S2. Preparation of modified nano-TiO2: 10g of nano-TiO2 with an average particle size of 100-200nm was added to 200mL of ethanol, 0.4g of silane coupling agent KH792 was added, the mixture was heated to 45℃, stirred for 3h, centrifuged, washed, and dried to obtain modified nano-TiO2. S3. Compound fermentation: Add pretreated cotton stalks to MRS medium at a concentration of 15 wt%, and inoculate with a bacterial count of 10. 8 -10 9 The inoculum of activated Lactobacillus reuteri (cfu / mL) was inoculated at a rate of 5 v / v%, fermented at 32℃ and 150 r / min for 21 h, then 1.5 g NHS and 1.5 g EDC were added, and the mixture was stirred and activated for 22 min. 4 g modified nano TiO2 was added, and the mixture was stirred and reacted for 13 h. The mixture was then filtered, washed, and dried to obtain the steam-exploded cotton stalk microbial composite ecological restoration material.
[0039] Comparative Example 5 The difference from Example 3 is that no Lactobacillus reuteri seed culture was inoculated in step S3.
[0040] Includes the following steps: S1. Steam explosion pretreatment: The cotton stalks are soaked in acid solution for 1 hour, steam explosion treatment is performed, and then washed and dried to obtain pretreated cotton stalks. The steam explosion treatment conditions are 2.5 MPa, 220°C, pressure maintained for 3 minutes and then instantaneously depressurized; the acid solution is a 4 wt% hydrochloric acid solution.
[0041] S2. Preparation of modified nano-TiO2: 10g of nano-TiO2 with an average particle size of 100-200nm was added to 200mL of ethanol, 0.4g of silane coupling agent KH792 was added, the mixture was heated to 45℃, stirred for 3h, centrifuged, washed, and dried to obtain modified nano-TiO2. S3. Compound fermentation: Add pretreated cotton stalks to MRS medium at a concentration of 15 wt%, and inoculate with a bacterial count of 10. 8 -10 9 The seed culture of activated Lactobacillus plantarum (cfu / mL) was inoculated at a rate of 5 v / v%, fermented at 32℃ and 150 r / min for 21 h, then 1.5 g NHS and 1.5 g EDC were added, and the mixture was stirred and activated for 22 min. 4 g modified nano TiO2 was added, and the mixture was stirred and reacted for 13 h. The mixture was then filtered, washed, and dried to obtain the steam-exploded cotton stalk microbial composite ecological restoration material.
[0042] Comparative Example 6 The difference from Example 3 is that the fermentation process in step S3 was not performed.
[0043] Includes the following steps: S1. Steam explosion pretreatment: The cotton stalks are soaked in acid solution for 1 hour, steam explosion treatment is performed, and then washed and dried to obtain pretreated cotton stalks. The steam explosion treatment conditions are 2.5 MPa, 220°C, pressure maintained for 3 minutes and then instantaneously depressurized; the acid solution is a 4 wt% hydrochloric acid solution.
[0044] S2. Preparation of modified nano-TiO2: 10g of nano-TiO2 with an average particle size of 100-200nm was added to 200mL of ethanol, 0.4g of silane coupling agent KH792 was added, the mixture was heated to 45℃, stirred for 3h, centrifuged, washed, and dried to obtain modified nano-TiO2. S3. Mixing: Add the pretreated cotton stalks to water at a dosage of 15wt%, add 1.5g NHS and 1.5g EDC, stir and activate for 22min, add 4g modified nano TiO2, stir and react for 13h, filter, wash, and dry to obtain steam explosion cotton stalk microbial composite ecological restoration material.
[0045] Test Example 1 The steam-exploded cotton stalk microbial composite ecological restoration materials prepared in Examples 1-3 and Comparative Examples 1-6 were subjected to tensile property tests according to GB / T1040.2-2006, with a humidity of 55%, a temperature of 23℃, and a tensile speed of 100 mm / min. The results are shown in Table 1.
[0046] Table 1
[0047] As can be seen from the table above, the steam-exploded cotton stalk microbial composite ecological restoration materials prepared in Examples 1-3 of this invention have good mechanical properties.
[0048] Test Example 2 Soil used in the experiment: The soil samples were collected from farmland in Changji, Xinjiang. Before the experiment, the soil samples were air-dried and passed through a 40-mesh sieve to remove plant roots, small stones and other impurities.
[0049] Test subjects: Steam-exploded cotton stalk microbial composite ecological restoration materials of Examples 1-3 and Comparative Examples 1-6.
[0050] Experimental method: Each pot of sand weighed 1500g, and 2400g of steam-exploded cotton stalk microbial composite ecological restoration material was mixed into each pot. The control group was mixed with an equal amount of sand. After thorough mixing, various indicators were measured. The results are shown in Table 2.
[0051] Table 2
[0052] As shown in the table above, the steam-exploded cotton stalk microbial composite ecological restoration material prepared by this invention can significantly improve soil fertility and reduce soil heavy metal content.
[0053] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.
Claims
1. A method for producing a steam-exploded cotton stalk microbial composite ecological restoration material, characterized in that, After cotton stalks are soaked in acid or alkali solution and steam-exploded, they are added to a culture medium, inoculated with fermenting bacteria, and then NHS and EDC are added. After stirring and activation, nano-TiO2 modified with aminosilane coupling agent is added, stirred and reacted, filtered, washed and dried to obtain steam-exploded cotton stalk microbial composite ecological restoration material.
2. The production method according to claim 1, characterized in that, Includes the following steps: S1. Steam explosion pretreatment: After the cotton stalks are soaked in acid or alkali solution and subjected to steam explosion treatment, they are washed and dried to obtain pretreated cotton stalks; S2. Preparation of modified nano-TiO2: Nano-TiO2 was added to ethanol, an amino-containing silane coupling agent was added, the mixture was heated and stirred to react, centrifuged, washed, and dried to obtain modified nano-TiO2; S3. Compound fermentation: Pretreated cotton stalks are added to a culture medium, inoculated with activated fermentation bacteria, fermented, then NHS and EDC are added, stirred and activated, modified nano TiO2 is added, stirred and reacted, filtered, washed, and dried to obtain steam-exploded cotton stalk microbial composite ecological restoration material.
3. The production method according to claim 2, characterized in that, The conditions for steam explosion treatment in step S1 are 2-3 MPa, 200-250°C, and pressure maintained for 2-4 minutes followed by instantaneous pressure release.
4. The production method according to claim 2, characterized in that, The acid solution mentioned in step S1 is a hydrochloric acid solution with a concentration of 2-6 wt%, and the alkaline solution is a NaOH followed by KOH solution with a concentration of 5-10 wt%.
5. The production method according to claim 2, characterized in that, In step S2, the mass ratio of nano-TiO2 to amino-containing silane coupling agent is 100:3-5.
6. The production method according to claim 2, characterized in that, The heating and stirring reaction in step S2 is carried out at a temperature of 40-50°C for 2-4 hours.
7. The production method according to claim 2, characterized in that, The amino-containing silane coupling agent mentioned in step S2 is selected from at least one of KH550, KH602 or KH792.
8. The production method according to claim 2, characterized in that, The culture medium mentioned in step S3 is MRS medium, wherein the amount of pretreated cotton stalks added is 10-20 wt%, and the activated fermentation bacteria are a seed culture of Lactobacillus plantarum and Lactobacillus reuteri, with a bacterial count of 10. 8 -10 9 The inoculum concentration of the activated fermentation bacteria was 1-3 v / v% and 2-4 v / v%, respectively, and the fermentation conditions were 30-35℃, 100-200 r / min, and fermentation time was 18-24 h.
9. The production method according to claim 2, characterized in that, In step S3, the mass ratio of NHS, EDC and modified nano-TiO2 is 1-2:1-2:3-5, the stirring activation time is 15-30 min, and the stirring reaction time is 12-15 h.
10. Steam-exploded cotton stalk microbial composite ecological restoration material prepared by the production method according to any one of claims 1-9.