Microbial organic fertilizer and application thereof
By combining microbial organic fertilizers to improve the rhizosphere microecology, soil and disease problems caused by chemical fertilizers have been solved, resulting in improved crop yield and quality.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- YUNAN JIJIFENG AGRI MATERIALS CHAIN
- Filing Date
- 2026-03-20
- Publication Date
- 2026-05-01
AI Technical Summary
The long-term excessive application of chemical fertilizers leads to soil compaction, imbalance of microbial community structure, aggravation of soil-borne diseases, and increased risk of nitrate and heavy metal residues in agricultural products, affecting sustainable agricultural development and food safety.
This microbial organic fertilizer uses a combination of organic components, including soybean peptide powder, enzymatic hydrolysate, mineral humic acid, and mushroom residue powder, along with microbial agents such as Japanese slow-growing rhizobium, Micrococcus luteus, and Bacillus subtilis. By improving the rhizosphere microecological environment, it can prevent and control clubroot disease in Chinese cabbage, bacterial wilt in tomatoes, and root rot in tea trees.
It effectively prevents and controls clubroot disease in Chinese cabbage, bacterial wilt in tomatoes, and root rot in tea trees, thereby increasing crop yield and quality, improving soil health, and reducing environmental pollution.
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Abstract
Description
A microbial organic fertilizer and its application Technical Field
[0001] This invention belongs to the field of biotechnology, specifically, it relates to a microbial organic fertilizer and its application. Background Technology
[0002] With the rapid development of modern agriculture, the long-term and excessive application of chemical fertilizers and pesticides has triggered a series of serious ecological and environmental problems and food safety challenges. These problems mainly include soil compaction, decline in organic matter, imbalance of microbial community structure, aggravation of soil-borne diseases, and increased risk of nitrate and heavy metal residues in agricultural products. This not only threatens the sustainable development of agriculture but also poses a potential threat to human health. Therefore, developing new green agricultural inputs that are environmentally friendly and can improve soil health and agricultural product quality has become an important direction for global agricultural technology.
[0003] Microbial fertilizers, as living preparations, utilize the life activities of highly active beneficial microorganisms (such as nitrogen-fixing bacteria, phosphate-solubilizing bacteria, potassium-solubilizing bacteria, growth-promoting bacteria, and biocontrol bacteria) in the soil. These microorganisms transform nutrients that are difficult for crops to absorb into available forms, secrete plant growth regulators, inhibit soil-borne pathogens, and improve the rhizosphere microecological environment. Compared to traditional chemical fertilizers, microbial fertilizers offer multiple advantages, including soil improvement, increased fertilizer utilization, enhanced crop resistance, improved crop growth, improved crop quality, and reduced environmental pollution. They are a key technology for achieving "reduced fertilizer use and increased efficiency" and green production in agriculture.
[0004] Chinese cabbage, tomatoes, and tea are important economic crops in most parts of Yunnan. Clubroot disease in Chinese cabbage affects its yield, bacterial wilt in tomatoes severely impacts yield, and root rot in tea trees leads to reduced yield, lower tea quality, and even tree death. Based on these issues, the applicant has developed a microbial organic fertilizer. Summary of the Invention
[0005] In order to overcome the problems existing in the background art, the present invention provides a microbial organic fertilizer and its application, which can effectively prevent clubroot disease of Chinese cabbage, bacterial wilt of tomatoes and root rot of tea trees.
[0006] To achieve the above objectives, the first aspect of the present invention provides a microbial organic fertilizer comprising the following components: Component A, comprising the following components in parts by mass: 4-6 parts soybean peptide powder, 18-22 parts soybean enzymatic hydrolysate, 18-24 parts corn cob furfural residue powder, 14-16 parts mineral humic acid, 12-18 parts mushroom residue powder, 6-15 parts rice husk powder, 6-9 parts oyster shell powder, and 5-7 parts bovine bone powder; Component B, comprising Bradyrhizobium japonicum inoculant, Micrococcus luteus inoculant, and Bacillus subtilis inoculant in a mass ratio of 1:1-2:3-6; wherein the mass of Component B is 1%-3% of Component A.
[0007] Furthermore, the effective viable count of both the Japanese slow-growing rhizobium inoculant and the Garcinia cambogia inoculant is not less than 1×10⁻⁶. 7 CFU / g, the number of viable bacteria in Bacillus subtilis inoculant is not less than 1×10⁻⁶. 8 CFU / g.
[0008] Furthermore, the preparation method of the *Staphylococcus aureus* inoculant is as follows: 5% liquid inoculum is inoculated into a liquid fermentation medium of *Staphylococcus aureus*, and cultured aerobically at 28-30℃, 180-220 r / min for 36-48 h. After filtering and drying, a mixture of trehalose, skim milk powder, and diatomaceous earth in a mass ratio of 1:1:1 is added to achieve an effective viable count of 1×10⁻⁶ *Staphylococcus aureus* inoculant. 7 CFU / g; The liquid fermentation medium of Japanese slow-growing rhizobium contains: yeast extract 3.0g / L, glucose 10.0g / L, tryptone 5.0g / L, NaCl 0.5g / L, MgSO4·7H2O 0.5g / L, pH value: 6.8-7.2.
[0009] Further, the preparation method of the *Micrococcus luteus* inoculant is as follows: 3% liquid inoculum is inoculated into a liquid fermentation medium of *Micrococcus luteus*, and cultured aerobicly at 26-30℃, 180-200 r / min for 24-36 h. After filtration and drying, a mixture of trehalose, skim milk powder, and diatomaceous earth in a mass ratio of 1:1:1 is added to achieve an effective viable count of 1×10⁻⁶ *Micrococcus luteus* inoculant. 7 CFU / g; wherein, the liquid fermentation medium of Micrococcus luteus contains: 10.0 g / L peptone, 3.0 g / L beef meal, 5.0 g / L sodium chloride, and pH value: 7.3±0.1.
[0010] Furthermore, the preparation method of the Bacillus subtilis inoculant is as follows: 3% liquid inoculum is inoculated into a Bacillus subtilis liquid fermentation medium, and cultured aerobicly at 28-32℃, 180-220 r / min for 36-48 h. After filtering and drying, a mixture of trehalose, skim milk powder, and diatomaceous earth in a mass ratio of 1:1:1 is added to achieve an effective viable count of 1×10⁻⁶ Bacillus subtilis inoculant. 8 CFU / g; wherein, the Bacillus subtilis liquid fermentation medium contains: peptone 10.0 g / L, beef meal 3.0 g / L, sodium chloride 5.0 g / L, pH value: 7.3±0.1.
[0011] The second aspect of this invention provides the application of the microbial organic fertilizer of the first aspect in the prevention and control of bacterial wilt in tomatoes and the increase of tomato yield.
[0012] The third aspect of this invention provides the application of the microbial organic fertilizer of the first aspect in the prevention and control of clubroot disease in Chinese cabbage and the increase of Chinese cabbage yield.
[0013] The fourth aspect of this invention provides the application of the microbial organic fertilizer of the first aspect in preventing and controlling root rot in tea trees and increasing tea yield.
[0014] The beneficial effects of the present invention are as follows: 1. The microbial organic fertilizer provided by the present invention can effectively prevent and control bacterial wilt caused by Pseudomonas aeruginosa and increase the yield of tomatoes.
[0015] 2. The microbial organic fertilizer provided by this invention can effectively prevent and control clubroot disease in Chinese cabbage caused by Plasmodiophora abrassicae, and increase the yield of Chinese cabbage.
[0016] 3. The microbial organic fertilizer provided by this invention can effectively prevent and control root rot of tea trees caused by Rosellia arcuata. Detailed Implementation
[0017] To make the objectives, technical solutions, and beneficial effects of the present invention clearer, the preferred embodiments of the present invention will be described in detail below to facilitate understanding by those skilled in the art.
[0018] Soybean peptide powder was purchased from Shandong Pingju Biotechnology Co., Ltd.; mineral humic acid was purchased from Shandong Yishunfa Chemical Co., Ltd.; rice husk powder was purchased from Rongze Agriculture (Shijiazhuang) Co., Ltd.; oyster shell powder was purchased from Lingshou County Defa Mineral Products Processing Plant; and bovine bone powder was purchased from Jinan Yucai Chemical Co., Ltd. Japanese slow-growing rhizobium was purchased from Shangcheng Beina Chuanglian Biotechnology Co., Ltd., product number: BNCC224684; Micrococcus luteus was purchased from Shangcheng Beina Chuanglian Biotechnology Co., Ltd., product number: BNCC195416; and Bacillus subtilis was purchased from Shangcheng Beina Chuanglian Biotechnology Co., Ltd., product number: BNCC188080. The preparation method of soybean enzymatic hydrolysate was as follows: soybeans were pulverized, and hydrolyzed with 0.05wt% alkaline protease at pH=8.5 and 50℃ for 10 hours. After enzyme inactivation, the solution was filtered and concentrated to a density of 1.20 g / cm³. 3 This yields soybean enzymatic hydrolysate.
[0019] The preparation method of *Staphylococcus aureus* inoculant is as follows: 5% liquid inoculum is inoculated into a liquid fermentation medium containing *Staphylococcus aureus*. The mixture is then cultured aerobically at 28-30℃, 180-220 r / min for 36-48 hours. After filtration and drying, a mixture of trehalose, skim milk powder, and diatomaceous earth in a 1:1:1 mass ratio is added to achieve an effective viable count of 1×10⁻⁶ bacteria in the *Staphylococcus aureus* inoculant. 7 CFU / g; The liquid fermentation medium of Japanese slow-growing rhizobium contains: yeast extract 3.0g / L, glucose 10.0g / L, tryptone 5.0g / L, NaCl 0.5g / L, MgSO4·7H2O 0.5g / L, pH value: 6.8-7.2.
[0020] The preparation method of *Micrococcus luteus* inoculant is as follows: 3% liquid inoculum is inoculated into *Micrococcus luteus* liquid fermentation medium, and cultured aerobicly at 26-30℃, 180-200 r / min for 24-36 h. After filtration and drying, a mixture of trehalose, skim milk powder, and diatomaceous earth in a mass ratio of 1:1:1 is added to achieve an effective viable count of 1×10⁻⁶ *Micrococcus luteus* inoculant. 7 CFU / g; wherein, the liquid fermentation medium of Micrococcus luteus contains: 10.0 g / L peptone, 3.0 g / L beef meal, 5.0 g / L sodium chloride, and pH value: 7.3±0.1.
[0021] The preparation method of Bacillus subtilis inoculant is as follows: 3% liquid inoculum is inoculated into Bacillus subtilis liquid fermentation medium, and cultured aerobicly at 28-32℃, 180-220 r / min for 36-48 h. After filtration and drying, a mixture of trehalose, skim milk powder, and diatomaceous earth in a mass ratio of 1:1:1 is added to achieve an effective viable count of 1×10⁻⁶ Bacillus subtilis inoculant. 8CFU / g; wherein, the Bacillus subtilis liquid fermentation medium contains: peptone 10.0 g / L, beef meal 3.0 g / L, sodium chloride 5.0 g / L, pH value: 7.3±0.1.
[0022] Preparation method of well-rotted sheep manure: Add sawdust to sheep manure to adjust the C / N ratio to 25:1, add EM bacteria, add water and stir until the moisture content is 50%-60%, then build a pile for fermentation; maintain: heating stage (1-3 days): the temperature inside the pile rises rapidly to above 55℃, the high temperature stage is 55-65℃ for 10 days, when the pile temperature exceeds 70℃ or begins to drop, turn the pile. Turn the outer layer of material to the inside, and the inside to the outside, to add oxygen and make fermentation uniform. Check the moisture content when turning the pile, and add water if it becomes dry. The temperature gradually drops to room temperature, the material color turns dark brown or blackish brown, and there is no odor. At this time, pile it tightly and compact it, cover it with straw and let it stand for further maturation for 1 month, then spread it out to dry until the moisture content is about 35%, which is the well-rotted sheep manure.
[0023] Example 1: A microbial organic fertilizer comprising the following components: Component A, comprising the following components in parts by mass: 5 parts soybean peptide powder, 20 parts soybean enzymatic hydrolysate, 22 parts corn cob furfural residue powder, 15 parts mineral humic acid, 15 parts mushroom residue powder (obtained by crushing king oyster mushroom residue), 11 parts rice husk powder, 7 parts oyster shell powder, and 6 parts bovine bone powder; Component B, comprising Bradyrhizobium japonicum inoculant, Micrococcus luteus inoculant, and Bacillus subtilis inoculant in a mass ratio of 1:1.5:4.5; the mass of Component B is 1.5% of Component A.
[0024] Example 2: A microbial organic fertilizer comprising the following components: Component A, comprising the following components in parts by mass: 4 parts soybean peptide powder, 18 parts soybean enzymatic hydrolysate, 18 parts corn cob furfural residue powder, 14 parts mineral humic acid, 12 parts mushroom residue powder (obtained by crushing king oyster mushroom residue), 6 parts rice husk powder, 6 parts oyster shell powder, and 5 parts bovine bone powder; Component B, comprising Bradyrhizobium japonicum inoculant, Micrococcus luteus inoculant, and Bacillus subtilis inoculant in a mass ratio of 1:1:3; the mass of Component B is 1% of Component A.
[0025] Example 3: A microbial organic fertilizer comprising the following components: Component A, comprising the following components in parts by mass: 6 parts soybean peptide powder, 22 parts soybean enzymatic hydrolysate, 24 parts corn cob furfural residue powder, 16 parts mineral humic acid, 18 parts mushroom residue powder (obtained by crushing king oyster mushroom residue), 15 parts rice husk powder, 9 parts oyster shell powder, and 7 parts bovine bone powder; Component B, comprising Bradyrhizobium japonicum inoculant, Micrococcus luteus inoculant, and Bacillus subtilis inoculant in a mass ratio of 1:2:6; the mass of Component B is 3% of Component A.
[0026] The difference between Comparative Example 1 and Example 1 is that the microbial organic fertilizer does not contain Japanese slow-growing rhizobium inoculant.
[0027] The difference between Comparative Example 2 and Example 1 is that the microbial organic fertilizer does not contain Micrococcus luteus inoculant.
[0028] The difference between Comparative Example 3 and Example 1 is that the microbial organic fertilizer does not contain Bacillus subtilis inoculant.
[0029] Experiment 1: Potted Plant Experiment 1. Cabbage Potted Plant Experiment Select healthy cabbage seedlings without obvious pests and diseases and transplant them into flower pots. Transplant one seedling into each pot. After they have fully survived, select the potted seedlings that are growing well after transplanting and without obvious pests and diseases. 100 pots for each treatment (Examples 1 to 3, Comparative Examples 1 to 3 and Positive Control), with three replicates for each treatment.
[0030] Around the roots of each treatment of potted cabbage seedlings, a ring of soil was dug, and 300g of the microbial organic fertilizer prepared for each treatment was applied to each seedling. After covering with soil and watering, the seedlings were cultivated under normal management for 15 days. Then, the roots of the potted cabbage seedlings were drenched with Plasmodiophora abrassicae bacterial suspension. The positive control was not treated with microbial organic fertilizer, but was watered and drenched with Plasmodiophora abrassicae bacterial suspension.
[0031] For each potted Chinese cabbage seedling, drench the roots with 200mL of Plasmodiophora brassicae bacterial suspension, with an effective viable count of 1×10⁻⁶ bacteria. 8 CFU / mL.
[0032] The rest were managed normally. The disease incidence rate of the cabbage plants was investigated after 14 days. The disease incidence rate was calculated as (number of diseased plants / total number of plants investigated) * 100%. The statistical results are shown in Table 1 below.
[0033] Table 1. Statistics on Disease Incidence in Chinese Cabbage Plants 2. Tomato pot experiment: Select tomato seedlings with good growth and no obvious diseases and pests and transplant them into flower pots. Transplant one seedling into each pot. After they have fully survived, select potted seedlings with strong growth and no obvious diseases and pests after transplanting. 100 pots for each treatment (Examples 1 to 3, Comparative Examples 1 to 3 and Positive Control), and three replicates for each treatment.
[0034] A ring of soil was dug around the roots of each treatment of tomato potted seedlings, and 500g of the microbial organic fertilizer prepared for each treatment was applied to each seedling. After covering with soil and watering, the seedlings were cultivated under normal management for 15 days. Then, the roots of the tomato potted seedlings were drenched with a suspension of Pseudomonas solanacearum. The positive control was not treated with microbial organic fertilizer, but was watered and drenched with a suspension of Pseudomonas solanacearum.
[0035] Drench each potted tomato seedling with 200 mL of Pseudomonas solanacearum bacterial suspension, with an effective viable count of 1 × 10⁻⁶ bacteria. 8 CFU / mL.
[0036] The rest were managed normally. The disease incidence rate of tomato plants was investigated after 14 days. The disease incidence rate was calculated as (number of diseased plants / total number of plants investigated) * 100%. The statistical results are shown in Table 2 below.
[0037] Table 2. Statistics on Disease Incidence in Tomato Plants 3. Tea tree pot experiment: Select tea tree seedlings with a height of about 15cm and a diameter of 0.2cm from the nursery and transplant them into flower pots. Transplant one seedling into each pot. After they have fully survived, select potted seedlings that are growing well, have green leaves and no obvious diseases or pests. 50 pots for each treatment (Examples 1 to 3, Comparative Examples 1 to 3 and Positive Control), with three replicates for each treatment.
[0038] Around the roots of each treatment potted tea seedling, a ring of soil was dug, and 2.5 kg of the microbial organic fertilizer prepared for each treatment was applied to each seedling. After covering with soil and watering, the seedlings were cultivated under normal management for 15 days. Then, the roots of the potted tea seedlings were drenched with a suspension of Rosellia arcuata. The positive control was not treated with microbial organic fertilizer, but was watered and drenched with Rosellia arcuata suspension.
[0039] Each potted tea seedling should be irrigated with 200 mL of Rosellia arcuata suspension, with an effective viable count of 1 × 10⁻⁶ bacteria. 8 CFU / mL.
[0040] The rest were managed normally. The disease incidence rate of the tea plants was investigated after 30 days. The disease incidence rate was calculated as (number of diseased plants / total number of plants investigated) * 100%. The statistical results are shown in Table 3 below.
[0041] Table 3. Statistics on Disease Incidence in Tea Plants II. Field Experiment 1. Land Preparation for the Chinese Cabbage Field Planting Experiment: Seven 1-acre greenhouse planting plots were selected. Six of these plots were designated as six treatments, and microbial organic fertilizers from Examples 1-3 and Comparative Examples 1-3 were applied as base fertilizers at a rate of 250 kg per acre. The remaining plot served as a control group, and 1500 kg of fermented and decomposed sheep manure + 40 kg of compound fertilizer (17-17-17) were applied as base fertilizers per acre.
[0042] Transplanting: Select robust cabbage seedlings without obvious diseases or pests and transplant them to the greenhouse planting plots after land preparation, with 3,500 seedlings per acre.
[0043] Management: For each treatment, 10 days after transplanting, apply 8 kg of compound fertilizer (20-10-10) per mu, diluted with 1000 kg of water, and apply it in holes next to the plants; 15 days after the last fertilization, apply 12 kg of compound fertilizer (20-10-10) per mu, diluted with 1200 kg of water, and apply it in holes next to the plants; during the heading stage of Chinese cabbage, apply 20 kg of urea and 10 kg of potassium sulfate per mu for each treatment, and manage normally for the rest.
[0044] After harvesting the cabbage, peel off the outer outer leaves and weigh them.
[0045] Table 4. Statistics on Chinese cabbage production 2. Tomato field planting experiment land preparation: Seven 1-acre greenhouse planting plots were selected. Six of these plots were designated as six treatments, and microbial organic fertilizers from Examples 1-3 and Comparative Examples 1-3 were applied as base fertilizers at a rate of 300 kg per acre. The remaining plot served as a control group, and 2000 kg of fermented and decomposed sheep manure + 40 kg of compound fertilizer (17-17-17) + 50 kg of superphosphate + 12 kg of potassium sulfate were applied as base fertilizers per acre.
[0046] Transplanting: Select healthy tomato seedlings without obvious diseases and pests and transplant them to the greenhouse planting plots after land preparation, with 2,000 seedlings per acre.
[0047] Management: Water once 7 days after transplanting for each treatment; do not water until the soil is dry before the first cluster of flowers sets fruit; when the first cluster of fruit swells to the size of a ping-pong ball, apply the first heavy topdressing. Apply 20 kg of high-potassium compound fertilizer (15-5-25) + 8 kg of urea per acre with irrigation water. Afterward, keep the soil moist, avoiding sudden changes in moisture to prevent fruit cracking. Apply topdressing once after every two clusters of fruit harvested, applying 10 kg of potassium sulfate + 5 kg of calcium ammonium nitrate per acre each time, and manage normally for the rest of the time.
[0048] The weight of tomatoes harvested each time was counted, and the yield of each treatment was combined at the end of the last crop. The results are shown in Table 5 below.
[0049] Table 5. Tomato Yield Statistics 3. Field Experiment of Tea Plantations: The test site was a tea plantation in Mile City, Yunnan Province. The soil physicochemical properties were as follows: lateritic red soil, available phosphorus 7.9 mg / kg, organic matter 97.33 g / kg, moisture content 11.02%, pH 5.02.
[0050] Experimental crop: Tieguanyin tea, 12-year-old trees. Experimental method: A randomized block design was used, with 7 treatments, each with a 20m² area. 2 The experiment was repeated three times. Six treatments were applied with the microbial organic fertilizers prepared in Examples 1 to 3 and Comparative Examples 1 to 3, respectively, at a rate of 60 kg per mu (approximately 0.067 hectares). These treatments were labeled as Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, and Comparative Example 3. The seventh treatment was a compound fertilizer (N:P:K = 17:17:17), which was designated as the control group. The fertilization method was as follows: base fertilizer was applied in mid-December and topdressing was applied in mid-June using a strip-ditch fertilization method. The control group received 30 kg of fertilizer per mu each time. Other management methods were the same as local conventional management methods.
[0051] In early March of the following year, the physical and chemical properties of the soil were tested. Soil samples were collected in accordance with the "Technical Specifications for Soil Analysis". The pH value was determined using a CHN-82801 pH meter (water-soil ratio 2.5:1). The organic matter content was determined using the potassium dichromate heating method. The available phosphorus content was determined using the sodium hydroxide fusion-molybdenum antimony colorimetric method. The results are shown in Table 6.
[0052] Table 6. Statistical table of soil physicochemical properties When harvesting tea at the end of March of the second year, one bud and one leaf were picked. After calculating the fresh weight of the tea leaves (yield, kg / mu), the tea leaves were first dried at 120℃ for 5 minutes in a dryer, and then dried at 80℃ to constant weight. The contents of free amino acids, tea polyphenols and caffeine in the tea leaves dried to constant weight were calculated (the detection methods refer to GB / T 8313—2018 and GB / T 8314—2013). The results are shown in Table 7.
[0053] Table 7. Statistics on Tea Yield and Tea Component Content As can be seen from Tables 1-7, the bio-organic fertilizer provided in this application can effectively prevent and control clubroot disease caused by Plasmodiophora abrassicae and increase the yield of Chinese cabbage; the bio-organic fertilizer provided in this application can effectively prevent and control bacterial wilt of tomatoes caused by Pseudomonas solanacearum and increase the yield of tomatoes; the bio-organic fertilizer provided in this application can effectively prevent and control root rot of tea trees caused by Rosellia arcuata and increase the yield and quality of tea.
[0054] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made to it in form and detail without departing from the scope defined by the claims of the present invention.
Claims
1. A microbial organic fertilizer, characterized in that: The product comprises the following components: Component A, which contains the following components in parts by weight: 4-6 parts soybean peptide powder, 18-22 parts soybean enzymatic hydrolysate, 18-24 parts corn cob furfural residue powder, 14-16 parts mineral humic acid, 12-18 parts mushroom residue powder, 6-15 parts rice husk powder, 6-9 parts oyster shell powder, and 5-7 parts bovine bone powder; Component B, which contains Bradyrhizobium japonicum, Micrococcus luteus, and Bacillus subtilis inoculants in a mass ratio of 1:1-2:3-6; the mass of Component B is 1%-3% of that of Component A.
2. The microbial organic fertilizer according to claim 1, characterized in that: The effective viable bacteria count in the Japanese slow-growing rhizobium inoculant and the Garcinia cambogia micrococcus inoculant is not less than 1×10⁻⁶. 7 CFU / g, the number of viable bacteria in Bacillus subtilis inoculant is not less than 1×10⁻⁶. 8 CFU / g.
3. The microbial organic fertilizer according to claim 1, characterized in that: The preparation method of the Japanese slow-growing rhizobium inoculant is as follows: 5% liquid inoculum is inoculated into a liquid fermentation medium of Japanese slow-growing rhizobium, and cultured aerobicly at 28-30℃, 180-220 r / min for 36-48 h. After filtration and drying, a mixture of trehalose, skim milk powder, and diatomaceous earth in a mass ratio of 1:1:1 is added to achieve an effective viable count of 1×10⁻⁶ bacteria in the Japanese slow-growing rhizobium inoculant. 7 CFU / g; the liquid fermentation medium of Japanese slow-growing rhizobium contains: yeast extract 3.0g / L, glucose 10.0g / L, tryptone 5.0g / L, NaCl 0.5g / L, MgSO4·7H2O 0.5g / L, pH value: 6.8-7.
2.
4. The microbial organic fertilizer according to claim 1, characterized in that: The preparation method of the *Micrococcus luteus* inoculant is as follows: 3% liquid inoculum is inoculated into a liquid fermentation medium of *Micrococcus luteus*, and cultured aerobicly at 26-30℃, 180-200 r / min for 24-36 h. After filtration and drying, a mixture of trehalose, skim milk powder, and diatomaceous earth in a mass ratio of 1:1:1 is added to achieve an effective viable count of 1×10⁻⁶ *Micrococcus luteus* inoculant. 7 CFU / g; wherein, the liquid fermentation medium of Micrococcus luteus contains: 10.0 g / L peptone, 3.0 g / L beef meal, 5.0 g / L sodium chloride, and pH value: 7.3±0.
1.
5. The microbial organic fertilizer according to claim 1, characterized in that: The preparation method of the Bacillus subtilis inoculant is as follows: 3% liquid inoculum is inoculated into a Bacillus subtilis liquid fermentation medium, and cultured aerobicly at 28-32℃, 180-220 r / min for 36-48 h. After filtration and drying, a mixture of trehalose, skim milk powder, and diatomaceous earth in a mass ratio of 1:1:1 is added to achieve an effective viable count of 1×10⁻⁶ Bacillus subtilis inoculant. 8 CFU / g; wherein, the Bacillus subtilis liquid fermentation medium contains: peptone 10.0 g / L, beef meal 3.0 g / L, sodium chloride 5.0 g / L, pH value: 7.3±0.
1.
6. The application of the microbial organic fertilizer as described in any one of claims 1-5 in the prevention and control of bacterial wilt in tomatoes and the increase of tomato yield.
7. The application of the microbial organic fertilizer as described in any one of claims 1-5 in the prevention and control of clubroot disease in Chinese cabbage and the increase of Chinese cabbage yield.
8. The application of the microbial organic fertilizer as described in any one of claims 1-5 in preventing and controlling root rot in tea trees and increasing tea yield.