Application of cothane dimethyl ether and sakuranetin in prevention and treatment of wheat scab

Abstract

The invention discloses an application of cothine dimethyl ether and sakuranetin as a bacteriostatic agent of a plant pathogen fusarium graminearum, and an application of the cothine dimethyl ether and the sakuranetin in prevention and treatment of wheat scab. According to the present invention, the prevention and the control on the wheat scab are achieved by inhibiting the mycelial growth of fusarium graminearum, inhibiting the biosynthesis of deoxynivalenol, and / or inhibiting the expression of the deoxynivalenol synthesis key gene. Both the cocaine dimethyl ether and the sakuranetin are plant source natural products and have the characteristics of low toxicity, easiness in degradation, high environmental compatibility and the like. Experimental data provided by the invention fully prove that the compound is effective in inhibiting growth of fusarium graminearum and accumulation of DON, is safe and harmless to wheat, can be used as a green replacement or supplement means of a chemical bactericide, provides a new solution for green prevention and control of wheat scab, and has important application value and popularization prospect.

Description

Technical Field

[0001] This invention relates to the field of biotechnology, and in particular to the application of chloroquine dimethyl ether and sakurain in the control of wheat scab. Background Technology

[0002] Wheat head blight (FHB) is a significant disease in wheat production. Due to low resistance levels in wheat and the scarcity of resistant varieties, this disease typically causes yield losses exceeding 10%, and in severe cases, reductions can reach 50%. The primary pathogen causing wheat head blight is *Fusarium graminearum*. Fusarium graminearum It easily infects the ears of wheat during the flowering stage, which not only significantly reduces yield, but also produces a variety of fungal toxins during the infection period, the most important of which is deoxynivalenol (DON).

[0003] Existing research has shown that DON is a virulence factor that can accelerate Fusarium graminearum infection in wheat and promote the spread of Fusarium head blight. Furthermore, excessive consumption of DON-contaminated grains by humans and livestock can cause symptoms such as nausea, vomiting, diarrhea, and abdominal pain, and may even damage the immune system and lead to cell carcinogenesis. In addition, DON maintains high stability under weakly acidic, high-temperature, and high-pressure conditions, making the removal of this toxin from contaminated products extremely difficult. Due to its high toxicity and stability, DON has been listed by the FAO and WHO as one of the most dangerous natural food contaminants. Therefore, developing effective strategies to mitigate DON contamination in wheat has become a key task for ensuring food safety and sustainable agricultural development.

[0004] Fungicides can effectively control Fusarium head blight infection, thereby indirectly reducing the DON content in wheat ears. However, numerous in vitro experiments have shown that while some fungicides can effectively inhibit Fusarium head blight growth, when the concentration is reduced to sublethal levels, they often fail to reduce—and may even exacerbate—DON accumulation. For example, sublethal concentrations of methoxyacrylate fungicides, such as azoxystrobin, tebuconazole, and fluopyram, can increase acetyl-CoA levels and upregulate key genes in DON biosynthesis. Tri5 and Tri6 This promotes DON production. Similarly, sublethal concentrations of triazole fungicides (such as tebuconazole, flutriafol, propiconazole, and thiophanate-methyl) can inhibit sterol biosynthesis by inhibiting sterol 14α-demethylase, leading to lanosterol accumulation and the use of the common precursor farnesyl pyrophosphate in DON synthesis, thereby increasing DON production. Furthermore, residual chemical fungicides may accumulate in the environment or organisms, causing pollution. Therefore, it is crucial to explore other substances with lower toxicity that can inhibit the growth or toxin production of Fusarium graminearum.

[0005] Plants and their metabolites have become a hot topic in green pesticide research in recent years due to their natural origin, high environmental compatibility, and diverse mechanisms of action. However, few natural metabolites with inhibitory activity against Fusarium graminearum growth or DON synthesis have been systematically identified and applied. Chrysin dimethyl ether and Sakuranetin are two flavonoids found in plants and are known to have certain antioxidant and anti-inflammatory biological activities. However, to date, there are no research reports on the use of chrysin dimethyl ether or Sakuranetin for the control of wheat scab, inhibition of Fusarium graminearum growth, or reduction of DON toxin accumulation.

[0006] Therefore, exploring the application potential of these two substances in the control of Fusarium graminearum is of great scientific significance and application value for the development of new green pesticides. Summary of the Invention

[0007] The present invention aims to solve the above-mentioned problems. In a first aspect, the present invention provides ketone dimethyl ether as a treatment for the plant pathogen *Fusarium graminearum* (…). Fusarium graminearum Uses of antibacterial agents. Chrysindimethyl ether, belonging to the flavonoid class, has the molecular formula C64. 17 H 14 O4 has a relative molecular mass of 282.10.

[0008] Secondly, this invention provides cherry blossom extract as a plant pathogen of Fusarium graminearum (… Fusarium graminearum Uses of antibacterial agents. Sakuranetin, belonging to the flavonoid class, has the molecular formula C60. 16 H 14 O5 has a relative molecular mass of 286.08.

[0009] Thirdly, the present invention also provides the application of chlorpyrifos and chlorpyrifos in the control of wheat scab, wherein the pathogen of wheat scab is Fusarium graminearum as described in the first and / or second aspects. Fusarium graminearum ).

[0010] Furthermore, the aforementioned dimethyl ether and sakura extract achieve the control of wheat scab by inhibiting the mycelial growth of Fusarium graminearum, inhibiting the biosynthesis of deoxynivalenol, and / or inhibiting the expression of key genes for deoxynivalenol synthesis.

[0011] Furthermore, the key gene for deoxynivalenol synthesis is... TRI5 Genes and / or TRI6 Gene.

[0012] Furthermore, the concentration of the dimethyl ether used is not less than 25 μg / mL, and the concentration of the safflower extract used is not less than 25 μg / mL. This invention demonstrates through experiments that the inhibitory effect of dimethyl ether and / or safflower extract on the mycelial growth of Fusarium graminearum gradually increases with increasing concentration.

[0013] Fourthly, the present invention also provides a biological inhibitor for wheat scab, wherein the effective active ingredient of the biological inhibitor comprises koin dimethyl ether and / or sakura extract.

[0014] Furthermore, the concentration of the dimethyl ether and / or safflower extract used is not less than 25 μg / mL.

[0015] Fifthly, based on the same invention, the present invention also provides a composition for preventing and controlling wheat scab, comprising coumarin dimethyl ether and sakurain as active ingredients.

[0016] The present invention has the following beneficial effects: 1. This invention, through broad-target metabolomics analysis of wheat, for the first time screened and confirmed that coindimethyl ether and safflower extract have significant antibacterial activity against Fusarium graminearum. Indoor toxicity assays showed that coindimethyl ether had a half-maximal effective concentration (EC50) against the standard wild-type strain PH-1 of Fusarium graminearum. 50 The concentration of 58.01 μg / mL was found in safflower extract, and the EC50 of safflower extract was... 50 The concentration was 235.23 μg / mL, and both inhibitory effects on mycelial growth showed a significant concentration-dependent relationship.

[0017] 2. Under in vitro conditions, treatment with 30 μg / mL dimethyl ether and safflower extract alone did not significantly affect the toxin production capacity of mycelia per unit dry weight, but significantly reduced the total DON content in TBI medium, with inhibition rates of 30.34% and 32.19%, respectively. This indicates that both treatments indirectly reduced DON accumulation by inhibiting the growth of Fusarium graminearum, and have a practical effect on reducing toxin contamination.

[0018] 3. Gene expression analysis showed that treatment with 30 μg / mL safflower extract significantly inhibited key genes involved in DON synthesis. TRI5 The expression of [the substance] was inhibited at a rate of 26.47%, while that of [the substance] was inhibited by [a specific substance]. TRI5 and TRI6 The expression of [a specific substance] was not significantly affected. Combined with the results of Example 2, it can be seen that dimethyl ether mainly reduces DON content by inhibiting mycelial growth, while safflower extract, in addition to inhibiting growth, can also reduce DON content by downregulating [a specific mechanism]. TRI5 Gene expression directly interferes with the biosynthesis of DON, and the two mechanisms of action are complementary.

[0019] 4. In the mycelial growth inhibition experiment, the inhibition rate of the mixed treatment of 25 μg / mL dimethyl ether and safflower extract reached 34.33%, which was much higher than that of dimethyl ether alone (20.09%) and safflower extract alone (5.77%) at the same concentration, indicating that the combined use of the two has a synergistic effect on inhibiting the growth of Fusarium graminearum.

[0020] In the DON content inhibition experiment, the 30 μg / mL mixed treatment achieved an inhibition rate of 42.28% for DON, which was higher than that of dimethyl ether (30.34%) and safflower extract (32.19%) alone, further confirming that the two also have a synergistic effect in reducing toxin accumulation.

[0021] In in vivo wheat experiments, the 30 μg / mL mixed treatment reduced the DON content in wheat leaves by 52.12%, which was better than the 44.91% and 25.04% reductions of the individual treatments, indicating that it still maintains its synergistic effect under field application conditions.

[0022] 5. Safety assessment results showed that after treating healthy wheat with 30 μg / mL of dimethyl ether, safflower extract, and their mixture, no symptoms of phytotoxicity such as leaf yellowing, stunting, or curling were observed. Furthermore, the wheat fresh weight inhibition rate was negative, indicating that the three agents had no inhibitory effect on wheat growth and instead exhibited a certain degree of growth-promoting effect. This demonstrates that both agents have good biocompatibility at effective concentrations and are suitable for further development and application as green pesticides.

[0023] 6. Both dimethyl ether and safflower extract are plant-derived natural products with low toxicity, easy degradation, and high environmental compatibility. The experimental data provided by this invention fully demonstrate their effectiveness in inhibiting the growth of Fusarium graminearum and the accumulation of DON, and they are safe and harmless to wheat. They can serve as a green alternative or supplement to chemical fungicides, providing a new solution for the green control of wheat scab, and have significant application value and promising prospects for promotion. Attached Figure Description

[0024] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only one embodiment of the present invention. For those skilled in the art, other embodiments can be derived from the provided drawings without creative effort.

[0025] Figure 1 Effects of dimethyl ether on the mycelial growth of Fusarium graminearum, with concentrations from left to right being 0, 5, 10, 25, 50, and 75 μg / mL; Figure 2The effect of sakura extract on the mycelial growth of Fusarium graminearum, with concentrations from left to right being 0, 5, 10, 25, 50, and 75 μg / mL; Figure 3 Effects of dimethyl ether and sakura extract on the mycelial growth of Fusarium graminearum, with concentrations from left to right being 0 and 25 μg / mL; Figure 4 Effects of dimethyl ether and safflower extract on DON synthesis (A) and DON content (B) in vitro and in vitro. Figure 5 Coenyl dimethyl ether and sakurain are key genes in DON synthesis. TRI5 (A) and TRI6 (B) The impact of expression; Figure 6 Effects of dimethyl ether and sakura extract on DON content in wheat; Figure 7 The effects of dimethyl ether and safflower extract on wheat growth, from left to right: CK, dimethyl ether, safflower extract, dimethyl ether + safflower extract. Detailed Implementation

[0026] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of them. The following embodiments are only for illustrative purposes and are not intended to limit the scope of the present invention in any way. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention. Unless otherwise specified, the experimental methods used in the present invention are conventional methods. Unless otherwise specified, the materials and reagents used in the present invention are commercially available. Furthermore, other terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those skilled in the art.

[0027] This invention screened endogenous wheat metabolites whose levels were significantly upregulated compared to the control group by measuring the broad-target metabolome of wheat ears infected with Fusarium graminearum. A total of 652 endogenous metabolites with significant changes in content were detected, of which 396 showed significant upregulation. Subsequently, based on factors such as the upregulation factor and cost, 15 endogenous metabolites were selected for further experiments, of which 5 showed inhibitory effects on Fusarium graminearum mycelial growth or DON synthesis (Tables 1 and 2).

[0028] Table 1. Effects of 15 endogenous wheat metabolites on the mycelial growth of Fusarium graminearum.

[0029] Table 2. Effects of 15 wheat endogenous metabolites on DON biosynthesis in Fusarium graminearum

[0030] This invention investigated the application of wheat endogenous metabolites koin dimethyl ether and safflower extract in preventing wheat scab and the fungal toxin deoxynivalenol (DON). The study showed that koin dimethyl ether and safflower extract inhibited Fusarium graminearum mycelial growth, DON biosynthesis, and the relative expression levels of key DON synthesis genes under in vitro conditions.

[0031] Example 1: Indoor toxicity determination of coumarin dimethyl ether and safflower extract against Fusarium graminearum 1.1 Experimental Materials and Methods The metabolites used in this invention are cherrysin dimethylether (Shanghai Aladdin Biochemical Technology Co., Ltd.) and sakuranetin (Shanghai Maclean Biochemical Technology Co., Ltd.). The culture medium is PDA medium (200 g potato, 20 g glucose, 15 g agar powder, 1 L deionized water), and the strain is the standard wild-type strain of Fusarium graminearum. Fusarium graminearum PH-1 (purchased from Hangzhou Baosai Biotechnology Co., Ltd.)

[0032] This invention uses the mycelial growth rate method to determine the antibacterial activity of dimethyl ether (DMSO) and safflower extract against *Fusarium graminearum*. DMSO and safflower extract were dissolved in DMSO and diluted to different concentrations, then added to different PDA media to form treatment groups with different concentration gradients (0, 5, 10, 25, 50, 75 μg / mL). An equal volume of DMSO was added to the control group. Each treatment was repeated three times. The media were then poured into disposable petri dishes with a diameter of 9 cm. After cooling and solidification, *Fusarium graminearum* activated for 4 days was used to create colony discs at the edge of the colony using a 0.6 cm diameter punch and inoculated into the media containing different concentrations of DMSO or safflower extract. The media were incubated upside down in a 25°C incubator in the dark for 4 days. The colony diameter of each treatment and the control group was measured using the cross-cross method, and the inhibition rates of DMSO and safflower extract on mycelial growth were calculated.

[0033] Mycelial growth inhibition rate (%) = (Coronary diameter of control group - Colony diameter of treatment group) / (Coronary diameter of control group) 0.6) × 100%.

[0034] 1.2 Experimental Results Using the logarithm of the concentration of different treatment groups as x and the probability value of mycelial growth inhibition rate as y, a linear regression equation was established, and the results are shown in Table 3.

[0035] Table 3. Results of indoor toxicity assays of coumarin and safflower extract against Fusarium graminearum.

[0036] The experimental results of cyclohexane are as follows: Figure 1 As shown, the colony diameter of Fusarium graminearum decreases with increasing concentration of dimethyl ether, indicating that the inhibitory effect of dimethyl ether on its mycelial growth gradually increases with increasing concentration.

[0037] The experimental results of cherry blossom extract are as follows: Figure 2 As shown, the colony diameter of Fusarium graminearum decreases with increasing concentration of safflower extract, indicating that the inhibitory effect of safflower extract on mycelial growth gradually increases with increasing concentration.

[0038] Table 3 shows that the EC50 of cyclophosphamide for Fusarium graminearum (PH-1) is... 50 The EC50 of safflower extract against Fusarium graminearum (PH-1) was 58.01 μg / mL. 50 The concentration was 235.23 μg / mL, indicating that both dimethyl ether and safflower extract had good inhibitory effects on the mycelial growth of Fusarium graminearum, and that dimethyl ether had a better inhibitory effect on the growth of Fusarium graminearum than safflower extract.

[0039] Furthermore, this invention further validated the effect of a mixture of 25 μg / mL of koin dimethyl ether and safflower extract on the mycelial growth of Fusarium graminearum (using a gradient concentration intermediate to compare the effect of a single metabolite versus a mixture of two metabolites on the growth of Fusarium graminearum at PH-1). Figure 3 The experimental results are shown in Table 4 and Figure 3 As shown, the inhibition rate of the growth of Fusarium graminearum after mixing the two metabolites was much greater than that of either metabolite alone, indicating that the mixture of the two metabolites can further enhance the inhibition of Fusarium graminearum mycelial growth.

[0040] Table 4. Inhibition of Fusarium graminearum (PH-1) growth by coumarin dimethyl ether, safflower extract, and a mixture of their two metabolites.

[0041] Example 2: Indoor and in vitro effects of dimethyl ether and sakura extract on DON production 2.1 Experimental Materials and Methods The culture medium used in this invention: CMC medium (15 g carboxymethyl cellulose, 2 g NaNO3, 0.5 g MgSO4·7H2O, 1 g KH2PO4, 1 g Yeast Extract, 1 L deionized water) was used to culture spores; TBI medium (30 g sucrose, 1 g KH2PO4, 0.5 g MgSO4·7H2O, 0.5 g KCl, 0.01 g FeSO4·7H2O, 0.8 g putrescine, 200 μL trace element B, 1 L deionized water) was used to induce Fusarium graminearum to produce DON; Trace element B (100 mL) contains 5 g Citric acid, 5 g ZnSO4·7H2O, 0.25 g CuSO4·5H2O, 0.05 g MnSO4·H2O, 0.05 g H3BO4, and 0.05 g Na2MoO4·2H2O.

[0042] Five fungal discs were punched from the edge of the growing colony using a puncher and transferred to CMC medium. The culture was incubated at 25 °C in the dark with shaking at 200 rpm for 5 days. The resulting fungal culture was filtered to obtain a spore suspension, which was then adjusted to a final concentration of 1 × 10⁻⁶. 6 / mL. Take 1 mL of the spore suspension and inoculate it into a 50 mL Erlenmeyer flask containing TBI medium. All Erlenmeyer flasks are placed in a 28°C incubator in the dark. After 2 days of culture, add certain concentrations of cyclohexane, safflower extract, and a mixture of the two to the medium to achieve a concentration of 30 μg / mL for each of the three substances (considering solubility issues, and safflower extract EC 10 ... 50 The values ​​are relatively large, and it is not possible to guarantee that both metabolites will reach EC levels. 50 The concentration was determined by the concentration of DMSO, therefore 30 μg / mL was chosen as the uniform experimental concentration. An equal volume of DMSO was added to the blank control group. After 4 days of incubation, 1 mL of culture medium was extracted from 50 mL of TBI medium and filtered through a 0.22 μm aqueous filter membrane into a brown vial for subsequent HPLC analysis. The remaining culture medium was vacuum filtered, dried, and weighed. All treatments were performed in triplicate.

[0043] DON content reduction rate (%) = (DON content in control group - DON content in treatment group) / DON content in control group × 100% Toxin production per unit dry weight mycelium (μg / g) = Total DON content in TBI medium / Mycelium per unit dry weight The test reagents and test strains used in Example 2 were from the same sources as those in Example 1.

[0044] 2.2 Experimental Results Experimental results are as follows Figure 4As shown, the significance analysis of the control and treatment groups using independent samples t-tests revealed that although neither 30 μg / mL of dimethyl ether nor safflower extract significantly inhibited the toxin production per unit dry weight of mycelium, they significantly reduced the DON content in the culture medium, with inhibition rates of 30.34% and 32.19%, respectively. This indicates that dimethyl ether and safflower extract can reduce DON content by inhibiting the growth of Fusarium graminearum. Furthermore, the combined treatment of dimethyl ether and safflower extract also significantly reduced the DON content in TBI culture medium, with an inhibition rate of 42.28%, which is greater than the inhibition rates of the two metabolites treated individually. This suggests that the combination of dimethyl ether and safflower extract is more effective in inhibiting DON content.

[0045] Example 3: The effects of coucin dimethyl ether and sakurain on key toxin-producing genes in Fusarium graminearum TRI5 , TRI6 The effect of expression on measurement 3.1 Experimental Materials and Methods Using a punch, collect fungal discs from the edge of the growing colony and transfer them to CMC medium. Incubate at 25°C in the dark with shaking at 200 rpm for 5 days. Filter the resulting fungal culture to obtain a spore suspension, then adjust to a final concentration of 1×10⁻⁶. 6 / mL. 1 mL of the spore suspension was inoculated into a 50 mL Erlenmeyer flask containing TBI medium. All flasks were incubated at 28°C in the dark. After 2 days of incubation, specific concentrations of dimethyl ether (DMSO) and safflower extract were added to the medium to achieve concentrations of 30 μg / mL for each, respectively. An equal volume of DMSO was added to the blank control group. After 4 days of incubation, 50 mL of TBI medium was vacuum filtered under reduced pressure, flash-frozen in liquid nitrogen, and stored at -80°C. All treatments were performed in triplicate.

[0046] Gene expression inhibition rate (%) = (relative gene expression level in control group - relative gene expression level in treatment group) / relative gene expression level in control group × 100%.

[0047] The culture medium, reagents, and test strains used in Example 3 were the same as those in Example 2.

[0048] 3.2 Experimental Results Coin dimethyl ether and sakurain target key toxin-producing genes in Fusarium graminearum. TRI5 , TRI6 The experimental results of the effect of relative expression level are as follows Figure 5 As shown, under indoor in vitro conditions, the effect of dimethyl ether treatment on... TRI5 and TRI6 The relative expression level of [the substance] was not significantly affected, while [the substance] significantly inhibited [the expression of] [the substance]. TRI5The relative expression level was 26.47%, with an inhibition rate of 26.47%. Combined with the analysis in Example 2, it can be seen that dimethyl ether and sakurain mainly reduce the DON content in the culture medium by inhibiting the mycelial growth of Fusarium graminearum.

[0049] Example 4: Determination of the effects of dimethyl ether and sakura extract on DON content in wheat 4.1 Experimental Materials and Methods The wheat variety used in this invention is Jimai 22, a semi-winter wheat widely planted in the Huang-Huai-Hai region.

[0050] Using a punch, collect fungal discs from the edge of the growing colony and transfer them to CMC medium. Incubate at 25°C in the dark with shaking at 200 rpm for 5 days. Filter the resulting fungal culture to obtain a spore suspension, then adjust to a final concentration of 1×10⁻⁶. 5 / mL. 10 μL was inoculated onto wheat leaves, followed by spraying with water and covering with a bag to maintain humidity. Three days later, 30 μg / mL of dimethyl ether, cherry blossom extract, and a mixture thereof were sprayed onto the wheat leaves. The control group was treated with water. Five days after the first spraying, the same concentration of dimethyl ether, cherry blossom extract, and a mixture thereof was repeated. Four days after the second spraying, the wheat leaves were removed with scissors, flash-frozen in liquid nitrogen, and stored at -80℃. DON was extracted from the wheat leaves using the QuEChERS method, and the DON content was determined by HPLC.

[0051] DON content inhibition rate (%) = (DON content per unit weight of wheat in the control group - DON content per unit weight of wheat in the treatment group) / DON content per unit weight of wheat in the control group × 100% The culture medium, reagents, and test strains used in Example 4 were the same as those in Example 2.

[0052] 4.2 Experimental Results The effects of dimethyl ether, safflower extract, and mixtures thereof on the DON content in wheat are as follows: Figure 6 As shown, treatment with 30 μg / mL dimethyl ether and safflower extract after Fusarium graminearum infection of wheat significantly inhibited the DON content in wheat leaves, with inhibition rates of 44.91% and 25.04%, respectively. These results indicate that dimethyl ether and safflower extract can effectively inhibit the DON content in wheat in outdoor in vivo experiments, reducing DON pollution and mitigating its harm to host crops such as wheat.

[0053] Furthermore, treating wheat infected with Fusarium graminearum with a mixture of 30 μg / mL dimethyl ether and 30 μg / mL sakura extract reduced the DON content in wheat by 52.12%, and the inhibitory effect of the mixture on the DON content in wheat was stronger than that of either metabolite alone.

[0054] Example 5: Evaluation of the effects of dimethyl ether and safflower extract on wheat growth 5.1 Experimental Materials and Methods A concentration of 30 μg / mL of quinine dimethyl ether, safflower extract, or a mixture thereof was sprayed onto healthy wheat leaves with uniform growth. The control group was treated with water. The spraying was repeated once after 5 days. Four days after the second spraying, the wheat growth was observed and the fresh weight of the wheat was weighed to calculate the fresh weight inhibition rate.

[0055] Fresh weight inhibition rate (%) = (fresh weight of wheat leaves in control group - fresh weight of wheat leaves in treatment group) / fresh weight of wheat leaves in control group × 100%.

[0056] The reagents used in Example 5 are the same as those in Example 1.

[0057] 5.2 Experimental Results The effects of treatments with 30 μg / mL concentrations of quinine dimethyl ether, safflower extract, and a mixture thereof on wheat growth are as follows: Figure 5 As shown in Table 5, the results indicate that treatment with 30 μg / mL of quinine dimethyl ether, cherry blossom extract, and their mixture did not result in significant stunting, lodging, yellowing, chlorosis, wrinkling, or curling of wheat leaves, and the fresh weight of wheat increased compared to the control group. These results suggest that treatment with 30 μg / mL of quinine dimethyl ether, cherry blossom extract, and their mixture has a certain promoting effect on wheat growth.

[0058] Table 5. Effects of dimethyl ether, safflower extract, and mixtures thereof on wheat fresh weight.

[0059] It is understood that those skilled in the art can make equivalent substitutions or modifications to the technical solutions and concepts of this invention, and all such substitutions or modifications should fall within the protection scope of the appended claims.

Claims

1. Coine dimethyl ether is used as a plant pathogen Fusarium graminearum (… Fusarium graminearum Uses of antibacterial agents.

2. Cherry blossom extract is used as a plant pathogen by Fusarium graminearum (… Fusarium graminearum Uses of antibacterial agents.

3. The application of chlorpyrifos and chlorpyrifos in the control of wheat scab, characterized in that, The pathogen causing wheat scab is Fusarium graminearum as described in claim 1 or claim 2. Fusarium graminearum ).

4. The application of ketamine and safflower extract according to claim 3 in the control of wheat scab, characterized in that, The aforementioned dimethyl ether and sakurain control wheat scab by inhibiting the mycelial growth of Fusarium graminearum, inhibiting the biosynthesis of deoxynivalenol, and / or inhibiting the expression of key genes for deoxynivalenol synthesis.

5. The application of ketamine and safflower extract according to claim 4 in the control of wheat scab, characterized in that, The key gene for deoxynivalenol synthesis is... TRI5 Genes and / or TRI6 Gene.

6. The application of quinine dimethyl ether and safflower extract according to claim 3 in the control of wheat scab, characterized in that, The concentration of the dimethyl ether used is not less than 25 μg / mL, and the concentration of the sakura extract used is not less than 25 μg / mL.

7. A biological inhibitor for wheat scab, characterized in that, The active ingredients of the bioinhibitor include coenzyme dimethyl ether and / or sakura extract.

8. A biological inhibitor for wheat scab according to claim 7, characterized in that, The concentration of the dimethyl ether and / or safflower extract used shall not be less than 25 μg / mL.

9. A composition for controlling wheat scab, characterized in that, It contains dimethyl ether and / or sakura extract as active ingredients.

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