Inlet marine fish pathogenic parasite sampling and detecting device

Abstract

The invention belongs to the technical field of parasite sampling, particularly relates to an imported marine fish pathogenic parasite sampling and detecting device, and provides the following scheme aiming at the problems of low manual sampling efficiency, inaccurate positioning and easy sample damage in existing marine fish parasite quarantine. Comprising a sampling base, a movable supporting plate, a sampling area identification assembly, a sampling pipe and a negative pressure separation assembly. The sampling area identification assembly can losslessly and accurately position a suspected parasite infection area in a fish body. The sampling pipe is provided with an annular gas guide cavity and an annular liquid guide cavity, sterile gas and normal saline can be sprayed cooperatively, and minimally invasive and efficient stripping and recycling of insect bodies are achieved. The negative pressure separation assembly adopts a double-chamber design, a micro-filtration membrane is arranged in the negative pressure separation assembly, a sample can be shunted and independently treated in single operation, and a negative pressure liquid extraction pipe of the negative pressure separation assembly has an automatic valve function of negative pressure ventilation and normal pressure closing, so that subsequent in-situ fixation is facilitated. According to the invention, the whole process automation from precise minimally invasive sampling to automatic separation and fixation is realized.

Description

Technical Field

[0001] This invention relates to a sampling and detection device, specifically a sampling and detection device for pathogenic parasites in imported marine fish, belonging to the field of parasite sampling technology. Background Technology

[0002] With the deepening of global trade integration and the advancement of the Belt and Road Initiative, my country's imports of aquatic products, especially frozen Alaska pollock and other marine fish from the Russian Far East and the North Pacific Ocean, have continued to grow. These types of fish are common hosts for foodborne parasites such as Anisakis, and human consumption of contaminated fish can lead to Anisakiasis, seriously threatening public health and trade security. Therefore, efficient and accurate parasite quarantine of imported marine fish is crucial. However, the existing technological system faces a series of bottlenecks throughout the entire process from testing to sampling, making it difficult to meet the needs of rapid customs clearance and precise disease control at ports.

[0003] Existing parasite detection technologies can be mainly divided into two categories: laboratory testing and preliminary screening, but both have significant limitations. In laboratory testing, for example, the enzyme-linked immunosorbent assay (ELISA) method for detecting Anisakis in seafood, disclosed in publication number CN101603964A, involves preparing specific antibodies and using ELISA to detect parasite antigens in the sample. While this method has high sensitivity, it is essentially an offline and destructive biochemical detection method. It heavily relies on manual dissection, separation, and fragmentation of the parasite from the fish to obtain antigens. This process itself presents the challenge of efficiently and accurately locating and obtaining parasite samples hidden deep within the fish's muscles or abdominal cavity. Another example is the TaqMan PCR detection method for Anisakis simplex in seafood, disclosed in publication number 103361401A, which requires a high-quality parasite or DNA sample as input. Traditional sampling methods represent the weakest and non-automatable link in this chain. Currently, port quarantine mainly relies on manual visual inspection and dissection, a method that is extremely inefficient, labor-intensive, significantly influenced by personnel experience, and prone to missed detections. Furthermore, there is a lack of integrated solutions for the rapid and parallel pretreatment of the mixed suspension containing the parasites (such as differential fixation for different subsequent detection purposes). Existing devices are limited in function, the sampling process is rough, often causing damage to the parasites or sample contamination, and they cannot achieve "one sample, multiple samples" to meet the needs of various subsequent analyses such as morphological identification and molecular biological detection. Summary of the Invention

[0004] This invention provides a sampling and detection device for pathogenic parasites in imported marine fish to solve the technical problems of low efficiency, inaccurate positioning, and easy damage of samples in the existing quarantine of marine fish parasites.

[0005] The present invention achieves the above objectives through the following technical solution: a sampling and detection device for pathogenic parasites in imported marine fish, comprising a sampling base, a sampling tube and a negative pressure separation component connected on the sampling base, a tray movably provided on the sampling base, and a sampling area identification component located directly above the moving path of the tray. The sampling area identification components include a light curtain sensor, a near-infrared light source, and a multispectral camera; The sampling tube is provided with a first variable diameter tube body, a second variable diameter tube body and a piercing needle in sequence. An annular air guiding cavity is opened at the docking part of the sampling tube and the first variable diameter tube body, and the air in the annular air guiding cavity flows into the sampling area. An annular liquid guiding cavity is opened at the docking part of the first variable diameter tube body and the second variable diameter tube body, and the liquid in the annular liquid guiding cavity flows into the sampling area. The negative pressure separation assembly includes a filter tube, a microfiltration tube, and a liquid collection tube arranged sequentially from top to bottom. The filter tube is equipped with a filter screen, and the microfiltration tube has two independent cavities, each of which is equipped with a microfiltration membrane. The bottom of each cavity of the microfiltration tube is connected to a negative pressure liquid extraction tube. The negative pressure liquid extraction tube is in a closed loop when it is under negative pressure and in a closed loop when it is under normal pressure.

[0006] As a further embodiment of the present invention: the sampling base has a long strip-shaped limiting groove, the bottom surface of the support plate is connected to a slider, and the slider is slidably disposed in the limiting groove. A drive motor is embedded in one side wall of the limiting groove, and a lead screw is coaxially fixedly connected to the motor shaft of the drive motor. The lead screw thread passes through the slider, and several balls are embedded in both sides of the bottom surface of the support plate.

[0007] As a further embodiment of the present invention: the sampling area identification component also includes an identification cover, and the light curtain sensor, near-infrared light source and multispectral camera are all integrated and installed on the inner top surface of the identification cover. The near-infrared light source is arranged in a ring shape, and the multispectral camera is installed at the center of the near-infrared light source. There are notches on both sides of the identification cover. The plate body is U-shaped, and when the plate body moves to directly under the identification cover, the two sides of the plate body engage with the notches in the identification cover body.

[0008] As a further embodiment of the present invention: a six-axis robotic arm is installed on the sampling base, and the installation position of the six-axis robotic arm is located on one side of the pallet moving path, and the sampling tube is clamped and fixed at the movable front end of the six-axis robotic arm.

[0009] As a further embodiment of the present invention: the upper end of the sampling tube is connected to an air inlet connector and a liquid inlet connector. A liquid delivery channel is opened inside the sampling tube, connecting the liquid inlet connector and the annular liquid guiding cavity. A plurality of annular liquid-passing micro-holes are opened on the inclined surface of the sampling tube and the first variable diameter tube, and the plurality of liquid-passing micro-holes are connected to the annular liquid guiding cavity. An air delivery channel is opened inside the sampling tube and the first variable diameter tube, connecting the air inlet connector and the annular air guiding cavity. A plurality of annular air-passing micro-holes are opened on the inclined surface of the first variable diameter tube and the second variable diameter tube, and the plurality of air-passing micro-holes are connected to the annular air guiding cavity.

[0010] As a further embodiment of the present invention: the negative pressure separation assembly also includes an upper cover disposed at the top of the filter tube, and an infusion tube is connected between the upper cover and the sampling tube. The upper cover, the filter tube, the microfiltration tube and the collection tube are connected by threads, and a sealing ring is fitted at the connection part of each tube.

[0011] As a further embodiment of the present invention: the negative pressure separation component also includes a positioning support plate. The two ends of the positioning support plate are fixedly connected to the sampling base with vertically arranged support rods. A limiting groove is opened in the middle part of the positioning support plate. Positioning grooves are provided on both sides of the limiting groove. The filter tube is fixedly connected to a limiting ring and a positioning block. The positioning block is located below the limiting ring. When the filter tube is inserted into the limiting groove, the limiting ring is locked above the limiting groove, and the positioning block is locked in the positioning groove.

[0012] As a further embodiment of the present invention: an outer ring of a mesh body is movably fitted inside the filter tube, the filter mesh is connected to the outer ring of the mesh body, a liquid-collecting bottom plate is fixedly connected inside the microfiltration tube, a lower connecting partition is also fixedly connected inside the microfiltration tube along the diameter direction, the lower connecting partition is fixedly connected to the liquid-collecting bottom plate, an upper connecting partition is fixedly connected below the outer ring of the mesh body, a slot is opened on the bottom edge of the upper connecting partition, the lower connecting partition is inserted into the slot when the filter tube and the microfiltration tube are in the docking state, a microfiltration bottom mesh is also connected inside the microfiltration tube, the microfiltration bottom mesh is located on both sides of the lower connecting partition, and the microfiltration membrane is laid on the microfiltration bottom mesh.

[0013] As a further embodiment of the present invention: the liquid-collecting bottom plate is connected to two negative pressure liquid-drawing pipes, and the two negative pressure liquid-drawing pipes correspond to two independent cavities separated by the lower connecting partition. An inner cylinder is movably connected inside the negative pressure liquid-drawing pipe, and a retaining ring is connected to the cylinder body of the inner cylinder. A concave groove is opened on the inner wall of the negative pressure liquid-drawing pipe, and the retaining ring is locked in the concave groove. A compression spring is also sleeved on the cylinder body of the inner cylinder. The two ends of the compression spring abut against the bottom surface of the groove of the retaining ring and the groove, respectively. A drain port is opened at the lower end of the cylinder body, and the bottom surface of the inner cylinder is on the same plane as the bottom end of the negative pressure liquid-drawing pipe in the initial state.

[0014] As a further embodiment of the present invention: when the microfiltration tube is in the negative pressure separation sampling state, its bottom end is connected to the liquid collection tube, and the liquid collection tube is connected to the external negative pressure pump by a negative pressure connector, and the negative pressure connector is connected to the upper side wall of the liquid collection tube. When the microfiltration tube is in the sampling completed state, its bottom end is connected to a movable bottom cover, and a stop block is connected inside the movable bottom cover. The stop block abuts against the bottom of the negative pressure suction tube when the microfiltration tube and the movable bottom cover are connected.

[0015] The beneficial effects of this invention are: 1. This invention comprises a sampling base, a connected sampling tube, and a negative pressure separation component. The sampling base is movably equipped with a tray, and a sampling area identification component is located directly above the tray's movement path. The sampling base serves as the support and working platform of the device, with the connected sampling tube and negative pressure separation component ensuring the closed nature of the operation process and effectively reducing the risk of sample exposure during transfer. The tray carries the imported marine fish samples to be tested, and its movable design allows a single fish sample to be transported in an orderly manner to different workstations, such as passing through the identification area before reaching the sampling position, thus modularizing the testing process and improving the efficiency of large-scale sample processing. The sampling area identification component located directly above the tray's movement path first scans and analyzes the fish body that moves below it to determine the specific coordinates of suspected parasite infection, and then guides the subsequent sampling action, upgrading the sampling behavior from random search to precise positioning, greatly improving the sampling efficiency of target parasites. 2. The sampling area identification component of this invention includes a light curtain sensor, a near-infrared light source, and a multispectral camera. The light curtain sensor, as the first layer sensor, can quickly acquire the three-dimensional contour, size, and real-time position and posture information of the fish body on the tray through its scanning plane, providing a spatial reference for all subsequent coordinate transformations. The near-infrared light source is an active illumination source that emits near-infrared light of a specific wavelength to irradiate the fish surface, providing stable and controllable excitation conditions for spectral analysis and overcoming interference from ambient light changes. Under the illumination of the near-infrared light source, the multispectral camera simultaneously captures multi-band spectral images reflected from the fish surface. By analyzing the differences in characteristic absorption and reflection in the near-infrared band of different tissues such as normal fish meat, fat, connective tissue, and parasites or their cysts, it identifies suspected parasite-rich areas that are difficult to detect with the naked eye. The precise geometric coordinates provided by the light curtain sensor and the coordinates of the suspected target image provided by the multispectral camera ultimately output a precise aiming point in three-dimensional space, thereby achieving accurate minimally invasive sampling. 3. The sampling tube of this invention is sequentially provided with a first variable-diameter tube body, a second variable-diameter tube body, and a piercing needle. An annular air-guiding cavity is formed at the joint between the sampling tube and the first variable-diameter tube body, and air from the annular air-guiding cavity flows into the sampling area. An annular liquid-guiding cavity is formed at the joint between the first and second variable-diameter tube bodies, and liquid from the annular liquid-guiding cavity flows into the sampling area. The piercing needle is located at the foremost point and is used to pierce the target fish tissue located by the sampling area identification component. The annular liquid-guiding cavity can introduce sterile saline or other liquids into the sampling area. In the sampling area, the injection of liquid serves to flush, suspend, and loosen parasite larvae tightly attached to the tissue. The annular air-conducting cavity can introduce sterile gases such as CO2 or N2 into the sampling area. The gas jet can further assist in disturbing the interstitial spaces and effectively prevent loose tissue fragments from clogging the aspiration port of the puncture needle. The synergistic effect of liquid and gas creates a dynamic gas-liquid flow environment around the needle tip, which facilitates the detachment of parasite larvae from biological tissue. The detached larvae and the liquid mixture suspension are then recovered through the sampling tube under the action of negative suction pressure. 4. The negative pressure separation component of this invention includes a filter tube, a microfiltration tube, and a collection tube arranged sequentially from top to bottom. The filter tube contains a filter screen, and the microfiltration tube contains two independent cavities, each containing a microfiltration membrane. Each cavity of the microfiltration tube is connected to a negative pressure suction tube at its bottom. The negative pressure suction tube is open when under negative pressure and closed when under normal pressure. The filter screen in the filter tube serves as the first stage of filtration, primarily intercepting larger tissue fragments, mucus clumps, and other impurities in the sample suspension, thus providing preliminary purification. The microfiltration tube contains two... The system features independent cavities, each equipped with a microfiltration membrane. This allows parasite samples separated from the same original sampling suspension to be processed in parallel, significantly improving the utilization rate of a single sampling. The negative pressure suction tube connected to the bottom of each cavity is designed to be open under negative pressure and closed under normal pressure. During the filtration and separation stage, negative pressure is applied to keep the tube unobstructed, allowing liquid to be drawn through the microfiltration membrane while the parasites are retained. When in-situ fixation or sampling is required after separation, the negative pressure is released, and the tube automatically closes to prevent backflow of fixative or air. The collection tube collects all filtered waste liquid. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the initial state structure of the device of the present invention; Figure 2 A schematic diagram of the structure of the device of the present invention in preparation for puncture sampling. Figure 3 This is a schematic diagram of the internal structure of the outer casing of the present invention; Figure 4 This is a schematic diagram of the side cross-sectional structure of the sampling base of the present invention; Figure 5This is a schematic diagram of the sampling tube structure of the present invention; Figure 6 This is a schematic diagram of the cross-sectional structure of the sampling tube of the present invention; Figure 7 For the present invention Figure 6 Schematic diagram of the structure at point A in the middle; Figure 8 For the present invention Figure 6 Schematic diagram of the structure at point B; Figure 9 This is a schematic cross-sectional view of the connection between the positioning support plate and the filter tube in this invention. Figure 10 This is a schematic diagram of the split structure of the negative pressure separation component of the present invention when it is in the negative pressure separation sampling state; Figure 11 This is a schematic diagram of the split structure of the negative pressure separation component of the present invention when the sampling is completed; Figure 12 This is a schematic diagram of the internal structure of the filter tube of the present invention; Figure 13 This is a schematic diagram of the internal structure of the microfiltration tube of the present invention; Figure 14 This is a schematic diagram of the connection structure between the polymer base plate and the negative pressure extraction pipe of the present invention; Figure 15 This is a schematic diagram of the internal split structure of the negative pressure suction tube of the present invention.

[0017] In the diagram: 1. Sampling base; 11. Limiting groove; 12. Drive motor; 13. Lead screw; 2. Support plate; 21. Ball bearing; 22. Slider; 3. Identification cover; 31. Light curtain sensor; 32. Near-infrared light source; 33. Multispectral camera; 4. Six-axis robotic arm; 5. Sampling tube; 51. First variable diameter tube body; 52. Second variable diameter tube body; 53. Piercing needle; 54. Air inlet connector; 55. Liquid inlet connector; 56. Gas delivery channel; 57. Annular air guide cavity; 58. Liquid delivery channel; 59. Annular liquid guide cavity; 510. Ventilation micropore; 511. Liquid delivery micropore; 6. Negative pressure separation component; 61. Positioning support plate; 62. Top cover 63. Filter tube; 64. Microfiltration tube; 65. Liquid collection tube; 66. Support rod; 67. Limiting ring; 68. Positioning block; 69. Limiting groove; 610. Positioning groove; 611. Negative pressure connector; 612. Movable bottom cover; 613. Abutment block; 614. Outer ring of mesh body; 615. Filter screen; 616. Liquid collection base plate; 617. Microfiltration bottom screen; 618. Microfiltration membrane; 619. Negative pressure suction tube; 620. Lower connecting partition; 621. Inner cylinder; 622. Snap ring; 623. Compression spring; 624. Drain port; 625. Upper connecting partition; 626. Slot; 627. Concave slot; 7. Infusion tube; 8. Sealing ring. Detailed Implementation

[0018] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] Example 1 like Figures 1 to 15 As shown, an imported marine fish pathogenic parasite sampling and detection device includes a sampling base 1, on which a connected sampling tube 5 and a negative pressure separation component 6 are mounted. A tray 2 is movably mounted on the sampling base 1, and a sampling area identification component is positioned directly above the moving path of the tray 2. The sampling base 1 serves as the support and working platform of the device, with the connected sampling tube 5 and negative pressure separation component 6 ensuring the closed nature of the operation process and effectively reducing the risk of sample exposure during transfer. The tray 2 carries the imported marine fish samples to be tested. Its movable design allows a single fish sample to be transported in an orderly manner to different workstations, such as first passing through the identification area and then reaching the sampling position, achieving modularization of the detection process and improving the efficiency of large-scale sample processing. The sampling area identification component positioned directly above the moving path of the tray 2 first scans and analyzes the fish body moving below it to determine the specific coordinates of suspected parasite infection, and then guides the subsequent sampling action, upgrading the sampling behavior from random searching to precise positioning, greatly improving the sampling efficiency of target parasites. The sampling area identification component includes a light curtain sensor 31, a near-infrared light source 32, and a multispectral camera 33. The light curtain sensor 31, as the first-layer sensor, can quickly acquire the three-dimensional contour, size, and real-time position and posture information of the fish body on the tray 2 through its scanning plane, providing a spatial reference for all subsequent coordinate transformations. The near-infrared light source 32 is an active illumination source that emits near-infrared light of a specific wavelength to irradiate the fish surface, providing stable and controllable excitation conditions for spectral analysis and overcoming interference from ambient light changes. Under the illumination of the near-infrared light source 32, the multispectral camera 33 simultaneously captures multi-band spectral images reflected from the fish surface. By analyzing the differences in characteristic absorption and reflection in the near-infrared band of different tissues such as normal fish meat, fat, connective tissue, and parasites or their cysts, it identifies suspected parasite-rich areas that are difficult to detect with the naked eye. The precise geometric coordinates provided by the light curtain sensor 31 and the suspected target image coordinates provided by the multispectral camera 33 ultimately output a precise aiming point in three-dimensional space, thereby achieving accurate minimally invasive sampling. The sampling tube 5 has a first variable diameter tube body 51, a second variable diameter tube body 52, and a piercing needle 53 arranged sequentially. An annular air guiding cavity 57 is formed at the joint between the sampling tube 5 and the first variable diameter tube body 51, and the air in the annular air guiding cavity 57 flows into the sampling area. An annular liquid guiding cavity 59 is formed at the joint between the first variable diameter tube body 51 and the second variable diameter tube body 52, and the liquid in the annular liquid guiding cavity 59 flows into the sampling area. The piercing needle 53 is located at the foremost end and is used to pierce the target fish tissue located by the sampling area identification component. The annular liquid guiding cavity 59 can allow sterile saline and other liquids to pass through it. The cavity is introduced into the sampling area. The injection of liquid serves to flush, suspend, and loosen the parasitic larvae tightly attached to the tissue. The annular air guide cavity 57 can introduce sterile gas such as CO2 or N2 into the sampling area. The gas jet can further assist in disturbing the interstitial spaces and effectively prevent loose tissue fragments from clogging the aspiration port of the puncture needle 53. The synergistic effect of liquid and gas creates a dynamic gas-liquid flow environment around the needle tip, which facilitates the removal of parasitic larvae from biological tissue. The mixed suspension of the removed larvae and liquid is then recovered through the sampling tube 5 under the action of suction negative pressure. The negative pressure separation component 6 includes a filter tube 63, a microfiltration tube 64, and a collection tube 65 arranged sequentially from top to bottom. The filter tube 63 contains a filter screen 615. The microfiltration tube 64 has two independent cavities, each containing a microfiltration membrane 618. Each cavity of the microfiltration tube 64 is connected to a negative pressure suction tube 619 at its bottom. The negative pressure suction tube 619 is open when under negative pressure and closed when under normal pressure. The filter screen 615 in the filter tube 63 serves as the first stage of filtration, primarily intercepting larger tissue fragments, mucus clumps, and other impurities in the sample suspension, thus providing preliminary purification. The microfiltration tube... The internal structure 64 has two independent cavities, each containing a microfiltration membrane 618. This allows parasite samples separated from the same original sampling suspension to be processed separately in parallel, greatly improving the sample utilization rate of a single sampling. The negative pressure suction tube 619 connected to the bottom of each cavity is designed to be open under negative pressure and closed under normal pressure. During the filtration and separation stage, negative pressure is applied to keep the tube unobstructed, allowing the liquid to be drawn through the microfiltration membrane 618 while the parasite is retained. When in-situ fixation or sampling is required after separation, the negative pressure is released, and the tube automatically closes to prevent backflow of fixative or air. The collection tube 65 collects all the filtered waste liquid.

[0020] Example 2 Improvements based on Example 1: like Figure 1 , Figure 2 and Figure 4As shown, the sampling base 1 has a long, narrow limiting groove 11. A slider 22 is connected to the bottom surface of the support plate 2, and the slider 22 is slidably disposed within the limiting groove 11. A drive motor 12 is embedded in one side wall of the limiting groove 11. A lead screw 13 is coaxially fixedly connected to the motor shaft of the drive motor 12. The lead screw 13 is threaded through the slider 22. Several ball bearings 21 are embedded in both sides of the bottom surface of the support plate 2. The long, narrow limiting groove 11 provides guidance for the movement of the slider 22 and the entire support plate 2. To prevent deviation or shaking during fish transport, the embedded drive motor 12 directly drives the lead screw 13 to rotate. Through the threaded engagement between the lead screw 13 and the slider 22, the rotational motion of the motor is converted into the linear motion of the slider 22 along the limiting groove 11. This enables the pallet 2 to accurately stop between different work positions, such as the identification work position and the sampling work position. The ball bearings 21 further reduce the frictional resistance between the pallet 2 and the sampling base 1 during movement, making the movement smoother and more fluid.

[0021] like Figure 1 , Figure 2 and Figure 3 As shown, the sampling area identification component also includes an identification housing 3. A light curtain sensor 31, a near-infrared light source 32, and a multispectral camera 33 are all integrated and installed on the inner top surface of the identification housing 3. The near-infrared light source 32 is arranged in a ring shape, and the multispectral camera 33 is installed at the center of the near-infrared light source 32. Notches are provided on both sides of the identification housing 3. The support plate 2 is U-shaped, and when the support plate 2 moves directly under the identification housing 3, the two sides of the support plate 2 engage with the notches in the identification housing 3. The identification housing 3 provides a protective space for the internal light curtain sensor 31, near-infrared light source 32, and multispectral camera 33, effectively preventing external dust... Water vapor pollution or accidental collisions can be prevented, and the interference of external ambient light on multispectral imaging can be shielded or reduced, ensuring that the near-infrared light source 32 is the only dominant light source. This makes the image data collected by the multispectral camera 33 more accurate. The ring-shaped near-infrared light source 32 can uniformly illuminate the surface of the fish being detected from multiple angles, minimizing specular reflection and shadows caused by the curvature of the fish surface and mucus reflection, thereby obtaining high-quality images. When the U-shaped tray 2 carries the fish and moves to the underside of the recognition cover 3, the two arms of the tray 2 can extend into the notch, thereby keeping the internal space of the recognition cover 3 closed and further isolating the interference of external light.

[0022] like Figure 1 , Figure 2 , Figures 5 to 8As shown, a six-axis robotic arm 4 is installed on the sampling base 1, and the installation position of the six-axis robotic arm 4 is located on one side of the moving path of the tray 2. The sampling tube 5 is clamped and fixed at the movable front end of the six-axis robotic arm 4. The six-axis robotic arm 4 can move the piercing needle 53 of the sampling tube 5 to the three-dimensional target coordinate point in the fish body determined by the sampling area recognition component. The six-axis robotic arm 4 can also flexibly adjust the cutting angle of the sampling tube 5 to avoid fish bones or other important organs with the optimal path, so as to achieve true minimally invasive puncture. During the sampling process, the six-axis robotic arm 4 can also drive the sampling tube 5 to perform slight shaking or retraction movements to assist sampling.

[0023] Furthermore, the upper end of the sampling tube 5 is connected to an air inlet connector 54 and a liquid inlet connector 55. A liquid delivery channel 58 is formed inside the sampling tube 5, connecting the liquid inlet connector 55 and the annular liquid guiding cavity 59. Several annularly spaced liquid-passing micro-holes 511 are formed on the inclined surface where the sampling tube 5 and the first variable-diameter tube 51 meet, and all of these micro-holes 511 are connected to the annular liquid guiding cavity 59. An air delivery channel 56 is formed inside the sampling tube 5 and the first variable-diameter tube 51, connecting the air inlet connector 54 and the annular air guiding cavity 57. Several annularly spaced ventilation micro-holes 510 are formed on the inclined surface where the first variable-diameter tube 51 and the second variable-diameter tube 52 meet, and all of these micro-holes 510 are connected to the annular air guiding cavity 57. It should be noted that the air inlet connector 54 is connected to the external sterile gas delivery system, such as CO2 or N2. The pipes are connected, with the inlet connector 55 connected to the external sterile saline delivery pipe. The infusion channel 58, which connects the inlet connector 55 and the annular liquid guiding cavity 59, is opened inside the sampling tube 5, forming a dedicated closed pipeline for liquid delivery. This ensures that the sterile saline can reach the annular liquid guiding cavity 59 without contamination. The liquid accumulated in the annular liquid guiding cavity 59 can be evenly sprayed into the sampling area through the circumferentially distributed liquid-permeable micropores 511, forming a flushing flow field around the piercing needle 53. Similarly, the gas delivery channel 56, which connects the air inlet connector 54 and the annular air guiding cavity 57, is opened inside the sampling tube 5 and the first variable diameter tube body 51, forming a dedicated pipeline for gas delivery. The gas can be evenly sprayed out from the annular air guiding cavity 57 through the opened air-permeable micropores 510, improving the effect of peeling parasite larvae from biological tissue.

[0024] like Figure 1 , Figure 2 , Figures 9 to 15As shown, the negative pressure separation assembly 6 also includes a top cover 62 disposed at the top of the filter tube 63. The top cover 62 is connected to the sampling tube 5 by an infusion tube 7. The top cover 62, the filter tube 63, the microfiltration tube 64, and the collection tube 65 are connected by threads, and the joints of each tube are fitted with sealing rings 8. The infusion tube 7 receives the suspension containing the parasite from the sampling tube 5 and introduces it into the filter tube 63. The top cover 62, the filter tube 63, the microfiltration tube 64, and the collection tube 65 are connected by threads, and the joints of each tube are fitted with sealing rings 8 to ensure that the negative pressure separation assembly 6 has good airtightness when subjected to internal negative pressure, so that the negative pressure can effectively act on the entire filtration path and prevent cross-contamination or pressure loss between the chambers.

[0025] Furthermore, the negative pressure separation component 6 also includes a positioning support plate 61. Vertically arranged support rods 66 are fixedly connected to both ends of the positioning support plate 61 and the sampling base 1. A limiting groove 69 is formed in the middle of the positioning support plate 61, and positioning grooves 610 are provided on both sides of the limiting groove 69. A limiting ring 67 and a positioning block 68 are fixedly connected to the body of the filter tube 63, with the positioning block 68 located below the limiting ring 67. When the body of the filter tube 63 is inserted into the limiting groove 69, the limiting ring 67 is positioned above the limiting groove 69, and the positioning block... The positioning block 68 is placed in the positioning groove 610. When the negative pressure separation component 6 needs to be installed, simply insert the body of the filter tube 63 into the limiting groove 69 of the positioning support plate 61 from top to bottom. The limiting ring 67 will sit above the limiting groove 69 and bear the vertical weight of the entire component. At the same time, the positioning block 68 will fall into the positioning grooves 610 on both sides, which plays the role of circumferential positioning and anti-rotation, preventing the entire component from rotating or swinging when operated or subjected to slight external force, avoiding the vibration or displacement of the pipeline under negative pressure working conditions, and ensuring the stable operation of the separation process.

[0026] Furthermore, an outer ring 614 of the mesh body is movably held in place on the inner wall of the filter tube 63, and a filter screen 615 is connected to the outer ring 614. A liquid-collecting base plate 616 is fixedly connected inside the microfiltration tube 64, and a lower connecting plate 620 is also fixedly connected along the diameter direction inside the microfiltration tube 64. The lower connecting plate 620 is fixedly connected to the liquid-collecting base plate 616. An upper connecting plate 625 is fixedly connected below the outer ring 614, and a slot 626 is provided on the bottom edge of the upper connecting plate 625. The lower connecting plate 620 is inserted into the slot 626 when the filter tube 63 and the microfiltration tube 64 are in the docked state. The microfiltration tube 64 also has... A microfiltration bottom screen 617 is connected to the microfiltration bottom screen 617, which is located on both sides of the lower connecting partition 620. The microfiltration membrane 618 is laid on the microfiltration bottom screen 617. When the filter tube 63 and the microfiltration tube 64 are connected by threads, the upper end of the lower connecting partition 620 will be inserted into the slot 626 of the upper connecting partition 625. This ensures that the internal double-chamber structure of the filter tube 63 and the microfiltration tube 64 can be accurately aligned when they are connected, forming two independent and sealed fluid channels from below the filter screen 615 to the microfiltration membrane 618. The microfiltration bottom screen 617 can support the fragile microfiltration membrane 618 to prevent it from breaking, without affecting the flow of liquid.

[0027] Furthermore, the liquid-collecting base plate 616 is connected to two negative pressure suction pipes 619, and the two negative pressure suction pipes 619 correspond to two independent cavities separated by the lower connecting partition plate 620. An inner cylinder 621 is movably connected inside the negative pressure suction pipe 619. A retaining ring 622 is connected to the body of the inner cylinder 621. An inner groove 627 is opened on the inner wall of the negative pressure suction pipe 619, and the retaining ring 622 is locked in the inner groove 627. A compression spring 623 is also sleeved on the body of the inner cylinder 621. The two ends of the compression spring 623 abut against the bottom surface of the groove between the retaining ring 622 and the inner groove 627, respectively. A drain is opened at the lower end of the inner cylinder 621. The liquid outlet 624 is located at the bottom of the inner cylinder 621, which is initially aligned with the bottom of the negative pressure suction pipe 619. When negative pressure is applied to the collection pipe 65, the negative pressure force overcomes the elastic force of the compression spring 623, causing the inner cylinder 621 to be pulled downwards, thus moving its discharge outlet 624 away from the pipe opening sealing surface and opening the channel, allowing the waste liquid to be suctioned out. When the negative pressure is released, the compression spring 623 pushes the inner cylinder 621 back to its original position, and its bottom surface re-seals the bottom of the negative pressure suction pipe 619. The discharge outlet 624 is hidden or misaligned, and the channel is closed. No additional electrically controlled valves or complex drives are required; the on / off control can be achieved using the working pressure difference.

[0028] Furthermore, when the microfiltration tube 64 is in the negative pressure separation sampling state, its bottom end is connected to the collection tube 65. A negative pressure connector 611 connects the collection tube 65 to the external negative pressure pump, and the negative pressure connector 611 is connected to the upper side wall of the collection tube 65. When the microfiltration tube 64 is in the sampling completed state, its bottom end is connected to a movable bottom cover 612. A stop block 613 is connected inside the movable bottom cover 612, and the stop block 613 abuts against the lower part of the negative pressure suction tube 619 when the microfiltration tube 64 is connected to the movable bottom cover 612. When the microfiltration tube 64 is in the negative pressure separation sampling state, the negative pressure is transmitted to the microfiltration tube 619 through the collection tube 65. At the bottom of tube 64, two negative pressure suction tubes 619 are applied. The negative pressure will draw up the inner cylinder 621 to open the valve port, thereby establishing a continuous negative pressure filtration path. The suspension is filtered, and the insect body is trapped on the microfiltration membrane 618. When filtration is completed and it is time to enter the fixation stage, the bottom end of the microfiltration tube 64 separates from the collection tube 65 and is connected to a movable bottom cover 612. When the microfiltration tube 64 and the movable bottom cover 612 are connected, the set abutment 613 will precisely abut the bottom end of the negative pressure suction tube 619 from below, ensuring that the inner cylinder 621 can be completely reset and sealed, preventing the subsequent injected fixative from leaking out from the bottom.

[0029] Working principle: First, the imported marine fish sample to be inspected is placed on the movable tray 2. The drive motor 12 is started, and the slider 22 is driven by the lead screw 13 to move along the limiting groove 11, thereby smoothly transporting the tray 2 carrying the fish to the area directly below the identification component. The U-shaped design of the tray 2 allows its two sides to fit into the notch of the identification cover 3, forming a relatively closed detection environment. At this time, the light curtain sensor 31 integrated in the identification cover 3 works first, quickly scanning and acquiring the precise three-dimensional contour, size, and real-time position and posture of the fish in space in a non-contact manner. Then, the ring-shaped near-infrared light source 32 emits near-infrared light of a specific wavelength, uniformly illuminating the surface of the fish. The multispectral camera 33 simultaneously captures the multi-band spectral image reflected back from the irradiated area at the center position, identifies suspected parasite-rich areas that are difficult to detect with the naked eye, and fuses the geometric coordinates provided by the light curtain sensor 31 with the image coordinates provided by the multispectral camera 33 to finally calculate a precise aiming point coordinate in three-dimensional space. After identification and positioning are completed, the six-axis robotic arm 4 begins to move. The sampling tube 5, held at its movable front end, moves above the fish body according to the obtained coordinates and is adjusted to the optimal angle, allowing the piercing needle 53 to accurately pierce the target tissue. During the sampling process, sterile saline and sterile gas are delivered into the sampling tube 5 through the liquid inlet connector 55 and the air inlet connector 54, respectively. The saline is delivered to the annular liquid guiding chamber 59 through the infusion channel 58 and is evenly sprayed into the sampling area through the annularly distributed liquid-perforating micropores 511, which serves to flush and suspend parasites. At the same time, sterile gas is delivered to the annular air guiding chamber 57 through the air delivery channel 56 and is ejected through the air-perforating micropores 510, which helps to disturb the tissue gaps, prevent blockage, and create a gas-liquid synergistic peeling environment. Under negative pressure, the mixed suspension containing the detached parasite is drawn out through the sampling tube 5 and transported to the negative pressure separation component 6 via the infusion tube 7. The suspension first enters the filter tube 63, where the internal filter screen 615 intercepts larger tissue fragments and other impurities for preliminary purification. Subsequently, the liquid flows into the two independent cavities of the lower microfiltration tube 64. Each cavity has a microfiltration membrane 618 supported by a microfiltration bottom screen 617 at the bottom, used to trap tiny parasite larvae. At this time, the device is in negative pressure separation sampling state, with the bottom end of the microfiltration tube 64 connected to the collection tube 65, and an external negative pressure pump applying suction through the negative pressure connector 611. This negative pressure acts on the negative pressure suction tube 619, overcoming the elastic force of the compression spring 623 to lift the inner cylinder 621, opening its drain port 624, allowing the filtrate to be drawn away and discharged into the collection tube 65, while the parasite remains on the surface of the microfiltration membrane 618. After filtration, the device switches states. The negative pressure is released, and the compression spring 623 pushes the inner cylinder 621 back to its original position, closing the bottom of the negative pressure suction tube 619. The collection tube 65 is unscrewed, and the movable bottom cover 612 is installed. The internal stop block 613 further presses against the bottom of the negative pressure suction tube 619 to ensure a seal. At this point, the two independent cavities become sealed reaction chambers, allowing for the injection of different fixatives to fix the intercepted parasite samples in situ, achieving multiple samples from a single sample.

[0030] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0031] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A sampling and detection device for pathogenic parasites in imported marine fish, comprising a sampling base (1), characterized in that: The sampling base (1) is provided with a connected sampling tube (5) and a negative pressure separation component (6). The sampling base (1) is movably provided with a tray (2). A sampling area identification component is provided directly above the moving path of the tray (2). The sampling area identification component includes a light curtain sensor (31), a near-infrared light source (32), and a multispectral camera (33). The sampling tube (5) is provided with a first variable diameter tube body (51), a second variable diameter tube body (52) and a piercing needle (53) in sequence. An annular air guiding cavity (57) is opened at the docking part of the sampling tube (5) and the first variable diameter tube body (51), and the air in the annular air guiding cavity (57) flows into the sampling area. An annular liquid guiding cavity (59) is opened at the docking part of the first variable diameter tube body (51) and the second variable diameter tube body (52), and the liquid in the annular liquid guiding cavity (59) flows into the sampling area. The negative pressure separation component (6) includes a filter tube (63), a microfiltration tube (64) and a liquid collection tube (65) arranged sequentially from top to bottom. The filter tube (63) is provided with a filter screen (615). The microfiltration tube (64) is provided with two independent cavities, and each cavity is provided with a microfiltration membrane (618). The bottom end of each cavity of the microfiltration tube (64) is connected to a negative pressure liquid extraction tube (619). The negative pressure liquid extraction tube (619) is in a closed state when it is under negative pressure and in a closed state when it is under normal pressure.

2. The sampling and detection device for pathogenic parasites in imported marine fish according to claim 1, characterized in that: The sampling base (1) has a long, narrow limiting groove (11) on its body. The bottom surface of the support plate (2) is connected to a slider (22), and the slider (22) is slidably disposed in the limiting groove (11). A drive motor (12) is embedded in one side wall of the limiting groove (11). The motor shaft of the drive motor (12) is coaxially fixedly connected to a lead screw (13). The screw (13) is threaded through the slider (22). Several balls (21) are embedded in both sides of the bottom surface of the support plate (2).

3. The sampling and detection device for pathogenic parasites in imported marine fish according to claim 1, characterized in that: The sampling area identification component also includes an identification cover (3). The light curtain sensor (31), near-infrared light source (32) and multispectral camera (33) are all integrated and installed on the inner top surface of the identification cover (3). The near-infrared light source (32) is arranged in a ring shape, and the installation position of the multispectral camera (33) is located at the center of the near-infrared light source (32). There are notches on both sides of the identification cover (3). The plate (2) is U-shaped, and when the plate (2) moves to the bottom of the identification cover (3), the two sides of the plate (2) engage with the notches in the body of the identification cover (3).

4. The sampling and detection device for pathogenic parasites in imported marine fish according to claim 1, characterized in that: A six-axis robotic arm (4) is installed on the sampling base (1), and the installation position of the six-axis robotic arm (4) is located on one side of the moving path of the pallet (2). The sampling tube (5) is clamped and fixed at the movable front end of the six-axis robotic arm (4).

5. The sampling and detection device for pathogenic parasites in imported marine fish according to claim 1, characterized in that: The upper end of the sampling tube (5) is connected to an air inlet connector (54) and a liquid inlet connector (55). A liquid delivery channel (58) is opened inside the sampling tube (5) connecting the liquid inlet connector (55) and the annular liquid guiding cavity (59). A plurality of annular liquid-passing microholes (511) are opened on the inclined surface of the sampling tube (5) and the first variable diameter tube (51), and a plurality of the liquid-passing microholes (511) are connected to the annular liquid guiding cavity. The cavity (59) is connected. The sampling tube (5) and the first variable diameter tube body (51) are provided with an air supply channel (56) connecting the air inlet connector (54) and the annular air guide cavity (57). The first variable diameter tube body (51) and the second variable diameter tube body (52) are provided with a number of annularly spaced ventilation microholes (510) at the joint slope. All of the ventilation microholes (510) are connected to the annular air guide cavity (57).

6. The sampling and detection device for pathogenic parasites in imported marine fish according to claim 1, characterized in that: The negative pressure separation assembly (6) also includes an upper cover (62) set at the top of the filter tube (63). The upper cover (62) is connected to the sampling tube (5) by an infusion tube (7). The upper cover (62), filter tube (63), microfiltration tube (64) and collection tube (65) are connected by threads, and the connection parts of each tube are fitted with sealing rings (8).

7. The sampling and detection device for pathogenic parasites in imported marine fish according to claim 6, characterized in that: The negative pressure separation component (6) also includes a positioning support plate (61). The two ends of the positioning support plate (61) are fixedly connected to the sampling base (1) with vertically arranged support rods (66). A limiting groove (69) is opened in the middle part of the plate body of the positioning support plate (61). Positioning grooves (610) are provided on both sides of the limiting groove (69). A limiting ring (67) and a positioning block (68) are fixedly connected to the tube body of the filter tube (63). The positioning block (68) is located below the limiting ring (67). When the tube body of the filter tube (63) is inserted into the limiting groove (69), the limiting ring (67) is locked above the limiting groove (69), and the positioning block (68) is locked in the positioning groove (610).

8. The sampling and detection device for pathogenic parasites in imported marine fish according to claim 7, characterized in that: The inner wall of the filter tube (63) is movably fitted with a mesh outer ring (614), and the filter screen (615) is connected to the mesh outer ring (614). A liquid-collecting base plate (616) is fixedly connected inside the microfiltration tube (64). A lower connecting partition plate (620) is also fixedly connected along the diameter direction inside the microfiltration tube (64). The lower connecting partition plate (620) is fixedly connected to the liquid-collecting base plate (616). An upper connecting partition plate is fixedly connected below the mesh outer ring (614). The upper docking partition (625) has a slot (626) on its bottom edge. The lower docking partition (620) is inserted into the slot (626) when the filter tube (63) and the microfiltration tube (64) are docked. The microfiltration tube (64) is also connected to a microfiltration bottom screen (617). The microfiltration bottom screen (617) is located on both sides of the lower docking partition (620). The microfiltration membrane (618) is laid on the microfiltration bottom screen (617).

9. The sampling and detection device for pathogenic parasites in imported marine fish according to claim 8, characterized in that: The liquid-collecting base plate (616) is connected to two negative pressure liquid-drawing pipes (619), and the two negative pressure liquid-drawing pipes (619) correspond to two independent cavities separated by the lower connecting partition plate (620). An inner cylinder (621) is movably connected inside the negative pressure liquid-drawing pipe (619), and a retaining ring (622) is connected to the body of the inner cylinder (621). An inner groove (627) is opened on the inner wall of the negative pressure liquid-drawing pipe (619), and the retaining ring (622) 622) is placed in the concave groove (627). The inner cylinder (621) is also fitted with a compression spring (623). The two ends of the compression spring (623) are respectively abutted between the retaining ring (622) and the bottom surface of the concave groove (627). The lower end of the inner cylinder (621) is provided with a drain port (624). The bottom surface of the inner cylinder (621) is in the same plane as the bottom end of the negative pressure suction pipe (619) in the initial state.

10. The sampling and detection device for pathogenic parasites in imported marine fish according to claim 9, characterized in that: When the microfiltration tube (64) is in the negative pressure separation sampling state, its bottom end is connected to the collection tube (65). The collection tube (65) is connected to the external negative pressure pump by a negative pressure connector (611), and the negative pressure connector (611) is connected to the upper side wall of the collection tube (65). When the microfiltration tube (64) is in the sampling completed state, its bottom end is connected to a movable bottom cover (612). The movable bottom cover (612) is connected to a stop block (613), and the stop block (613) is pressed against the bottom of the negative pressure suction tube (619) when the microfiltration tube (64) and the movable bottom cover (612) are connected.

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