Compound microbial agent, preparation method, application and system

Abstract

The invention relates to a compound microbial agent, a preparation method, application and a system. The compound microbial agent is prepared from the following raw materials in parts by weight: 100 parts of a fermented and decomposed compound organic carrier; 0.5-5 parts by weight of a functional microbial flora; 5 to 15 parts by weight of a functional conditioner; the composite organic carrier is composed of cotton stalk decomposed substances, agricultural processing residues and livestock and poultry manure; the functional microbial flora comprises bacillus, actinomycetes and saccharomycetes. According to the invention, the resource utilization of agricultural wastes is realized, the cost is reduced, more importantly, the carriers are rich in organic matters and various nutrients, and an excellent living environment and continuous nutrition supply are provided for functional flora. A micro-ecological system with complementary functions and synergistic interaction is constructed on the level of flora, the microbial agent is fundamentally different from a single-strain microbial agent, and a material basis is provided for solving the complex problems of saline-alkali coexistence and weak microbial activity of saline-alkali soil.

Description

Technical Field

[0001] This invention relates to the field of bio-fertilizer technology, and in particular to a compound microbial agent, its preparation method, application, and system. Background Technology

[0002] The current saline-alkali environment in southern Xinjiang is harsh, with soil salinity as high as 3%-5% and pH values ​​ranging from 8.5 to 10.0. Common bacterial strains have a colonization rate of less than 30% in this environment, and their salt-solubilizing and growth-promoting functions are inhibited due to high osmotic pressure, making it difficult to sustain the improvement effect of microbial fertilizers. Furthermore, existing microbial fertilizer carriers mostly use single bacterial strains, making it difficult to simultaneously meet the multiple requirements of "salt reduction, quality improvement, and disease resistance," and failing to address the combined problems of poor soil quality and poor crop resistance in saline-alkali land. At the same time, the widely used bacterial strains are mostly from other regions, exhibiting low activity and poor efficacy in Xinjiang's extreme environment, poor synergy among compound microbial communities, and high costs for large-scale propagation.

[0003] A large amount of agricultural waste, such as cotton stalks, awaits processing in the area. Because the straw cellulose is coarse and hard, the composting and fermentation cycle is long, and secondary fermentation is prone to occur, leading to nutrient loss from the carrier. At the same time, the synergy between the carrier, functional bacterial strains, and conditioners is poor; some carrier components can inhibit bacterial activity. The compatibility problem among the carrier, bacteria, and conditioners urgently needs to be solved. Furthermore, even if good bacterial strains are selected, traditional fermentation processes result in low yields and high costs, making large-scale, low-cost production difficult.

[0004] Traditional high-temperature granulation results in a high inactivation rate of bacterial strains, failing to meet the "high viable bacteria count" requirement for microbial fertilizers. Although high-tower granulation technology can solve this problem, the compatibility between the characteristics of saline-alkali soils and microbial fertilizers is unclear. Aksu saline-alkali soils are predominantly "sulfate-chloride type," and existing research lacks data on the impact of this type of saline-alkali soil on novel bio-organic fertilizers. Furthermore, the compatibility parameters between soils with different salinity levels and microbial fertilizers are unclear, hindering the precise application of organic fertilizers.

[0005] Therefore, it is evident that the existing compound microbial agents still have inconveniences and defects in terms of methods, manufacturing methods, processing methods, and applications, and urgently require further improvement. To address the problems of compound microbial agents, relevant manufacturers have spared no effort in seeking solutions, but for a long time, no suitable design has been developed. Furthermore, there are no appropriate methods, manufacturing methods, processing methods, or structures for general methods, manufacturing methods, processing methods, or compound microbial agents to solve the aforementioned problems. This is clearly a problem that relevant industries urgently need to solve.

[0006] In view of the shortcomings of existing compound microbial agents, the inventor, based on years of practical experience and professional knowledge in the design and manufacture of such products, and in conjunction with theoretical application, actively researched and innovated to create a new compound microbial agent that could improve upon existing compound microbial agents and make them more practical. Through continuous research, design, and repeated trials and improvements, this invention, with proven practical value, was finally created. Summary of the Invention

[0007] The main objective of this invention is to overcome the shortcomings of existing compound microbial agents and provide a new type of compound microbial agent. The technical problem to be solved is that this agent not only achieves resource utilization of agricultural waste and reduces costs, but more importantly, these carriers themselves are rich in organic matter and various nutrients, providing an excellent living environment and continuous nutrient supply for the functional microbial community. At the microbial community level, a functionally complementary and synergistic micro-ecosystem is constructed, fundamentally different from single-species agents. This provides a material basis for solving the complex problem of "coexistence of salinity and alkali and weak microbial activity" in saline-alkali land, making it more suitable for practical application and possessing industrial utilization value.

[0008] Another objective of this invention is to provide a method for preparing composite microbial inoculants. The technical problem addressed is that this method not only enables the resource utilization of agricultural waste and reduces costs, but more importantly, the carriers themselves are rich in organic matter and various nutrients, providing an excellent living environment and continuous nutrient supply for the functional microbial communities. This constructs a functionally complementary and synergistically effective micro-ecosystem at the microbial community level, fundamentally differentiating it from single-species inoculants. This provides a material basis for solving the complex problem of "coexistence of salinity and alkali and weak microbial activity" in saline-alkali land, making it more suitable for practical application.

[0009] Compared with the prior art, the present invention has significant advantages and beneficial effects. As can be seen from the above technical solution, in order to achieve the aforementioned objectives, the main technical contents of the present invention are as follows:

[0010] On the one hand, a compound microbial agent for improving saline-alkali land is provided, which is prepared from raw materials comprising the following parts by weight:

[0011] Fermented and decomposed composite organic carrier: 100 parts by weight;

[0012] Functional microbial flora: 0.5-5 parts by weight;

[0013] Functional conditioning agent: 5-15 parts by weight;

[0014] The composite organic carrier is composed of decomposed cotton stalks, agricultural processing residues, and livestock and poultry manure.

[0015] The functional microbial community includes Bacillus, Actinomycetes, and yeast.

[0016] In one optional embodiment, the mass ratio of decomposed cotton stalks, agricultural processing residues and livestock and poultry manure in the composite organic carrier is (3-5):(1-3):(4-6).

[0017] In one alternative embodiment, the functional conditioner includes at least one of humic acid, seaweed extract, and nano-silicon fertilizer.

[0018] In an alternative embodiment, the functional microbial community is obtained through gradient acclimatization and screening in a culture medium with a salt concentration of 3%-8% and a pH of 8.5-10.0.

[0019] In one alternative embodiment, the microbial agent is prepared by a process including low-temperature granulation, wherein the temperature of the low-temperature granulation is not higher than -20°C.

[0020] On the other hand, a method for preparing the composite microbial agent according to any one of the above is provided, characterized by comprising the following steps:

[0021] (1) Preparation of composite carrier: Cotton stalks, agricultural processing residues and livestock and poultry manure are mixed in proportion and subjected to high-temperature aerobic fermentation to obtain a decomposed composite organic carrier.

[0022] (2) Microbial community expansion: The functional microbial community is expanded by liquid deep fermentation in a composite culture medium;

[0023] (3) Mixing and granulation: The composite organic carrier obtained in step (1), the bacterial solution obtained in step (2) and the functional conditioner are mixed evenly and granulated using low-temperature granulation technology.

[0024] In one alternative embodiment, in step (1), the temperature of the high-temperature aerobic fermentation is 55-65°C, and the fermentation cycle is 15-20 days.

[0025] In one optional embodiment, in step (2), the liquid submerged fermentation propagation is carried out in a fermenter, and the fermentation conditions satisfy at least one of the following parameters:

[0026] Fermentation temperature should be controlled at 30-37℃;

[0027] The pH of the fermentation broth was maintained between 6.5 and 7.5;

[0028] The stirring speed should be controlled at 150-250 rpm;

[0029] Ventilation volume was maintained at 0.8-1.2 vvm;

[0030] Dissolved oxygen levels should be maintained at 30%-50% saturation.

[0031] On the other hand, the application of the aforementioned compound microbial agent in improving sulfate-chloride type saline-alkali land is provided.

[0032] On the other hand, a saline-alkali land improvement system is provided, including:

[0033] The compound microbial agent as described in any one of the above statements;

[0034] And, a drip irrigation system for applying the bacterial agent.

[0035] As described above, this invention relates to a composite microbial inoculant, its preparation method, application, and system, prepared from the following raw materials in parts by weight: a fermented and decomposed composite organic carrier: 100 parts by weight; a functional microbial community: 0.5-5 parts by weight; and a functional conditioner: 5-15 parts by weight. The composite organic carrier consists of decomposed cotton stalks, agricultural processing residues, and livestock and poultry manure. The functional microbial community includes Bacillus, Actinomycetes, and yeast. This invention not only realizes the resource utilization of agricultural waste and reduces costs, but more importantly, these carriers themselves are rich in organic matter and various nutrients, providing an excellent living environment and continuous nutrient supply for the functional microbial community. It constructs a functionally complementary and synergistically effective micro-ecosystem at the microbial community level, fundamentally different from single-species inoculants, and provides a material basis for solving the complex problem of "coexistence of salinity and alkali and weak microbial activity" in saline-alkali land.

[0036] By employing the above technical solution, the composite microbial agent of the present invention has at least the following advantages:

[0037] This invention specifies the source and type of the core components. Using a composite carrier derived from a specific source—"decomposed cotton stalks, agricultural processing residues, and livestock manure"—not only achieves resource utilization of agricultural waste and reduces costs, but more importantly, these carriers themselves are rich in organic matter and various nutrients, providing an excellent living environment and continuous nutrient supply for the functional microbial community. Simultaneously, the specific combination of "Bacillus, Actinomycetes, and Yeast" is defined for the functional microbial community. This utilizes the different functions of Bacillus (stress resistance and growth promotion), Actinomycetes (decomposition and disease resistance), and Yeast (hormone production and mutualistic symbiosis), constructing a functionally complementary and synergistic micro-ecosystem at the microbial community level. This fundamentally distinguishes it from single-species inoculants and provides a material basis for solving the complex problem of "coexistence of salinity and alkali and weak microbial activity" in saline-alkali land.

[0038] In summary, the unique composite microbial agent of this invention utilizes a composite carrier derived from a specific source: "decomposed cotton stalks, agricultural processing residues, and livestock manure." This not only achieves the resource utilization of agricultural waste and reduces costs, but more importantly, these carriers themselves are rich in organic matter and various nutrients, providing an excellent living environment and continuous nutrient supply for the functional microbial community. Furthermore, the specific combination of "Bacillus, Actinomycetes, and Yeast" within the defined functional microbial community leverages the stress-resistance and growth-promoting properties of Bacillus, the decomposition and disease resistance of Actinomycetes, and the hormone production and mutualistic symbiosis of Yeast. This constructs a functionally complementary and synergistic micro-ecosystem at the microbial community level, fundamentally distinguishing it from single-species agents and providing a material basis for solving the complex problem of "coexistence of salinity and alkali and weak microbial activity" in saline-alkali land. It possesses numerous advantages and practical value, and is truly innovative as no similar design has been publicly disclosed or used in the same category (method, manufacturing method, processing method). It represents a significant improvement in both method, manufacturing method, and processing method, as well as in function, demonstrating substantial technological advancement and producing user-friendly and practical effects. Furthermore, it offers enhanced efficacy compared to existing compound microbial agents, making it more suitable for practical application and possessing broad industrial value. It is indeed a novel, progressive, and practical new design.

[0039] The above description is merely an overview of the technical solution of the present invention. In order to better understand the technical means of the present invention and to implement it in accordance with the contents of the specification, the preferred embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0040] The specific details (method, manufacturing method, processing method) and structure of the present invention are given in detail in the following embodiments and accompanying drawings. Attached Figure Description

[0041] Appendix Figure 1 A schematic diagram of the process for preparing a compound microbial agent for improving saline-alkali land provided in an embodiment of the present invention. Detailed Implementation

[0042] To further illustrate the technical means and effects adopted by the present invention to achieve the intended purpose, the following detailed description, in conjunction with the accompanying drawings and preferred embodiments, details the specific implementation methods, manufacturing methods, processing methods, steps, structures, features, and effects of the compound microbial agent for saline-alkali land improvement proposed according to the present invention.

[0043] On one hand, embodiments of the present invention provide a compound microbial agent for improving saline-alkali land, which is prepared from raw materials comprising the following parts by weight:

[0044] Fermented and decomposed composite organic carrier: 100 parts by weight;

[0045] Functional microbial flora: 0.5-5 parts by weight;

[0046] Functional conditioning agent: 5-15 parts by weight;

[0047] The composite organic carrier consists of decomposed cotton stalks, agricultural processing residues, and livestock and poultry manure;

[0048] Functional microbial communities include Bacillus, Actinomycetes, and yeasts.

[0049] This invention specifies the source and type of the core components. Using a composite carrier derived from a specific source—"decomposed cotton stalks, agricultural processing residues, and livestock manure"—not only achieves resource utilization of agricultural waste and reduces costs, but more importantly, these carriers themselves are rich in organic matter and various nutrients, providing an excellent living environment and continuous nutrient supply for the functional microbial community. Simultaneously, the specific combination of "Bacillus, Actinomycetes, and Yeast" is defined for the functional microbial community. This utilizes the different functions of Bacillus (stress resistance and growth promotion), Actinomycetes (decomposition and disease resistance), and Yeast (hormone production and mutualistic symbiosis), constructing a functionally complementary and synergistic micro-ecosystem at the microbial community level. This fundamentally distinguishes it from single-species inoculants and provides a material basis for solving the complex problem of "coexistence of salinity and alkali and weak microbial activity" in saline-alkali land.

[0050] In one optional embodiment, the mass ratio of decomposed cotton stalks, agricultural processing residues and livestock and poultry manure in the composite organic carrier is (3-5):(1-3):(4-6).

[0051] This invention further defines the core ratio of the composite carrier. This specific ratio is an optimized golden ratio that achieves the best composting effect and carrier performance. It ensures that the carrier has a suitable carbon-nitrogen ratio, enabling the high-temperature aerobic fermentation process to start quickly and thoroughly decompose (the cycle can be shortened to 18 days), avoiding the risk of root burn from "secondary fermentation". At the same time, the carrier with this ratio has a loose physical structure, good water retention and air permeability, stable chemical properties, and a moderate pH, which is most conducive to the adsorption, survival, and subsequent reproduction of functional bacteria, serving as a bridge connecting waste resource utilization and the production of high-efficiency microbial agents.

[0052] In one alternative embodiment, the functional conditioner includes at least one of humic acid, seaweed extract, and nano-silicon fertilizer.

[0053] The humic acid provided in this invention can chelate salt ions and stimulate crop growth; seaweed extract is rich in natural active substances and can enhance crop stress resistance; nano-silicon fertilizer can strengthen crop cell walls and reduce salt uptake. These, in synergy with functional microorganisms and organic carriers, constitute a three-in-one improvement system of "physical improvement (carrier) - chemical conditioning (conditioner) - bioremediation (microorganisms)," achieving simultaneous action on saline-alkali soils from different levels.

[0054] In an alternative embodiment, the functional microbial community is obtained through gradient acclimatization and screening in a culture medium with a salt concentration of 3%-8% and a pH of 8.5-10.0.

[0055] It should be noted that the strains are not ordinary commercial strains, but rather "special forces" selected through a targeted gradient domestication method—an artificial evolutionary process—specifically designed for the extreme "high salinity, high pH" environment of Xinjiang's saline-alkali land. Strains that survive and remain active under these conditions possess a natural and extremely strong adaptability and colonization ability to the target saline-alkali environment. This solves the core bottleneck of introduced strains' "acclimatization problems" and low colonization rate (less than 30%), ensuring that the microbial agent can truly survive and exert its effects after being applied to the soil.

[0056] In an alternative embodiment, the microbial agent is prepared by a process including low-temperature granulation, wherein the temperature of low-temperature granulation is not higher than -20°C.

[0057] Understandably, traditional fertilizer granulation is mostly a high-temperature process, which kills a large number of temperature-sensitive functional bacteria. This invention uses a low-temperature granulation technology at no higher than -20℃ to ensure that the final product has a stable viable bacteria count of 2×10⁻⁶. 8 The decisive step is achieving a concentration of CFU / g or higher. This technical feature directly resolves the inherent contradiction between "granulation" and "vitality preservation" in the industrialization of bio-fertilizers, and is the key technical guarantee for this invention to move from the laboratory to large-scale production.

[0058] Please see Figure 1 Furthermore, a method for preparing any of the above-mentioned compound microbial agents is provided, comprising the following steps:

[0059] S1. Preparation of composite carrier: Cotton stalks, agricultural processing residues and livestock and poultry manure are mixed in proportion and subjected to high-temperature aerobic fermentation to obtain a decomposed composite organic carrier.

[0060] S2. Microbial community expansion: Functional microbial communities are expanded by liquid deep fermentation in a composite culture medium;

[0061] S3. Mixing and granulation: The composite organic carrier obtained in step S1, the bacterial solution obtained in step S2, and the functional conditioner are mixed evenly and granulated using low-temperature granulation technology.

[0062] This invention systematically integrates three key process steps: directional composting, efficient propagation, and low-temperature molding. Each step is optimized for the performance of the final product (high viable count, carrier adaptability, and environmental friendliness), forming a complete, efficient, and industrially scalable production chain. This method ensures that the activity and adaptability of functional microbiota are preserved and enhanced to the greatest extent possible throughout the entire process from raw materials to finished product.

[0063] In one alternative embodiment, in step (1), the temperature of the high-temperature aerobic fermentation is 55-65°C, and the fermentation cycle is 15-20 days.

[0064] It should be noted that this temperature range is where thermophilic microorganisms are most active, enabling them to rapidly decompose organic matter, kill pathogens and weed seeds, achieving efficient and harmless processing. Controlling the cycle to 15-20 days (preferably 18 days) is an efficiency improvement achieved through process optimization, far shorter than the traditional composting cycle of over 30 days. This is significant for reducing production costs and increasing production capacity.

[0065] In an optional embodiment, in step (2), the liquid submerged fermentation propagation is carried out in a fermenter, and the fermentation conditions satisfy at least one of the following parameters:

[0066] Fermentation temperature should be controlled at 30-37℃;

[0067] The pH of the fermentation broth was maintained between 6.5 and 7.5;

[0068] The stirring speed should be controlled at 150-250 rpm;

[0069] Ventilation volume was maintained at 0.8-1.2 vvm;

[0070] Dissolved oxygen levels should be maintained at 30%-50% saturation.

[0071] This invention clearly defines the process window for achieving efficient microbial colony propagation by specifically limiting the ranges of five core parameters: temperature, pH, stirring rate, aeration rate, and dissolved oxygen. These parameter ranges are optimal intervals obtained through extensive experimental optimization: within this temperature range, the growth and metabolism of the functional microbial colony are most vigorous; this pH range is most suitable for cell growth and maintaining the stability of the fermentation system; the specific combination of stirring rate and aeration rate ensures sufficient oxygen supply without damaging the cells due to excessive shear force; and controlling the dissolved oxygen level at 30%-50% saturation ensures the aerobic respiration efficiency of the cells. The synergistic control of these specific parameters achieves high cell density (viable cell count ≥ 2 × 10⁻⁶). 8 The key to this approach (CFU / g) and shortening the fermentation cycle is to ensure that the technical solution is sufficiently clear and feasible.

[0072] In another aspect, embodiments of the present invention provide the application of compound microbial agents in improving sulfate-chloride type saline-alkali land.

[0073] In another aspect, embodiments of the present invention provide a saline-alkali land improvement system, comprising:

[0074] The compound microbial agent of any of the above; and a drip irrigation system for applying the agent.

[0075] The following will further explain and describe the compound microbial agent for improving saline-alkali land provided by the embodiments of the present invention through optional examples.

[0076] Example 1

[0077] A compound microbial agent for improving saline-alkali land is prepared from the following raw materials in parts by weight: fermented and decomposed compound organic carrier: 100 parts by weight; functional microbial community: 0.5 parts by weight; functional conditioner: 5 parts by weight; the compound organic carrier is composed of decomposed cotton stalks, agricultural processing residues and livestock and poultry manure; the functional microbial community includes Bacillus, Actinomycetes and yeast.

[0078] Example 2

[0079] A compound microbial agent for improving saline-alkali land is prepared from the following raw materials in parts by weight: fermented and decomposed compound organic carrier: 100 parts by weight; functional microbial community: 5 parts by weight; functional conditioner: 15 parts by weight; the compound organic carrier is composed of decomposed cotton stalks, agricultural processing residues and livestock and poultry manure; the functional microbial community includes Bacillus, Actinomycetes and yeast.

[0080] Example 3

[0081] A compound microbial agent for improving saline-alkali land is prepared from the following raw materials in parts by weight: fermented and decomposed compound organic carrier: 100 parts by weight; functional microbial community: 3 parts by weight; functional conditioner: 10 parts by weight; the compound organic carrier is composed of decomposed cotton stalks, agricultural processing residues and livestock and poultry manure; the functional microbial community includes Bacillus, Actinomycetes and yeast.

[0082] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the methods and techniques disclosed above without departing from the scope of the present invention to create equivalent embodiments. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A compound microbial agent for improving saline-alkali land, characterized in that, It is prepared from the following raw materials in parts by weight: Fermented and decomposed composite organic carrier: 100 parts by weight; Functional microbial flora: 0.5-5 parts by weight; Functional conditioning agent: 5-15 parts by weight; The composite organic carrier is composed of decomposed cotton stalks, agricultural processing residues, and livestock and poultry manure. The functional microbial community includes Bacillus, Actinomycetes, and yeast.

2. The compound microbial agent as described in claim 1, characterized in that, In the composite organic carrier, the mass ratio of decomposed cotton stalks, agricultural processing residues and livestock and poultry manure is (3-5):(1-3):(4-6).

3. The composite microbial agent as described in claim 1 or 2, characterized in that, The functional conditioning agent includes at least one of humic acid, seaweed extract, and nano-silicon fertilizer.

4. The compound microbial agent as described in claim 1, characterized in that, The functional microbial community was obtained through gradient domestication and screening in a culture medium with a salt concentration of 3%-8% and a pH of 8.5-10.

0.

5. The compound microbial agent as described in claim 1, characterized in that, The bacterial agent is prepared by a process including low-temperature granulation, wherein the temperature of the low-temperature granulation is not higher than -20°C.

6. A method for preparing the composite microbial agent as described in any one of claims 1-5, characterized in that, Includes the following steps: (1) Preparation of composite carrier: Cotton stalks, agricultural processing residues and livestock and poultry manure are mixed in proportion and subjected to high-temperature aerobic fermentation to obtain a decomposed composite organic carrier. (2) Microbial community expansion: The functional microbial community is expanded by liquid deep fermentation in a composite culture medium; (3) Mixing and granulation: The composite organic carrier obtained in step (1), the bacterial solution obtained in step (2) and the functional conditioner are mixed evenly and granulated using low-temperature granulation technology.

7. The method as described in claim 6, characterized in that, In step (1), the temperature of the high-temperature aerobic fermentation is 55-65℃, and the fermentation cycle is 15-20 days.

8. The method as described in claim 6, characterized in that, In step (2), the liquid submerged fermentation propagation is carried out in a fermenter, and the fermentation conditions meet at least one of the following parameters: Fermentation temperature should be controlled at 30-37℃; The pH of the fermentation broth was maintained between 6.5 and 7.5; The stirring speed should be controlled at 150-250 rpm; Ventilation volume was maintained at 0.8-1.2 vvm; Dissolved oxygen levels should be maintained at 30%-50% saturation.

9. The application of the compound microbial agent as described in any one of claims 1-5 in improving sulfate-chloride type saline-alkali land.

10. A saline-alkali land improvement system, characterized in that, include: The compound microbial agent as described in any one of claims 1-5; And, a drip irrigation system for applying the bacterial agent.

Images