Massage cream composition for repairing hair, relieving scalp and resisting aging, preparation method, application and product

Abstract

The invention provides a massage cream composition for repairing hair, relieving scalp and resisting aging as well as a preparation method, application and a product thereof, and relates to the technical field of cosmetics. The massage cream composition provided by the invention is prepared from the following components in parts by weight: 3 to 8 parts of cacumen biotae extract, 0.5 to 1.5 parts of mint leaf extract, 0.5 to 1.5 parts of rosemary leaf oil, 0.5 to 1.5 parts of lavender oil, 0.5 to 1.5 parts of basil oil, 0.5 to 1.5 parts of bergamot fruit oil, 0.5 to 1.5 parts of eucalyptus globulus leaf oil, 0.5 to 1.5 parts of agilawood extract, 0.5 to 1.5 parts of ginger root oil, 1.2 to 3 parts of bisabolol and 0.5 to 1.5 parts of tetrahydromethylpyrimidine carboxylic acid. All the components cooperate with one another, and the effects of relieving scalp, improving hair follicle vitality, promoting hair growth, repairing hair, repairing scalp, maintaining scalp barrier, preserving moisture, tightening scalp, resisting wrinkles and resisting aging can be achieved.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a massage cream composition for repairing hair, soothing the scalp, and anti-aging, as well as its preparation method, application, and products. Background Technology

[0002] Hair, referring to the hair growing on the head, contains no nerves or blood vessels but does contain cells. As part of the body's structure, hair plays a direct protective role for the scalp. Thick, shiny hair represents health; however, many people suffer from dry, frizzy hair, severe hair loss, and itchy scalps. This not only leads to sub-health conditions like baldness but also manifests as redness, rashes, pimples, or excessive keratinization and scalp inflammation. The scalp, as the "soil" for hair, directly determines the vitality of hair growth. Insufficient scalp relaxation easily causes itching, redness, and sensitivity, while a loose scalp leads to decreased hair follicle support and poor blood circulation, further exacerbating hair loss and thinning. Therefore, the research and development of scalp care product ingredients with multiple functions, including improving hair follicle vitality, promoting hair growth, repairing hair growth, repairing the scalp, moisturizing, firming, and anti-aging, is a hot topic.

[0003] Traditional hair care products are mostly targeted at the hair itself, using large amounts of silicone oil and cationic surfactants to quickly improve hair condition, making it smooth and shiny. While this superficially solves some problems, these quick fixes don't address the scalp's condition and therefore don't address the root cause of hair problems. While using herbal extracts in hair care can improve scalp health, promote metabolism, improve blood circulation in the cerebral cortex, and strengthen hair, improper use of these ingredients can actually increase the risk of hair loss, damage hair follicles, and increase scalp sensitivity.

[0004] Chinese invention patent CN121102110A discloses a herbal composition for preventing hair loss and promoting hair growth, its preparation method, and its application. The herbal composition includes herbal extracts obtained through extraction from a mixture of herbs. The herbal mixture includes ginseng, black Ganoderma lucidum, prepared Polygonum multiflorum, Rehmannia glutinosa, Ligusticum chuanxiong, Angelica sinensis, Gallnut, Platycladus orientalis leaves, Zingiber officinale, Salvia miltiorrhiza, Eclipta prostrata, Ligustrum lucidum, Camellia sinensis seeds, Paeonia suffruticosa bark, Sapindus mukorossi, Gleditsia sinensis, Artemisia argyi leaves, mulberry leaves, Senecio scandens, Lonicera japonica, Psoralea corylifolia, Phyllanthus emblica, Sophora flavescens, Cnidium monnieri, Polygonum hydropiper, Lysimachia christinae, aloe vera juice, elm bark, agarwood, Dictamnus dasycarpus bark, and peppermint leaves. This herbal composition for preventing hair loss and promoting hair growth, through the action of various components in the herbal extracts, achieves deep cleansing and nourishing of the scalp, promotes scalp blood circulation, activates hair follicles, strengthens hair roots, and has the effects of preventing hair loss and promoting hair growth, protecting and darkening hair, controlling oil and moisturizing, and removing dandruff and relieving itching.

[0005] Chinese invention patent CN109966181A discloses a herbal hair care essential oil prepared from the following components: wheat germ oil, sweet almond oil, rosemary leaf oil, geranium flower oil, lavender oil, rose oil, bergamot fruit oil, tocopheryl acetate, jasmine oil, arborvitae leaf, and Polygonum multiflorum. This essential oil is rich in effective ingredients that nourish the scalp and repair hair roots, and can be effectively absorbed by the scalp, improving hair health and moisture.

[0006] Although there are many hair / scalp care products on the market, research on the synergistic mechanisms of the ingredients in most products is insufficient. No synergistic combination or reasonable ratio of ingredients has been found that simultaneously soothes the scalp, tightens the scalp, promotes hair follicle vitality, hair growth, and anti-aging, thus failing to achieve multi-target and multi-pathway synergistic effects. Therefore, there is a need to research a massage cream composition with a mild and safe formula, good ingredient synergy, reasonable ingredient ratios, wide application scenarios, and the ability to synergistically soothe and tighten the scalp while promoting hair growth and conditioning, while also providing a pleasant user experience. Summary of the Invention

[0007] This invention addresses the problems existing in the prior art by providing a massage cream composition, preparation method, application, and product that repairs hair, soothes the scalp, and has anti-aging effects. The components in the massage cream composition of this invention work synergistically to achieve the effects of soothing the scalp, improving hair follicle vitality, promoting hair growth, repairing hair strands, repairing the scalp, maintaining the scalp barrier, moisturizing, and firming the scalp while reducing wrinkles and aging.

[0008] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0009] First, the present invention provides a massage cream composition for repairing hair, soothing the scalp, and anti-aging, comprising, by weight: 3-8 parts of Platycladus orientalis leaf extract, 0.5-1.5 parts of peppermint leaf extract, 0.5-1.5 parts of rosemary leaf oil, 0.5-1.5 parts of lavender oil, 0.5-1.5 parts of basil oil, 0.5-1.5 parts of bergamot fruit oil, 0.5-1.5 parts of eucalyptus leaf oil, 0.5-1.5 parts of agarwood extract, 0.5-1.5 parts of ginger root oil, 1.2-3 parts of bisabolol, and 0.5-1.5 parts of tetrahydromethylpyrimidine carboxylic acid.

[0010] Preferably, the massage cream composition, by weight, comprises the following components: 4-6 parts of Platycladus orientalis leaf extract, 0.8-1.2 parts of peppermint leaf extract, 0.8-1.2 parts of rosemary leaf oil, 0.8-1.2 parts of lavender oil, 0.8-1.2 parts of basil oil, 0.8-1.2 parts of bergamot fruit oil, 0.8-1.2 parts of eucalyptus leaf oil, 0.8-1.2 parts of agarwood extract, 0.8-1.2 parts of ginger root oil, 1.5-2.5 parts of bisabolol, and 0.8-1.2 parts of tetrahydromethylpyrimidine carboxylic acid.

[0011] More preferably, the massage cream composition, by weight, comprises the following components: 5 parts of arborvitae leaf extract, 1 part of peppermint leaf extract, 1 part of rosemary leaf oil, 1 part of lavender oil, 1 part of basil oil, 1 part of bergamot fruit oil, 1 part of eucalyptus leaf oil, 1 part of agarwood extract, 1 part of ginger root oil, 2 parts of bisabolol, and 1 part of tetrahydromethylpyrimidine carboxylic acid.

[0012] Furthermore, the present invention provides a method for preparing the above-mentioned massage cream composition, comprising the steps of: mixing arborvitae leaf extract, peppermint leaf extract, rosemary leaf oil, lavender oil, basil oil, bergamot fruit oil, eucalyptus leaf oil, agarwood extract, ginger root oil, bisabolol and tetrahydromethylpyrimidine carboxylic acid to obtain the massage cream composition.

[0013] The present invention then provides the use of the above-described massage cream composition in the preparation of scalp / hair shampoo and conditioner cosmetics.

[0014] Preferably, the scalp / hair shampoo and conditioner in the application is a scalp / hair shampoo and conditioner with the functions of cleansing, oil control, dandruff removal, soothing, itch relief, moisturizing, scalp repair, hair repair, scalp tightening, anti-wrinkle and anti-aging, and maintaining the scalp barrier.

[0015] Preferably, the scalp / hair care cosmetics used in the application are not limited to scalp massage creams, scalp massage lotions, shampoos, scalp cleansing creams, conditioners, hair masks, hair oils, and scalp care serums.

[0016] Finally, the present invention provides a scalp / hair care cosmetic, comprising the above-mentioned massage cream composition and excipients suitable for use in the cosmetic field.

[0017] Preferably, the scalp / hair care cosmetics are not limited to scalp massage creams, scalp massage lotions, shampoos, scalp cleansing creams, conditioners, hair masks, hair oils, and scalp care serums.

[0018] Preferably, the massage cream composition is added at a mass level of 1-3% in the scalp / hair shampoo and conditioner.

[0019] More preferably, in the scalp / hair shampoo and conditioner, the massage cream composition is added at a mass level of 1.6%.

[0020] Preferably, the excipients applicable in the cosmetic field are not limited to solvents, emulsifiers, hair conditioning agents, cooling agents, thickeners, anti-dandruff agents, fragrances, preservatives, pH adjusters, and colorants.

[0021] Compared with the prior art, the present invention has the following beneficial effects: In the massage cream composition of the present invention, 11 ingredients work synergistically under specific formulation conditions. Based on peppermint leaf extract, rosemary leaf oil, lavender oil, and bergamot fruit oil, these ingredients are combined with arborvitae leaf extract, basil oil, eucalyptus leaf oil, agarwood extract, ginger root oil, bisabolol, and tetrahydromethylpyrimidine carboxylic acid. The combination of these ingredients, using specific dosage ratios, brings about synergistic effects in soothing the scalp, reducing inflammation, tightening the scalp, anti-aging, moisturizing and repairing, increasing elastase content, promoting hair growth, repairing hair, and significantly increasing human dermal papilla cells, thus maintaining the scalp barrier. Detailed Implementation

[0022] The following non-limiting embodiments are intended to enable those skilled in the art to gain a more comprehensive understanding of the present invention, but do not limit the invention in any way. The following content is merely an exemplary description of the scope of protection claimed by the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, and such changes should also fall within the scope of protection claimed by the present invention.

[0023] When numerical ranges are given in the embodiments, it should be understood that, unless otherwise stated in the invention, both endpoints of each numerical range and any value between the two endpoints may be selected. Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0024] The present invention will be further described below by way of specific embodiments. Unless otherwise specified, all chemical reagents used in the embodiments of the present invention are obtained through conventional commercial means.

[0025] In the following specific implementation methods, information on the main raw materials is shown in Table 1. Products from different manufacturers do not have a significant impact on the efficacy.

[0026] Table 1

[0027] Example 1 A massage cream composition for repairing hair, soothing the scalp, and anti-aging, comprising, by weight, the following components: 5 parts arborvitae leaf extract, 1 part peppermint leaf extract, 1 part rosemary leaf oil, 1 part lavender oil, 1 part basil oil, 1 part bergamot fruit oil, 1 part eucalyptus leaf oil, 1 part agarwood extract, 1 part ginger root oil, 2 parts bisabolol, and 1 part tetrahydromethylpyrimidine carboxylic acid.

[0028] The above components are mixed evenly to obtain a massage cream composition.

[0029] Example 2 A massage cream composition for repairing hair, soothing the scalp, and anti-aging, comprising, by weight, the following components: 4 parts of Platycladus orientalis leaf extract, 1.2 parts of peppermint leaf extract, 1.2 parts of rosemary leaf oil, 0.8 parts of lavender oil, 0.8 parts of basil oil, 0.8 parts of bergamot fruit oil, 1.2 parts of eucalyptus leaf oil, 1.2 parts of agarwood extract, 0.8 parts of ginger root oil, 1.5 parts of bisabolol, and 1.2 parts of tetrahydromethylpyrimidine carboxylic acid.

[0030] The above components are mixed evenly to obtain a massage cream composition.

[0031] Example 3 A massage cream composition for repairing hair, soothing the scalp, and anti-aging, comprising, by weight, the following components: 6 parts of Platycladus orientalis leaf extract, 0.8 parts of peppermint leaf extract, 0.8 parts of rosemary leaf oil, 1.2 parts of lavender oil, 1.2 parts of basil oil, 1.2 parts of bergamot fruit oil, 0.8 parts of eucalyptus leaf oil, 0.8 parts of agarwood extract, 1.2 parts of ginger root oil, 2.5 parts of bisabolol, and 0.8 parts of tetrahydromethylpyrimidine carboxylic acid.

[0032] The above components are mixed evenly to obtain a massage cream composition.

[0033] Example 4 A massage cream composition for repairing hair, soothing the scalp, and anti-aging, comprising, by weight, the following components: 3 parts arborvitae leaf extract, 1.5 parts peppermint leaf extract, 1.5 parts rosemary leaf oil, 0.5 parts lavender oil, 0.5 parts basil oil, 0.5 parts bergamot fruit oil, 1.5 parts eucalyptus leaf oil, 1.5 parts agarwood extract, 0.5 parts ginger root oil, 1.2 parts bisabolol, and 1.5 parts tetrahydromethylpyrimidine carboxylic acid.

[0034] The above components are mixed evenly to obtain a massage cream composition.

[0035] Example 5 A massage cream composition for repairing hair, soothing the scalp, and anti-aging, comprising, by weight, the following components: 8 parts of Platycladus orientalis leaf extract, 0.5 parts of peppermint leaf extract, 0.5 parts of rosemary leaf oil, 1.5 parts of lavender oil, 1.5 parts of basil oil, 1.5 parts of bergamot fruit oil, 0.5 parts of eucalyptus leaf oil, 0.5 parts of agarwood extract, 1.5 parts of ginger root oil, 3 parts of bisabolol, and 0.5 parts of tetrahydromethylpyrimidine carboxylic acid.

[0036] The above components are mixed evenly to obtain a massage cream composition.

[0037] Comparative Example 1 Unlike Example 1, basil oil, eucalyptus leaf oil, bisabolol and tetrahydromethylpyrimidine carboxylic acid were omitted, while the rest were the same as in Example 1.

[0038] A massage cream composition for repairing hair, soothing the scalp, and anti-aging, comprising, by weight, the following components: 5 parts arborvitae leaf extract, 1 part peppermint leaf extract, 1 part rosemary leaf oil, 1 part lavender oil, 1 part bergamot fruit oil, 1 part agarwood extract, and 1 part ginger root oil.

[0039] The above components are mixed evenly to obtain a massage cream composition.

[0040] Comparative Example 2 Unlike Example 1, basil oil and eucalyptus leaf oil were omitted, while the rest were the same as in Example 1.

[0041] A massage cream composition for repairing hair, soothing the scalp, and anti-aging, comprising, by weight, the following components: 5 parts arborvitae leaf extract, 1 part peppermint leaf extract, 1 part rosemary leaf oil, 1 part lavender oil, 1 part bergamot fruit oil, 1 part agarwood extract, 1 part ginger root oil, 2 parts bisabolol, and 1 part tetrahydromethylpyrimidine carboxylic acid.

[0042] The above components are mixed evenly to obtain a massage cream composition.

[0043] Comparative Example 3 Unlike Example 1, bisabolol and tetrahydromethylpyrimidine carboxylic acid were omitted, while the rest were the same as in Example 1.

[0044] A massage cream composition for repairing hair, soothing the scalp, and anti-aging, comprising, by weight, the following components: 5 parts arborvitae leaf extract, 1 part peppermint leaf extract, 1 part rosemary leaf oil, 1 part lavender oil, 1 part basil oil, 1 part bergamot fruit oil, 1 part eucalyptus leaf oil, 1 part agarwood extract, and 1 part ginger root oil.

[0045] The above components are mixed evenly to obtain a massage cream composition.

[0046] Comparative Example 4 Unlike Example 1, bisabolol was replaced with panthenol.

[0047] A massage cream composition for repairing hair, soothing the scalp, and anti-aging, comprising, by weight, the following components: 5 parts arborvitae leaf extract, 1 part peppermint leaf extract, 1 part rosemary leaf oil, 1 part lavender oil, 1 part basil oil, 1 part bergamot fruit oil, 1 part eucalyptus leaf oil, 1 part agarwood extract, 1 part ginger root oil, 2 parts panthenol, and 1 part tetrahydromethylpyrimidine carboxylic acid.

[0048] The above components are mixed evenly to obtain a massage cream composition.

[0049] Comparative Example 5 Unlike Example 1, Polygonum multiflorum powder was added as a component.

[0050] A massage cream composition for repairing hair, soothing the scalp, and anti-aging, comprising, by weight, the following components: 5 parts Polygonum multiflorum powder, 5 parts Platycladus orientalis leaf extract, 1 part peppermint leaf extract, 1 part rosemary leaf oil, 1 part lavender oil, 1 part basil oil, 1 part bergamot fruit oil, 1 part eucalyptus leaf oil, 1 part agarwood extract, 1 part ginger root oil, 2 parts bisabolol, and 1 part tetrahydromethylpyrimidine carboxylic acid.

[0051] The above components are mixed evenly to obtain a massage cream composition.

[0052] Comparative Example 6 Unlike Example 1, the components of the massage cream composition have different weight parts.

[0053] A massage cream composition for repairing hair, soothing the scalp, and anti-aging, comprising, by weight, the following components: 5 parts arborvitae leaf extract, 1 part peppermint leaf extract, 2 parts rosemary leaf oil, 1 part lavender oil, 1 part basil oil, 4 parts bergamot fruit oil, 1 part eucalyptus leaf oil, 1 part agarwood extract, 3 parts ginger root oil, 0.5 parts bisabolol, and 2.5 parts tetrahydromethylpyrimidine carboxylic acid.

[0054] The above components are mixed evenly to obtain a massage cream composition.

[0055] Efficacy Test 1: Anti-inflammatory and Soothing Effect Test Immortalized keratinocyte line HaCaT cells were cultured in a cell culture incubator at 37°C in 5% CO2 (DMEM medium). The UVB irradiation intensity was 7.15 × 10⁻⁶. -5 J / cm 2 The irradiation source was placed 15 cm away from the cells. During irradiation, the culture medium for each group of cultured cells was aspirated, and the cells were rinsed twice with PBS. PBS was then added again to cover the bottom of the cells to prevent drying. The culture plates were irradiated in a room temperature water bath to avoid overheating after irradiation. After irradiation, the PBS was discarded, and DMEM medium (UVB irradiation group) or medium containing the respective massage cream compositions was added and the cells were cultured for another 24 hours.

[0056] In the study investigating the effect of massage cream compositions on HaCaT cell viability, the experiment was divided into 11 groups: a normal control group (no UVB irradiation), a UVB irradiation group (UVB irradiation + deionized water), Examples 1-5 (UVB irradiation + corresponding massage cream composition 10 μg / mL × 2 mL), and Comparative Examples 1-6 (UVB irradiation + corresponding massage cream composition 10 μg / mL × 2 mL). Each treatment condition had 3 replicates, and the experiment was repeated 3 times. The normal control group did not receive UVB irradiation, while the UVB irradiation group, Examples 1-5, and Comparative Examples 1-6 received UVB irradiation.

[0057] Cell culture medium was collected, and the secretion levels of TNF-α, IL-6, and IL-1β were measured according to the ELISA kit instructions. The results of the secretion levels of TNF-α, IL-6, and IL-1β relative to the normal control group (relative secretion levels) are shown in Table 2. The results for the normal control group were considered as "1.00".

[0058] Table 2

[0059] In Table 2, # and ## This indicates that the data from each group are significantly different from those from the UVB irradiation group. # This indicates that P < 0.05. ## This indicates that P < 0.01; △ and △△ This indicates that the data from each comparative example group showed significant differences compared to the data from Example 1 group. △ This indicates that P < 0.05. △△ This indicates that P < 0.01.

[0060] As shown in Table 2, UVB irradiation significantly increased the secretion of TNF-α, IL-6, and IL-1β in HaCaT cells, indicating that UVB irradiation induced a severe inflammatory response in HaCaT cells. In contrast, the massage cream compositions of Examples 1-5 inhibited UVB irradiation-induced secretion of TNF-α, IL-6, and IL-1β in HaCaT cells, with relatively lower secretion levels than the UVB irradiation group. The massage cream composition of this invention can alleviate the damage to the skin and scalp caused by UVB irradiation-induced oxidative stress and inflammatory damage to HaCaT cells. Furthermore, data from Comparative Examples 1-3 show that even when basil oil and eucalyptus leaf oil, bisabolol and tetrahydromethylpyrimidine carboxylic acid were used separately, the combined effect of reducing TNF-α, IL-6, and IL-1β secretion was still weaker than that of this application, indicating that the components of the massage cream composition of this invention work synergistically to provide a superior anti-inflammatory and soothing effect than when used individually. As can be seen from the technical effects of Comparative Examples 4-6, the components in the massage cream composition were not arbitrarily replaced or added, and the dosage ratio between the components was not conventionally adjusted. The massage cream composition with altered components and dosage ratios had reduced anti-inflammatory and soothing effects, and the secretion of TNF-α, IL-6, and IL-1β was higher than that of the massage cream composition of the examples.

[0061] Effect Test 2: Test on the effect of repairing scalp hair follicles and promoting hair growth. Dermal papilla cells (HDPs) are cells differentiated from dermal connective tissue cells, possessing specific structures and functions, and located at the base of the hair follicle. HDPs activate the hair matrix cells surrounding the hair follicle to maintain and regulate the hair follicle growth cycle. Studies have shown that the number of HDPs in hair follicles increases during the anagen phase. An important function of the dermal papilla is to induce hair follicle regeneration.

[0062] This study evaluated the effects of massage cream compositions on hair growth and hair repair by detecting the influence of samples on the vitality of dermal papilla cells.

[0063] Assay Method: Human dermal papilla cells (HDPs) were added at a rate of 8000 cells per well to 96-well plates and cultured at 37°C in an incubator containing 5% carbon dioxide for 24 hours. The prepared massage cream composition was dissolved in deionized water (1.6% by mass), and 2 mL was added to 10% FBS DMEM culture medium. A blank control group was used, containing no massage cream composition. After 48 hours of culture, cells were stained using the MTT assay, and absorbance at 550 nm was measured using a microplate reader. The cell viability of the blank control group was taken as 100.0%, and the effect of the massage cream composition on HDP cell viability was evaluated.

[0064] The results of HDP cell viability are shown in Table 3.

[0065] Table 3

[0066] In Table 3, # and ## This indicates that the data in each group are significantly different from those in Example 1. # This indicates that P < 0.05. ## This indicates that P < 0.01.

[0067] As can be seen from Table 3, the massage cream composition of the present invention with specific components and proportions has a significant promoting effect on increasing the activity of human dermal papilla cells (HDPs); compared with the comparative example, its HDP activity is significantly improved; in addition, the massage cream composition of Comparative Example 3, after omitting bisabolol and tetrahydromethylpyrimidine carboxylic acid, has similar HDP activity to the massage cream composition of Comparative Example 1 at the same dosage, and the massage cream composition of Comparative Example 2 has a limited increase in HDP activity, while the massage cream composition of the present invention can significantly improve HDP activity. This indicates that the reasonable combination of the components in the massage cream composition of the present invention can achieve multiple effects such as promoting hair follicle activity, repairing hair growth, and soothing and tightening the scalp. The simultaneous and significant improvement of the above-mentioned technical effects is not expected.

[0068] Effect Test 3: Elastase Content Detection Test This experiment used porcine pancreatic elastase as the test subject and N-succinyl-alanine-alanine-p-nitroaniline (AAAPVN, commercially available) as the substrate. Porcine pancreatic elastase can hydrolyze AAAPVN, and its hydrolysis products can cause an increase in absorbance at a wavelength of 420 nm. The absorbance was measured using an enzyme-linked immunosorbent assay (ELISA) reader to evaluate the firming, anti-wrinkle, and anti-aging effects of the massage cream compositions in the examples and comparative examples.

[0069] 1. Solution preparation (1) Prepare Tris-HCl buffer (0.1M pH=7.5): Weigh 2.42g Tris into a beaker, add 200mL of ultrapure water, dissolve completely, and then adjust the pH to 7.5 with concentrated HCl.

[0070] (2) Test samples: Take the massage cream composition separately, dissolve the composition in Tris-HCl buffer, prepare a composition solution with a concentration of 2wt%, and set it as the test sample.

[0071] (3) Prepare substrate solution AAAPVN (2mM): Weigh 4.51mg AAAPVN and dissolve it in 5mL Tris-HCl buffer.

[0072] (4) Prepare porcine pancreatic elastase solution (0.171 U / mL): Dissolve 280 μL of porcine pancreatic elastase stock solution in 10 mL of Tris-HCl buffer.

[0073] 2. Grouping and Sampling The experiment was divided into three groups: the test sample group, the blank control group, and the model control group. Each group had three replicates at the same concentration. The amounts of each solution added are shown in Table 4.

[0074] Table 4

[0075] 3. Measurement Method The reaction was allowed to proceed at room temperature for 15 minutes, and the absorbance was measured at 420 nm using an ELISA reader.

[0076] 4. Calculation formula Elastase inhibition rate (%) = 1 - (A1 - A0) / (A2 - A0); Where: A0 - the average absorbance of the blank control group wells; A1 - The average absorbance of the test sample group; The average absorbance of the A2-model control group holes.

[0077] 5. Experimental Results The results of elastase inhibition rates for each group are shown in Table 5.

[0078] Table 5

[0079] In Table 5, ## This indicates that the data in each group are significantly different from those in the model control group. ## This indicates that P < 0.01; △△ This indicates that the data from each comparative example group showed significant differences compared to the data from Example 1 group. △△ This indicates that P < 0.01.

[0080] As shown in Table 5, the massage cream composition of the present invention has an inhibitory effect on porcine pancreatic elastase, indicating that when the massage cream composition is applied to the skin, it has a significant firming, anti-wrinkle, and anti-aging effect on the scalp. Furthermore, the massage cream composition of the present invention exhibits a superior inhibitory effect on porcine pancreatic elastase compared to the comparative examples. The inhibition rate of the massage cream composition of the present invention against porcine pancreatic elastase is better than the sum of the effects of using the components of Comparative Examples 1-3 separately. The components in the massage cream composition of the present invention have a synergistic effect on firming, anti-wrinkle, and anti-aging, with 1+1>2. As can be seen from the technical effects of Comparative Examples 4-6, the components in the massage cream composition are not arbitrarily replaced or added, and the dosage ratio between components is not conventionally adjusted. The massage cream compositions of the comparative examples, with altered components and dosage ratios, show a reduced inhibitory effect on porcine pancreatic elastase, and the inhibition rate of porcine pancreatic elastase is lower than that of the massage cream compositions of the examples.

[0081] Effect Test 4: Human Use Experiment Fifty-five healthy male and female volunteers aged 20-35 with similar hair quality and experiencing significant hair loss or thinning, who had not undergone hair dyeing, perming, or other hair treatments in the past month, were selected for the test. The volunteers were randomly divided into 11 groups of 5 people each (3 men and 2 women). Each group used a massage cream composition prepared according to Examples 1-5 and Comparative Examples 1-6 (diluted with water to a mass concentration of 1.6% before use, 3g per application) for scalp massage. Massage and shampooing were performed every two days, with each massage lasting 3 minutes. The massage cream composition was applied after shampooing, using the same shampoo. All 11 groups followed the same method and dosage for 30 days. 120 minutes after the last massage and shampooing session, the transdermal water loss (TEWL) value of the scalp was measured, and the loss rate was calculated and averaged. The results of the transdermal water loss (TEWL) data are shown in Table 6 below.

[0082] Test Principle: The German CK multifunctional skin analyzer, model CUTOMETER DUAL MPA580, uses a TEWL (Teint Isobaric Water Loss) probe. The measurement principle of this instrument is based on Fick's diffusion law. TEWL value is an important indicator of the skin barrier's condition; the more intact the skin's protective layer, the higher the moisture content and the lower the TEWL value. It characterizes the skin barrier repair function of the test sample; the lower the value, the less moisture loss and the stronger the scalp's repair ability; conversely, the higher the value, the weaker the scalp's repair ability.

[0083] Table 6

[0084] In Table 6, # and ## This indicates that the data in each group are significantly different from those in Example 1. # This indicates that P < 0.05. ## This indicates that P < 0.01.

[0085] As shown in Table 6, the massage cream composition of the present invention exhibits significantly lower water loss compared to the comparative group. The comparative group, with altered composition, increased ingredients, or changed weight ratios, shows significantly increased water loss, indicating a weaker scalp repair ability. Conversely, the massage cream composition of the present invention demonstrates a significantly reduced transdermal water loss rate, indicating a stronger scalp repair ability. The massage cream composition of the present invention has a reasonable formulation and synergistic effect in repairing the scalp barrier and improving moisturizing performance.

[0086] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A massage cream composition for repairing hair, soothing the scalp, and anti-aging, characterized in that, By weight, the ingredients include: 3-8 parts of Platycladus orientalis leaf extract, 0.5-1.5 parts of peppermint leaf extract, 0.5-1.5 parts of rosemary leaf oil, 0.5-1.5 parts of lavender oil, 0.5-1.5 parts of basil oil, 0.5-1.5 parts of bergamot fruit oil, 0.5-1.5 parts of eucalyptus leaf oil, 0.5-1.5 parts of agarwood extract, 0.5-1.5 parts of ginger root oil, 1.2-3 parts of bisabolol, and 0.5-1.5 parts of tetrahydromethylpyrimidine carboxylic acid.

2. The massage cream composition according to claim 1, characterized in that, By weight, the composition includes: 4-6 parts of Platycladus orientalis leaf extract, 0.8-1.2 parts of peppermint leaf extract, 0.8-1.2 parts of rosemary leaf oil, 0.8-1.2 parts of lavender oil, 0.8-1.2 parts of basil oil, 0.8-1.2 parts of bergamot fruit oil, 0.8-1.2 parts of eucalyptus leaf oil, 0.8-1.2 parts of agarwood extract, 0.8-1.2 parts of ginger root oil, 1.5-2.5 parts of bisabolol, and 0.8-1.2 parts of tetrahydromethylpyrimidine carboxylic acid.

3. The massage cream composition according to claim 2, characterized in that, The product comprises, by weight, 5 parts of Platycladus orientalis leaf extract, 1 part of peppermint leaf extract, 1 part of rosemary leaf oil, 1 part of lavender oil, 1 part of basil oil, 1 part of bergamot fruit oil, 1 part of eucalyptus leaf oil, 1 part of agarwood extract, 1 part of ginger root oil, 2 parts of bisabolol and 1 part of tetrahydromethylpyrimidine carboxylic acid.

4. A method for preparing the massage cream composition according to any one of claims 1-3, characterized in that, The steps include: mixing arborvitae leaf extract, peppermint leaf extract, rosemary leaf oil, lavender oil, basil oil, bergamot fruit oil, eucalyptus leaf oil, agarwood extract, ginger root oil, bisabolol, and tetrahydromethylpyrimidine carboxylic acid to obtain a massage cream composition.

5. The use of the massage cream composition according to any one of claims 1-3 in the preparation of scalp / hair shampoo and conditioner cosmetics.

6. The application according to claim 5, characterized in that, The scalp / hair care products mentioned above are scalp / hair care products with functions of cleansing, oil control, dandruff removal, soothing, itch relief, moisturizing, scalp repair, hair repair, scalp tightening, anti-wrinkle and anti-aging, and maintaining the scalp barrier.

7. The application according to claim 5, characterized in that, The scalp / hair care cosmetics mentioned are not limited to scalp massage creams, scalp massage gels, shampoos, scalp cleansing creams, conditioners, hair masks, hair oils, and scalp care serums.

8. A scalp / hair shampoo and conditioner, characterized in that, The components include the massage cream composition according to any one of claims 1-3 and excipients suitable for use in the cosmetic field.

9. The scalp / hair care cosmetic according to claim 8, characterized in that, This includes, but is not limited to, scalp massage creams, scalp massage gels, shampoos, scalp cleansing creams, conditioners, hair masks, hair oils, and scalp care serums.

10. The scalp / hair care cosmetic according to claim 8, characterized in that, The massage cream composition is added at a rate of 1-3% by mass.

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