New application of electrolyzed water

Abstract

The invention discloses a novel application of electrolyzed water, the electrolyzed water is electrolyzed water spray or spray water instantly manufactured by using an electrolyzed water device, has a high oxidation potential of more than or equal to 1200mv, and does not release detectable gas ozone of more than or equal to 0.1 mg / m < 3 > to the surrounding environment; the electrolyzed water spray or spray water also has detectable non-volatile oxidation capacity which is equivalent to 2.18 ppm to 4.22 ppm of dissolved ozone; the high oxidation potential greater than or equal to 1200mv reflects the total oxidation capacity of all substances in the aqueous solution; the high oxidation potential greater than or equal to 1200 mv and the oxidation capacity are rapidly attenuated along with time extension and temperature rise; an electrolysis raw material of the water electrolysis device is normal saline; the anode surface of the water electrolysis device is made of a conductive diamond material layer; the electrolyzed water is used for relieving or treating skin mucosa inflammatory diseases; the skin mucosa inflammatory disease is skin itch or skin redness and swelling. When the electrolyzed water is applied to a human body, the electrolyzed water is safe to a user and harmless to the environment of the user, and meets national declaration and commercialization requirements.

Description

This application is a divisional application. The original application has the application number 2023103743895, the application date is April 10, 2023, and the invention title is "A New Application of Electrolysis of Water". Technical Field

[0001] This invention relates to the fields of water electrolysis and clinical research, and in particular to a new application of water electrolysis. Background Technology

[0002] Gaseous ozone and dissolved ozone have a wide range of applications, including odor removal, water treatment, and the elimination of bacteria, viruses, and fungi from surfaces and the air in daily life and industry. However, their application to humans faces difficulties, generally failing to obtain approval from the Food and Drug Administration and thus hindering commercialization.

[0003] In recent years, researchers have discovered a type of water electrolysis device (https: / / www.deposon.com.cn; https: / / www.deposon.com). This type of device utilizes a wear-resistant, heat-resistant, and conductive diamond (blue diamond) anode coating as the conductive anode material. The electrolyzed water produced is then used for disinfecting hard object surfaces. However, current applications of this device are limited to using the ozone-containing electrolyzed water it produces for hard object surface disinfection.

[0004] In addition, inflammatory diseases of the skin and mucous membranes are generally treated with drugs, but drug treatment is prone to drug resistance. Therefore, there is an urgent need to develop some new therapeutic substances. Summary of the Invention

[0005] The technical problem to be solved by this invention is to provide a new application of electrolyzed water with special properties produced by an electrolysis device, which is safe for users, harmless to the user's environment, and meets national application and commercialization requirements.

[0006] During the research, this invention unexpectedly discovered that a water electrolysis device made of a conductive diamond anode exhibits a high oxidation potential of ≥1200mV in the instantaneously generated electrolyzed water spray or jet, and unexpectedly, it does not release detectable levels of ≥0.1mg / m³ into the surrounding environment. 3 The gas ozone.

[0007] This research is completely different from previous understanding of the electrolyzed water produced by this type of device. It breaks through the previous research that believed that this type of water electrolysis device was used to produce ozone water. Due to the safety issues of ozone water, those skilled in the art stopped applying this type of device to the human body.

[0008] Based on the above new findings, this invention provides a new application for this type of water electrolysis.

[0009] A novel application of water electrolysis, wherein the electrolyzed water is an electrolyzed water spray or mist produced instantaneously using a water electrolysis device, possessing a high oxidation potential of ≥1200mV, but without releasing detectable ozone gas at ≥0.1mg / m3 into the surrounding environment; the high oxidation potential of ≥1200mV reflects the total oxidizing capacity exhibited by all substances in the aqueous solution; the high oxidation potential of ≥1200mV decays rapidly with time and temperature increases; the anode surface of the water electrolysis device is made using a conductive diamond material layer; the electrolyzed water is used to alleviate or treat inflammatory diseases of the skin and mucous membranes; or the electrolyzed water is used to prepare drugs for alleviating or treating inflammatory diseases of the skin and mucous membranes.

[0010] The electrolyzed water spray or water jet contains a detectable non-volatile oxidizing capacity equivalent to 2.18 ppm to 4.22 ppm of dissolved ozone; the oxidizing capacity decreases rapidly with time and temperature.

[0011] The electrolytic raw material of the water electrolysis device is physiological saline; the electrolyzed water spray or spray contains detectable non-volatile oxidizing power equivalent to 3.62ppm-4.22ppm of dissolved ozone.

[0012] The detectable non-volatile oxidizing capacity, equivalent to 2.18 ppm to 4.22 ppm of dissolved ozone, was detected using an iodometric dissolved ozone meter.

[0013] The electrolyzed water spray or mist has a pH of 7.6-8.6, which is between neutral and slightly alkaline, and also releases negative air ions.

[0014] The water electrolysis device is a low-pressure water electrolysis device.

[0015] The inflammatory diseases of the skin and mucous membranes are characterized by itchy skin or red and swollen skin.

[0016] The new application is specifically as follows:

[0017] (1) Relieve or treat itching or chronic skin and mucous membrane inflammation in elderly patients with groin eczema, neurodermatitis or psoriasis;

[0018] (2) Relieves itching on the soles of the feet or promotes the healing of skin wounds caused by athlete's foot;

[0019] (3) Relieves gum discomfort, redness, swelling, and bleeding in patients with periodontitis;

[0020] (4) Reduce redness and swelling of the skin around the wound caused by laser skin surgery;

[0021] (5) Reduces perineal itching;

[0022] (6) Reduce scalp discomfort or itching;

[0023] (7) Reduce the number of E. coli or the titer of human influenza virus H1N1 on human hands;

[0024] (8) Relieves itching or discomfort in the nasopharynx;

[0025] Or (9) reduce inflammatory erythema in the diaper area.

[0026] The wear-resistant, heat-resistant, and conductive diamond material coating of the water electrolysis device in this invention, serving as the anolyte conductive diamond material, produces an electrolyzed water spray or water spray that has a high oxidation potential of ≥1200mV. Simultaneously, using an iodometric dissolved ozone meter, it can detect a non-volatile oxidizing capacity equivalent to 2.18ppm-4.22ppm of dissolved ozone, without releasing detectable levels of ≥0.1mg / m³ into the surrounding environment. 3 The device contains ozone (O3). It is safe due to its low ozone content and poses no harm to the user's environment, allowing for national approval and commercialization. It has a synergistic effect on relieving and treating inflammatory skin and mucous membrane diseases, such as itchy or swollen skin, including intractable skin inflammation and itching, oral inflammation, rhinitis, fungal athlete's foot, and scalp diseases, as well as bacterial and viral infections. This greatly expands the application of the device and provides a new, safe, and effective treatment for these various diseases. Attached Figure Description

[0027] The above is merely an overview of the technical solution of the present invention. In order to better understand the technical means of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0028] Figure 1 This is a graph showing the viral titer (TCID50) of human influenza virus H1N1 in water samples collected in Example 10. Detailed Implementation

[0029] The instruments and equipment used in the embodiments of the present invention are as follows:

[0030] I. Huankai Ozone Colorimeter (Pocket Ozone Colorimeter, Iodometric Dissolved Ozone Analyzer) (Guangdong Huankai Biotechnology Co., Ltd. 0-2.5mg / L IIS-301): This pocket ozone colorimeter is a handheld LED light source colorimeter that accurately detects ozone in water samples, with a measurement range of 0.01~2.50mg / L.

Test Principle

Measurement Range

Standard

Application Scope

Product Features

[0031] II. Portable Oxidation-Reduction Potential Meter (Liquid MV Value Detector) (Shenzhen Kodida Electronics Co., Ltd. CT-8022 Pen-type ORP Meter):

Test Principle

Measurement Range

[0032] III. Ozone Gas Detector (Jinan Keyu Electronic Information Technology Co., Ltd., Prisen PR-MG41-O3):

Testing Principle

Measurement Range

[0033] IV. Dissolved Oxygen Meter (Hangzhou Qiwei Instruments Co., Ltd., Xima AR8010):

Testing Principle

Measurement Range

[0034] V. Hydrogen-Rich Detector (YY400, Henan Rongmeida Environmental Protection Technology Co., Ltd.):

Testing Principle

Measurement Range

[0035] VI. Hydrogen Peroxide Solution Concentration Meter (Dezhou Zhongyuan Electronics Co., Ltd. DR803):

Test Principle

Test Range

[0036] VII. Negative Ion Detector (Beijing Dongchuang Xuxin Measurement and Control Technology Co., Ltd. IMHO01):

Testing Principle

Testing Range

[0037] 8. Negative ion water sprayer (made in Japan).

[0038] 9. In the embodiments of the present invention, tap water and a novel water electrolysis device are used; the novel water electrolysis device (CN215308550U; https: / / www.deposon.com.cn; https: / / www.deposon.com) is a small handheld device that uses a diamond anode plate and uses a low-pressure electrolysis method to produce electrolyzed water spray or water spray.

[0039] Example 1:

[0040] Objective: To identify the nonspecific oxidizing capacity of water spray or water spray.

[0041] method:

[0042] The oxidation capacity and oxidation potential (liquid mV) of the following water samples (instantly produced electrolyzed water, hydrogen peroxide liquid, dissolved ozone water (water bubbled for 10 minutes), dissolved oxygen water (water bubbled for 10 minutes), dissolved hydrogen water (water bubbled for 10 minutes), dissolved hydrogen peroxide water, and instantly produced negative ion water) equivalent to dissolved "ozone" ppm were determined using a pocket ozone colorimeter (iodometric dissolved ozone meter) and a liquid mV value detector. Note: When sampling instant-produced electrolyzed water, the sample was directly sprayed / injected into the sampling bottle using the equipment.

[0043] Results: Table 1-1 shows the results of the determination of the oxidation capacity and oxidation potential (liquid mv) of dissolved ozone in water samples by a pocket ozone colorimeter (iodometric dissolved ozone meter) and an oxidation potential (liquid mv) detector.

[0044]

[0045]

[0046] Note: The instant-made electrolyzed water sprayer dispenses 23 ml of water in 10 seconds, and the instant-made electrolyzed water atomizer dispenses 4 ml of water in 10 seconds. Where n=4 indicates that it is repeated 4 times.

[0047] Table 1-2 shows the results of determination of the oxidizing capacity and oxidation potential (liquid mv) of detectable dissolved "ozone" ppm in water samples at 20℃ and 30℃ using a pocket ozone colorimeter (iodometric dissolved ozone meter) and an oxidation potential (liquid mv) detector.

[0048]

[0049] Note: The instantaneous electrolyzed water sprayer produces 23 ml of water in 10 seconds, while the instantaneous electrolyzed water sprayer produces 4 ml of water in 10 seconds. n = 4 indicates 4 repetitions.

[0050] Discussion: N,N-Diethyl-p-phenylenediamine (DPD) can be oxidized by various oxidizing disinfectants, including ozone, to produce a red color. Because it does not specifically react with ozone, it is not listed as a standard method for ozone detection in water in my country. This study used this non-specific reaction ozone dissolution method to determine the non-volatile oxidizing capacity equivalent to dissolved ozone (ppm) in a newly produced high-oxidation-potential electrolyzed water.

[0051] Hydroxyl radicals are characteristic of advanced oxidation processes, but their short half-life makes them difficult to measure directly. This study indirectly determined the advanced oxidation capacity of hydroxyl radicals using a high oxidation-reduction potential (ORP) ≥1200 mV and oxidizing power.

[0052] Conclusions: 1. The Huankai ozone colorimeter (pocket ozone colorimeter) used in this study is a non-specific oxidizing capacity measuring device for dissolved water and cannot be used for specific determination of dissolved ozone; 2. Electrolyzed water produced instantly using tap water and a new type of electrolyzed water device is a highly electron-deficient electrolyzed water spray or water spray with a high oxidation potential ≥1200mV. It also contains an oxidizing capacity equivalent to 2.18ppm-2.72ppm of dissolved "ozone" (2.18ppm-2.28ppm for spray and 2.62ppm-2.72ppm for water spray). Its high oxidation potential reflects the dissolved ozone content in the water. The total oxidizing capacity exhibited by all substances in the liquid; its high oxidation potential (ORP) and oxidizing capacity rapidly decay with time and temperature increase, exhibiting advanced oxidizing characteristics of hydroxyl radicals and oxygen radicals; 3. Because the oxidizing capacity of this instant-generated electrolyzed water spray or water spray is equivalent to dissolved "ozone" 2.18ppm-2.72ppm (very low content), the inventors believe that the antimicrobial and anti-inflammatory effects of this instant-generated electrolyzed water spray or water spray on skin and mucous membranes are mainly related to the high oxidation potential ≥1200mv, that is, related to the advanced oxidation of hydroxyl radicals generated by it.

[0053] Example 2

[0054] Objective: To release ozone gas from electrolyzed water with an immediate oxidation potential of ≥1200mV and an oxidation capacity equivalent to approximately 2.23ppm of dissolved ozone.

[0055] Methods: This study used the following equipment and methods to determine the ozone gas release from electrolyzed water with a high oxidation potential ≥1200mV and a detectable oxidation capacity equivalent to approximately 2.23ppm of dissolved "ozone": Same as Example 1.

[0056] Ozone gas was detected at a distance of 10 cm outside the nozzle by spraying the following liquids (electrolyzed water, hydrogen peroxide liquid, and negative ion water prepared on-site in this study) for 10 seconds using an ozone gas detector, a negative ion detector, and spraying for 10 seconds.

[0057] Using an ozone gas detector, a negative ion detector, and the following gases (pure oxygen, ozone, and hydrogen), measure "ozone gas" and negative ions within 10 seconds.

[0058] result:

[0059] Table 2-1 shows the results of measurements of gaseous ozone and negative oxygen ions in electrolyzed water, hydrogen peroxide liquid, negative ion water spray, pure oxygen, ozone, and hydrogen gas, which were generated on-site in this study and had a high oxidation potential ≥1200mV and a detectable oxidation capacity equivalent to about 2.23ppm of dissolved "ozone".

[0060]

[0061] Where n = 4 means repeating 4 times.

[0062] Discussion: The results indicate that the electrolyzed water spray produced in this study using the described electrolysis device and tap water, exhibiting a high oxidation potential ≥1200mV and a detectable oxidation capacity equivalent to approximately 2.23ppm of dissolved ozone, did not contain volatile dissolved ozone. Similarly, the effects were the same when using electrolyzed water spray and when using pure water, saline solution, or mineral water as the electrolysis material.

[0063] Conclusion: This study successfully produced an electrolyzed water spray with a high oxidation potential ≥1200mV and a detectable oxidation capacity equivalent to approximately 2.23ppm of dissolved ozone using the described electrolysis device and tap water. This spray did not release detectable levels of ≥0.1mg / m³ of ozone into the surrounding environment. 3 The gaseous ozone; when electrolyzing water and using pure water, saline or mineral water as the electrolysis material, the effect is the same, it is safe for users and has no harm to the user's environment, and meets the national application and commercialization requirements.

[0064] Example 3

[0065] Objective: To determine the composition and define the components of an electrolyzed water spray or water jet.

[0066] background:

[0067] The oxidation-reduction potential (ORP) is used to reflect the macroscopic oxidizing power of all substances in an aqueous solution. A high ORP indicates a high concentration of dissolved oxygen or oxidizing substances; that is, a high ORP reflects the total oxidizing capacity of all substances in the aqueous solution.

[0068] Early acidic electrolyzed water was produced using raw water with added NaCl, resulting in acidic electrolyzed water with both physical (high electron deficiency and high redox potential) and chemical (low concentration of active chlorine and hypochlorous acid) effects. In 2011, my country formulated GB28234, "Safety and Hygiene Standard for Acidic Electrolyzed Water Generators," which specifies the following indicators for acidic electrolyzed water generation: pH 2.0-3.0, available chlorine concentration (acc) 60±10 mg / L, and redox potential ≥1100 mV. Studies have shown that the bactericidal and disinfecting function of acidic electrolyzed water is determined by its unique multi-factor structure: electron deficiency (orp), available chlorine concentration (acc), and acidity (pH). When these three indicators are in appropriate proportions and maintain a dynamic balance, they mutually promote and enhance each other's effectiveness. A single indicator at low concentrations is insufficient for sterilization.

[0069] The novel water electrolysis device (CN215308550U) used in this invention utilizes a wear-resistant, heat-resistant, and conductive diamond (blue diamond) material coating as the anode conductive electrode to generate electrolyzed water for disinfecting hard object surfaces. The inventor of this device identified it as ozone water. However, the test results of this application (Examples 1 and 2) show that the electrolyzed water produced by this device contains almost no dissolved ozone and does not release gaseous ozone, which differs from the test results and conclusions of the inventor of this device. Upon verification with the inventor of this device, it was found that the inventor used a non-specific iodometric method to determine the oxidant concentration, and specifically misjudged the dissolved ozone (Examples 1 and 2). Furthermore, using chlorine-free raw water for electrolysis, the electrolyzed water produced by this device is chlorine-free, unlike acidic electrolyzed water produced by adding NaCl.

[0070] method:

[0071] This study used the following equipment and methods to determine the composition of electrolyzed water spray or water spray: same as in Example 1.

[0072] This study utilizes tap water and the novel water electrolysis device to instantly generate an electrolytic spray or water jet. The key electrode (anode) of the novel water electrolysis device involves the use of wear-resistant and heat-resistant conductive diamond material.

[0073] (CN215308550U; https: / / www.deposon.com.cn; https: / / www.deposon.com).

[0074] The two new types of water electrolysis devices have significantly different output volumes. One is a handheld spray device with an output of 4.2±0.1 ml (g) within 10 seconds, while the other is a handheld water sprayer device with an output of 23±1 ml (g) within 10 seconds.

[0075] result:

[0076] Table 3-1 shows the detectable oxidation capacity and oxidation potential of electrolyzed water produced instantly using tap water in this study, equivalent to dissolved ozone ppm.

[0077]

[0078]

[0079] Table 3-2 shows the detectable oxidation capacity and oxidation potential of electrolyzed water produced instantly using physiological saline in this study, equivalent to dissolved ozone ppm.

[0080]

[0081] Table 3-3 shows the detectable oxidation capacity and oxidation potential of electrolyzed water produced on-site using mineral water in this study, equivalent to dissolved ozone ppm.

[0082]

[0083] Table 3-4 shows the detectable oxidation capacity and oxidation potential of electrolyzed water produced instantly using pure water in this study, equivalent to dissolved ozone ppm.

[0084]

[0085] Table 3-5. Determination of dissolved O2 in electrolyzed water produced on-site using pure water in this study.

[0086]

[0087] Note: The dissolved oxygen in pure water is approximately 9 mg / L.

[0088] The results showed that the oxygen content of electrolyzed water produced using pure water did not increase significantly.

[0089] Table 3-6. Determination of hydrogen peroxide in electrolyzed water produced on-site using pure water in this study.

[0090]

[0091] Table 3-7. Determination of dissolved H2 in electrolyzed water produced on-site using pure water in this study.

[0092]

[0093] In Tables 3-1 to 3-7, n=4 indicates that the number of repetitions is 4.

[0094] discuss:

[0095] The hydroxyl radical is an important reactive oxygen species, which, according to its molecular formula, consists of a hydroxyl group (OH-). - Hydroxyl radicals are formed by losing an electron. They possess extremely strong electronic capabilities, i.e., oxidizing power. The anode of a water electrolysis device can directly or indirectly generate hydroxyl radicals with strong oxidizing activity. Advanced oxidation is primarily characterized by oxidation reactions dominated by hydroxyl radicals. Oxidation reactions caused by hydroxyl and oxygen radicals have short half-lives and decay rapidly with increasing time and temperature.

[0096] Electrolyzed water, instantaneously produced using tap water and a novel electrolysis device, is a highly electron-deficient water spray with a high oxidation potential (ORP) ≥1200mV. After spraying and pumping, it produces a detectable non-volatile oxidizing capacity equivalent to 2.18ppm-2.72ppm dissolved ozone. This ≥1200mV oxidation-reduction potential reflects the total oxidizing capacity exhibited by all substances in the aqueous solution. The high ORP electrolyzed water exhibits a rapid decline in oxidation potential (≥1200mV) and oxidizing capacity with increasing temperature and time, leaving no residue. It possesses advanced oxidizing characteristics of hydroxyl and oxygen free radicals. This high ORP electrolyzed water spray also releases negative air ions, but does not release detectable (>0.1mg / m³) ions into the surrounding environment. 3 The ozone gaseous content meets environmental safety requirements.

[0097] Conclusion: This invention discloses a new application of electrolyzed water, wherein the electrolyzed water is an electrolyzed water spray or spray produced instantly using tap water, saline, mineral water, or pure water and an electrolyzed water device. After being sprayed, the generated high oxidation potential of ≥1200mV contains detectable non-volatile oxidizing capacity equivalent to 2.18ppm-2.72ppm of dissolved ozone, but does not release detectable gaseous ozone into the surrounding environment. This high oxidation potential of ≥1200mV and oxidizing capacity decays rapidly with time and temperature increases.

[0098] The inventors unexpectedly discovered that the electrolyzed water is effective in relieving or treating inflammatory skin and mucous membrane diseases manifested as itching or redness. Furthermore, the inventors expanded the research to include mild senile groin eczema, neurodermatitis and psoriasis, mild dermatitis, mild periodontitis, inflammatory wounds resulting from laser skin surgery, and genital and scalp itching, as well as the elimination of bacteria and viruses on hands or skin, all of which showed significant technical efficacy. This study is an open-label clinical trial registered at www.clinicaltrials.com, comparing the effects before and after treatment in a single-arm clinical trial or an open-label two-arm clinical trial comparing the effects of two groups. Examples 4-12 are provided below for further explanation.

[0099] Example 4:

[0100] Objective: To conduct a clinical study on the reduction of pruritus in patients with mild senile groin eczema, neurodermatitis, and psoriasis using a novel electrolyzed water spray or mist.

[0101] method:

[0102] This study used tap water and a novel water electrolysis device (CN21530855 U) to instantly produce electrolyzed water. This electrolyzed water is highly electron-deficient and has a high oxidation potential (ORP) ≥1200mV. The resulting spray and water contain detectable non-volatile oxidizing power equivalent to 2.18ppm-2.72ppm of dissolved ozone (approximately 2.23ppm for spray and 2.67ppm for water). This high ORP electrolyzed water exhibits a high oxidation potential ≥1200mV and its oxidizing power rapidly decreases with time and temperature, leaving no residue. It possesses advanced oxidizing characteristics of hydroxyl and oxygen free radicals. This high ORP electrolyzed water spray also releases negative air ions, but does not release detectable (>0.1mg / m³) ions into the surrounding environment. 3 The gaseous ozone produced meets environmental safety requirements. The key electrode (anode) portion of the novel water electrolysis device involves the use of wear-resistant, heat-resistant conductive diamond material. In this study, the clinical inclusion criteria for treatment using the novel water electrolysis device and water spray therapy included patients with mild senile groin eczema, neurodermatitis, and psoriasis.

[0103] This is an open-label, single-arm, pre- and post-treatment comparative study (NCT05770271). The treatment included 14 patients with mild senile groin eczema (n=7), neurodermatitis (n=3), and psoriasis (n=4), 6 males and 8 females, with a mean age of 50 ± 15 years. The treatment involved spraying the affected area with a spray until half the device's volume (250 ml) was used, twice daily for 10 days. After treatment, researchers followed up with patients and recorded their self-reported itch level one hour after the first treatment, using an itch scale. This itch scale was modified from the National Pain Rating Scale (NPRS), the most commonly used pain assessment scale in clinical practice, used by physicians to unilaterally assess the intensity of pain in patients. Researchers used numbers 0-10 to represent patients' self-rated itch levels, with "0" indicating no itching and "10" indicating the most intense itching. In addition to assessing the severity of itching, researchers also needed to measure the improvement in patients' chronic skin inflammation on day 10 after 10 days of continuous treatment. This included darkening or redness around the skin, swelling and dryness, as well as the extent of damage and reduction in the affected skin surface. Researchers used a number scale from 0 to 10 to score the improvement in patients' chronic skin inflammation, with higher numbers indicating worse recovery.

[0104] result:

[0105] Table 4-1. Itching scale for patients with senile groin eczema, neurodermatitis and psoriasis

[0106]

[0107] Table 4-2, Scale for the Degree of Improvement in Chronic Skin Inflammation

[0108]

[0109] Discussion: 1. Indirect effects: Microbial endotoxins trigger excessive resistance from the human immune system, causing inflammation and leading to disease; oxidation reactions eliminate microorganisms and endotoxins, reducing inflammation; 2. Direct effects: The antipruritic reaction is rapid, occurring within 30 seconds after spraying; the inventors speculate that reactive oxygen free radicals in the body have certain immune and signal transduction functions or inactivate inflammatory mediators, inhibiting the inflammation that mediates itching.

[0110] Conclusion: This novel electrolyzed water spray can significantly reduce itching in patients with mild senile groin eczema, neurodermatitis and psoriasis, with no observed adverse reactions. Water spray can also achieve similar effects.

[0111] Example 5

[0112] Objective: To alleviate itching on the soles of the feet and promote the healing of skin wounds caused by athlete's foot with a novel electrolyzed water spray or mist.

[0113] method:

[0114] This study used tap water and a novel water electrolysis device (CN215308550U) with an electrolysis voltage of ≥1200mV for both spraying and water spraying. The resulting product, after spraying and pumping, contained detectable levels of non-volatile oxidizing power equivalent to 2.18ppm-2.72ppm dissolved ozone (approximately 2.23ppm for spray and 2.67ppm for water spray). This high oxidation potential (≥1200mV) electrolyzed water exhibited a high oxidation potential and oxidizing power that rapidly decreased with time and temperature increases, leaving no residue. It possessed advanced oxidizing characteristics of hydroxyl and oxygen free radicals. This high oxidation potential (ORP) electrolyzed water spray also released negative air ions, but did not release detectable (>0.1mg / m³) ions into the surrounding environment. 3 The gaseous ozone produced meets environmental safety requirements. The key electrode (anode) portion of the novel water electrolysis device involves the use of wear-resistant, heat-resistant conductive diamond material. In this study, this novel water electrolysis device and water spray were used to treat mild dermatitis, relieve itching on the soles of the feet, and promote the healing of skin wounds caused by athlete's foot.

[0115] This is an open-label, single-arm, pre- and post-treatment comparative study (NCT05770245). The study included 14 patients with athlete's foot and significant itching symptoms (6 males, 8 females, mean age 45 ± 25 years). Treatment involved spraying the affected area with a sprayer until half the device's volume (250 ml) was used, twice daily for 7 days. After treatment, researchers followed up with patients and recorded their self-reported itching level 1 hour after the first treatment, using an itch scale. This itch scale was modified from the National Pain Rating Scale (NPRS), the most commonly used pain assessment scale in clinical practice, used by physicians to unilaterally assess the intensity of pain in patients. Researchers used a number range of 0-10 to represent patients' self-reported itching level, with "0" indicating no itching and "10" indicating the most intense itching. In addition to assessing the severity of itching, researchers also needed to measure the healing of dermatitis patients' affected skin on day 7 after seven consecutive days of treatment. This involved measuring the replacement of lesions with new, growing skin, including surrounding dark or red skin, swelling and dryness, and the reduction in the area of ​​lesions. Researchers used a scale of 0-10 to represent the healing score of skin trauma caused by athlete's foot, with higher numbers indicating worse improvement.

[0116] result:

[0117] Table 5-1, Itching Scale for Patients with Athlete's Foot

[0118]

[0119] Table 5-2. Scale for the degree of improvement of skin trauma caused by athlete's foot

[0120]

[0121] Discussion: It has antifungal properties, therefore it is effective in treating athlete's foot.

[0122] Conclusion: This novel electrolyzed water spray significantly reduced itching caused by athlete's foot and promoted the healing of skin wounds caused by athlete's foot, with no observed adverse reactions. Water spray can also achieve similar effects.

[0123] Example 6

[0124] Objective: To conduct a clinical study on the reduction of gingival discomfort and / or redness and bleeding in patients with mild gingivitis by novel electrolyzed water spray or water spray.

[0125] method:

[0126] This study used tap water and a novel water electrolysis device (CN215308550U) to instantly produce electrolyzed water. This electrolyzed water is highly electron-deficient and has a high oxidation potential (ORP) ≥1200mV. The resulting spray and water contain detectable levels of non-volatile oxidizing power equivalent to 2.18ppm-2.72ppm dissolved ozone (approximately 2.23ppm for spray and 2.67ppm for water). The high ORP (≥1200mV) oxidation potential and oxidizing power of this electrolyzed water rapidly decrease with time and temperature increases, leaving no residue. It exhibits advanced oxidizing characteristics of hydroxyl and oxygen free radicals. This high ORP electrolyzed water spray also releases negative air ions, but does not release detectable (>0.1mg / m³) ions into the surrounding environment. 3 The gaseous ozone produced meets environmental safety requirements. The key electrode (anode) of the novel water electrolysis device involves the use of wear-resistant, heat-resistant conductive diamond material. In this study, this novel water electrolysis device and water spray were used to treat mild gingivitis.

[0127] This is an open-label, single-arm, pre- and post-treatment comparative study (NCT05770258). The study included 14 patients with mild periodontitis and gingival discomfort (7 males, 7 females, mean age 52 ± 16 years). Treatment involved spraying the affected area or oral cavity with a sprayer until 150 ml of water was used up, twice daily for 10 days. After treatment, researchers followed up with patients and recorded the degree of gingival discomfort one hour after the first treatment, using a discomfort scale. This discomfort scale was modified from the National Pain Rating Scale (NPRS), the most commonly used pain assessment scale in clinical practice, used by physicians to unilaterally assess the intensity of pain in patients. Researchers used numbers 0-10 to represent patients' self-ratings of discomfort, with "0" indicating no discomfort and "10" indicating the most severe discomfort. In addition to assessing gingival discomfort, researchers also measured gingival redness and bleeding in patients with mild periodontitis on day 10 after the 10-day treatment period.

[0128] result:

[0129] Table 6-1, Gingival Discomfort Scale

[0130]

[0131] Table 6-2 Symptom Improvement Scale for Patients with Mild Periodontitis

[0132]

[0133] Discussion: 1. Indirect effects: Microbial endotoxins trigger excessive resistance from the human immune system, causing inflammation and leading to disease; oxidation reactions eliminate microorganisms and endotoxins, reducing inflammation; 2. Direct effects: The antipruritic reaction is rapid, occurring within 30 seconds after spraying; the inventors speculate that reactive oxygen free radicals in the body have certain immune and signal transduction functions or inactivate inflammatory mediators, inhibiting the inflammation that mediates itching.

[0134] Conclusion: This novel electrolyzed water spray significantly reduced gingival discomfort and / or redness and bleeding in patients with mild gingivitis, with no observed adverse reactions. Water spray can also achieve similar effects.

[0135] Example 7

[0136] Objective: To conduct a clinical study on the reduction of peri-injury and redness of the skin around the wound caused by laser skin surgery using a novel electrolyzed water spray or water mist.

[0137] method:

[0138] This study used tap water and a novel water electrolysis device (CN215308550U) to instantly produce electrolyzed water. This electrolyzed water is highly electron-deficient and has a high oxidation potential (ORP) ≥1200mV. The resulting spray and water contain detectable levels of non-volatile oxidizing power equivalent to 2.18ppm-2.72ppm dissolved ozone (approximately 2.23ppm for spray and 2.67ppm for water). The high ORP (≥1200mV) oxidation potential and oxidizing power of this electrolyzed water rapidly decrease with time and temperature increases, leaving no residue. It exhibits advanced oxidizing characteristics of hydroxyl and oxygen free radicals. This high ORP electrolyzed water spray also releases negative air ions, but does not release detectable (>0.1mg / m³) ions into the surrounding environment. 3 The gaseous ozone produced meets environmental safety requirements. The key electrode (anode) of the novel water electrolysis device involves the use of wear-resistant, heat-resistant conductive diamond material. In this study, this novel water electrolysis device and water spray were used to treat wounds resulting from laser skin surgery.

[0139] This is an open-label, two-arm clinical trial (registered at www.clinicaltrials.com). The electrolyzed water spray group included 10 patients with mild discomfort and redness around wounds from laser skin surgery (0 males, 10 females, mean age 32 ± 8 years). Treatment involved spraying the wound area and surrounding tissue until 1 / 20 to 1 / 10 of the device's volume (20-40 ml) was used. The saline control group included 10 patients with mild discomfort and redness around wounds from laser skin surgery (0 males, 10 females, mean age 34 ± 7 years). Treatment involved spraying the affected area and surrounding tissue with saline solution until 1 / 20 to 1 / 10 of the control device's volume (20-40 ml) was used. After treatment, researchers followed up with patients and recorded the degree of skin discomfort one hour after treatment using a discomfort scale. This discomfort scale is a modification of the National Pain Rating Scale (NPRS), the most commonly used pain assessment scale in clinical practice, which doctors use to unilaterally assess the intensity of a patient's pain. Researchers used a number scale from 0 to 10 to represent patients' self-ratings of discomfort, with "0" indicating no discomfort and "10" indicating the most severe discomfort. In addition to assessing the degree of skin discomfort, researchers also measured the redness and bleeding of patients' wounds over 3 hours. Researchers used a number scale from 0 to 10 to represent the improvement in patients' wound redness and bleeding, with "0" indicating no redness or bleeding and "10" indicating the most severe redness and bleeding.

[0140] result:

[0141] Table 7-1. Discomfort around the wound caused by laser skin surgery.

[0142]

[0143] Table 7-2. Scale for Wound Improvement Caused by Laser Skin Surgery

[0144]

[0145] Discussion: 1. Indirect effects: Microbial endotoxins trigger excessive resistance from the human immune system, causing inflammation and leading to disease; oxidation reactions eliminate microorganisms and endotoxins, reducing inflammation; 2. Direct effects: The antipruritic reaction is rapid, occurring within 30 seconds after spraying; the inventors speculate that reactive oxygen free radicals in the body have certain immune and signal transduction functions or inactivate inflammatory mediators, inhibiting the inflammation that mediates itching.

[0146] Conclusion: This novel electrolyzed water spray significantly reduces skin discomfort and redness around the wound caused by laser skin surgery, with no observed adverse reactions. Water spray can also achieve similar effects.

[0147] Example 8

[0148] Objective: To conduct a clinical study on the reduction of genital itching in patients using a novel electrolyzed water spray or mist.

[0149] method:

[0150] This study used tap water and a novel water electrolysis device (CN215308550U) to instantly produce electrolyzed water. This electrolyzed water is highly electron-deficient and has a high oxidation potential (ORP) ≥1200mV. The resulting spray and water contain detectable levels of non-volatile oxidizing power equivalent to 2.18ppm-2.72ppm dissolved ozone (approximately 2.23ppm for spray and 2.67ppm for water). The high ORP (≥1200mV) oxidation potential and oxidizing power of this electrolyzed water rapidly decrease with time and temperature increases, leaving no residue. It exhibits advanced oxidizing characteristics of hydroxyl and oxygen free radicals. This high ORP electrolyzed water spray also releases negative air ions, but does not release detectable (>0.1mg / m³) ions into the surrounding environment. 3 The gaseous ozone produced meets environmental safety requirements. The key electrode (anode) of the novel water electrolysis device involves the use of wear-resistant, heat-resistant conductive diamond material. In this study, this novel water electrolysis device and water spray were used to treat wounds resulting from laser skin surgery.

[0151] This is an open-label, single-arm, pre- and post-treatment comparative study (NCT05766930). The study included 14 patients with genital pruritus (7 males, 7 females, mean age 52 ± 12 years). Treatment involved patients spraying the itchy areas of their genitals with a spray device twice daily for 5 days, filling half the device's capacity (250 ml). After treatment, researchers followed up with patients and recorded the severity of genital pruritus on days 1, 2, 3, 4, and 5 of treatment, using a pruritus scale. This pruritus scale was modified from the National Pain Rating Scale (NPRS), a commonly used clinical pain assessment scale used by physicians to unilaterally assess pain intensity. Researchers used a scale of 0-10 to represent patients' self-reported pruritus severity, with "0" indicating no pruritus and "10" indicating the most intense pruritus.

[0152] result:

[0153] Table 8-1. Patient Genital Itching Scale

[0154]

[0155] Discussion: 1. Indirect effects: Microbial endotoxins trigger excessive resistance from the human immune system, causing inflammation and leading to disease; oxidation reactions eliminate microorganisms and endotoxins, reducing inflammation; 2. Direct effects: The antipruritic reaction is rapid, occurring within 30 seconds after spraying; the inventors speculate that reactive oxygen free radicals in the body have certain immune and signal transduction functions or inactivate inflammatory mediators, inhibiting the inflammation that mediates itching.

[0156] Conclusion: This novel electrolyzed water spray significantly reduced the itching severity in patients with genital pruritus, with no observed adverse reactions. Spraying water can also achieve similar effects.

[0157] Example 9

[0158] Objective: To conduct a clinical study on the effect of a novel electrolyzed water spray on reducing scalp discomfort or itching.

[0159] method:

[0160] This study used tap water and a novel water electrolysis device (CN215308550U) to instantly produce electrolyzed water. This water is highly electron-deficient and has a high oxidation potential (ORP) ≥1200mV. The resulting spray and water, after spraying and pumping, contains detectable non-volatile oxidizing power equivalent to 2.23 ppm dissolved ozone. The high oxidation potential (≥1200mV) and oxidizing power of this high ORP electrolyzed water rapidly decrease with time and temperature increases, leaving no residue. It exhibits advanced oxidizing characteristics of hydroxyl and oxygen free radicals. This high ORP electrolyzed water spray also releases negative air ions, but does not release detectable (>0.1 mg / m³) ions into the surrounding environment. 3 The ozone gas produced meets environmental safety requirements. The key electrode (anode) of the novel water electrolysis device involves the use of wear-resistant, heat-resistant conductive diamond material. In this study, this novel water electrolysis device and water spray were used to treat scalp discomfort or itching.

[0161] This is an open-label, single-arm, pre- and post-treatment comparative study (NCT05765526). The study included 14 patients with scalp discomfort or itching (14 males, 0 females, mean age 42 ± 18 years). Treatment involved spraying the itchy areas of the scalp with a spray until half the device's volume (250 ml) was used, twice daily for 5 days. After treatment, researchers followed up with patients, recording the severity of scalp discomfort or itching on days 1, 2, 3, 4, and 5 of treatment using a discomfort and itching scale. This discomfort and itching scale was modified from the National Pain Rating Scale (NPRS), the most commonly used pain assessment scale in clinical practice, used by physicians to unilaterally assess the intensity of pain in patients. Researchers used numbers 0-10 to represent patients' self-ratings of scalp discomfort or itching, with "0" indicating no discomfort or itching and "10" indicating the most severe discomfort or itching.

[0162] result:

[0163] Table 9-1, Scalp Discomfort or Itching Scale

[0164]

[0165] Discussion: 1. Indirect effects: Microbial endotoxins trigger excessive resistance from the human immune system, producing inflammation and leading to disease; oxidation reactions eliminate microorganisms and endotoxins, reducing inflammation; 2. Direct effects: The antipruritic reaction is rapid, occurring within 30 seconds after spraying. We speculate that reactive oxygen species in the body have certain immune and signal transduction functions or inactivate inflammatory mediators, inhibiting the inflammation that mediates itching.

[0166] Conclusion: This novel electrolyzed water spray significantly reduced discomfort in patients with scalp irritation or itching, and no adverse reactions were observed.

[0167] Example 10

[0168] Objective: To study the reduction of Escherichia coli count and H1N1 influenza virus titer on human hands using novel electrolyzed water spray or water misting methods.

[0169] background:

[0170] The principle behind disinfectants killing viruses. Viruses can be inactivated by disinfectants primarily due to their own physicochemical properties. Structurally, most viruses can be divided into two categories: enveloped viruses (such as influenza and herpesviruses) and non-enveloped viruses (such as adenoviruses and enteroviruses). The biggest difference between the two types lies in whether the core structure of the virus, the protein capsid, is surrounded by an envelope. The main component of the envelope is lipids. Its presence enhances the virus's camouflage, making it easier for the virus to enter host cells and increasing structural stability. However, once enveloped viruses leave the host and are exposed to the external environment, their envelope is easily washed away and destroyed by lipid solvents, degreasing agents, strong alkalis, etc., becoming one of the main "targets" for disinfectants to kill the virus. Therefore, enveloped viruses are generally more sensitive to chemical disinfectants than non-enveloped viruses. In fact, not only ethanol, but most commonly used disinfectants on the market can rapidly inactivate viral variants.

[0171] Currently, commercially available disinfectants can be categorized into nine types based on their chemical composition: chlorine-based disinfectants (such as 84 disinfectant and bleaching powder), alcohol-based disinfectants (such as ethanol and isopropanol), peroxide-based disinfectants (such as peracetic acid, hydrogen peroxide, and hydrogen peroxide), aldehyde-based disinfectants (such as glutaraldehyde and formaldehyde), phenolic disinfectants (such as p-chloro-meta-phenol), ethylene oxide, biguanide disinfectants (such as chlorhexidine and chlorhexidine), quaternary ammonium salt disinfectants (such as benzalkonium bromide, benzalkonium chloride, and double-chain quaternary ammonium salts), and iodine-based disinfectants. Ethylene oxide and aldehyde disinfectants are generally used in professional settings such as hospitals and laboratories, while the remaining seven types are commonly used and readily available in our daily lives. These common household disinfectants vary in their effective action time, concentration, and sensitivity against viruses.

[0172] Chlorine-based disinfectants are highly effective at killing viruses, but as strong oxidants, they can corrode metals and bleach fabrics. Therefore, they are suitable for disinfecting surfaces such as tiles and plastics. Chlorine-based disinfectants should be prepared and used immediately, and gloves should be worn during use to prevent skin corrosion. After disinfection, the solution must be rinsed thoroughly with water. Furthermore, chlorine-based disinfectants should not be mixed with acidic cleaning products such as toilet cleaners, as they will react and produce toxic gases.

[0173] This study used tap water and a novel water electrolysis device (CN215308550U) to instantly produce electrolyzed water. This electrolyzed water is highly electron-deficient and has a high oxidation potential (ORP) ≥1200mV. The resulting spray and water contain detectable levels of non-volatile oxidizing power equivalent to 2.18ppm-2.72ppm dissolved ozone (approximately 2.23ppm for spray and 2.67ppm for water). The high ORP (≥1200mV) oxidation potential and oxidizing power of this electrolyzed water rapidly decrease with time and temperature increases, leaving no residue. It exhibits advanced oxidizing characteristics of hydroxyl and oxygen free radicals. This high ORP electrolyzed water spray also releases negative air ions, but does not release detectable (>0.1mg / m³) ions into the surrounding environment. 3 The ozone gas produced meets environmental safety requirements. The key electrode (anode) of the novel water electrolysis device utilizes wear-resistant and heat-resistant conductive diamond material.

[0174] method:

[0175] This is an open-label, two-arm clinical study (registered at www.clinicaltrials.com). The tap water electrolyzed spray group included 10 healthy participants (5 males, 5 females, mean age 32 ± 8 years). The treatment involved spraying pre-applied E. coli and attenuated human influenza virus H1N1 onto the hands for 6 seconds per hand, followed by 4 seconds to collect water samples from the sprayed hands and prepare a plating. The untreated tap water control group included 10 healthy participants (5 males, 5 females, mean age 32 ± 4 years). The treatment involved spraying untreated tap water pre-applied to the hands with either E. coli or human influenza virus H1N1 for 6 seconds per hand, followed by 4 seconds to collect water samples from the sprayed hands and prepare a plating.

[0176] The detection of residual E. coli in collected water samples included the following steps: 1 ml of the collected water sample was inoculated onto the surface of a solid culture medium (the content in the total collected water sample was then calculated). The sample was incubated overnight at 37°C. The total number of bacterial colonies formed was calculated, expressed as CFU. The total bacterial colony count (CFU) is the total number of colonies formed on the surface of 1 ml of water sample on the solid culture medium. Note: This experimental methodology assumes that bacteria inoculated on the hands in both experimental groups were washed off. The number of colonies remaining on the hands after washing is negligible due to the large number of colonies inoculated. After the experiment, fingerprints were pressed onto large agar plates using the washed hands. After overnight incubation at 37°C, the colonies (unstained and stained) were calculated, supporting the reliability of this experimental method.

[0177] The titer of residual human influenza virus H1N1 in collected water was determined using the following steps: The viral titer (TCID50) was determined using a 50% culture infectious dose of MDCK cells. MDCK cells were seeded at 1.0 × 10⁴ cells per well in a 96-well plate and incubated overnight at 37°C. Water samples collected after hand washing were serially diluted, and 100 μL of the sample was seeded per well with MDCK cells. After incubation at 37°C for 3 days, TCID50 was calculated using the Reed and Muench methods. Note: PFU (plaque forming unit) refers to the plaque formation of one virus particle and is used to describe the number of viruses. One PFU equals one virus particle.

[0178] result:

[0179] Table 10-1. Number of residual E. coli in collected water samples

[0180] Spray each hand for 6 seconds Electrolyzed water spray unit 0±0 Untreated tap water control group 130±36

[0181] Figure 1 This is a graph showing the viral titer (TCID50) of human influenza virus H1N1 in collected water samples.

[0182] Discussion: High-oxidizing potential water has strong oxidizing properties, which can oxidize and eliminate the "spikes" on the outer shell of viruses, causing them to lose their ability to bind to cells and infect. The professional sterilization rate reaches 99.999%, which can effectively kill a variety of common pathogens.

[0183] Conclusion: This high-oxidation-potential electrolyzed water spray significantly reduces the number of E. coli on human hands and the titer of human influenza virus H1N1. Spraying water can also achieve similar effects.

[0184] Example 11

[0185] Objective: To conduct a clinical study on the effect of novel electrolyzed water in relieving nasopharyngeal itching or discomfort.

[0186] method:

[0187] This study used physiological saline and a novel water electrolysis device (CN215308550U) to instantly produce electrolyzed water. This water is highly electron-deficient and has a high oxidation potential (ORP) ≥1200mV. The sprayed and pumped water contains detectable non-volatile oxidizing power equivalent to approximately 3.92 ppm of dissolved ozone. The high ORP electrolyzed water exhibits a rapid decay of its oxidation potential (≥1200mV) and oxidizing power with increasing temperature and time, leaving no residue. It possesses advanced oxidizing characteristics of hydroxyl and oxygen free radicals. This high ORP electrolyzed water spray also releases negative air ions, but does not release detectable (>0.1 mg / m³) ions into the surrounding environment. 3The ozone gas produced meets environmental safety requirements. The key electrode (anode) of the novel water electrolysis device utilizes a wear-resistant and heat-resistant conductive diamond material. In this study, this novel water electrolysis device and the electrolyzed water were used to treat nasal and pharyngeal itching or discomfort. Hydrolysis with physiological saline prevents nasal mucosal irritation, resulting in better efficacy for nasal itching.

[0188] This is an open-label, single-arm, pre- and post-treatment comparative study (registered at www.clinicaltrials.com). The treatment included 14 patients with concurrent nasal and pharyngeal itching or discomfort (7 males, 7 females, mean age 40 ± 15 years). Treatment involved rinsing the pharynx or nasal area with mouthwash or rinsing the nose until the device's 350 ml volume was used up, twice daily for 7 days. At the end of treatment, researchers followed up with patients and recorded nasal and pharyngeal itching or discomfort one hour after the first treatment, using an itching or discomfort scale. This scale was modified from the National Pain Rating Scale (NPRS), the most commonly used pain assessment scale in clinical practice, used by physicians to unilaterally assess the intensity of pain in patients. Researchers used a scale of 0-10 to represent patients' self-ratings of discomfort, with "0" indicating no itching or discomfort and "10" indicating the most intense itching or discomfort. In addition to assessing the degree of nasal and pharyngeal itching or discomfort, researchers also measured the degree of redness and swelling in the nasal cavity and pharynx on day 7 after the 7-day treatment period.

[0189] result:

[0190] Table 11-1, Scale for Nasopharyngeal Itching or Discomfort

[0191]

[0192]

[0193] Table 11-2 Scale for Improving the Degree of Redness and Swelling in the Nasal Cavity and Pharynx

[0194]

[0195] Discussion: 1. Indirect effects of electrolyzed water on the nasopharyngeal mucosa: Microorganisms and endotoxins trigger excessive resistance from the human immune system, causing inflammation and leading to disease; the oxidation reaction of electrolyzed water eliminates microorganisms and endotoxins, reducing inflammation of the nasopharyngeal mucosa; 2. Direct effects of electrolyzed water on the nasopharyngeal mucosa: The antipruritic reaction is rapid, occurring within 30 seconds after rinsing; the inventors speculate that reactive oxygen free radicals in the body have a certain immune signal transduction function or inactivate inflammatory mediators, directly inhibiting the inflammation that mediates itching.

[0196] Conclusion: This novel electrolyzed water significantly relieved nasal and pharyngeal itching or discomfort, as well as nasopharyngeal redness and swelling, with no adverse reactions observed.

[0197] Example 12

[0198] Objective: To conduct a clinical study on the effect of novel electrolyzed water spray or water mist on reducing inflammatory erythema in the diaper area.

[0199] method:

[0200] This study used tap water and a novel water electrolysis device (CN215308550U) to instantly produce electrolyzed water. This electrolyzed water is highly electron-deficient and has a high oxidation potential (ORP) ≥1200mV. The resulting spray and water contain detectable levels of non-volatile oxidizing power equivalent to 2.18ppm-2.72ppm dissolved ozone (approximately 2.23ppm for spray and 2.67ppm for water). The high ORP (≥1200mV) oxidation potential and oxidizing power of this electrolyzed water rapidly decrease with time and temperature, exhibiting advanced oxidizing characteristics of hydroxyl and oxygen free radicals. This high ORP electrolyzed water spray also releases negative air ions, but does not release detectable (>0.1mg / m³) ions into the surrounding environment. 3 The ozone gas produced meets environmental safety requirements. The key electrode (anode) of the novel water electrolysis device involves the use of wear-resistant and heat-resistant conductive diamond material. In this study, this novel water electrolysis device and the electrolyzed water were used to treat diaper dermatitis (red bottom). Diaper dermatitis (red bottom) manifests as erythema, papules, and small vesicular lesions in the diaper area; in severe cases, it can even lead to erosion and exudation.

[0201] This is an open-label, two-arm clinical study (registered at www.clinicaltrials.com). The electrolyzed water spray group included 10 patients with diaper rash (red bottom), 3 males and 7 females, with a mean age of 1.5 ± 0.2 years. Treatment involved spraying the wound area and surrounding tissue until 1 / 20 to 1 / 10 of the device's volume (20-40 ml) was used. The saline control group included 10 patients with diaper rash (red bottom), 4 males and 6 females, with a mean age of 1.4 ± 0.3 years. Treatment involved spraying the affected area and surrounding tissue with saline until 1 / 20 to 1 / 10 of the control device's volume (20-40 ml) was used. After treatment, researchers followed up with the patients' mothers, recording their feedback on the erythema in the diaper area one hour after treatment. Researchers used a scale of 0-10 to represent the severity of erythema, with "0" indicating no erythema and "10" indicating the most severe erythema.

[0202] result:

[0203] Table 12-1 Scale for Improvement of Skin Erythema in Diaper Area

[0204]

[0205] Discussion: 1. Indirect effects: Microbial endotoxins trigger excessive resistance from the human immune system, causing inflammation and leading to disease; oxidation reactions eliminate microorganisms and endotoxins, reducing inflammation; 2. Direct effects: The antipruritic reaction is rapid, occurring within 30 seconds after spraying; the inventors speculate that reactive oxygen free radicals in the body have certain immune and signal transduction functions or inactivate inflammatory mediators, inhibiting the inflammation that mediates itching.

[0206] Conclusion: This novel electrolyzed water spray significantly reduced the degree of erythema in the diaper area, and no adverse reactions were observed. Water spray can also achieve similar effects.

[0207] In summary, the electrolyzed water in Examples 4-12 exhibits significant alleviating or therapeutic effects on mild senile groin eczema, neurodermatitis and psoriasis, mild dermatitis, mild periodontitis, inflammatory wounds resulting from laser skin surgery, genital and scalp itching, and the elimination of bacteria and viruses on hands or skin. However, the key to generating this electrolyzed water lies in the crucial anode portion, which involves the use of a conductive diamond material layer. Therefore, this invention is not limited to the specific device described above. Any electrolyzed water spray or mist produced instantly using an electrolyzed water device with a conductive diamond material layer on the anode surface possesses a high oxidation potential of ≥1200mV. Simultaneously, a non-volatile oxidizing capacity equivalent to 2.18ppm-4.22ppm of dissolved ozone can be detected using an iodometric dissolved ozone meter, without releasing detectable levels of ≥0.1mg / m³ into the surrounding environment. 3 The gaseous ozone; this instant-generated spray or water spray can be used to relieve or treat inflammatory diseases of the skin and mucous membranes. The electrolysis feedstock used in Examples 4-12 above is tap water. Of course, as can be seen from Examples 1-3, pure water, saline solution, and mineral water can all achieve similar effects in relieving or treating inflammatory diseases of the skin and mucous membranes.

[0208] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Any simple modifications, equivalent changes, or alterations made by those skilled in the art using the disclosed technical content shall fall within the protection scope of the present invention.

Claims

1. A novel application of water electrolysis, characterized in that: The electrolyzed water is an electrolyzed water spray or mist produced instantly using an electrolysis device. It has a high oxidation potential of ≥1200mV but does not release detectable levels of ≥0.1mg / m³ into the surrounding environment. 3 The gaseous ozone; the high oxidation potential of ≥1200mV decays rapidly with time and temperature. The anode surface of the water electrolysis device is made using a layer of conductive diamond material. The electrolytic raw material for the water electrolysis device is physiological saline. The new applications are specifically: direct use to relieve or treat inflammatory diseases of the skin and mucous membranes; or for the preparation of drugs to relieve or treat inflammatory diseases of the skin and mucous membranes.

2. The novel application of water electrolysis according to claim 1, characterized in that, Electrolyzed water spray or water jet contains detectable non-volatile oxidizing power equivalent to 3.62 ppm to 4.22 ppm of dissolved ozone; said oxidizing power decays rapidly over time and with increasing temperature.

3. The novel application of water electrolysis according to claim 2, characterized in that, The electrolyzed water spray or mist has a pH of 7.6-8.6, which is between neutral and slightly alkaline, and also releases negative air ions.

4. The new application of water electrolysis according to claim 1, characterized in that, The water electrolysis device is a low-pressure water electrolysis device.

5. A novel application of water electrolysis according to any one of claims 1-4, characterized in that, The inflammatory diseases of the skin and mucous membranes are characterized by itchy skin or red and swollen skin.

6. The novel application of water electrolysis according to claim 5, characterized in that, The specific symptoms of skin itching or redness and swelling are as follows: (1) Itching or chronic skin and mucous membrane inflammation in elderly patients with groin eczema, neurodermatitis or psoriasis; (2) Skin trauma caused by itching on the soles of the feet or athlete's foot; (3) Patients with periodontitis experience gum discomfort or red, swollen, and bleeding gums; (4) Redness and swelling of the skin around the wound caused by laser skin surgery; (5) Perineal itching; (6) Scalp discomfort or itching; (7) The number of E. coli on a person’s hands or the titer of human influenza virus H1N1; (8) Nasopharyngeal itching or discomfort; Or (9) Inflammatory erythema of the diaper area.

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