Crop total-effect biostimulant based on mineral substance-enzyme composite technology and preparation method of crop total-effect biostimulant

Abstract

The invention provides a crop total-effect biostimulant based on a mineral substance-enzyme composite technology and a preparation method of the crop total-effect biostimulant. The composition is prepared from a phase A, a phase B, a phase C and a phase D, wherein the phase A is prepared from full-spectrum mineral chelated microelement liquid, macroelement nitrogen, phosphorus and potassium: 50 kg of potassium nitrate, 20 kg of ammonium dihydrogen phosphate, medium element: 30 kg of magnesium sulfate heptahydrate, full-spectrum microelement: 5.0 kg of iron: EDTA-Fe: 3.0 kg of manganese: EDTA-Mn: 2.5 kg of zinc: EDTA-Zn: 1.0 kg of EDTA-Cu: 2.0 kg of boric acid: 0.5 kg of molybdenum: ammonium molybdate: 0.1 kg of cobalt chloride: 0.05 kg of selenium: sodium selenite: 8.0 kg of silicon: potassium silicate: 0.8 kg of titanium: citric acid titanium: rare earth; the preparation method comprises the following steps: dissolving the components in 600L of pure water, and fully stirring until the components are completely dissolved to obtain a phase A; according to the crop total-effect biostimulant based on the mineral substance-enzyme composite technology and the preparation method of the crop total-effect biostimulant, the crop nutrition and physiological problems can be solved, long-term coexistence of high-concentration mineral substances and enzyme activity is ensured, the crop stress resistance is improved, physiological diseases are prevented and treated, and the crop yield and quality are improved.

Description

Technical Field

[0001] This invention relates to the fields of agricultural chemistry and plant nutrition, and more specifically, to a crop-based all-purpose biostimulant based on mineral-enzyme complex technology and its preparation method. Background Technology

[0002] In modern intensive agriculture, crops generally face hidden hunger, namely micronutrient deficiency, as well as various biotic and abiotic stresses (drought, salinity, and diseases, etc.), resulting in low fertilizer utilization, premature aging, reduced yield, and decreased quality.

[0003] Existing technologies have the following shortcomings: 1. Traditional micronutrient fertilizers are mostly in the form of inorganic salts, which are easily fixed by the soil, have low absorption rates, and exhibit antagonism between elements. Mixing them can easily lead to precipitation, making it difficult to achieve comprehensive supplementation; 2. Ordinary foliar fertilizers or biostimulants have single functions, focusing either on providing nutrition or on regulating growth. They lack a solution that systematically combines mineral nutrition with enzyme-catalyzed metabolism, making it impossible to achieve bidirectional regulation of crop physiological activities; 3. For systemic problems such as premature crop aging, nutrient deficiency syndrome, and soil-borne diseases, single products are often insufficient to solve complex problems. Farmers need to purchase multiple products for compounding, increasing costs and operational complexity. Improper mixing can also easily cause phytotoxicity.

[0004] Therefore, there is an urgent need in this field for a comprehensive solution that can organically combine full-spectrum minerals in a highly active form with key metabolic enzyme systems to fundamentally activate the crop's own potential and achieve dual enhancement of nutrition and immunity. Summary of the Invention

[0005] The present invention aims to solve the technical problems mentioned in the background art above, and to provide a crop all-purpose biostimulant based on mineral-enzyme complex technology and its preparation method.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a crop-based all-purpose biostimulant based on mineral-enzyme composite technology, wherein the composition formulation, dosage, and steps for preparing 1000L of finished product are as follows: Phase A: Full-spectrum mineral chelated trace element solution Macronutrients nitrogen, phosphorus, and potassium: 50 kg potassium nitrate, 20 kg ammonium dihydrogen phosphate Medium element: Magnesium sulfate heptahydrate 30kg Full spectrum trace elements: Iron: EDTA-Fe 5.0kg Manganese: EDTA-Mn 3.0kg Zinc: EDTA-Zn 2.5kg Copper: EDTA-Cu 1.0kg Boron: Boric acid 2.0 kg Molybdenum: Ammonium molybdate 0.5 kg Cobalt: 0.1 kg cobalt chloride Selenium: Sodium selenite 0.05kg Silicon: Potassium silicate 8.0 kg Titanium: Titanium citrate 0.8kg Rare earth: 1.5 kg of rare earth nitrate Dissolve the above components in 600L of pure water and stir thoroughly until completely dissolved to obtain phase A; Phase B: Complex enzyme and organic carrier solution Complex enzyme preparation: Superoxide dismutase activity: 10 million units Peroxidase activity: 8 million units Chitinase activity: 5 million units Cellulase activity: 3 million units Natural organic acid chelating agent and stabilizer: Humic acid concentrate: 50kg Seaweed extract concentrate powder: 10kg Polyaspartic acid: 5kg Natural pigments and antioxidants: Sodium copper chlorophyllin: 1.5kg L-Ascorbic Acid: 2.0kg Dissolve the above ingredients in 400L of pure water and stir slowly at low speed to avoid damaging enzyme activity, thus obtaining phase B.

[0007] A further preferred embodiment: the rare earth nitrate includes Ce and La, and the humic acid concentrate is a fulvic acid concentrate.

[0008] A method for preparing a crop-based all-purpose biostimulant based on mineral-enzyme complex technology as described above includes the following steps: a. Separate preparation: Prepare phase A and phase B separately at room temperature (20-25℃); b. Slow mixing: While stirring at low speed, slowly pour the liquid of phase A into the liquid of phase B; c. Maturation and volume adjustment: After mixing, stir continuously at low speed for at least 2 hours to allow it to fully complex and mature. Finally, add pure water to make the final total volume 1000L, and control the liquid specific gravity at 1.18-1.22. d. Filtration and filling: The liquid is filtered through a 1μm precision filter to ensure it is clear and transparent, and then immediately packaged in a light-proof manner. Beneficial effects

[0009] This crop-based all-purpose biostimulant and its preparation method, based on mineral-enzyme composite technology, innovatively combines full-spectrum chelated minerals with key stress-resistance enzymes (SOD, POD) and antibacterial enzymes (chitinase). It provides comprehensive nutrition while systematically enhancing the crop's antioxidant and disease-resistant capabilities, addressing premature aging from within. Through multiple chelations using humic acid, polyaspartic acid, and other ingredients, it ensures that extremely high concentrations of mineral elements exist stably in a form directly absorbable by plants, achieving an absorption rate several times higher than traditional products. The introduction of rare functional elements further enhances its efficacy. Broad-spectrum and highly effective, this multi-functional product prevents nutrient deficiencies and provides a comprehensive and balanced supply of trace elements. (SOD...) The / POD enzyme system directly eliminates excess free radicals generated by stress, enhancing crops' resistance to drought, low temperatures, and salinity. Chitinase can decompose the cell walls of pathogens (such as fungi), while cellulase can improve cell wall permeability and promote absorption, providing good adjunctive treatment for soil-borne diseases. By improving photosynthesis (chlorophyll precursors), enhancing photosynthetic efficiency, increasing fruit set rate, and promoting the accumulation of dry matter and effective components (such as sugars and proteins), yield can be increased by more than 20%. The dark green appearance and color-changing characteristic upon exposure to light are not only the result of stable technology but also constitute a built-in, intuitive product quality and authenticity identification system, making it highly unique in the market. Detailed Implementation

[0010] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention.

[0011] In this embodiment of the invention, a crop-based all-purpose biostimulant based on mineral-enzyme composite technology is prepared. The composition formulation, dosage, and steps are as follows for each 1000L of finished product: Phase A: Full-spectrum mineral chelated trace element solution Provides a large amount of nitrogen, phosphorus, and potassium: 50 kg of potassium nitrate and 20 kg of ammonium dihydrogen phosphate. Provides core medium element: 30kg magnesium sulfate heptahydrate Provides full spectrum of trace elements: Iron (Fe): EDTA-Fe 5.0kg Manganese (Mn): EDTA-Mn 3.0kg Zinc (Zn): EDTA-Zn 2.5kg Copper (Cu): EDTA-Cu 1.0kg Boron (B): Boric acid 2.0 kg Molybdenum (Mo): Ammonium molybdate 0.5 kg Cobalt (Co): 0.1 kg cobalt chloride Selenium (Se): Sodium selenite 0.05kg Silicon (Si): Potassium silicate 8.0 kg (strengthens cell walls, resists lodging, deposits on cell walls to form silicified cells, physically enhances crop resistance to pests and diseases and lodging resistance) Titanium (Ti): Titanium citrate 0.8 kg (a catalyst to improve photosynthetic efficiency, which can significantly increase chlorophyll content and photosynthetic rate, promote nitrogen metabolism, and is a catalyst for increasing crop yield and quality) Rare Earth (Re): Rare Earth Nitrate (mainly Ce and La) 1.5kg (Broad-spectrum growth regulator. As a broad-spectrum plant growth regulator, it can activate the activity of various enzymes, promote seed germination and root development, enhance the crop's ability to adapt to adversity, and is the nemesis of acclimatization problems). Dissolve the above components in 600L of pure water and stir thoroughly until completely dissolved to obtain phase A; Phase B: Complex enzyme and organic carrier solution Complex enzyme preparation (functional engine): Superoxide dismutase (SOD) activity: 10 million units Peroxidase (POD) activity: 8 million units Chitinase activity: 5 million units Cellulase activity: 3 million units Natural organic acid chelating agent and stabilizer: Humic acid (fulvic acid) concentrate: 50kg Seaweed extract concentrate powder: 10kg Polyaspartic acid (high molecular weight chelating agent): 5kg Natural pigment and antioxidant: Sodium copper chlorophyllin: 1.5kg (provides and stabilizes dark green color) L-Ascorbic Acid (Vitamin C): 2.0 kg Dissolve the above ingredients in 400L of pure water and stir slowly at low speed to avoid damaging enzyme activity, thus obtaining phase B.

[0012] Among these measures, trace element substitutions include: EDTA chelating agents can be partially replaced with novel chelating agents such as EDDHA and IDHA, especially for iron. Rare earth elements can be replaced with rare earth chlorides or amino acid rare earths instead of rare earth nitrates. Enzyme preparation expansion: β-1,3-glucanase and chitinase can be added for synergistic antibacterial effects, or glutathione peroxidase can be added to further enhance antioxidant capacity. Organic carrier substitutions: Seaweed extracts can be partially or completely replaced with amino acid concentrates, fish protein hydrolysates, and other natural biostimulant sources. Color adjustment: Sodium copper chlorophyll can be replaced with other safe natural green pigments, or the dosage can be adjusted to control the depth of green, but its light-sensitive color-changing property should be retained.

[0013] A method for preparing a crop-based all-purpose biostimulant, as described above, based on mineral-enzyme composite technology, includes the following steps: a. Separate preparation: Prepare phase A and phase B separately at room temperature (20-25℃); b. Slow mixing: While stirring at low speed, slowly pour the liquid of phase A into the liquid of phase B; c. Maturation and volume adjustment: After mixing, continue stirring at low speed for at least 2 hours to allow it to fully complex and mature. Finally, add pure water to make the final total volume 1000L, and control the liquid specific gravity at 1.18-1.22 (i.e., 1:1.2). d. Filtration and filling: The liquid is filtered through a 1μm precision filter to ensure it is clear and transparent, and then immediately packaged in a light-proof manner.

[0014] Regarding product properties and storage: The product appears as a clear, dark green liquid. Its stability is maintained when stored away from light. However, upon exposure to light, due to the photolysis of chlorophyll derivatives and changes in the valence state of trace elements (especially rare earth elements and titanium, and hydrolysis), the liquid will gradually change from dark green to brownish-yellow, and finally to brownish-red. This color change serves as a direct visual indicator of whether the product has become damaged by light exposure.

Claims

1. A crop-based all-purpose biostimulant based on mineral-enzyme complex technology, characterized in that: For each 1000L of finished product prepared, the composition formulation, dosage, and steps are as follows: Phase A: Full-spectrum mineral chelated trace element solution Macronutrients nitrogen, phosphorus, and potassium: 50 kg potassium nitrate, 20 kg ammonium dihydrogen phosphate Medium element: Magnesium sulfate heptahydrate 30kg Full spectrum trace elements: Iron: EDTA-Fe 5.0kg Manganese: EDTA-Mn 3.0kg Zinc: EDTA-Zn 2.5kg Copper: EDTA-Cu 1.0kg Boron: Boric acid 2.0 kg Molybdenum: Ammonium molybdate 0.5 kg Cobalt: 0.1 kg cobalt chloride Selenium: Sodium selenite 0.05kg Silicon: Potassium silicate 8.0 kg Titanium: Titanium citrate 0.8kg Rare earth: 1.5 kg of rare earth nitrate Dissolve the above components in 600L of pure water and stir thoroughly until completely dissolved to obtain phase A; Phase B: Complex enzyme and organic carrier solution Complex enzyme preparation: Superoxide dismutase activity: 10 million units Peroxidase activity: 8 million units Chitinase activity: 5 million units Cellulase activity: 3 million units Natural organic acid chelating agent and stabilizer: Humic acid concentrate: 50kg Seaweed extract concentrate powder: 10kg Polyaspartic acid: 5kg Natural pigments and antioxidants: Sodium copper chlorophyllin: 1.5kg L-Ascorbic Acid: 2.0kg Dissolve the above ingredients in 400L of pure water and stir slowly at low speed to avoid damaging enzyme activity, thus obtaining phase B.

2. The crop-based all-purpose biostimulant based on mineral-enzyme composite technology according to claim 1, characterized in that: The rare earth nitrates include Ce and La, and the humic acid concentrate is a fulvic acid concentrate.

3. A method for preparing a crop-based all-purpose biostimulant based on mineral-enzyme composite technology as described in any one of claims 1-2, characterized in that: Includes the following steps: a. Separate preparation: Prepare phase A and phase B separately at room temperature (20-25℃); b. Slow mixing: While stirring at low speed, slowly pour the liquid of phase A into the liquid of phase B; c. Maturation and volume adjustment: After mixing, stir continuously at low speed for at least 2 hours to allow it to fully complex and mature. Finally, add pure water to make the final total volume 1000L, and control the liquid specific gravity at 1.18-1.

22. d. Filtration and filling: The liquid is filtered through a 1μm precision filter to ensure it is clear and transparent, and then immediately packaged in a light-proof manner.