Measurement module and chemiluminescence immunoassay analyzer

Abstract

The present invention discloses a measurement module and a chemiluminescence immunoassay analyzer, and belongs to the technical field of chemiluminescence immunoassay, the measurement module comprises a measurement part, the measurement part comprises an analyzer main body, an injection assembly for injecting a tracer substance is arranged in the analyzer main body, and a first chute and a second chute are arranged in the analyzer main body, by arranging the micro-magnetic shielding sheet, the magnetic field is weakened to prevent the adsorbed magnetic beads from colliding with the wall, then the weakening is relieved to gather the magnetic beads, and finally the magnetic beads are weakened again to be flatly laid, so that dynamic adjustment is realized, and center gathering of the magnetic beads without deviation, wall attachment and edge accumulation is realized through a symmetric gradient weak magnetic field formed by the main magnetic block and the annular magnetic block; and finally, the shielding sheet is used for softening a central peak magnetic field and eliminating a magnetic field interference dead zone, so that the magnetic beads form a uniform tiled state, the conditions that light emitted by the surfaces of the magnetic beads is mutually shielded and signal acquisition errors are caused due to distribution deviation, wall attachment or edge accumulation of the magnetic beads are avoided, and the detection stability and accuracy are improved.

Description

Technical Field

[0001] This invention relates to the field of chemiluminescence immunoassay technology, and more specifically, to a measurement module and a chemiluminescence immunoassay analyzer. Background Technology

[0002] The core principle of chemiluminescence immunoassay is based on the specific immune binding reaction between antigen and antibody. Superparamagnetic beads are used as carriers of immune complexes. Specific antibodies / antigens immobilized on the surface of the magnetic beads capture the analyte in the sample. Then, a detection reagent labeled with a tracer luminescent substance (such as acridinium ester, alkaline phosphatase, horseradish peroxidase, etc.) is combined to form a "magnetic bead-analyte-luminescent label" immune complex. Subsequently, a chemiluminescent substrate is added to trigger the luminescence reaction, and the light signal intensity is collected by a photon counter (PMT), thereby realizing the quantitative analysis of the analyte concentration.

[0003] The measurement module, as the core signal acquisition unit of the chemiluminescence immunoassay analyzer, plays a crucial role in achieving precise positioning of the immune complex magnetic beads and efficient capture of the light signal. The distribution of the magnetic beads (e.g., whether they are offset, adhered to the wall, stacked, or adhered to the bottom), the sufficiency of the luminescence reaction, and the purity of the detection environment directly determine the accuracy and stability of the light signal acquisition, making it a key factor affecting the overall detection performance of the instrument. However, existing chemiluminescence immunoassay analyzers still have several technical shortcomings in practical applications, as detailed below:

[0004] The magnetic fields of existing measurement modules are mostly statically designed, which cannot dynamically adjust the magnetic field strength and distribution according to the needs of different stages such as reaction cup transfer, substrate addition, and detection acquisition. During detection, either the magnetic field strength is insufficient, causing the magnetic beads to shift, stick to the wall or accumulate at the edge, or the central magnetic field peak is too strong, causing the magnetic beads to be over-focused and stacked. Abnormal distribution of magnetic beads will directly cause the light-emitting surfaces of the magnetic beads to block each other, resulting in optical signal acquisition errors and seriously affecting the stability and accuracy of detection.

[0005] In view of this, we propose a measurement module and a chemiluminescence immunoassay analyzer. Summary of the Invention

[0006] Technical problem to be solved: The purpose of this invention is to provide a measurement module and a chemiluminescence immunoassay analyzer, which solves the technical problems mentioned in the background art.

[0007] Technical solution: The technical solution of the present invention provides a measurement module and a chemiluminescence immunoassay analyzer, including a measurement component, which includes an analyzer body. The analyzer body has an injection component for injecting tracer substances inside, a first slide and a second slide inside, and a measurement component for detecting chemiluminescence signals in a reaction vessel inside. The tiling component includes an adjustment assembly disposed within the analyzer body for adjusting magnetic beads within the reaction vessel. The adjustment assembly contains a shielding assembly for weakening the magnetic field of the main magnetic block. The analyzer body contains a transmission assembly for adjusting the shielding assembly.

[0008] As an optional solution to the technical solution of this invention document, the analyzer body includes a main body shell, a feed trough is provided on the side of the main body shell, a conveyor belt is provided inside the feed trough, a plurality of slots are provided on the conveyor belt, a locking block is engaged inside the slot, and a reaction cup is provided on the side of the locking block. The conveyor belt passes through the feed trough, the size of the reaction cup is adapted to the size of the feed trough, the reaction cup is located inside the feed trough, and a first sliding groove is opened inside the main body shell, the first sliding groove is located above the feed trough.

[0009] As an optional solution to the technical solution of this invention, the injection assembly includes a motor disposed on the top of the main body shell, a lead screw disposed below the motor, a slide plate threaded onto the lead screw, and an injection head disposed at the bottom of the slide plate; The lead screw extends through the top of the main body shell to the inside of the first slide groove. The injection head is located on the side of the lead screw. The lead screw is slidably connected to the inner wall of the first slide groove. The bottom of the injection head extends through the inner wall of the first slide groove to the inside of the feed groove. The injection head is located directly above the reaction cup.

[0010] As an optional solution to the technical solution of this invention, the measuring component includes a first connecting plate fixedly connected to the side of the slide plate, a first rack fixedly connected to the bottom of the first connecting plate, a hollow groove formed on the first rack, a drive gear meshing with the side of the first rack, a bevel gear fixedly connected to the side of the drive gear, a transmission gear disposed below the drive gear, a second rack meshing with the side of the transmission gear, a light shield fixedly connected to the bottom of the second rack, and a photon counter disposed inside the light shield.

[0011] As an optional solution to the technical solution of this invention, the first connecting plate is located on the side of the injection head away from the lead screw, the transmission gear is located above the light shield, a bevel gear is fixedly connected to the top of the transmission gear, the two bevel gears mesh with each other, the first rack is located on the side of the light shield away from the injection head, the second rack passes through the hollow groove, the second rack is slidably connected to the hollow groove, the first connecting plate is slidably connected to the first sliding groove, the inside of the feed groove is provided with a second sliding groove, the light shield is slidably connected to the second sliding groove, and the second rack extends through the inner wall of the second sliding groove to the outer side of the main body shell.

[0012] By adopting the above technical solution, the measurement components can be isolated from external stray light.

[0013] As an optional solution to the technical solution of this invention, the adjustment component includes a cylinder disposed inside the main body shell, a telescopic rod disposed inside the cylinder, an adjustment shell fixedly connected to the top of the telescopic rod, an annular magnetic block and a main magnetic block disposed inside the adjustment shell, and an adjustment groove opened inside the main body shell; The main magnetic block is located at the center of the annular magnetic block, the adjusting groove is located below the feeding groove, the adjusting groove is connected to the feeding groove, and the adjusting shell is slidably connected to the inner wall of the adjusting groove.

[0014] By adopting the above technical solution, the adjustment component can be set to adjust the strongest focusing surface of the magnetic field.

[0015] As an optional solution to the technical solution of this invention, the shielding component includes two third sliding grooves opened inside the adjusting shell. A second connecting plate is slidably connected inside the third sliding groove. A micro magnetic shielding sheet is fixedly connected to one side of each of the two second connecting plates that are close to each other. A first elastic element and an air bladder are provided inside the third sliding groove. A ventilation groove is opened inside the adjusting shell. Corrugated pipes are provided on both sides of the adjusting shell.

[0016] As an optional solution to the technical solution of this invention, two of the micro-magnetic shielding sheets are combined to form a shielding plate. The cross-section of the main magnetic block is the same as that of the shielding plate. The shielding plate is located directly above the main magnetic block. The second connecting plate is elastically connected to the inner wall of the third sliding groove through a first elastic element. The open end of the airbag is fixedly connected to the inner wall of the third sliding groove. The airbag is located on the side of the second connecting plate away from the micro-magnetic shielding sheet. The side of the airbag close to the second connecting plate is fixedly connected to the second connecting plate. The first elastic element is located between the micro-magnetic shielding sheet and the airbag. The interior of the corrugated pipe communicates with the interior of the airbag through a ventilation groove. The end of the corrugated pipe away from the ventilation groove is fixedly connected to the inner wall of the adjustment groove.

[0017] By adopting the above technical solution, the shielding component can soften and weaken the central peak magnetic field of the main magnetic block.

[0018] As an optional solution to the technical solution of this invention, the transmission assembly includes a pressure plate fixedly connected to the side of the slide away from the first connecting plate, and two cavities opened in the main body shell. A first trigger plate is provided above the pressure plate, and a second trigger plate is provided below the pressure plate. A piston rod is slidably connected inside the cavity, and a second elastic element is provided inside the cavity. An air intake groove, an air inflation groove, and a flow diversion groove are opened inside the main body shell.

[0019] As an optional solution to the technical solution of this invention, the pressure plate is slidably connected to the inner wall of the first slide groove, the bottom of the first trigger plate abuts against the top of the pressure plate, the piston rod is elastically connected to the inner wall of the cavity through the second elastic element, the end of the piston rod away from the second elastic element extends through the inner wall of the cavity into the interior of the first slide groove, the sides of the first trigger plate and the second trigger plate that are away from each other are fixedly connected to the piston rod, the side of the first trigger plate is provided with an air intake groove, the side of the second trigger plate is provided with an air inflation groove, the two cavities are respectively connected to the interior of the diversion groove through the air intake groove and the air inflation groove, and the air intake groove and the air inflation groove are both connected to the bellows through the diversion groove.

[0020] Beneficial effects: One or more technical solutions provided in this invention have at least the following technical effects or advantages: 1. By setting up a micro-magnetic shielding sheet, the magnetic field is weakened during the transport of the reaction cup to prevent the magnetic beads from hitting the walls. During the feeding process, the weakening is released to gather the magnetic beads together. Finally, the magnetic field is weakened again between detections to make the magnetic beads spread out evenly. This achieves dynamic adjustment. Through the symmetrical gradient weak magnetic field formed by the main magnetic block and the ring magnetic block, the magnetic beads are centrally gathered without shifting, sticking to the walls, or accumulating at the edges. Finally, the shielding sheet softens the central peak magnetic field, making the magnetic beads form a uniform and flat state. This avoids the situation where the surface light of the magnetic beads is mutually blocked and the signal acquisition error occurs due to the magnetic beads being offset, sticking to the walls, or accumulating at the edges, thus improving the stability and accuracy of the detection.

[0021] 2. During the process of weakening and aggregating the magnetic beads as described above, the superparamagnetic beads will move simultaneously in the reaction vessel, slowly disturbing the liquid in the reaction vessel, increasing the contact area between the substrate and the tracer luminescent material on the surface of the magnetic beads, promoting the full chemiluminescence reaction, making the light signal intensity more matched with the concentration of the analyte, and further improving the accuracy of the detection results.

[0022] 3. By adjusting the cooperation between the outer shell and the telescopic rod, the gap between the main magnetic block and the outer wall of the bottom of the reaction cup is precisely controlled at 0.5 to 2 mm. The strongest focusing surface of the magnetic field is raised to the liquid phase area 1 to 3 mm above the bottom of the cup, so that the magnetic beads are suspended inside the liquid. This avoids the problem of light path obstruction and uneven light emission caused by the magnetic beads adhering to the bottom, and ensures that the light-emitting surface of the magnetic beads is fully exposed.

[0023] 4. By synchronously linking the light shield with the injection head, the light shield adheres to the surface of the reaction cup in time when the substrate is added to form a closed detection space, which isolates external stray light and provides a pure detection environment for the photon counter, avoiding signal acquisition errors caused by stray light and ensuring the accurate capture of weak chemiluminescence signals.

[0024] 5. At the same time, the horizontal optical path of the photon counter is precisely aligned with the magnetic bead levitation layer. Combined with the advantage of the single-layer flat magnetic bead without lateral obstruction, the optical signal can be collected without loss or interference, which greatly improves the efficiency and integrity of optical signal collection and further enhances the detection effect. Attached Figure Description

[0025] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the following description of the embodiments will be briefly introduced. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0026] Figure 1 This is a three-dimensional structural diagram of the measurement module and the chemiluminescence immunoassay analyzer.

[0027] Figure 2 This is a cross-sectional structural diagram of the measurement module and the main body of the analyzer in the chemiluminescence immunoassay analyzer.

[0028] Figure 3 For measurement modules and chemiluminescence immunoassay analyzers Figure 2 Enlarged structural diagram at point A in the middle.

[0029] Figure 4 For measurement modules and chemiluminescence immunoassay analyzers Figure 2 Enlarged structural diagram at point B.

[0030] Figure 5 This is a cross-sectional schematic diagram of the measurement module and the measurement components in the chemiluminescence immunoassay analyzer.

[0031] Figure 6 This is a schematic diagram showing the structural relationship between the light shield and the second slide in the measurement module and the chemiluminescence immunoassay analyzer.

[0032] Figure 7 This is a cross-sectional schematic diagram of the measurement module and the adjustment components in the chemiluminescence immunoassay analyzer.

[0033] Figure 8 This is a cross-sectional schematic diagram of the shielding components in the measurement module and chemiluminescence immunoassay analyzer.

[0034] Figure 9 This is a three-dimensional structural diagram of the measurement module and the measurement components in the chemiluminescence immunoassay analyzer.

[0035] Figure 10 This is a three-dimensional structural diagram of the shielding components in the measurement module and chemiluminescence immunoassay analyzer.

[0036] Figure 11This is a schematic diagram showing the structural relationship and coordination between the main magnetic block and the micro-magnetic shielding sheet in the measurement module and chemiluminescence immunoassay analyzer.

[0037] The following are the labeling instructions in the diagram: 10. Analyzer body; 101. Body shell; 102. Feed chute; 103. Conveyor belt; 104. Slot; 105. Block; 106. Reaction cup; 11. Injection assembly; 111. Motor; 112. Lead screw; 113. Slide plate; 114. Injection head; 12. First slide; 13. Measuring assembly; 131. First connecting plate; 132. First rack; 133. Hollowed-out groove; 134. Drive gear; 135. Bevel gear; 136. Transmission gear; 137. Second rack; 138. Light shield; 139. Photon counter; 14. Second slide.

[0038] 20. Adjustment assembly; 201. Cylinder; 202. Telescopic rod; 203. Adjustment housing; 204. Annular magnetic block; 205. Main magnetic block; 206. Adjustment groove; 21. Shielding assembly; 211. Third slide groove; 212. Second connecting plate; 213. Micro-magnetic shielding sheet; 214. First elastic element; 215. Airbag; 216. Ventilation groove; 217. Bellows; 22. Transmission assembly; 221. Pressure plate; 222. First trigger plate; 223. Second trigger plate; 224. Cavity; 225. Piston rod; 226. Second elastic element; 227. Intake groove; 228. Inflation groove; 229. Diversion groove. Detailed Implementation

[0039] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0040] In the description of this invention, it should be understood that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0041] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or a link; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal communication between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0042] Reference Figures 1 to 11 The present invention provides a measurement module and a chemiluminescence immunoassay analyzer, including a measurement component, which includes an analyzer body 10, an injection component 11 for injecting tracer substances is provided inside the analyzer body 10, a first slide 12 and a second slide 14 are provided inside the analyzer body 10, and a measurement component 13 for detecting chemiluminescence signals in a reaction cup 106 is provided inside the analyzer body 10.

[0043] The flattening component includes an adjustment assembly 20 disposed within the analyzer body 10 for adjusting the magnetic beads within the reaction cup 106. The adjustment assembly 20 is provided with a shielding assembly 21 for weakening the magnetic field of the main magnetic block 205. The analyzer body 10 is provided with a transmission assembly 22 for adjusting the shielding assembly 21.

[0044] Reference Figure 1 , Figure 2 and Figure 5 The present invention provides a measurement module and a chemiluminescence immunoassay analyzer. The analyzer body 10 includes a main body shell 101. A feed trough 102 is provided on the side of the main body shell 101. A conveyor belt 103 is provided inside the feed trough 102. A plurality of slots 104 are provided on the conveyor belt 103. A locking block 105 is locked inside the slot 104. A reaction cup 106 is provided on the side of the locking block 105.

[0045] The conveyor belt 103 passes through the feed trough 102. The size of the reaction cup 106 is adapted to the size of the feed trough 102. The reaction cup 106 is located inside the feed trough 102. The main body shell 101 has a first slide groove 12 inside, which is located above the feed trough 102.

[0046] Reference Figure 2 The present invention provides a measurement module and a chemiluminescence immunoassay analyzer. The injection assembly 11 includes a motor 111 disposed on the top of the main body shell 101, a lead screw 112 disposed below the motor 111, a slide plate 113 threadedly connected to the lead screw 112, and an injection head 114 disposed at the bottom of the slide plate 113. The lead screw 112 extends through the top of the main body shell 101 to the inside of the first groove 12. The injection head 114 is located on the side of the lead screw 112. The lead screw 112 is slidably connected to the inner wall of the first groove 12. The bottom of the injection head 114 extends through the inner wall of the first groove 12 to the inside of the feed trough 102. The injection head 114 is located directly above the reaction cup 106.

[0047] Reference Figures 2 to 9 This invention provides a measurement module and a chemiluminescence immunoassay analyzer. The measurement component 13 includes a first connecting plate 131 fixedly connected to the side of a slide plate 113. A first rack 132 is fixedly connected to the bottom of the first connecting plate 131. A hollow groove 133 is provided on the first rack 132. A drive gear 134 meshes with the side of the first rack 132. A bevel gear 135 is fixedly connected to the side of the drive gear 134. A transmission gear 136 is provided below the drive gear 134. A second rack 137 meshes with the side of the transmission gear 136. A light shield 138 is fixedly connected to the bottom of the second rack 137. A photon counter 139 is provided inside the light shield 138. The first connecting plate 131 is located on the side of the injection head 114 away from the lead screw 112. The transmission gear 136 is located above the light shield 138. A bevel gear 135 is fixedly connected to the top of the transmission gear 136. The two bevel gears 135 mesh with each other. The first rack 132 is located on the side of the light shield 138 away from the injection head 114. The second rack 137 passes through the hollow groove 133. The second rack 137 and the hollow groove 133 are slidably connected. The first connecting plate 131 and the first sliding groove 12 are slidably connected. The inside of the feed groove 102 is provided with a second sliding groove 14. The light shield 138 and the second sliding groove 14 are slidably connected. The second rack 137 passes through the inner wall of the second sliding groove 14 and extends to the outer side of the main body shell 101. The size of the light shield 138 is adapted to the size of the reaction cup 106. By synchronously linking the light shield 138 with the injection head 114, the light shield adheres to the surface of the reaction cup in time when the substrate is added to form a closed detection space, which isolates external stray light, provides a pure detection environment for the photon counter, avoids signal acquisition errors caused by stray light, and ensures the accurate capture of weak chemiluminescence signals.

[0048] Reference Figures 2 to 11 The present invention provides a measurement module and a chemiluminescence immunoassay analyzer. The adjustment component 20 includes a cylinder 201 disposed inside the main body shell 101. A telescopic rod 202 is disposed inside the cylinder 201. An adjustment shell 203 is fixedly connected to the top of the telescopic rod 202. An annular magnetic block 204 and a main magnetic block 205 are disposed inside the adjustment shell 203. An adjustment groove 206 is opened inside the main body shell 101. The main magnetic block 205 is located at the center of the annular magnetic block 204, the adjusting groove 206 is located below the feeding groove 102, the adjusting groove 206 is connected to the feeding groove 102, and the adjusting housing 203 is slidably connected to the inner wall of the adjusting groove 206. By cooperating with the telescopic rod 202, the gap between the main magnetic block 205 and the bottom outer wall of the reaction cup 106 is precisely controlled at 0.5 to 2 mm, raising the strongest magnetic field focusing surface to the liquid phase region 1 to 3 mm above the bottom of the cup, so that the magnetic beads are suspended inside the liquid, avoiding the problem of light path obstruction and uneven light emission caused by the magnetic beads adhering to the bottom, and ensuring that the light-emitting surface of the magnetic beads is fully exposed.

[0049] Reference Figures 3 to 11 The present invention provides a measurement module and a chemiluminescence immunoassay analyzer. The shielding component 21 includes two third slide grooves 211 opened in the adjustment shell 203. The interior of the third slide groove 211 is slidably connected to a second connecting plate 212. The two second connecting plates 212 are fixedly connected to each other on the side that is close to each other. The interior of the third slide groove 211 is provided with a first elastic element 214 and an air bag 215. The interior of the adjustment shell 203 is provided with a ventilation groove 216. The two sides of the adjustment shell 203 are provided with corrugated pipes 217. Two micro-magnetic shielding sheets 213 are combined to form a shielding plate. The cross-section of the main magnetic block 205 is the same as that of the shielding plate. The shielding plate is located directly above the main magnetic block 205. The second connecting plate 212 is elastically connected to the inner wall of the third sliding groove 211 through the first elastic element 214. The open end of the airbag 215 is fixedly connected to the inner wall of the third sliding groove 211. The airbag 215 is located on the side of the second connecting plate 212 away from the micro-magnetic shielding sheet 213. The side of the airbag 215 close to the second connecting plate 212 is fixedly connected to the second connecting plate 212. The first elastic element 214 is located between the micro-magnetic shielding sheet 213 and the airbag 215. The interior of the corrugated pipe 217 is connected to the interior of the airbag 215 through the ventilation groove 216. The end of the corrugated pipe 217 away from the ventilation groove 216 is fixedly connected to the inner wall of the adjusting groove 206.

[0050] Reference Figures 3 to 8 The present invention provides a measurement module and a chemiluminescence immunoassay analyzer. The transmission component 22 includes a pressure plate 221 fixedly connected to the side of the slide plate 113 away from the first connecting plate 131, and two cavities 224 opened in the main body shell 101. A first trigger plate 222 is provided above the pressure plate 221, and a second trigger plate 223 is provided below the pressure plate 221. A piston rod 225 is slidably connected inside the cavity 224. A second elastic element 226 is provided inside the cavity 224. An air intake groove 227, an air filling groove 228 and a flow diversion groove 229 are opened inside the main body shell 101. The pressure plate 221 is slidably connected to the inner wall of the first slide groove 12. The bottom of the first trigger plate 222 abuts against the top of the pressure plate 221. The piston rod 225 is elastically connected to the inner wall of the cavity 224 through the second elastic member 226. The end of the piston rod 225 away from the second elastic member 226 extends through the inner wall of the cavity 224 into the interior of the first slide groove 12. The sides of the first trigger plate 222 and the second trigger plate 223 that are away from each other are fixedly connected to the piston rod 225. The side of the first trigger plate 222 is provided with an air intake groove 227, and the side of the second trigger plate 223 is provided with an air inflation groove 228. The two cavities 224 are respectively connected to the interior of the diversion groove 229 through the air intake groove 227 and the air inflation groove 228. The air intake groove 227 and the air inflation groove 228 are both connected to the bellows 217 through the diversion groove 229. By setting up a micro magnetic shielding sheet 213, the magnetic field is first weakened during the transport of the reaction cup 106 to prevent the magnetic beads from hitting the wall. During the feeding process, the weakening is released to gather the magnetic beads together. Finally, the magnetic field is weakened again between detections to make the magnetic beads spread out evenly, thus achieving dynamic adjustment. Through the symmetrical gradient weak magnetic field formed by the main magnetic block 205 and the annular magnetic block 204, the magnetic beads are centrally gathered without shifting, sticking to the wall, or accumulating at the edges. Finally, the shielding sheet softens the central peak magnetic field, making the magnetic beads form a uniformly spread state. This avoids the situation where the surface light emission of the magnetic beads is mutually blocked and the signal acquisition error is caused by the magnetic beads being shifted, sticking to the wall, or accumulating at the edges, thus improving the detection stability and accuracy.

[0051] This invention provides a measurement module and a chemiluminescence immunoassay analyzer, the working principle and usage process of which are as follows: First, the test sample, superparamagnetic beads coated with specific antibodies / antigens, and detection reagents labeled with tracer luminescent substances are added to the reaction vessel 106 and placed into the incubation module. After incubation, the immune complex beads are adsorbed onto the reaction vessel wall using an external strong magnetic field, and the waste liquid is discharged. The vessel is then washed multiple times with washing solution to remove unbound free labels and sample impurities to avoid interference with subsequent optical signal detection. Finally, the reaction vessel 106 is conveyed to the inside of the feed tank 102 via conveyor belt 103. Figure 2 As shown, the first trigger plate 222 abuts against the pressure plate 221, the second elastic element 226 above the first trigger plate 222 is in a compressed state, and the two micro magnetic shielding sheets 213 are combined to form a shielding plate, and the first elastic element 214 is in a compressed state, which weakens the magnetic field of the main magnetic block 205 and avoids the situation where the magnetic beads hit the wall due to the strong magnetic field of the main magnetic block 205 during the process of the reaction cup 106 entering the feed tank 102; When the reaction cup 106 is moved directly below the injection head 114, the motor 111 is started to rotate the lead screw 112. The rotation of the lead screw 112 will drive the slide plate 113, which is threaded to it, to move downward. The downward movement of the slide plate 113 will drive the injection head 114 to move into the interior of the reaction cup 106. A chemiluminescent substrate is added into the reaction cup 106 through the injection head 114. The substrate reacts chemically with the tracer luminescent material on the surface of the magnetic bead, and the luminescence process is started. As the sliding plate 113 descends, it simultaneously drives the pressure plate 221 downwards, causing the first trigger plate 222 to lose its support and descend under the elastic force of the second elastic element 226. This generates negative pressure within the cavity 224, which, through the air intake groove 227, diversion groove 229, bellows 217, and ventilation groove 216, creates negative pressure within the airbag 215, causing it to contract. At this time, the second connecting plate 212, under the influence of the negative pressure and the elastic force of the first elastic element 214, drives the micro-magnetic shielding sheet 213 to retract into the third sliding groove 211, releasing the weakening and softening of the magnetic field on the main magnetic block 205, forming a magnetic field with the highest intensity at the center, gradually decreasing radially outwards, and extending to the cup wall and... The symmetrical gradient weak magnetic field at the edge region is the weakest. Under the action of the gradient magnetic field, the superparamagnetic beads automatically gather and are stably constrained towards the center region of the optical path where the magnetic field is strongest. The walls and edge regions of the cup cannot attract the magnetic beads due to the extremely low magnetic field strength. This prevents the magnetic beads from shifting, sticking to the walls, or accumulating at the edges. They are always stably distributed in the center of the detection optical path, avoiding mutual blocking of the light emission from the surface of the magnetic beads and signal acquisition errors caused by the magnetic beads shifting, sticking to the walls, or accumulating at the edges. This improves the stability and accuracy of detection. At the same time, the movement of the superparamagnetic beads in the reaction cup 106 will slowly disturb the substrate in the reaction cup 106, promoting the full chemiluminescence reaction, which can further improve the accuracy of detection. Simultaneously, the cylinder 201 and the telescopic rod 202 cause the adjusting shell 203 to slide within the adjusting groove 206, maintaining a gap of 0.5 to 2 mm between the main magnetic block 205 and the bottom outer wall of the reaction cup 106. Utilizing the spatial distribution law of the magnetic field, the focusing surface with the highest magnetic field strength is moved from the bottom plane of the cup to the liquid phase region 1 to 3 mm above the bottom of the cup. The magnetic bead is constrained in this region to achieve internal suspension and positioning of the liquid, avoiding problems such as light path obstruction, uneven light emission, and poor signal stability caused by the magnetic bead adhering to the bottom. When the pressure plate 221 descends to the position where it abuts the top of the second trigger plate 223, it will press down the second trigger plate 223, causing it to descend and compress the space in the corresponding cavity 224. This allows the gas inside to enter the airbag 215 through the inflation groove 228, the diversion groove 229, the bellows 217, and the ventilation groove 216. Under the action of air pressure, the second connecting plate 212 drives the micro magnetic shielding sheet 213 to move above the main magnetic block 205, weakening and softening the magnetic field of the main magnetic block 205, eliminating the magnetic field interference dead zone between the main magnetic block 205 and the ring magnetic block 204, avoiding excessive focusing and stacking of the magnetic beads, and finally making the magnetic beads form a single layer of uniformly laid out in the central suspension area of ​​the optical path, without agglomeration, retention, or obstruction of the light-emitting surface. Furthermore, during the downward movement of the slide plate 113, the first rack 132 is driven downward synchronously through the first connecting plate 131. The downward movement of the first rack 132 drives the active gear 134, which meshes with it, to rotate. The rotation of the active gear 134 drives the transmission gear 136 to rotate through the two bevel gears 135. The rotation of the transmission gear 136 drives the light shield 138, which meshes with it, to move towards the reaction cup 106 through the second rack 137. When the injection head 114 descends to the injection position, the light shield 138 moves to a position that is in contact with the surface of the reaction cup 106, isolating the external light source. Then, the photon counter 139 located on the side of the reaction cup 106 is activated, and its horizontal light path center is precisely aligned with the magnetic bead levitation layer. The chemiluminescent signal emitted by the tracer material on the surface of the magnetic bead radiates in all directions. Because the magnetic bead is a single layer and has no lateral obstruction, the light signal can be efficiently collected by the photon counter 139 in the light shield 138 without loss or interference, converted into an electrical signal, and transmitted to the data processing unit to complete the measurement.

[0052] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A measurement module and a chemiluminescence immunoassay analyzer, characterized in that: include The measuring component includes an analyzer body (10), an injection assembly (11) for injecting tracer substances is provided inside the analyzer body (10), a first chute (12) and a second chute (14) are provided inside the analyzer body (10), and a measuring assembly (13) for detecting chemiluminescence signals in a reaction cup (106) is provided inside the analyzer body (10). The flattening component includes an adjustment component (20) disposed within the analyzer body (10) for adjusting the magnetic beads in the reaction cup (106), the adjustment component (20) being provided with a shielding component (21) for weakening the magnetic field of the main magnetic block (205), and the analyzer body (10) being provided with a transmission component (22) for adjusting the shielding component (21).

2. The measurement module and chemiluminescence immunoassay analyzer according to claim 1, characterized in that: The analyzer body (10) includes a main body shell (101), a feed trough (102) is provided on the side of the main body shell (101), a conveyor belt (103) is provided inside the feed trough (102), a plurality of slots (104) are provided on the conveyor belt (103), a locking block (105) is locked inside the slot (104), and a reaction cup (106) is provided on the side of the locking block (105). The conveyor belt (103) passes through the feed trough (102). The size of the reaction cup (106) is adapted to the size of the feed trough (102). The reaction cup (106) is located inside the feed trough (102). The main body shell (101) has a first slide groove (12) inside. The first slide groove (12) is located above the feed trough (102).

3. The measurement module and chemiluminescence immunoassay analyzer according to claim 2, characterized in that: The injection assembly (11) includes a motor (111) disposed on the top of the main body shell (101), a lead screw (112) disposed below the motor (111), a slide plate (113) threaded onto the lead screw (112), and an injection head (114) disposed at the bottom of the slide plate (113). The lead screw (112) extends through the top of the main body shell (101) to the interior of the first groove (12). The injection head (114) is located on the side of the lead screw (112). The lead screw (112) is slidably connected to the inner wall of the first groove (12). The bottom of the injection head (114) extends through the inner wall of the first groove (12) to the interior of the feed trough (102). The injection head (114) is located directly above the reaction cup (106).

4. The measurement module and chemiluminescence immunoassay analyzer according to claim 3, characterized in that: The measuring component (13) includes a first connecting plate (131) fixedly connected to the side of the slide plate (113). A first rack (132) is fixedly connected to the bottom of the first connecting plate (131). A hollow groove (133) is provided on the first rack (132). A drive gear (134) meshes with the side of the first rack (132). A bevel gear (135) is fixedly connected to the side of the drive gear (134). A transmission gear (136) is provided below the drive gear (134). A second rack (137) meshes with the side of the transmission gear (136). A light shield (138) is fixedly connected to the bottom of the second rack (137). A photon counter (139) is provided inside the light shield (138).

5. The measurement module and chemiluminescence immunoassay analyzer according to claim 4, characterized in that: The first connecting plate (131) is located on the side of the injection head (114) away from the lead screw (112). The transmission gear (136) is located above the light shield (138). A bevel gear (135) is fixedly connected to the top of the transmission gear (136). The two bevel gears (135) mesh with each other. The first rack (132) is located on the side of the light shield (138) away from the injection head (114). The second rack (137) passes through the hollow groove (133). The second rack (137) is slidably connected to the hollow groove (133). The first connecting plate (131) is slidably connected to the first sliding groove (12). The inside of the feed groove (102) is provided with a second sliding groove (14). The light shield (138) is slidably connected to the second sliding groove (14). The second rack (137) extends through the inner wall of the second sliding groove (14) to the outside of the main body shell (101).

6. The measurement module and chemiluminescence immunoassay analyzer according to claim 2, characterized in that: The adjustment assembly (20) includes a cylinder (201) disposed inside the main body shell (101), a telescopic rod (202) disposed inside the cylinder (201), an adjustment shell (203) fixedly connected to the top of the telescopic rod (202), an annular magnetic block (204) and a main magnetic block (205) disposed inside the adjustment shell (203), and an adjustment groove (206) opened inside the main body shell (101). The main magnetic block (205) is located at the center of the annular magnetic block (204), the adjustment groove (206) is located below the feed groove (102), the adjustment groove (206) is connected to the feed groove (102), and the adjustment shell (203) is slidably connected to the inner wall of the adjustment groove (206).

7. The measurement module and chemiluminescence immunoassay analyzer according to claim 6, characterized in that: The shielding assembly (21) includes two third slide grooves (211) opened in the adjusting shell (203). The interior of the third slide groove (211) is slidably connected to a second connecting plate (212). The two second connecting plates (212) are fixedly connected to each other on the side that is close to each other. The interior of the third slide groove (211) is provided with a first elastic element (214) and an air bag (215). The interior of the adjusting shell (203) is provided with a ventilation groove (216). The two sides of the adjusting shell (203) are provided with corrugated pipes (217).

8. The measurement module and chemiluminescence immunoassay analyzer according to claim 7, characterized in that: Two of the aforementioned micro-magnetic shielding sheets (213) are combined to form a shielding plate. The cross-section of the main magnetic block (205) is the same as that of the shielding plate. The shielding plate is located directly above the main magnetic block (205). The second connecting plate (212) is elastically connected to the inner wall of the third sliding groove (211) through the first elastic element (214). The opening end of the airbag (215) is fixedly connected to the inner wall of the third sliding groove (211). The airbag (215) is located on the second connecting plate (212) away from the micro-magnetic shielding sheet (213). On one side of the shielding sheet (213), the airbag (215) is fixedly connected to the second connecting plate (212) on the side near the second connecting plate (212). The first elastic element (214) is located between the micro magnetic shielding sheet (213) and the airbag (215). The interior of the corrugated tube (217) is connected to the interior of the airbag (215) through the ventilation groove (216). The end of the corrugated tube (217) away from the ventilation groove (216) is fixedly connected to the inner wall of the adjustment groove (206).

9. The measurement module and chemiluminescence immunoassay analyzer according to claim 7, characterized in that: The transmission assembly (22) includes a pressure plate (221) fixedly connected to the side of the slide plate (113) away from the first connecting plate (131), and two cavities (224) opened in the main body shell (101). A first trigger plate (222) is provided above the pressure plate (221), and a second trigger plate (223) is provided below the pressure plate (221). A piston rod (225) is slidably connected inside the cavity (224), and a second elastic element (226) is provided inside the cavity (224). An air intake groove (227), an air filling groove (228), and a flow divider groove (229) are opened inside the main body shell (101).

10. The measurement module and chemiluminescence immunoassay analyzer according to claim 9, characterized in that: The pressure plate (221) is slidably connected to the inner wall of the first slide groove (12), the bottom of the first trigger plate (222) abuts against the top of the pressure plate (221), the piston rod (225) is elastically connected to the inner wall of the cavity (224) through the second elastic member (226), and the end of the piston rod (225) away from the second elastic member (226) extends through the inner wall of the cavity (224) into the interior of the first slide groove (12). The first trigger plate (222) and the second trigger plate (221) are slidably connected to the inner wall of the cavity (12). 23) The two sides that are far apart from each other are fixedly connected to the piston rod (225). The side of the first trigger plate (222) is provided with an air intake groove (227), and the side of the second trigger plate (223) is provided with an air filling groove (228). The two cavities (224) are respectively connected to the interior of the diversion groove (229) through the air intake groove (227) and the air filling groove (228). The air intake groove (227) and the air filling groove (228) are both connected to the bellows (217) through the diversion groove (229).

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