Microorganism-based pesticide-contaminated soil combined remediation structure and method
By constructing a microbial carrier and plant interaction system, combined with irrigation components and ultrasonic catalysts, efficient remediation of soil contaminated with organophosphorus pesticides is achieved, solving the problem of poor remediation effect in existing technologies and significantly improving degradation rate and remediation depth.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SUZHOU GOLD MANTIS GREEN LANDSCAPE LIMITED
- Filing Date
- 2026-04-13
- Publication Date
- 2026-05-15
AI Technical Summary
Existing technologies are ineffective in treating soil contaminated by organophosphorus pesticides. Single remediation methods are not very effective and cannot cope with the uneven distribution and complex occurrence of pollution. They also lack systematicness and long-term effectiveness.
A microbial carrier, a surface activation covering layer, and a plant interaction system are constructed. Combined with irrigation components, an ultrasonic generator, and a catalyst, the system achieves efficient removal and thorough mineralization of pollutants through the synergistic effects of physical adsorption, chemical oxidation, and microbial degradation.
It significantly improves the degradation rate and remediation depth of pesticides, enhances soil structure and microbial diversity, reduces the toxicity of organophosphorus pesticides, and achieves complete remediation of contaminated soil.
Smart Images
Figure CN122033010A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of soil remediation technology, and in particular to a microbial-based structure and method for the combined remediation of pesticide-contaminated soil. Background Technology
[0002] Organophosphorus pesticides are widely used in horticultural planting, but their residues are causing increasingly serious soil pollution problems. Organophosphorus compounds can inhibit the activity of cholinesterase in the soil and disrupt the microbial community. However, existing remediation technologies have the following main shortcomings:
[0003] Chemical oxidation / leaching: Although it is fast-acting, the non-selective oxidation of chemicals can easily damage the physical and chemical properties of the soil, and the remediation is not thorough, which may produce more toxic intermediate products.
[0004] Microbial remediation: This method relies on specific degrading bacteria, but the bacteria are difficult to colonize in the soil, their activity is greatly affected by the environment, the remediation cycle is long, and the remediation effect on high concentrations of pollution is poor.
[0005] Phytoremediation: Limited remediation depth, limited variety of hyperaccumulating plants, and low biomass make it difficult to apply in engineering. These methods are often used alone and are ill-suited to addressing the uneven distribution and complex state of pollution, lacking systematic and long-term remediation structural design. Summary of the Invention
[0006] The purpose of this invention is to provide a microbial-based combined remediation structure and method for pesticide-contaminated soil, in order to address the problem of poor efficacy of single remediation methods for organophosphorus pesticide-contaminated soil.
[0007] To achieve the above objectives, the present invention provides a microbial-based co-remediation structure for pesticide-contaminated soil, comprising:
[0008] A contaminated soil body, wherein a barrier trench is provided around the contaminated soil body, and a barrier wall is provided inside the barrier trench;
[0009] A surface activation coating layer is disposed on the contaminated soil body, and the surface activation coating layer is composed of the contaminated soil body and a remediation agent;
[0010] A microbial carrier is vertically inserted into the contaminated soil body, and several microbial carriers are arranged in a matrix. Microbial agents and catalysts are disposed on the microbial carriers.
[0011] An irrigation assembly is pre-embedded within the contaminated soil. The irrigation assembly is used to inject liquid into the contaminated soil. The irrigation assembly includes several pipes arranged in a grid pattern, and the pipes are provided with permeation holes.
[0012] A reaction tank for storing liquid, and a light source is provided on the reaction tank;
[0013] An ultrasonic generator is disposed on the contaminated soil body, and the ultrasonic generator is used to emit ultrasonic waves.
[0014] As a further description of the above technical solution:
[0015] The microbial carrier is a porous three-dimensional mesh structure made of biodegradable polymer through 3D printing or foaming processes.
[0016] As a further description of the above technical solution:
[0017] The catalyst is a nano-semiconductor titanium dioxide catalyst.
[0018] As a further description of the above technical solution:
[0019] The repair agent includes a chemical oxidant and a biostimulant, wherein the mass ratio of the chemical oxidant to the biostimulant is 25-50%:35-60%.
[0020] As a further description of the above technical solution:
[0021] The chemical oxidizing agent includes persulfate and activator.
[0022] As a further description of the above technical solution:
[0023] The persulfate is sodium persulfate or potassium persulfate, and the activator is ferric ammonium citrate or sodium humate.
[0024] As a further description of the above technical solution:
[0025] The biostimulants include organic carbon sources, nitrogen and phosphorus nutrient sources, and growth-promoting factors for degrading bacteria.
[0026] A microbial-based method for co-remediation of pesticide-contaminated soil includes the following steps:
[0027] S1. Site Investigation and Design: Survey the extent and depth of contamination and select remediation areas;
[0028] S2. Excavate a barrier trench, install a barrier wall in the barrier trench, and install an impermeable membrane between the barrier wall and the contaminated soil.
[0029] S3. Install irrigation components in the contaminated soil.
[0030] S4. The contaminated soil is repeatedly soaked with water using the irrigation assembly, and the soaking water is discharged into the reaction tank. The light source is turned on to irradiate the soaking water.
[0031] S5. Install microbial carriers and lay a surface activation covering layer on the contaminated soil.
[0032] S6. Every so often, the contaminated soil is soaked with water and the ultrasonic device is turned on. Then the soaking water is discharged into the reaction tank and the light source is turned on to irradiate the soaking water.
[0033] S7. Monitor pesticide residues and microbial activity in contaminated soil until the remediation target is achieved.
[0034] As a further description of the above technical solution:
[0035] In step S5, plants are planted in the surface activation cover layer.
[0036] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:
[0037] 1. In this invention, by constructing an interaction system of microbial carrier, surface activation covering layer and plant, efficient colonization and activity maintenance of microorganisms and maximum contact between pollutants, microorganisms and plants are achieved, thereby significantly improving the pesticide degradation rate and remediation depth.
[0038] 2. In this invention, by setting up an irrigation component, water and nutrients can be irrigated onto the contaminated soil, providing a good growth environment for microorganisms and plants. By soaking the contaminated soil, the pesticides inside the soil can be dissolved in the water. The water is then discharged into a reaction tank, and then irradiated by a light source. The light energy directly acts on the phosphoester bonds of the organophosphorus pesticides, causing them to break and generate free electrons, thereby hydrolyzing the organophosphorus pesticides and reducing their toxicity. In a watery or humid environment, as long as light is present, the phosphoester bonds are easily activated to undergo hydrolysis. The hydrolysis of one phosphoester bond of parathion can reduce its toxicity by 100 times, thereby achieving the purpose of remediating contaminated soil.
[0039] 3. In this invention, by using an ultrasonic generator, a catalyst, and persulfate, after soaking the contaminated soil with water, the soaking water contains organophosphorus pesticides, titanium dioxide catalyst, and persulfate.
[0040] After the ultrasonic generator is activated, the cavitation effect generated by the ultrasound in the liquid releases enormous energy, effectively stimulating the catalytic activity of nano-titanium dioxide and generating highly reactive hydroxyl radicals on its surface. Simultaneously, due to the strong adsorption capacity of the nano-titanium dioxide, a large number of organophosphorus pesticide molecules are enriched on the catalyst surface. Under the action of the active hydroxyl radicals generated on the surface, the adsorbed pesticide pollutants undergo an oxidation reaction, gradually mineralizing into carbon dioxide, water, and simple inorganic ions such as nitrate, sulfate, phosphate, and halide ions. Subsequently, under the action of the microjets and shock waves generated by ultrasonic cavitation, these generated inorganic ions desorb from the catalyst surface and diffuse into the liquid phase, re-exposing the catalyst's active sites. New organophosphorus pesticide molecules then begin the next round of adsorption-catalytic oxidation-desorption dynamic cycle, thereby achieving continuous and efficient removal of pollutants and deep purification of wastewater. Attached Figure Description
[0041] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.
[0042] Figure 1 This is a schematic diagram of a microbial-based co-remediation structure for pesticide-contaminated soil.
[0043] Figure 2 This is a flowchart of a microbial-based method for the joint remediation of pesticide-contaminated soil.
[0044] Legend:
[0045] 1. Contaminated soil body; 2. Barrier wall; 3. Surface activation cover layer; 4. Microbial carrier; 5. Irrigation component; 6. Reaction tank; 7. Ultrasonic generator; 8. Light source. Detailed Implementation
[0046] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. The components of the embodiments of the present invention described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.
[0047] Therefore, the following detailed description of the embodiments of the invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments of the invention. All other embodiments obtained by those skilled in the art based on the embodiments of the invention without inventive effort are within the scope of protection of the invention.
[0048] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.
[0049] In the description of the embodiments of the present invention, it should be noted that the terms "upper" and "inner" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship in which the product of the invention is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting the present invention.
[0050] In the description of this invention, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.
[0051] Example 1:
[0052] Please see Figure 1-2 This invention provides a microbial-based co-remediation structure for pesticide-contaminated soil, comprising:
[0053] A contaminated soil body 1 is surrounded by a barrier trench, and a barrier wall 2 is installed inside the barrier trench.
[0054] Surface activation cover layer 3, the surface activation cover layer 3 is disposed on the contaminated soil body 1, the surface activation cover layer 3 is composed of contaminated soil body 1 and remediation agent;
[0055] Microbial carrier 4 is vertically inserted into the contaminated soil body 1, and several microbial carriers 4 are arranged in a matrix. Microbial inoculants and catalysts are provided on the microbial carriers 4.
[0056] An irrigation assembly 5 is pre-embedded within the contaminated soil body 1. The irrigation assembly is used to inject liquid into the contaminated soil body 1. The irrigation assembly includes several pipes arranged in a grid pattern, and the pipes are provided with permeation holes.
[0057] Reaction tank 6, the reaction tank is used to store liquid, and a light source 8 is provided on the reaction tank;
[0058] An ultrasonic generator 7 is disposed on the contaminated soil body 1, and the ultrasonic generator 7 is used to emit ultrasonic waves.
[0059] Specifically, the catalyst is a nano-semiconductor titanium dioxide catalyst.
[0060] A microbial-based method for co-remediation of pesticide-contaminated soil includes the following steps:
[0061] S1. Site Investigation and Design: Survey the extent and depth of contamination and select remediation areas;
[0062] S2. Excavate a barrier trench, install a barrier wall in the barrier trench, and install an impermeable membrane between the barrier wall and the contaminated soil.
[0063] S3. Install irrigation components in the contaminated soil.
[0064] S4. The contaminated soil is repeatedly soaked with water using the irrigation assembly, and the soaking water is discharged into the reaction tank. The light source is turned on to irradiate the soaking water.
[0065] S5. Install microbial carriers and lay a surface activation covering layer on the contaminated soil.
[0066] S6. Every so often, the contaminated soil is soaked with water and the ultrasonic device is turned on. Then the soaking water is discharged into the reaction tank and the light source is turned on to irradiate the soaking water.
[0067] S7. Monitor pesticide residues and microbial activity in contaminated soil until the remediation target is achieved.
[0068] In step S5, plants are planted in the surface activation cover layer.
[0069] This method employs a multi-mechanism synergistic effect: physical adsorption, chemical oxidation, microbial degradation, and phytoabsorption and transformation occur in an orderly manner within the same space, achieving efficient removal and thorough mineralization of pollutants. While remediating pollution, it significantly improves soil aggregate structure, organic matter content, and microbial diversity, restoring land productivity.
[0070] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:
[0071] 1. In this invention, by constructing an interaction system of microbial carrier, surface activation covering layer and plant, efficient colonization and activity maintenance of microorganisms and maximum contact between pollutants, microorganisms and plants are achieved, thereby significantly improving the pesticide degradation rate and remediation depth.
[0072] 2. In this invention, by setting up an irrigation component, water and nutrients can be irrigated onto the contaminated soil, providing a good growth environment for microorganisms and plants. By soaking the contaminated soil, the pesticides inside the soil can be dissolved in the water. The water is then discharged into a reaction tank, and then irradiated by a light source. The light energy directly acts on the phosphoester bonds of the organophosphorus pesticides, causing them to break and generate free electrons, thereby hydrolyzing the organophosphorus pesticides and reducing their toxicity. In a watery or humid environment, as long as light is present, the phosphoester bonds are easily activated to undergo hydrolysis. The hydrolysis of one phosphoester bond of parathion can reduce its toxicity by 100 times, thereby achieving the purpose of remediating contaminated soil.
[0073] 3. In this invention, by using an ultrasonic generator, a catalyst, and persulfate, after soaking the contaminated soil with water, the soaking water contains organophosphorus pesticides, titanium dioxide catalyst, and persulfate.
[0074] After the ultrasonic generator is activated, the cavitation effect generated by the ultrasound in the liquid releases enormous energy, effectively stimulating the catalytic activity of nano-titanium dioxide and generating highly reactive hydroxyl radicals on its surface. Simultaneously, due to the strong adsorption capacity of the nano-titanium dioxide, a large number of organophosphorus pesticide molecules are enriched on the catalyst surface. Under the action of the active hydroxyl radicals generated on the surface, the adsorbed pesticide pollutants undergo an oxidation reaction, gradually mineralizing into carbon dioxide, water, and simple inorganic ions such as nitrate, sulfate, phosphate, and halide ions. Subsequently, under the action of the microjets and shock waves generated by ultrasonic cavitation, these generated inorganic ions desorb from the catalyst surface and diffuse into the liquid phase, re-exposing the catalyst's active sites. New organophosphorus pesticide molecules then begin the next round of adsorption-catalytic oxidation-desorption dynamic cycle, thereby achieving continuous and efficient removal of pollutants and deep purification of wastewater.
[0075] Example 2:
[0076] See Figure 1-2The figure shows a structure and method for co-remediation of pesticide-contaminated soil based on microorganisms, provided in Embodiment 2 of the present invention. This embodiment further improves upon the above embodiments by making the following technical solutions: The microbial carrier 4 is a porous three-dimensional mesh structure made of a biodegradable polymer through 3D printing or foaming processes. The polymer is lactic acid-glycolic acid copolymer (PLGA) or starch-based material, with a mesh unit size of 1-5 cm and a porosity >70%. The microbial agent is encapsulated in sodium alginate and biochar microcapsules to achieve slow release. The negative microbial agent is a composite agent targeting the target pesticides, including Pseudomonas, Alcaligenes, and white-rot fungi. The carrier surface is coated with nutrient slow-release particles containing nitrogen, phosphorus, trace elements, and acyl homoserine lactone, used to induce microbial quorum sensing and biofilm formation.
[0077] Example 3:
[0078] See Figure 1-2 The figure illustrates a microbial-based structure and method for the joint remediation of pesticide-contaminated soil provided in Embodiment 3 of the present invention. This embodiment further improves upon the previous embodiments by incorporating the following technical solutions: The remediation agent comprises a chemical oxidant and a biostimulant, with a mass ratio of 25-50%:35-60%. The chemical oxidant comprises persulfate and an activator. The persulfate is sodium persulfate or potassium persulfate, and the activator is ferric ammonium citrate or sodium humate. The persulfate is activated by the activator, generating free radicals on the surface, rapidly breaking the PO or PS bonds of organophosphorus molecules, reducing their toxicity and stability. The small molecule products generated by chemical oxidation and the nutrients released by the compound agent jointly stimulate the proliferation of native soil microorganisms and exogenous inoculants, thoroughly mineralizing intermediate products into CO2, H2O, phosphate, and inorganic salts. Simultaneously, the use of ultrasound can improve decomposition efficiency.
[0079] Example 4:
[0080] See Figure 1-2 The figure illustrates a structure and method for the combined remediation of pesticide-contaminated soil based on microorganisms, as provided in Embodiment 4 of the present invention. This embodiment further improves upon the previous embodiments by incorporating the following technical solutions: The biostimulant comprises an organic carbon source, a nitrogen and phosphorus nutrient source, and a growth-promoting factor for degrading bacteria. The organic carbon source is sugarcane bagasse or corn stalk powder, providing a slow-release carbon source; the nitrogen and phosphorus nutrient source is diammonium hydrogen phosphate; and the growth-promoting factor for degrading bacteria is yeast extract powder, providing balanced nutrition to exogenous degrading microorganisms, stimulating their metabolic activity, and accelerating the further mineralization of chemical oxidation products.
[0081] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.
Claims
1. A microbial-based co-remediation structure for pesticide-contaminated soil, characterized in that, include: A contaminated soil body, wherein a barrier trench is provided around the contaminated soil body, and a barrier wall is provided inside the barrier trench; A surface activation coating layer is disposed on the contaminated soil body, and the surface activation coating layer is composed of the contaminated soil body and a remediation agent; A microbial carrier is vertically inserted into the contaminated soil body, and several microbial carriers are arranged in a matrix. Microbial agents and catalysts are disposed on the microbial carriers. An irrigation assembly is pre-embedded within the contaminated soil. The irrigation assembly is used to inject liquid into the contaminated soil. The irrigation assembly includes several pipes arranged in a grid pattern, and the pipes are provided with permeation holes. A reaction tank for storing liquid, and a light source is provided on the reaction tank; An ultrasonic generator is disposed on the contaminated soil body, and the ultrasonic generator is used to emit ultrasonic waves.
2. The microbial-based co-remediation structure for pesticide-contaminated soil according to claim 1, characterized in that, The microbial carrier is a porous three-dimensional mesh structure made of biodegradable polymer through 3D printing or foaming processes.
3. The microbial-based co-remediation structure for pesticide-contaminated soil according to claim 1, characterized in that, The catalyst is a nano-semiconductor titanium dioxide catalyst.
4. The microbial-based co-remediation structure for pesticide-contaminated soil according to claim 1, characterized in that, The repair agent includes a chemical oxidant and a biostimulant, wherein the mass ratio of the chemical oxidant to the biostimulant is 25-50%:35-60%.
5. The microbial-based co-remediation structure for pesticide-contaminated soil according to claim 4, characterized in that, The chemical oxidizing agent includes persulfate and activator.
6. The microbial-based co-remediation structure for pesticide-contaminated soil according to claim 5, characterized in that, The persulfate is sodium persulfate or potassium persulfate, and the activator is ferric ammonium citrate or sodium humate.
7. The microbial-based co-remediation structure for pesticide-contaminated soil according to claim 1, characterized in that, The biostimulants include organic carbon sources, nitrogen and phosphorus nutrient sources, and growth-promoting factors for degrading bacteria.
8. A microbial-based method for the joint remediation of pesticide-contaminated soil, characterized in that, Includes the following steps: S1. Site Investigation and Design: Survey the extent and depth of contamination and select remediation areas; S2. Excavate a barrier trench, install a barrier wall in the barrier trench, and install an impermeable membrane between the barrier wall and the contaminated soil. S3. Install irrigation components in the contaminated soil. S4. The contaminated soil is repeatedly soaked with water using the irrigation assembly, and the soaking water is discharged into the reaction tank. The light source is turned on to irradiate the soaking water. S5. Install microbial carriers and lay a surface activation covering layer on the contaminated soil. S6. Every so often, the contaminated soil is soaked with water and the ultrasonic device is turned on. Then the soaking water is discharged into the reaction tank and the light source is turned on to irradiate the soaking water. S7. Monitor pesticide residues and microbial activity in contaminated soil until the remediation target is achieved.
9. A method for co-remediation of pesticide-contaminated soil based on microorganisms according to claim 8, characterized in that, In step S5, plants are planted in the surface activation cover layer.