Bacterial biofilm exchange device on culture solution plate
By designing culture medium conditioning equipment and culture dish conditioning equipment, the problem of separating culture medium from bacteria on culture medium plates was solved, realizing efficient exchange and detection of biofilms, and facilitating operation.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SICHUAN ACADEMY OF MEDICAL SCI SICHUAN PROVINCIAL PEOPLES HOSPITAL
- Filing Date
- 2023-08-07
- Publication Date
- 2026-05-15
AI Technical Summary
Existing bacterial biofilm exchange devices on culture plates cannot effectively separate the culture medium from the bacteria, affecting the subsequent biofilm exchange process.
A device was designed that includes a culture medium adjustment device and a culture dish adjustment device. The separation of culture medium and bacterial organisms is achieved through a combination of connecting frames, supporting plates, and adjusting frames. Bacterial growth is promoted by dripping the culture medium into the culture dish through a guide valve. The height of the culture dish is adjusted by a motor-driven screw system for easy detection.
This method achieves effective separation of culture medium and bacterial organisms, simplifies the biofilm exchange process, and improves operational efficiency and biofilm retention rate.
Smart Images
Figure CN122038092A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of bacterial culture technology, specifically a device for exchanging bacterial biofilm on a culture plate. Background Technology
[0002] Biofilms, also known as biological membranes, are organized communities of bacteria attached to the surface of living or non-living objects and encapsulated by extracellular macromolecules. Biofilm bacteria exhibit strong resistance to antibiotics and host immune defense mechanisms. Biofilms contain various major biological macromolecules such as proteins, polysaccharides, DNA, RNA, peptidoglycans, lipids, and phospholipids. The formation of multicellular biofilm structures is a dynamic process, including stages such as bacterial initiation of adhesion, biofilm development, and maturation and diffusion.
[0003] As the closest prior art, Chinese Patent Publication No. CN218879896U relates to the field of medical experimental equipment technology and discloses a bacterial biofilm replacement device on a culture medium plate. By attaching the bacterial biofilm to the inner wall of the bacterial biofilm culture cup, the device effectively protects the bacterial biofilm. During cleaning, the bacterial biofilm culture medium plate placed in the culture medium plate can be lifted directly and replaced with a new culture medium plate, which greatly shortens the operation time, reduces the operation difficulty, and improves the biofilm retention rate. It is suitable for high-throughput biofilm determination.
[0004] However, existing bacterial biofilm exchange devices on culture plates and the aforementioned cases mostly fail to separate the culture medium from the bacteria during use, thus affecting subsequent bacterial biofilm exchange. Therefore, improvements are needed. Summary of the Invention
[0005] To address the problems in the prior art, the present invention provides a device for exchanging bacterial biofilm on a culture plate.
[0006] The technical solution adopted by the present invention to solve its technical problem is: a bacterial biofilm exchange device on a culture medium plate, including a culture medium adjustment device and a culture dish adjustment device, wherein the lower end of the culture medium adjustment device is limited and connected to the culture dish adjustment device;
[0007] The culture medium adjustment device adopts an adjustable structure design, which can separate the culture medium from the bacterial organisms;
[0008] The culture dish conditioning device can be used for the culture of bacterial organisms and can also quickly replace bacterial biofilms.
[0009] The culture medium adjustment device includes a combined frame, a supporting plate, a culture medium protective shell, an internal conduit, a drain valve, and an adjustment frame. The upper end of the adjustment frame is fixedly connected to the combined frame, and the side end of the combined frame is fixedly connected to the supporting plate. The center of the supporting plate is provided with a culture medium protective shell, and the culture medium protective shells are arranged in a rectangular pattern. The lower end of the culture medium protective shell is connected to an internal conduit, and the lower end of the internal conduit is connected to a drain valve.
[0010] Specifically, the culture dish adjustment device includes a positioning protection frame, a rotating coupling, a culture dish, and a pusher. The four corners of the side end of the positioning protection frame are rotatably connected to the rotating coupling, and the center of the positioning protection frame is limited to the culture dish. The lower end of the culture dish is limited to the pusher. Through the structural arrangement of the combined frame, supporting plate, and adjusting frame, the positions of the culture medium protective shell, the internal conduit, and the drain valve can be adjusted. The culture medium protective shell, the internal conduit, and the drain valve are connected as a whole, allowing the culture medium to be placed. The culture medium can be dripped into the interior of the culture dish through the drain valve to help bacterial growth, achieving the purpose of separating the culture medium from the bacterial organisms, facilitating subsequent biofilm exchange. The adjusting frame can be rotated and adjusted on the culture dish adjustment device, thereby changing the position of the upper structure.
[0011] Specifically, the guiding unit includes a protective partition, a limiting tray, a first motor, a limiting guide block, and a first lead screw. A limiting guide block is fixedly connected to the side end of the protective partition, and a limiting tray is fixedly connected to the center of the limiting guide block. A first motor is mounted on the top of the limiting guide block, and a first lead screw is driven and connected to the lower end of the first motor. A combined connecting block is threadedly connected to the lower end of the first lead screw, and a pushing guide plate is fixedly connected to the rear end of the combined connecting block. A sliding rod is provided on the side of the limiting guide block opposite to the first lead screw, and the side end of the combined connecting block is slidably connected to the sliding rod. Through the structural settings of the culture dish adjustment device, bacteria are cultured inside the culture dish. The positioning protective frame and limiting tray can limit the position of the culture dish. Simultaneously, when it needs to be removed for testing, the first motor can drive the first lead screw to rotate, changing the position of the combined connecting block and the pushing guide plate, so that the pushing guide plate lifts the culture dish, adjusting the height of the culture dish to facilitate the detection and processing of bacteria inside the culture dish.
[0012] Specifically, the bottom of the protective partition is fixedly connected to a support base plate, and the support base plate is fixedly connected to the bottom of the limiting guide block.
[0013] Specifically, the positioning protection frame and the limiting tray are arranged in parallel, and both the positioning protection frame and the limiting tray are used for limiting the connection of the petri dish.
[0014] Specifically, the positioning protection frame is connected to the adjustment frame via a rotating coupling, and the combined frame, support plate, culture medium protective shell, built-in conduit, and guide valve are rotated and adjusted via the adjustment frame.
[0015] Specifically, the combined connecting blocks can support the culture dish and change its height position by pushing the guide plate.
[0016] Specifically, a rotating gear disk is fixedly connected to the front end of the rotating shaft, a gear guide plate is meshed with the side end of the rotating gear disk, a mating guide block is fixedly connected to the side end of the gear guide plate, a second lead screw is threaded to the center of the mating guide block, a second motor is installed at the bottom of the second lead screw, and a positioning protection seat is fixedly installed on the side end of the second motor.
[0017] The beneficial effects of this invention are:
[0018] First, this invention, through the structural arrangement of a combined connecting frame, supporting connecting plate, and adjusting connecting frame, allows for the adjustment of the positions of the culture medium protective shell, internal conduit, and drain valve. The culture medium protective shell, internal conduit, and drain valve are interconnected, enabling the placement of the culture medium. Furthermore, the culture medium can be dripped into the interior of the culture dish through the drain valve to aid bacterial growth, achieving the purpose of separating the culture medium from the bacterial organisms. This facilitates subsequent biofilm exchange. The adjusting connecting frame can be rotated and adjusted on the culture dish adjustment device, thereby changing the position of the upper structure.
[0019] Second, this invention uses the structural design of a petri dish adjustment device to cultivate bacteria. The bacteria are cultured inside the petri dish, and the positioning and protective frame and limiting plate can limit the position of the petri dish. At the same time, when it is necessary to remove it for testing, the first motor can drive the first lead screw to rotate, change the position of the combined connecting block and the push guide plate, so that the push guide plate lifts the petri dish and adjusts the height of the petri dish, which facilitates the detection and processing of bacteria inside the petri dish. Attached Figure Description
[0020] The present invention will be further described below with reference to the accompanying drawings and embodiments.
[0021] Figure 1 This is a three-dimensional structural diagram of the main body from a frontal perspective in this invention;
[0022] Figure 2 This is a side view three-dimensional structural diagram of the main body in this invention;
[0023] Figure 3 This is a split view of the main body of the invention;
[0024] Figure 4 This is an exploded view of the petri dish conditioning device in this invention;
[0025] Figure 5 This is a split view of the derivation section in this invention;
[0026] Figure 6 This is a frontal perspective three-dimensional structural diagram of the second embodiment of the main body of the present invention.
[0027] In the diagram: 1-Culture medium adjustment device, 2-Culture dish adjustment device, 3-Combined frame, 4-Supporting plate, 5-Culture medium protective shell, 6-Built-in tubing, 7-Guide valve, 8-Adjusting frame, 9-Positioning protection frame, 10-Rotating shaft, 11-Culture dish, 12-Pushing part, 13-Protective partition, 14-Restricting support plate, 15-First motor, 16-Limiting guide block, 17-First lead screw, 18-Combined block, 19-Pushing guide plate, 20-Support base plate, 21-Rotating gear plate, 22-Second lead screw, 23-Gear guide plate, 24-Matching guide block, 25-Positioning protection seat, 26-Second motor. Detailed Implementation
[0028] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present application, and not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative effort should fall within the scope of protection of the present application.
[0029] It should be noted that the terms "first," "second," etc., in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate for the embodiments of this application described herein. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion; for example, a process, method, system, product, or apparatus that comprises a series of steps or units is not necessarily limited to those steps or units explicitly listed, but may include other steps or units not explicitly listed or inherent to such processes, methods, products, or apparatus.
[0030] The invention will be further described below with reference to the accompanying drawings.
[0031] Example 1
[0032] like Figure 1-5 As shown, a bacterial biofilm exchange device on a culture medium plate according to the present invention includes a culture medium adjustment device 1 and a culture dish adjustment device 2, wherein the lower end of the culture medium adjustment device 1 is limited and connected to the culture dish adjustment device 2.
[0033] The culture medium conditioning device 1 adopts an adjustable structure design, which can separate the culture medium from the bacterial organisms;
[0034] The culture dish conditioning device 2 can be used for the culture of bacterial organisms and can also quickly replace bacterial biofilms.
[0035] like Figure 3 As shown, the culture medium adjustment device 1 includes a combined frame 3, a supporting plate 4, a culture medium protective shell 5, an internal conduit 6, a drain valve 7, and an adjustment frame 8. The upper end of the adjustment frame 8 is fixedly connected to the combined frame 3, and the side end of the combined frame 3 is fixedly connected to the supporting plate 4. The center of the supporting plate 4 is provided with the culture medium protective shell 5, and the culture medium protective shell 5 is arranged in a rectangular pattern. The lower end of the culture medium protective shell 5 is connected to the internal conduit 6, and the lower end of the internal conduit 6 is connected to the drain valve 7. The combined frame 3, the supporting plate 4, and the adjustment frame 8 are fixed together, which can drive the culture medium protective shell 5, the internal conduit 6, and the drain valve 7 to move and adjust. At the same time, the culture medium inside the culture medium protective shell 5 and the internal conduit 6 can be conducted through the drain valve 7, which facilitates the bacterial culture process.
[0036] like Figure 4 As shown, the petri dish adjustment device 2 includes a positioning protection frame 9, a rotating coupling 10, a petri dish 11, and a pushing part 12. The four corners of the side end of the positioning protection frame 9 are rotatably connected to the rotating coupling 10. The center of the positioning protection frame 9 is limited and connected to the petri dish 11. The lower end of the petri dish 11 is limited and connected to the pushing part 12.
[0037] like Figure 5 As shown, the push-guide section 12 includes a protective partition 13, a limiting support plate 14, a first motor 15, a limiting guide block 16, and a first lead screw 17. The limiting guide block 16 is fixedly connected to the side end of the protective partition 13. The limiting support plate 14 is fixedly connected to the center of the limiting guide block 16. The first motor 15 is mounted on the top of the limiting guide block 16. The first lead screw 17 is driven and connected to the lower end of the first motor 15. A combined connecting block 18 is threadedly connected to the lower end of the first lead screw 17. A push guide plate 19 is fixedly connected to the rear end of the combined connecting block 18. A sliding rod is provided on the side of the limiting guide block 16 opposite to the first lead screw 17. The side end of block 18 is slidably connected to the slide rod. Rotating the adjusting frame 8 and rotating the connecting shaft 10 changes the position of the combined frame 3 and the supporting plate 4, so that the culture medium protective shell 5, the internal conduit 6, the guide valve 7 and the culture dish 11 are separated. Then, the first motor 15 is driven to work. The first motor 15 drives the first lead screw 17 to rotate, so that the combined block 18 can slide under the limit of the slide rod. The movement of the combined block 18 drives the push guide plate 19 to follow the movement. The push guide plate 19 can drag the culture dish 11 to change its position, making it convenient for the user to pick up the culture dish 11.
[0038] The bottom of the protective partition 13 is fixedly connected to the support base plate 20, and the support base plate 20 is fixedly connected to the bottom of the limiting guide block 16.
[0039] The positioning protection frame 9 and the limiting tray 14 are arranged in parallel. Both the positioning protection frame 9 and the limiting tray 14 are used to limit the connection of the petri dish 11, which facilitates the limitation of the position of the petri dish 11. Due to the narrow opening design, the petri dish 11 cannot fall.
[0040] The positioning protection frame 9 is connected to the adjusting frame 8 via the rotating coupling 10. The combined frame 3, the supporting plate 4, the culture medium protective shell 5, the built-in conduit 6, and the guide valve 7 are rotated and adjusted via the adjusting frame 8.
[0041] The combined connecting block 18 can support the culture dish 11 by pushing the guide plate 19, changing the height position of the culture dish 11, thereby facilitating the inspection of the culture dish 11.
[0042] The working principle of Example 1 is as follows: When in use, the culture medium conditioning device 1 and the culture dish conditioning device 2 are combined as a whole to facilitate the culture work;
[0043] Then, the petri dish 11 is placed inside the positioning protection frame 9 and the limiting tray 14, and the positioning protection frame 9 and the limiting tray 14 are used to limit the installation of the petri dish 11.
[0044] Afterwards, the user places the culture medium protective shell 5, the internal conduit 6, and the drain valve 7 on the upper end of the support plate 4, and at the same time puts the culture medium into the culture medium protective shell 5, the internal conduit 6, and the drain valve 7. The culture medium can reach the inner end of the culture dish 11 through the drain valve 7 to carry out bacterial culture.
[0045] Then, when the user needs to observe, they can rotate the adjusting frame 8 and the rotating shaft 10 to change the position of the combined frame 3 and the supporting plate 4, so that the culture medium protective shell 5, the internal conduit 6, the guide valve 7 and the culture dish 11 are separated. Then, the first motor 15 is driven to work. The first motor 15 drives the first lead screw 17 to rotate, so that the combined block 18 can slide under the limit of the slide rod. The movement of the combined block 18 drives the push guide plate 19 to follow the movement. The push guide plate 19 can drag the culture dish 11 to change its position, making it convenient for the user to pick up the culture dish 11.
[0046] Finally, the protective partition 13, the limiting guide block 16, and the support base plate 20 are fixed to facilitate the overall structure's load-bearing installation. The limiting support plate 14 is fixed inside the protective partition 13, the limiting guide block 16, and the support base plate 20, and the support connection work of the positioning protection frame 9 is carried out at the same time to complete the work.
[0047] Example 2
[0048] Based on Example 1, such as Figure 6 As shown, a rotating gear disk 21 is fixedly connected to the front end of the rotating shaft 10. A gear guide plate 23 is meshed with the side end of the rotating gear disk 21. A mating guide block 24 is fixedly connected to the side end of the gear guide plate 23. A second lead screw 22 is threadedly connected to the center of the mating guide block 24. A second motor 26 is installed at the bottom of the second lead screw 22. A positioning protection seat 25 is fixedly installed on the side end of the second motor 26.
[0049] When implementing this embodiment, when testing is required, the user can drive the second motor 26 to work. The second motor 26 drives the second lead screw 22 to run, so that the upper connected mating guide block 24 moves under the limit of the positioning protection seat 25, thereby driving the toothed guide plate 23 to change position. When the toothed guide plate 23 moves, it can drive the rotating toothed disk 21 to rotate. Then the rotating toothed disk 21 drives the fixed rotating shaft 10 to rotate, thereby changing the position of the supporting connecting plate 4, the combined connecting frame 3, and the adjusting connecting frame 8.
[0050] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.
Claims
1. A device for exchanging bacterial biofilm on a culture plate, characterized in that: It includes a culture medium conditioning device (1) and a culture dish conditioning device (2), wherein the lower end of the culture medium conditioning device (1) is limited and connected to the culture dish conditioning device (2); The culture medium adjustment device (1) adopts an adjustable structure design, which can separate the culture medium from the bacterial organisms; The culture dish conditioning device (2) can perform bacterial culture and can also quickly replace bacterial biofilm. The culture medium adjustment device (1) includes a combined frame (3), a supporting plate (4), a culture medium protective shell (5), an internal conduit (6), a drain valve (7), and an adjustment frame (8). The upper end of the adjustment frame (8) is fixedly connected to the combined frame (3), and the side end of the combined frame (3) is fixedly connected to the supporting plate (4). The center of the supporting plate (4) is provided with a culture medium protective shell (5), and the culture medium protective shells (5) are arranged in a rectangular pattern. The lower end of the culture medium protective shell (5) is connected to the internal conduit (6), and the lower end of the internal conduit (6) is connected to the drain valve (7).
2. The bacterial biofilm exchange device on a culture plate according to claim 1, characterized in that: The culture dish adjustment device (2) includes a positioning protection frame (9), a rotating coupling (10), a culture dish (11), and a pushing part (12). The four corners of the side end of the positioning protection frame (9) are rotatably connected to the rotating coupling (10). The center of the positioning protection frame (9) is limited and connected to the culture dish (11). The lower end of the culture dish (11) is limited and connected to the pushing part (12).
3. The bacterial biofilm exchange device on a culture plate according to claim 2, characterized in that: The push-guide section (12) includes a protective partition (13), a limiting plate (14), a first motor (15), a limiting guide block (16), and a first lead screw (17). The limiting guide block (16) is fixedly connected to the side end of the protective partition (13). The limiting plate (14) is fixedly connected to the center of the limiting guide block (16). The first motor (15) is installed on the top of the limiting guide block (16). The first lead screw (17) is driven and connected to the lower end of the first motor (15). The combined connecting block (17) is threadedly connected to the lower end of the first lead screw (17). The push guide plate (19) is fixedly connected to the rear end of the combined connecting block (18). A sliding rod is provided on the side of the limiting guide block (16) away from the first lead screw (17). The side end of the combined connecting block (18) is slidably connected to the sliding rod.
4. The bacterial biofilm exchange device on a culture plate according to claim 3, characterized in that: The bottom of the protective partition (13) is fixedly connected to a support base plate (20), and the support base plate (20) is fixedly connected to the bottom of the limiting guide block (16).
5. The bacterial biofilm exchange device on a culture plate according to claim 4, characterized in that: The positioning protection frame (9) and the limiting tray (14) are arranged in parallel, and both the positioning protection frame (9) and the limiting tray (14) are used for the limiting connection of the petri dish (11).
6. The bacterial biofilm exchange device on a culture plate according to claim 5, characterized in that: The positioning protection frame (9) is connected to the adjustment frame (8) via a rotating shaft (10). The combined frame (3), the supporting plate (4), the culture medium protective shell (5), the built-in conduit (6), and the guide valve (7) are rotated and adjusted via the adjustment frame (8).
7. The bacterial biofilm exchange device on a culture plate according to claim 6, characterized in that: The combined connecting block (18) can support the culture dish (11) by pushing the guide plate (19) and change the height position of the culture dish (11).
8. The bacterial biofilm exchange device on a culture plate according to claim 7, characterized in that: The front end of the rotating shaft (10) is fixedly connected to a rotating gear disk (21), and the side end of the rotating gear disk (21) is meshed with a gear guide plate (23). The side end of the gear guide plate (23) is fixedly connected to a mating guide block (24). The center thread of the mating guide block (24) is connected to a second lead screw (22). The bottom of the second lead screw (22) is equipped with a second motor (26), and the side end of the second motor (26) is fixedly installed with a positioning protection seat (25).