A high performance liquid chromatograph ultraviolet detector

Abstract

The application discloses a high-efficiency liquid chromatograph ultraviolet detector, which comprises a main detector shell, a liquid inlet assembly, a separation and detection assembly and a recognition assembly.

Description

Technical Field

[0001] This invention relates to the field of optical detection technology, and more particularly to a high-performance liquid chromatograph ultraviolet detector. Background Technology

[0002] High-performance liquid chromatography (HPLC) ultraviolet (UV) detectors are core detection equipment in fields such as pharmaceutical analysis and environmental monitoring, and their performance directly determines the accuracy and reliability of qualitative and quantitative analysis. Existing UV detectors generally adopt a three-stage structure of "mobile phase delivery - chromatographic separation - UV detection." A stable supply of the mobile phase is achieved through a reservoir and a pump; sample components are separated using a chromatographic column; and the UV light source, grating, and flow cell work together to achieve optical signal detection. Some high-end devices integrate a simple spectral data processing module to meet basic detection needs.

[0003] However, existing technologies still have significant limitations: the liquid feeding, separation, detection, and recognition units of most detectors are independent of each other and lack a coordinated design, which leads to a mismatch between the stability of mobile phase transport, the accuracy of grating wavelength adjustment, and the spectral recognition efficiency, and easily causes problems such as detection baseline drift and wavelength deviation. At the same time, grating adjustment and optical path environment control are mostly step-by-step operations, which cannot achieve synchronous linkage and make it difficult to maintain the consistency of the optical path environment during the detection process, thus affecting the repeatability and accuracy of detection data and failing to meet the core requirements of high-precision analysis scenarios for the comprehensive performance of the equipment. Summary of the Invention

[0004] The purpose of this section is to outline some aspects of embodiments of the present invention and to briefly describe some preferred embodiments. Simplifications or omissions may be made in this section, as well as in the abstract and title of this application, to avoid obscuring the purpose of these documents; however, such simplifications or omissions should not be construed as limiting the scope of the invention.

[0005] In view of the problems existing in the current high performance liquid chromatograph ultraviolet detectors, the present invention is proposed.

[0006] Therefore, the purpose of this invention is to provide a high-performance liquid chromatograph ultraviolet detector.

[0007] To solve the above-mentioned technical problems, the present invention provides the following technical solution: a high-performance liquid chromatograph (HPLC) ultraviolet detector, comprising: a main detector housing; a liquid inlet assembly, including a mobile phase module disposed at the upper end of the main detector housing, a liquid delivery component connected to the mobile phase module, and a liquid collection component disposed on the liquid delivery component; a separation and detection assembly, including a chromatographic component connected to the liquid collection component, a flow cell connected to the chromatographic component, and an ultraviolet detection module disposed on the flow cell, wherein the chromatographic component includes a detection chamber disposed within the main detector housing, an ultraviolet light emitter disposed within the detection chamber, a frame disposed at the front end of the ultraviolet light emitter, a grating plate disposed on the frame, and an adjustment component disposed on the frame; and an identification component disposed on the ultraviolet detection module.

[0008] As a preferred embodiment of the ultraviolet detector of the high performance liquid chromatograph of the present invention, the mobile phase module includes a liquid storage tank disposed on the upper end of the main detector housing, a mobile phase delivery pipe connected to the liquid storage tank, and a degassing component disposed on the mobile phase delivery pipe. The liquid delivery component includes a first delivery pipe connected to the degassing component. The first delivery pipe is connected to a liquid collection component. A liquid pump is disposed on the first delivery pipe. A second delivery pipe is disposed on the liquid pump. The second delivery pipe is connected to the chromatographic component.

[0009] As a preferred embodiment of the ultraviolet detector of the high performance liquid chromatograph of the present invention, the liquid collection component includes an autosampler disposed on the main detector housing, a liquid collection tube disposed on the autosampler, and a connecting tube disposed at the upper end of the liquid collection tube, wherein the connecting tube is connected to the first delivery tube.

[0010] As a preferred embodiment of the ultraviolet detector of the high performance liquid chromatograph of the present invention, the ultraviolet detection module is arranged opposite to the ultraviolet light emitting element, the ultraviolet light emitting element is arranged facing the flow cell, the flow cell is provided with a liquid flow channel, and the grating plate is provided with an auxiliary grating element.

[0011] As a preferred embodiment of the high performance liquid chromatograph ultraviolet detector of the present invention, the adjustment component includes a transmission wheel connected to the lower end of each grating plate, a rotating shaft disposed on a plurality of transmission wheels, a drive shaft disposed on a frame, a first bevel gear disposed on each rotating shaft, and a second bevel gear disposed on the drive shaft and meshing with a plurality of first bevel gears. A driven wheel is disposed on the drive shaft, and a drive wheel for driving the driven wheel to rotate is disposed on the frame.

[0012] As a preferred embodiment of the ultraviolet detector of the high performance liquid chromatograph described in this invention, the driven wheel is provided with a plurality of mating arc grooves that cooperate with the outer edge of the driving wheel, the driven wheel is provided with a plurality of elongated grooves, the driving wheel extends a protruding rod, the protruding rod is provided with a mating strip that cooperates with the elongated groove, and the frame is provided with a motor that drives the driving wheel to rotate.

[0013] As a preferred embodiment of the high performance liquid chromatograph ultraviolet detector of the present invention, the auxiliary grating component includes a guide plate disposed on the side of the grating plate, an air guide plate rotatably connected to the guide plate, and a rotating component disposed between the guide plate and the air guide plate. The guide plate is provided with a driving component. The rotating component includes an inclined plate disposed on the guide plate, an extended clamping plate disposed on the inclined plate, a corresponding inclined surface opened on the air guide plate corresponding to the inclined plate, and a mating clamping plate disposed on the corresponding inclined surface. A hinge shaft is provided between the extended clamping plate and the mating clamping plate.

[0014] As a preferred embodiment of the ultraviolet detector of the high performance liquid chromatograph described in this invention, the driving component includes a threaded rod disposed on a guide plate, a driving block slidably connected to the guide plate, a threaded hole opened on the driving block, a first hinged ball disposed on the driving block, a second hinged ball disposed on the air guide plate, and a hinged rod disposed between the first hinged ball and the second hinged ball.

[0015] As a preferred embodiment of the ultraviolet detector of the high performance liquid chromatograph described in this invention, the threaded rod end is provided with a drive motor, and the drive motor and the threaded rod are connected by two meshing gears.

[0016] As a preferred embodiment of the ultraviolet detector of the high performance liquid chromatograph described in this invention, the guide plate is provided with an arc-shaped cover.

[0017] The beneficial effects of this invention are as follows: The main detector housing, liquid inlet assembly, separation and detection assembly, and identification assembly work together to achieve stable delivery of the mobile phase, accurate sample injection, chromatographic separation, ultraviolet quantitative detection, and spectral data identification and analysis. It specifically solves the problems of low wavelength adjustment accuracy and lack of linkage between grating adjustment and optical path environmental control in traditional ultraviolet detectors. It can be adapted to the high-performance liquid chromatography qualitative and quantitative detection needs in fields such as drug analysis, environmental monitoring, food quality testing, and chemical synthesis. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Wherein:

[0019] Figure 1 This is a schematic diagram of the overall structure of the ultraviolet detector in the high-performance liquid chromatograph of this invention.

[0020] Figure 2 This is a schematic diagram of the internal structure of the ultraviolet detector in the high-performance liquid chromatograph of this invention.

[0021] Figure 3 This is a schematic diagram of the liquid delivery component of the ultraviolet detector in the high-performance liquid chromatograph of the present invention.

[0022] Figure 4 This is a schematic diagram of the chromatographic component of the ultraviolet detector in the high-performance liquid chromatograph of this invention.

[0023] Figure 5 This is a schematic diagram of the adjustment components of the ultraviolet detector in the high-performance liquid chromatograph of the present invention.

[0024] Figure 6 for Figure 5 Enlarged diagram of part A in the middle.

[0025] Figure 7 This is a schematic diagram of the driven wheel structure of the ultraviolet detector in the high-performance liquid chromatograph of the present invention.

[0026] Figure 8 This is a schematic diagram of the recognition component of the ultraviolet detector in the high-performance liquid chromatograph of the present invention.

[0027] Explanation of reference numerals in the attached drawings: 100, Liquid inlet assembly; 101, Mobile phase module; 102, Liquid delivery component; 103, Liquid collection component; 200, Separation and detection component; 201, Chromatographic component; 202, Flow cell; 203, Ultraviolet detection module; 1011, Storage tank; 1012, Mobile phase delivery tube; 1013, Degassing component; 1021, First delivery tube; 1022, Infusion pump; 1023, Second delivery tube; 1031, Autosampler; 1032, Collection tube; 1033, Connecting tube; 2011, Detection chamber; 2012, Ultraviolet light emitter; 2013, Frame; 2014, Grating plate; 2015, Liquid flow channel; 300, Adjustment component; 301, Transmission wheel; 302, Rotating shaft; 303, Drive spindle; 3 04. First bevel gear; 305. Second bevel gear; 306. Driven gear; 307. Driving gear; 308. Mating arc groove; 309. Long groove; 3091. Protruding rod; 3092. Mating strip; 3093. Motor; 400. Auxiliary grating component; 401. Guide plate; 402. Air guide plate; 404. Rotating component; 4041. Inclined plate; 4042. Extending clamping plate; 4043. Corresponding inclined surface; 4044. Mating clamping plate; 500. Driving component; 501. Threaded rod; 502. Driving block; 504. First hinged ball; 505. Second hinged ball; 506. Hinge rod; 507. Drive motor; 600. Core control unit; 601. Spectral data processing unit; 602. Sample identification unit; 603. Data storage unit and communication unit. Detailed Implementation

[0028] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0029] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0030] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that is mutually exclusive with other embodiments.

[0031] Secondly, the present invention is described in detail with reference to the schematic diagrams. When detailing the embodiments of the present invention, for ease of explanation, the cross-sectional views illustrating the device structure may be partially enlarged, not according to the usual scale. Furthermore, the schematic diagrams are merely examples and should not limit the scope of protection of the present invention. In addition, actual fabrication should include three-dimensional spatial dimensions of length, width, and depth.

[0032] Example 1

[0033] Reference Figures 1-8 This first embodiment of the invention provides a high-performance liquid chromatograph (HPLC) ultraviolet (UV) detector, including a main detector housing, a liquid inlet assembly 100, a separation and detection assembly 200, and an identification assembly. These units work in synergy to achieve stable mobile phase delivery, accurate sample injection, chromatographic separation, UV quantitative detection, and spectral data identification and analysis. It specifically addresses the problems of low wavelength adjustment accuracy and the inability to link grating adjustment with optical path environmental control in traditional UV detectors. This invention is suitable for the qualitative and quantitative detection needs of HPLC in fields such as drug analysis, environmental monitoring, food quality testing, and chemical synthesis.

[0034] Furthermore, the main detector housing serves as the basic support and protection unit for the equipment, providing a sealed, stable, and light-proof installation environment for the internal flow path, optical path, and electrical components. In this embodiment, the main detector housing adopts an integrated die-cast aluminum alloy vertical box structure. The main detector housing has a uniform wall thickness, and the inner wall is sprayed with a matte black light-absorbing coating, which can completely isolate the interference of external ambient light and prevent stray light from entering the optical path system and causing the detection baseline to drift.

[0035] Preferably, the bottom of the main detector housing is provided with four support feet with anti-slip pads, which can be adjusted to ensure the horizontal installation accuracy of the internal flow path and optical path.

[0036] Furthermore, the present invention also includes a liquid inlet assembly 100, which is a sample and mobile phase delivery unit for the detector, realizing mobile phase degassing, constant pressure stable delivery, and accurate quantitative collection and injection of samples, providing a stable flow path basis for subsequent chromatographic separation and ultraviolet detection. In this embodiment, the liquid inlet assembly 100 includes a mobile phase module 101 disposed on the upper end of the main detector housing, a liquid delivery component 102 connected to the mobile phase module 101, and a liquid collection component 103 disposed on the liquid delivery component 102.

[0037] Furthermore, the mobile phase module 101 includes a storage tank 1011 fixed to the upper end of the main detector housing, a mobile phase delivery pipe 1012 connected to the bottom outlet of the storage tank 1011, and a degassing component 1013 connected in series with the mobile phase delivery pipe 1012. The storage tank 1011 is made of brown borosilicate glass, which can prevent the organic components in the mobile phase from decomposing when exposed to light. A sealing cap and a vent valve are provided at the upper end of the storage tank 1011. The vent valve has an organic phase filter membrane built in, which can ensure the pressure balance inside and outside the tank and prevent dust and oxygen in the air from entering the mobile phase. The degassing component 1013 adopts an online vacuum degasser. The degasser includes a degassing chamber, a polytetrafluoroethylene degassing pipeline wound in the degassing chamber, and a miniature vacuum pump connected to the degassing chamber. It can remove dissolved gases in the mobile phase online and prevent bubbles from entering the detection flow path, which would cause baseline fluctuations and abnormal detection peaks.

[0038] Furthermore, the liquid delivery component 102 includes a first delivery pipe 1021 connected to the outlet of the degassing component 1013. The outlet end of the first delivery pipe 1021 is connected to the liquid collection component 103. A liquid pump 1022 is connected in series in the first delivery pipe 1021. A second delivery pipe 1023 is connected to the outlet end of the liquid pump 1022. The end of the second delivery pipe 1023 is connected to the inlet of the chromatographic column of the chromatographic component 201.

[0039] Preferably, the infusion pump 1022 is a dual-plunger series constant flow infusion pump, which can realize pulse-free constant pressure delivery of the mobile phase. An online filter and a pressure sensor are also connected in series at the outlet of the infusion pump 1022. The online filter can filter out small particulate impurities in the mobile phase and protect the chromatographic column and subsequent flow path. The pressure sensor can monitor the pressure of the flow path system in real time and automatically trigger an alarm and shutdown protection when the pressure exceeds the limit.

[0040] Furthermore, the liquid collection component 103 includes an autosampler 1031, a collection tube 1032 disposed on the autosampler 1031, and a connecting tube 1033 disposed on the upper end of the collection tube 1032. The outlet end of the connecting tube 1033 is connected to the degassing component 1013, and the inlet end of the first delivery tube 1021 is also connected to the degassing component 1013. The outlet end of the collection tube 1032 is connected to a six-way injection valve. The six-way injection valve is a high-precision quantitative ring injection valve, which can realize accurate quantitative injection of samples. The quantitative ring can be replaced with different specifications according to the detection requirements to adapt to the injection volume requirements of 1μL-100μL.

[0041] Preferably, the autosampler 1031 is also equipped with a sample tray, a high-precision injection needle, a needle holder, and a needle washing unit, all of which use common equipment in the existing conventional fields. The sample tray can hold multiple sample vials, and the sample position can be accurately switched by a stepper motor. The injection needle is driven by a three-dimensional motion guide rail, which can accurately insert into the sample vial to extract the sample, and then inject it into the quantitative loop of the six-way injection valve. The needle washing unit includes a needle washing bottle and a peristaltic pump, which can clean the inner and outer walls of the injection needle before and after injection.

[0042] The degassing component 1013 of the mobile phase module 101 can reduce the dissolved oxygen content in the mobile phase to below 1 ppm, completely avoiding detection spikes and baseline jumps caused by bubbles entering the flow path and flow cell 202, thus ensuring the stability of the detection baseline. The dual-plunger series infusion pump 1022 achieves pulse-free constant flow delivery of the mobile phase, with high flow accuracy and good repeatability, ensuring stable mobile phase flow rate during chromatographic separation and significantly improving the accuracy of qualitative detection. The autosampler 1031 enables fully automated quantitative sample collection and injection, avoiding errors from manual operation and significantly improving detection efficiency and data repeatability. The multi-stage filtration design of the flow path system protects the chromatographic column and detection flow path, and also prevents particulate impurities from entering the flow cell 202, which could cause light path obstruction and detection noise.

[0043] Furthermore, the separation and detection component 200 is the core functional unit of the detector, including a chromatographic component 201 connected to the liquid collection component 103, a flow cell 202 connected to the chromatographic component 201, and an ultraviolet detection module 203 disposed on the flow cell 202, realizing chromatographic separation of the sample, ultraviolet light absorption detection and photoelectric signal conversion, which is the core of realizing qualitative and quantitative analysis of the sample.

[0044] In this embodiment, the chromatographic component 201 includes a detection chamber 2011 disposed within the optical path detection cavity of the main detector housing, a chromatographic column holder fixed at the inlet of the detection chamber 2011, and a chromatographic column mounted on the chromatographic column holder. The inlet end of the chromatographic column is connected to the second delivery pipe 1023 via a pipeline, and the outlet end is connected to the liquid inlet of the flow cell 202 via a pipeline. The chromatographic column is made of stainless steel and filled with stationary phase packing material. Different types of packing material can be selected according to the properties of the sample to achieve efficient chromatographic separation of different components in the sample. A constant temperature heating jacket is provided on the chromatographic column holder to precisely control the temperature of the chromatographic column and avoid fluctuations in chromatographic separation effect caused by changes in ambient temperature.

[0045] The specific structure of the flow cell 202: The flow cell 202 adopts a Z-shaped flow cell structure and is fixed at the center of the optical path of the detection chamber 2011. A liquid flow channel 2015 is opened inside the flow cell 202. The inlet of the liquid flow channel 2015 is connected to the outlet of the chromatographic column, and the outlet is connected to the waste liquid pipe. The liquid flow channel 2015 adopts a precision-machined cylindrical flow channel, and the inner wall is polished to reduce sample residue and diffusion.

[0046] The flow cell 202 is made of stainless steel and is wrapped with a thermostatic jacket. It is linked to the thermostatic system of the chromatographic column to maintain a constant temperature of the sample solution in the flow cell 202, avoiding changes in sample refractive index and baseline drift caused by temperature changes. The inlet and outlet of the flow cell 202 both adopt a conical flow guide structure, which can reduce sample diffusion in the flow channel, reduce peak broadening, and improve the resolution and detection sensitivity of chromatographic peaks.

[0047] Furthermore, the chromatography component 201 also includes an ultraviolet light emitter 2012 disposed within the detection chamber 2011, a frame 2013 fixed within the detection chamber 2011, a grating plate 2014 mounted on the frame 2013, and an ultraviolet detection module 203 electrically connected to the ultraviolet light emitter 2012. The ultraviolet detection module 203 is disposed opposite to the ultraviolet light emitter 2012. The light outlet of the ultraviolet light emitter 2012 is positioned directly opposite the quartz optical window of the flow cell 202. The ultraviolet light emitter 2012 is a combination light source of deuterium lamp and tungsten lamp. The deuterium lamp can emit ultraviolet light of 190-400nm, and the tungsten lamp can emit visible light of 400-700nm, which can cover the detection requirements of the entire ultraviolet-visible light band. The light outlet of the light source is provided with a condenser lens group, which can collimate the divergent light emitted by the light source into a parallel beam and project it onto the grating plate 2014.

[0048] The grating plate 2014 can disperse parallel composite light into monochromatic light of different wavelengths. The grating plate 2014 is rotatably connected to the frame 2013 via a rotating shaft. The diffraction angle of the grating plate 2014 can be adjusted by rotating the shaft, thereby selecting monochromatic light of different wavelengths to be projected onto the flow cell 202. An auxiliary grating element 400 is provided on the side of the grating plate 2014.

[0049] The ultraviolet detection module 203 is a photodiode array detector, located on the light-emitting side of the flow cell 202. It can receive ultraviolet light after penetrating the sample solution and convert the optical signal into an electrical signal. The photodiode array detector can simultaneously acquire spectral data in the full wavelength range of 190-700nm, realizing full-wavelength scanning and simultaneous detection of multiple wavelengths of the sample. The ultraviolet detection module 203 also includes a signal amplification circuit, an analog-to-digital conversion circuit, and a data transmission circuit, which can amplify and convert the weak electrical signal after photoelectric conversion to analog-to-digital, and then transmit it to the recognition component and the host computer for data processing and analysis.

[0050] The isothermal control and high-efficiency column of the chromatographic component 201 enable efficient separation of different components in the sample, improving the resolution by more than 30%, laying the foundation for subsequent UV quantitative detection. The Z-shaped flow cell 202 design minimizes sample diffusion and peak broadening while ensuring the detection optical path, significantly improving detection sensitivity and achieving a minimum detection limit at the ng level. The combination of deuterium and tungsten lamps covers the entire UV-Vis light band, adapting to the detection needs of samples with different UV absorption characteristics and greatly expanding its applicability. The planar holographic grating plate 2014 has high dispersion accuracy and low stray light. Combined with the rotatable mounting structure, it enables continuous adjustment of the detection wavelength to meet the characteristic wavelength detection requirements of different samples.

[0051] Furthermore, the adjustment component 300 is a wavelength adjustment drive unit for the grating plate 2014, realizing high-precision rotation of the grating plate 2014 and precise adjustment of the detection wavelength, ensuring the accuracy and repeatability of wavelength adjustment. In this embodiment, the adjustment component 300 includes a transmission wheel 301 coaxially fixedly connected to the lower end of each grating plate 2014, a rotating shaft 302 passing through the center of several transmission wheels 301, a drive shaft 303 rotatably connected to the frame 2013, a first bevel gear 304 fixed on each rotating shaft 302, and several second bevel gears 305 fixed on the drive shaft 303. Each second bevel gear 305 meshes with the corresponding first bevel gear 304.

[0052] The transmission wheel 301 is rigidly connected to the rotating shaft of the grating plate 2014 on the same axis. A three-dimensional support is provided on the frame 2013. The three-dimensional support is rotatably connected to the rotating shaft 302 through a precision bearing and is set coaxially with the rotating shaft of the grating plate 2014 to ensure that the rotation angle is completely synchronized with the diffraction angle of the grating plate 2014.

[0053] Preferably, the drive spindle 303 is rotatably connected to the frame 2013 via two sets of angular contact ball bearings and is arranged spatially perpendicular to the rotating shaft 302. The second bevel gear 305 on the drive spindle 303 and the first bevel gear 304 on the rotating shaft 302 form a right-angle transmission mechanism. The transmission ratio can be designed according to the wavelength adjustment accuracy requirements to achieve precise transmission of the rotation of the drive spindle 303 to the rotation of the grating plate 2014 shaft. A driven wheel 306 is fixedly installed at one end of the drive spindle 303, and a driving wheel 307 that cooperates with the driven wheel 306 is rotatably connected to the frame 2013. A motor 3093 that drives the driving wheel 307 to rotate is fixed on the side wall of the frame 2013.

[0054] Furthermore, the driven wheel 306 has several circumferentially arranged grooves 308 that mate with the outer edge of the driving wheel 307. The driven wheel 306 also has several elongated grooves 309 arranged in a circumferential array on its end face. A protruding rod 3091 extends from the end face of the driving wheel 307, and a mating strip 3092 that mates with the elongated groove 309 is fixed to the end of the protruding rod 3091. The driving wheel 307 is an incomplete gear-type drive wheel. The mating grooves 308 fit snugly against the outer edge of the driving wheel 307, locking the driven wheel 306 during non-transmission phases to prevent accidental rotation. When the driving wheel 307 rotates, the mating strip 3092 first engages in the elongated groove 309, causing the driven wheel 306 to rotate by a set angle. Subsequently, the mating strip 3092 disengages from the elongated groove 309, and the outer edge of the driving wheel 307 engages and locks with the mating groove 308, achieving intermittent and precise rotation of the driven wheel 306.

[0055] The bevel gear transmission mechanism, consisting of the first bevel gear 304 and the second bevel gear 305, enables synchronous linkage between the drive spindle 303 and multiple grating plates 2014. It can simultaneously drive multiple sets of grating plates 2014 to rotate synchronously, ensuring the consistency of wavelength adjustment during multi-channel detection. The motor 3093 drives the rotation of the drive wheel 307 and the driven wheel 306. By utilizing the intermittent engagement of the mating strip 3092 and the long groove 309, it achieves high-precision intermittent rotation of the grating plate 2014. The incomplete gear-type intermittent transmission structure can mechanically lock the driven wheel 306 and the grating plate 2014 during the non-adjustment stage, preventing vibration during equipment operation from causing angular displacement of the grating plate 2014, ensuring the stability of wavelength during detection, and reducing baseline drift and detection errors.

[0056] Furthermore, an auxiliary grating component 400 is provided on the grating plate 2014. The auxiliary grating component 400 includes a guide plate 401 fixed to the side of the grating plate 2014, an air guide plate 402 rotatably connected to the guide plate 401, and a rotating component 404 disposed between the guide plate 401 and the air guide plate 402. The guide plate 401 is fixed to the side of the grating plate 2014 by bolts and rotates synchronously with the grating plate 2014, providing an installation reference for the air guide plate 402. The air guide plate 402 has an arc-shaped plate structure, with its inner arc surface facing the grating surface of the grating plate 2014.

[0057] Preferably, a number of energy-saving air guide components are provided on the air guide plate 402. The energy-saving air guide components are arranged in an equidistant array along the length direction of the air guide plate 402. The energy-saving air guide components are miniature airflow nozzles that can be connected to a clean and dry air source through pipelines. The sprayed clean airflow can form a uniform air curtain along the grating surface, which can not only remove the small amount of heat generated when the grating is working and maintain the grating temperature constant, but also prevent dust in the air from adhering to the grating lines, thus avoiding a decrease in grating transmittance and an increase in stray light.

[0058] In this embodiment, the rotating component 404 includes an inclined plate 4041 integrally formed on the end of the guide plate 401, an extended clamping plate 4042 fixed on the inclined plate 4041, a corresponding inclined surface 4043 corresponding to the inclined plate 4041 and formed on the end of the air guide plate 402, and a mating clamping plate 4044 fixed on the corresponding inclined surface 4043. The extended clamping plate 4042 and the mating clamping plate 4044 are rotatably connected by a hinge shaft. The tilt angle of the inclined plate 4041 matches the initial installation angle of the air guide plate 402. The hinge shaft adopts a precision micro hinge, which can realize the 0-90° angle adjustment of the air guide plate 402 relative to the guide plate 401, thereby adjusting the jet angle of the airflow nozzle and adapting to the air curtain coverage requirements of the grating plate 2014 at different rotation angles.

[0059] Furthermore, the driving component 500 includes a threaded rod 501 rotatably connected to the guide plate 401, a driving block 502 slidably connected to the guide plate 401, a threaded hole in the driving block 502 that mates with the threaded rod 501, a first hinged ball 504 fixed to the end of the driving block 502, a second hinged ball 505 fixed to the back of the air guide plate 402, and a hinged rod 506 with its two ends respectively hinged to the two hinged balls; the threaded rod 501 is rotatably connected to the guide plate 401 through a bearing seat, and together with the driving block 502, forms a screw and nut transmission mechanism. When the threaded rod 501 rotates, it can drive the driving block 502 to slide linearly on the guide plate 401; the first hinged ball 504 and the second hinged ball 505 are both universal ball joint structures, which can adapt to the angle changes during the rotation of the air guide plate 402, avoid transmission jamming, and ensure that the rotation of the threaded rod 501 can be accurately converted into the angle adjustment of the air guide plate 402.

[0060] Furthermore, a drive motor 507 is provided at the end of the threaded rod 501, and the drive motor 507 and the threaded rod 501 are connected by two meshing gears.

[0061] Preferably, an arc-shaped cover is provided on the guide plate 401, which is used to enclose the entire drive component 500.

[0062] The air guide plate 402 and airflow nozzle of the auxiliary grating component 400 can form a uniform clean air curtain on the grating surface. This can maintain a constant grating temperature, control the temperature fluctuation of the grating plate 2014 within ±0.2℃, significantly reduce wavelength drift caused by thermal expansion and contraction of the grating, and prevent dust and moisture from adhering to the grating lines, avoiding grating performance degradation and extending the grating's service life. At the same time, it can reduce stray light and improve the signal-to-noise ratio of the detection. The screw-nut drive + universal ball joint structure of the rotating component 404 and the driving component 500 can realize precise adjustment of the angle of the air guide plate 402 and can be synchronized with the wavelength adjustment action of the grating plate 2014. When the grating plate 2014 rotates to adjust the wavelength, the air guide plate 402 adjusts its angle synchronously to ensure that the air curtain always completely covers the grating surface, ensuring the continuity of temperature control and dust prevention effects.

[0063] The identification component is the spectral data processing and analysis unit of the detector, which realizes the acquisition, processing, spectral identification and analysis of photoelectric signals, and finally outputs the qualitative and quantitative detection results of the sample. The identification component is set in the electrical control cavity of the main detector housing and is electrically connected to the ultraviolet detection module 203. It includes a core control unit 600, a spectral data processing unit 601, a sample identification unit 602, a data storage unit and a communication unit 603. The core control unit 600 uses a high-performance ARM processor to provide the computing and control core for the entire identification component; the spectral data processing unit 601 includes a filtering circuit, a baseline correction module, and a spectral smoothing module, which can perform noise reduction, baseline correction, and smoothing on the acquired photoelectric signals to remove noise and interference and improve the quality of spectral data; the sample identification unit 602 has a built-in spectral database and matching algorithm, which can compare the detected sample spectrum with the standard spectrum in the database to achieve qualitative identification and purity analysis of the sample; the data storage unit uses a large-capacity solid-state drive to store detection methods, spectral data, detection results, and equipment operation logs; the communication unit includes communication interfaces such as Ethernet, USB, and RS232, which can realize data transmission and remote control with host computers and chromatography workstations.

[0064] The identification component can transmit the chromatogram and spectrum of the sample in real time, automatically calculate the retention time, peak area, and peak height of the chromatographic peaks, and complete the quantitative calculation of the sample through external standard and internal standard methods; it can perform full-wavelength spectral scanning of chromatographic peaks, extract peak purity information, determine whether the chromatographic peak is a single component, and identify co-eluting impurities; it supports user-defined detection methods and spectral databases, and can establish standard curves and qualitative identification libraries according to detection needs, adapting to the detection needs of different industries.

[0065] Operation process: Connect the power supply to the equipment, start the control system of the ultraviolet detector, turn on the deuterium lamp and tungsten lamp light source, and preheat the light source for ≥30 minutes to ensure stable light emission; at the same time, start the constant temperature control system of the chromatographic column and flow cell 202 to raise the temperature to the set value and maintain it stable; start the online vacuum degasser of the mobile phase module 101 to degas the mobile phase.

[0066] Then, start the infusion pump 1022 to deliver the mobile phase at the set flow rate, flushing the entire flow path system, including the chromatographic column, flow cell 202 and tubing, until the flow path system is free of air bubbles and the baseline is stable; according to the properties of the sample to be tested, set the flow rate and ratio of the mobile phase to complete the balancing of the chromatographic system until the baseline noise and drift meet the detection requirements.

[0067] Then, through the operation interface of the identification component, the wavelength required for detection is set, the control system starts the motor 3093, drives the active wheel 307 and the driven wheel 306 to rotate, and drives the grating plate 2014 to rotate to the set angle through the bevel gear transmission to complete the wavelength adjustment; at the same time, the drive component 500 starts synchronously, adjusts the air guide plate 402 to the corresponding angle, opens the airflow nozzle, and forms an air curtain on the grating surface to maintain the stability of the grating environment.

[0068] The sample to be tested is placed in the sample tray of the autosampler 1031, the injection volume and injection program are set, and the injection process is started. The autosampler 1031 accurately extracts the sample and injects it into the quantitative loop of the six-way injection valve. Then the six-way valve is switched, and the mobile phase carries the sample in the quantitative loop into the chromatographic column. Different components in the sample are separated in the chromatographic column and flow out sequentially with the mobile phase into the flow cell 202.

[0069] Then, the composite light emitted by the ultraviolet light emitter 2012 is dispersed into monochromatic light of a set wavelength by the grating plate 2014, and penetrates the sample solution in the flow cell 202. The components in the sample produce characteristic absorption of ultraviolet light, and the transmitted light signal is received by the ultraviolet detection module 203 and converted into an electrical signal. The identification component processes, collects and stores the electrical signal in real time, continuously transmits the chromatogram, and simultaneously collects the full wavelength spectral data of the sample components.

[0070] After sample testing is completed, the identification component automatically processes the collected chromatographic and spectral data, calculates the retention time and peak area of ​​each component, and completes quantitative calculation; through spectral comparison, it completes the qualitative identification and purity analysis of the components, and generates and outputs test reports, chromatograms, and spectra.

[0071] It is important to note that the constructions and arrangements of this application shown in several different exemplary embodiments are merely illustrative. Although only a few embodiments are described in detail in this disclosure, those who consult this disclosure will readily understand that many modifications are possible (e.g., changes in the size, dimensions, structure, shape, and proportions of various elements, as well as parameter values ​​(e.g., temperature, pressure, etc.), installation arrangements, use of materials, color, orientation, etc.) without substantially departing from the novel teachings and advantages of the subject matter described in this application. For example, an element shown as integrally formed may be composed of multiple parts or elements, the position of elements may be inverted or otherwise altered, and the nature or number or position of discrete elements may be changed or altered. Therefore, all such modifications are intended to be included within the scope of the invention. The order or sequence of any process or method steps may be changed or rearranged according to alternative embodiments. In the claims, any "device plus function" clause is intended to cover the structure described herein that performs the function, and not only structurally equivalent but also equivalent in structure. Other substitutions, modifications, alterations, and omissions may be made in the design, operation, and arrangement of the exemplary embodiments without departing from the scope of the invention. Therefore, the present invention is not limited to the specific embodiments, but extends to various modifications that still fall within the scope of the appended claims.

[0072] Furthermore, in order to provide a concise description of exemplary embodiments, not all features of actual embodiments (i.e., those features that are not relevant to the best mode of carrying out the invention as currently considered, or those features that are not relevant to implementing the invention) may be omitted.

[0073] It should be understood that numerous specific implementation decisions can be made during the development of any practical implementation, such as in any engineering or design project. Such development efforts may be complex and time-consuming, but for those skilled in the art who benefit from this disclosure, the development effort will be a routine work of design, manufacturing, and production without requiring much experimentation.

[0074] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A high-performance liquid chromatograph ultraviolet detector, characterized in that: Includes the main detector housing; The liquid inlet assembly (100) includes a mobile phase module (101) disposed on the upper end of the main detector housing, a liquid delivery component (102) connected to the mobile phase module (101), and a liquid collection component (103) disposed on the liquid delivery component (102). The separation and detection assembly (200) includes a chromatographic component (201) connected to a liquid collection component (103), a flow cell (202) connected to the chromatographic component (201), and an ultraviolet detection module (203) disposed on the flow cell (202). The chromatographic component (201) includes a detection chamber (2011) disposed in the main detector housing, an ultraviolet light emitter (2012) disposed in the detection chamber (2011), a frame (2013) disposed at the front end of the ultraviolet light emitter (2012), a grating plate (2014) disposed on the frame (2013), and an adjustment component (300) disposed on the frame (2013). An identification component is disposed on the ultraviolet detection module (203); The mobile phase module (101) includes a liquid storage tank (1011) disposed on the upper end of the main detector housing, a mobile phase delivery pipe (1012) connected to the liquid storage tank (1011), and a degassing component (1013) disposed on the mobile phase delivery pipe (1012). The liquid delivery component (102) includes a first delivery pipe (1021) connected to the degassing component (1013). The first delivery pipe (1021) is connected to the liquid collection component (103). A liquid pump (1022) is disposed on the first delivery pipe (1021), and a second delivery pipe is disposed on the liquid pump (1022). (1023), the second delivery pipe (1023) is connected to the chromatography component (201); the ultraviolet detection module (203) is arranged opposite to the ultraviolet light emitter (2012), the ultraviolet light emitter (2012) is arranged facing the flow cell (202), the flow cell (202) is provided with a liquid flow channel (2015), and an auxiliary grating component (400) is provided on the grating plate (2014); the adjustment component (300) includes a transmission wheel (301) connected to the lower end of each grating plate (2014) and a rotating shaft (302) arranged on several transmission wheels (301). The frame (2013) includes a drive shaft (303), a first bevel gear (304) on each rotating shaft (302), and a second bevel gear (305) on the drive shaft (303) meshing with several first bevel gears (304). A driven wheel (306) is mounted on the drive shaft (303), and a drive wheel (307) on the frame (2013) drives the driven wheel (306) to rotate. The auxiliary grating component (400) includes a guide plate (401) mounted on the side of the grating plate (2014) and a wind deflector (405) rotatably connected to the guide plate (401). 2) A rotating component (404) is disposed between the guide plate (401) and the air guide plate (402). The guide plate (401) is provided with a driving component (500). The rotating component (404) includes an inclined plate (4041) disposed on the guide plate (401), an extended clamping plate (4042) disposed on the inclined plate (4041), a corresponding inclined surface (4043) on the air guide plate (402) corresponding to the inclined plate (4041), and a mating clamping plate (4044) disposed on the corresponding inclined surface (4043). A hinge shaft is provided between the extended clamping plate (4042) and the mating clamping plate (4044).

2. The high-performance liquid chromatograph ultraviolet detector as described in claim 1, characterized in that: The liquid collection component (103) includes an autosampler (1031) mounted on the main detector housing, a liquid collection tube (1032) mounted on the autosampler (1031), and a connecting tube (1033) mounted on the upper end of the liquid collection tube (1032). The connecting tube (1033) is connected to the degassing component (1013).

3. The high-performance liquid chromatograph ultraviolet detector as described in claim 1, characterized in that: The driven wheel (306) has several mating arc grooves (308) arranged in an array to cooperate with the outer edge of the driving wheel (307). The driven wheel (306) has several elongated grooves (309) arranged in an array. The driving wheel (307) has a protruding rod (3091) extending out. The protruding rod (3091) is provided with a mating strip (3092) that cooperates with the elongated groove (309). The frame (2013) is provided with a motor (3093) that drives the driving wheel (307) to rotate.

4. The high-performance liquid chromatograph ultraviolet detector as described in claim 1, characterized in that: The drive unit (500) includes a threaded rod (501) disposed on the guide plate (401), a drive block (502) slidably connected to the guide plate (401), a threaded hole opened on the drive block (502), a first hinged ball (504) disposed on the drive block (502), a second hinged ball (505) disposed on the air guide plate (402), and a hinge rod (506) disposed between the first hinged ball (504) and the second hinged ball (505).

5. The high-performance liquid chromatograph ultraviolet detector as described in claim 4, characterized in that: The threaded rod (501) is equipped with a drive motor (507) at its end, and the drive motor (507) is connected to the threaded rod (501) through two meshing gears.

6. The high-performance liquid chromatograph ultraviolet detector as described in claim 4, characterized in that: An arc-shaped cover is provided on the guide plate (401).

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