Oxidoreductase-containing composition for cosmetics as well as preparation method and application of oxidoreductase-containing composition

Abstract

The invention relates to the field of cosmetic raw material development and cosmetics, in particular to a cosmetic composition containing oxidoreductase as well as a preparation method and application of the cosmetic composition. The composition is prepared from the following components in parts by weight: 1.5 to 3.5 parts of oxidoreductase, 0.3 to 2.5 parts of aloe barbadensis leaf extract, 0.5 to 3 parts of pimpinella diversifolia fruit extract, 0.6 to 4 parts of lysozyme, 0.8 to 2.5 parts of papain, 0.5 to 1.8 parts of lysozyme, 0.5 to 3.5 parts of pansy extract and 0.5 to 3 parts of amur corktree bark extract. The composition prepared by the invention has excellent efficiency of inhibiting propionibacterium acnes and elastinase, and has the effects of removing acnes, tightening skin and resisting aging; the composition uses active components for moisturizing, anti-inflammation, sterilization and the like, and also has good effects of sensitive repair and skin micro-ecology regulation.

Description

Technical Field

[0001] This invention relates to the development of cosmetic raw materials and the field of cosmetics, specifically to a cosmetic composition containing an oxidoreductase, its preparation method, and its application. Background Technology

[0002] Enzymes, as biological catalysts, play a vital role in the cosmetics industry. Their core advantages lie in their high efficiency and specificity, accelerating specific biochemical reactions under gentle conditions. In exfoliation, proteases (such as papain) precisely sever the connecting proteins between aging keratinocytes, gently removing dead skin cells, unclogging pores, and improving dullness, while avoiding the mechanical damage to the skin caused by physical scrubs. In functional skincare, lysozyme hydrolyzes bacterial cell walls, aiding in antibacterial and acne-reducing effects; superoxide dismutase (SOD) is a powerful antioxidant that scavenge free radicals to delay photoaging. Furthermore, enzymes promote skin metabolism and microcirculation, enhancing the skin's absorption of nutrients, resulting in smooth, radiant, and healthy skin—a key ingredient in building gentle yet effective skincare systems.

[0003] Plant extracts play an irreplaceable role in cosmetics due to their synergistic effects across multiple components and targets. Rich in polysaccharides, flavonoids, polyphenols, and amino acids, plant extracts offer a multitude of uses. For moisturizing and repairing, extracts like aloe vera and oat are rich in hydrophilic polysaccharides, deeply locking in moisture and repairing damaged skin barriers. For soothing and anti-inflammatory effects, ingredients like chamomile and centella asiatica effectively inhibit the release of inflammatory factors, relieving redness and sensitivity. Furthermore, plant extracts are key players in anti-oxidation and whitening; ingredients like green tea and licorice root extract can scavenge free radicals and inhibit tyrosinase activity, thus brightening the complexion. Because they are derived from nature and are relatively gentle, plant extracts not only provide products with superior efficacy but also enhance safety and skin feel, meeting consumers' pursuit of green skincare.

[0004] Although there is a wide variety of cosmetic ingredients containing enzymes and plant extracts, their functions are relatively limited, and their efficacy still needs improvement. Therefore, providing a cosmetic ingredient with comprehensive functions and excellent efficacy remains one of the technical problems that need to be solved. Summary of the Invention

[0005] In order to solve the technical problems described in the background art, the present invention proposes a technical solution: a cosmetic composition containing an oxidoreductase, its preparation method and application, which relates to the development of cosmetic raw materials and the cosmetic field.

[0006] One aspect of this invention provides a cosmetic composition containing an oxidoreductase, the raw materials of which are: an oxidoreductase, aloe vera leaf extract, fennel fruit extract, golden button flower extract, papain, lysozyme, pansy extract, and phellodendron bark extract.

[0007] Optionally, the oxidoreductase includes at least one of lactate dehydrogenase, succinate dehydrogenase, cytochrome oxidase, catalase, and peroxidase.

[0008] Optionally, the weight ratio of anise fruit extract to Phellodendron bark extract is 1:0.5-1.2.

[0009] Optionally, the anise fruit extract is obtained by first pulverizing the fruit, then extracting it with 80-95 wt% ethanol, centrifuging, filtering, concentrating to remove the ethanol, adding ethyl acetate for extraction, and concentrating.

[0010] Optionally, the extract of the golden button flower is obtained by first pulverizing the flower, then extracting it with 80-95wt% ethanol, centrifuging, filtering, concentrating to remove the ethanol, adding ethyl acetate for extraction, and concentrating.

[0011] Optionally, the bark extract of Phellodendron amurense is obtained by first pulverizing the bark, then extracting it with 15-30 wt% ethanol, followed by centrifugation, filtration, concentration, and drying.

[0012] Alternatively, the pansy extract is obtained by first pulverizing the pansy, then extracting it with 15-30 wt% ethanol, followed by centrifugation, filtration, concentration, and drying.

[0013] Optionally, a cosmetic composition containing an oxidoreductase comprises, by weight, the following ingredients: 1.5-3.5 parts oxidoreductase, 0.3-2.5 parts aloe vera leaf extract, 0.5-3 parts anise fruit extract, 0.6-4 parts golden button flower extract, 0.8-2.5 parts papain, 0.5-1.8 parts lysozyme, 0.5-3.5 parts pansy extract, and 0.5-3 parts Amur cork tree bark extract.

[0014] Optionally, the amount of oxidoreductase used can also be 1.5, 1.8, 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5 parts or any range of two values.

[0015] Optionally, the dosage of aloe vera leaf extract may be 0.3, 0.5, 0.8, 1, 1.2, 1.5, 1.8, 2, 2.2, 2.5 parts or any range of two values.

[0016] Optionally, the amount of anise fruit extract may be 0.3, 0.5, 0.8, 1, 1.2, 1.5, 1.8, 2, 2.2, 2.5, 2.8, 3 parts or any range of two values.

[0017] Optionally, the amount of golden button flower extract can be 0.6, 0.8, 1, 1.2, 1.5, 1.8, 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4 parts or any range of two values.

[0018] Optionally, the amount of papain used can be 0.8, 1, 1.2, 1.5, 1.8, 2, 2.2, 2.5 parts or any range of two values.

[0019] Optionally, the amount of lysozyme used can be 0.5, 0.7, 0.8, 0.9, 1, 1.2, 1.4, 1.5 parts or any range of two values.

[0020] Optionally, the amount of pansy extract can be 0.5, 0.8, 1, 1.2, 1.5, 1.8, 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5 parts or any range of two values.

[0021] Optionally, the amount of Phellodendron bark extract can be 0.5, 0.8, 1, 1.2, 1.5, 1.8, 2, 2.2, 2.5, 2.8, 3 parts or any range of two values.

[0022] Optionally, the weight ratio of golden button flower extract to pansy extract is 1:0.8-1.5.

[0023] Optionally, the composition further comprises a polyol and water. The polyol includes at least one selected from polyethylene glycol, ethylene glycol, propylene glycol, butylene glycol, and glycerol.

[0024] Optionally, in the composition, the amount of polyol can be 10-40 parts and the amount of water can be 30-100 parts.

[0025] Optionally, the composition may also contain a solubilizer. The solubilizer may be a common cosmetic solubilizer. The amount of solubilizer in the composition may be 0.5-5 parts.

[0026] The present invention also provides a method for preparing a cosmetic composition containing an oxidoreductase, comprising adding raw materials to a reaction vessel, stirring and mixing, and then filling the mixture.

[0027] The present invention also provides the application of a cosmetic composition containing an oxidoreductase in the preparation of cosmetics, wherein the amount of the composition used in the cosmetic is 0.01-50 wt%.

[0028] The beneficial effects of this invention are as follows: This invention uses oxidoreductase, aloe vera leaf extract, fennel fruit extract, golden button flower extract, papain, lysozyme, pansy extract and phellodendron bark extract to prepare a cosmetic composition containing oxidoreductase.

[0029] Aloe vera leaf extract is rich in active ingredients such as acetylated mannan, which can form a breathable protective film on the skin surface, locking in moisture and quickly soothing symptoms of dryness and redness. Furthermore, it can inhibit the release of inflammatory factors, directly exerting an anti-inflammatory and soothing effect. Moreover, the polysaccharides and amino acids in aloe vera are a nutrient source for skin symbiotic bacteria (such as Staphylococcus epidermidis), helping to promote the colonization and reproduction of beneficial bacteria, thereby strengthening the skin's microbial barrier. To a certain extent, it can reduce the inflammation of red and swollen acne through its anti-inflammatory effect.

[0030] Anise fruit extract possesses broad-spectrum antibacterial activity, particularly exhibiting a strong inhibitory effect against Propionibacterium acnes. It disrupts bacterial cell membrane permeability, hindering their metabolism and thus directly killing acne-causing bacteria. By selectively inhibiting harmful bacteria, it creates space for beneficial bacteria, helping to restore the balance of the gut microbiota.

[0031] Golden button flower extract can significantly inhibit the activity of elastase, thus preventing the skin from sagging due to the breakdown of elastin fibers by elastase, thereby improving skin firmness and elasticity, and has excellent anti-aging and skin-firming effects.

[0032] Papain, a proteolytic enzyme, gently breaks down dead skin cells on the skin's surface, unclogging pores. By removing accumulated dead skin cells, it improves the skin's physical environment, promoting the adhesion and colonization of beneficial bacteria and preventing dysbiosis caused by dead skin buildup. It also promotes epidermal renewal and improves rough, dull skin.

[0033] Lysozyme is a natural biological enzyme that directly hydrolyzes peptidoglycan in bacterial cell walls, causing bacteria (especially Gram-positive bacteria) to rupture and die. It is part of the skin's innate immune system, acting as a biological defense to precisely eliminate invading harmful bacteria, protect symbiotic flora, and maintain the homeostasis of the skin's microecology. It can also protect sensitive skin by reducing the risk of bacterial infection and lowering the skin's inflammatory load.

[0034] Pansy extract has excellent cleansing and astringent properties. It can penetrate deep into pores, helping to dissolve oil, promote keratin metabolism, and prevent clogged pores from forming acne. It also has excellent soothing abilities, relieving skin itching and redness, and repairing damaged stratum corneum barriers. This invention uses pansy extract combined with golden button flower extract to tighten the skin by inhibiting elastase, aiding in anti-aging, and also improving skin smoothness by reducing enlarged pores.

[0035] Phellodendron bark extract has extremely strong antibacterial and anti-inflammatory effects. It can powerfully inhibit various pathogenic bacteria such as Propionibacterium acnes and Staphylococcus aureus, effectively remove acne, and quickly eliminate excessively proliferating harmful bacteria, preventing the complete collapse of the microecology.

[0036] The present invention uses anise fruit extract obtained by high-concentration ethanol extraction and ethyl acetate extraction, and a bark extract obtained by low-concentration ethanol, both of which have high efficacy in inhibiting Propionibacterium acnes and can effectively remove acne.

[0037] The tests conducted in this invention showed that when the weight ratio of anise fruit extract to Phellodendron bark extract is within the range of 1:0.2-1:5, the two extracts exhibit a synergistic effect. In other ratio ranges, they simply additively promote the inhibition of Propionibacterium acnes. Furthermore, the synergistic effect is optimal when the weight ratio of anise fruit extract to Phellodendron bark extract is within the range of 1:0.5-1:1.2, resulting in a significantly improved efficiency in inhibiting Propionibacterium acnes.

[0038] This invention utilizes a composition prepared from oxidoreductase, aloe vera leaf extract, anise fruit extract, golden button flower extract, papain, lysozyme, pansy extract, and phellodendron bark extract, which exhibits excellent anti-aging and skin-firming effects. Even after dilution, the composition retains its high efficiency in inhibiting elastase activity, thus enhancing skin firmness.

[0039] This invention combines extracts of golden button flower and pansy extract, optimizing the types of active ingredients and making the composition more efficient at inhibiting elastase, resulting in a synergistic effect in inhibiting elastase.

[0040] The composition prepared by this invention has excellent properties and can be used as a raw material to prepare cosmetics, especially in the preparation of cosmetics for acne treatment, skin tightening, and skin microecology adjustment. Attached Figure Description

[0041] Figure 1 : The average value of the inhibition zone of Propionibacterium acnes in different standards.

[0042] Figure 2 The average efficiency λ of different compositions in inhibiting elastase. Detailed Implementation

[0043] The technical solution of the present invention will be further illustrated below through specific embodiments. Those skilled in the art should understand that the embodiments described are merely illustrative of the present invention and should not be construed as limiting the invention in any way. Example 1

[0044] Anise fruit extract: Dried anise (Pimpinella anisum) fruit was first crushed and passed through a 300-mesh sieve to obtain granules. 300g of granules were taken and added to a 90wt% ethanol solution at a weight ratio of 1:20. The mixture was stirred and extracted at 40℃ and 100rpm for 2 hours. After centrifugation at 3000rpm for 30 minutes, the supernatant was filtered through a 0.2μm filter membrane. The filtrate was concentrated under reduced pressure at 55℃ for 35 minutes to remove the ethanol and obtain the concentrate. Then, ethyl acetate of 3.5 times its weight of the concentrate was added and extracted under stirring conditions at 35℃ and 70rpm. After extraction, the mixture was allowed to stand at room temperature for 1 hour. The ethyl acetate phase was then concentrated under reduced pressure at 40℃ for 50 minutes to remove the ethyl acetate and obtain the anise fruit extract.

[0045] Phellodendron amurense bark extract: Dried Phellodendron amurense bark was first crushed and passed through a 300-mesh sieve to obtain granules. 300g of granules were taken and added to a 20wt% ethanol solution at a weight ratio of 1:30. The mixture was stirred and extracted at 45℃ and 100rpm for 5 hours, then centrifuged at 3000rpm for 30 minutes. The supernatant was filtered through a 0.2μm filter membrane. The filtrate was concentrated under reduced pressure at 50℃ for 40 minutes to remove the ethanol and obtain the concentrate. Then, the concentrate was freeze-dried at -50℃ for 15 hours to obtain the Phellodendron amurense bark extract. Example 2

[0046] The extract prepared in Example 1 was tested for its acne-removing effect. The inhibition rate of the extract against Propionibacterium acnes was tested to verify its acne-removing efficacy. Test samples were prepared according to the following composition: Standard 1: Anise fruit extract, diluted with 0.5wt% Tween-60 aqueous solution to a concentration of 2.5wt%.

[0047] Standard 2: Phellodendron bark extract, diluted with 0.5wt% Tween-60 aqueous solution to a concentration of 2.5wt%.

[0048] Standard 3: Anise fruit extract and Phellodendron bark extract were diluted to a total concentration of 1 wt% with 0.5 wt% Tween-60 aqueous solution at a weight ratio of 1:0.05.

[0049] Standard 4: Anise fruit extract and Phellodendron bark extract were diluted to a total concentration of 1 wt% with 0.5 wt% Tween-60 aqueous solution at a weight ratio of 1:0.2.

[0050] Standard 5: Anise fruit extract and Phellodendron bark extract were diluted to a total concentration of 1 wt% with 0.5 wt% Tween-60 aqueous solution at a weight ratio of 1:0.5.

[0051] Standard 6: Anise fruit extract and Phellodendron bark extract were diluted to a total concentration of 1 wt% with 0.5 wt% Tween-60 aqueous solution at a weight ratio of 1:0.8.

[0052] Standard 7: Anise fruit extract and Phellodendron bark extract were diluted to a total concentration of 1 wt% with 0.5 wt% Tween-60 aqueous solution at a weight ratio of 1:1.

[0053] Standard 8: Anise fruit extract and Phellodendron bark extract were diluted to a total concentration of 1 wt% with 0.5 wt% Tween-60 aqueous solution at a weight ratio of 1:1.2.

[0054] Standard 9: Anise fruit extract and Phellodendron bark extract were diluted with 0.5wt% Tween-60 aqueous solution at a weight ratio of 1:5 to a total concentration of 1wt%.

[0055] Standard 10: Anise fruit extract and Phellodendron bark extract were diluted to a total concentration of 1 wt% with 0.5 wt% Tween-60 aqueous solution at a weight ratio of 1:10.

[0056] The test method is as follows: Prepare a bacterial concentration of 1.0 × 10⁻⁶. 8 CFU / mL bacterial suspension (Propionibacterium acnes, bacterial number ATCC6919, logarithmic growth phase) was added to a sterile petri dish with melted agar medium. The mixture was allowed to solidify, and the bacterial suspension was evenly spread onto the agar surface using a cotton swab. This was repeated four times. The mixture was then allowed to stand at room temperature for 10 minutes to obtain the test carrier. 7.5 μL of standard sample was added to the surface of sterile filter paper and then placed on the test carrier. The mixture was incubated at 37°C with 5% CO2 for 20 hours. The inhibition zone was then tested. Each group was tested in triplicate. The results are shown in Table 1 and [Table data missing]. Figure 1 .

[0057] Table 1: Inhibition zones of Propionibacterium acnes

[0058] Combining Table 1 and Figure 1 Tests show that the anise fruit extract obtained by high-concentration ethanol extraction and ethyl acetate extraction, and the Phellodendron bark extract obtained by low-concentration ethanol, both have high inhibitory effects on Propionibacterium acnes and can effectively remove acne.

[0059] In Table 1 above, samples 1 and 2 were tested for inhibition rates using anise fruit extract and phellodendron bark extract alone. Samples 4-9 showed better inhibition rates than samples 1-2 alone. This indicates that within a weight ratio of 1:0.2 to 1:5, the combination of anise fruit extract and phellodendron bark extract has a synergistic effect. In other ratios, they simply additively promote the inhibition of Propionibacterium acnes. Furthermore, samples 5-8 showed the highest inhibition rate. This demonstrates that within a weight ratio of 1:0.5 to 1:1.2, the combination of anise fruit extract and phellodendron bark extract exhibits the best synergistic effect, resulting in a significantly improved inhibition rate of Propionibacterium acnes. Example 3

[0060] Spilanthes acmella flower extract: Dried Spilanthes acmella flowers were first crushed and passed through a 300-mesh sieve to obtain granules. 200g of granules were taken and added to an 85wt% ethanol solution at a weight ratio of 1:20. The mixture was stirred and extracted at 45℃ and 120rpm for 3 hours, then centrifuged at 3000rpm for 35 minutes. The supernatant was filtered through a 0.2μm filter membrane, and the filtrate was concentrated under reduced pressure at 55℃ for 32 minutes to remove the ethanol and obtain the concentrate. Then, ethyl acetate was added at 4 times the weight of the concentrate and extracted under stirring conditions at 35℃ and 80rpm. After extraction, the mixture was allowed to stand at room temperature for 1.2 hours. The ethyl acetate phase was then concentrated under reduced pressure at 40℃ for 45 minutes to remove the ethyl acetate and obtain the Spilanthes acmella flower extract.

[0061] Pansy extract: The dried aerial parts of pansy (VIOLA TRICOLOR) were first crushed and passed through a 300-mesh sieve to obtain granules. 250g of granules were taken and added to a 22.5wt% ethanol solution at a weight ratio of 1:28. The mixture was stirred and extracted at 47℃ and 100rpm for 5.5 hours. After centrifugation at 3000rpm for 35 minutes, the supernatant was filtered through a 0.2μm filter membrane. The filtrate was concentrated under reduced pressure at 50℃ for 50 minutes to remove ethanol and obtain the concentrate. Then, the concentrate was freeze-dried at -52℃ for 14 hours to obtain pansy extract.

[0062] Oxidoreductase: Catalase, enzyme activity 200,000 U / g, Zhengzhou Wanbo Chemical Products Co., Ltd. Papain: enzyme activity 100,000 U / g, Zhengzhou Maida Biotechnology Co., Ltd. Lysozyme: enzyme activity 20,000 U / mg, Shandong Jishuo Biotechnology Co., Ltd. Solubilizer: Tween-60.

[0063] Aloe vera leaf extract: Take 50g of fleshy part of Aloe vera leaf, add 40 times its weight of water, stir and extract at 50℃ and 80rpm for 3 hours, then centrifuge at 3000rpm, collect the clear liquid, concentrate and freeze dry at -50℃ for 12 hours to obtain Aloe vera leaf extract.

[0064] Prepare the raw materials for the composition according to the raw material composition in Table 2.

[0065] Table 2: Raw material composition of the composition

[0066] The preparation method of the above composition is as follows: water is added to the reaction vessel, heated to 45°C, stirred at 120 rpm, polyol and solubilizer are added, stirred for 5 minutes, then the remaining raw materials are added, and stirring is continued for 35 minutes while maintaining the temperature and speed. Then the mixture is filtered, cooled to room temperature, and filled into containers.

[0067] Example 4 The anti-aging and skin-firming effects of the composition prepared in Example 3 were tested, and the efficiency of the composition in inhibiting elastase was verified.

[0068] The testing method is as follows: Reagents: PBS, test sample (composition diluted to 2.5 wt% with PBS), substrate solution (2 mM N-succinyl-alanine-alanine-p-nitroaniline, prepared with PBS), elastase solution (porcine pancreatic elastase, concentration 0.2 U / mL, prepared with PBS).

[0069] Group setup: Model group: 25 μL elastase solution + 50 μL substrate solution + 25 μL PBS. Control group: 50 μL substrate solution + 50 μL PBS. Sample group: 25 μL test sample + 25 μL elastase solution + 50 μL substrate solution.

[0070] Based on the above group settings, different groups of test substances were prepared and incubated at room temperature in the dark for 15 minutes. The absorbance (OD) was measured at 420 nm using an ELISA reader, and the tests were performed in triplicate. The efficiency of inhibiting elastase was calculated using the following formula: λ = 100% × [1 - (OD1 - OD0) / (OD2 - OD0)]; where OD0 represents the absorbance of the blank group, OD1 represents the absorbance of the sample group, and OD2 represents the absorbance of the model group. The results are shown in Table 3 and... Figure 2 .

[0071] Table 3: Efficiency λ in inhibiting elastase

[0072] Combined with Table 3 and Figure 2 Tests show that the composition prepared by this invention using oxidoreductase, aloe vera leaf extract, anise fruit extract, golden button flower extract, papain, lysozyme, pansy extract, and phellodendron bark extract has excellent anti-aging and skin-firming effects. Even after dilution, the composition still effectively inhibits elastase activity, thus enhancing skin firmness.

[0073] Compared to composition A, which uses only one of the extracts of *Gynostemma pentaphyllum* and *Viola tricolor*, compositions C and D show a certain degree of decreased efficacy in inhibiting elastase. This indicates that combining the two optimizes the types of active ingredients, resulting in higher elastase inhibition efficiency and a synergistic effect. Composition E lacks multiple active ingredients, leading to low elastase inhibition and poor application results.

[0074] This specific embodiment is merely an explanation of the present invention and is not intended to limit the invention. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they are within the scope of the claims of the present invention.

Claims

1. A cosmetic composition containing an oxidoreductase, characterized in that, The raw materials, by weight, are as follows: 1.5-3.5 parts oxidoreductase, 0.3-2.5 parts aloe vera leaf extract, 0.5-3 parts anise fruit extract, 0.6-4 parts golden button flower extract, 0.8-2.5 parts papain, 0.5-1.8 parts lysozyme, 0.5-3.5 parts pansy extract, and 0.5-3 parts Amur cork tree bark extract.

2. The cosmetic composition containing an oxidoreductase according to claim 1, characterized in that, Oxidoreductases include at least one of lactate dehydrogenase, succinate dehydrogenase, cytochrome oxidase, catalase, and peroxidase.

3. The cosmetic composition containing an oxidoreductase according to claim 1, characterized in that, Both the fennel fruit extract and the golden button flower extract were first pulverized, then extracted with 80-95wt% ethanol, centrifuged, filtered, concentrated to remove the ethanol, and then extracted with ethyl acetate and concentrated to obtain the final product.

4. A cosmetic composition containing an oxidoreductase according to claim 1, characterized in that, Both the bark extract of Phellodendron amurense and the extract of Viola tricolor were obtained by first pulverizing the bark, then extracting with 15-30 wt% ethanol, followed by centrifugation, filtration, concentration and drying.

5. A cosmetic composition containing an oxidoreductase according to claim 1, characterized in that, The composition also contains polyols and water.

6. A cosmetic composition containing an oxidoreductase according to claim 5, characterized in that, Polyols include at least one of polyethylene glycol, ethylene glycol, propylene glycol, butylene glycol, and glycerol.

7. A cosmetic composition containing an oxidoreductase according to claim 1, characterized in that, The weight ratio of golden button flower extract to pansy extract is 1:0.8-1.

5.

8. A cosmetic composition containing an oxidoreductase according to claim 5, characterized in that, The composition also contains a solubilizer.

9. A method for preparing a cosmetic composition containing an oxidoreductase according to any one of claims 1-8, characterized in that, This involves adding raw materials to a reaction vessel, stirring and mixing them, and then filling the container to obtain the final product.

10. The use of a cosmetic composition containing an oxidoreductase according to any one of claims 1-8 or a cosmetic composition containing an oxidoreductase prepared by the preparation method according to claim 9 in the preparation of cosmetics, characterized in that, The composition is used in cosmetics in an amount of 0.01-50 wt%.

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