A slightly acidic sebum control and repair composition for male skin and its application

Abstract

This invention discloses a micro-acid oil-controlling and repairing composition specifically designed for men to regulate sebaceous glands and its application, belonging to the field of daily cosmetics technology. The micro-acid oil-controlling and repairing composition comprises active ingredients and excipients, wherein the active ingredients are composed of the following raw materials: sodium DNA, chlorogenic acid, succinic acid, ammonium glycyrrhizate, and hydroxyethylpiperazine ethanesulfonic acid. The micro-acid oil-controlling and repairing composition of this invention does not contain any ingredients restricted for use in cosmetics, is gentle and non-irritating, and through multi-target synergistic action, can significantly inhibit androgen receptor expression, 5α-reductase activity, and the proliferation of Propionibacterium acnes, fundamentally blocking the excessive sebum secretion pathway, simultaneously achieving multiple effects of oil control, acne removal, anti-inflammatory soothing, and barrier repair. It can be widely used in the preparation of various oil-controlling and acne-removing skincare products, especially suitable for the skincare needs of men with oily skin.

Description

Technical Field

[0001] This invention belongs to the technical field of daily cosmetics, specifically relating to a mildly acidic oil-controlling and repairing composition for men that regulates sebaceous glands and its application. Background Technology

[0002] Oily skin is a normal physiological process caused by the sebaceous glands secreting sebum. Sebum acts like a "natural protective film" for the skin, locking in moisture, resisting external stimuli, and maintaining healthy skin. However, when affected by factors such as hormonal fluctuations, genetic factors, high temperatures, and stress, the sebaceous glands can become overworked, secreting excessive oil. This not only makes the skin appear oily but also easily clogs pores, leading to problems such as blackheads and acne.

[0003] Current research often uses hydroxy acids to achieve oil control effects on the skin. However, these ingredients are restricted in the cosmetics industry and can irritate and damage the skin at higher concentrations. The probability of adverse reactions increases with increasing concentration and longer application time. For example, patent 202210373916.6 uses an acid complex: salicylic acid, lactobionic acid, gluconolactone, glycolic acid, and mandelic acid. All of these ingredients are restricted in cosmetics and are subject to strict industry safety standards. The acid complex accounts for 75-98% of the product; if calculated according to the upper limit for added amounts in cosmetic compositions, the actual acid concentration may be close to the safety threshold. Patch testing has confirmed that 7 people developed mild erythema after 48 hours with a sample containing a high proportion of acids (serum B), indicating a certain risk of skin irritation. It is difficult to simultaneously achieve both gentleness and oil control in this product. In addition, the patented acid compound uses a simple mixing process. Since different acids have large differences in polarity, simple mixing can easily lead to uneven dispersion of components, which may cause local irritation and affect the oil control effect of the product.

[0004] The reason why oily skin is more pronounced in men is primarily driven by the specific interaction mechanism between androgens and androgen receptors on sebaceous glands. Men have significantly higher levels of androgens, such as testosterone, than women. These androgens bind to androgen receptors on the surface of sebaceous gland cells, activating the receptor's signaling pathways. This further triggers the expression of lipid synthesis-related genes within the sebaceous gland cells, accelerating sebum synthesis. Simultaneously, 5α-reductase in the skin converts testosterone into the more active dihydrotestosterone (DHT), further promoting sebum secretion and leading to acne. Existing patent 202510934817.4 uses a synergistic combination of mandelic acid, salicylic acid, and succinic acid to control oil production by inhibiting 5α-reductase expression and the growth of Propionibacterium acnes. Patent 202510390540.3 uses extracts from Norway spruce leaves, soapberry fruit, schisandra chinensis, soybean seeds, jujube bark, and phellodendron bark to achieve oil control for men by inhibiting 5α-reductase activity. None of the above patents fundamentally address the core target of male oil production—the androgen receptor—and therefore suffer from poor oil control effects. Summary of the Invention

[0005] To address the shortcomings of existing technologies, the present invention aims to provide a mildly acidic oil-controlling and repairing composition. This composition is free of restricted cosmetic ingredients, is gentle and safe, and achieves synergistic effects on multiple targets through the scientific compounding of multiple components. It blocks excessive sebum secretion from the root cause and simultaneously achieves multiple effects such as antibacterial and acne-removing, anti-inflammatory and soothing, and barrier repair.

[0006] To achieve the above objectives, the present invention discloses the following technical solutions: In a first aspect, the present invention provides a slightly acidic oil-controlling and repairing composition, wherein the composition contains active ingredients and excipients; The active ingredient, by weight, is composed of the following raw materials: 8-11 parts sodium DNA, 4-6 parts chlorogenic acid, 8-12 parts succinic acid, 13-17 parts ammonium glycyrrhizate, and 81-98 parts hydroxyethylpiperazine ethane sulfonic acid.

[0007] Preferably, the excipient is at least one selected from humectants, thickeners, antibacterial agents, and solvents.

[0008] Preferably, the humectant is at least one selected from glycerin, 1,2-hexanediol, and butylene glycol.

[0009] Preferably, the thickener is at least one of xanthan gum, carbomer, carrageenan, and gellan gum.

[0010] Preferably, the antibacterial agent is at least one selected from p-hydroxyacetophenone, phenoxyethanol, 1,2-hexanediol, and butanediol.

[0011] Preferably, the solvent is deionized water.

[0012] Secondly, the present invention provides a method for preparing a slightly acidic oil-controlling and repairing composition, the preparation method comprising the following steps: Step 1: Dissolve sodium DNA in deionized water and stir to dissolve, thus obtaining a sodium DNA solution; Step 2: Add chlorogenic acid and ammonium glycyrrhizate to glycerol and stir at 40-80℃ and 150-250r / min for 10-60min to obtain mixed solution A; Step 3: Dissolve hydroxyethylpiperazine ethanesulfonic acid and succinic acid in deionized water, stir to dissolve, and adjust the pH to 4.0-6.0 using sodium hydroxide to obtain mixed solution B; Step 4: Slowly add sodium DNA solution to mixed solution B, then slowly add mixed solution A. Homogenize using a high-pressure homogenizer at a pressure of 10-150 MPa and a temperature of 20-50℃ for 5-20 minutes. Finally, add 1,2-hexanediol, carrageenan, and deionized water, stir evenly, and sterilize at 100℃ for 0.5-2 hours to obtain the micro-acid oil-controlling repair composition.

[0013] Preferably, the mass ratio of sodium DNA, chlorogenic acid, succinic acid, ammonium glycyrrhizate and hydroxyethylpiperazine ethanesulfonic acid is (8-11):(4-6):(8-12):(13-17)(48-52).

[0014] Preferably, the total amount of sodium DNA, chlorogenic acid, succinic acid, ammonium glycyrrhizate and hydroxyethylpiperazine ethanesulfonic acid added to the slightly acidic oil-controlling and repairing composition is 5-10 wt%.

[0015] Thirdly, the present invention provides the application of the composition described in the first aspect in the preparation of skin care products and hair care products with oil-controlling effects.

[0016] Preferably, the skin care product is a serum, lotion, cream, or mask; the hair care product is a shampoo, styling spray, styling water, styling cream, or styling gel.

[0017] In this invention, sodium DNA is derived from low-molecular-weight nucleic acid active ingredients from salmon testes. It can inhibit excessive inflammatory responses and inflammatory cell infiltration, promote cell proliferation and migration, collagen deposition and angiogenesis, accelerate skin wound healing, repair the skin barrier through anti-inflammatory effects, help improve rosacea-related telangiectasia and redness and stinging of acne-prone skin, help maintain skin barrier homeostasis, inhibit collagen degradation, promote collagen synthesis, improve aging problems such as wrinkles, roughness and enlarged pores in oily skin, reduce excessive proliferation of fibroblasts and abnormal collagen deposition, prevent hypertrophic scars after acne healing, and inhibit tyrosinase activity, reduce melanin production, and improve post-inflammatory hyperpigmentation and uneven skin tone associated with oiliness.

[0018] Chlorogenic acid, the core active ingredient of this invention, covers all target pathways for oil control. It competitively antagonizes androgen receptors, reduces receptor expression, blocks androgen-mediated sebum secretion signals, and strongly inhibits 5α-reductase activity to reduce the production of highly active dihydrotestosterone (DHT), thus inhibiting sebaceous gland hyperplasia and excessive sebum secretion. It also inhibits the AKT / mTOR / SREBP signaling pathway, specifically blocking ACC-mediated de novo lipid synthesis, reducing sebum production from the core pathway and achieving targeted oil control at the root. Simultaneously, it can effectively scavenge superoxide anions. It contains various free radicals such as hydroxyl radicals and hydrogen peroxide, which significantly inhibit the peroxidation of oil, block the excessive sebum secretion induced by free radicals, maintain the balance of free radicals in the skin, and inhibit the release of various pro-inflammatory factors and inflammatory mediators such as TNF-α and IL-6, thus blocking the vicious cycle of "excessive sebum-inflammation-more sebum secretion" and improving the redness and swelling inflammatory response induced by oiliness. At the same time, it can significantly inhibit the growth of various harmful microorganisms such as Propionibacterium acnes and Staphylococcus aureus, regulate the skin microecological balance, and reduce acne and pimple problems induced by oiliness.

[0019] Succinic acid is the core microacid component of the oil-controlling system of this invention. As a small-molecule dicarboxylic acid naturally found in the bodies of animals and plants and in the metabolic products of microorganisms, it has both formulation regulation and skin care value. It has excellent pH buffering and regulation capabilities, can maintain the skin's microacidic environment for a long time, ensure the activity of enzymes related to lipid metabolism in the stratum corneum, reduce sebum metabolism disorders and barrier function damage induced by pH abnormalities, and help maintain the skin's pH homeostasis. At the same time, it can inhibit the growth and skin colonization of Propionibacterium acnes, block the inflammatory response induced by this bacterium, reduce acne-related redness and swelling, and simultaneously help regulate the balance of skin inflammation and microbial balance. As a key endogenous intermediate product of the human tricarboxylic acid cycle, it can also enhance the activity of mitochondria in skin cells, accelerate the regeneration of damaged tissue, cell renewal and wound repair, help repair the skin barrier and regenerate sebaceous glands, improve skin pigmentation, regulate the overall skin homeostasis, and help alleviate the skin roughness and barrier damage problems associated with oiliness.

[0020] Ammonium glycyrrhizate is the core soothing and repairing ingredient in the oil-controlling system of this invention. It is an ammonium salt derivative of glycyrrhizic acid, extracted from licorice, a plant with both medicinal and edible properties. Compared to unmodified glycyrrhizic acid, it achieves a dual breakthrough in water solubility and activity, retaining the natural gentle repairing power of licorice while being suitable for cosmetic formulations. It has a good inhibitory effect on acute, chronic, and immune-mediated inflammation, reducing redness and itching caused by inflammation. It can directly antagonize various inflammatory mediators such as histamine and prostaglandin E2, while also promoting the production of anti-inflammatory factors and inhibiting TNF-α. It releases multiple pro-inflammatory factors such as IL-8, effectively blocking the vicious cycle between oil production and inflammation. It is highly suitable for the soothing needs of oily, acne-prone, and sensitive skin. At the same time, it has broad-spectrum antioxidant capabilities, which can effectively remove free radicals such as DPPH and ABTS+, reduce oil peroxidation and oxidative stress damage, help maintain the skin's free radical balance, improve the dull skin tone problem associated with oil production, and reduce the continuous damage of inflammation to the skin barrier by inhibiting excessive inflammatory response. It also helps maintain the skin barrier homeostasis and water-oil homeostasis, and improves the barrier damage problems common in oily skin.

[0021] Hydroxyethylpiperazine ethane sulfonic acid is the core buffering and keratin conditioning ingredient in the oil-controlling system of this invention. As an amphoteric buffer, it combines formula stability and skincare efficacy with its excellent physicochemical properties. It can gently exfoliate the aged keratinocytes on the skin surface by loosening the connections between keratinocytes, reducing the accumulation of dead keratinocytes that cause clogged pores and rough skin, unclogging sebaceous gland ducts, and improving the problem of blocked oil excretion. At the same time, it can gently remove keratinocytes containing melanin from the epidermis, improve uneven skin tone and dullness caused by oiliness, and even out and brighten the skin tone. Its buffering range is wide and its stability is strong. It is not significantly affected by temperature changes and can maintain the slightly acidic environment of the formula system and the skin for a long time, ensuring the activity of lipid metabolism enzymes in the stratum corneum, stabilizing the skin barrier function, reducing sebum metabolism disorders induced by pH abnormalities, and protecting the active ingredients and skin from ultraviolet and visible light radiation. It adsorbs and partially reflects harmful radiation, reducing abnormal sebum secretion and skin aging problems induced by photodamage.

[0022] This invention has discovered that the compound system formed by sodium DNA, chlorogenic acid, succinic acid, ammonium glycyrrhizate, and hydroxyethylpiperazine ethanesulfonic acid achieves oil control and acne removal effects by targeting multiple points, including inhibiting androgen receptor expression, inhibiting 5α-reductase activity, and inhibiting the growth of Propionibacterium acnes.

[0023] The beneficial effects of this invention are: 1. The ammonium glycyrrhizate, chlorogenic acid, succinic acid, and sodium DNA used in this invention are all naturally sourced ingredients, and none of the raw materials are restricted ingredients in cosmetics. They are gentler and safer to use and will not cause potential skin irritation risks.

[0024] 2. This invention employs multiple ingredients to synergistically enhance oil control by targeting multiple points, including inhibiting androgen receptor expression, inhibiting 5α-reductase activity, and inhibiting the growth of Propionibacterium acnes, resulting in a more comprehensive oil control effect. Detailed Implementation

[0025] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0026] The technical solution of the present invention will be further described in detail below with reference to specific embodiments. It should be understood that the following embodiments are only used to explain the present invention and are not intended to limit the present invention.

[0027] I. In this invention: Sodium DNA, chlorogenic acid, succinic acid, ammonium glycyrrhizate, and hydroxyethylpiperazine ethane sulfonic acid are all commercially available raw materials.

[0028] II. Mildly Acidic Oil-Controlling and Repairing Composition

[0029] 1. Example In this invention, sodium DNA, chlorogenic acid, succinic acid, ammonium glycyrrhizate and hydroxyethylpiperazine ethanesulfonic acid are used as the active ingredients in the micro-acid oil-controlling and repairing composition. The mass ratio of each raw material in active ingredients 1-3 is shown in Table 1. The composition of the micro-acid oil-controlling and repairing composition in Examples 1-3 is shown in Table 2.

[0030] Table 1. Raw materials and their mass fractions of the active ingredients Raw material name Active ingredient 1 Active ingredient 2 Active ingredient 3 Sodium DNA 8 10 11 chlorogenic acid 4 5 6 Succinic acid 8 10 12 ammonium glycyrrhizate 13 15 17 Hydroxyethylpiperazine ethanesulfonic acid 48 50 52 Table 2. Components and mass percentages of the composition (unit: %) Raw material name Example 1 Example 2 Example 3 Active ingredient 1 7 / / Active ingredient 2 / 7 / Active ingredient 3 / / 7 Carrageenan 0.1 0.1 0.1 1,2-Hexanediol 2 2 2 glycerin 20 20 20 Deionized water Add to 100 Add to 100 Add to 100 Note: " / " in the table indicates no addition.

[0031] The preparation method of the slightly acidic oil-controlling and repairing composition is as follows: Step 1: Dissolve sodium DNA in 1 / 3 of the volume of deionized water, stir to dissolve, and obtain sodium DNA solution; Step 2: Add chlorogenic acid and ammonium glycyrrhizate to glycerol and stir at 60℃ and 200r / min for 30min to obtain mixed solution A; Step 3: Dissolve hydroxyethylpiperazine ethanesulfonic acid and succinic acid in 1 / 3 of the volume of deionized water, stir to dissolve, and adjust the pH to 5.0 using sodium hydroxide to obtain mixed solution B; Step 4: Slowly add sodium DNA solution to mixed solution B, then slowly add mixed solution A to mixed solution B. Homogenize using a high-pressure homogenizer at 100 MPa and 20°C for 10 min. Finally, add 1,2-hexanediol, carrageenan, and the remaining deionized water. Stir well and sterilize at 100°C for 1 h to obtain the micro-acid oil-controlling repair composition.

[0032] 2. Comparative Example The active ingredients 4-9 are obtained by adjusting the active ingredient 2, as shown in Table 3; the composition of the compositions in Comparative Examples 1-6 is shown in Table 4, and the preparation method is the same as that in the examples.

[0033] Table 3. Raw materials and their mass fractions of the active ingredients Raw material name Active ingredient 4 Active ingredient 5 Active ingredient 6 Active ingredient 7 Active ingredient 8 Active ingredient 9 Sodium DNA / 10 10 10 10 5 chlorogenic acid 5 / 5 5 5 10 Succinic acid 10 10 / 10 10 10 ammonium glycyrrhizate 15 15 15 / 15 15 Hydroxyethylpiperazine ethanesulfonic acid 50 50 50 50 / 50 Note: " / " in the table indicates no addition.

[0034] Table 4. Components of the composition and their mass percentages (unit: %) Raw material name Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Active ingredient 4 7 / / / / Active ingredient 5 / 7 / / / Active ingredient 6 / / 7 / / Active ingredient 7 / / / 7 / Active ingredient 8 / / / / 7 Active ingredient 9 / / / / / 7 Carrageenan 0.1 0.1 0.1 0.1 0.1 0.1 1,2-Hexanediol 2 2 2 2 2 2 glycerin 20 20 20 20 20 20 Deionized water Add to 100 Add to 100 Add to 100 Add to 100 Add to 100 Add to 100 Note: " / " in the table indicates no addition.

[0035] III. Performance Testing 1. Androgen receptor expression experiment 1.1 Experimental Principle and Objective Excessive sebum secretion is a core factor leading to oily skin, enlarged pores, and acne. In the physiological activities of sebaceous gland cells, the androgen and its receptor (AR) signaling pathway plays a decisive role. After androgens such as dihydrotestosterone (DHT) enter sebaceous gland cells, they bind to AR in the cytoplasm, activating AR and translocating it to the cell nucleus. This, in turn, initiates the expression of downstream lipid synthesis-related genes, resulting in excessive sebum secretion.

[0036] This experiment used human primary SZ95 sebaceous gland cells as the research object. By analyzing the expression level of AR protein in the cells after the sample was treated, the oil-controlling effect of the sample was evaluated.

[0037] 1.2 Experimental Scheme 1.2.1 Sample The compositions prepared in Examples 1-3 and Comparative Examples 1-6. During the experiments, each group of samples needed to be diluted with culture medium to the corresponding concentration of the test sample solution.

[0038] 1.2.2 Safe working concentration Based on keratinocyte cytotoxicity assays, the MTT assay was used to detect the toxic effects of different concentrations of samples on SZ95 sebaceous gland cells, and the safe working concentration range of the samples was screened for subsequent efficacy evaluation experiments. Seven concentration gradients were selected for MTT assays, and then sample concentrations with cell viability ≥90% were selected for subsequent efficacy testing. Finally, at ≤0.05wt%, cell viability was ≥90% in all groups, and 0.05wt% sample concentration was determined as the safe concentration for subsequent efficacy testing.

[0039] 1.2.2 Experimental Grouping Table 5 Experimental Groups

[0040] 1.2.3 Experimental Procedure Cell seeding: Cells were seeded in 24-well plates at a density of 0.5 × 10⁶ cells / well. 5 / mL, incubate for 24h.

[0041] Add 1 mL of sample to each culture medium according to the above grouping method, and incubate for 48 h.

[0042] AR protein content detection: Cells were removed, fixed with 4% paraformaldehyde, and detected by immunofluorescence.

[0043] Calculation formula: Relative expression level of AR protein (%) = Sample group / Negative control group × 100% 1.2.4 Experimental Results Table 6. Relative expression levels of AR proteins Group AR protein relative expression level / % negative control group 100.00 Model group 285.32 Positive control group 122.47 Example 1 156.53 Example 2 142.78 Example 3 153.63 Comparative Example 1 221.35 Comparative Example 2 268.49 Comparative Example 3 215.67 Comparative Example 4 208.92 Comparative Example 5 230.16 Comparative Example 6 171.49 1.2.5 Results Analysis Compared with the negative control group, after induction with 10 μM DHT, the relative expression level of AR protein in SZ95 sebaceous gland cells in the model group was significantly increased to 285.32%, indicating that DHT can significantly upregulate androgen receptor expression, and a cell model of hypersedematous sebum secretion was successfully constructed.

[0044] Compared with the model group, Examples 1-3 of the present invention can downregulate the high expression of DHT-induced AR protein, and the inhibitory effect on AR expression is close to that of the positive control finasteride, proving that the composition of the present invention can effectively block the core target of androgen action and inhibit the androgen-mediated sebum secretion signaling pathway from the root.

[0045] Compared with Example 2, the relative expression levels of AR protein in Comparative Examples 1-6 were significantly increased, indicating that the complex system formed by sodium DNA, chlorogenic acid, succinic acid, ammonium glycyrrhizate and hydroxyethylpiperazine ethanesulfonic acid has a synergistic effect. The absence of any component or the ratio being outside the specific range will lead to a significant decrease in the inhibitory effect of the composition on androgen receptors.

[0046] 2. 5α-Reductase Inhibition Experiment 2.1 Experimental Objectives and Principles Sebum secretion is closely related to sebaceous gland function, which in turn is closely related to androgen regulation. 5α-reductase is a membrane protease located on microsomes and the cell nucleus. Using the reduced coenzyme NADPH as a hydrogen donor, it catalyzes the conversion of testosterone to dihydrotestosterone (DHT), which induces excessive sebum secretion from the sebaceous glands. Inhibiting 5α-reductase activity reduces DHT production and lowers sebaceous gland lipid secretion levels.

[0047] 2.2 Sample Solution The compositions in the examples and comparative examples were diluted with physiological saline to prepare sample solutions with a concentration of 0.5 wt%.

[0048] 2.3 Experimental Methods Sample pretreatment: 20 µL each of the sample solution and the negative control (physiological saline) were added to the enzyme reaction system for catalysis. The absorbance was measured at 340 nm using a UV spectrophotometer. The average absorbance was used to calculate the enzyme activity. Finally, the 5α-reductase inhibition rate was calculated.

[0049] 2.4 Experimental Results Table 7 5α-Reductase Inhibition Rate Group Inhibition rate / % Example 1 36.66 Example 2 38.62 Example 3 35.18 Comparative Example 1 18.72 Comparative Example 2 12.35 Comparative Example 3 20.14 Comparative Example 4 22.58 Comparative Example 5 19.43 Comparative Example 6 27.11 2.5 Results Analysis The compositions of Examples 1-3 of this invention all have excellent inhibitory effects on 5α-reductase, proving that the compositions of this invention can effectively inhibit the activity of 5α-reductase, reduce the conversion of testosterone to highly active dihydrotestosterone (DHT), thereby blocking DHT-mediated sebaceous gland hyperplasia and excessive sebum secretion, and achieving oil control effect from the core pathway.

[0050] 3. Experiment on inhibiting Propionibacterium acnes 3.1 Experimental Objectives and Principles Based on the fact that *Propionibacterium acnes* is a normal component of the hair follicle flora, and that *Propionibacterium acnes* proliferates during the inflammatory process of acne, this study examines the antibacterial effect of *Propionibacterium acnes* in samples to evaluate their acne-reducing efficacy. *Propionibacterium acnes*, a component of the normal hair follicle flora, uses sebum as its active ingredient, partially converting it into free fatty acids. *Propionibacterium acnes* can also activate catalysis, producing at least two chemokines that promote inflammation. Therefore, inhibiting *Propionibacterium acnes* can achieve an acne-reducing effect. The acne-reducing efficacy of the test samples is evaluated by detecting their antibacterial effect on *Propionibacterium acnes*.

[0051] 3.2 Experimental Methods 3.2.1 Sample The compositions in the examples and comparative examples were diluted with PBS buffer at pH 7.2 to prepare sample solutions with a concentration of 0.5 wt%.

[0052] 3.2.2 Method Experimental bacterial species and suspension range: Propionibacterium acnes (ATCC11827), 1×10 4 ~9×10 4 CFU / mL. The quantitative suspension inhibition test was performed according to QB / T 2738-2023 "Evaluation Methods for Antibacterial and Bacteriostatic Effects of Daily Chemical Products". Quantitative suspension test: Add sample, react for 5 min, and repeat the test three times. Prepare approximately 1×10⁻⁶ CFU / mL from fresh slant culture. 4 ~9×10 4 Set aside the CFU / mL bacterial suspension. Take 0.4 mL of the bacterial suspension and add it to 20 mL of the sample solution. Mix quickly and start timing immediately. Inoculate two dishes with the sample mixture and pour in the culture medium. Repeat the experiment three times. Place the dishes in a 36℃ biochemical incubator and incubate for 48 hours. Observe the results, count the viable bacteria, and calculate the average inhibition rate.

[0053] 3.2.5 Calculation formula: X = (AB) / A × 100% In the formula: X—inhibition rate (%); A—average recovered colonies in the control group; B—average recovered colonies in the experimental group.

[0054] 3.3 Experimental Results Table 8 Antibacterial rate Group Antibacterial rate / % Example 1 87.37 Example 2 89.20 Example 3 90.45 Comparative Example 1 90.38 Comparative Example 2 71.72 Comparative Example 3 75.49 Comparative Example 4 73.16 Comparative Example 5 91.33 Comparative Example 6 92.25 3.4 Results Analysis The compositions of Examples 1-3 of this invention all have a strong inhibitory effect on Propionibacterium acnes, proving that the compositions of this invention can effectively inhibit the growth and reproduction of Propionibacterium acnes, thereby blocking the inflammatory response and acne formation induced by the proliferation of Propionibacterium acnes after excessive sebum secretion, and achieving excellent acne removal effect.

[0055] While specific embodiments of the present invention have been described above, those skilled in the art should understand that these are merely illustrative examples, and the scope of protection of the present invention is defined by the appended claims. Those skilled in the art can make various changes or modifications to these embodiments without departing from the principles and essence of the present invention, but all such changes and modifications fall within the scope of protection of the present invention.

Claims

1. A slightly acidic oil-controlling and repairing composition, characterized in that, The composition contains active ingredients and excipients; The active ingredient, by weight, is composed of the following raw materials: 8-11 parts sodium DNA, 4-6 parts chlorogenic acid, 8-12 parts succinic acid, 13-17 parts ammonium glycyrrhizate, and 48-52 parts hydroxyethylpiperazine ethane sulfonic acid. The excipients are at least one of humectants, thickeners, antibacterial agents, and solvents.

2. The composition according to claim 1, characterized in that, The humectant is at least one of glycerin, 1,2-hexanediol, and butylene glycol.

3. The composition according to claim 1, characterized in that, The thickener is at least one of xanthan gum, carbomer, carrageenan, and gellan gum.

4. The composition according to claim 1, characterized in that, The antibacterial agent is at least one of p-hydroxyacetophenone, phenoxyethanol, 1,2-hexanediol, and butanediol.

5. The composition according to claim 1, characterized in that, The solvent is deionized water.

6. A method for preparing a slightly acidic oil-controlling and repairing composition, characterized in that, The preparation method includes the following steps: Sodium DNA, chlorogenic acid, ammonium glycyrrhizate, hydroxyethylpiperazine ethane sulfonic acid, succinic acid, glycerin, 1,2-hexanediol, carrageenan, and deionized water were mixed evenly and then sterilized to obtain the micro-acid oil-controlling and repairing composition.

7. The preparation method according to claim 6, characterized in that, The mass ratio of sodium DNA, chlorogenic acid, succinic acid, ammonium glycyrrhizate and hydroxyethylpiperazine ethanesulfonic acid is (8-11):(4-6):(8-12):(13-17):(48-52).

8. The preparation method according to claim 6, characterized in that, The total addition amount of sodium DNA, chlorogenic acid, succinic acid, ammonium glycyrrhizate and hydroxyethylpiperazine ethanesulfonic acid in the micro-acid oil-controlling and repairing composition is 5-10 wt%.

9. The use of the composition according to any one of claims 1-5 in the preparation of skin care products and hair care products with oil-controlling effects.

10. The application according to claim 9, characterized in that, The skincare products mentioned are serums, lotions, creams, or masks; The hair products mentioned are shampoos, hair styling sprays, hair styling waters, hair styling creams, or hair styling gels.

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