Anti-allergic and soothing composition and application thereof in skin care products

Abstract

The invention provides an antiallergic and soothing composition and application thereof in skin care products. The composition is prepared from the following components in parts by mass: 6 to 8 parts of shea butter, 1 to 3 parts of composite ceramide and 10 to 15 parts of patrinia villosa juss extract. The patrinia villosa juss is extracted by adopting microbial fermentation and ethyl acetate extraction technologies, active substances in the patrinia villosa juss are fully extracted, the obtained patrinia villosa juss extract, shea butter and composite ceramide have a synergistic effect, and anti-allergy and soothing effects are provided for skin.

Description

Technical Field

[0001] This invention belongs to the field of cosmetics, specifically relating to an anti-allergic and soothing composition. Background Technology

[0002] In recent years, with increasing environmental pollution, rising life pressures, and changing lifestyles, skin allergies and sensitivity have become increasingly prominent, drawing public attention. While traditional methods for treating allergies and soothing the skin have some effect, they often have side effects and limited duration of effectiveness. Therefore, developing natural, gentle, and long-lasting anti-allergy and soothing skin products has become a research hotspot in the cosmetics and pharmaceutical fields.

[0003] White patrinia (Lobularia maritima), also known as sweet patrinia, is a cruciferous herbaceous plant that has garnered widespread attention for its unique aroma and potential bioactivity. Its rich content of natural active substances, such as flavonoids, sterols, and volatile oils, possesses various bioactivities including antioxidant, anti-inflammatory, and antibacterial properties, providing a natural source for developing anti-allergy and skin-soothing products.

[0004] Patrinia scabiosifolia extract has been widely used in cosmetics and other personal care products as a skin protectant. Its main effects include soothing the skin, reducing inflammation, and relieving allergy symptoms. Studies have shown that the flavonoids in Patrinia scabiosifolia extract have significant anti-inflammatory and antioxidant effects, inhibiting the release of inflammatory factors and reducing allergic reactions. In addition, its sterols also help regulate the skin barrier function and relieve dryness and itching.

[0005] The application of Patrinia scabiosifolia extract in skincare products has the following advantages: Natural Source: The extract of Patrinia scabiosifolia is derived from natural plants, meeting consumers' demand for natural and safe skincare products.

[0006] Multiple benefits: Its rich active ingredients can simultaneously exert multiple effects such as anti-inflammatory, antioxidant, and antibacterial properties, meeting the diverse care needs of sensitive skin.

[0007] Gentle and non-irritating: Compared with traditional chemical ingredients, Patrinia scabiosifolia extract is less irritating to the skin and is suitable for sensitive skin.

[0008] Long-lasting effect: Its bioactive ingredients can continue to exert their effects, extending the product's effectiveness.

[0009] In practical applications, Patrinia scabiosifolia extract can be added to various skincare products, such as lotions, creams, and serums, to meet the needs of different consumers. However, its usage in cosmetics should comply with relevant regulations to ensure product safety and effectiveness.

[0010] In conclusion, *Patrinia scabiosifolia* extract, as a natural, mild, and highly effective active ingredient for anti-allergy and skin soothing, has broad application prospects. Further research and development can provide the public with safer, healthier, and more effective skincare options. Summary of the Invention

[0011] This invention provides an anti-allergy and soothing composition and its application in skin care products, which achieves anti-allergy and soothing effects through natural plant-extracted ingredients.

[0012] To achieve the above objectives, the present invention adopts the following technical solution: In a first aspect, the present invention provides an anti-allergic and soothing composition, said composition comprising the following parts by weight: 6-8 parts shea butter: 1-3 parts of compound ceramide: 10-15 parts of Patrinia scabiosifolia extract.

[0013] The composite ceramide comprises ceramide NG, ceramide E, and ceramide NS, wherein the mass ratio of ceramide NG, ceramide E, and ceramide NS is 1:2-4:1-3.

[0014] Preferably, the mass ratio of ceramide NG, ceramide E, and ceramide NS is 1:3:2.

[0015] Secondly, the present invention provides a method for preparing an extract of Patrinia scabiosifolia, the method comprising the following steps: S1. Take the whole plant of Patrinia scabiosifolia, remove impurities, wash, dry, and pulverize it to obtain Patrinia scabiosifolia powder; S2. Prepare a 60wt% mixture of patrinia scabiosifolia powder using deionized water. Perform ultra-high temperature instantaneous sterilization on the mixture. Inoculate the mixture with 10-12v / v% of mixed microbial inoculum for fermentation. The viable count of the mixed microbial inoculum should be 1×10⁻⁶. 8 -1×10 10 The concentration of CFU / mL was filtered after fermentation to obtain the fermentation filtrate. The initial fermentation pH was 5.7±0.2, the fermentation temperature was 29-32℃, and the fermentation time was 72-96h. The mixed strains were Rhodotorula glutinis CGMCC NO.3589 and Saccharomyces cerevisiae CGMCC NO.2.1364, with a live cell ratio of Rhodotorula glutinis to Saccharomyces cerevisiae of 1:0.5-0.9. S3. The fermentation filtrate was refluxed and extracted with water-saturated ethyl acetate. The extract was then filtered and concentrated under reduced pressure to remove the ethyl acetate, yielding the ester extract. The reflux extraction time was 2-5 hours, the extraction temperature was 60-75℃, the extraction pressure was atmospheric pressure, and the mass ratio of fermentation filtrate to water-saturated ethyl acetate was 1:20. S4. Freeze-dry the ester extract to obtain Patrinia scabiosifolia extract.

[0016] Thirdly, the present invention provides a cosmetic containing the anti-allergic and soothing composition described in the first aspect, wherein the cosmetic is a cream, lotion, serum or gel.

[0017] Preferably, the cosmetic is an anti-allergic and soothing lotion, the lotion containing the following components by weight percentage: Squalane: 1-5 wt% Isonononyl isononanoate: 1-5 wt% Cetearyl alcohol ether-21: 0.5-2 wt%; The anti-allergic and soothing composition described in the first aspect: 5-8 wt%; Glycerin: 1-2 wt% Phenoxyethanol: 0.7-1 wt%; The remainder is deionized water.

[0018] Fourthly, the present invention provides a method for preparing the anti-allergic and soothing emulsion described in the third aspect, the specific steps of which are as follows: T1. Squalane, isononyl isononanoate, and cetearyl alcohol ether-21 are mixed evenly at 60-80℃ to obtain the oil phase; T2. Heat deionized water to the same temperature to obtain an aqueous phase. Add the oil phase from T1 to the aqueous phase and homogenize for 3-5 minutes to obtain a mixed phase. T3. Once the mixed phase in T2 has cooled to 30-40°C, add the components of the anti-allergy and soothing composition, as well as glycerin and phenoxyethanol, and stir until homogeneous.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention innovatively combines shea butter, complex ceramides, and Patrinia scabiosifolia extract to develop a skincare composition with significant anti-allergic and soothing effects. In the preparation of the Patrinia scabiosifolia extract, the powder is first fermented using a mixed microbial culture (Rhododendron molle and Saccharomyces cerevisiae) to promote microbial metabolism and generate additional active substances. The active substances are then separated from the fermentation broth using ethyl acetate reflux extraction. This process not only preserves the natural anti-inflammatory and soothing active ingredients in Patrinia scabiosifolia, such as flavonoids, sterols, and esters, but also introduces additional microbial metabolites, further enhancing the efficacy of the extract. Experimental verification shows that this extract possesses excellent anti-inflammatory and soothing properties, and its synergistic effect with shea butter and complex ceramides significantly improves the overall efficacy of the composition. This innovative formulation provides a new solution for anti-allergic and soothing skin problems, while meeting consumers' demand for natural, gentle, and highly effective skincare products. Detailed Implementation

[0020] To better understand the present invention, the present invention will be further described below with reference to specific embodiments. The terminology used in the embodiments is for describing specific implementation schemes and does not constitute a limitation on the scope of protection of the present invention.

[0021] Unless otherwise specified, experimental methods in the following examples are generally performed under standard conditions or as recommended by the manufacturer. Unless otherwise stated, all measurements are by weight in percentages and parts.

[0022] Some of the raw materials and their sources are as follows: Shea butter (Butyrospermum Parkii): Purchased from Jining Tangyi Chemical Co., Ltd.

[0023] Ceramide E: Purchased from Xi'an Puris Biotechnology Co., Ltd.

[0024] Ceramide NG: Purchased from Xi'an Puris Biotechnology Co., Ltd.

[0025] Ceramide NS: Purchased from Xi'an Puris Biotechnology Co., Ltd.

[0026] Whole plant of Patrinia scabiosifolia: purchased from Bozhou Junwang Biotechnology Co., Ltd.

[0027] Red yeast accession number: CGMCC NO.3589 (CN201110114068.9).

[0028] Saccharomyces cerevisiae preservation number: CGMCC NO.2.1364 (CN202410516321.0).

[0029] The preparation of Patrinia scabiosifolia extract includes the following steps:

[0030] Example 1: S1. Take the whole plant of Patrinia scabiosifolia, remove impurities, wash, dry, and pulverize it to obtain Patrinia scabiosifolia powder; S2. Prepare a 60wt% mixture of patrinia scabiosifolia powder using deionized water. Perform ultra-high temperature instantaneous sterilization on the mixture. Inoculate the mixture with 12v / v% of mixed microbial inoculum for fermentation. The viable count of the mixed microbial inoculum is 1×10⁻⁶. 9 The concentration of CFU / mL was filtered after fermentation to obtain the fermentation filtrate. The initial fermentation pH was 5.7, the fermentation temperature was 30℃, and the fermentation time was 84h. The mixed strains were Rhodotorula glutinis CGMCC NO.3589 and Saccharomyces cerevisiae CGMCC NO.2.1364, with a live cell ratio of Rhodotorula glutinis to Saccharomyces cerevisiae of 1:0.7. S3. The fermentation filtrate was refluxed and extracted with water-saturated ethyl acetate. The extract was then filtered and concentrated under reduced pressure to remove the ethyl acetate, yielding the ester extract. The reflux extraction time was 3.5 h, the extraction temperature was 70 °C, the extraction pressure was atmospheric pressure, and the mass ratio of fermentation filtrate to water-saturated ethyl acetate was 1:20. S4. Freeze-dry the ester extract to obtain Patrinia scabiosifolia extract.

[0031] Example 2: S1. Take the whole plant of Patrinia scabiosifolia, remove impurities, wash, dry, and pulverize it to obtain Patrinia scabiosifolia powder; S2. Prepare a 60wt% mixture of patrinia scabiosifolia powder using deionized water. Perform ultra-high temperature instantaneous sterilization on the mixture. Inoculate the mixture with 10v / v% of mixed microbial inoculum for fermentation. The viable count of the mixed microbial inoculum is 1×10⁻⁶. 10 The concentration of CFU / mL was filtered after fermentation to obtain the fermentation filtrate. The initial fermentation pH was 5.9, the fermentation temperature was 29℃, and the fermentation time was 72h. The mixed strains were Rhodotorula glutinis CGMCC NO.3589 and Saccharomyces cerevisiae CGMCC NO.2.1364, with a live cell ratio of Rhodotorula glutinis to Saccharomyces cerevisiae of 1:0.9. S3. The fermentation filtrate was refluxed and extracted with water-saturated ethyl acetate. The extract was then filtered and concentrated under reduced pressure to remove the ethyl acetate, yielding the ester extract. The reflux extraction time was 2 hours, the extraction temperature was 75°C, the extraction pressure was atmospheric pressure, and the mass ratio of fermentation filtrate to water-saturated ethyl acetate was 1:20. S4. Freeze-dry the ester extract to obtain Patrinia scabiosifolia extract.

[0032] Example 3: S1. Take the whole plant of Patrinia scabiosifolia, remove impurities, wash, dry, and pulverize it to obtain Patrinia scabiosifolia powder; S2. Prepare a 60wt% mixture of patrinia scabiosifolia powder using deionized water. Perform ultra-high temperature instantaneous sterilization on the mixture. Inoculate the mixture with 14v / v% of mixed microbial inoculum for fermentation. The viable count of the mixed microbial inoculum is 1×10⁻⁶. 8 The concentration of CFU / mL was filtered after fermentation to obtain the fermentation filtrate. The initial fermentation pH was 5.5, the fermentation temperature was 32℃, and the fermentation time was 96h. The mixed strains were Rhodotorula glutinis CGMCC NO.3589 and Saccharomyces cerevisiae CGMCC NO.2.1364, with a live cell ratio of Rhodotorula glutinis to Saccharomyces cerevisiae of 1:0.5. S3. The fermentation filtrate was refluxed and extracted with water-saturated ethyl acetate. The extract was then filtered and concentrated under reduced pressure to remove the ethyl acetate, yielding the ester extract. The reflux extraction time was 5 hours, the extraction temperature was 60°C, and the extraction pressure was atmospheric pressure. The mass ratio of fermentation filtrate to water-saturated ethyl acetate was 1:20. S4. Freeze-dry the ester extract to obtain Patrinia scabiosifolia extract.

[0033] Comparative Example 1: Unlike Example 1, the mixed strain is only Rhodotorula glutinis CGMCC NO.3589, and the other steps are the same as in Example 1.

[0034] Comparative Example 2: Unlike Example 1, the mixed strain is only Saccharomyces cerevisiae CGMCC NO.2.1364, and the other steps are the same as in Example 1.

[0035] Comparative Example 3: Unlike Example 1, the ratio of viable cells of Rhodotorula glutinis CGMCC NO.3589 to Saccharomyces cerevisiae CGMCC NO.2.1364 in the mixed strain was 0.7:1, and the other steps were the same as in Example 1.

[0036] Comparative Example 4: Unlike Example 1, Red Yeast Rice CGMCC NO.2.703 was used instead of Red Yeast Rice CGMCC NO.3589, and the other steps were the same as in Example 1.

[0037] Comparative Example 5: Unlike Example 1, brewer's yeast CGMCC NO.2.1364 was replaced with brewer's yeast CGMCC NO.23637, and the other steps were the same as in Example 1.

[0038] An anti-allergic and soothing composition, the components of which and their mass ratios are as follows: Example 4: The mass ratio of shea butter, compound ceramide and white patrinia extract (Example 1) was 6:3:10.

[0039] Example 5: The mass ratio of shea butter, compound ceramide and white patrinia extract (Example 1) was 7:2:13.

[0040] Example 6: The mass ratio of shea butter, compound ceramide and white patrinia extract (Example 1) was 8:1:15.

[0041] Example 7: The mass ratio of shea butter, compound ceramide and white patrinia extract (Example 2) was 7:2:13.

[0042] Example 8: The mass ratio of shea butter, compound ceramide and white patrinia extract (Example 3) was 7:2:13.

[0043] Comparative Example 6: Unlike Example 5, it lacks Patrinia scabiosifolia extract and is missing shea butter and complex ceramides in a mass ratio of 7:2.

[0044] Comparative Example 7: Unlike Example 5, it lacks shea butter and is missing a compound ceramide and white patrinia extract (Example 1) in a mass ratio of 2:13.

[0045] Comparative Example 8: Unlike Example 5, it lacks compound ceramides and is missing shea butter and white patrinia extract (Example 1) in a mass ratio of 7:13.

[0046] Comparative Example 9: Unlike Example 5, the extract used was Patrinia scabiosifolia extract (Comparative Example 1).

[0047] Comparative Example 10: Unlike Example 5, the extract used was Patrinia scabiosifolia extract (Comparative Example 2).

[0048] Comparative Example 11: Unlike Example 5, the extract used was Patrinia scabiosifolia extract (Comparative Example 3).

[0049] Comparative Example 12: Unlike Example 5, the extract used was Patrinia scabiosifolia extract (Comparative Example 4).

[0050] Comparative Example 13: Unlike Example 5, the extract used was Patrinia scabiosifolia extract (Comparative Example 5).

[0051] A soothing and desensitizing lotion, the components of which and their mass percentages are shown in Table 1.

[0052] Table 1. Components and mass percentage of the anti-allergic and soothing lotion.

[0053] The preparation method of Example 9 includes the following steps: T1. Squalane, isononyl isononanoate, and cetearyl alcohol ether-21 are mixed evenly at 65°C to obtain the oil phase; T2. Heat deionized water to 65°C to obtain an aqueous phase. Add the oil phase from T1 to the aqueous phase and homogenize for 5 minutes to obtain a mixed phase. T3. Once the mixed phase in T2 has cooled to 35°C, add the components of the anti-allergy and soothing composition, as well as glycerin and phenoxyethanol, and stir until homogeneous.

[0054] The preparation methods for Examples 10-15 are the same as those for Example 9; The preparation method of Comparative Example 14 includes the following steps: T1. Squalane, isononyl isononanoate, and cetearyl alcohol ether-21 are mixed evenly at 65°C to obtain the oil phase; T2. Heat deionized water to 65°C to obtain an aqueous phase. Add the oil phase from T1 to the aqueous phase and homogenize for 5 minutes to obtain a mixed phase. T3. Once the mixed phase in T2 has cooled to 35°C, add glycerol and phenoxyethanol, and stir until homogeneous.

[0055] Security test Using the 2015 Cosmetic Safety Technical Specifications as a reference standard, the emulsions of Examples 9-15 and Comparative Example 14 were evaluated for cosmetic irritation. The test method was a skin patch test, and 80 people aged 16-65 years were randomly distributed to participate in the test, with 10 people in each group.

[0056] Test method: Place the test substance into a patch applicator at a dosage of 0.020-0.025 g. Cover the patch applicator containing the test substance onto the back or flexor side of the forearm of the subject with non-irritating adhesive tape. Gently press with the palm of your hand to ensure even adhesion to the skin surface. Leave on for 24 hours. Remove the patch applicator and observe the skin reaction 30 minutes after the pressure mark disappears. If the result is negative, observe again at 24 hours and 48 hours after the patch test.

[0057] Evaluation criteria: Grade 0: Negative reaction; Grade 1: Suspicious reaction, with only slight erythema; Grade 2: Weak positive reaction, erythema, infiltration, edema, and possible papules; Grade 3: Strong positive reaction, erythema, infiltration, edema, papules may be present, and the reaction may extend beyond the test area; Grade 4: Extremely positive reaction, with obvious erythema, severe infiltration, edema, confluent herpes, and reaction extending beyond the test area.

[0058] Test results: All subjects had negative skin reactions.

[0059] The above test results show that the anti-allergy and soothing lotion provided by the present invention is gentle and non-irritating to the skin and safe to use.

[0060] Performance testing I. Skin allergy test The xylene-induced mouse ear swelling model is an acute inflammation model widely used in the research of anti-inflammatory and anti-allergic products. In this model, xylene is applied to the ears of mice, triggering a local inflammatory response manifested as ear swelling. This inflammatory response involves the release of various inflammatory mediators such as histamine and kinins, leading to local vasodilation and increased capillary permeability, and further causing inflammatory cell infiltration and acute exudative inflammatory edema in the ears. In the research of anti-allergy and skin soothing, this model can be used to evaluate the anti-inflammatory and anti-sensitization effects of cosmetics.

[0061] The xylene-induced mouse ear swelling model was used to evaluate the skin anti-sensitization effects of the compositions of Examples 4-8 and Comparative Examples 6-13.

[0062] Test samples: The compositions in Examples 4-8 and Comparative Examples 6-13 were formulated into a 6 wt% mixture with deionized water.

[0063] Experimental animals: Kunming strain mice, SPF grade, from Guangzhou Ruige Biotechnology Co., Ltd., license number SCXK (Guangdong) 2023-0059, weighing 18-20 g, with 75 males and 75 females, a total of 150 mice. They were randomly divided into 15 groups, with 10 mice in each group, and the grouping was as follows: Blank group: 0.05 mL of xylene was evenly applied to the right ear of the mice. After 30 minutes, 2 mL of deionized water was applied. After 1 hour, the mice were sacrificed by cervical dislocation, and the ears were punched and weighed.

[0064] Positive group: 0.05 mL of xylene was evenly applied to the right ear of the mice. After 30 minutes, 0.1 g of Pipracil was applied to the right ear. After 1 hour, the mice were sacrificed by cervical dislocation, and the ears were punched and weighed.

[0065] Experimental group: 0.05 mL of xylene was evenly applied to the right ear of the mice. After 30 minutes, 2 mL of the test sample was applied to the right ear. After 1 hour, the mice were sacrificed by cervical dislocation, and the ears were punched and weighed.

[0066] Test method: Applying xylene to the ears of mice can cause local tissue inflammation, release histamine, and cause acute exudative edema in the ears. The swelling degree was calculated as the difference in the weights of the ear pieces of both ears, and the average swelling degree of each group was calculated. The results were expressed as mean ± standard deviation, and the results are shown in Table 2.

[0067] Table 2 Results of skin anti-sensitization test experimental group Mean swelling degree / mg Example 4 1.51±0.16* Example 5 1.42±0.26* Example 6 1.73±0.24* Example 7 1.67±0.33* Example 8 1.84±0.18* Comparative Example 6 4.82±0.17* Comparative Example 7 2.17±0.12* Comparative Example 8 2.06±0.22* Comparative Example 9 3.82±0.31* Comparative Example 10 2.44±0.15* Comparative Example 11 2.91±0.08* Comparative Example 12 2.11±0.41* Comparative Example 13 2.16±0.19* Blank group 5.44±0.07 positive group 0.07±0.03* Note: "*" indicates p < 0.05 compared with the blank group.

[0068] From the results of Examples 4-8 in Table 2, it can be seen that the effect of Example 5 is the best.

[0069] Comparing the results of Example 5 with those of Comparative Examples 6-8, it can be seen that the extract of Patrinia scabiosifolia described in this invention has a certain synergistic effect with shea butter and compound ceramide.

[0070] Comparing the results of Example 5 with those of Comparative Examples 9-11, it can be seen that fermenting Patrinia scabiosifolia with different strains of bacteria will bring different anti-inflammatory and soothing effects. When Patrinia scabiosifolia extract is prepared using strains of the types and dosage ratios specified in this invention, the anti-allergic and soothing composition has better anti-inflammatory and soothing effects.

[0071] Comparing the results of Example 5 with those of Comparative Examples 12-13, it can be seen that different strains of the same species can achieve different soothing effects. The data shows that the Rhodotorula glutinis CGMCC NO.3589 and Saccharomyces cerevisiae CGMCC NO.2.1364 provided by this invention have a better synergistic effect.

[0072] II. Soothing Efficacy Test Hyaluronidase inhibition test Experimental principle: Hyaluronidase can decompose mucopolysaccharides and non-specifically decompose and hydrolyze the 1,4-bond between β-N-acetylglucosamine and D-glucuronide in hyaluronic acid. The generated β-N-acetylglucosamine condenses with acetylacetone under alkaline conditions to generate the chromogen 2-methyl-3-diacetylpyrrole derivative. The chromogen reacts with Ehrich's reagent (Ehrich, p-dimethylaminobenzaldehyde alcohol solution) to produce color.

[0073] Preparation of various buffer solutions and reagents: ① Acetate buffer (pH=5.6): Dilute 1.155 mL of glacial acetic acid to 100 mL and mix well. Take 4.8 mL of this solution as solution A. Weigh 2.72 g of sodium acetate crystals, dissolve them in water, and bring the volume to 100 mL. Take 45.2 mL of this solution as solution B. Mix solutions A and B, and bring the volume to 100 mL with water. Accurately determine the pH and adjust it to 5.6 using either solution A or B.

[0074] ② Hyaluronidase solution (concentration of 1250U / mL): Weigh 10mg of hyaluronidase into a beaker and add 4mL of acetate buffer.

[0075] ③ Sodium hyaluronate solution (0.5 mg / mL): Weigh 5 mg of sodium hyaluronate into a beaker, add 10 mL of acetate buffer solution, store in a 10 mL centrifuge tube at 4 °C, and use an analytical balance for weighing.

[0076] ④ Ehrlich's reagent: It is prepared by dissolving 0.8 g of p-dimethylaminobenzaldehyde in a mixture of 15 mL hydrochloric acid and 15 mL anhydrous ethanol.

[0077] ⑤ Acetylacetone solution: Dissolve 3.5 mL of acetylacetone in 50 mL of 1.0 mol / L sodium carbonate solution. Prepare this solution fresh before use.

[0078] ⑥ Calcium chloride solution (0.25 mmol / L): Weigh 2.75 g of calcium chloride solid, dissolve it in water, and make up to 100 mL.

[0079] ⑦ Sodium hydroxide solution (0.4 mol / L): Weigh 1.6 g of sodium hydroxide solid, add water to dissolve and make up to 100 mL.

[0080] ⑧ Sample: Prepare a 6wt% sample solution of the compositions in Examples 4-8 and Comparative Examples 6-13 using deionized water.

[0081] The hyaluronidase inhibition assay was performed using the Elson-Morgan method, as shown in Table 3. 0.1 mL of calcium chloride solution and 0.5 mL of hyaluronidase solution were incubated at 37°C for 20 min. 0.5 mL of the sample mixture was added, and the incubation continued at 37°C for another 20 min. 0.5 mL of sodium hyaluronate solution was added, and the incubation was continued at 37°C for 30 min, followed by incubation at room temperature for 5 min. 0.1 mL of 0.4 mol / L NaOH solution and 0.5 mL of acetylacetone solution were added, and the mixture was heated in a boiling water bath for 15 min, followed immediately by cooling with ice water for 5 min. 1.0 mL of Ehrlich's reagent was added and diluted with 3.0 mL of anhydrous ethanol. The mixture was allowed to develop color for 20 min, and the absorbance was measured.

[0082] Table 3. Hyaluronidase Inhibition Test Reaction System Reagent / (mL) Calcium chloride solution Hyaluronidase solution sample Sodium hyaluronate solution Sodium hydroxide solution Sodium hydroxide solution Acetylacetone solution Ehrlich reagent Anhydrous ethanol A 0.1 0.5 # 0.5 0.1 0.5 1.0 3.0 B 0.1 # # 0.5 0.1 0.5 1.0 3.0 C 0.1 0.5 0.5 0.5 0.1 0.5 1.0 3.0 D 0.1 # 0.5 0.5 0.1 0.5 1.0 3.0 Note: # indicates the addition of 0.5 mL of acetate buffer (pH=5.6).

[0083] The formula for determining the inhibition rate of hyaluronidase by the sample is as follows: Hyaluronic acid inhibition rate (%) = [((AB) - (CD)) / (AB)] × 100% A - ABS value of the control group (using acetate buffer instead of sample solution); B - ABS value of the control blank group (using vinegar replacement buffer instead of sample solution and enzyme solution); C - ABS value of the test solution; D-Test blank solution ABS value (using acetate buffer instead of enzyme solution).

[0084] During the experiment, the wavelength of the A group samples was first scanned in the range of 450-700 nm to determine the maximum absorption wavelength. Then, using deionized water as a reference, the ABS values ​​of the remaining groups were measured at the maximum absorption wavelength. The results are shown in Table 4.

[0085] Table 4. Experimental results of hyaluronidase inhibition rate experimental group Hyaluronic acid inhibition rate (%) Example 4 79.36±1.13 Example 5 82.41±1.27 Example 6 74.28±0.93 Example 7 77.24±0.46 Example 8 70.32±0.17 Comparative Example 6 36.67±2.12 Comparative Example 7 61.53±1.62 Comparative Example 8 65.72±3.22 Comparative Example 9 49.35±1.15 Comparative Example 10 58.44±2.48 Comparative Example 11 53.67±2.01 Comparative Example 12 66.73±1.53 Comparative Example 13 68.25±0.95 Table 4 Results Analysis: Comparing the results of Examples 4-8 and Comparative Examples 6-13, it can be seen that Examples 4-8 all have significant inhibitory effects on hyaluronidase. Among them, Example 5 has the best inhibitory effect, indicating that the cosmetics prepared using the composition of the present invention have a better skin soothing effect.

[0086] Comparing the results of Example 5 with those of Comparative Examples 6-8, it can be seen that the extract of Patrinia scabiosifolia described in this invention has a certain synergistic effect with shea butter and compound ceramide.

[0087] Comparing the results of Example 5 with those of Comparative Examples 9-11, it can be seen that fermenting Patrinia scabiosifolia with different strains of bacteria will bring different soothing effects. When Patrinia scabiosifolia extract is prepared using strains of the types and dosage ratios specified in this invention, the anti-allergic and soothing composition has better soothing effects.

[0088] Comparing the results of Example 5 with those of Comparative Examples 12-13, it can be seen that different strains of the same species can achieve different soothing effects. The data shows that the Rhodotorula glutinis CGMCC NO.3589 and Saccharomyces cerevisiae CGMCC NO.2.1364 provided by this invention have a better synergistic effect.

[0089] The embodiments described above are some, but not all, of the embodiments of this application. The detailed description of the embodiments of this application is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

Claims

1. An anti-allergic and soothing composition, characterized in that, The composition comprises the following parts by weight: 6-8 parts shea butter; 1-3 parts of compound ceramide; 10-15 parts of extract of Patrinia scabiosifolia; The preparation method of the extract of Patrinia scabiosifolia includes the following steps: S1. Take the whole plant of Patrinia scabiosifolia, remove impurities, wash, dry, and pulverize it to obtain Patrinia scabiosifolia powder; S2. Prepare a 60wt% mixture of patrinia scabiosifolia powder using deionized water. Perform ultra-high temperature instantaneous sterilization on the mixture. Inoculate the mixture with 10-12v / v% of mixed microbial inoculum for fermentation. The viable count of the mixed microbial inoculum should be 1×10⁻⁶. 8 -1×10 10 The concentration of CFU / mL was filtered after fermentation to obtain the fermentation filtrate. The initial fermentation pH was 5.7±0.2, the fermentation temperature was 29-32℃, and the fermentation time was 72-96h. The mixed strains were Rhodotorula glutinis and Saccharomyces cerevisiae, with a live cell ratio of Rhodotorula glutinis to Saccharomyces cerevisiae of 1:0.5-0.

9. S3. The fermentation filtrate was refluxed and extracted with water-saturated ethyl acetate. The extract was then filtered and concentrated under reduced pressure to remove the ethyl acetate, yielding the ester extract. The reflux extraction time was 2-5 hours, the extraction temperature was 60-75℃, the extraction pressure was atmospheric pressure, and the mass ratio of fermentation filtrate to water-saturated ethyl acetate was 1:

20. S4. Freeze-dry the ester extract to obtain Patrinia scabiosifolia extract.

2. The composition according to claim 1, characterized in that, The composite ceramide comprises ceramide NG, ceramide E, and ceramide NS, wherein the mass ratio of ceramide NG, ceramide E, and ceramide NS is 1:2-4:1-3.

3. The composition according to claim 2, characterized in that, The mass ratio of ceramide NG, ceramide E, and ceramide NS is 1:3:

2.

4. A cosmetic comprising the composition according to any one of claims 1-3, characterized in that, The cosmetic product is a cream, lotion, serum, or gel.

5. The cosmetic product according to claim 4, characterized in that, The cosmetic product is a soothing and calming lotion.

6. A soothing and calming lotion, characterized in that, The emulsion contains the following components by weight percentage: Squalane: 1-5 wt% Isonononyl isononanoate: 1-5 wt% Cetearyl alcohol ether-21: 0.5-2 wt%; The anti-allergic and soothing composition according to any one of claims 1-3: 5-8 wt% Glycerin: 1-2 wt% Phenoxyethanol: 0.7-1 wt%; The remainder is deionized water.

7. The anti-allergic and soothing lotion according to claim 6, characterized in that, The method for preparing the emulsion includes the following steps: T1. Squalane, isononyl isononanoate, and cetearyl alcohol ether-21 are mixed evenly at 60-80℃ to obtain the oil phase; T2. Heat deionized water to the same temperature to obtain an aqueous phase. Add the oil phase from T1 to the aqueous phase and homogenize for 3-5 minutes to obtain a mixed phase. T3. Once the mixed phase in T2 has cooled to 30-40°C, add the components of the anti-allergy and soothing composition, as well as glycerin and phenoxyethanol, and stir until homogeneous.

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