Probiotic composition for enhancing immunity and preparation method thereof
By mixing traditional Chinese medicine with probiotics in a specific formula and preparing a probiotic composition, the shortcomings of existing probiotic compositions in enhancing immunity are solved, and a significant effect of improving immunity is achieved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- JIACHENG ZHIHE (BEIJING) TECHNOLOGY CO LTD
- Filing Date
- 2026-03-12
- Publication Date
- 2026-05-19
AI Technical Summary
Current probiotic compositions are not effective enough in enhancing immunity and are difficult to effectively improve human immunity.
A probiotic composition was prepared by mixing Chinese medicinal herbs such as Astragalus membranaceus, Paris polyphylla, Glycyrrhiza uralensis, Panax notoginseng, Citrus reticulata peel, Panax quinquefolius, Angelica sinensis, and Gardenia jasminoides with probiotics such as Bifidobacterium lactis, Bifidobacterium breve, Lactobacillus rhamnosus, and Lactobacillus acidophilus in a specific ratio. The effective components of the Chinese medicinal herbs were extracted by a combination of ethanol extraction and petroleum ether extraction with ultrasonic vibration technology, and probiotic microcapsules were prepared using calcium sulfate solution.
It significantly improved the regulatory effect of the probiotic composition on the immune system, improved the immunity of mice, and found that there is an upper limit to the amount of probiotics added; the effect no longer increases beyond the upper limit.
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Abstract
Description
Technical Field
[0001] This invention relates to the field of biopharmaceutical technology, and in particular to a probiotic composition for enhancing immunity and its preparation method. Background Technology
[0002] The gut microbiota is closely linked to the human immune system. Recent studies have found that improving the ecological balance of the gut microbiota can effectively enhance human immunity. Therefore, the development of probiotic compositions has become a research hotspot in the health food industry in recent years. Summary of the Invention
[0003] The technical problem to be solved by the present invention is to provide a probiotic composition for enhancing immunity and a method for preparing the same, which can overcome the shortcomings of the prior art and effectively improve immunity.
[0004] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is as follows.
[0005] A method for preparing a probiotic composition for enhancing immunity includes the following steps: (All numbers below are weight fractions.) Mix and pulverize 10-15 parts of Astragalus membranaceus, 2-3 parts of Paris polyphylla, 2-6 parts of Glycyrrhiza uralensis, 3-5 parts of Panax notoginseng, 1-2 parts of Citrus reticulata peel, 20-30 parts of Panax quinquefolius, 5-10 parts of Angelica sinensis, and 4-5 parts of Gardenia jasminoides, and then purify them to obtain composition I. Microcapsule powder was prepared using 0.02–0.05 parts of Bifidobacterium lactis, 0.02–0.05 parts of Bifidobacterium breve, 0.02–0.08 parts of Lactobacillus rhamnosus and 0.01–0.02 parts of Lactobacillus acidophilus as composition II; Composition I and Composition II are mixed at a mass ratio of 8:1 to 10:1 to obtain a probiotic composition for enhancing immunity.
[0006] Preferably, the preparation process of composition I is as follows: Mix and pulverize 10-15 parts of Astragalus membranaceus, 2-3 parts of Paris polyphylla, 2-6 parts of Glycyrrhiza uralensis, 3-5 parts of Panax notoginseng, 1-2 parts of Citrus reticulata peel, 20-30 parts of Panax quinquefolius, 5-10 parts of Angelica sinensis, and 4-5 parts of Gardenia jasminoides. The particle size of the pulverized mixture is 400-500 mesh. The pulverized mixture was added to 300 parts of 95wt% ethanol solution for extraction. After ultrasonic vibration for 3 hours, the mixture was sealed and allowed to stand for 24 hours. Impurities were filtered out, and the filtrate was distilled under reduced pressure to obtain a concentrated solution. The concentrated solution was then subjected to a second extraction using petroleum ether at a volume ratio of 10:1. Ultrasonic vibration was used for 1 hour during the extraction, followed by standing for 1 hour. After filtering out impurities, the mixture was distilled under reduced pressure to obtain a traditional Chinese medicine extract. The extract was dried and pulverized to obtain composition I.
[0007] Preferably, the ultrasonic vibration frequency is 25 kHz and the power density is 2.5 W / cm². 3 .
[0008] Preferably, when using ethanol for extraction, the temperature is controlled at 80–85°C, and when using petroleum ether for extraction, the temperature is controlled at 35–40°C.
[0009] Preferably, the preparation process of composition II is as follows: Add 0.02–0.05 parts of Bifidobacterium lactis, 0.02–0.05 parts of Bifidobacterium breve, 0.02–0.08 parts of Lactobacillus rhamnosus and 0.01–0.02 parts of Lactobacillus acidophilus to 100 parts of pure water and stir to mix. Then add 1.5–3 parts of carrageenan and 2–5 parts of maltodextrin to the mixture to prepare an intermediate solution. The intermediate solution was added dropwise to an 8wt% to 10wt% calcium sulfate solution with a mass ratio of 1:5. Stirring was maintained during the dropwise addition process, and stirring was continued for 30 minutes after the dropwise addition was completed. The mixture of intermediate solution and calcium sulfate solution was filtered with filter paper to obtain probiotic microcapsules. The probiotic microcapsules were rinsed with physiological saline and then freeze-dried to obtain composition II.
[0010] Preferably, the viable counts of Bifidobacterium lactis, Bifidobacterium breve, Lactobacillus rhamnosus, and Lactobacillus acidophilus are all 3 × 10⁻⁶. 9 CFU / g ~ 9×10 9 CFU / g.
[0011] A probiotic composition for enhancing immunity, prepared using the above-described preparation method.
[0012] The beneficial effects of adopting the above technical solution are as follows: by improving the component formulation of the probiotic composition, the present invention effectively improves the regulatory effect of the probiotic composition on the immune system. Detailed Implementation Example 1
[0013] Mix and pulverize 14 parts of Astragalus membranaceus, 2 parts of Paris polyphylla, 5 parts of Glycyrrhiza uralensis, 4 parts of Panax notoginseng, 2 parts of Citrus reticulata peel, 26 parts of Panax quinquefolius, 8 parts of Angelica sinensis, and 4 parts of Gardenia jasminoides. The particle size of the pulverized mixture is 400-500 mesh.
[0014] The pulverized mixture was extracted with 300 parts of 95wt% ethanol solution at 80℃, followed by ultrasonic vibration for 3 hours, then sealed and allowed to stand for 24 hours. Impurities were filtered out, and the filtrate was distilled under reduced pressure to obtain a concentrated solution. The concentrated solution was then subjected to a second extraction with petroleum ether at 40℃, with a volume ratio of petroleum ether to concentrated solution of 10:1. Ultrasonic vibration was used for 1 hour during extraction, followed by standing for 1 hour, then impurities were filtered out and the mixture was distilled under reduced pressure to obtain a herbal extract. The herbal extract was dried and pulverized to obtain composition I. The ultrasonic vibration frequency was 25kHz, and the power density was 2.5W / cm³. 3 .
[0015] 0.03 parts of *Bifidobacterium lactis*, 0.03 parts of *Bifidobacterium breve*, 0.05 parts of *Lactobacillus rhamnosus*, and 0.01 parts of *Lactobacillus acidophilus* were added to 100 parts of purified water and mixed. Then, 2 parts of carrageenan and 4 parts of maltodextrin were added to the mixture to prepare an intermediate solution. The viable count of each bacterial strain was 5 × 10⁻⁶. 9 CFU / g.
[0016] The intermediate solution was added dropwise to a 9 wt% calcium sulfate solution at a mass ratio of 1:5. Stirring was maintained throughout the addition process, and continued for 30 minutes after the addition was complete. The stirring speed was 350 rad / min during the addition and 800 rad / min afterward.
[0017] The mixture of intermediate solution and calcium sulfate solution was filtered with filter paper to obtain probiotic microcapsules. The probiotic microcapsules were rinsed with physiological saline and then freeze-dried to obtain composition II.
[0018] Composition I and Composition II were mixed at a mass ratio of 8:1 to obtain a probiotic composition for enhancing immunity. Example 2
[0019] Mix and pulverize 12 parts of Astragalus membranaceus, 3 parts of Paris polyphylla, 4 parts of Glycyrrhiza uralensis, 5 parts of Panax notoginseng, 1 part of Citrus reticulata peel, 21 parts of Panax quinquefolius, 7 parts of Angelica sinensis, and 4 parts of Gardenia jasminoides. The particle size of the pulverized mixture is 400-500 mesh.
[0020] The pulverized mixture was extracted with 300 parts of 95wt% ethanol solution at 85℃. After ultrasonic vibration for 3 hours, the mixture was sealed and allowed to stand for 24 hours. Impurities were filtered out, and the filtrate was distilled under reduced pressure to obtain a concentrated solution. The concentrated solution was then subjected to a second extraction with petroleum ether at 35℃, with a volume ratio of petroleum ether to concentrated solution of 10:1. Ultrasonic vibration was used for 1 hour during extraction, followed by standing for 1 hour. Impurities were then filtered out, and the mixture was distilled under reduced pressure to obtain a herbal extract. The herbal extract was dried and pulverized to obtain composition I. The ultrasonic vibration frequency was 25kHz, and the power density was 2.5W / cm³. 3 .
[0021] 0.02 parts of *Bifidobacterium lactis*, 0.05 parts of *Bifidobacterium breve*, 0.08 parts of *Lactobacillus rhamnosus*, and 0.02 parts of *Lactobacillus acidophilus* were added to 100 parts of purified water and mixed. Then, 3 parts of carrageenan and 3 parts of maltodextrin were added to the mixture to prepare an intermediate solution. The viable count of each bacterial strain was 4 × 10⁻⁶. 9 CFU / g.
[0022] The intermediate solution was added dropwise to an 8 wt% calcium sulfate solution at a mass ratio of 1:5. Stirring was maintained throughout the addition process, and continued for 30 minutes after the addition was complete. The stirring speed was 350 rad / min during the addition process and 800 rad / min after the addition was complete.
[0023] The mixture of intermediate solution and calcium sulfate solution was filtered with filter paper to obtain probiotic microcapsules. The probiotic microcapsules were rinsed with physiological saline and then freeze-dried to obtain composition II.
[0024] Composition I and Composition II were mixed at a mass ratio of 10:1 to obtain a probiotic composition for enhancing immunity.
[0025] Comparative Example 1 Mix and pulverize 5 parts licorice, 4 parts Panax notoginseng, 2 parts dried tangerine peel, 26 parts American ginseng, 8 parts Angelica sinensis, and 4 parts Gardenia jasminoides. The particle size of the pulverized mixture is 400-500 mesh.
[0026] The pulverized mixture was extracted with 300 parts of 95wt% ethanol solution at 80℃, followed by ultrasonic vibration for 3 hours, then sealed and allowed to stand for 24 hours. Impurities were filtered out, and the filtrate was distilled under reduced pressure to obtain a concentrated solution. The concentrated solution was then subjected to a second extraction with petroleum ether at 40℃, with a volume ratio of petroleum ether to concentrated solution of 10:1. Ultrasonic vibration was used for 1 hour during extraction, followed by standing for 1 hour, then impurities were filtered out and the mixture was distilled under reduced pressure to obtain a herbal extract. The herbal extract was dried and pulverized to obtain composition I. The ultrasonic vibration frequency was 25kHz, and the power density was 2.5W / cm³.3 .
[0027] 0.03 parts of *Bifidobacterium lactis*, 0.03 parts of *Bifidobacterium breve*, 0.05 parts of *Lactobacillus rhamnosus*, and 0.01 parts of *Lactobacillus acidophilus* were added to 100 parts of purified water and mixed. Then, 2 parts of carrageenan and 4 parts of maltodextrin were added to the mixture to prepare an intermediate solution. The viable count of each bacterial strain was 5 × 10⁻⁶. 9 CFU / g.
[0028] The intermediate solution was added dropwise to a 9 wt% calcium sulfate solution at a mass ratio of 1:5. Stirring was maintained throughout the addition process, and continued for 30 minutes after the addition was complete. The stirring speed was 350 rad / min during the addition and 800 rad / min afterward.
[0029] The mixture of intermediate solution and calcium sulfate solution was filtered with filter paper to obtain probiotic microcapsules. The probiotic microcapsules were rinsed with physiological saline and then freeze-dried to obtain composition II.
[0030] Composition I and Composition II were mixed at a mass ratio of 8:1 to obtain a probiotic composition for enhancing immunity.
[0031] Comparative Example 2 Mix and pulverize 14 parts of Astragalus membranaceus, 5 parts of Glycyrrhiza uralensis, 4 parts of Panax notoginseng, 2 parts of Citrus reticulata peel, 26 parts of Panax quinquefolius, 8 parts of Angelica sinensis, and 4 parts of Gardenia jasminoides. The particle size of the pulverized mixture is 400-500 mesh.
[0032] The pulverized mixture was extracted with 300 parts of 95wt% ethanol solution at 80℃, followed by ultrasonic vibration for 3 hours, then sealed and allowed to stand for 24 hours. Impurities were filtered out, and the filtrate was distilled under reduced pressure to obtain a concentrated solution. The concentrated solution was then subjected to a second extraction with petroleum ether at 40℃, with a volume ratio of petroleum ether to concentrated solution of 10:1. Ultrasonic vibration was used for 1 hour during extraction, followed by standing for 1 hour, then impurities were filtered out and the mixture was distilled under reduced pressure to obtain a herbal extract. The herbal extract was dried and pulverized to obtain composition I. The ultrasonic vibration frequency was 25kHz, and the power density was 2.5W / cm³. 3 .
[0033] 0.03 parts of *Bifidobacterium lactis*, 0.03 parts of *Bifidobacterium breve*, 0.05 parts of *Lactobacillus rhamnosus*, and 0.01 parts of *Lactobacillus acidophilus* were added to 100 parts of purified water and mixed. Then, 2 parts of carrageenan and 4 parts of maltodextrin were added to the mixture to prepare an intermediate solution. The viable count of each bacterial strain was 5 × 10⁻⁶. 9 CFU / g.
[0034] The intermediate solution was added dropwise to a 9 wt% calcium sulfate solution at a mass ratio of 1:5. Stirring was maintained throughout the addition process, and continued for 30 minutes after the addition was complete. The stirring speed was 350 rad / min during the addition and 800 rad / min afterward.
[0035] The mixture of intermediate solution and calcium sulfate solution was filtered with filter paper to obtain probiotic microcapsules. The probiotic microcapsules were rinsed with physiological saline and then freeze-dried to obtain composition II.
[0036] Composition I and Composition II were mixed at a mass ratio of 8:1 to obtain a probiotic composition for enhancing immunity.
[0037] Comparative Example 3 Mix and pulverize 2 parts of Paris polyphylla, 5 parts of licorice, 4 parts of Panax notoginseng, 2 parts of dried tangerine peel, 26 parts of American ginseng, 8 parts of Angelica sinensis, and 4 parts of Gardenia jasminoides. The particle size of the pulverized mixture is 400-500 mesh.
[0038] The pulverized mixture was extracted with 300 parts of 95wt% ethanol solution at 80℃, followed by ultrasonic vibration for 3 hours, then sealed and allowed to stand for 24 hours. Impurities were filtered out, and the filtrate was distilled under reduced pressure to obtain a concentrated solution. The concentrated solution was then subjected to a second extraction with petroleum ether at 40℃, with a volume ratio of petroleum ether to concentrated solution of 10:1. Ultrasonic vibration was used for 1 hour during extraction, followed by standing for 1 hour, then impurities were filtered out and the mixture was distilled under reduced pressure to obtain a herbal extract. The herbal extract was dried and pulverized to obtain composition I. The ultrasonic vibration frequency was 25kHz, and the power density was 2.5W / cm³. 3 .
[0039] 0.03 parts of *Bifidobacterium lactis*, 0.03 parts of *Bifidobacterium breve*, 0.05 parts of *Lactobacillus rhamnosus*, and 0.01 parts of *Lactobacillus acidophilus* were added to 100 parts of purified water and mixed. Then, 2 parts of carrageenan and 4 parts of maltodextrin were added to the mixture to prepare an intermediate solution. The viable count of each bacterial strain was 5 × 10⁻⁶. 9 CFU / g.
[0040] The intermediate solution was added dropwise to a 9 wt% calcium sulfate solution at a mass ratio of 1:5. Stirring was maintained throughout the addition process, and continued for 30 minutes after the addition was complete. The stirring speed was 350 rad / min during the addition and 800 rad / min afterward.
[0041] The mixture of intermediate solution and calcium sulfate solution was filtered with filter paper to obtain probiotic microcapsules. The probiotic microcapsules were rinsed with physiological saline and then freeze-dried to obtain composition II.
[0042] Composition I and Composition II were mixed at a mass ratio of 8:1 to obtain a probiotic composition for enhancing immunity.
[0043] Comparative Example 4 Mix and pulverize 14 parts of Astragalus membranaceus, 2 parts of Paris polyphylla, 5 parts of Glycyrrhiza uralensis, 4 parts of Panax notoginseng, 2 parts of Citrus reticulata peel, 26 parts of Panax quinquefolius, 8 parts of Angelica sinensis, and 4 parts of Gardenia jasminoides. The particle size of the pulverized mixture is 400-500 mesh.
[0044] The pulverized mixture was extracted with 300 parts of 95wt% ethanol solution at 80℃, followed by ultrasonic vibration for 3 hours, then sealed and allowed to stand for 24 hours. Impurities were filtered out, and the filtrate was distilled under reduced pressure to obtain a concentrated solution. The concentrated solution was then subjected to a second extraction with petroleum ether at 40℃, with a volume ratio of petroleum ether to concentrated solution of 10:1. Ultrasonic vibration was used for 1 hour during extraction, followed by standing for 1 hour, then impurities were filtered out and the mixture was distilled under reduced pressure to obtain a herbal extract. The herbal extract was dried and pulverized to obtain composition I. The ultrasonic vibration frequency was 25kHz, and the power density was 2.5W / cm³. 3 .
[0045] 0.1 parts of *Bifidobacterium lactis*, 0.1 parts of *Bifidobacterium breve*, 0.2 parts of *Lactobacillus rhamnosus*, and 0.05 parts of *Lactobacillus acidophilus* were added to 100 parts of purified water and mixed. Then, 2 parts of carrageenan and 4 parts of maltodextrin were added to the mixture to prepare an intermediate solution. The viable count of each bacterial strain was 5 × 10⁻⁶. 9 CFU / g.
[0046] The intermediate solution was added dropwise to a 9 wt% calcium sulfate solution at a mass ratio of 1:5. Stirring was maintained throughout the addition process, and continued for 30 minutes after the addition was complete. The stirring speed was 350 rad / min during the addition and 800 rad / min afterward.
[0047] The mixture of intermediate solution and calcium sulfate solution was filtered with filter paper to obtain probiotic microcapsules. The probiotic microcapsules were rinsed with physiological saline and then freeze-dried to obtain composition II.
[0048] Composition I and Composition II were mixed at a mass ratio of 8:1 to obtain a probiotic composition for enhancing immunity.
[0049] A comparative experiment was conducted using the probiotic compositions prepared in the above examples and comparative examples. Sixty mice were selected and divided into six groups. First, the average IgG level in the blood of each group of mice was measured. Then, the mice were fed the corresponding probiotic compositions prepared in the examples or comparative examples twice daily, 0.5g each time. After three months of continuous feeding, the average IgG level in the blood of each group of mice was measured again. The results are as follows:
[0050] As shown in the table above, the innovative addition of Astragalus membranaceus and Paris polyphylla to the probiotic composition in this invention can significantly improve the absorption of probiotics in mice and enhance their immunity. The experiment also revealed an upper limit to the effectiveness of the added probiotic amount; exceeding this limit and further increasing the amount of probiotics did not significantly improve the immune-enhancing effect.
[0051] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of this invention is defined by the appended claims and their equivalents.
Claims
1. A method for preparing a probiotic composition for enhancing immunity, characterized in that... Includes the following steps: All the following portions are by weight. Mix and pulverize 10-15 parts of Astragalus membranaceus, 2-3 parts of Paris polyphylla, 2-6 parts of Glycyrrhiza uralensis, 3-5 parts of Panax notoginseng, 1-2 parts of Citrus reticulata peel, 20-30 parts of Panax quinquefolius, 5-10 parts of Angelica sinensis, and 4-5 parts of Gardenia jasminoides, and then purify them to obtain composition I. Microcapsule powder was prepared using 0.02–0.05 parts of Bifidobacterium lactis, 0.02–0.05 parts of Bifidobacterium breve, 0.02–0.08 parts of Lactobacillus rhamnosus and 0.01–0.02 parts of Lactobacillus acidophilus as composition II; Composition I and Composition II are mixed at a mass ratio of 8:1 to 10:1 to obtain a probiotic composition for enhancing immunity.
2. The method for preparing the probiotic composition for enhancing immunity according to claim 1, characterized in that: The preparation process of composition I is as follows: Mix and pulverize 10-15 parts of Astragalus membranaceus, 2-3 parts of Paris polyphylla, 2-6 parts of Glycyrrhiza uralensis, 3-5 parts of Panax notoginseng, 1-2 parts of Citrus reticulata peel, 20-30 parts of Panax quinquefolius, 5-10 parts of Angelica sinensis, and 4-5 parts of Gardenia jasminoides. The particle size of the pulverized mixture is 400-500 mesh. The pulverized mixture was added to 300 parts of 95wt% ethanol solution for extraction. After ultrasonic vibration for 3 hours, the mixture was sealed and allowed to stand for 24 hours. Impurities were filtered out, and the filtrate was distilled under reduced pressure to obtain a concentrated solution. The concentrated solution was then subjected to a second extraction using petroleum ether at a volume ratio of 10:
1. Ultrasonic vibration was used for 1 hour during the extraction, followed by standing for 1 hour. After filtering out impurities, the mixture was distilled under reduced pressure to obtain a traditional Chinese medicine extract. The extract was dried and pulverized to obtain composition I.
3. The method for preparing the probiotic composition for enhancing immunity according to claim 2, characterized in that: The ultrasonic vibration frequency is 25 kHz, and the power density is 2.5 W / cm². 3 .
4. The method for preparing the probiotic composition for enhancing immunity according to claim 3, characterized in that: When using ethanol for extraction, the temperature should be controlled at 80–85°C; when using petroleum ether for extraction, the temperature should be controlled at 35–40°C.
5. The method for preparing the probiotic composition for enhancing immunity according to claim 1, characterized in that: The preparation process of composition II is as follows: Add 0.02–0.05 parts of Bifidobacterium lactis, 0.02–0.05 parts of Bifidobacterium breve, 0.02–0.08 parts of Lactobacillus rhamnosus and 0.01–0.02 parts of Lactobacillus acidophilus to 100 parts of pure water and stir to mix. Then add 1.5–3 parts of carrageenan and 2–5 parts of maltodextrin to the mixture to prepare an intermediate solution. The intermediate solution was added dropwise to an 8wt% to 10wt% calcium sulfate solution with a mass ratio of 1:
5. Stirring was maintained during the dropwise addition process, and stirring was continued for 30 minutes after the dropwise addition was completed. The mixture of intermediate solution and calcium sulfate solution was filtered with filter paper to obtain probiotic microcapsules. The probiotic microcapsules were rinsed with physiological saline and then freeze-dried to obtain composition II.
6. The method for preparing the probiotic composition for enhancing immunity according to claim 5, characterized in that: The viable counts of Bifidobacterium lactis, Bifidobacterium breve, Lactobacillus rhamnosus, and Lactobacillus acidophilus were all 3 × 10⁻⁶. 9 CFU / g ~ 9×10 9 CFU / g.
7. The method for preparing the probiotic composition for enhancing immunity according to claim 5, characterized in that: The stirring speed was 350 rad / min during the dripping process and 800 rad / min after the dripping was completed.
8. A probiotic composition for enhancing immunity, characterized in that: Prepared using the preparation method described in any one of claims 1-7.