Maca, ginseng and cistanche composition, oral preparation and application thereof

Abstract

The invention provides a maca, ginseng and cistanche deserticola composition. The maca, ginseng and cistanche deserticola composition is prepared from maca powder, a ginseng extract, a fructus lycii extract, a cistanche deserticola extract, ground cinnamon, a folium cortex eucommiae extract and edible salt. The invention further provides an oral formulation and a food product comprising the composition. The invention also provides application of the composition or the preparation in preparation of health-care foods or medicines for relieving physical fatigue. The composition provided by the invention can comprehensively play a role in relieving fatigue by increasing the content of liver glycogen in liver tissue and reducing lactic acid accumulation after exercise, further can improve the content of testosterone in serum, has the potential of improving reproduction, and has important market value and development prospect.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine and food technology, specifically relating to a maca-ginseng-cistanche composition and its oral preparation, as well as the application of the composition and preparation in the preparation of food, health food and medicine. Technical Background With the fast pace of modern life and increased work intensity, physical fatigue has become one of the main sub-health conditions affecting public health. Currently, the mainstream anti-fatigue foods on the market are energy drinks, which mainly rely on caffeine as their core active ingredient. They temporarily increase alertness by stimulating the central nervous system, thus "masking drowsiness" rather than "eliminating fatigue." These products fail to systematically address the root mechanisms of physical fatigue, and long-term reliance on caffeine may lead to cumulative fatigue, creating a vicious cycle of "stimulation-exhaustion." Furthermore, as a Class III psychotropic drug, excessive caffeine intake may cause adverse reactions such as palpitations, high blood pressure, anxiety, insomnia, and gastrointestinal discomfort.

[0002] Furthermore, closely related to physical fatigue, the prevalence of infertility is showing a rising trend globally. Worldwide, approximately 7% of couples suffer from infertility, and declining fertility among men of reproductive age has become a pressing societal problem. Numerous studies have shown that after age 30, men's bioavailable testosterone levels decline linearly, and effective and safe interventions are lacking. Currently, researchers and industries both domestically and internationally are actively seeking natural ingredients derived from food and medicinal resources to address physical fatigue and further male reproductive issues. Summary of the Invention

[0003] In order to overcome the shortcomings of the prior art, the present invention provides a maca and ginseng composition to relieve physical fatigue and further improve reproductive capacity.

[0004] In a first aspect, the present invention provides a maca-ginseng-cistanche composition, comprising the following components in parts by weight: 40-60 parts maca powder, 20-30 parts component A, 20-30 parts component B, 20-30 parts component C, 10-20 parts cinnamon powder, 5-8 parts Eucommia ulmoides leaf extract, and 3-6 parts edible salt; wherein, component A comprises ginseng extract; component B comprises wolfberry extract; and component C comprises cistanche extract. The composition is formed by directly mixing the above-mentioned raw materials.

[0005] The extracts used in this invention are obtained by mixing Chinese herbal raw materials with water at a mass ratio of 1:10~20, preferably 1:20, followed by hot extraction, filtration of insoluble matter, collection of filtrate, and drying.

[0006] The composition provided by this invention is formulated with reference to the traditional Chinese medicine theory of properties and meridian tropism, combined with modern pharmacological experiments, and is rationally combined. Specifically: in the composition, maca, which nourishes the kidneys and replenishes essence, is the principal ingredient; the assistant ingredients consist of component A, which tonifies kidney qi; component B, which tonifies kidney yin; and component C, which tonifies kidney yang; cinnamon, which guides fire back to its source; and eucommia bark and salt, which enter the kidneys and strengthen the waist and knees, are the guiding ingredients. Based on this, the dosage of each component is further optimized to ensure that the components work together to achieve synergistic effects and a balanced regulatory effect.

[0007] The raw material used in this invention, maca powder, is a new food ingredient (new resource food). Specifically, it refers to powder made from the rhizome of artificially cultivated maca (Lepidium meyenii Walp), a plant of the Brassicaceae family, through slicing, drying, and pulverizing processes.

[0008] The ginseng (artificially cultivated) used in this invention is a new food ingredient (new resource food), specifically referring to ginseng, a plant of the Araliaceae family. Panax ginseng The dried roots and rhizomes of CA Mey. According to the Chinese Pharmacopoeia (2025), ginseng is sweet, slightly bitter, and slightly warm in nature; it enters the spleen, lung, heart, and kidney meridians; it has the effects of greatly replenishing vital energy, restoring pulse and consolidating the body, tonifying the spleen and benefiting the lungs, generating fluids and nourishing blood, calming the mind and improving intelligence; it is mainly used for physical weakness and impending collapse, cold limbs and weak pulse, spleen deficiency and poor appetite, lung deficiency and cough, thirst due to fluid depletion, internal heat and thirst, deficiency of qi and blood, chronic illness and emaciation, palpitations and insomnia, impotence and cold uterus.

[0009] The raw material used in this invention is wolfberry, a substance that is traditionally both a food and a Chinese medicinal herb, specifically referring to *Lycium barbarum*, a plant belonging to the Solanaceae family. Lyciumm barbarum The dried, ripe fruit of *Lycium barbarum* (L.) is a substance that is traditionally used as both food and a Chinese medicinal herb. According to the Chinese Pharmacopoeia (2025), wolfberry is sweet and neutral in nature, and enters the liver and kidney meridians; it has the effects of nourishing the liver and kidneys, benefiting essence and improving eyesight; it is mainly used for deficiency of essence and blood, soreness of the waist and knees, dizziness and tinnitus, impotence and seminal emission, internal heat and thirst, blood deficiency and chlorosis, and blurred vision.

[0010] The raw material used in this invention, *Cistanche deserticola*, is a substance that is traditionally both a food and a Chinese medicinal herb; specifically, it refers to *Cistanche deserticola*, a plant belonging to the Orobanchaceae family. Cistanche deserticola The dried, fleshy stem with scales of YC Ma. According to the Chinese Pharmacopoeia (2025), Cistanche deserticola is sweet, salty, and warm in nature; it enters the kidney and large intestine meridians; it has the functions of tonifying kidney yang, nourishing essence and blood, and moistening the intestines to relieve constipation; it is mainly used for kidney yang deficiency, deficiency of essence and blood, impotence and infertility, soreness and weakness of the waist and knees, weakness of muscles and bones, and constipation due to intestinal dryness.

[0011] The cinnamon used in this invention is a substance that is traditionally both a food and a Chinese medicinal herb, specifically referring to the cinnamon plant (Cinnamomum cassia). Cinnamomum cassiaThe dried bark of Presl. According to the Chinese Pharmacopoeia (2025), cinnamon is pungent, sweet, and very hot in nature; it enters the kidney, spleen, heart, and liver meridians; it has the effects of tonifying fire and assisting yang, guiding fire back to its source, dispelling cold and relieving pain, and warming and unblocking the meridians; it is mainly used for impotence and cold uterus, cold pain in the waist and knees, kidney deficiency causing asthma, floating of deficient yang, dizziness and red eyes, cold pain in the heart and abdomen, vomiting and diarrhea due to deficiency and cold, abdominal pain due to cold hernia, dysmenorrhea and amenorrhea. The cinnamon powder mentioned above is a powder made from cinnamon through the processes of peeling, slicing, drying, and pulverizing.

[0012] The raw material used in this invention, Eucommia ulmoides leaves, is a substance that is traditionally both a food and a Chinese medicinal material; specifically, it refers to Eucommia ulmoides, a plant of the Eucommiaceae family. Eucommia ulmoides The dried leaves of Oliv. According to the Chinese Pharmacopoeia (2025), Eucommia ulmoides leaves are slightly pungent and warm in nature; they enter the liver and kidney meridians; they have the effects of tonifying the liver and kidneys and strengthening tendons and bones; they are mostly used for liver and kidney deficiency, dizziness, soreness of the waist and knees, and weakness of tendons and bones.

[0013] The raw material used in this invention is edible salt, a common condiment, in which the sodium chloride content is not less than 95%.

[0014] In a preferred embodiment of the present invention, component A is composed of ginseng extract and cordyceps militaris powder mixed in a mass ratio of (7~10):(3~6). In the preferred embodiment provided by the present invention, component A, as a kidney-tonifying agent, is composed of ginseng extract and cordyceps militaris powder mixed in a specific ratio, which can synergistically achieve the function of tonifying kidney qi, thereby improving the overall efficacy of the composition.

[0015] The raw material used in this invention is Cordyceps militaris ( Cordyceps militaris (L.ex Fr.) Link.) is a new food ingredient (new resource food), a fungus belonging to the Clavicipitaceae family and the Cordyceps genus, also known as North Cordyceps sinensis. The Cordyceps militaris powder is a powdered product made from Cordyceps militaris fruiting bodies through drying, pulverizing, and sieving processes. Its preparation can refer to the industry standard GH / T1443-2023.

[0016] In a preferred embodiment of the present invention, component B is composed of Lycium barbarum extract, mulberry extract, Polygonatum sibiricum extract, and Dioscorea opposita extract in a mass ratio of (6-8):(5-7):(6-8):(4-6). In this preferred embodiment, component B, serving as the kidney-tonifying yin-nourishing agent, is composed of Lycium barbarum extract, mulberry extract, Polygonatum sibiricum extract, and Dioscorea opposita extract in a specific ratio, which can synergistically achieve the kidney-tonifying yin function, thereby improving the overall efficacy of the composition.

[0017] The mulberry used in this invention is the fruit of a plant in the genus *Morus* of the family Moraceae. It is a health food native to my country with a cultivation history of over a thousand years. According to the *Compendium of Materia Medica*, "Mulberry juice can relieve alcohol poisoning, promote urination, reduce swelling, nourish yin and replenish blood, and is used for liver and kidney deficiency, deficiency of essence and blood, dizziness, blurred vision, tinnitus, insomnia, premature graying of hair, etc." The raw material used in this invention, Polygonatum sibiricum, is a substance that is traditionally both a food and a Chinese medicinal herb; specifically, it refers to Polygonatum sibiricum, a plant belonging to the Liliaceae family. Polygonatum sibiricum The dried rhizome of Red. According to the Chinese Pharmacopoeia (2025), Polygonatum is sweet and neutral in nature; it enters the spleen, lung and kidney meridians; it has the functions of replenishing qi and nourishing yin, strengthening the spleen, moistening the lungs and benefiting the kidneys; it is mostly used for spleen and stomach qi deficiency, fatigue, stomach yin deficiency, dry mouth and poor appetite, lung deficiency and dry cough, consumptive cough with hemoptysis, deficiency of essence and blood, soreness and weakness of waist and knees, premature graying of hair, internal heat and thirst.

[0018] The raw material used in this invention, yam, is a substance that is traditionally both a food and a Chinese medicinal material, specifically referring to the plant *Dioscorea opposita* (Dioscoreaceae family). Dioscorea opposita The dried rhizome of Thunb. Yam is sweet and neutral in nature; it enters the spleen, lung, and kidney meridians; it has the effects of tonifying the spleen and stomach, promoting body fluid production and benefiting the lungs, and tonifying the kidneys and astringing essence; it is often used for spleen deficiency with poor appetite, chronic diarrhea, lung deficiency with cough and asthma, kidney deficiency with seminal emission, leukorrhea, frequent urination, and thirst due to deficiency heat.

[0019] In a preferred embodiment of the present invention, component C is composed of Cistanche deserticola extract and Rubus idaeus extract mixed in a mass ratio of (5-8):(3-6). In the preferred embodiment provided by the present invention, component C, as an adjuvant for tonifying kidney yang, is composed of Cistanche deserticola extract and Rubus idaeus extract mixed in a specific ratio, which can synergistically achieve the function of tonifying kidney yang, thereby improving the overall efficacy of the composition.

[0020] The raw material used in this invention, raspberry, is a substance that is traditionally both a food and a Chinese medicinal herb; specifically, it refers to the Chinese raspberry (Rubus idaeus), a plant belonging to the Rosaceae family. Rubus chingii The dried fruit of Hu. According to the Chinese Pharmacopoeia (2025), raspberry is sweet, sour and warm in nature; it enters the liver, kidney and bladder meridians; it has the effects of tonifying the kidney and consolidating essence and reducing urination, nourishing the liver and improving eyesight; it is often used for seminal emission, frequent urination, impotence and premature ejaculation, and blurred vision.

[0021] In one specific embodiment of the present invention, the composition comprises the following components in parts by weight: 50-55 parts maca powder, 18-20 parts ginseng extract, 8-10 parts cordyceps powder, 6-8 parts wolfberry extract, 5-7 parts mulberry extract, 6-8 parts polygonatum extract, 4-6 parts yam extract, 12-14 parts cistanche extract, 8-10 parts raspberry extract, 12-15 parts cinnamon powder, 6-7 parts eucommia leaf extract, and 4-5 parts edible salt.

[0022] In a second aspect, the present invention provides an oral preparation of maca and ginseng, wherein the maca and ginseng composition described in the first aspect, and an appropriate amount of excipients acceptable in the fields of food, health food or pharmaceuticals.

[0023] In one specific embodiment of the present invention, the oral preparation is a tablet, preferably a regular tablet, lozenge, chewable tablet, dispersible tablet or effervescent tablet.

[0024] In one specific embodiment of the present invention, the oral preparation is a capsule, preferably a hard capsule or a soft capsule.

[0025] In one specific embodiment of the present invention, the oral preparation is a granule, preferably a soluble granule, a suspension granule, or an effervescent granule.

[0026] In one specific embodiment of the present invention, the oral preparation is a powder.

[0027] In one specific embodiment of the present invention, the oral preparation is a paste.

[0028] In one specific embodiment of the present invention, the oral preparation is a candy, preferably selected from hard candies, hard-filled candies, cream candies, gel candies, gum-based candies, aerated candies, or compressed candies. When the oral preparation is a compressed candy, xylitol is preferably used as the sweetener.

[0029] In one specific embodiment of the present invention, the oral preparation is a liquid preparation, preferably a syrup, oral liquid or beverage.

[0030] Thirdly, the present invention provides a food product comprising the maca and ginseng composition described in the first aspect or the oral preparation of maca and ginseng described in the second aspect.

[0031] The composition provided by this invention uses raw materials from substances that are both food and traditional Chinese medicine as published by health administration departments, new food raw materials (new resource foods), and foods or condiments consumed in daily life. It can be used in food, has high safety, and low toxicity and side effects.

[0032] Fourthly, the present invention provides the application of the maca and ginseng composition described in the first aspect or the oral preparation of maca and ginseng described in the second aspect in the preparation of health foods that relieve physical fatigue.

[0033] As a preferred embodiment of the present invention, the composition achieves the effect of relieving physical fatigue by increasing the content of liver glycogen in liver tissue.

[0034] As a preferred embodiment of the present invention, the composition relieves physical fatigue by reducing lactic acid accumulation after exercise.

[0035] Fifthly, the present invention provides the use of the maca and ginseng composition described in the first aspect or the oral preparation of maca and ginseng described in the second aspect in the preparation of a medicine for relieving physical fatigue and / or improving reproductive function.

[0036] In addition to relieving physical fatigue, the composition provided by this invention can also effectively increase serum testosterone levels. Testosterone plays a central role in spermatogenesis and libido regulation, maintaining reproductive system balance through the hypothalamic-pituitary-gonadal axis feedback mechanism. Sufficient testosterone levels ensure normal erectile function and fertility, while a deficiency may lead to oligospermia or sexual dysfunction.

[0037] Compared with existing technologies, the composition provided by this invention combines traditional Chinese medicine compatibility theory with modern pharmacological research results. It uses a variety of Chinese herbal extracts in a specific ratio, allowing the components to complement and enhance each other in their mechanism of action. This results in an overall efficacy of the composition that is far greater than the simple sum of the individual effects of the components. The raw materials selected for this invention are all nationally approved medicinal and edible substances or new food ingredients, ensuring high safety, low toxicity and side effects. Furthermore, the raw materials are widely available, possessing significant market value and development prospects. Detailed Implementation

[0038] The present invention provides the following embodiments to further illustrate various aspects of the invention. These embodiments are non-limiting and should not be construed as limiting any aspect of the invention. The scope of protection of the present invention is limited only by the claims. Various modifications and improvements can be made to various aspects of the present invention by those skilled in the art without departing from the scope of the claims, and these modifications and improvements also fall within the scope of protection of the present invention.

[0039] Additionally, it should be noted that, unless otherwise specified, all materials and reagents used in the following embodiments are commonly used in the art and can be obtained through conventional commercial means; all methods used are conventional methods known to those skilled in the art.

[0040] Example The following are ingredients used in the composition of the experimental examples: Maca powder is prepared by the following method: take raw maca purchased from Tongrentang, slice it, dry it, and pulverize it to obtain the product.

[0041] Cordyceps militaris powder is prepared by the following method: take Cordyceps militaris purchased from Tongrentang, dry it, and pulverize it to obtain the powder.

[0042] Cinnamon powder is prepared by the following method: take cinnamon purchased from Tongrentang, remove the bark, slice, dry, and pulverize it to obtain the powder.

[0043] Ginseng extract is prepared by the following method: Take ginseng purchased from Tongrentang, add water and boil to extract 3 times, 3 hours each time. The total amount of water used for extraction is equivalent to 20 times the weight of the Chinese herb. Collect the extract, centrifuge and filter, collect the filtrate, and spray dry to obtain the extract.

[0044] The wolfberry extract was prepared by the following method: wolfberries purchased from Tongrentang were boiled in water three times for three hours each time. The total amount of water used for extraction was equivalent to 20 times the weight of the wolfberry. The extract was collected, centrifuged and filtered, and the filtrate was collected and spray-dried to obtain the extract.

[0045] Mulberry extract was prepared by the following method: Take kudzu root purchased from Tongrentang, add water and boil to extract 3 times, 3 hours each time. The total amount of water used for extraction is equivalent to 20 times the mass of the Chinese herb. Collect the extract, centrifuge and filter, collect the filtrate, and spray dry to obtain the extract.

[0046] The extract of Polygonatum sibiricum was prepared by the following method: Polygonatum sibiricum purchased from Tongrentang was boiled in water and extracted three times, each time for 3 hours. The total amount of water used for extraction was equivalent to 20 times the mass of the Chinese herb. The extract was collected, centrifuged and filtered, the filtrate was collected, and spray-dried to obtain the final product.

[0047] The yam extract was prepared by the following method: Take Chinese yam purchased from Tongrentang, add water and boil to extract 3 times, 3 hours each time. The total amount of water used for extraction is equivalent to 20 times the mass of the Chinese yam. Collect the extract, centrifuge and filter, collect the filtrate, and spray dry to obtain the extract.

[0048] The extract of Cistanche deserticola was prepared by the following method: Cistanche deserticola (desert) purchased from Tongrentang was boiled in water and extracted three times, each time for 3 hours. The total amount of water used for extraction was equivalent to 20 times the mass of the Chinese herbal medicine. The extract was collected, centrifuged and filtered, the filtrate was collected, and spray-dried to obtain the extract.

[0049] The raspberry extract was prepared by the following method: Raspberries purchased from Tongrentang were boiled in water three times for three hours each time. The total amount of water used for extraction was equivalent to 20 times the weight of the raspberry. The extract was collected, centrifuged and filtered, and the filtrate was collected and spray-dried to obtain the extract.

[0050] Eucommia ulmoides leaf extract was prepared by the following method: Eucommia ulmoides leaves purchased from Tongrentang were boiled in water for 3 times, each time for 3 hours. The total amount of water used for extraction was equivalent to 20 times the mass of the Chinese herbal medicine. The extract was collected, centrifuged and filtered, and the filtrate was collected and spray-dried to obtain the extract.

[0051] The edible salt is natural sea salt (non-iodized) produced by China Salt Beijing-Tianjin-Hebei Salt Industry Co., Ltd.

[0052] Example 1 This embodiment provides a composition composed of the following components: 42 g of maca powder, 22 g of ginseng extract (component A), 23 g of wolfberry extract (component B), 25 g of cistanche extract (component C), 12 g of cinnamon powder, 6 g of eucommia leaf extract, and 3 g of edible salt.

[0053] Example 2 This embodiment provides a composition composed of the following components: 55 g of maca powder, 26 g of ginseng extract (component A), 27 g of wolfberry extract (component B), 28 g of cistanche extract (component C), 18 g of cinnamon powder, 7 g of eucommia leaf extract, and 5 g of edible salt.

[0054] Example 3 This embodiment provides a composition that differs from Embodiment 1 of the present invention only in that: Component A consists of 20 g of ginseng extract and 10 g of cordyceps powder.

[0055] Example 4 This embodiment provides a composition that differs from Embodiment 1 of the present invention only in that: component B consists of 6 g of wolfberry extract, 5 g of mulberry extract, 6 g of polygonatum extract and 4 g of yam extract.

[0056] Example 5 This embodiment provides a composition that differs from Embodiment 1 of the present invention only in that: component C consists of 14 g of Cistanche deserticola extract and 10 g of Raspberry extract.

[0057] Example 6 This embodiment provides a composition consisting of the following components: 50 g maca powder, 18 g ginseng extract, 8 g cordyceps powder, 6 g wolfberry extract, 5 g mulberry extract, 6 g polygonatum extract, 4 g yam extract, 12 g cistanche extract, 8 g raspberry extract, 12 g cinnamon powder, 6 g eucommia leaf extract, and 4 g edible salt.

[0058] Example 7 This embodiment provides a composition consisting of the following components: 55 g maca powder, 20 g ginseng extract, 10 g cordyceps powder, 8 g wolfberry extract, 7 g mulberry extract, 8 g polygonatum extract, 6 g yam extract, 14 g cistanche extract, 10 g raspberry extract, 15 g cinnamon powder, 7 g eucommia leaf extract, and 5 g edible salt.

[0059] Example 8 This embodiment provides a maca and ginseng compressed candy, prepared by a method including the following steps: 40 g of xylitol and 16 g of maltodextrin are passed through a 100-mesh sieve to obtain powder A; 10 g of the composition provided in Example 1 and 8 g of microcrystalline cellulose are passed through an 80-mesh sieve to obtain powder B; 0.7 g of magnesium stearate and 0.6 g of silicon dioxide are passed through a 60-mesh sieve to obtain powder C. Powder A and powder B are added to a three-dimensional mixer and mixed at 15 r / min for 10 min. After stopping the mixer, powder C is added and mixing continues for 5 min to obtain a homogeneous mixed powder; a high-speed shear granulator (paddle 300 r / min, cutter 1500 r / min) is turned on, and 50% ethanol is sprayed evenly, completing the process within 3 min, with an output particle size of 40 mesh; the product is dried in a fluidized bed at 55 ℃ to a moisture content of 3.0%, and then granulated through an 18-mesh vibrating sieve to obtain uniformly sized granules; finally, the product is compressed into tablets.

[0060] In the compressed candy provided in this embodiment, replacing the composition provided in Example 1 with the compositions provided in Examples 2 to 7 will yield the corresponding compressed candy.

[0061] Example 9 This embodiment provides a maca and ginseng solid beverage (granules), which is prepared by a method including the following steps: 32 g of the composition provided in Example 1, 11 g of xylitol and 15 g of maltodextrin are mixed and granulated by dry granulation to obtain the product.

[0062] In the solid beverage provided in this embodiment, replacing the composition provided in Example 1 with the compositions provided in Examples 2 to 7 can yield the corresponding granules.

[0063] Comparative Example 1 This comparative example provides a composition that differs from Example 1 of the present invention only in that the "medicine" therein does not contain component A, ginseng extract.

[0064] Comparative Example 2 This comparative example provides a composition that differs from Example 1 of the present invention only in that the "medicine" therein does not contain component B, wolfberry extract.

[0065] Comparative Example 3 This comparative example provides a composition that differs from Example 1 of the present invention only in that the "subject medicine" does not contain component C, Cistanche deserticola extract.

[0066] Experimental Example 1. Weighted swimming experiment Improved exercise endurance is the most direct manifestation of enhanced fatigue resistance, and the length of swimming time can reflect the degree of exercise fatigue in animals.

[0067] Ninety male BALB / c mice, SPF grade, weighing 18–22 g, were selected and randomly divided into 9 groups of 10 mice each. The test drug was administered via gavage at a dose of 12 mL / kg BW. The concentration of the test drug was further calculated from the recommended daily adult dose (2 g / d of the composition) to the mouse gavage dose. One group served as a blank control (gavage with distilled water), while the other eight experimental groups received the compositions provided in Examples 1, 3, 4, 5, 6, Comparative Examples 1, 2, and 3, respectively. The mice were administered the test drug for 30 consecutive days. Thirty minutes after the last administration of the test drug, the mice, with a lead weight of 5% of their body weight loaded at the base of their tails, were placed in a swimming tank at a depth of 30 cm and a water temperature of 25℃ ± 1.0℃. The time from the start of swimming until death was recorded, i.e., the weighted swimming time. The results are shown in Table 1.

[0068] Table 1: Results of the weighted swimming experiment (n=10)

[0069] The results show that the composition provided by the present invention can significantly increase the swimming time of mice under load, and the effect is better than that of each control group.

[0070] 2. Liver glycogen testing Ninety male BALB / c mice, SPF grade, weighing 18–22 g, were randomly divided into 9 groups of 10 mice each. The test drug was administered via gavage at a dose of 12 mL / kg BW. The concentration of the test drug was further calculated from the recommended daily adult dose (2 g / d of the composition) to the mouse gavage dose. One group served as a blank control (gavage with distilled water), while the other eight experimental groups received the compositions provided in Examples 1, 3, 4, 5, 6, Comparative Examples 1, 2, and 3, respectively. The test drugs were administered continuously for 30 days. Thirty minutes after the last administration, 100 mg of liver tissue from each group of mice (washed and blotted dry with filter paper) was weighed, homogenized in 8 mL of trichloroacetic acid, and centrifuged at 3000 r / min for 15 min. The supernatant was then used to determine the liver glycogen content. Anthrones can react with free sugars or polysaccharides, producing a blue-green solution with maximum absorption at 620 nm. By measuring the optical density, the anthrone method can be used to determine glycogen content. The results are shown in Table 2.

[0071] Table 2: Liver glycogen test results (n=10)

[0072] The results show that the composition provided by the present invention can significantly increase the content of liver glycogen in mouse liver tissue, and the effect is better than that of each comparison ratio.

[0073] 3. Post-exercise blood lactate measurement Ninety male BALB / c mice, SPF grade, weighing 18–22 g, were selected and randomly divided into 9 groups of 10 mice each. The test drug was administered via gavage at a dose of 12 mL / kg BW. The concentration of the test sample was further calculated from the recommended daily adult dose (2 g / d of the composition) for mice. One group served as a blank control (administered distilled water via gavage), while the other eight experimental groups received the compositions provided in Examples 1, 3, 4, 5, 6, Comparative Examples 1, 2, and 3, respectively. The test samples were administered continuously for 30 days. Thirty minutes after the last administration of the test sample, 20 μL of blood was collected from the inner canthus of each group of mice. The mice were then placed in a swimming tank at a depth of 30 cm and a water temperature of 30 ℃ and swam for 10 minutes. 20 μL of blood was collected at 0 min and 20 min after swimming, and the blood lactate content was measured at these three time points using a lactate analyzer. The area under the lactate curve after exercise was calculated. The calculation formula was: Area under the lactate curve = 0.5 × (lactate value before swimming + lactate value at 0 min after swimming) × 10 + 0.5 × (lactate value at 0 min after swimming + lactate value at 20 min after swimming) × 20. The results are shown in Table 3.

[0074] Table 3: Calculation results of area under the curve for mouse blood lactate (n=10)

[0075] The results show that the composition provided by the present invention can significantly reduce the blood lactate content after exercise, and the effect is better than that of the comparative examples.

[0076] 4. Serum testosterone level detection Ninety male BALB / c mice, SPF grade, weighing 18–22 g, were randomly divided into 9 groups of 10 mice each. The test drug was administered via gavage at a dose of 12 mL / kg BW. The concentration of the test sample was further calculated from the recommended daily adult dose (2 g / d of the composition) to the mouse gavage dose. One group served as a blank control (gavage with distilled water), while the other eight experimental groups received the compositions provided in Examples 1, 3, 4, 5, 6, Comparative Examples 1, 2, and 3, respectively. The test samples were administered continuously for 30 days. Thirty minutes after the last administration of the test sample, venous blood was collected from the mice, and serum testosterone levels were measured using a testosterone ELISA kit purchased from Shanghai Keabob Biotechnology Co., Ltd.

[0077] Table 4: Serum testosterone concentration (n=10)

[0078] The results show that the composition provided by the present invention can significantly increase serum testosterone levels, and the effect is better than that of the comparative ratios.

[0079] Based on the above animal experimental results, the composition provided by this invention can effectively increase the swimming time of mice under load, increase the content of liver glycogen in liver tissue, reduce lactic acid accumulation after exercise, and comprehensively relieve fatigue. In addition, it can also increase serum testosterone levels, showing potential to improve reproductive health. Among them, the composition provided in Example 6 has the best overall effect.

[0080] Unless otherwise stated, the technical terms used herein have the same meanings as commonly understood by one of ordinary skill in the art. This invention may also be practiced using any methods and materials similar to or equivalent to those described herein. While specific embodiments and preferred methods and materials are described herein, they do not impose any limitation on the invention.

Claims

1. A maca and ginseng composition, characterized in that, It contains the following ingredients by weight: 40-60 parts maca powder, 20-30 parts component A, 20-30 parts component B, 20-30 parts component C, 10-20 parts cinnamon powder, 5-8 parts eucommia leaf extract, and 3-6 parts edible salt. Component A contains ginseng extract; component B contains wolfberry extract; component C contains cistanche extract; The extracts are obtained by mixing Chinese herbal raw materials with water at a mass ratio of 1:10~20, followed by hot extraction, filtration of insoluble matter, collection of filtrate, and drying.

2. The composition according to claim 1, characterized in that, Component A is composed of ginseng extract and cordyceps militaris powder mixed in a mass ratio of (7~10):(3~6).

3. The composition according to claim 1, characterized in that, Component B is composed of wolfberry extract, mulberry extract, polygonatum extract and yam extract in a mass ratio of (6~8):(5~7):(6~8):(4~6).

4. The composition according to claim 1, characterized in that, Component C is a mixture of Cistanche deserticola extract and Raspberry extract in a mass ratio of (5~8):(3~6).

5. The composition according to any one of claims 1 to 4, characterized in that, It contains the following ingredients in parts by weight: 50-55 parts maca powder, 18-20 parts ginseng extract, 8-10 parts cordyceps powder, 6-8 parts wolfberry extract, 5-7 parts mulberry extract, 6-8 parts polygonatum extract, 4-6 parts yam extract, 12-14 parts cistanche extract, 8-10 parts raspberry extract, 12-15 parts cinnamon powder, 6-7 parts eucommia leaf extract, and 4-5 parts edible salt.

6. An oral preparation of maca and ginseng, characterized in that, The composition comprises the maca and ginseng extract as described in any one of claims 1 to 5, and excipients; The oral preparation is a tablet, preferably a regular tablet, lozenge, chewable tablet, dispersible tablet or effervescent tablet; Alternatively, the oral preparation may be a capsule, preferably a hard capsule or a soft capsule; Alternatively, the oral preparation is a granule, preferably a soluble granule, a suspension granule, or an effervescent granule; Alternatively, the oral preparation may be a powder; Alternatively, the oral preparation may be a paste; Alternatively, the oral preparation is a candy, preferably hard candy, hard filled candy, cream candy, gel candy, gum-based candy, aerated candy or compressed candy; Alternatively, the oral formulation may be a liquid formulation, preferably a syrup, oral liquid, or beverage.

7. The oral formulation according to claim 6, characterized in that, It is a compressed candy, and the sweetener used is xylitol.

8. A food product, characterized in that, The product comprises the maca and ginseng composition according to any one of claims 1 to 5 or the oral preparation of maca and ginseng according to claim 6 or 7.

9. The use of the maca and ginseng composition according to any one of claims 1 to 5 or the oral preparation of maca and ginseng according to claim 6 or 7 in the preparation of health food for relieving physical fatigue.

10. The use of the maca and ginseng composition according to any one of claims 1 to 5 or the oral preparation of maca and ginseng according to claim 6 or 7 in the preparation of a medicine for relieving physical fatigue and / or improving reproductive capacity.

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