Biological cell freezing visualization system and method based on metal woven belt
By using metal braided straps to conduct cold energy and controlling the lifting slide, combined with a vacuum pump and thermocouple detection, the problem of decreased imaging clarity during biological cell freezing was solved, achieving rapid cooling and precise control, and improving the imaging effect of the device.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SHANGHAI SQBQ BIOTECHNOLOGY CO LTD
- Filing Date
- 2024-04-19
- Publication Date
- 2026-05-26
AI Technical Summary
In existing visualization systems for biological cell freezing processes, the gamete visualization environment chamber requires extremely high precision in its stopping position, which leads to a decrease in image clarity.
The system uses metal braided straps to conduct cold energy, and controls the contact and separation between the constant-low temperature copper platform and the metal braided straps through a lifting slide. Combined with the temperature detection of a vacuum pump and thermocouples, the freezing process is precisely controlled.
It enables rapid cooling of biological cell samples, improves imaging clarity and device durability, and avoids unclear imaging caused by positional deviation during freezing.
Smart Images
Figure CN122074476A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of biotechnology, and in particular to a biological cell freezing visualization system and method based on metal braided tape. Background Technology
[0002] Rapid cell freezing is a research hotspot in the biological field, and vitrification is a rapid freezing technique. Microscopic video recording systems for visualizing the freezing process of biological cells are of great value, enabling rapid cooling of samples through intermittent contact with a low-temperature cold surface, and providing a system for visualizing the rapid freezing process.
[0003] Existing microscopic recording systems for visualizing biological cell freezing processes, such as CN 110057821 A, disclose a cryogenic microscopic imaging system for observing the rapid freeze-thaw process of human gametes, involving the cryopreservation of human gametes. The system's cold-conducting freezing and cooling module moves the gamete visual environment chamber downwards so that its surface is in close contact with the liquid nitrogen heat sink chamber. This requires extremely high precision in the stopping position of the gamete visual environment chamber, as any deviation in the stopping position will result in a decrease in imaging clarity. Summary of the Invention
[0004] This invention provides a biological cell cryopreservation visualization system and method based on metal braided straps, which solves the defects in the prior art where the cooling of the gamete visual environment chamber requires extremely high accuracy in stopping the gamete visual environment chamber, and deviations in stopping position will cause a decrease in image clarity.
[0005] On one hand, the present invention provides a biological cell cryopreservation visualization system based on metal braided straps, including a base plate. A support platform, a cryogenic liquid tank, and an observation component are fixedly connected to the upper surface of the base plate. A lifting slide is fixedly connected to the bottom surface of the support platform. A constant-temperature copper stage is fixedly connected to the upper surface of the lifting slide. The constant-temperature copper stage is connected to the cryogenic liquid tank through an infusion tubing. A braided strap fixing bracket and a sample placement platform are fixedly connected to the top surface of the support platform. One end of the metal braided strap is fixedly connected to the lower surface of the braided strap fixing bracket, and the other end of the metal braided strap is fixedly connected to the lower surface of the sample placement platform. A cover plate is placed on top of the sample placement platform. The observation component is used to capture images of the sample placement platform.
[0006] Preferably, the observation assembly includes a main support, which is fixedly connected to the upper surface of the base plate. A video recorder is mounted on the main support, and a biological microscope is mounted on the video recorder. The biological microscope is used to magnify the image.
[0007] Preferably, the constant low temperature copper platform is equipped with a liquid pipeline inside. The inlet end of the liquid pipeline is connected to the outlet of the infusion hose. The inlet of the infusion hose extends into the bottom of the deep low temperature liquid tank. The outlet end of the liquid pipeline is equipped with an air pump interface for connecting a vacuum pump.
[0008] Preferably, the infusion tubing is wrapped with insulating cotton.
[0009] Preferably, the metal braided strip is made of pure copper or pure silver, and its outer surface is wrapped with heat-insulating cotton. One end of the metal braided strip installed on the lower surface of the sample placement platform has an optical path through hole. The center of the cover plate is inlaid with high-transparency quartz glass, and the lower surface of the cover plate is provided with an annular groove, in which a high-insulation sealing gasket is inlaid.
[0010] Preferably, the sample placement platform is made of pure copper or pure silver, with a glass slide placement groove on the upper surface and an optical path through hole in the center. A thermocouple is embedded inside the sample placement platform and is positioned on the bottom surface of the sample placement platform near the center.
[0011] This invention also provides a method for visualizing biological cell cryopreservation based on metal braided tape, used to control the aforementioned biological cell cryopreservation visualization system based on metal braided tape, comprising: Step S1: Connect a vacuum pump to the air pump interface, start the vacuum pump, and pump the cryogenic liquid in the cryogenic liquid tank into the constant temperature copper platform through the infusion hose for evaporation. Step S2: Open the cover, place the glass slide to be processed into the glass slide placement groove of the sample placement platform, and close the cover. Step S3: After the temperature of the constant low temperature copper stage reaches the specified temperature, turn on the video recorder and control the lifting slide to rise so that the upper surface of the constant low temperature copper stage comes into contact with the metal braided belt. The cold energy is transferred through the metal braided belt to cool the glass slide to be processed. Step S4: After the upper surface of the constant low temperature copper platform is in contact with the metal braided strip for a certain period of time, the lifting slide is controlled to descend, so that the upper surface of the constant low temperature copper platform separates from the metal braided strip.
[0012] Preferably, step S4 includes: Step S41: When the thermocouple detection result reaches the preset start-up temperature, start timing; Step S42: Calculate the duration of continued contact between the upper surface of the constant low temperature copper platform and the metal braided strip; Step S43: After the timer reaches the duration of continued contact between the upper surface of the constant low temperature copper platform and the metal braided strip, control the lifting slide to descend.
[0013] Preferably, the duration of continued contact between the upper surface of the constant low temperature copper platform and the metal braided strip is calculated based on the following formula; ; Where t is the duration of continued contact between the upper surface of the constant-temperature copper platform and the metal braided strip; ln is a logarithmic function with base e; The preset target temperature; The preset start-up temperature; The ambient temperature inside the support platform; The unit of time is K; K is the thermal conductivity coefficient. D is the contact area between the metal braided strip and the lower surface of the sample placement platform; D is the thickness of the bottom surface of the sample placement platform. The Stefan-Boltzmann constant is given. ; Emissivity of the outer surface of the sample placement platform; The surface emissivity of the metal braided tape; C represents the outer surface area of the sample placement platform; C represents the specific heat capacity of the sample placement platform material; and M represents the mass of the sample placement platform.
[0014] Preferred options also include: Step S421: After timing for a period of time, calculate the verification temperature; ; in, Let i be the check temperature at time i; Let be the time elapsed from the start of timing to time i; C is the specific heat capacity of the sample placement platform material; M is the mass of the sample placement platform. Step S422: Obtain the actual detected temperature of the thermocouple at the current moment; Step S423: Calculate the temperature deviation. If the temperature deviation is greater than the danger threshold, an alarm is triggered and the corrected contact time is calculated. The upper surface of the constant low temperature copper platform is controlled to continue to contact the metal braided strip until the corrected contact time is achieved and then the contact is separated. ; Where Q represents the temperature deviation; Let i be the actual temperature detected by the thermocouple at time i; This is the preset calculation reference temperature value; ; Where t is the duration of continued contact between the upper surface of the constant-temperature copper platform and the metal braided strip; ln is a logarithmic function with base e; The preset target temperature; Let i be the actual temperature detected by the thermocouple at time i; The temperature at which the metal braided tape reaches a steady state; The ambient temperature inside the support platform; The unit of time is K; K is the thermal conductivity coefficient. D is the contact area between the metal braided strip and the lower surface of the sample placement platform; D is the thickness of the bottom surface of the sample placement platform. The Stefan-Boltzmann constant is given. ; Emissivity of the outer surface of the sample placement platform; The surface emissivity of the metal braided tape; C represents the outer surface area of the sample placement platform; C represents the specific heat capacity of the sample placement platform material; and M represents the mass of the sample placement platform.
[0015] Compared with the prior art, the present invention has the following beneficial effects: This invention conducts cold energy through a metal braided belt. Both the metal braided belt and the sample placement platform are made of pure copper or pure silver, resulting in extremely high and fast cold conduction efficiency, enabling rapid cooling of biological cell samples. The transfer of cold energy through the metal braided belt reduces the requirements for the device's motion precision, thus improving the device's durability. The fixed position of the sample placement platform ensures clear imaging of the observation components. Attached Figure Description
[0016] To more clearly illustrate the technical solutions in this invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.
[0017] Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is a schematic diagram of the observation component of the present invention.
[0018] Figure label: 1. Base plate; 2. Support platform; 3. Cryogenic liquid tank; 4. Lifting slide; 5. Constant temperature copper platform; 6. Infusion tubing; 7. Metal braided strap; 8. Braided strap fixing bracket; 9. Sample placement platform; 10. Cover plate; 11. Main support; 12. Video recorder; 13. Biological microscope; 14. Air pump interface. Detailed Implementation
[0019] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.
[0020] Furthermore, in this invention, the use of terms such as "first" and "second" is for descriptive purposes only and does not specifically refer to any order or sequence, nor is it intended to limit the invention. They are merely used to distinguish components or operations described using the same technical terms and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the technical solutions and features of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. If a combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this invention.
[0021] Example 1 This invention provides a visualization system for biological cell cryopreservation based on metal braided tape, such as... Figure 1-2 As shown, the system includes a base plate 1. A support platform 2, a cryogenic liquid tank 3, and an observation component are fixedly connected to the upper surface of the base plate 1. A lifting slide 4 is fixedly connected to the bottom surface of the support platform 2. A constant-temperature copper platform 5 is fixedly connected to the upper end of the lifting slide 4. The constant-temperature copper platform 5 is connected to the cryogenic liquid tank 3 through an infusion hose 6. A braided strap fixing bracket 8 and a sample placement platform 9 are fixedly connected to the top surface of the support platform 2. One end of the metal braided strap 7 is fixedly connected to the lower surface of the braided strap fixing bracket 8, and the other end of the metal braided strap 7 is fixedly connected to the lower surface of the sample placement platform 9. A cover plate 10 is placed on top of the sample placement platform 9. The observation component is used to capture images of the sample placement platform 9.
[0022] Preferably, the observation assembly includes a main support 11, which is fixedly connected to the upper surface of the base plate 1. A video recorder 12 is mounted on the main support 11, and a biological microscope 13 is mounted on the video recorder 12. The biological microscope 13 is used to magnify the image.
[0023] Preferably, the constant low temperature copper platform 5 is equipped with a liquid pipeline inside. The inlet end of the liquid pipeline is connected to the outlet of the infusion hose 6. The inlet of the infusion hose 6 extends into the bottom of the deep low temperature liquid tank 3. The outlet end of the liquid pipeline is equipped with an air pump interface 14, which is used to connect a vacuum pump.
[0024] Preferably, the infusion tubing 6 is wrapped with insulating cotton.
[0025] Preferably, the metal braided strip 7 is made of pure copper or pure silver, and its outer surface is wrapped with heat-insulating cotton. One end of the metal braided strip 7 installed on the lower surface of the sample placement platform 9 has an optical path through hole. The center of the cover plate 10 is inlaid with high-transparency quartz glass, and the lower surface of the cover plate 10 is provided with an annular groove, in which a high-insulation sealing gasket is inlaid.
[0026] Preferably, the sample placement platform 9 is made of pure copper or pure silver, with a glass slide placement groove on the upper surface and an optical path through hole in the center. A thermocouple is embedded inside the sample placement platform 9 and is located on the bottom surface of the sample placement platform 9 near the center.
[0027] The beneficial effects of the above technical solution are as follows: 1. The present invention conducts cold energy through a metal braided strip 7. Both the metal braided strip 7 and the sample placement platform 9 are made of pure copper or pure silver, which has extremely high cooling efficiency and speed, enabling biological cell samples to cool down rapidly.
[0028] 2. This invention uses the lifting and lowering control of the lifting slide 4 to raise the constant-low temperature copper platform 5 into close contact with the metal braided belt 7 for heat conduction. The cold energy is transferred to the biological cell sample on the glass slide in the groove of the cold platform and rapidly cools it down. This control method allows the sample to remain unaffected by low temperature in standby mode, while enabling rapid cooling when necessary. Furthermore, the fixed position of the sample placement platform 9 ensures clear imaging of the observation components.
[0029] 3. This invention controls the start and stop of the vacuum pump, cools the constant low temperature copper platform 5 by the flow of deep cryogenic liquid in the liquid pipeline, and then transfers the cooling energy through the metal braided belt 7, thus avoiding the observation optical path and preventing fogging at the observation position from affecting the clarity of the observation.
[0030] Example 2 Based on Example 1, this invention also discloses a method for visualizing biological cell cryopreservation based on metal braided strips, used to control a biological cell cryopreservation visualization system based on metal braided strips as described in Example 1, comprising: Step S1: Connect a vacuum pump to the air pump interface 14, start the vacuum pump, and pump the cryogenic liquid in the cryogenic liquid tank 3 into the constant low temperature copper platform 5 through the infusion hose 6 for evaporation. Step S2: Open the cover plate 10, place the glass slide to be processed into the glass slide placement groove of the sample placement platform 9, and close the cover plate 10. Step S3: After the temperature of the constant low temperature copper stage 5 reaches the specified temperature, turn on the video recorder 12 and control the lifting slide 4 to rise so that the upper surface of the constant low temperature copper stage 5 comes into contact with the metal braided belt 7. The cold energy is transferred through the metal braided belt 7 to cool down the glass slide to be processed. In step S4, after the upper surface of the constant low temperature copper platform 5 has been in contact with the metal braided belt 7 for a certain period of time, the lifting slide 4 is controlled to descend, so that the upper surface of the constant low temperature copper platform 5 is separated from the metal braided belt 7.
[0031] Preferably, step S4 includes: Step S41: When the thermocouple detection result reaches the preset start-up temperature, start timing; Step S42: Calculate the duration of continued contact between the upper surface of the constant low temperature copper platform 5 and the metal braided strip 7; Step S43: After timing reaches the duration of continued contact between the upper surface of the constant low temperature copper platform 5 and the metal braided belt 7, control the lifting slide 4 to descend.
[0032] The beneficial effects of the above technical solution are as follows: A vacuum pump draws cryogenic liquid into the liquid pipeline to cool the constant-temperature copper platform 5. The cold energy is then transferred through the metal braided belt 7, bypassing the observation optical path and preventing fogging at the observation position from affecting the clarity of the observation. The constant-temperature copper platform 5 is first cooled to the specified temperature before contacting the metal braided belt 7, avoiding unstable cold energy transfer during the cooling process of the constant-temperature copper platform 5, which would affect the cooling rate of the freezing process of biological cells and help improve the freezing effect of biological cells. The upper surface of the constant-temperature copper platform 5 automatically separates from the metal braided belt 7 after a certain period of contact, avoiding excessive cooling due to excessive contact time, which could cause irreversible damage to biological cells and further ensure the freezing effect of biological cells.
[0033] By starting the timing only after the thermocouple's detection result reaches the start-up temperature, the unstable cold energy transfer in the initial stage avoids large fluctuations in contact time, which would affect the accuracy of time control. By calculating the contact time between the upper surface of the constant low temperature copper platform 5 and the metal braided strip 7 from the time the thermocouple's detection result reaches the start-up temperature until the thermocouple's detection result reaches the target temperature, the contact time between the upper surface of the constant low temperature copper platform 5 and the metal braided strip 7 is precisely controlled, which is beneficial to improving the freezing effect on biological cells.
[0034] Example 3 Based on Example 2, the duration of continued contact between the upper surface of the constant low temperature copper platform 5 and the metal braided strip 7 is calculated based on the following formula; ; Where t is the duration of continued contact between the upper surface of the constant low temperature copper platform 5 and the metal braided strip 7; ln is a logarithmic function with base e; The preset target temperature; The preset start-up temperature; The ambient temperature inside the support platform 2; The unit of time is K; K is the thermal conductivity coefficient. 7 is the contact area between the metal braided strip 7 and the lower surface of the sample placement platform 9; D is the bottom thickness of the sample placement platform 9. The Stefan-Boltzmann constant is given. ; Emissivity of the outer surface of sample placement platform 9; The surface emissivity of the metal braided tape 7; C is the outer surface area of the sample placement platform 9; C is the specific heat capacity of the material of the sample placement platform 9; M is the mass of the sample placement platform 9.
[0035] The beneficial effects of the above technical solution are as follows: pass and The received cold and lost heat per unit time of the sample placement platform 9 were calculated respectively, thus providing data support for calculating the contact duration. By considering the temperature difference between the start-up temperature and the target temperature and the physical properties of the sample placement platform, the length of time that the constant low temperature copper platform 5 needs to be in contact with the metal braided belt 7 was calculated, thereby achieving precise control of the contact time, further achieving precise control of the temperature of the sample placement platform 9, and ultimately achieving the purpose of improving the freezing effect on biological cells.
[0036] Example 4 Based on Example 2, it also includes: Step S421: After timing for a period of time, calculate the verification temperature; ; in, Let i be the check temperature at time i; Let be the time elapsed from the start of timing to time i; C is the specific heat capacity of the material of sample placement platform 9; M is the mass of sample placement platform 9. Step S422: Obtain the actual detected temperature of the thermocouple at the current moment; Step S423: Calculate the temperature deviation. If the temperature deviation is greater than the danger threshold, an alarm is triggered and the corrected contact time is calculated. The upper surface of the constant low temperature copper platform 5 is controlled to continue to contact the metal braided belt 7 for the corrected contact time before separation. ; Where Q represents the temperature deviation; Let i be the actual temperature detected by the thermocouple at time i; This is the preset calculation reference temperature value; ; Where t is the duration of continued contact between the upper surface of the constant low temperature copper platform 5 and the metal braided strip 7; ln is a logarithmic function with base e; The preset target temperature; Let i be the actual temperature detected by the thermocouple at time i; The temperature at which the metal braided tape 7 reaches a steady state; The ambient temperature inside the support platform 2; The unit of time is K; K is the thermal conductivity coefficient. 7 is the contact area between the metal braided strip 7 and the lower surface of the sample placement platform 9; D is the bottom thickness of the sample placement platform 9. The Stefan-Boltzmann constant is given. ; Emissivity of the outer surface of sample placement platform 9; The surface emissivity of the metal braided tape 7; C is the outer surface area of the sample placement platform 9; C is the specific heat capacity of the material of the sample placement platform 9; M is the mass of the sample placement platform 9.
[0037] The beneficial effects of the above technical solution are as follows: By calculating the verification temperature after a period of time from the start of timing, and then obtaining the actual detection temperature of the thermocouple at the current moment, the temperature deviation is calculated to determine whether the cooling process deviates significantly from the expected situation. If no significant deviation occurs, the plan remains unchanged. If a significant deviation occurs, the corrected contact time is calculated, and the contact time between the upper surface of the constant low temperature copper platform 5 and the metal braided belt 7 is adjusted to ensure that the sample placement platform 9 can reach the target temperature when the upper surface of the constant low temperature copper platform 5 separates from the metal braided belt 7, thus ensuring the freezing effect on biological cells.
[0038] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims
1. A biological cell cryopreservation visualization system based on metal braided strips, characterized in that, The system includes a base plate (1), a support platform (2), a cryogenic liquid tank (3), and an observation component, which are fixedly connected to the upper surface of the base plate (1). A lifting slide (4) is fixedly connected to the bottom surface of the support platform (2), and a constant low temperature copper platform (5) is fixedly connected to the upper end of the lifting slide (4). The constant low temperature copper platform (5) is connected to the cryogenic liquid tank (3) through an infusion hose (6). A braided strap fixing bracket (8) and a sample placement platform (9) are fixedly connected to the top surface of the support platform (2). One end of the metal braided strap (7) is fixedly connected to the lower surface of the braided strap fixing bracket (8), and the other end of the metal braided strap (7) is fixedly connected to the lower surface of the sample placement platform (9). A cover plate (10) is placed on top of the sample placement platform (9). The observation component is used to capture images inside the sample placement platform (9).
2. The biological cell cryopreservation visualization system based on metal braided strips according to claim 1, characterized in that, The observation assembly includes a main support (11), which is fixedly connected to the upper surface of the base plate (1). A video recorder (12) is mounted on the main support (11), and a biological microscope (13) is mounted on the video recorder (12). The biological microscope (13) is used to magnify the image.
3. The biological cell cryopreservation visualization system based on metal braided strips according to claim 1, characterized in that, The constant low temperature copper platform (5) is equipped with a liquid pipeline. The inlet end of the liquid pipeline is connected to the outlet of the infusion hose (6). The inlet of the infusion hose (6) extends into the bottom of the deep low temperature liquid tank (3). The outlet end of the liquid pipeline is equipped with an air pump interface (14). The air pump interface (14) is used to connect a vacuum pump.
4. The biological cell cryopreservation visualization system based on metal braided strips according to claim 1, characterized in that, The infusion tubing (6) is wrapped with insulating cotton.
5. The biological cell cryopreservation visualization system based on metal braided strips according to claim 1, characterized in that, The metal braided strip (7) is made of pure copper or pure silver and is wrapped with heat-insulating cotton on its outer surface. The metal braided strip (7) is installed on one end of the sample placement platform (9) with a light path through hole. The cover plate (10) has a high-transparency quartz glass embedded in the center and an annular groove on the lower surface of the cover plate (10). A high-insulation sealing gasket is embedded in the groove.
6. The biological cell cryopreservation visualization system based on metal braided tape according to claim 1, characterized in that, The sample placement platform (9) is made of pure copper or pure silver. The upper surface has a glass slide placement groove, and the center has a light path through hole. A thermocouple is embedded inside the sample placement platform (9) and is located on the bottom surface of the sample placement platform (9) near the center.
7. A method for visualizing biological cell cryopreservation based on metal braided strips, used to control a biological cell cryopreservation visualization system based on metal braided strips as described in any one of claims 1-6, characterized in that, include: Step S1: Connect a vacuum pump to the air pump interface (14), start the vacuum pump, and pump the cryogenic liquid in the cryogenic liquid tank (3) into the constant low temperature copper platform (5) along the infusion hose (6) for evaporation. Step S2: Open the cover plate (10), place the glass slide to be processed into the glass slide placement groove of the sample placement platform (9), and close the cover plate (10); Step S3: After the temperature of the constant low temperature copper stage (5) reaches the specified temperature, turn on the video recorder (12) and control the lifting slide (4) to rise so that the upper surface of the constant low temperature copper stage (5) comes into contact with the metal braided belt (7). The cold energy is transferred through the metal braided belt (7) to cool down the glass slide to be processed. Step S4: After the upper surface of the constant low temperature copper platform (5) has been in contact with the metal braided strip (7) for a certain period of time, the lifting slide (4) is controlled to descend, so that the upper surface of the constant low temperature copper platform (5) is separated from the metal braided strip (7).
8. The method for visualizing biological cell cryopreservation based on metal braided strips according to claim 7, characterized in that, Step S4 includes: Step S41: When the temperature detected by the thermocouple reaches the preset start-up temperature, start timing; Step S42, calculate the duration of continued contact between the upper surface of the constant low temperature copper platform (5) and the metal braided strip (7); Step S43: After timing the duration of continued contact between the upper surface of the constant low temperature copper platform (5) and the metal braided strip (7), control the lifting slide (4) to descend.
9. The method for visualizing biological cell freezing based on metal braided strips according to claim 8, characterized in that, The duration of continued contact between the upper surface of the constant low temperature copper platform (5) and the metal braided strip (7) is calculated based on the following formula; ； Where t is the duration of continued contact between the upper surface of the constant low temperature copper platform (5) and the metal braided strip (7); ln is a logarithmic function with base e; The preset target temperature; The preset start-up temperature; The temperature at which the metal braided tape (7) reaches steady state; The ambient temperature inside the support platform (2); The unit of time is K; K is the thermal conductivity coefficient. D is the contact area between the metal braided strip (7) and the lower surface of the sample placement platform (9); D is the bottom thickness of the sample placement platform (9); The Stefan-Boltzmann constant, ; Emissivity of the outer surface of the sample placement platform (9); The surface emissivity of the metal braided strip (7); C is the outer surface area of the sample placement platform (9); C is the specific heat capacity of the material of the sample placement platform (9); M is the mass of the sample placement platform (9).
10. The method for visualizing biological cell freezing based on metal braided tape according to claim 8, characterized in that, Also includes: Step S421: After timing for a period of time, calculate the verification temperature; ； in, Let i be the check temperature at time i; C is the time elapsed from the start of timing to time i; C is the specific heat capacity of the material of the sample placement platform (9); M is the mass of the sample placement platform (9); Step S422: Obtain the actual detected temperature of the thermocouple at the current moment; Step S423: Calculate the temperature deviation. If the temperature deviation is greater than the danger threshold, an alarm is triggered and the corrected contact time is calculated. The upper surface of the constant low temperature copper platform (5) is controlled to continue to contact the metal braided strip (7) and then separate after the corrected contact time. ； Where Q represents the temperature deviation; Let i be the actual temperature detected by the thermocouple at time i; This is the preset calculation reference temperature value; ； Where t is the duration of continued contact between the upper surface of the constant low temperature copper platform (5) and the metal braided strip (7); ln is a logarithmic function with base e; The preset target temperature; The temperature at which the metal braided tape (7) reaches steady state; The ambient temperature inside the support platform (2); The unit of time is K; K is the thermal conductivity coefficient. D is the contact area between the metal braided strip (7) and the lower surface of the sample placement platform (9); D is the bottom thickness of the sample placement platform (9); The Stefan-Boltzmann constant, ; Emissivity of the outer surface of the sample placement platform (9); The surface emissivity of the metal braided strip (7); C is the outer surface area of the sample placement platform (9); C is the specific heat capacity of the material of the sample placement platform (9); M is the mass of the sample placement platform (9).