A shampoo conditioning composition containing a palm tree extract and a method for making the same

Abstract

This invention relates to a shampoo and conditioner composition containing extract of *Phyllostachys edulis* and its preparation method. The shampoo and conditioner composition of this invention includes extract of *Phyllostachys edulis*, extract of patchouli leaf, surfactant, moisturizer, hair conditioner, skin conditioner, preservative, fragrance, pH adjuster, and deionized water. By rationally constructing the system architecture of the shampoo and conditioner composition, using extract of *Phyllostachys edulis* as the main active ingredient, combined with patchouli leaf extract, the combination of the two can effectively improve the oil control and dandruff removal effect of the shampoo and conditioner composition. The resulting shampoo and conditioner composition has a moderate scalp cleansing and conditioning effect, is safe and gentle, and has a good foaming feel. After use, the combability of dry and wet hair is significantly improved, which can delicately nourish hair strands, smooth damaged hair cuticles, restore the hair's moisture and texture, and improve frizz and tangles.

Description

Technical Field

[0001] This invention belongs to the field of daily chemical products, specifically relating to a shampoo and conditioner composition containing extracts of the red algae *Phyllostachys edulis* and its preparation method. Background Technology

[0002] As people's living standards continue to improve, the demand for daily chemical products is evolving towards higher quality and efficiency. In the shampoo and hair care market, this trend is reflected in the shift in consumers' core demands: from meeting basic cleaning and care functions to focusing on product user experience and proven efficacy. For the new generation of consumers who pay particular attention to their personal image, the ideal hair quality is specifically manifested as healthy, shiny, well-defined, non-greasy hair that feels smooth to the touch and free of tangles or frizz. This has become a key benchmark for defining product value.

[0003] Red algae extract is used in cosmetics as a skin conditioning agent, whitening agent, and antioxidant. Red algae grows on the northern coasts of the Atlantic and Pacific Oceans and is rich in minerals, vitamins, trace elements, proteins, and polyphenols, providing nutrients to the skin, promoting skin metabolism, and making the skin delicate and smooth. Red algae extract can also scavenge hydroxyl free radicals, helping the skin fight oxidation, protecting the skin, and delaying aging. For example, patent number CN106963692B, "A Cosmetic Composition Containing Fucus vesiculosus Extract," discloses a cosmetic composition containing both Fucus vesiculosus extract and red algae extract, which has good whitening effect, significant fine line removal effect, good skin firmness, and good moisturizing effect. Patent number CN110897943A, "A Whitening Mask and Its Preparation Method," discloses a cosmetic composition combining red algae extract with synthetic whitening ingredients, which can achieve whitening and spot removal, enhance skin elasticity, and delay skin aging.

[0004] In summary, the current application of *Rhizophora rubiginosa* extract in the cosmetics industry is highly focused on skincare, particularly in anti-oxidation, anti-aging, and whitening. However, its application in hair care products is not observed. The applicant discovered that, in addition to widely reported emodin, chrysophanol, and polyphenols, *Rhizophora rubiginosa* extract also contains cellulose, plant proteins, and complex amino acids. These proteins and amino acids, due to their structural homology with hair keratin, readily adhere to hair strands, thus exhibiting excellent conditioning effects in repairing hair damage and providing long-lasting hydration. Therefore, a shampoo and conditioner composition containing *Rhizophora rubiginosa* extract has been developed. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a shampoo and conditioner composition containing extract of *Phyllostachys edulis* and its preparation method. The shampoo and conditioner composition of the present invention, through the rational construction of the shampoo and conditioner composition system architecture, uses *Phyllostachys edulis* extract as the core active ingredient, and synergistically combines it with patchouli leaf extract to effectively improve the oil control and dandruff removal effect of the shampoo and conditioner composition. The resulting shampoo and conditioner composition has a moderate scalp cleansing and conditioning effect, is safe and gentle, and has a good foaming feel. After use, the combability of dry and wet hair is significantly improved, which can delicately nourish hair strands, smooth damaged hair cuticles, restore the hair's shine, and improve frizz and tangles.

[0006] The technical solution adopted by the present invention to solve the above problems is as follows: On one hand, the present invention provides a shampoo and conditioner composition containing extract of the red algae *Callicarpa arvensis*, comprising the following raw materials by mass percentage: 0.1%–10% *Callicarpa arvensis* extract; 0.1%–0.5% *Pogostemon cablin* leaf extract; 14%–25% surfactant; 0.3%–5% moisturizer; 0.1%–1% hair conditioner; 0.5%–1% skin conditioner; 0.5%–1% preservative; 0.5%–1% fragrance; 0.01%–0.2% pH adjuster; and the remainder deionized water to bring the total to 100%.

[0007] In the aforementioned technical solution, the extract of *Rhododendron simsii* contains emodin, chrysophanol, polyphenols, plant proteins, and complex amino acids. The abundant plant proteins and complex amino acids effectively repair damaged hair cuticles, improve frizz and tangles, and give hair a glossy, hydrated texture. The emodin, chrysophanol, and polyphenols in the extract have multiple benefits for hair and scalp, primarily including promoting hair growth, improving scalp health, and providing antioxidant protection. These components also have antibacterial and bacteriostatic effects, inhibiting various bacteria such as Staphylococcus aureus and Streptococcus, and are also effective against anaerobic bacteria. The strong inhibitory effect helps improve scalp inflammation and infection problems. These ingredients can astringe the skin and regulate sebum secretion, which can improve oily scalp and seborrheic alopecia. These active ingredients can improve scalp permeability, promote the absorption of other effective ingredients, and enhance the overall effect of hair care products. Emodin and rhein can stimulate hair follicle growth, promote scalp blood circulation, and provide sufficient nutritional support to hair follicles, thereby promoting hair growth and regeneration. Polyphenols have strong antioxidant capabilities, which can scavenge free radicals, reduce oxidative stress damage to hair follicles, and delay the hair aging process. In addition, the emodin, rhein and other components in the extract of the red algae palm leaf tree can effectively inhibit the growth and reproduction of Malassezia, and regulate the number of Malassezia from the root by removing excess sebum—an important condition for Malassezia to grow. This reduces the production of dandruff-related peroxides, thereby blocking the dandruff formation pathway and achieving a comprehensive care effect of dandruff removal and itch relief.

[0008] In addition, patchouli leaf extract contains abundant active ingredients, mainly including sesquiterpenoids such as patchouli alcohol, patchouli ketone, and β-patchoulene, as well as flavonoids and phenolic acids. These active substances endow patchouli leaf extract with a variety of biological activities, including antibacterial and anti-inflammatory, soothing and repairing, oil-controlling and astringent, antioxidant, and scalp barrier repair effects. Among them, patchouli alcohol has anti-inflammatory effects, can inhibit the overactivity of TRPV1 receptors, reduce discomfort symptoms such as scalp burning and stinging, and has a soothing effect on sensitive scalps; patchouli ketone has a significant inhibitory effect on common scalp pathogens such as Staphylococcus aureus and Candida albicans, and can effectively improve scalp itching, dandruff, and other problems; the antioxidant components in patchouli leaf extract can scavenge free radicals, protect scalp cells, promote the repair of scalp barrier function, regulate sebum secretion, tighten pores, improve oily scalp problems, and make hair more refreshing.

[0009] The applicant unexpectedly discovered that by using the extract of the red algae *Cinnamomum camphora* as the core active ingredient, in synergistic combination with patchouli leaf extract, the two ingredients can effectively enhance the oil-control and dandruff-removing effects of the shampoo and conditioner composition. The resulting shampoo and conditioner composition has a moderate scalp cleansing and conditioning effect, is safe and gentle, and provides a pleasant lather. After use, the combability of dry and wet hair is significantly improved, resulting in delicate hair care, smoothing damaged hair cuticles, restoring the hair's shine, and improving frizz and tangles. The pH value of the shampoo and conditioner composition of this invention is controlled between 5.5 and 6.0, which is close to the slightly acidic environment of the scalp, helping to maintain the stability of the scalp barrier, preventing damage to the surface sebum film, and reducing product irritation.

[0010] The extract of *Cymbidium goeringii* used in the shampoo and conditioner composition of the present invention can be obtained commercially or by self-production. Preferably, the extract is obtained by the following extraction method, which includes the following steps: S1. Raw material pretreatment: Take fresh red algae, wash the whole plant of Red Algae Palm Leaf Tree, cut it into small sections, vacuum freeze dry it, and then grind it into powder; S2. Low-temperature enzymatic hydrolysis: The powder is transferred into a low-temperature reaction vessel, protected by an inert gas, and a citrate-sodium citrate buffer solution is added. Then, a compound enzyme is added, and enzymatic hydrolysis is carried out at low temperature with the assistance of low-frequency ultrasound. The obtained enzymatic hydrolysate is filtered to obtain the enzymatic hydrolysis residue and the enzymatic hydrolysis filtrate. In the above technical solution, the role of low-frequency ultrasound-assisted enzymatic hydrolysis is to promote the contact between the complex enzyme and the cell wall through cavitation effect, thereby promoting the enzymatic hydrolysis process.

[0011] S3. Combined ultrasonic-cold plasma extraction: The enzymatically hydrolyzed filter residue is added to a 30-40% ethanol-water mixed solvent containing vitamin C and subjected to cold plasma treatment. Then, ultrasonic extraction is started, followed by filtration. The obtained filter residue is added to a 30-40% ethanol-water mixed solvent containing vitamin C, and ultrasonic extraction and filtration are repeated 2-3 times to obtain the extraction filter residue and filtrate. The multiple filtrates are combined to obtain the extraction filtrate. The enzymatically hydrolyzed filtrate and the extraction filtrate are combined to obtain the combined filtrate. In the above technical solutions, vitamin C is a commonly used antioxidant. The purpose of adding vitamin C is to resist oxidation and prevent the active ingredients in the red algae, *Cyclocarya palustris*, from being oxidized. The purpose of cold plasma treatment is to destroy the lipid structure of cell membranes through active particles. The purpose of ultrasonic extraction is to avoid the destruction of heat-sensitive polysaccharides in the red algae, *Cyclocarya palustris*, by high temperatures.

[0012] S4. Fractional purification: After the combined filtrate is subjected to resin adsorption, impurities and target components are eluted, the eluent is collected, concentrated, and a concentrated solution is obtained. In the above technical solution, the purpose of concentration is to remove some organic solvent and reduce the amount of organic solvent, thereby facilitating the subsequent salting-out process.

[0013] S5. Directed precipitation of proteoglycans: The concentrate is subjected to salting out, alcohol precipitation, washing and drying to obtain purified proteoglycans; S6. Extraction of lipid-soluble active ingredients: The extraction residue is subjected to supercritical carbon dioxide extraction to separate the lipid-soluble active ingredients; S7. Compound formulation: The refined proteoglycan and fat-soluble active ingredients are mixed to obtain the extract of the red algae palm-leaf tree.

[0014] The extraction method of the extract of *Cymbidium goeringii* of the present invention first destroys the cell wall of *Cymbidium goeringii* through low-temperature enzymatic hydrolysis, and then obtains refined proteoglycans by ultrasonic-cold plasma combined extraction, purification and salting out; and then extracts the fat-soluble active ingredients in *Cymbidium goeringii* through supercritical carbon dioxide extraction, and then obtains the extract of *Cymbidium goeringii* through compound formulation, thereby significantly increasing the content of active ingredients in the extract of *Cymbidium goeringii* and achieving high extraction efficiency.

[0015] Preferably, in step S1, the small segment is 0.5-1 cm long, and the vacuum freeze-drying is carried out at -40°C and 0.01 MPa for 46-50 hours; the pulverization is ultra-fine pulverization, and the powder can pass through a 130-150 mesh sieve. Preferably, in step S2, the mass ratio of the powder to the buffer solution is 1:20, the pH of the buffer solution is 4.5 and it is pre-cooled at 2-6°C; the mass ratio of the powder to the complex enzyme is 1:0.05; the complex enzyme comprises 30% cellulase, 40% hemicellulase, and 30% pectinase by mass percentage; the low temperature is 23-27°C, and the enzymatic hydrolysis time is 1.3-1.7 hours; the low-frequency ultrasound has a frequency of 20kHz and a power of 150W; the filtration is performed using a 300-350 mesh filter membrane. Preferably, in step S3, the volume ratio of the enzymatically hydrolyzed filter residue or the obtained filter residue to the 30-40% ethanol-water mixed solvent is 1:18; the 30-40% ethanol-water mixed solvent contains vitamin C at a mass ratio of 0.1%; the cold plasma treatment is performed by using 80W helium plasma at a distance of 5cm from the liquid surface for 8-12 minutes; the ultrasonic extraction is performed at an ultrasonic frequency of 40kHz and a power of 250W, at 30-40℃ for 35-45 minutes.

[0016] Preferably, in step S4, the resin is an AB-8 macroporous resin column, and the liquid flow rate during resin adsorption is 1-3 BV / h; when eluting impurities, deionized water containing 5% ethanol is used, which is 5 times the volume of the macroporous resin column; when eluting the target component, a 55-65% ethanol aqueous solution is used; and the concentration is achieved by using a nanofiltration membrane with a molecular weight cutoff of 5000 Da to concentrate the eluent to 1 / 10 of its original volume. Preferably, in step S5, the protein in the concentrate is subjected to salting out, alcohol precipitation, washing, and drying. This involves adding ammonium sulfate to the concentrate until the saturation is 75-85%, letting it stand at 4°C for 10-14 hours, and then centrifuging at 8000 r / min for 13-17 minutes to obtain crude proteoglycan. Then, the crude proteoglycan is washed with 75% ethanol in a gradient 3-4 times and freeze-dried. Preferably, in step S6, the supercritical carbon dioxide extraction is performed at 30-35°C and 10-12 MPa, using 5% ethanol as an entrainer, and dynamically extracting for 80-100 minutes. Preferably, in step S7, the refined proteoglycan and the fat-soluble active ingredient are mixed at a mass ratio of 4:1.

[0017] Furthermore, the surfactant is one or more of sodium lauryl ether sulfate, sodium lauryl sulfate, cocamidopropyl betaine, sodium cocoyl glutamate, sodium lauroyl sarcosinate, fatty amide hydroxypropyl sulfobetaine, and sodium cocoyl aminopropionate.

[0018] Preferably, the surfactant is a compound of sodium lauryl ether sulfate, sodium lauryl sulfate, cocamidopropyl betaine, and sodium cocoaminopropionate, forming the basis of the cleaning and foaming system.

[0019] Furthermore, the moisturizer is one or more of glycerol, propylene glycol, sorbitol, 1,3-butanediol, 1,2-propanediol, and hydrolyzed keratin.

[0020] Preferably, the humectant is glycerol.

[0021] Furthermore, the hair conditioning agent is one or more of the following: hydroxypropyl guar hydroxypropyltrimethylammonium chloride, polyquaternium-11, polyquaternium-10, polyquaternium-7, PEG-7 glyceryl cocoate, myristamidopropyl PG-dimethylammonium chloride phosphate, and PPG-3 octyl ether.

[0022] Furthermore, the skin conditioning agent is one or more of betaine, sodium PCA, nicotinamide, and panthenol.

[0023] Furthermore, the preservative is one or more of phenoxyethanol, sodium benzoate, Kathon, benzyl alcohol, and ethylhexylglycerin; the pH adjuster is citric acid.

[0024] On the other hand, the present invention also provides a method for preparing the above-mentioned shampoo and hair care composition, comprising the following steps: a. Add the surfactant to deionized water at 85℃-90℃, stir evenly, and then cool to 80℃~85℃ and keep warm to obtain material 1; b. Add hair conditioning agent to material 1, homogenize and keep warm. After homogenization until there are no particles, cool down to 40℃~45℃ to obtain material 2. c. Add the extracts of red algae palmatum, patchouli leaf extract, skin conditioning agent, moisturizer, fragrance agent, and preservative to material 2 and stir well. Add a pH adjuster to adjust the pH value to 5.5-6.0 to obtain the shampoo and hair care composition.

[0025] By controlling the pH value of the shampoo and conditioner composition of the present invention between 5.5 and 6.0, which conforms to the slightly acidic environment of the scalp, it helps maintain the stability of the scalp barrier, avoids damage to the surface sebum film, and reduces product irritation. The preparation method of the shampoo and conditioner composition of the present invention is simple and suitable for industrial production.

[0026] The present invention has the following beneficial effects: 1. The shampoo and conditioner composition containing extract of *Pterocarya stenoptera* of the present invention, through the rational construction of the shampoo and conditioner composition system architecture, uses extract of *Pterocarya stenoptera* as the core active ingredient, and combines it with patchouli leaf extract to effectively improve the oil control and dandruff removal effect of the shampoo and conditioner composition. The resulting shampoo and conditioner composition has a moderate scalp cleansing and conditioning effect, is safe and gentle, and has a good foaming feel. After use, the combability of dry and wet hair is significantly improved, which can delicately nourish hair strands, smooth damaged hair cuticles, restore the hair's watery texture, and improve frizz and tangles.

[0027] 2. The extraction method of the extract of *Cymbidium goeringii* of the present invention first destroys the cell wall of *Cymbidium goeringii* by low-temperature enzymatic hydrolysis, and then obtains refined proteoglycans by ultrasonic-cold plasma combined extraction, purification and salting out; and then extracts the fat-soluble active ingredients in *Cymbidium goeringii* by supercritical carbon dioxide extraction, and then obtains the extract of *Cymbidium goeringii* by compounding, thereby significantly increasing the content of active ingredients in the extract of *Cymbidium goeringii* and achieving high extraction efficiency.

[0028] 3. The preparation method of the shampoo and hair care composition of the present invention is simple and suitable for industrial production. Detailed Implementation

[0029] To make the technical problems, solutions, and advantages of this invention clearer, a detailed description will be provided below with reference to specific examples. However, the scope of protection of this invention is not limited to the following specific embodiments. The described embodiments are merely some, not all, of the embodiments of this invention, and are not intended to limit the invention. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0030] Unless otherwise defined, all technical terms used herein have the same meaning as commonly understood by those skilled in the art. The technical terms used herein are for the purpose of describing particular embodiments only and are not intended to limit the scope of the invention.

[0031] Unless otherwise specified, all raw materials, reagents, instruments and equipment used in this invention can be purchased from the market or prepared by existing methods.

[0032] Examples 1 to 4 of the present invention provide a shampoo and hair care composition, and Comparative Examples 1 to 3 provide a shampoo composition. The dosage formulas of the raw materials used in their preparation are shown in Table 1 below; the units are by mass percentage.

[0033] Table 1. Raw material formulations for Examples 1-4 and Comparative Examples 1-3

[0034] The extraction method for the extract of the red algae *Cinnamomum camphora* used in the above embodiments and comparative examples includes the following steps: S1. Raw material pretreatment: Take the whole fresh red algae palm leaf tree, wash it, cut it into small sections of 0.5-1cm in length, and freeze-dry it under vacuum at -40℃ and 0.01MPa for 48 hours, and then pulverize it into powder that can pass through a 150-mesh sieve. S2. Low-temperature enzymatic hydrolysis: The powder is transferred into a low-temperature reaction vessel, protected by an inert gas atmosphere. A citrate-sodium citrate buffer solution is added, followed by the addition of a complex enzyme. Enzymatic hydrolysis is then performed at low temperature with the assistance of low-frequency ultrasound. The resulting hydrolysate is filtered to obtain a residue and a filtrate. The mass ratio of the powder to the buffer solution is 1:20, the pH of the buffer solution is 4.5, and it is pre-cooled to 4°C. The mass ratio of the powder to the complex enzyme is 1:0.05. The complex enzyme comprises 30% cellulase, 40% hemicellulase, and 30% pectinase by mass percentage. The low-temperature setting is 25°C, and the hydrolysis time is 1.5 hours. The low-frequency ultrasound frequency is 20kHz, and the power is 150W. Filtration is performed using a 300-mesh filter membrane. S3. Combined Ultrasonic-Cold Plasma Extraction: The enzymatically hydrolyzed filter residue is added to a 30% ethanol-water mixed solvent containing vitamin C and subjected to cold plasma treatment. Ultrasonic extraction is then initiated, followed by filtration. The obtained filter residue is added to a 30-40% ethanol-water mixed solvent containing vitamin C, and the ultrasonic extraction and filtration are repeated twice to obtain the extracted filter residue and filtrate. The multiple filtrates are combined to obtain the extracted filtrate, and the enzymatically hydrolyzed filtrate and the extracted filtrate are combined to obtain the combined filtrate. The volume ratio of the enzymatically hydrolyzed filter residue or the obtained filter residue to the 30% ethanol-water mixed solvent is 1:18. The 30% ethanol-water mixed solvent contains 0.1% vitamin C by mass. The cold plasma treatment uses 80W helium plasma at a distance of 5cm from the liquid surface for 10 minutes. The ultrasonic extraction is performed at a frequency of 40kHz and a power of 250W at 35℃ for 40 minutes. S4. Fractional Purification: The combined filtrate is subjected to resin adsorption, elution of impurities and target components, and the eluent is collected and concentrated to obtain a concentrate. The resin is an AB-8 macroporous resin column, and the liquid flow rate during resin adsorption is 2 BV / h. The impurities are eluted using 5 times the volume of deionized water containing 5% ethanol, and the target components are eluted using a 60% ethanol aqueous solution. The concentration is achieved by using a nanofiltration membrane with a molecular weight cutoff of 5000 Da to concentrate the eluent to 1 / 10 of its original volume. S5. Directed precipitation of proteoglycans: The concentrate was subjected to salting out, alcohol precipitation, washing, and drying to obtain purified proteoglycans; wherein, the salting out, alcohol precipitation, washing, and drying of the concentrate was performed by adding ammonium sulfate to the concentrate to 80% saturation, letting it stand at 4°C for 12 hours, and then centrifuging at 8000 r / min for 15 minutes to obtain crude proteoglycans; then, the crude proteoglycans were washed three times with 75% ethanol in a gradient and then freeze-dried; S6. Extraction of lipid-soluble active ingredients: The extraction residue is subjected to supercritical carbon dioxide extraction to separate the lipid-soluble active ingredients; wherein, the supercritical carbon dioxide extraction is performed at 35℃ and 12MPa, with 5% ethanol as an entrainer, for 90 minutes of dynamic extraction. S7. Compound formulation: The refined proteoglycan and the fat-soluble active ingredients are mixed at a mass ratio of 4:1 to obtain the extract of the red algae palm-leaf tree.

[0035] The preparation method of the shampoo and conditioner composition of Example 1 above includes the following steps: a. Add surfactants (sodium lauryl ether sulfate, sodium lauryl sulfate, cocamidopropyl betaine, sodium cocoaminopropionate) to deionized water at 90°C, stir evenly, and then cool to 85°C and keep warm to obtain material 1. b. Add hair conditioning agent (polyquaternium-10, PPG-3 octyl ether) to material 1, homogenize and keep warm. After homogenization until there are no particles, cool down to 45°C to obtain material 2. c. Add the extracts of red algae palmatum, patchouli leaf extract, skin conditioning agent (sodium PCA), moisturizer (glycerol), fragrance agent, and preservative (phenoxyethanol, sodium benzoate) to material 2 and stir well. Then add a pH adjuster (citric acid) to adjust the pH value to 5.5 to obtain the shampoo and hair care composition.

[0036] The methods for preparing shampoo and hair care compositions in Examples 2-4 and Comparative Examples 1-3 can be obtained in the same way. If there are components that are not added or replaced, they can be deleted or replaced in the corresponding preparation steps.

[0037] The efficacy of the products prepared in Examples 1-4 and Comparative Examples 1-3 was evaluated by instrument efficacy testing and human sensory evaluation. The foam height, foam half-life and chicken embryo irritation of the samples were also tested. The test methods are as follows, and the test results are shown in Table 2.

[0038] (1) Scalp oil improvement rate, dandruff removal effect, and soothing feedback score test: Seventy volunteers with symptoms of itchy scalp and dandruff were randomly selected and randomly divided into seven groups of 10 people each. The first group used the shampoo and conditioner composition of Example 1, the second group used the shampoo and conditioner composition of Example 2, and so on. They shampooed their hair once a day for seven consecutive days. The scalp oil content before sample application and 3 hours after the last use of the shampoo and hair care composition were measured, and the scalp oil improvement rate was calculated according to the change rate calculation formula. The scalp oil content test method is a well-known test method in the art and will not be described in detail here. The ASFS (adherent scalp flaking score) values ​​of the volunteers were recorded before and after one week of continuous use of the shampoo and hair care composition. The dandruff improvement rate was calculated according to the rate of change formula. The specific testing method was as follows: The subjects did not wash their hair the day before the test. Before the test, the subjects sat quietly for at least 10 minutes in a laboratory at 20°C~25°C and 40%~60% relative humidity, without drinking water or beverages, with their hair loose and in the test position, remaining relaxed. Subjects were screened by observing the test sites, selecting those with a dandruff score of 3 or higher. Four areas with severe dandruff were selected from the subjects' assessment areas, and these four areas were assessed at each time point. Sample application (application site, amount, and method): After screening, the sample was dried in the laboratory, and photos were taken immediately for follow-up. The subjects then used the sample at home for one week (a total of 3 times, including one time in the laboratory). Subjects were required to apply an appropriate amount of sample evenly to wet hair according to the product usage instructions, gently lather, massage thoroughly, and then rinse with water. During the trial, participants followed the instructions and were revisited one week after using the sample. They did not wash their hair the day before the test to assess their dandruff condition and take photos of the dandruff at the test points using an instrument.

[0039] The soothing feedback score is the human sensory evaluation of the above subjects, with a total score of 100 points. The higher the score, the better the effect.

[0040] (2) Test of the change rate of combing force of dry and wet hair: The combing force of wet hair and the combing force of dry hair were measured using healthy human hair strands. The specific test method is a well-known test method in the field and will not be described in detail here. After the test, the change rate of combing force before and after using the sample was calculated according to the change rate calculation formula. (3) Foam height and foam half-life test: According to the determination method of GB / T 13173-2008, take 2.5g of each of the shampoo and conditioning compositions prepared in the examples and comparative examples, dissolve them in 150mg / kg hard water, transfer them to 1000mL volumetric flasks, dilute to the mark, shake well, and then place the solution in a constant temperature water bath at (40±0.5)℃ for aging. The total time from the start of adding water to dissolve the sample is 30min. Then, use a Roche foam meter to measure the foam height and record the foam half-life. (4) Chicken embryo irritation test: The shampoo and conditioner compositions obtained in the examples and comparative examples were subjected to a chicken embryo irritation test. This method is a recognized test method in the art, and the specific steps are not described here. In this test, 0 points indicates no irritation, 5 points indicates severe capillary bleeding, and the higher the score, the stronger the irritation.

[0041] Table 2 Performance test results of Examples 1-4 and Comparative Examples 1-3

[0042] According to the test results in Table 2, compared with Comparative Examples 1-3, after using the shampoo and hair care compositions of Examples 1-4 of the present invention for a week, the scalp oil content was significantly reduced and the dandruff problem was significantly improved within 3 hours, indicating that the shampoo and hair care compositions of Examples 1-4 exhibited clear oil control and dandruff removal effects.

[0043] By comparing Example 2 with Comparative Examples 1 and 2, it can be seen that the combination of the extract of the red algae palmatum and the extract of patchouli leaves in the shampoo and hair care composition exhibits a synergistic effect. It can not only enhance the oil control, dandruff removal and soothing effects of the product, but also improve the foam texture during shampooing, enhance foam stability, and significantly improve the combability of hair in both dry and wet states, making the hair smoother.

[0044] A comparison of Examples 1-4 shows that the effect is better when the amount of the extract of the red algae palm leaf tree is in the range of 4%-6%. Further increasing the amount of the extract has a gradual effect on the improvement of oil control and other properties of the shampoo and hair care composition.

[0045] By comparing Examples 1-4 with Comparative Examples 1-3, it can be seen that the shampoo and hair care composition of the present invention has low irritation and high mildness and safety, making it more suitable for use on sensitive scalps.

[0046] This invention, through the rational construction of a shampoo and conditioner composition system, uses the extract of the red algae palmatum as the main active ingredient, combined with patchouli leaf extract. When these two are combined, the oil-controlling and dandruff-removing effects of the shampoo and conditioner composition can be effectively improved. The resulting shampoo and conditioner composition has a moderate scalp cleansing and conditioning effect, is safe and gentle, and has a good foaming feel. After use, the combability of dry and wet hair is significantly improved, which can delicately nourish hair strands, smooth damaged hair cuticles, restore the hair's moisture and texture, and improve frizz and tangles.

[0047] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details of the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0048] Furthermore, it should be noted that the various specific technical features described in the above embodiments can be combined in any suitable manner without contradiction. To avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

[0049] Furthermore, various different embodiments of the present invention can be combined in any way, as long as they do not violate the spirit of the present invention, they should also be regarded as the content disclosed by the present invention.

Claims

1. A shampoo and conditioner composition containing extract of the red algae *Phyllostachys edulis*, characterized in that, The preparation materials include the following raw materials by weight percentage: 0.1%–10% of *Rhododendron palmatum* extract; 0.1%–0.5% of *Pogostemon cablin* leaf extract; 14%–25% of surfactant; 0.3%–5% of moisturizer; 0.1%–1% of hair conditioner; 0.5%–1% of skin conditioner; 0.5%–1% of preservative; 0.5%–1% of fragrance agent; 0.01%–0.2% of pH adjuster; and the balance is made up to 100% with deionized water.

2. The shampoo and conditioner composition according to claim 1, characterized in that, The extraction method for the extract of the red algae *Callicarpa arborescens* includes the following steps: S1. Raw material pretreatment: Take the whole fresh red algae palm leaf tree, wash it, cut it into small sections, freeze dry it under vacuum, and then grind it into powder; S2. Low-temperature enzymatic hydrolysis: The powder is transferred into a low-temperature reaction vessel, protected by an inert gas, and a citrate-sodium citrate buffer solution is added. Then, a compound enzyme is added, and enzymatic hydrolysis is carried out at low temperature with the assistance of low-frequency ultrasound. The obtained enzymatic hydrolysate is filtered to obtain the enzymatic hydrolysis residue and the enzymatic hydrolysis filtrate. S3. Combined ultrasonic-cold plasma extraction: The enzymatically hydrolyzed filter residue is added to a 30-40% ethanol-water mixed solvent containing vitamin C and subjected to cold plasma treatment. Then, ultrasonic extraction is started, followed by filtration. The obtained filter residue is added to a 30-40% ethanol-water mixed solvent containing vitamin C, and ultrasonic extraction and filtration are repeated 2-3 times to obtain the extraction filter residue and filtrate. The multiple filtrates are combined to obtain the extraction filtrate. The enzymatically hydrolyzed filtrate and the extraction filtrate are combined to obtain the combined filtrate. S4. Fractional purification: After the combined filtrate is subjected to resin adsorption, impurities and target components are eluted, the eluent is collected, concentrated, and a concentrated solution is obtained. S5. Directed precipitation of proteoglycans: The concentrate is subjected to salting out, alcohol precipitation, washing and drying to obtain purified proteoglycans; S6. Extraction of lipid-soluble active ingredients: The extraction residue is subjected to supercritical carbon dioxide extraction to separate the lipid-soluble active ingredients; S7. Compound formulation: The refined proteoglycan and fat-soluble active ingredients are mixed to obtain the extract of the red algae palm-leaf tree.

3. The shampoo and conditioner composition according to claim 2, characterized in that, In step S1, the small segment is 0.5-1 cm in length, and it is freeze-dried at -40°C. Freeze-dry at 0.01 MPa for 46-50 hours; the pulverization is ultrafine pulverization, and the powder can pass through a 130-150 mesh sieve; In step S2, the mass ratio of the powder to the buffer solution is 1:20, the pH of the buffer solution is 4.5 and it is pre-cooled at 2-6℃; the mass ratio of the powder to the complex enzyme is 1:0.05; the complex enzyme comprises 30% cellulase, 40% hemicellulase, and 30% pectinase by mass percentage; the low temperature is 23-27℃, and the enzymatic hydrolysis time is 1.3-1.7 hours; the low-frequency ultrasound has a frequency of 20kHz and a power of 150W; the filtration is performed using a 300-350 mesh filter membrane. In step S3, the volume ratio of the enzymatically hydrolyzed filter residue or the obtained filter residue to the 30-40% ethanol-water mixed solvent is 1:18; the 30-40% ethanol-water mixed solvent contains vitamin C at a mass ratio of 0.1%; the cold plasma treatment is performed by using 80W helium plasma at a distance of 5cm from the liquid surface for 8-12 minutes; the ultrasonic extraction is performed at an ultrasonic frequency of 40kHz and a power of 250W, at 30-40℃ for 35-45 minutes.

4. The shampoo and conditioner composition according to claim 2, characterized in that, In step S4, the resin is an AB-8 macroporous resin column, and the liquid flow rate during resin adsorption is 1-3 BV / h; when eluting impurities, deionized water containing 5% ethanol is used, which is 5 times the volume of the macroporous resin column; when eluting the target component, 55-65% ethanol aqueous solution is used; and the concentration is achieved by using a nanofiltration membrane with a molecular weight cutoff of 5000 Da to concentrate the eluent to 1 / 10 of its original volume. In step S5, the concentrate is subjected to salting out, alcohol precipitation, washing, and drying. This involves adding ammonium sulfate to the concentrate until the saturation reaches 75-85%, allowing it to stand at 4°C for 10-14 hours, and then centrifuging at 8000 r / min for 13-17 minutes to obtain crude proteoglycans. The crude proteoglycans are then washed with 75% ethanol in a gradient 3-4 times and freeze-dried. In step S6, the supercritical carbon dioxide extraction is performed at 30-35℃ and 10-12MPa, using 5% ethanol as an entrainer, and dynamically extracting for 80-100 minutes. In step S7, the refined proteoglycan and the fat-soluble active ingredient are mixed at a mass ratio of 4:

1.

5. The shampoo and conditioner composition according to claim 1, characterized in that, The surfactant is one or more of sodium lauryl ether sulfate, sodium lauryl sulfate, cocamidopropyl betaine, sodium cocoyl glutamate, sodium lauroyl sarcosinate, fatty amide hydroxypropyl sulfobetaine, and sodium cocoyl aminopropionate.

6. The shampoo and conditioner composition according to claim 1, characterized in that, The moisturizer is one or more of glycerol, propylene glycol, sorbitol, 1,3-butanediol, 1,2-propanediol, and hydrolyzed keratin.

7. The shampoo and conditioner composition according to claim 1, characterized in that, The hair conditioning agent is one or more of the following: hydroxypropyl guar hydroxypropyl trimethylammonium chloride, polyquaternium-11, polyquaternium-10, polyquaternium-7, PEG-7 glyceryl cocoate, myristamidopropyl PG-dimethylammonium chloride phosphate, and PPG-3 octyl ether.

8. The shampoo and conditioner composition according to claim 1, characterized in that, The skin conditioning agent is one or more of betaine, sodium PCA, nicotinamide, and panthenol.

9. The shampoo and conditioner composition according to claim 1, characterized in that, The preservative is one or more of phenoxyethanol, sodium benzoate, Kathon, benzyl alcohol, and ethylhexylglycerin; the pH adjuster is citric acid.

10. A method for preparing a shampoo and conditioner composition according to any one of claims 1-9, comprising the following steps: a. Add the surfactant to deionized water at 85℃-90℃, stir evenly, and then cool to 80℃~85℃ and keep warm to obtain material 1; b. Add hair conditioning agent to material 1, homogenize and keep warm. After homogenization until there are no particles, cool down to 40℃~45℃ to obtain material 2. c. Add the extracts of red algae palmatum, patchouli leaf extract, skin conditioning agent, moisturizer, fragrance agent, and preservative to material 2 and stir well. Add a pH adjuster to adjust the pH value to 5.5-6.0 to obtain the shampoo and hair care composition.

Images